Towards the Laboratory Maintenance of <i<Haemagogus janthinomys</i< (Dyar, 1921), the Major Neotropical Vector of Sylvatic Yellow Fever

<i<Haemagogus (Haemagogus) janthinomys</i< (Dyar, 1921), the major neotropical vector of sylvatic yellow fever virus, is notoriously difficult to maintain in captivity. It has never been reared beyond an F<sub<1</sub< generation, and almost no experimental transmission studie...
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Autor*in:	Adam Hendy [verfasserIn] Nelson Ferreira Fé [verfasserIn] Danielle Valério [verfasserIn] Eduardo Hernandez-Acosta [verfasserIn] Bárbara A. Chaves [verfasserIn] Luís Felipe Alho da Silva [verfasserIn] Rosa Amélia Gonçalves Santana [verfasserIn] Andréia da Costa Paz [verfasserIn] Matheus Mickael Mota Soares [verfasserIn] Flamarion Prado Assunção [verfasserIn] José Tenaçol Andes [verfasserIn] Chiara Andolina [verfasserIn] Vera Margarete Scarpassa [verfasserIn] Marcus Vinícius Guimarães de Lacerda [verfasserIn] Kathryn A. Hanley [verfasserIn] Nikos Vasilakis [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	sylvatic arbovirus yellow fever Flaviviridae

Übergeordnetes Werk:	In: Viruses - MDPI AG, 2009, 15(2022), 1, p 45
Übergeordnetes Werk:	volume:15 ; year:2022 ; number:1, p 45

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/v15010045

Katalog-ID:	DOAJ081686528

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allfields	10.3390/v15010045 doi (DE-627)DOAJ081686528 (DE-599)DOAJ1c0040413dd74fba814d8cc111a7742b DE-627 ger DE-627 rakwb eng QR1-502 Adam Hendy verfasserin aut Towards the Laboratory Maintenance of <i<Haemagogus janthinomys</i< (Dyar, 1921), the Major Neotropical Vector of Sylvatic Yellow Fever 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Haemagogus (Haemagogus) janthinomys</i< (Dyar, 1921), the major neotropical vector of sylvatic yellow fever virus, is notoriously difficult to maintain in captivity. It has never been reared beyond an F<sub<1</sub< generation, and almost no experimental transmission studies have been performed with this species since the 1940s. Herein we describe installment hatching, artificial blood feeding, and forced-mating techniques that enabled us to produce small numbers of F<sub<3</sub< generation <i<Hg. janthinomys</i< eggs for the first time. A total of 62.8% (1562/2486) F<sub<1</sub< generation eggs hatched during ≤10 four-day cycles of immersion in a bamboo leaf infusion followed by partial drying. Hatching decreased to 20.1% (190/944) in the F<sub<2</sub< generation for eggs laid by mosquitoes copulated by forced mating. More than 85% (79/92) female F<sub<2</sub< mosquitoes fed on an artificial blood feeding system. While we were unable to maintain a laboratory colony of <i<Hg. janthinomys</i< past the F<sub<3</sub< generation, our methods provide a foundation for experimental transmission studies with this species in a laboratory setting, a critical capacity in a region with hyper-endemic transmission of dengue, Zika, and chikungunya viruses, all posing a risk of spillback into a sylvatic cycle. <i<Haemagogus janthinomys</i< sylvatic arbovirus yellow fever Flaviviridae <i<Flavivirus</i< Microbiology Nelson Ferreira Fé verfasserin aut Danielle Valério verfasserin aut Eduardo Hernandez-Acosta verfasserin aut Bárbara A. Chaves verfasserin aut Luís Felipe Alho da Silva verfasserin aut Rosa Amélia Gonçalves Santana verfasserin aut Andréia da Costa Paz verfasserin aut Matheus Mickael Mota Soares verfasserin aut Flamarion Prado Assunção verfasserin aut José Tenaçol Andes verfasserin aut Chiara Andolina verfasserin aut Vera Margarete Scarpassa verfasserin aut Marcus Vinícius Guimarães de Lacerda verfasserin aut Kathryn A. Hanley verfasserin aut Nikos Vasilakis verfasserin aut In Viruses MDPI AG, 2009 15(2022), 1, p 45 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:15 year:2022 number:1, p 45 https://doi.org/10.3390/v15010045 kostenfrei https://doaj.org/article/1c0040413dd74fba814d8cc111a7742b kostenfrei https://www.mdpi.com/1999-4915/15/1/45 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 1, p 45
spelling	10.3390/v15010045 doi (DE-627)DOAJ081686528 (DE-599)DOAJ1c0040413dd74fba814d8cc111a7742b DE-627 ger DE-627 rakwb eng QR1-502 Adam Hendy verfasserin aut Towards the Laboratory Maintenance of <i<Haemagogus janthinomys</i< (Dyar, 1921), the Major Neotropical Vector of Sylvatic Yellow Fever 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Haemagogus (Haemagogus) janthinomys</i< (Dyar, 1921), the major neotropical vector of sylvatic yellow fever virus, is notoriously difficult to maintain in captivity. It has never been reared beyond an F<sub<1</sub< generation, and almost no experimental transmission studies have been performed with this species since the 1940s. Herein we describe installment hatching, artificial blood feeding, and forced-mating techniques that enabled us to produce small numbers of F<sub<3</sub< generation <i<Hg. janthinomys</i< eggs for the first time. A total of 62.8% (1562/2486) F<sub<1</sub< generation eggs hatched during ≤10 four-day cycles of immersion in a bamboo leaf infusion followed by partial drying. Hatching decreased to 20.1% (190/944) in the F<sub<2</sub< generation for eggs laid by mosquitoes copulated by forced mating. More than 85% (79/92) female F<sub<2</sub< mosquitoes fed on an artificial blood feeding system. While we were unable to maintain a laboratory colony of <i<Hg. janthinomys</i< past the F<sub<3</sub< generation, our methods provide a foundation for experimental transmission studies with this species in a laboratory setting, a critical capacity in a region with hyper-endemic transmission of dengue, Zika, and chikungunya viruses, all posing a risk of spillback into a sylvatic cycle. <i<Haemagogus janthinomys</i< sylvatic arbovirus yellow fever Flaviviridae <i<Flavivirus</i< Microbiology Nelson Ferreira Fé verfasserin aut Danielle Valério verfasserin aut Eduardo Hernandez-Acosta verfasserin aut Bárbara A. Chaves verfasserin aut Luís Felipe Alho da Silva verfasserin aut Rosa Amélia Gonçalves Santana verfasserin aut Andréia da Costa Paz verfasserin aut Matheus Mickael Mota Soares verfasserin aut Flamarion Prado Assunção verfasserin aut José Tenaçol Andes verfasserin aut Chiara Andolina verfasserin aut Vera Margarete Scarpassa verfasserin aut Marcus Vinícius Guimarães de Lacerda verfasserin aut Kathryn A. Hanley verfasserin aut Nikos Vasilakis verfasserin aut In Viruses MDPI AG, 2009 15(2022), 1, p 45 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:15 year:2022 number:1, p 45 https://doi.org/10.3390/v15010045 kostenfrei https://doaj.org/article/1c0040413dd74fba814d8cc111a7742b kostenfrei https://www.mdpi.com/1999-4915/15/1/45 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 1, p 45
allfields_unstemmed	10.3390/v15010045 doi (DE-627)DOAJ081686528 (DE-599)DOAJ1c0040413dd74fba814d8cc111a7742b DE-627 ger DE-627 rakwb eng QR1-502 Adam Hendy verfasserin aut Towards the Laboratory Maintenance of <i<Haemagogus janthinomys</i< (Dyar, 1921), the Major Neotropical Vector of Sylvatic Yellow Fever 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Haemagogus (Haemagogus) janthinomys</i< (Dyar, 1921), the major neotropical vector of sylvatic yellow fever virus, is notoriously difficult to maintain in captivity. It has never been reared beyond an F<sub<1</sub< generation, and almost no experimental transmission studies have been performed with this species since the 1940s. Herein we describe installment hatching, artificial blood feeding, and forced-mating techniques that enabled us to produce small numbers of F<sub<3</sub< generation <i<Hg. janthinomys</i< eggs for the first time. A total of 62.8% (1562/2486) F<sub<1</sub< generation eggs hatched during ≤10 four-day cycles of immersion in a bamboo leaf infusion followed by partial drying. Hatching decreased to 20.1% (190/944) in the F<sub<2</sub< generation for eggs laid by mosquitoes copulated by forced mating. More than 85% (79/92) female F<sub<2</sub< mosquitoes fed on an artificial blood feeding system. While we were unable to maintain a laboratory colony of <i<Hg. janthinomys</i< past the F<sub<3</sub< generation, our methods provide a foundation for experimental transmission studies with this species in a laboratory setting, a critical capacity in a region with hyper-endemic transmission of dengue, Zika, and chikungunya viruses, all posing a risk of spillback into a sylvatic cycle. <i<Haemagogus janthinomys</i< sylvatic arbovirus yellow fever Flaviviridae <i<Flavivirus</i< Microbiology Nelson Ferreira Fé verfasserin aut Danielle Valério verfasserin aut Eduardo Hernandez-Acosta verfasserin aut Bárbara A. Chaves verfasserin aut Luís Felipe Alho da Silva verfasserin aut Rosa Amélia Gonçalves Santana verfasserin aut Andréia da Costa Paz verfasserin aut Matheus Mickael Mota Soares verfasserin aut Flamarion Prado Assunção verfasserin aut José Tenaçol Andes verfasserin aut Chiara Andolina verfasserin aut Vera Margarete Scarpassa verfasserin aut Marcus Vinícius Guimarães de Lacerda verfasserin aut Kathryn A. Hanley verfasserin aut Nikos Vasilakis verfasserin aut In Viruses MDPI AG, 2009 15(2022), 1, p 45 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:15 year:2022 number:1, p 45 https://doi.org/10.3390/v15010045 kostenfrei https://doaj.org/article/1c0040413dd74fba814d8cc111a7742b kostenfrei https://www.mdpi.com/1999-4915/15/1/45 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 1, p 45
allfieldsGer	10.3390/v15010045 doi (DE-627)DOAJ081686528 (DE-599)DOAJ1c0040413dd74fba814d8cc111a7742b DE-627 ger DE-627 rakwb eng QR1-502 Adam Hendy verfasserin aut Towards the Laboratory Maintenance of <i<Haemagogus janthinomys</i< (Dyar, 1921), the Major Neotropical Vector of Sylvatic Yellow Fever 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Haemagogus (Haemagogus) janthinomys</i< (Dyar, 1921), the major neotropical vector of sylvatic yellow fever virus, is notoriously difficult to maintain in captivity. It has never been reared beyond an F<sub<1</sub< generation, and almost no experimental transmission studies have been performed with this species since the 1940s. Herein we describe installment hatching, artificial blood feeding, and forced-mating techniques that enabled us to produce small numbers of F<sub<3</sub< generation <i<Hg. janthinomys</i< eggs for the first time. A total of 62.8% (1562/2486) F<sub<1</sub< generation eggs hatched during ≤10 four-day cycles of immersion in a bamboo leaf infusion followed by partial drying. Hatching decreased to 20.1% (190/944) in the F<sub<2</sub< generation for eggs laid by mosquitoes copulated by forced mating. More than 85% (79/92) female F<sub<2</sub< mosquitoes fed on an artificial blood feeding system. While we were unable to maintain a laboratory colony of <i<Hg. janthinomys</i< past the F<sub<3</sub< generation, our methods provide a foundation for experimental transmission studies with this species in a laboratory setting, a critical capacity in a region with hyper-endemic transmission of dengue, Zika, and chikungunya viruses, all posing a risk of spillback into a sylvatic cycle. <i<Haemagogus janthinomys</i< sylvatic arbovirus yellow fever Flaviviridae <i<Flavivirus</i< Microbiology Nelson Ferreira Fé verfasserin aut Danielle Valério verfasserin aut Eduardo Hernandez-Acosta verfasserin aut Bárbara A. Chaves verfasserin aut Luís Felipe Alho da Silva verfasserin aut Rosa Amélia Gonçalves Santana verfasserin aut Andréia da Costa Paz verfasserin aut Matheus Mickael Mota Soares verfasserin aut Flamarion Prado Assunção verfasserin aut José Tenaçol Andes verfasserin aut Chiara Andolina verfasserin aut Vera Margarete Scarpassa verfasserin aut Marcus Vinícius Guimarães de Lacerda verfasserin aut Kathryn A. Hanley verfasserin aut Nikos Vasilakis verfasserin aut In Viruses MDPI AG, 2009 15(2022), 1, p 45 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:15 year:2022 number:1, p 45 https://doi.org/10.3390/v15010045 kostenfrei https://doaj.org/article/1c0040413dd74fba814d8cc111a7742b kostenfrei https://www.mdpi.com/1999-4915/15/1/45 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 1, p 45
allfieldsSound	10.3390/v15010045 doi (DE-627)DOAJ081686528 (DE-599)DOAJ1c0040413dd74fba814d8cc111a7742b DE-627 ger DE-627 rakwb eng QR1-502 Adam Hendy verfasserin aut Towards the Laboratory Maintenance of <i<Haemagogus janthinomys</i< (Dyar, 1921), the Major Neotropical Vector of Sylvatic Yellow Fever 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Haemagogus (Haemagogus) janthinomys</i< (Dyar, 1921), the major neotropical vector of sylvatic yellow fever virus, is notoriously difficult to maintain in captivity. It has never been reared beyond an F<sub<1</sub< generation, and almost no experimental transmission studies have been performed with this species since the 1940s. Herein we describe installment hatching, artificial blood feeding, and forced-mating techniques that enabled us to produce small numbers of F<sub<3</sub< generation <i<Hg. janthinomys</i< eggs for the first time. A total of 62.8% (1562/2486) F<sub<1</sub< generation eggs hatched during ≤10 four-day cycles of immersion in a bamboo leaf infusion followed by partial drying. Hatching decreased to 20.1% (190/944) in the F<sub<2</sub< generation for eggs laid by mosquitoes copulated by forced mating. More than 85% (79/92) female F<sub<2</sub< mosquitoes fed on an artificial blood feeding system. While we were unable to maintain a laboratory colony of <i<Hg. janthinomys</i< past the F<sub<3</sub< generation, our methods provide a foundation for experimental transmission studies with this species in a laboratory setting, a critical capacity in a region with hyper-endemic transmission of dengue, Zika, and chikungunya viruses, all posing a risk of spillback into a sylvatic cycle. <i<Haemagogus janthinomys</i< sylvatic arbovirus yellow fever Flaviviridae <i<Flavivirus</i< Microbiology Nelson Ferreira Fé verfasserin aut Danielle Valério verfasserin aut Eduardo Hernandez-Acosta verfasserin aut Bárbara A. Chaves verfasserin aut Luís Felipe Alho da Silva verfasserin aut Rosa Amélia Gonçalves Santana verfasserin aut Andréia da Costa Paz verfasserin aut Matheus Mickael Mota Soares verfasserin aut Flamarion Prado Assunção verfasserin aut José Tenaçol Andes verfasserin aut Chiara Andolina verfasserin aut Vera Margarete Scarpassa verfasserin aut Marcus Vinícius Guimarães de Lacerda verfasserin aut Kathryn A. Hanley verfasserin aut Nikos Vasilakis verfasserin aut In Viruses MDPI AG, 2009 15(2022), 1, p 45 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:15 year:2022 number:1, p 45 https://doi.org/10.3390/v15010045 kostenfrei https://doaj.org/article/1c0040413dd74fba814d8cc111a7742b kostenfrei https://www.mdpi.com/1999-4915/15/1/45 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 1, p 45
language	English
source	In Viruses 15(2022), 1, p 45 volume:15 year:2022 number:1, p 45
sourceStr	In Viruses 15(2022), 1, p 45 volume:15 year:2022 number:1, p 45
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<Haemagogus janthinomys</i< sylvatic arbovirus yellow fever Flaviviridae <i<Flavivirus</i< Microbiology
isfreeaccess_bool	true
container_title	Viruses
authorswithroles_txt_mv	Adam Hendy @@aut@@ Nelson Ferreira Fé @@aut@@ Danielle Valério @@aut@@ Eduardo Hernandez-Acosta @@aut@@ Bárbara A. Chaves @@aut@@ Luís Felipe Alho da Silva @@aut@@ Rosa Amélia Gonçalves Santana @@aut@@ Andréia da Costa Paz @@aut@@ Matheus Mickael Mota Soares @@aut@@ Flamarion Prado Assunção @@aut@@ José Tenaçol Andes @@aut@@ Chiara Andolina @@aut@@ Vera Margarete Scarpassa @@aut@@ Marcus Vinícius Guimarães de Lacerda @@aut@@ Kathryn A. Hanley @@aut@@ Nikos Vasilakis @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	609775871
id	DOAJ081686528
language_de	englisch
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callnumber-first	Q - Science
author	Adam Hendy
spellingShingle	Adam Hendy misc QR1-502 misc <i<Haemagogus janthinomys</i< misc sylvatic misc arbovirus misc yellow fever misc Flaviviridae misc <i<Flavivirus</i< misc Microbiology Towards the Laboratory Maintenance of <i<Haemagogus janthinomys</i< (Dyar, 1921), the Major Neotropical Vector of Sylvatic Yellow Fever
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topic_title	QR1-502 Towards the Laboratory Maintenance of <i<Haemagogus janthinomys</i< (Dyar, 1921), the Major Neotropical Vector of Sylvatic Yellow Fever <i<Haemagogus janthinomys</i< sylvatic arbovirus yellow fever Flaviviridae <i<Flavivirus</i<
topic	misc QR1-502 misc <i<Haemagogus janthinomys</i< misc sylvatic misc arbovirus misc yellow fever misc Flaviviridae misc <i<Flavivirus</i< misc Microbiology
topic_unstemmed	misc QR1-502 misc <i<Haemagogus janthinomys</i< misc sylvatic misc arbovirus misc yellow fever misc Flaviviridae misc <i<Flavivirus</i< misc Microbiology
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title	Towards the Laboratory Maintenance of <i<Haemagogus janthinomys</i< (Dyar, 1921), the Major Neotropical Vector of Sylvatic Yellow Fever
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title_full	Towards the Laboratory Maintenance of <i<Haemagogus janthinomys</i< (Dyar, 1921), the Major Neotropical Vector of Sylvatic Yellow Fever
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author_browse	Adam Hendy Nelson Ferreira Fé Danielle Valério Eduardo Hernandez-Acosta Bárbara A. Chaves Luís Felipe Alho da Silva Rosa Amélia Gonçalves Santana Andréia da Costa Paz Matheus Mickael Mota Soares Flamarion Prado Assunção José Tenaçol Andes Chiara Andolina Vera Margarete Scarpassa Marcus Vinícius Guimarães de Lacerda Kathryn A. Hanley Nikos Vasilakis
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title_sort	towards the laboratory maintenance of <i<haemagogus janthinomys</i< (dyar, 1921), the major neotropical vector of sylvatic yellow fever
callnumber	QR1-502
title_auth	Towards the Laboratory Maintenance of <i<Haemagogus janthinomys</i< (Dyar, 1921), the Major Neotropical Vector of Sylvatic Yellow Fever
abstract	<i<Haemagogus (Haemagogus) janthinomys</i< (Dyar, 1921), the major neotropical vector of sylvatic yellow fever virus, is notoriously difficult to maintain in captivity. It has never been reared beyond an F<sub<1</sub< generation, and almost no experimental transmission studies have been performed with this species since the 1940s. Herein we describe installment hatching, artificial blood feeding, and forced-mating techniques that enabled us to produce small numbers of F<sub<3</sub< generation <i<Hg. janthinomys</i< eggs for the first time. A total of 62.8% (1562/2486) F<sub<1</sub< generation eggs hatched during ≤10 four-day cycles of immersion in a bamboo leaf infusion followed by partial drying. Hatching decreased to 20.1% (190/944) in the F<sub<2</sub< generation for eggs laid by mosquitoes copulated by forced mating. More than 85% (79/92) female F<sub<2</sub< mosquitoes fed on an artificial blood feeding system. While we were unable to maintain a laboratory colony of <i<Hg. janthinomys</i< past the F<sub<3</sub< generation, our methods provide a foundation for experimental transmission studies with this species in a laboratory setting, a critical capacity in a region with hyper-endemic transmission of dengue, Zika, and chikungunya viruses, all posing a risk of spillback into a sylvatic cycle.
abstractGer	<i<Haemagogus (Haemagogus) janthinomys</i< (Dyar, 1921), the major neotropical vector of sylvatic yellow fever virus, is notoriously difficult to maintain in captivity. It has never been reared beyond an F<sub<1</sub< generation, and almost no experimental transmission studies have been performed with this species since the 1940s. Herein we describe installment hatching, artificial blood feeding, and forced-mating techniques that enabled us to produce small numbers of F<sub<3</sub< generation <i<Hg. janthinomys</i< eggs for the first time. A total of 62.8% (1562/2486) F<sub<1</sub< generation eggs hatched during ≤10 four-day cycles of immersion in a bamboo leaf infusion followed by partial drying. Hatching decreased to 20.1% (190/944) in the F<sub<2</sub< generation for eggs laid by mosquitoes copulated by forced mating. More than 85% (79/92) female F<sub<2</sub< mosquitoes fed on an artificial blood feeding system. While we were unable to maintain a laboratory colony of <i<Hg. janthinomys</i< past the F<sub<3</sub< generation, our methods provide a foundation for experimental transmission studies with this species in a laboratory setting, a critical capacity in a region with hyper-endemic transmission of dengue, Zika, and chikungunya viruses, all posing a risk of spillback into a sylvatic cycle.
abstract_unstemmed	<i<Haemagogus (Haemagogus) janthinomys</i< (Dyar, 1921), the major neotropical vector of sylvatic yellow fever virus, is notoriously difficult to maintain in captivity. It has never been reared beyond an F<sub<1</sub< generation, and almost no experimental transmission studies have been performed with this species since the 1940s. Herein we describe installment hatching, artificial blood feeding, and forced-mating techniques that enabled us to produce small numbers of F<sub<3</sub< generation <i<Hg. janthinomys</i< eggs for the first time. A total of 62.8% (1562/2486) F<sub<1</sub< generation eggs hatched during ≤10 four-day cycles of immersion in a bamboo leaf infusion followed by partial drying. Hatching decreased to 20.1% (190/944) in the F<sub<2</sub< generation for eggs laid by mosquitoes copulated by forced mating. More than 85% (79/92) female F<sub<2</sub< mosquitoes fed on an artificial blood feeding system. While we were unable to maintain a laboratory colony of <i<Hg. janthinomys</i< past the F<sub<3</sub< generation, our methods provide a foundation for experimental transmission studies with this species in a laboratory setting, a critical capacity in a region with hyper-endemic transmission of dengue, Zika, and chikungunya viruses, all posing a risk of spillback into a sylvatic cycle.
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container_issue	1, p 45
title_short	Towards the Laboratory Maintenance of <i<Haemagogus janthinomys</i< (Dyar, 1921), the Major Neotropical Vector of Sylvatic Yellow Fever
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Towards the Laboratory Maintenance of <i<Haemagogus janthinomys</i< (Dyar, 1921), the Major Neotropical Vector of Sylvatic Yellow Fever

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Vorhandene Bände

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