Characterization of Two Porcine Parainfluenza Virus 1 Isolates and Human Parainfluenza Virus 1 Infection in Weaned Nursery Pigs

Porcine parainfluenza virus 1 (PPIV1) is a newly characterized porcine respiratory virus. Recent experimental challenge studies in three-week-old nursery pigs failed to cause disease. However, it remains unclear how genetic differences contribute to viral pathogenesis. To characterize the pathogenes...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Michael Welch [verfasserIn] Karen Krueger [verfasserIn] Jianqiang Zhang [verfasserIn] Megan Neveau [verfasserIn] Pablo Piñeyro [verfasserIn] Drew Magstadt [verfasserIn] Rodger Main [verfasserIn] Phillip Gauger [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	porcine parainfluenza virus 1 human parainfluenza virus 1 strain comparison pathogenesis

Übergeordnetes Werk:	In: Veterinary Sciences - MDPI AG, 2014, 10(2022), 1, p 18
Übergeordnetes Werk:	volume:10 ; year:2022 ; number:1, p 18

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/vetsci10010018

Katalog-ID:	DOAJ081687443

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520			\|a Porcine parainfluenza virus 1 (PPIV1) is a newly characterized porcine respiratory virus. Recent experimental challenge studies in three-week-old nursery pigs failed to cause disease. However, it remains unclear how genetic differences contribute to viral pathogenesis. To characterize the pathogenesis of different PPIV1 isolates, three-week-old nursery pigs were challenged with either PPIV1 isolate USA/MN25890NS/2016 (MN16) or USA/IA84915LG/2017 (IA17). A human parainfluenza virus 1 (HPIV1) strain C35 ATCC<sup<®</sup< VR-94™ was included to evaluate swine as a model for human parainfluenza. All viruses were successfully re-isolated from bronchoalveolar lavage fluid and detected by RT-qPCR at necropsy. Microscopic lung lesions were more severe in the IA17 group compared to the non-challenged negative control (Ctrl) group whereas differences were not found between the MN16 and Ctrl groups. Immunohistochemistry staining in respiratory samples showed a consistent trend of higher levels of PPIV1 signal in the IA17 group followed by the MN16 group, and no PPIV1 signal observed in the HPIV1 or Ctrl groups. This study suggests potential pathogenesis differences between PPIV1 isolates. Additionally, these results indicate that HPIV1 is capable of replicating in nursery pigs after experimental inoculation. However, clinical disease or gross lung lesions were not observed in any of the challenge groups.
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allfields	10.3390/vetsci10010018 doi (DE-627)DOAJ081687443 (DE-599)DOAJdcb4e6f716c542b4bd4f515275097768 DE-627 ger DE-627 rakwb eng SF600-1100 Michael Welch verfasserin aut Characterization of Two Porcine Parainfluenza Virus 1 Isolates and Human Parainfluenza Virus 1 Infection in Weaned Nursery Pigs 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Porcine parainfluenza virus 1 (PPIV1) is a newly characterized porcine respiratory virus. Recent experimental challenge studies in three-week-old nursery pigs failed to cause disease. However, it remains unclear how genetic differences contribute to viral pathogenesis. To characterize the pathogenesis of different PPIV1 isolates, three-week-old nursery pigs were challenged with either PPIV1 isolate USA/MN25890NS/2016 (MN16) or USA/IA84915LG/2017 (IA17). A human parainfluenza virus 1 (HPIV1) strain C35 ATCC<sup<®</sup< VR-94™ was included to evaluate swine as a model for human parainfluenza. All viruses were successfully re-isolated from bronchoalveolar lavage fluid and detected by RT-qPCR at necropsy. Microscopic lung lesions were more severe in the IA17 group compared to the non-challenged negative control (Ctrl) group whereas differences were not found between the MN16 and Ctrl groups. Immunohistochemistry staining in respiratory samples showed a consistent trend of higher levels of PPIV1 signal in the IA17 group followed by the MN16 group, and no PPIV1 signal observed in the HPIV1 or Ctrl groups. This study suggests potential pathogenesis differences between PPIV1 isolates. Additionally, these results indicate that HPIV1 is capable of replicating in nursery pigs after experimental inoculation. However, clinical disease or gross lung lesions were not observed in any of the challenge groups. porcine parainfluenza virus 1 human parainfluenza virus 1 <i<Paramyxoviridae</i< <i<Respirovirus</i< strain comparison pathogenesis Veterinary medicine Karen Krueger verfasserin aut Jianqiang Zhang verfasserin aut Megan Neveau verfasserin aut Pablo Piñeyro verfasserin aut Drew Magstadt verfasserin aut Rodger Main verfasserin aut Phillip Gauger verfasserin aut In Veterinary Sciences MDPI AG, 2014 10(2022), 1, p 18 (DE-627)785698477 (DE-600)2768971-2 23067381 nnns volume:10 year:2022 number:1, p 18 https://doi.org/10.3390/vetsci10010018 kostenfrei https://doaj.org/article/dcb4e6f716c542b4bd4f515275097768 kostenfrei https://www.mdpi.com/2306-7381/10/1/18 kostenfrei https://doaj.org/toc/2306-7381 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2446 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2022 1, p 18
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allfieldsGer	10.3390/vetsci10010018 doi (DE-627)DOAJ081687443 (DE-599)DOAJdcb4e6f716c542b4bd4f515275097768 DE-627 ger DE-627 rakwb eng SF600-1100 Michael Welch verfasserin aut Characterization of Two Porcine Parainfluenza Virus 1 Isolates and Human Parainfluenza Virus 1 Infection in Weaned Nursery Pigs 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Porcine parainfluenza virus 1 (PPIV1) is a newly characterized porcine respiratory virus. Recent experimental challenge studies in three-week-old nursery pigs failed to cause disease. However, it remains unclear how genetic differences contribute to viral pathogenesis. To characterize the pathogenesis of different PPIV1 isolates, three-week-old nursery pigs were challenged with either PPIV1 isolate USA/MN25890NS/2016 (MN16) or USA/IA84915LG/2017 (IA17). A human parainfluenza virus 1 (HPIV1) strain C35 ATCC<sup<®</sup< VR-94™ was included to evaluate swine as a model for human parainfluenza. All viruses were successfully re-isolated from bronchoalveolar lavage fluid and detected by RT-qPCR at necropsy. Microscopic lung lesions were more severe in the IA17 group compared to the non-challenged negative control (Ctrl) group whereas differences were not found between the MN16 and Ctrl groups. Immunohistochemistry staining in respiratory samples showed a consistent trend of higher levels of PPIV1 signal in the IA17 group followed by the MN16 group, and no PPIV1 signal observed in the HPIV1 or Ctrl groups. This study suggests potential pathogenesis differences between PPIV1 isolates. Additionally, these results indicate that HPIV1 is capable of replicating in nursery pigs after experimental inoculation. However, clinical disease or gross lung lesions were not observed in any of the challenge groups. porcine parainfluenza virus 1 human parainfluenza virus 1 <i<Paramyxoviridae</i< <i<Respirovirus</i< strain comparison pathogenesis Veterinary medicine Karen Krueger verfasserin aut Jianqiang Zhang verfasserin aut Megan Neveau verfasserin aut Pablo Piñeyro verfasserin aut Drew Magstadt verfasserin aut Rodger Main verfasserin aut Phillip Gauger verfasserin aut In Veterinary Sciences MDPI AG, 2014 10(2022), 1, p 18 (DE-627)785698477 (DE-600)2768971-2 23067381 nnns volume:10 year:2022 number:1, p 18 https://doi.org/10.3390/vetsci10010018 kostenfrei https://doaj.org/article/dcb4e6f716c542b4bd4f515275097768 kostenfrei https://www.mdpi.com/2306-7381/10/1/18 kostenfrei https://doaj.org/toc/2306-7381 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2446 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2022 1, p 18
allfieldsSound	10.3390/vetsci10010018 doi (DE-627)DOAJ081687443 (DE-599)DOAJdcb4e6f716c542b4bd4f515275097768 DE-627 ger DE-627 rakwb eng SF600-1100 Michael Welch verfasserin aut Characterization of Two Porcine Parainfluenza Virus 1 Isolates and Human Parainfluenza Virus 1 Infection in Weaned Nursery Pigs 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Porcine parainfluenza virus 1 (PPIV1) is a newly characterized porcine respiratory virus. Recent experimental challenge studies in three-week-old nursery pigs failed to cause disease. However, it remains unclear how genetic differences contribute to viral pathogenesis. To characterize the pathogenesis of different PPIV1 isolates, three-week-old nursery pigs were challenged with either PPIV1 isolate USA/MN25890NS/2016 (MN16) or USA/IA84915LG/2017 (IA17). A human parainfluenza virus 1 (HPIV1) strain C35 ATCC<sup<®</sup< VR-94™ was included to evaluate swine as a model for human parainfluenza. All viruses were successfully re-isolated from bronchoalveolar lavage fluid and detected by RT-qPCR at necropsy. Microscopic lung lesions were more severe in the IA17 group compared to the non-challenged negative control (Ctrl) group whereas differences were not found between the MN16 and Ctrl groups. Immunohistochemistry staining in respiratory samples showed a consistent trend of higher levels of PPIV1 signal in the IA17 group followed by the MN16 group, and no PPIV1 signal observed in the HPIV1 or Ctrl groups. This study suggests potential pathogenesis differences between PPIV1 isolates. Additionally, these results indicate that HPIV1 is capable of replicating in nursery pigs after experimental inoculation. However, clinical disease or gross lung lesions were not observed in any of the challenge groups. porcine parainfluenza virus 1 human parainfluenza virus 1 <i<Paramyxoviridae</i< <i<Respirovirus</i< strain comparison pathogenesis Veterinary medicine Karen Krueger verfasserin aut Jianqiang Zhang verfasserin aut Megan Neveau verfasserin aut Pablo Piñeyro verfasserin aut Drew Magstadt verfasserin aut Rodger Main verfasserin aut Phillip Gauger verfasserin aut In Veterinary Sciences MDPI AG, 2014 10(2022), 1, p 18 (DE-627)785698477 (DE-600)2768971-2 23067381 nnns volume:10 year:2022 number:1, p 18 https://doi.org/10.3390/vetsci10010018 kostenfrei https://doaj.org/article/dcb4e6f716c542b4bd4f515275097768 kostenfrei https://www.mdpi.com/2306-7381/10/1/18 kostenfrei https://doaj.org/toc/2306-7381 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2446 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2022 1, p 18
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callnumber-first	S - Agriculture
author	Michael Welch
spellingShingle	Michael Welch misc SF600-1100 misc porcine parainfluenza virus 1 misc human parainfluenza virus 1 misc <i<Paramyxoviridae</i< misc <i<Respirovirus</i< misc strain comparison misc pathogenesis misc Veterinary medicine Characterization of Two Porcine Parainfluenza Virus 1 Isolates and Human Parainfluenza Virus 1 Infection in Weaned Nursery Pigs
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topic_title	SF600-1100 Characterization of Two Porcine Parainfluenza Virus 1 Isolates and Human Parainfluenza Virus 1 Infection in Weaned Nursery Pigs porcine parainfluenza virus 1 human parainfluenza virus 1 <i<Paramyxoviridae</i< <i<Respirovirus</i< strain comparison pathogenesis
topic	misc SF600-1100 misc porcine parainfluenza virus 1 misc human parainfluenza virus 1 misc <i<Paramyxoviridae</i< misc <i<Respirovirus</i< misc strain comparison misc pathogenesis misc Veterinary medicine
topic_unstemmed	misc SF600-1100 misc porcine parainfluenza virus 1 misc human parainfluenza virus 1 misc <i<Paramyxoviridae</i< misc <i<Respirovirus</i< misc strain comparison misc pathogenesis misc Veterinary medicine
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title	Characterization of Two Porcine Parainfluenza Virus 1 Isolates and Human Parainfluenza Virus 1 Infection in Weaned Nursery Pigs
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title_full	Characterization of Two Porcine Parainfluenza Virus 1 Isolates and Human Parainfluenza Virus 1 Infection in Weaned Nursery Pigs
author_sort	Michael Welch
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author_browse	Michael Welch Karen Krueger Jianqiang Zhang Megan Neveau Pablo Piñeyro Drew Magstadt Rodger Main Phillip Gauger
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title_sort	characterization of two porcine parainfluenza virus 1 isolates and human parainfluenza virus 1 infection in weaned nursery pigs
callnumber	SF600-1100
title_auth	Characterization of Two Porcine Parainfluenza Virus 1 Isolates and Human Parainfluenza Virus 1 Infection in Weaned Nursery Pigs
abstract	Porcine parainfluenza virus 1 (PPIV1) is a newly characterized porcine respiratory virus. Recent experimental challenge studies in three-week-old nursery pigs failed to cause disease. However, it remains unclear how genetic differences contribute to viral pathogenesis. To characterize the pathogenesis of different PPIV1 isolates, three-week-old nursery pigs were challenged with either PPIV1 isolate USA/MN25890NS/2016 (MN16) or USA/IA84915LG/2017 (IA17). A human parainfluenza virus 1 (HPIV1) strain C35 ATCC<sup<®</sup< VR-94™ was included to evaluate swine as a model for human parainfluenza. All viruses were successfully re-isolated from bronchoalveolar lavage fluid and detected by RT-qPCR at necropsy. Microscopic lung lesions were more severe in the IA17 group compared to the non-challenged negative control (Ctrl) group whereas differences were not found between the MN16 and Ctrl groups. Immunohistochemistry staining in respiratory samples showed a consistent trend of higher levels of PPIV1 signal in the IA17 group followed by the MN16 group, and no PPIV1 signal observed in the HPIV1 or Ctrl groups. This study suggests potential pathogenesis differences between PPIV1 isolates. Additionally, these results indicate that HPIV1 is capable of replicating in nursery pigs after experimental inoculation. However, clinical disease or gross lung lesions were not observed in any of the challenge groups.
abstractGer	Porcine parainfluenza virus 1 (PPIV1) is a newly characterized porcine respiratory virus. Recent experimental challenge studies in three-week-old nursery pigs failed to cause disease. However, it remains unclear how genetic differences contribute to viral pathogenesis. To characterize the pathogenesis of different PPIV1 isolates, three-week-old nursery pigs were challenged with either PPIV1 isolate USA/MN25890NS/2016 (MN16) or USA/IA84915LG/2017 (IA17). A human parainfluenza virus 1 (HPIV1) strain C35 ATCC<sup<®</sup< VR-94™ was included to evaluate swine as a model for human parainfluenza. All viruses were successfully re-isolated from bronchoalveolar lavage fluid and detected by RT-qPCR at necropsy. Microscopic lung lesions were more severe in the IA17 group compared to the non-challenged negative control (Ctrl) group whereas differences were not found between the MN16 and Ctrl groups. Immunohistochemistry staining in respiratory samples showed a consistent trend of higher levels of PPIV1 signal in the IA17 group followed by the MN16 group, and no PPIV1 signal observed in the HPIV1 or Ctrl groups. This study suggests potential pathogenesis differences between PPIV1 isolates. Additionally, these results indicate that HPIV1 is capable of replicating in nursery pigs after experimental inoculation. However, clinical disease or gross lung lesions were not observed in any of the challenge groups.
abstract_unstemmed	Porcine parainfluenza virus 1 (PPIV1) is a newly characterized porcine respiratory virus. Recent experimental challenge studies in three-week-old nursery pigs failed to cause disease. However, it remains unclear how genetic differences contribute to viral pathogenesis. To characterize the pathogenesis of different PPIV1 isolates, three-week-old nursery pigs were challenged with either PPIV1 isolate USA/MN25890NS/2016 (MN16) or USA/IA84915LG/2017 (IA17). A human parainfluenza virus 1 (HPIV1) strain C35 ATCC<sup<®</sup< VR-94™ was included to evaluate swine as a model for human parainfluenza. All viruses were successfully re-isolated from bronchoalveolar lavage fluid and detected by RT-qPCR at necropsy. Microscopic lung lesions were more severe in the IA17 group compared to the non-challenged negative control (Ctrl) group whereas differences were not found between the MN16 and Ctrl groups. Immunohistochemistry staining in respiratory samples showed a consistent trend of higher levels of PPIV1 signal in the IA17 group followed by the MN16 group, and no PPIV1 signal observed in the HPIV1 or Ctrl groups. This study suggests potential pathogenesis differences between PPIV1 isolates. Additionally, these results indicate that HPIV1 is capable of replicating in nursery pigs after experimental inoculation. However, clinical disease or gross lung lesions were not observed in any of the challenge groups.
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title_short	Characterization of Two Porcine Parainfluenza Virus 1 Isolates and Human Parainfluenza Virus 1 Infection in Weaned Nursery Pigs
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author2	Karen Krueger Jianqiang Zhang Megan Neveau Pablo Piñeyro Drew Magstadt Rodger Main Phillip Gauger
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Characterization of Two Porcine Parainfluenza Virus 1 Isolates and Human Parainfluenza Virus 1 Infection in Weaned Nursery Pigs

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