Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells

Tks4 is a large scaffold protein in the EGFR signal transduction pathway that is involved in several cellular processes, such as cellular motility, reactive oxygen species-dependent processes, and embryonic development. It is also implicated in a rare developmental disorder, Frank–ter Haar syndrome....
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Autor*in:	Mevan Jacksi [verfasserIn] Eva Schad [verfasserIn] László Buday [verfasserIn] Agnes Tantos [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	Tks4 signal transduction long non-coding RNA gene regulation EGFR signaling epithelial–mesenchymal transition

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 24(2023), 2, p 1310
Übergeordnetes Werk:	volume:24 ; year:2023 ; number:2, p 1310

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms24021310

Katalog-ID:	DOAJ08178158X

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520			\|a Tks4 is a large scaffold protein in the EGFR signal transduction pathway that is involved in several cellular processes, such as cellular motility, reactive oxygen species-dependent processes, and embryonic development. It is also implicated in a rare developmental disorder, Frank–ter Haar syndrome. Loss of Tks4 resulted in the induction of an EMT-like process, with increased motility and overexpression of EMT markers in colorectal carcinoma cells. In this work, we explored the broader effects of deletion of Tks4 on the gene expression pattern of HCT116 colorectal carcinoma cells by transcriptome sequencing of wild-type and Tks4 knockout (KO) cells. We identified several protein coding genes with altered mRNA levels in the Tks4 KO cell line, as well as a set of long non-coding RNAs, and confirmed these changes with quantitative PCR on a selected set of genes. Our results show a significant perturbation of gene expression upon the deletion of Tks4, suggesting the involvement of different signal transduction pathways over the well-known EGFR signaling.
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spelling	10.3390/ijms24021310 doi (DE-627)DOAJ08178158X (DE-599)DOAJ06ce6e025d9545aab2f5875c2ad9d61e DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Mevan Jacksi verfasserin aut Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Tks4 is a large scaffold protein in the EGFR signal transduction pathway that is involved in several cellular processes, such as cellular motility, reactive oxygen species-dependent processes, and embryonic development. It is also implicated in a rare developmental disorder, Frank–ter Haar syndrome. Loss of Tks4 resulted in the induction of an EMT-like process, with increased motility and overexpression of EMT markers in colorectal carcinoma cells. In this work, we explored the broader effects of deletion of Tks4 on the gene expression pattern of HCT116 colorectal carcinoma cells by transcriptome sequencing of wild-type and Tks4 knockout (KO) cells. We identified several protein coding genes with altered mRNA levels in the Tks4 KO cell line, as well as a set of long non-coding RNAs, and confirmed these changes with quantitative PCR on a selected set of genes. Our results show a significant perturbation of gene expression upon the deletion of Tks4, suggesting the involvement of different signal transduction pathways over the well-known EGFR signaling. Tks4 signal transduction long non-coding RNA gene regulation EGFR signaling epithelial–mesenchymal transition Biology (General) Chemistry Eva Schad verfasserin aut László Buday verfasserin aut Agnes Tantos verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 2, p 1310 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:2, p 1310 https://doi.org/10.3390/ijms24021310 kostenfrei https://doaj.org/article/06ce6e025d9545aab2f5875c2ad9d61e kostenfrei https://www.mdpi.com/1422-0067/24/2/1310 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 2, p 1310
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allfieldsSound	10.3390/ijms24021310 doi (DE-627)DOAJ08178158X (DE-599)DOAJ06ce6e025d9545aab2f5875c2ad9d61e DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Mevan Jacksi verfasserin aut Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Tks4 is a large scaffold protein in the EGFR signal transduction pathway that is involved in several cellular processes, such as cellular motility, reactive oxygen species-dependent processes, and embryonic development. It is also implicated in a rare developmental disorder, Frank–ter Haar syndrome. Loss of Tks4 resulted in the induction of an EMT-like process, with increased motility and overexpression of EMT markers in colorectal carcinoma cells. In this work, we explored the broader effects of deletion of Tks4 on the gene expression pattern of HCT116 colorectal carcinoma cells by transcriptome sequencing of wild-type and Tks4 knockout (KO) cells. We identified several protein coding genes with altered mRNA levels in the Tks4 KO cell line, as well as a set of long non-coding RNAs, and confirmed these changes with quantitative PCR on a selected set of genes. Our results show a significant perturbation of gene expression upon the deletion of Tks4, suggesting the involvement of different signal transduction pathways over the well-known EGFR signaling. Tks4 signal transduction long non-coding RNA gene regulation EGFR signaling epithelial–mesenchymal transition Biology (General) Chemistry Eva Schad verfasserin aut László Buday verfasserin aut Agnes Tantos verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 2, p 1310 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:2, p 1310 https://doi.org/10.3390/ijms24021310 kostenfrei https://doaj.org/article/06ce6e025d9545aab2f5875c2ad9d61e kostenfrei https://www.mdpi.com/1422-0067/24/2/1310 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 2, p 1310
language	English
source	In International Journal of Molecular Sciences 24(2023), 2, p 1310 volume:24 year:2023 number:2, p 1310
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topic_facet	Tks4 signal transduction long non-coding RNA gene regulation EGFR signaling epithelial–mesenchymal transition Biology (General) Chemistry
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authorswithroles_txt_mv	Mevan Jacksi @@aut@@ Eva Schad @@aut@@ László Buday @@aut@@ Agnes Tantos @@aut@@
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callnumber-first	Q - Science
author	Mevan Jacksi
spellingShingle	Mevan Jacksi misc QH301-705.5 misc QD1-999 misc Tks4 misc signal transduction misc long non-coding RNA misc gene regulation misc EGFR signaling misc epithelial–mesenchymal transition misc Biology (General) misc Chemistry Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells
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topic_title	QH301-705.5 QD1-999 Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells Tks4 signal transduction long non-coding RNA gene regulation EGFR signaling epithelial–mesenchymal transition
topic	misc QH301-705.5 misc QD1-999 misc Tks4 misc signal transduction misc long non-coding RNA misc gene regulation misc EGFR signaling misc epithelial–mesenchymal transition misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc Tks4 misc signal transduction misc long non-coding RNA misc gene regulation misc EGFR signaling misc epithelial–mesenchymal transition misc Biology (General) misc Chemistry
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title	Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells
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title_full	Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells
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title_sort	absence of scaffold protein tks4 disrupts several signaling pathways in colon cancer cells
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title_auth	Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells
abstract	Tks4 is a large scaffold protein in the EGFR signal transduction pathway that is involved in several cellular processes, such as cellular motility, reactive oxygen species-dependent processes, and embryonic development. It is also implicated in a rare developmental disorder, Frank–ter Haar syndrome. Loss of Tks4 resulted in the induction of an EMT-like process, with increased motility and overexpression of EMT markers in colorectal carcinoma cells. In this work, we explored the broader effects of deletion of Tks4 on the gene expression pattern of HCT116 colorectal carcinoma cells by transcriptome sequencing of wild-type and Tks4 knockout (KO) cells. We identified several protein coding genes with altered mRNA levels in the Tks4 KO cell line, as well as a set of long non-coding RNAs, and confirmed these changes with quantitative PCR on a selected set of genes. Our results show a significant perturbation of gene expression upon the deletion of Tks4, suggesting the involvement of different signal transduction pathways over the well-known EGFR signaling.
abstractGer	Tks4 is a large scaffold protein in the EGFR signal transduction pathway that is involved in several cellular processes, such as cellular motility, reactive oxygen species-dependent processes, and embryonic development. It is also implicated in a rare developmental disorder, Frank–ter Haar syndrome. Loss of Tks4 resulted in the induction of an EMT-like process, with increased motility and overexpression of EMT markers in colorectal carcinoma cells. In this work, we explored the broader effects of deletion of Tks4 on the gene expression pattern of HCT116 colorectal carcinoma cells by transcriptome sequencing of wild-type and Tks4 knockout (KO) cells. We identified several protein coding genes with altered mRNA levels in the Tks4 KO cell line, as well as a set of long non-coding RNAs, and confirmed these changes with quantitative PCR on a selected set of genes. Our results show a significant perturbation of gene expression upon the deletion of Tks4, suggesting the involvement of different signal transduction pathways over the well-known EGFR signaling.
abstract_unstemmed	Tks4 is a large scaffold protein in the EGFR signal transduction pathway that is involved in several cellular processes, such as cellular motility, reactive oxygen species-dependent processes, and embryonic development. It is also implicated in a rare developmental disorder, Frank–ter Haar syndrome. Loss of Tks4 resulted in the induction of an EMT-like process, with increased motility and overexpression of EMT markers in colorectal carcinoma cells. In this work, we explored the broader effects of deletion of Tks4 on the gene expression pattern of HCT116 colorectal carcinoma cells by transcriptome sequencing of wild-type and Tks4 knockout (KO) cells. We identified several protein coding genes with altered mRNA levels in the Tks4 KO cell line, as well as a set of long non-coding RNAs, and confirmed these changes with quantitative PCR on a selected set of genes. Our results show a significant perturbation of gene expression upon the deletion of Tks4, suggesting the involvement of different signal transduction pathways over the well-known EGFR signaling.
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title_short	Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells
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