A Two-Compartment Fermentation System to Quantify Strain-Specific Interactions in Microbial Co-Cultures

To fulfil the growing interest in investigating microbial interactions in co-cultures, a novel two-compartment bioreactor system was developed, characterised, and implemented. The system allowed for the exchange of amino acids and peptides via a polyethersulfone membrane that retained biomass. Furth...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Andreas Ulmer [verfasserIn] Stefan Veit [verfasserIn] Florian Erdemann [verfasserIn] Andreas Freund [verfasserIn] Maren Loesch [verfasserIn] Attila Teleki [verfasserIn] Ahmad A. Zeidan [verfasserIn] Ralf Takors [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	microbial consortia metabolomics lactic acid bacteria bioprocess engineering

Übergeordnetes Werk:	In: Bioengineering - MDPI AG, 2014, 10(2023), 1, p 103
Übergeordnetes Werk:	volume:10 ; year:2023 ; number:1, p 103

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/bioengineering10010103

Katalog-ID:	DOAJ081846762

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topic_title	QH301-705.5 A Two-Compartment Fermentation System to Quantify Strain-Specific Interactions in Microbial Co-Cultures microbial consortia metabolomics lactic acid bacteria <i<Streptococcus thermophilus</i< <i<Lactobacillus bulgaricus</i< bioprocess engineering
topic	misc QH301-705.5 misc microbial consortia misc metabolomics misc lactic acid bacteria misc <i<Streptococcus thermophilus</i< misc <i<Lactobacillus bulgaricus</i< misc bioprocess engineering misc Technology misc T misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc microbial consortia misc metabolomics misc lactic acid bacteria misc <i<Streptococcus thermophilus</i< misc <i<Lactobacillus bulgaricus</i< misc bioprocess engineering misc Technology misc T misc Biology (General)
topic_browse	misc QH301-705.5 misc microbial consortia misc metabolomics misc lactic acid bacteria misc <i<Streptococcus thermophilus</i< misc <i<Lactobacillus bulgaricus</i< misc bioprocess engineering misc Technology misc T misc Biology (General)
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title	A Two-Compartment Fermentation System to Quantify Strain-Specific Interactions in Microbial Co-Cultures
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title_full	A Two-Compartment Fermentation System to Quantify Strain-Specific Interactions in Microbial Co-Cultures
author_sort	Andreas Ulmer
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author_browse	Andreas Ulmer Stefan Veit Florian Erdemann Andreas Freund Maren Loesch Attila Teleki Ahmad A. Zeidan Ralf Takors
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doi_str_mv	10.3390/bioengineering10010103
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title_sort	two-compartment fermentation system to quantify strain-specific interactions in microbial co-cultures
callnumber	QH301-705.5
title_auth	A Two-Compartment Fermentation System to Quantify Strain-Specific Interactions in Microbial Co-Cultures
abstract	To fulfil the growing interest in investigating microbial interactions in co-cultures, a novel two-compartment bioreactor system was developed, characterised, and implemented. The system allowed for the exchange of amino acids and peptides via a polyethersulfone membrane that retained biomass. Further system characterisation revealed a Bodenstein number of 18, which hints at backmixing. Together with other physical settings, the existence of unwanted inner-compartment substrate gradients could be ruled out. Furthermore, the study of Damkoehler numbers indicated that a proper metabolite supply between compartments was enabled. Implementing the two-compartment system (2cs) for growing <i<Streptococcus thermophilus</i< and <i<Lactobacillus delbrueckii</i< subs. <i<bulgaricus</i<, which are microorganisms commonly used in yogurt starter cultures, revealed only a small variance between the one-compartment and two-compartment approaches. The 2cs enabled the quantification of the strain-specific production and consumption rates of amino acids in an interacting <i<S. thermophilus</i<–<i<L. bulgaricus</i< co-culture. Therefore, comparisons between mono- and co-culture performance could be achieved. Both species produce and release amino acids. Only alanine was produced <i<de novo</i< from glucose through potential transaminase activity by <i<L. bulgaricus</i< and consumed by <i<S. thermophilus</i<. Arginine availability in peptides was limited to <i<S. thermophilus’</i< growth, indicating active biosynthesis and dependency on the proteolytic activity of <i<L. bulgaricus</i<. The application of the 2cs not only opens the door for the quantification of exchange fluxes between microbes but also enables continuous production modes, for example, for targeted evolution studies.
abstractGer	To fulfil the growing interest in investigating microbial interactions in co-cultures, a novel two-compartment bioreactor system was developed, characterised, and implemented. The system allowed for the exchange of amino acids and peptides via a polyethersulfone membrane that retained biomass. Further system characterisation revealed a Bodenstein number of 18, which hints at backmixing. Together with other physical settings, the existence of unwanted inner-compartment substrate gradients could be ruled out. Furthermore, the study of Damkoehler numbers indicated that a proper metabolite supply between compartments was enabled. Implementing the two-compartment system (2cs) for growing <i<Streptococcus thermophilus</i< and <i<Lactobacillus delbrueckii</i< subs. <i<bulgaricus</i<, which are microorganisms commonly used in yogurt starter cultures, revealed only a small variance between the one-compartment and two-compartment approaches. The 2cs enabled the quantification of the strain-specific production and consumption rates of amino acids in an interacting <i<S. thermophilus</i<–<i<L. bulgaricus</i< co-culture. Therefore, comparisons between mono- and co-culture performance could be achieved. Both species produce and release amino acids. Only alanine was produced <i<de novo</i< from glucose through potential transaminase activity by <i<L. bulgaricus</i< and consumed by <i<S. thermophilus</i<. Arginine availability in peptides was limited to <i<S. thermophilus’</i< growth, indicating active biosynthesis and dependency on the proteolytic activity of <i<L. bulgaricus</i<. The application of the 2cs not only opens the door for the quantification of exchange fluxes between microbes but also enables continuous production modes, for example, for targeted evolution studies.
abstract_unstemmed	To fulfil the growing interest in investigating microbial interactions in co-cultures, a novel two-compartment bioreactor system was developed, characterised, and implemented. The system allowed for the exchange of amino acids and peptides via a polyethersulfone membrane that retained biomass. Further system characterisation revealed a Bodenstein number of 18, which hints at backmixing. Together with other physical settings, the existence of unwanted inner-compartment substrate gradients could be ruled out. Furthermore, the study of Damkoehler numbers indicated that a proper metabolite supply between compartments was enabled. Implementing the two-compartment system (2cs) for growing <i<Streptococcus thermophilus</i< and <i<Lactobacillus delbrueckii</i< subs. <i<bulgaricus</i<, which are microorganisms commonly used in yogurt starter cultures, revealed only a small variance between the one-compartment and two-compartment approaches. The 2cs enabled the quantification of the strain-specific production and consumption rates of amino acids in an interacting <i<S. thermophilus</i<–<i<L. bulgaricus</i< co-culture. Therefore, comparisons between mono- and co-culture performance could be achieved. Both species produce and release amino acids. Only alanine was produced <i<de novo</i< from glucose through potential transaminase activity by <i<L. bulgaricus</i< and consumed by <i<S. thermophilus</i<. Arginine availability in peptides was limited to <i<S. thermophilus’</i< growth, indicating active biosynthesis and dependency on the proteolytic activity of <i<L. bulgaricus</i<. The application of the 2cs not only opens the door for the quantification of exchange fluxes between microbes but also enables continuous production modes, for example, for targeted evolution studies.
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title_short	A Two-Compartment Fermentation System to Quantify Strain-Specific Interactions in Microbial Co-Cultures
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author2	Stefan Veit Florian Erdemann Andreas Freund Maren Loesch Attila Teleki Ahmad A. Zeidan Ralf Takors
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