Plant regeneration from in vitro leaves of four commercial Pyrus species

An efficient shoot regeneration from in vitro leaf sections of Pyrus communis Bartlett, P. pyrifolia Shenbuzhi, P. bretschneideri Zaosu and P. ussuriensis Manyuanxiang was successfully developed for use in future transgenic studies. On the basis of regeneration frequency and average shoot numbers, o...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	H. Tang [verfasserIn] Y. Luo [verfasserIn] C. Liu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2008

Schlagwörter:	keywords: adventitious shoot leaf explant organogenesis pear caulogenesis pyrus

Übergeordnetes Werk:	In: Plant, Soil and Environment - Czech Academy of Agricultural Sciences, 2010, 54(2008), 4, Seite 140-148
Übergeordnetes Werk:	volume:54 ; year:2008 ; number:4 ; pages:140-148

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.17221/389-PSE

Katalog-ID:	DOAJ082832439

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520			\|a An efficient shoot regeneration from in vitro leaf sections of Pyrus communis Bartlett, P. pyrifolia Shenbuzhi, P. bretschneideri Zaosu and P. ussuriensis Manyuanxiang was successfully developed for use in future transgenic studies. On the basis of regeneration frequency and average shoot numbers, optimal shoot regeneration was obtained on leaf sections of P. communis Bartlett when cultured on Murashige and Skoog complete medium containing 6.0 mg/l BA (6-benzyladenine) and 0.1 mg/l NAA (α-naphthaleneacetic acid), while Quoirin and Lepoivre complete medium supplemented with 1.0 mg/l TDZ [thidiazuron (N-phenyl-N1-1,2,3-thiadiazol-5-ylurea)] and 0.1 mg/l NAA was found best for P. pyrifolia Shenbuzhi, and Nitsch and Nitsch complete medium containing 3.0 mg/l TDZ and 0.1 mg/l NAA or 0.2 mg/l IAA was suitable forP. bretschneideri Zaosu or P. ussuriensis Manyuanxiang, respectively. After cutting the leaves into three sections perpendicular to the midrib and culturing under the equivalent conditions, regeneration occurred more frequently on basal sections than middle sections, and no shoots formed on apical sections. A ratio of NH+4-N/NO-3- N of 1:2~1:7 was found beneficial for shoot regeneration. 75.0-87.5% of proliferating shoots formed roots after 4 weeks of transfer to 1/4 strength of Murashige and Skoog complete medium supplemented with 2.5 mg/l IBA (indole-3-butryric acid) and 30.0 g/l sucrose. Regenerated plants were successfully established under greenhouse conditions.
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matchkey_str	article:18059368:2008----::lnrgnrtofoivtoevsforom
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allfields	10.17221/389-PSE doi (DE-627)DOAJ082832439 (DE-599)DOAJ52ecfa391d4542a9863d4021c17fdb78 DE-627 ger DE-627 rakwb eng SB1-1110 H. Tang verfasserin aut Plant regeneration from in vitro leaves of four commercial Pyrus species 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier An efficient shoot regeneration from in vitro leaf sections of Pyrus communis Bartlett, P. pyrifolia Shenbuzhi, P. bretschneideri Zaosu and P. ussuriensis Manyuanxiang was successfully developed for use in future transgenic studies. On the basis of regeneration frequency and average shoot numbers, optimal shoot regeneration was obtained on leaf sections of P. communis Bartlett when cultured on Murashige and Skoog complete medium containing 6.0 mg/l BA (6-benzyladenine) and 0.1 mg/l NAA (α-naphthaleneacetic acid), while Quoirin and Lepoivre complete medium supplemented with 1.0 mg/l TDZ [thidiazuron (N-phenyl-N1-1,2,3-thiadiazol-5-ylurea)] and 0.1 mg/l NAA was found best for P. pyrifolia Shenbuzhi, and Nitsch and Nitsch complete medium containing 3.0 mg/l TDZ and 0.1 mg/l NAA or 0.2 mg/l IAA was suitable forP. bretschneideri Zaosu or P. ussuriensis Manyuanxiang, respectively. After cutting the leaves into three sections perpendicular to the midrib and culturing under the equivalent conditions, regeneration occurred more frequently on basal sections than middle sections, and no shoots formed on apical sections. A ratio of NH+4-N/NO-3- N of 1:2~1:7 was found beneficial for shoot regeneration. 75.0-87.5% of proliferating shoots formed roots after 4 weeks of transfer to 1/4 strength of Murashige and Skoog complete medium supplemented with 2.5 mg/l IBA (indole-3-butryric acid) and 30.0 g/l sucrose. Regenerated plants were successfully established under greenhouse conditions. keywords: adventitious shoot leaf explant organogenesis pear caulogenesis pyrus Plant culture Y. Luo verfasserin aut C. Liu verfasserin aut In Plant, Soil and Environment Czech Academy of Agricultural Sciences, 2010 54(2008), 4, Seite 140-148 (DE-627)387477543 (DE-600)2145229-5 18059368 nnns volume:54 year:2008 number:4 pages:140-148 https://doi.org/10.17221/389-PSE kostenfrei https://doaj.org/article/52ecfa391d4542a9863d4021c17fdb78 kostenfrei https://pse.agriculturejournals.cz/artkey/pse-200804-0002_plant-regeneration-from-in-vitro-leaves-of-four-commercial-pyrus-species.php kostenfrei https://doaj.org/toc/1214-1178 Journal toc kostenfrei https://doaj.org/toc/1805-9368 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 54 2008 4 140-148
spelling	10.17221/389-PSE doi (DE-627)DOAJ082832439 (DE-599)DOAJ52ecfa391d4542a9863d4021c17fdb78 DE-627 ger DE-627 rakwb eng SB1-1110 H. Tang verfasserin aut Plant regeneration from in vitro leaves of four commercial Pyrus species 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier An efficient shoot regeneration from in vitro leaf sections of Pyrus communis Bartlett, P. pyrifolia Shenbuzhi, P. bretschneideri Zaosu and P. ussuriensis Manyuanxiang was successfully developed for use in future transgenic studies. On the basis of regeneration frequency and average shoot numbers, optimal shoot regeneration was obtained on leaf sections of P. communis Bartlett when cultured on Murashige and Skoog complete medium containing 6.0 mg/l BA (6-benzyladenine) and 0.1 mg/l NAA (α-naphthaleneacetic acid), while Quoirin and Lepoivre complete medium supplemented with 1.0 mg/l TDZ [thidiazuron (N-phenyl-N1-1,2,3-thiadiazol-5-ylurea)] and 0.1 mg/l NAA was found best for P. pyrifolia Shenbuzhi, and Nitsch and Nitsch complete medium containing 3.0 mg/l TDZ and 0.1 mg/l NAA or 0.2 mg/l IAA was suitable forP. bretschneideri Zaosu or P. ussuriensis Manyuanxiang, respectively. After cutting the leaves into three sections perpendicular to the midrib and culturing under the equivalent conditions, regeneration occurred more frequently on basal sections than middle sections, and no shoots formed on apical sections. A ratio of NH+4-N/NO-3- N of 1:2~1:7 was found beneficial for shoot regeneration. 75.0-87.5% of proliferating shoots formed roots after 4 weeks of transfer to 1/4 strength of Murashige and Skoog complete medium supplemented with 2.5 mg/l IBA (indole-3-butryric acid) and 30.0 g/l sucrose. Regenerated plants were successfully established under greenhouse conditions. keywords: adventitious shoot leaf explant organogenesis pear caulogenesis pyrus Plant culture Y. Luo verfasserin aut C. Liu verfasserin aut In Plant, Soil and Environment Czech Academy of Agricultural Sciences, 2010 54(2008), 4, Seite 140-148 (DE-627)387477543 (DE-600)2145229-5 18059368 nnns volume:54 year:2008 number:4 pages:140-148 https://doi.org/10.17221/389-PSE kostenfrei https://doaj.org/article/52ecfa391d4542a9863d4021c17fdb78 kostenfrei https://pse.agriculturejournals.cz/artkey/pse-200804-0002_plant-regeneration-from-in-vitro-leaves-of-four-commercial-pyrus-species.php kostenfrei https://doaj.org/toc/1214-1178 Journal toc kostenfrei https://doaj.org/toc/1805-9368 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 54 2008 4 140-148
allfields_unstemmed	10.17221/389-PSE doi (DE-627)DOAJ082832439 (DE-599)DOAJ52ecfa391d4542a9863d4021c17fdb78 DE-627 ger DE-627 rakwb eng SB1-1110 H. Tang verfasserin aut Plant regeneration from in vitro leaves of four commercial Pyrus species 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier An efficient shoot regeneration from in vitro leaf sections of Pyrus communis Bartlett, P. pyrifolia Shenbuzhi, P. bretschneideri Zaosu and P. ussuriensis Manyuanxiang was successfully developed for use in future transgenic studies. On the basis of regeneration frequency and average shoot numbers, optimal shoot regeneration was obtained on leaf sections of P. communis Bartlett when cultured on Murashige and Skoog complete medium containing 6.0 mg/l BA (6-benzyladenine) and 0.1 mg/l NAA (α-naphthaleneacetic acid), while Quoirin and Lepoivre complete medium supplemented with 1.0 mg/l TDZ [thidiazuron (N-phenyl-N1-1,2,3-thiadiazol-5-ylurea)] and 0.1 mg/l NAA was found best for P. pyrifolia Shenbuzhi, and Nitsch and Nitsch complete medium containing 3.0 mg/l TDZ and 0.1 mg/l NAA or 0.2 mg/l IAA was suitable forP. bretschneideri Zaosu or P. ussuriensis Manyuanxiang, respectively. After cutting the leaves into three sections perpendicular to the midrib and culturing under the equivalent conditions, regeneration occurred more frequently on basal sections than middle sections, and no shoots formed on apical sections. A ratio of NH+4-N/NO-3- N of 1:2~1:7 was found beneficial for shoot regeneration. 75.0-87.5% of proliferating shoots formed roots after 4 weeks of transfer to 1/4 strength of Murashige and Skoog complete medium supplemented with 2.5 mg/l IBA (indole-3-butryric acid) and 30.0 g/l sucrose. Regenerated plants were successfully established under greenhouse conditions. keywords: adventitious shoot leaf explant organogenesis pear caulogenesis pyrus Plant culture Y. Luo verfasserin aut C. Liu verfasserin aut In Plant, Soil and Environment Czech Academy of Agricultural Sciences, 2010 54(2008), 4, Seite 140-148 (DE-627)387477543 (DE-600)2145229-5 18059368 nnns volume:54 year:2008 number:4 pages:140-148 https://doi.org/10.17221/389-PSE kostenfrei https://doaj.org/article/52ecfa391d4542a9863d4021c17fdb78 kostenfrei https://pse.agriculturejournals.cz/artkey/pse-200804-0002_plant-regeneration-from-in-vitro-leaves-of-four-commercial-pyrus-species.php kostenfrei https://doaj.org/toc/1214-1178 Journal toc kostenfrei https://doaj.org/toc/1805-9368 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 54 2008 4 140-148
allfieldsGer	10.17221/389-PSE doi (DE-627)DOAJ082832439 (DE-599)DOAJ52ecfa391d4542a9863d4021c17fdb78 DE-627 ger DE-627 rakwb eng SB1-1110 H. Tang verfasserin aut Plant regeneration from in vitro leaves of four commercial Pyrus species 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier An efficient shoot regeneration from in vitro leaf sections of Pyrus communis Bartlett, P. pyrifolia Shenbuzhi, P. bretschneideri Zaosu and P. ussuriensis Manyuanxiang was successfully developed for use in future transgenic studies. On the basis of regeneration frequency and average shoot numbers, optimal shoot regeneration was obtained on leaf sections of P. communis Bartlett when cultured on Murashige and Skoog complete medium containing 6.0 mg/l BA (6-benzyladenine) and 0.1 mg/l NAA (α-naphthaleneacetic acid), while Quoirin and Lepoivre complete medium supplemented with 1.0 mg/l TDZ [thidiazuron (N-phenyl-N1-1,2,3-thiadiazol-5-ylurea)] and 0.1 mg/l NAA was found best for P. pyrifolia Shenbuzhi, and Nitsch and Nitsch complete medium containing 3.0 mg/l TDZ and 0.1 mg/l NAA or 0.2 mg/l IAA was suitable forP. bretschneideri Zaosu or P. ussuriensis Manyuanxiang, respectively. After cutting the leaves into three sections perpendicular to the midrib and culturing under the equivalent conditions, regeneration occurred more frequently on basal sections than middle sections, and no shoots formed on apical sections. A ratio of NH+4-N/NO-3- N of 1:2~1:7 was found beneficial for shoot regeneration. 75.0-87.5% of proliferating shoots formed roots after 4 weeks of transfer to 1/4 strength of Murashige and Skoog complete medium supplemented with 2.5 mg/l IBA (indole-3-butryric acid) and 30.0 g/l sucrose. Regenerated plants were successfully established under greenhouse conditions. keywords: adventitious shoot leaf explant organogenesis pear caulogenesis pyrus Plant culture Y. Luo verfasserin aut C. Liu verfasserin aut In Plant, Soil and Environment Czech Academy of Agricultural Sciences, 2010 54(2008), 4, Seite 140-148 (DE-627)387477543 (DE-600)2145229-5 18059368 nnns volume:54 year:2008 number:4 pages:140-148 https://doi.org/10.17221/389-PSE kostenfrei https://doaj.org/article/52ecfa391d4542a9863d4021c17fdb78 kostenfrei https://pse.agriculturejournals.cz/artkey/pse-200804-0002_plant-regeneration-from-in-vitro-leaves-of-four-commercial-pyrus-species.php kostenfrei https://doaj.org/toc/1214-1178 Journal toc kostenfrei https://doaj.org/toc/1805-9368 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 54 2008 4 140-148
allfieldsSound	10.17221/389-PSE doi (DE-627)DOAJ082832439 (DE-599)DOAJ52ecfa391d4542a9863d4021c17fdb78 DE-627 ger DE-627 rakwb eng SB1-1110 H. Tang verfasserin aut Plant regeneration from in vitro leaves of four commercial Pyrus species 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier An efficient shoot regeneration from in vitro leaf sections of Pyrus communis Bartlett, P. pyrifolia Shenbuzhi, P. bretschneideri Zaosu and P. ussuriensis Manyuanxiang was successfully developed for use in future transgenic studies. On the basis of regeneration frequency and average shoot numbers, optimal shoot regeneration was obtained on leaf sections of P. communis Bartlett when cultured on Murashige and Skoog complete medium containing 6.0 mg/l BA (6-benzyladenine) and 0.1 mg/l NAA (α-naphthaleneacetic acid), while Quoirin and Lepoivre complete medium supplemented with 1.0 mg/l TDZ [thidiazuron (N-phenyl-N1-1,2,3-thiadiazol-5-ylurea)] and 0.1 mg/l NAA was found best for P. pyrifolia Shenbuzhi, and Nitsch and Nitsch complete medium containing 3.0 mg/l TDZ and 0.1 mg/l NAA or 0.2 mg/l IAA was suitable forP. bretschneideri Zaosu or P. ussuriensis Manyuanxiang, respectively. After cutting the leaves into three sections perpendicular to the midrib and culturing under the equivalent conditions, regeneration occurred more frequently on basal sections than middle sections, and no shoots formed on apical sections. A ratio of NH+4-N/NO-3- N of 1:2~1:7 was found beneficial for shoot regeneration. 75.0-87.5% of proliferating shoots formed roots after 4 weeks of transfer to 1/4 strength of Murashige and Skoog complete medium supplemented with 2.5 mg/l IBA (indole-3-butryric acid) and 30.0 g/l sucrose. Regenerated plants were successfully established under greenhouse conditions. keywords: adventitious shoot leaf explant organogenesis pear caulogenesis pyrus Plant culture Y. Luo verfasserin aut C. Liu verfasserin aut In Plant, Soil and Environment Czech Academy of Agricultural Sciences, 2010 54(2008), 4, Seite 140-148 (DE-627)387477543 (DE-600)2145229-5 18059368 nnns volume:54 year:2008 number:4 pages:140-148 https://doi.org/10.17221/389-PSE kostenfrei https://doaj.org/article/52ecfa391d4542a9863d4021c17fdb78 kostenfrei https://pse.agriculturejournals.cz/artkey/pse-200804-0002_plant-regeneration-from-in-vitro-leaves-of-four-commercial-pyrus-species.php kostenfrei https://doaj.org/toc/1214-1178 Journal toc kostenfrei https://doaj.org/toc/1805-9368 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 54 2008 4 140-148
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callnumber-first	S - Agriculture
author	H. Tang
spellingShingle	H. Tang misc SB1-1110 misc keywords: adventitious shoot misc leaf explant misc organogenesis misc pear misc caulogenesis misc pyrus misc Plant culture Plant regeneration from in vitro leaves of four commercial Pyrus species
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topic_title	SB1-1110 Plant regeneration from in vitro leaves of four commercial Pyrus species keywords: adventitious shoot leaf explant organogenesis pear caulogenesis pyrus
topic	misc SB1-1110 misc keywords: adventitious shoot misc leaf explant misc organogenesis misc pear misc caulogenesis misc pyrus misc Plant culture
topic_unstemmed	misc SB1-1110 misc keywords: adventitious shoot misc leaf explant misc organogenesis misc pear misc caulogenesis misc pyrus misc Plant culture
topic_browse	misc SB1-1110 misc keywords: adventitious shoot misc leaf explant misc organogenesis misc pear misc caulogenesis misc pyrus misc Plant culture
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title	Plant regeneration from in vitro leaves of four commercial Pyrus species
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title_full	Plant regeneration from in vitro leaves of four commercial Pyrus species
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title_sort	plant regeneration from in vitro leaves of four commercial pyrus species
callnumber	SB1-1110
title_auth	Plant regeneration from in vitro leaves of four commercial Pyrus species
abstract	An efficient shoot regeneration from in vitro leaf sections of Pyrus communis Bartlett, P. pyrifolia Shenbuzhi, P. bretschneideri Zaosu and P. ussuriensis Manyuanxiang was successfully developed for use in future transgenic studies. On the basis of regeneration frequency and average shoot numbers, optimal shoot regeneration was obtained on leaf sections of P. communis Bartlett when cultured on Murashige and Skoog complete medium containing 6.0 mg/l BA (6-benzyladenine) and 0.1 mg/l NAA (α-naphthaleneacetic acid), while Quoirin and Lepoivre complete medium supplemented with 1.0 mg/l TDZ [thidiazuron (N-phenyl-N1-1,2,3-thiadiazol-5-ylurea)] and 0.1 mg/l NAA was found best for P. pyrifolia Shenbuzhi, and Nitsch and Nitsch complete medium containing 3.0 mg/l TDZ and 0.1 mg/l NAA or 0.2 mg/l IAA was suitable forP. bretschneideri Zaosu or P. ussuriensis Manyuanxiang, respectively. After cutting the leaves into three sections perpendicular to the midrib and culturing under the equivalent conditions, regeneration occurred more frequently on basal sections than middle sections, and no shoots formed on apical sections. A ratio of NH+4-N/NO-3- N of 1:2~1:7 was found beneficial for shoot regeneration. 75.0-87.5% of proliferating shoots formed roots after 4 weeks of transfer to 1/4 strength of Murashige and Skoog complete medium supplemented with 2.5 mg/l IBA (indole-3-butryric acid) and 30.0 g/l sucrose. Regenerated plants were successfully established under greenhouse conditions.
abstractGer	An efficient shoot regeneration from in vitro leaf sections of Pyrus communis Bartlett, P. pyrifolia Shenbuzhi, P. bretschneideri Zaosu and P. ussuriensis Manyuanxiang was successfully developed for use in future transgenic studies. On the basis of regeneration frequency and average shoot numbers, optimal shoot regeneration was obtained on leaf sections of P. communis Bartlett when cultured on Murashige and Skoog complete medium containing 6.0 mg/l BA (6-benzyladenine) and 0.1 mg/l NAA (α-naphthaleneacetic acid), while Quoirin and Lepoivre complete medium supplemented with 1.0 mg/l TDZ [thidiazuron (N-phenyl-N1-1,2,3-thiadiazol-5-ylurea)] and 0.1 mg/l NAA was found best for P. pyrifolia Shenbuzhi, and Nitsch and Nitsch complete medium containing 3.0 mg/l TDZ and 0.1 mg/l NAA or 0.2 mg/l IAA was suitable forP. bretschneideri Zaosu or P. ussuriensis Manyuanxiang, respectively. After cutting the leaves into three sections perpendicular to the midrib and culturing under the equivalent conditions, regeneration occurred more frequently on basal sections than middle sections, and no shoots formed on apical sections. A ratio of NH+4-N/NO-3- N of 1:2~1:7 was found beneficial for shoot regeneration. 75.0-87.5% of proliferating shoots formed roots after 4 weeks of transfer to 1/4 strength of Murashige and Skoog complete medium supplemented with 2.5 mg/l IBA (indole-3-butryric acid) and 30.0 g/l sucrose. Regenerated plants were successfully established under greenhouse conditions.
abstract_unstemmed	An efficient shoot regeneration from in vitro leaf sections of Pyrus communis Bartlett, P. pyrifolia Shenbuzhi, P. bretschneideri Zaosu and P. ussuriensis Manyuanxiang was successfully developed for use in future transgenic studies. On the basis of regeneration frequency and average shoot numbers, optimal shoot regeneration was obtained on leaf sections of P. communis Bartlett when cultured on Murashige and Skoog complete medium containing 6.0 mg/l BA (6-benzyladenine) and 0.1 mg/l NAA (α-naphthaleneacetic acid), while Quoirin and Lepoivre complete medium supplemented with 1.0 mg/l TDZ [thidiazuron (N-phenyl-N1-1,2,3-thiadiazol-5-ylurea)] and 0.1 mg/l NAA was found best for P. pyrifolia Shenbuzhi, and Nitsch and Nitsch complete medium containing 3.0 mg/l TDZ and 0.1 mg/l NAA or 0.2 mg/l IAA was suitable forP. bretschneideri Zaosu or P. ussuriensis Manyuanxiang, respectively. After cutting the leaves into three sections perpendicular to the midrib and culturing under the equivalent conditions, regeneration occurred more frequently on basal sections than middle sections, and no shoots formed on apical sections. A ratio of NH+4-N/NO-3- N of 1:2~1:7 was found beneficial for shoot regeneration. 75.0-87.5% of proliferating shoots formed roots after 4 weeks of transfer to 1/4 strength of Murashige and Skoog complete medium supplemented with 2.5 mg/l IBA (indole-3-butryric acid) and 30.0 g/l sucrose. Regenerated plants were successfully established under greenhouse conditions.
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title_short	Plant regeneration from in vitro leaves of four commercial Pyrus species
url	https://doi.org/10.17221/389-PSE https://doaj.org/article/52ecfa391d4542a9863d4021c17fdb78 https://pse.agriculturejournals.cz/artkey/pse-200804-0002_plant-regeneration-from-in-vitro-leaves-of-four-commercial-pyrus-species.php https://doaj.org/toc/1214-1178 https://doaj.org/toc/1805-9368
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up_date	2024-07-03T14:04:53.499Z
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Plant regeneration from in vitro leaves of four commercial Pyrus species

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