Analysis of a Dengue Virus Outbreak in Rosso, Senegal 2021
Senegal is hyperendemic for dengue. Since 2017, outbreaks have been noticed annually in many regions around the country, marked by the co-circulation of DENV1-3. On 8 October 2021, a Dengue virus outbreak in the Rosso health post (sentinel site of the syndromic surveillance network) located in the n...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Idrissa Dieng [verfasserIn] Mamadou Aliou Barry [verfasserIn] Cheikh Talla [verfasserIn] Bocar Sow [verfasserIn] Oumar Faye [verfasserIn] Moussa Moise Diagne [verfasserIn] Ousseynou Sene [verfasserIn] Oumar Ndiaye [verfasserIn] Boly Diop [verfasserIn] Cheikh Tidiane Diagne [verfasserIn] Gamou Fall [verfasserIn] Amadou Alpha Sall [verfasserIn] Cheikh Loucoubar [verfasserIn] Ousmane Faye [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2022 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Tropical Medicine and Infectious Disease - MDPI AG, 2017, 7(2022), 12, p 420 |
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| Übergeordnetes Werk: |
volume:7 ; year:2022 ; number:12, p 420 |
| Links: |
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| DOI / URN: |
10.3390/tropicalmed7120420 |
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| Katalog-ID: |
DOAJ082960852 |
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| 520 | |a Senegal is hyperendemic for dengue. Since 2017, outbreaks have been noticed annually in many regions around the country, marked by the co-circulation of DENV1-3. On 8 October 2021, a Dengue virus outbreak in the Rosso health post (sentinel site of the syndromic surveillance network) located in the north of the country was notified to the WHO Collaborating Center for arboviruses and hemorrhagic fever viruses at Institut Pasteur de Dakar. A multidisciplinary team was then sent for epidemiological and virologic investigations. This study describes the results from investigations during an outbreak in Senegal using a rapid diagnostic test (RDT) for the combined detection of dengue virus non-structural protein 1 (NS1) and IgM/IgG. For confirmation, samples were also tested by real-time RT-PCR and IgM ELISA at the reference lab in Dakar. qRT-PCR positive samples were subjected to whole genome sequencing using nanopore technology. Virologic analysis scored 102 positives cases (RT-PCR, NS1 antigen detection and/or IgM) out of 173 enrolled patients; interestingly, virus serotyping showed that the outbreak was caused by the DENV-1, a serotype different from DENV-2 involved during the outbreak in Rosso three years earlier, indicating a serotype replacement. Nearly all field-tested NS1 positives samples were confirmed by qRT-PCR with a concordance of 92.3%. Whole genome sequencing and phylogenetic analysis of strains suggested a re-introduction in Rosso of a DENV-1 strain different to the one responsible for the outbreak in the Louga area five years before. Findings call for improved dengue virus surveillance in Senegal, with a wide deployment of DENV antigenic tests, which allow easy on-site diagnosis of suspected cases and early detection of outbreaks. This work highlights the need for continuous monitoring of circulating serotypes which is crucial for a better understanding of viral epidemiology around the country. | ||
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10.3390/tropicalmed7120420 doi (DE-627)DOAJ082960852 (DE-599)DOAJc18fd9afd92d456492fc6526c4573a18 DE-627 ger DE-627 rakwb eng Idrissa Dieng verfasserin aut Analysis of a Dengue Virus Outbreak in Rosso, Senegal 2021 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Senegal is hyperendemic for dengue. Since 2017, outbreaks have been noticed annually in many regions around the country, marked by the co-circulation of DENV1-3. On 8 October 2021, a Dengue virus outbreak in the Rosso health post (sentinel site of the syndromic surveillance network) located in the north of the country was notified to the WHO Collaborating Center for arboviruses and hemorrhagic fever viruses at Institut Pasteur de Dakar. A multidisciplinary team was then sent for epidemiological and virologic investigations. This study describes the results from investigations during an outbreak in Senegal using a rapid diagnostic test (RDT) for the combined detection of dengue virus non-structural protein 1 (NS1) and IgM/IgG. For confirmation, samples were also tested by real-time RT-PCR and IgM ELISA at the reference lab in Dakar. qRT-PCR positive samples were subjected to whole genome sequencing using nanopore technology. Virologic analysis scored 102 positives cases (RT-PCR, NS1 antigen detection and/or IgM) out of 173 enrolled patients; interestingly, virus serotyping showed that the outbreak was caused by the DENV-1, a serotype different from DENV-2 involved during the outbreak in Rosso three years earlier, indicating a serotype replacement. Nearly all field-tested NS1 positives samples were confirmed by qRT-PCR with a concordance of 92.3%. Whole genome sequencing and phylogenetic analysis of strains suggested a re-introduction in Rosso of a DENV-1 strain different to the one responsible for the outbreak in the Louga area five years before. Findings call for improved dengue virus surveillance in Senegal, with a wide deployment of DENV antigenic tests, which allow easy on-site diagnosis of suspected cases and early detection of outbreaks. This work highlights the need for continuous monitoring of circulating serotypes which is crucial for a better understanding of viral epidemiology around the country. DENV-1 Rosso NS1 RDTs outbreak response serotype replacement re-introduction Medicine R Mamadou Aliou Barry verfasserin aut Cheikh Talla verfasserin aut Bocar Sow verfasserin aut Oumar Faye verfasserin aut Moussa Moise Diagne verfasserin aut Ousseynou Sene verfasserin aut Oumar Ndiaye verfasserin aut Boly Diop verfasserin aut Cheikh Tidiane Diagne verfasserin aut Gamou Fall verfasserin aut Amadou Alpha Sall verfasserin aut Cheikh Loucoubar verfasserin aut Ousmane Faye verfasserin aut In Tropical Medicine and Infectious Disease MDPI AG, 2017 7(2022), 12, p 420 (DE-627)102556488X 24146366 nnns volume:7 year:2022 number:12, p 420 https://doi.org/10.3390/tropicalmed7120420 kostenfrei https://doaj.org/article/c18fd9afd92d456492fc6526c4573a18 kostenfrei https://www.mdpi.com/2414-6366/7/12/420 kostenfrei https://doaj.org/toc/2414-6366 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2022 12, p 420 |
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10.3390/tropicalmed7120420 doi (DE-627)DOAJ082960852 (DE-599)DOAJc18fd9afd92d456492fc6526c4573a18 DE-627 ger DE-627 rakwb eng Idrissa Dieng verfasserin aut Analysis of a Dengue Virus Outbreak in Rosso, Senegal 2021 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Senegal is hyperendemic for dengue. Since 2017, outbreaks have been noticed annually in many regions around the country, marked by the co-circulation of DENV1-3. On 8 October 2021, a Dengue virus outbreak in the Rosso health post (sentinel site of the syndromic surveillance network) located in the north of the country was notified to the WHO Collaborating Center for arboviruses and hemorrhagic fever viruses at Institut Pasteur de Dakar. A multidisciplinary team was then sent for epidemiological and virologic investigations. This study describes the results from investigations during an outbreak in Senegal using a rapid diagnostic test (RDT) for the combined detection of dengue virus non-structural protein 1 (NS1) and IgM/IgG. For confirmation, samples were also tested by real-time RT-PCR and IgM ELISA at the reference lab in Dakar. qRT-PCR positive samples were subjected to whole genome sequencing using nanopore technology. Virologic analysis scored 102 positives cases (RT-PCR, NS1 antigen detection and/or IgM) out of 173 enrolled patients; interestingly, virus serotyping showed that the outbreak was caused by the DENV-1, a serotype different from DENV-2 involved during the outbreak in Rosso three years earlier, indicating a serotype replacement. Nearly all field-tested NS1 positives samples were confirmed by qRT-PCR with a concordance of 92.3%. Whole genome sequencing and phylogenetic analysis of strains suggested a re-introduction in Rosso of a DENV-1 strain different to the one responsible for the outbreak in the Louga area five years before. Findings call for improved dengue virus surveillance in Senegal, with a wide deployment of DENV antigenic tests, which allow easy on-site diagnosis of suspected cases and early detection of outbreaks. This work highlights the need for continuous monitoring of circulating serotypes which is crucial for a better understanding of viral epidemiology around the country. DENV-1 Rosso NS1 RDTs outbreak response serotype replacement re-introduction Medicine R Mamadou Aliou Barry verfasserin aut Cheikh Talla verfasserin aut Bocar Sow verfasserin aut Oumar Faye verfasserin aut Moussa Moise Diagne verfasserin aut Ousseynou Sene verfasserin aut Oumar Ndiaye verfasserin aut Boly Diop verfasserin aut Cheikh Tidiane Diagne verfasserin aut Gamou Fall verfasserin aut Amadou Alpha Sall verfasserin aut Cheikh Loucoubar verfasserin aut Ousmane Faye verfasserin aut In Tropical Medicine and Infectious Disease MDPI AG, 2017 7(2022), 12, p 420 (DE-627)102556488X 24146366 nnns volume:7 year:2022 number:12, p 420 https://doi.org/10.3390/tropicalmed7120420 kostenfrei https://doaj.org/article/c18fd9afd92d456492fc6526c4573a18 kostenfrei https://www.mdpi.com/2414-6366/7/12/420 kostenfrei https://doaj.org/toc/2414-6366 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2022 12, p 420 |
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10.3390/tropicalmed7120420 doi (DE-627)DOAJ082960852 (DE-599)DOAJc18fd9afd92d456492fc6526c4573a18 DE-627 ger DE-627 rakwb eng Idrissa Dieng verfasserin aut Analysis of a Dengue Virus Outbreak in Rosso, Senegal 2021 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Senegal is hyperendemic for dengue. Since 2017, outbreaks have been noticed annually in many regions around the country, marked by the co-circulation of DENV1-3. On 8 October 2021, a Dengue virus outbreak in the Rosso health post (sentinel site of the syndromic surveillance network) located in the north of the country was notified to the WHO Collaborating Center for arboviruses and hemorrhagic fever viruses at Institut Pasteur de Dakar. A multidisciplinary team was then sent for epidemiological and virologic investigations. This study describes the results from investigations during an outbreak in Senegal using a rapid diagnostic test (RDT) for the combined detection of dengue virus non-structural protein 1 (NS1) and IgM/IgG. For confirmation, samples were also tested by real-time RT-PCR and IgM ELISA at the reference lab in Dakar. qRT-PCR positive samples were subjected to whole genome sequencing using nanopore technology. Virologic analysis scored 102 positives cases (RT-PCR, NS1 antigen detection and/or IgM) out of 173 enrolled patients; interestingly, virus serotyping showed that the outbreak was caused by the DENV-1, a serotype different from DENV-2 involved during the outbreak in Rosso three years earlier, indicating a serotype replacement. Nearly all field-tested NS1 positives samples were confirmed by qRT-PCR with a concordance of 92.3%. Whole genome sequencing and phylogenetic analysis of strains suggested a re-introduction in Rosso of a DENV-1 strain different to the one responsible for the outbreak in the Louga area five years before. Findings call for improved dengue virus surveillance in Senegal, with a wide deployment of DENV antigenic tests, which allow easy on-site diagnosis of suspected cases and early detection of outbreaks. This work highlights the need for continuous monitoring of circulating serotypes which is crucial for a better understanding of viral epidemiology around the country. DENV-1 Rosso NS1 RDTs outbreak response serotype replacement re-introduction Medicine R Mamadou Aliou Barry verfasserin aut Cheikh Talla verfasserin aut Bocar Sow verfasserin aut Oumar Faye verfasserin aut Moussa Moise Diagne verfasserin aut Ousseynou Sene verfasserin aut Oumar Ndiaye verfasserin aut Boly Diop verfasserin aut Cheikh Tidiane Diagne verfasserin aut Gamou Fall verfasserin aut Amadou Alpha Sall verfasserin aut Cheikh Loucoubar verfasserin aut Ousmane Faye verfasserin aut In Tropical Medicine and Infectious Disease MDPI AG, 2017 7(2022), 12, p 420 (DE-627)102556488X 24146366 nnns volume:7 year:2022 number:12, p 420 https://doi.org/10.3390/tropicalmed7120420 kostenfrei https://doaj.org/article/c18fd9afd92d456492fc6526c4573a18 kostenfrei https://www.mdpi.com/2414-6366/7/12/420 kostenfrei https://doaj.org/toc/2414-6366 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2022 12, p 420 |
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10.3390/tropicalmed7120420 doi (DE-627)DOAJ082960852 (DE-599)DOAJc18fd9afd92d456492fc6526c4573a18 DE-627 ger DE-627 rakwb eng Idrissa Dieng verfasserin aut Analysis of a Dengue Virus Outbreak in Rosso, Senegal 2021 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Senegal is hyperendemic for dengue. Since 2017, outbreaks have been noticed annually in many regions around the country, marked by the co-circulation of DENV1-3. On 8 October 2021, a Dengue virus outbreak in the Rosso health post (sentinel site of the syndromic surveillance network) located in the north of the country was notified to the WHO Collaborating Center for arboviruses and hemorrhagic fever viruses at Institut Pasteur de Dakar. A multidisciplinary team was then sent for epidemiological and virologic investigations. This study describes the results from investigations during an outbreak in Senegal using a rapid diagnostic test (RDT) for the combined detection of dengue virus non-structural protein 1 (NS1) and IgM/IgG. For confirmation, samples were also tested by real-time RT-PCR and IgM ELISA at the reference lab in Dakar. qRT-PCR positive samples were subjected to whole genome sequencing using nanopore technology. Virologic analysis scored 102 positives cases (RT-PCR, NS1 antigen detection and/or IgM) out of 173 enrolled patients; interestingly, virus serotyping showed that the outbreak was caused by the DENV-1, a serotype different from DENV-2 involved during the outbreak in Rosso three years earlier, indicating a serotype replacement. Nearly all field-tested NS1 positives samples were confirmed by qRT-PCR with a concordance of 92.3%. Whole genome sequencing and phylogenetic analysis of strains suggested a re-introduction in Rosso of a DENV-1 strain different to the one responsible for the outbreak in the Louga area five years before. Findings call for improved dengue virus surveillance in Senegal, with a wide deployment of DENV antigenic tests, which allow easy on-site diagnosis of suspected cases and early detection of outbreaks. This work highlights the need for continuous monitoring of circulating serotypes which is crucial for a better understanding of viral epidemiology around the country. DENV-1 Rosso NS1 RDTs outbreak response serotype replacement re-introduction Medicine R Mamadou Aliou Barry verfasserin aut Cheikh Talla verfasserin aut Bocar Sow verfasserin aut Oumar Faye verfasserin aut Moussa Moise Diagne verfasserin aut Ousseynou Sene verfasserin aut Oumar Ndiaye verfasserin aut Boly Diop verfasserin aut Cheikh Tidiane Diagne verfasserin aut Gamou Fall verfasserin aut Amadou Alpha Sall verfasserin aut Cheikh Loucoubar verfasserin aut Ousmane Faye verfasserin aut In Tropical Medicine and Infectious Disease MDPI AG, 2017 7(2022), 12, p 420 (DE-627)102556488X 24146366 nnns volume:7 year:2022 number:12, p 420 https://doi.org/10.3390/tropicalmed7120420 kostenfrei https://doaj.org/article/c18fd9afd92d456492fc6526c4573a18 kostenfrei https://www.mdpi.com/2414-6366/7/12/420 kostenfrei https://doaj.org/toc/2414-6366 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2022 12, p 420 |
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Analysis of a Dengue Virus Outbreak in Rosso, Senegal 2021 |
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Senegal is hyperendemic for dengue. Since 2017, outbreaks have been noticed annually in many regions around the country, marked by the co-circulation of DENV1-3. On 8 October 2021, a Dengue virus outbreak in the Rosso health post (sentinel site of the syndromic surveillance network) located in the north of the country was notified to the WHO Collaborating Center for arboviruses and hemorrhagic fever viruses at Institut Pasteur de Dakar. A multidisciplinary team was then sent for epidemiological and virologic investigations. This study describes the results from investigations during an outbreak in Senegal using a rapid diagnostic test (RDT) for the combined detection of dengue virus non-structural protein 1 (NS1) and IgM/IgG. For confirmation, samples were also tested by real-time RT-PCR and IgM ELISA at the reference lab in Dakar. qRT-PCR positive samples were subjected to whole genome sequencing using nanopore technology. Virologic analysis scored 102 positives cases (RT-PCR, NS1 antigen detection and/or IgM) out of 173 enrolled patients; interestingly, virus serotyping showed that the outbreak was caused by the DENV-1, a serotype different from DENV-2 involved during the outbreak in Rosso three years earlier, indicating a serotype replacement. Nearly all field-tested NS1 positives samples were confirmed by qRT-PCR with a concordance of 92.3%. Whole genome sequencing and phylogenetic analysis of strains suggested a re-introduction in Rosso of a DENV-1 strain different to the one responsible for the outbreak in the Louga area five years before. Findings call for improved dengue virus surveillance in Senegal, with a wide deployment of DENV antigenic tests, which allow easy on-site diagnosis of suspected cases and early detection of outbreaks. This work highlights the need for continuous monitoring of circulating serotypes which is crucial for a better understanding of viral epidemiology around the country. |
| abstractGer |
Senegal is hyperendemic for dengue. Since 2017, outbreaks have been noticed annually in many regions around the country, marked by the co-circulation of DENV1-3. On 8 October 2021, a Dengue virus outbreak in the Rosso health post (sentinel site of the syndromic surveillance network) located in the north of the country was notified to the WHO Collaborating Center for arboviruses and hemorrhagic fever viruses at Institut Pasteur de Dakar. A multidisciplinary team was then sent for epidemiological and virologic investigations. This study describes the results from investigations during an outbreak in Senegal using a rapid diagnostic test (RDT) for the combined detection of dengue virus non-structural protein 1 (NS1) and IgM/IgG. For confirmation, samples were also tested by real-time RT-PCR and IgM ELISA at the reference lab in Dakar. qRT-PCR positive samples were subjected to whole genome sequencing using nanopore technology. Virologic analysis scored 102 positives cases (RT-PCR, NS1 antigen detection and/or IgM) out of 173 enrolled patients; interestingly, virus serotyping showed that the outbreak was caused by the DENV-1, a serotype different from DENV-2 involved during the outbreak in Rosso three years earlier, indicating a serotype replacement. Nearly all field-tested NS1 positives samples were confirmed by qRT-PCR with a concordance of 92.3%. Whole genome sequencing and phylogenetic analysis of strains suggested a re-introduction in Rosso of a DENV-1 strain different to the one responsible for the outbreak in the Louga area five years before. Findings call for improved dengue virus surveillance in Senegal, with a wide deployment of DENV antigenic tests, which allow easy on-site diagnosis of suspected cases and early detection of outbreaks. This work highlights the need for continuous monitoring of circulating serotypes which is crucial for a better understanding of viral epidemiology around the country. |
| abstract_unstemmed |
Senegal is hyperendemic for dengue. Since 2017, outbreaks have been noticed annually in many regions around the country, marked by the co-circulation of DENV1-3. On 8 October 2021, a Dengue virus outbreak in the Rosso health post (sentinel site of the syndromic surveillance network) located in the north of the country was notified to the WHO Collaborating Center for arboviruses and hemorrhagic fever viruses at Institut Pasteur de Dakar. A multidisciplinary team was then sent for epidemiological and virologic investigations. This study describes the results from investigations during an outbreak in Senegal using a rapid diagnostic test (RDT) for the combined detection of dengue virus non-structural protein 1 (NS1) and IgM/IgG. For confirmation, samples were also tested by real-time RT-PCR and IgM ELISA at the reference lab in Dakar. qRT-PCR positive samples were subjected to whole genome sequencing using nanopore technology. Virologic analysis scored 102 positives cases (RT-PCR, NS1 antigen detection and/or IgM) out of 173 enrolled patients; interestingly, virus serotyping showed that the outbreak was caused by the DENV-1, a serotype different from DENV-2 involved during the outbreak in Rosso three years earlier, indicating a serotype replacement. Nearly all field-tested NS1 positives samples were confirmed by qRT-PCR with a concordance of 92.3%. Whole genome sequencing and phylogenetic analysis of strains suggested a re-introduction in Rosso of a DENV-1 strain different to the one responsible for the outbreak in the Louga area five years before. Findings call for improved dengue virus surveillance in Senegal, with a wide deployment of DENV antigenic tests, which allow easy on-site diagnosis of suspected cases and early detection of outbreaks. This work highlights the need for continuous monitoring of circulating serotypes which is crucial for a better understanding of viral epidemiology around the country. |
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| container_issue |
12, p 420 |
| title_short |
Analysis of a Dengue Virus Outbreak in Rosso, Senegal 2021 |
| url |
https://doi.org/10.3390/tropicalmed7120420 https://doaj.org/article/c18fd9afd92d456492fc6526c4573a18 https://www.mdpi.com/2414-6366/7/12/420 https://doaj.org/toc/2414-6366 |
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| author2 |
Mamadou Aliou Barry Cheikh Talla Bocar Sow Oumar Faye Moussa Moise Diagne Ousseynou Sene Oumar Ndiaye Boly Diop Cheikh Tidiane Diagne Gamou Fall Amadou Alpha Sall Cheikh Loucoubar Ousmane Faye |
| author2Str |
Mamadou Aliou Barry Cheikh Talla Bocar Sow Oumar Faye Moussa Moise Diagne Ousseynou Sene Oumar Ndiaye Boly Diop Cheikh Tidiane Diagne Gamou Fall Amadou Alpha Sall Cheikh Loucoubar Ousmane Faye |
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| doi_str |
10.3390/tropicalmed7120420 |
| up_date |
2024-07-03T14:46:05.086Z |
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