Virtual Filter Membranes in a Microfluidic System for Sorting and Separating Size-Based Micro Polystyrene Beads by Illumination Intensity Design in Optically Induced Dielectrophoresis (ODEP)

In biomedical diagnosis, the efficient separation and purification of specific targets from clinical samples is the desired first step. Herein, the concept of virtual filter membranes based on optically-induced dielectrophoresis (ODEP) manipulation in a microfluidic channel is proposed as a light sc...
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Autor*in:	Chia-Ming Yang [verfasserIn] Ai-Yun Wu [verfasserIn] Jian-Cyun Yu [verfasserIn] Po-Yu Chu [verfasserIn] Chia-Hsun Hsieh [verfasserIn] Min-Hsien Wu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	brightness light screening membrane microfluidic ODEP polystyrene

Übergeordnetes Werk:	In: Chemosensors - MDPI AG, 2013, 10(2022), 12, p 540
Übergeordnetes Werk:	volume:10 ; year:2022 ; number:12, p 540

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/chemosensors10120540

Katalog-ID:	DOAJ083207554

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language	English
source	In Chemosensors 10(2022), 12, p 540 volume:10 year:2022 number:12, p 540
sourceStr	In Chemosensors 10(2022), 12, p 540 volume:10 year:2022 number:12, p 540
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	brightness light screening membrane microfluidic ODEP polystyrene Biochemistry
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container_title	Chemosensors
authorswithroles_txt_mv	Chia-Ming Yang @@aut@@ Ai-Yun Wu @@aut@@ Jian-Cyun Yu @@aut@@ Po-Yu Chu @@aut@@ Chia-Hsun Hsieh @@aut@@ Min-Hsien Wu @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
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callnumber-first	Q - Science
author	Chia-Ming Yang
spellingShingle	Chia-Ming Yang misc QD415-436 misc brightness misc light screening membrane misc microfluidic misc ODEP misc polystyrene misc Biochemistry Virtual Filter Membranes in a Microfluidic System for Sorting and Separating Size-Based Micro Polystyrene Beads by Illumination Intensity Design in Optically Induced Dielectrophoresis (ODEP)
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topic_title	QD415-436 Virtual Filter Membranes in a Microfluidic System for Sorting and Separating Size-Based Micro Polystyrene Beads by Illumination Intensity Design in Optically Induced Dielectrophoresis (ODEP) brightness light screening membrane microfluidic ODEP polystyrene
topic	misc QD415-436 misc brightness misc light screening membrane misc microfluidic misc ODEP misc polystyrene misc Biochemistry
topic_unstemmed	misc QD415-436 misc brightness misc light screening membrane misc microfluidic misc ODEP misc polystyrene misc Biochemistry
topic_browse	misc QD415-436 misc brightness misc light screening membrane misc microfluidic misc ODEP misc polystyrene misc Biochemistry
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title	Virtual Filter Membranes in a Microfluidic System for Sorting and Separating Size-Based Micro Polystyrene Beads by Illumination Intensity Design in Optically Induced Dielectrophoresis (ODEP)
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title_full	Virtual Filter Membranes in a Microfluidic System for Sorting and Separating Size-Based Micro Polystyrene Beads by Illumination Intensity Design in Optically Induced Dielectrophoresis (ODEP)
author_sort	Chia-Ming Yang
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author_browse	Chia-Ming Yang Ai-Yun Wu Jian-Cyun Yu Po-Yu Chu Chia-Hsun Hsieh Min-Hsien Wu
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title_sort	virtual filter membranes in a microfluidic system for sorting and separating size-based micro polystyrene beads by illumination intensity design in optically induced dielectrophoresis (odep)
callnumber	QD415-436
title_auth	Virtual Filter Membranes in a Microfluidic System for Sorting and Separating Size-Based Micro Polystyrene Beads by Illumination Intensity Design in Optically Induced Dielectrophoresis (ODEP)
abstract	In biomedical diagnosis, the efficient separation and purification of specific targets from clinical samples is the desired first step. Herein, the concept of virtual filter membranes based on optically-induced dielectrophoresis (ODEP) manipulation in a microfluidic channel is proposed as a light screening membrane for the separation of polystyrene (PS) microparticles with three different diameters of 15.8, 10.8 and 5.8 µm. The ODEP manipulation velocity of three types of PS microparticles reacted with the color brightness setting was investigated to determine the light intensity to induce an ODEP force higher than the drag force of fluid speed. The color brightness of the light bar in three areas of the light screening membrane was selected as 60%, 70% and 100% to isolate PS microparticles with diameters of 15.8, 10.8 and 5.8 µm, respectively. With a double light bar and a flow rate of 3 µL/min, the recovery rate and isolation purity was improved by 95.1~100% and 94.4~98.6% from the mixture of three types of PS microparticles within 2 min, respectively. This proposed light screening membrane could be a candidate for the separation of small-volume and rare biomedical samples, including circulating tumor cells (CTCs) and bacteria in the blood.
abstractGer	In biomedical diagnosis, the efficient separation and purification of specific targets from clinical samples is the desired first step. Herein, the concept of virtual filter membranes based on optically-induced dielectrophoresis (ODEP) manipulation in a microfluidic channel is proposed as a light screening membrane for the separation of polystyrene (PS) microparticles with three different diameters of 15.8, 10.8 and 5.8 µm. The ODEP manipulation velocity of three types of PS microparticles reacted with the color brightness setting was investigated to determine the light intensity to induce an ODEP force higher than the drag force of fluid speed. The color brightness of the light bar in three areas of the light screening membrane was selected as 60%, 70% and 100% to isolate PS microparticles with diameters of 15.8, 10.8 and 5.8 µm, respectively. With a double light bar and a flow rate of 3 µL/min, the recovery rate and isolation purity was improved by 95.1~100% and 94.4~98.6% from the mixture of three types of PS microparticles within 2 min, respectively. This proposed light screening membrane could be a candidate for the separation of small-volume and rare biomedical samples, including circulating tumor cells (CTCs) and bacteria in the blood.
abstract_unstemmed	In biomedical diagnosis, the efficient separation and purification of specific targets from clinical samples is the desired first step. Herein, the concept of virtual filter membranes based on optically-induced dielectrophoresis (ODEP) manipulation in a microfluidic channel is proposed as a light screening membrane for the separation of polystyrene (PS) microparticles with three different diameters of 15.8, 10.8 and 5.8 µm. The ODEP manipulation velocity of three types of PS microparticles reacted with the color brightness setting was investigated to determine the light intensity to induce an ODEP force higher than the drag force of fluid speed. The color brightness of the light bar in three areas of the light screening membrane was selected as 60%, 70% and 100% to isolate PS microparticles with diameters of 15.8, 10.8 and 5.8 µm, respectively. With a double light bar and a flow rate of 3 µL/min, the recovery rate and isolation purity was improved by 95.1~100% and 94.4~98.6% from the mixture of three types of PS microparticles within 2 min, respectively. This proposed light screening membrane could be a candidate for the separation of small-volume and rare biomedical samples, including circulating tumor cells (CTCs) and bacteria in the blood.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
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title_short	Virtual Filter Membranes in a Microfluidic System for Sorting and Separating Size-Based Micro Polystyrene Beads by Illumination Intensity Design in Optically Induced Dielectrophoresis (ODEP)
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author2	Ai-Yun Wu Jian-Cyun Yu Po-Yu Chu Chia-Hsun Hsieh Min-Hsien Wu
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up_date	2024-07-03T16:09:34.076Z
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Virtual Filter Membranes in a Microfluidic System for Sorting and Separating Size-Based Micro Polystyrene Beads by Illumination Intensity Design in Optically Induced Dielectrophoresis (ODEP)

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