<i<LmCht5-1</i< and <i<LmCht5-2</i< Promote the Degradation of Serosal and Pro-Nymphal Cuticles during Locust Embryonic Development

The success of the degradation of the extraembryonic serosal cuticle and the second embryonic cuticle (pro-nymphal cuticle) is essential for the development and molting of nymph from egg in Orthoptera <i<Locusta migratoria</i<. Chitinase 5 is an important gene for chitin degradation in n...
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Autor*in:	Tingting Zhang [verfasserIn] Yanjun Huo [verfasserIn] Qing Dong [verfasserIn] Weiwei Liu [verfasserIn] Lu Gao [verfasserIn] Jiannan Zhou [verfasserIn] Daqi Li [verfasserIn] Xueyao Zhang [verfasserIn] Jianzhen Zhang [verfasserIn] Min Zhang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	serosal cuticle pro-nymphal cuticle Chitinase 5-1 Chitinase 5-2

Übergeordnetes Werk:	In: Biology - MDPI AG, 2012, 11(2022), 12, p 1778
Übergeordnetes Werk:	volume:11 ; year:2022 ; number:12, p 1778

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/biology11121778

Katalog-ID:	DOAJ083228829

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520			\|a The success of the degradation of the extraembryonic serosal cuticle and the second embryonic cuticle (pro-nymphal cuticle) is essential for the development and molting of nymph from egg in Orthoptera <i<Locusta migratoria</i<. Chitinase 5 is an important gene for chitin degradation in nymphs and in the egg stage. In this study, we investigated the important roles of chitinase 5-1 (<i<LmCht5-1</i<) and chitinase 5-2 (<i<LmCht5-2</i<) in the degradation of the serosal and pro-nymphal cuticles during locust embryonic development. The serosal cuticle degrades from 7-day-old embryos (E7) to E13, along with the degradation of the pro-nymphal cuticle, which begins at E12 to E14. The mRNA and protein of <i<LmCht5-1</i< and <i<LmCht5-2</i< are expressed during the degradation process of the serosal cuticle and the pro-nymphal cuticle. RNAi experiments at the embryonic stage show that both ds<i<LmCht5-1</i< and ds<i<LmCht5-2</i< contribute to the failure of development in early and late embryogenesis. Further, during the serosal cuticle molting process, ultra-structure analysis indicated that ds<i<LmCht5-1</i< prevented the loss of the coarse chitin layer in the upper part in both early and late embryogenesis. Meanwhile, ds<i<LmCht5-2</i< blocked the degradation of the lower fine chitin layer at the early stage and blocked the chitin degradation of loose coarse chitin in the late molting process. During the degradation of the pro-nymphal cuticle, ds<i<LmCht5-1</i< suppresses chitin degradation between layers in the procuticle, while ds<i<LmCht5-2</i< suppresses chitin degradation into filaments inside of the layer. In summary, our results suggest that both <i<LmCht5-1</i< and <i<LmCht5-2</i< contribute to the degradation of the serosal and pro-nymphal cuticles during the locust embryonic stage.
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allfields	10.3390/biology11121778 doi (DE-627)DOAJ083228829 (DE-599)DOAJ50f35e3834914158988062cfb21745a0 DE-627 ger DE-627 rakwb eng QH301-705.5 Tingting Zhang verfasserin aut <i<LmCht5-1</i< and <i<LmCht5-2</i< Promote the Degradation of Serosal and Pro-Nymphal Cuticles during Locust Embryonic Development 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The success of the degradation of the extraembryonic serosal cuticle and the second embryonic cuticle (pro-nymphal cuticle) is essential for the development and molting of nymph from egg in Orthoptera <i<Locusta migratoria</i<. Chitinase 5 is an important gene for chitin degradation in nymphs and in the egg stage. In this study, we investigated the important roles of chitinase 5-1 (<i<LmCht5-1</i<) and chitinase 5-2 (<i<LmCht5-2</i<) in the degradation of the serosal and pro-nymphal cuticles during locust embryonic development. The serosal cuticle degrades from 7-day-old embryos (E7) to E13, along with the degradation of the pro-nymphal cuticle, which begins at E12 to E14. The mRNA and protein of <i<LmCht5-1</i< and <i<LmCht5-2</i< are expressed during the degradation process of the serosal cuticle and the pro-nymphal cuticle. RNAi experiments at the embryonic stage show that both ds<i<LmCht5-1</i< and ds<i<LmCht5-2</i< contribute to the failure of development in early and late embryogenesis. Further, during the serosal cuticle molting process, ultra-structure analysis indicated that ds<i<LmCht5-1</i< prevented the loss of the coarse chitin layer in the upper part in both early and late embryogenesis. Meanwhile, ds<i<LmCht5-2</i< blocked the degradation of the lower fine chitin layer at the early stage and blocked the chitin degradation of loose coarse chitin in the late molting process. During the degradation of the pro-nymphal cuticle, ds<i<LmCht5-1</i< suppresses chitin degradation between layers in the procuticle, while ds<i<LmCht5-2</i< suppresses chitin degradation into filaments inside of the layer. In summary, our results suggest that both <i<LmCht5-1</i< and <i<LmCht5-2</i< contribute to the degradation of the serosal and pro-nymphal cuticles during the locust embryonic stage. <i<Locust migratoria</i< serosal cuticle pro-nymphal cuticle Chitinase 5-1 Chitinase 5-2 Biology (General) Yanjun Huo verfasserin aut Qing Dong verfasserin aut Weiwei Liu verfasserin aut Lu Gao verfasserin aut Jiannan Zhou verfasserin aut Daqi Li verfasserin aut Xueyao Zhang verfasserin aut Jianzhen Zhang verfasserin aut Min Zhang verfasserin aut In Biology MDPI AG, 2012 11(2022), 12, p 1778 (DE-627)718622073 (DE-600)2661517-4 20797737 nnns volume:11 year:2022 number:12, p 1778 https://doi.org/10.3390/biology11121778 kostenfrei https://doaj.org/article/50f35e3834914158988062cfb21745a0 kostenfrei https://www.mdpi.com/2079-7737/11/12/1778 kostenfrei https://doaj.org/toc/2079-7737 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 12, p 1778
spelling	10.3390/biology11121778 doi (DE-627)DOAJ083228829 (DE-599)DOAJ50f35e3834914158988062cfb21745a0 DE-627 ger DE-627 rakwb eng QH301-705.5 Tingting Zhang verfasserin aut <i<LmCht5-1</i< and <i<LmCht5-2</i< Promote the Degradation of Serosal and Pro-Nymphal Cuticles during Locust Embryonic Development 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The success of the degradation of the extraembryonic serosal cuticle and the second embryonic cuticle (pro-nymphal cuticle) is essential for the development and molting of nymph from egg in Orthoptera <i<Locusta migratoria</i<. Chitinase 5 is an important gene for chitin degradation in nymphs and in the egg stage. In this study, we investigated the important roles of chitinase 5-1 (<i<LmCht5-1</i<) and chitinase 5-2 (<i<LmCht5-2</i<) in the degradation of the serosal and pro-nymphal cuticles during locust embryonic development. The serosal cuticle degrades from 7-day-old embryos (E7) to E13, along with the degradation of the pro-nymphal cuticle, which begins at E12 to E14. The mRNA and protein of <i<LmCht5-1</i< and <i<LmCht5-2</i< are expressed during the degradation process of the serosal cuticle and the pro-nymphal cuticle. RNAi experiments at the embryonic stage show that both ds<i<LmCht5-1</i< and ds<i<LmCht5-2</i< contribute to the failure of development in early and late embryogenesis. Further, during the serosal cuticle molting process, ultra-structure analysis indicated that ds<i<LmCht5-1</i< prevented the loss of the coarse chitin layer in the upper part in both early and late embryogenesis. Meanwhile, ds<i<LmCht5-2</i< blocked the degradation of the lower fine chitin layer at the early stage and blocked the chitin degradation of loose coarse chitin in the late molting process. During the degradation of the pro-nymphal cuticle, ds<i<LmCht5-1</i< suppresses chitin degradation between layers in the procuticle, while ds<i<LmCht5-2</i< suppresses chitin degradation into filaments inside of the layer. In summary, our results suggest that both <i<LmCht5-1</i< and <i<LmCht5-2</i< contribute to the degradation of the serosal and pro-nymphal cuticles during the locust embryonic stage. <i<Locust migratoria</i< serosal cuticle pro-nymphal cuticle Chitinase 5-1 Chitinase 5-2 Biology (General) Yanjun Huo verfasserin aut Qing Dong verfasserin aut Weiwei Liu verfasserin aut Lu Gao verfasserin aut Jiannan Zhou verfasserin aut Daqi Li verfasserin aut Xueyao Zhang verfasserin aut Jianzhen Zhang verfasserin aut Min Zhang verfasserin aut In Biology MDPI AG, 2012 11(2022), 12, p 1778 (DE-627)718622073 (DE-600)2661517-4 20797737 nnns volume:11 year:2022 number:12, p 1778 https://doi.org/10.3390/biology11121778 kostenfrei https://doaj.org/article/50f35e3834914158988062cfb21745a0 kostenfrei https://www.mdpi.com/2079-7737/11/12/1778 kostenfrei https://doaj.org/toc/2079-7737 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 12, p 1778
allfields_unstemmed	10.3390/biology11121778 doi (DE-627)DOAJ083228829 (DE-599)DOAJ50f35e3834914158988062cfb21745a0 DE-627 ger DE-627 rakwb eng QH301-705.5 Tingting Zhang verfasserin aut <i<LmCht5-1</i< and <i<LmCht5-2</i< Promote the Degradation of Serosal and Pro-Nymphal Cuticles during Locust Embryonic Development 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The success of the degradation of the extraembryonic serosal cuticle and the second embryonic cuticle (pro-nymphal cuticle) is essential for the development and molting of nymph from egg in Orthoptera <i<Locusta migratoria</i<. Chitinase 5 is an important gene for chitin degradation in nymphs and in the egg stage. In this study, we investigated the important roles of chitinase 5-1 (<i<LmCht5-1</i<) and chitinase 5-2 (<i<LmCht5-2</i<) in the degradation of the serosal and pro-nymphal cuticles during locust embryonic development. The serosal cuticle degrades from 7-day-old embryos (E7) to E13, along with the degradation of the pro-nymphal cuticle, which begins at E12 to E14. The mRNA and protein of <i<LmCht5-1</i< and <i<LmCht5-2</i< are expressed during the degradation process of the serosal cuticle and the pro-nymphal cuticle. RNAi experiments at the embryonic stage show that both ds<i<LmCht5-1</i< and ds<i<LmCht5-2</i< contribute to the failure of development in early and late embryogenesis. Further, during the serosal cuticle molting process, ultra-structure analysis indicated that ds<i<LmCht5-1</i< prevented the loss of the coarse chitin layer in the upper part in both early and late embryogenesis. Meanwhile, ds<i<LmCht5-2</i< blocked the degradation of the lower fine chitin layer at the early stage and blocked the chitin degradation of loose coarse chitin in the late molting process. During the degradation of the pro-nymphal cuticle, ds<i<LmCht5-1</i< suppresses chitin degradation between layers in the procuticle, while ds<i<LmCht5-2</i< suppresses chitin degradation into filaments inside of the layer. In summary, our results suggest that both <i<LmCht5-1</i< and <i<LmCht5-2</i< contribute to the degradation of the serosal and pro-nymphal cuticles during the locust embryonic stage. <i<Locust migratoria</i< serosal cuticle pro-nymphal cuticle Chitinase 5-1 Chitinase 5-2 Biology (General) Yanjun Huo verfasserin aut Qing Dong verfasserin aut Weiwei Liu verfasserin aut Lu Gao verfasserin aut Jiannan Zhou verfasserin aut Daqi Li verfasserin aut Xueyao Zhang verfasserin aut Jianzhen Zhang verfasserin aut Min Zhang verfasserin aut In Biology MDPI AG, 2012 11(2022), 12, p 1778 (DE-627)718622073 (DE-600)2661517-4 20797737 nnns volume:11 year:2022 number:12, p 1778 https://doi.org/10.3390/biology11121778 kostenfrei https://doaj.org/article/50f35e3834914158988062cfb21745a0 kostenfrei https://www.mdpi.com/2079-7737/11/12/1778 kostenfrei https://doaj.org/toc/2079-7737 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 12, p 1778
allfieldsGer	10.3390/biology11121778 doi (DE-627)DOAJ083228829 (DE-599)DOAJ50f35e3834914158988062cfb21745a0 DE-627 ger DE-627 rakwb eng QH301-705.5 Tingting Zhang verfasserin aut <i<LmCht5-1</i< and <i<LmCht5-2</i< Promote the Degradation of Serosal and Pro-Nymphal Cuticles during Locust Embryonic Development 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The success of the degradation of the extraembryonic serosal cuticle and the second embryonic cuticle (pro-nymphal cuticle) is essential for the development and molting of nymph from egg in Orthoptera <i<Locusta migratoria</i<. Chitinase 5 is an important gene for chitin degradation in nymphs and in the egg stage. In this study, we investigated the important roles of chitinase 5-1 (<i<LmCht5-1</i<) and chitinase 5-2 (<i<LmCht5-2</i<) in the degradation of the serosal and pro-nymphal cuticles during locust embryonic development. The serosal cuticle degrades from 7-day-old embryos (E7) to E13, along with the degradation of the pro-nymphal cuticle, which begins at E12 to E14. The mRNA and protein of <i<LmCht5-1</i< and <i<LmCht5-2</i< are expressed during the degradation process of the serosal cuticle and the pro-nymphal cuticle. RNAi experiments at the embryonic stage show that both ds<i<LmCht5-1</i< and ds<i<LmCht5-2</i< contribute to the failure of development in early and late embryogenesis. Further, during the serosal cuticle molting process, ultra-structure analysis indicated that ds<i<LmCht5-1</i< prevented the loss of the coarse chitin layer in the upper part in both early and late embryogenesis. Meanwhile, ds<i<LmCht5-2</i< blocked the degradation of the lower fine chitin layer at the early stage and blocked the chitin degradation of loose coarse chitin in the late molting process. During the degradation of the pro-nymphal cuticle, ds<i<LmCht5-1</i< suppresses chitin degradation between layers in the procuticle, while ds<i<LmCht5-2</i< suppresses chitin degradation into filaments inside of the layer. In summary, our results suggest that both <i<LmCht5-1</i< and <i<LmCht5-2</i< contribute to the degradation of the serosal and pro-nymphal cuticles during the locust embryonic stage. <i<Locust migratoria</i< serosal cuticle pro-nymphal cuticle Chitinase 5-1 Chitinase 5-2 Biology (General) Yanjun Huo verfasserin aut Qing Dong verfasserin aut Weiwei Liu verfasserin aut Lu Gao verfasserin aut Jiannan Zhou verfasserin aut Daqi Li verfasserin aut Xueyao Zhang verfasserin aut Jianzhen Zhang verfasserin aut Min Zhang verfasserin aut In Biology MDPI AG, 2012 11(2022), 12, p 1778 (DE-627)718622073 (DE-600)2661517-4 20797737 nnns volume:11 year:2022 number:12, p 1778 https://doi.org/10.3390/biology11121778 kostenfrei https://doaj.org/article/50f35e3834914158988062cfb21745a0 kostenfrei https://www.mdpi.com/2079-7737/11/12/1778 kostenfrei https://doaj.org/toc/2079-7737 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 12, p 1778
allfieldsSound	10.3390/biology11121778 doi (DE-627)DOAJ083228829 (DE-599)DOAJ50f35e3834914158988062cfb21745a0 DE-627 ger DE-627 rakwb eng QH301-705.5 Tingting Zhang verfasserin aut <i<LmCht5-1</i< and <i<LmCht5-2</i< Promote the Degradation of Serosal and Pro-Nymphal Cuticles during Locust Embryonic Development 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The success of the degradation of the extraembryonic serosal cuticle and the second embryonic cuticle (pro-nymphal cuticle) is essential for the development and molting of nymph from egg in Orthoptera <i<Locusta migratoria</i<. Chitinase 5 is an important gene for chitin degradation in nymphs and in the egg stage. In this study, we investigated the important roles of chitinase 5-1 (<i<LmCht5-1</i<) and chitinase 5-2 (<i<LmCht5-2</i<) in the degradation of the serosal and pro-nymphal cuticles during locust embryonic development. The serosal cuticle degrades from 7-day-old embryos (E7) to E13, along with the degradation of the pro-nymphal cuticle, which begins at E12 to E14. The mRNA and protein of <i<LmCht5-1</i< and <i<LmCht5-2</i< are expressed during the degradation process of the serosal cuticle and the pro-nymphal cuticle. RNAi experiments at the embryonic stage show that both ds<i<LmCht5-1</i< and ds<i<LmCht5-2</i< contribute to the failure of development in early and late embryogenesis. Further, during the serosal cuticle molting process, ultra-structure analysis indicated that ds<i<LmCht5-1</i< prevented the loss of the coarse chitin layer in the upper part in both early and late embryogenesis. Meanwhile, ds<i<LmCht5-2</i< blocked the degradation of the lower fine chitin layer at the early stage and blocked the chitin degradation of loose coarse chitin in the late molting process. During the degradation of the pro-nymphal cuticle, ds<i<LmCht5-1</i< suppresses chitin degradation between layers in the procuticle, while ds<i<LmCht5-2</i< suppresses chitin degradation into filaments inside of the layer. In summary, our results suggest that both <i<LmCht5-1</i< and <i<LmCht5-2</i< contribute to the degradation of the serosal and pro-nymphal cuticles during the locust embryonic stage. <i<Locust migratoria</i< serosal cuticle pro-nymphal cuticle Chitinase 5-1 Chitinase 5-2 Biology (General) Yanjun Huo verfasserin aut Qing Dong verfasserin aut Weiwei Liu verfasserin aut Lu Gao verfasserin aut Jiannan Zhou verfasserin aut Daqi Li verfasserin aut Xueyao Zhang verfasserin aut Jianzhen Zhang verfasserin aut Min Zhang verfasserin aut In Biology MDPI AG, 2012 11(2022), 12, p 1778 (DE-627)718622073 (DE-600)2661517-4 20797737 nnns volume:11 year:2022 number:12, p 1778 https://doi.org/10.3390/biology11121778 kostenfrei https://doaj.org/article/50f35e3834914158988062cfb21745a0 kostenfrei https://www.mdpi.com/2079-7737/11/12/1778 kostenfrei https://doaj.org/toc/2079-7737 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 12, p 1778
language	English
source	In Biology 11(2022), 12, p 1778 volume:11 year:2022 number:12, p 1778
sourceStr	In Biology 11(2022), 12, p 1778 volume:11 year:2022 number:12, p 1778
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<Locust migratoria</i< serosal cuticle pro-nymphal cuticle Chitinase 5-1 Chitinase 5-2 Biology (General)
isfreeaccess_bool	true
container_title	Biology
authorswithroles_txt_mv	Tingting Zhang @@aut@@ Yanjun Huo @@aut@@ Qing Dong @@aut@@ Weiwei Liu @@aut@@ Lu Gao @@aut@@ Jiannan Zhou @@aut@@ Daqi Li @@aut@@ Xueyao Zhang @@aut@@ Jianzhen Zhang @@aut@@ Min Zhang @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	718622073
id	DOAJ083228829
language_de	englisch
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Chitinase 5 is an important gene for chitin degradation in nymphs and in the egg stage. In this study, we investigated the important roles of chitinase 5-1 (<i<LmCht5-1</i<) and chitinase 5-2 (<i<LmCht5-2</i<) in the degradation of the serosal and pro-nymphal cuticles during locust embryonic development. The serosal cuticle degrades from 7-day-old embryos (E7) to E13, along with the degradation of the pro-nymphal cuticle, which begins at E12 to E14. The mRNA and protein of <i<LmCht5-1</i< and <i<LmCht5-2</i< are expressed during the degradation process of the serosal cuticle and the pro-nymphal cuticle. RNAi experiments at the embryonic stage show that both ds<i<LmCht5-1</i< and ds<i<LmCht5-2</i< contribute to the failure of development in early and late embryogenesis. Further, during the serosal cuticle molting process, ultra-structure analysis indicated that ds<i<LmCht5-1</i< prevented the loss of the coarse chitin layer in the upper part in both early and late embryogenesis. 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callnumber-first	Q - Science
author	Tingting Zhang
spellingShingle	Tingting Zhang misc QH301-705.5 misc <i<Locust migratoria</i< misc serosal cuticle misc pro-nymphal cuticle misc Chitinase 5-1 misc Chitinase 5-2 misc Biology (General) <i<LmCht5-1</i< and <i<LmCht5-2</i< Promote the Degradation of Serosal and Pro-Nymphal Cuticles during Locust Embryonic Development
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topic_title	QH301-705.5 <i<LmCht5-1</i< and <i<LmCht5-2</i< Promote the Degradation of Serosal and Pro-Nymphal Cuticles during Locust Embryonic Development <i<Locust migratoria</i< serosal cuticle pro-nymphal cuticle Chitinase 5-1 Chitinase 5-2
topic	misc QH301-705.5 misc <i<Locust migratoria</i< misc serosal cuticle misc pro-nymphal cuticle misc Chitinase 5-1 misc Chitinase 5-2 misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc <i<Locust migratoria</i< misc serosal cuticle misc pro-nymphal cuticle misc Chitinase 5-1 misc Chitinase 5-2 misc Biology (General)
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title	<i<LmCht5-1</i< and <i<LmCht5-2</i< Promote the Degradation of Serosal and Pro-Nymphal Cuticles during Locust Embryonic Development
ctrlnum	(DE-627)DOAJ083228829 (DE-599)DOAJ50f35e3834914158988062cfb21745a0
title_full	<i<LmCht5-1</i< and <i<LmCht5-2</i< Promote the Degradation of Serosal and Pro-Nymphal Cuticles during Locust Embryonic Development
author_sort	Tingting Zhang
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author_browse	Tingting Zhang Yanjun Huo Qing Dong Weiwei Liu Lu Gao Jiannan Zhou Daqi Li Xueyao Zhang Jianzhen Zhang Min Zhang
container_volume	11
class	QH301-705.5
format_se	Elektronische Aufsätze
author-letter	Tingting Zhang
doi_str_mv	10.3390/biology11121778
author2-role	verfasserin
title_sort	<i<lmcht5-1</i< and <i<lmcht5-2</i< promote the degradation of serosal and pro-nymphal cuticles during locust embryonic development
callnumber	QH301-705.5
title_auth	<i<LmCht5-1</i< and <i<LmCht5-2</i< Promote the Degradation of Serosal and Pro-Nymphal Cuticles during Locust Embryonic Development
abstract	The success of the degradation of the extraembryonic serosal cuticle and the second embryonic cuticle (pro-nymphal cuticle) is essential for the development and molting of nymph from egg in Orthoptera <i<Locusta migratoria</i<. Chitinase 5 is an important gene for chitin degradation in nymphs and in the egg stage. In this study, we investigated the important roles of chitinase 5-1 (<i<LmCht5-1</i<) and chitinase 5-2 (<i<LmCht5-2</i<) in the degradation of the serosal and pro-nymphal cuticles during locust embryonic development. The serosal cuticle degrades from 7-day-old embryos (E7) to E13, along with the degradation of the pro-nymphal cuticle, which begins at E12 to E14. The mRNA and protein of <i<LmCht5-1</i< and <i<LmCht5-2</i< are expressed during the degradation process of the serosal cuticle and the pro-nymphal cuticle. RNAi experiments at the embryonic stage show that both ds<i<LmCht5-1</i< and ds<i<LmCht5-2</i< contribute to the failure of development in early and late embryogenesis. Further, during the serosal cuticle molting process, ultra-structure analysis indicated that ds<i<LmCht5-1</i< prevented the loss of the coarse chitin layer in the upper part in both early and late embryogenesis. Meanwhile, ds<i<LmCht5-2</i< blocked the degradation of the lower fine chitin layer at the early stage and blocked the chitin degradation of loose coarse chitin in the late molting process. During the degradation of the pro-nymphal cuticle, ds<i<LmCht5-1</i< suppresses chitin degradation between layers in the procuticle, while ds<i<LmCht5-2</i< suppresses chitin degradation into filaments inside of the layer. In summary, our results suggest that both <i<LmCht5-1</i< and <i<LmCht5-2</i< contribute to the degradation of the serosal and pro-nymphal cuticles during the locust embryonic stage.
abstractGer	The success of the degradation of the extraembryonic serosal cuticle and the second embryonic cuticle (pro-nymphal cuticle) is essential for the development and molting of nymph from egg in Orthoptera <i<Locusta migratoria</i<. Chitinase 5 is an important gene for chitin degradation in nymphs and in the egg stage. In this study, we investigated the important roles of chitinase 5-1 (<i<LmCht5-1</i<) and chitinase 5-2 (<i<LmCht5-2</i<) in the degradation of the serosal and pro-nymphal cuticles during locust embryonic development. The serosal cuticle degrades from 7-day-old embryos (E7) to E13, along with the degradation of the pro-nymphal cuticle, which begins at E12 to E14. The mRNA and protein of <i<LmCht5-1</i< and <i<LmCht5-2</i< are expressed during the degradation process of the serosal cuticle and the pro-nymphal cuticle. RNAi experiments at the embryonic stage show that both ds<i<LmCht5-1</i< and ds<i<LmCht5-2</i< contribute to the failure of development in early and late embryogenesis. Further, during the serosal cuticle molting process, ultra-structure analysis indicated that ds<i<LmCht5-1</i< prevented the loss of the coarse chitin layer in the upper part in both early and late embryogenesis. Meanwhile, ds<i<LmCht5-2</i< blocked the degradation of the lower fine chitin layer at the early stage and blocked the chitin degradation of loose coarse chitin in the late molting process. During the degradation of the pro-nymphal cuticle, ds<i<LmCht5-1</i< suppresses chitin degradation between layers in the procuticle, while ds<i<LmCht5-2</i< suppresses chitin degradation into filaments inside of the layer. In summary, our results suggest that both <i<LmCht5-1</i< and <i<LmCht5-2</i< contribute to the degradation of the serosal and pro-nymphal cuticles during the locust embryonic stage.
abstract_unstemmed	The success of the degradation of the extraembryonic serosal cuticle and the second embryonic cuticle (pro-nymphal cuticle) is essential for the development and molting of nymph from egg in Orthoptera <i<Locusta migratoria</i<. Chitinase 5 is an important gene for chitin degradation in nymphs and in the egg stage. In this study, we investigated the important roles of chitinase 5-1 (<i<LmCht5-1</i<) and chitinase 5-2 (<i<LmCht5-2</i<) in the degradation of the serosal and pro-nymphal cuticles during locust embryonic development. The serosal cuticle degrades from 7-day-old embryos (E7) to E13, along with the degradation of the pro-nymphal cuticle, which begins at E12 to E14. The mRNA and protein of <i<LmCht5-1</i< and <i<LmCht5-2</i< are expressed during the degradation process of the serosal cuticle and the pro-nymphal cuticle. RNAi experiments at the embryonic stage show that both ds<i<LmCht5-1</i< and ds<i<LmCht5-2</i< contribute to the failure of development in early and late embryogenesis. Further, during the serosal cuticle molting process, ultra-structure analysis indicated that ds<i<LmCht5-1</i< prevented the loss of the coarse chitin layer in the upper part in both early and late embryogenesis. Meanwhile, ds<i<LmCht5-2</i< blocked the degradation of the lower fine chitin layer at the early stage and blocked the chitin degradation of loose coarse chitin in the late molting process. During the degradation of the pro-nymphal cuticle, ds<i<LmCht5-1</i< suppresses chitin degradation between layers in the procuticle, while ds<i<LmCht5-2</i< suppresses chitin degradation into filaments inside of the layer. In summary, our results suggest that both <i<LmCht5-1</i< and <i<LmCht5-2</i< contribute to the degradation of the serosal and pro-nymphal cuticles during the locust embryonic stage.
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title_short	<i<LmCht5-1</i< and <i<LmCht5-2</i< Promote the Degradation of Serosal and Pro-Nymphal Cuticles during Locust Embryonic Development
url	https://doi.org/10.3390/biology11121778 https://doaj.org/article/50f35e3834914158988062cfb21745a0 https://www.mdpi.com/2079-7737/11/12/1778 https://doaj.org/toc/2079-7737
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<i<LmCht5-1</i< and <i<LmCht5-2</i< Promote the Degradation of Serosal and Pro-Nymphal Cuticles during Locust Embryonic Development

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