Direct Phasing of Coiled-Coil Protein Crystals

Coiled-coil proteins consisting of multiple copies of helices take part in transmembrane transportation and oligomerization, and are used for drug delivery. Cross-alpha amyloid-like coiled-coil structures, in which tens of short helices align perpendicular to the fibril axis, often resist molecular...
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Autor*in:	Ruijiang Fu [verfasserIn] Wu-Pei Su [verfasserIn] Hongxing He [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	coiled coil ab initio phasing protein crystallography hybrid-input output non-crystallographic symmetry

Übergeordnetes Werk:	In: Crystals - MDPI AG, 2011, 12(2022), 11, p 1674
Übergeordnetes Werk:	volume:12 ; year:2022 ; number:11, p 1674

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/cryst12111674

Katalog-ID:	DOAJ08349121X

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520			\|a Coiled-coil proteins consisting of multiple copies of helices take part in transmembrane transportation and oligomerization, and are used for drug delivery. Cross-alpha amyloid-like coiled-coil structures, in which tens of short helices align perpendicular to the fibril axis, often resist molecular replacement due to the uncertainty to position each helix. Eight coiled-coil structures already solved and posted in the protein data bank are reconstructed ab initio to demonstrate the direct phasing results. Non-crystallographic symmetry and intermediate-resolution diffraction data are considered for direct phasing. The retrieved phases have a mean phase error around 30∼40°. The calculated density map is ready for model building, and the reconstructed model agrees with the deposited structure. The results indicate that direct phasing is an efficient approach to construct the protein envelope from scratch, build each helix without model bias which is also used to confirm the prediction of AlphaFold and RosettaFold, and solve the whole structure of coiled-coil proteins.
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spelling	10.3390/cryst12111674 doi (DE-627)DOAJ08349121X (DE-599)DOAJc04e67a75c3b4261a16388e86d16c167 DE-627 ger DE-627 rakwb eng QD901-999 Ruijiang Fu verfasserin aut Direct Phasing of Coiled-Coil Protein Crystals 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Coiled-coil proteins consisting of multiple copies of helices take part in transmembrane transportation and oligomerization, and are used for drug delivery. Cross-alpha amyloid-like coiled-coil structures, in which tens of short helices align perpendicular to the fibril axis, often resist molecular replacement due to the uncertainty to position each helix. Eight coiled-coil structures already solved and posted in the protein data bank are reconstructed ab initio to demonstrate the direct phasing results. Non-crystallographic symmetry and intermediate-resolution diffraction data are considered for direct phasing. The retrieved phases have a mean phase error around 30∼40°. The calculated density map is ready for model building, and the reconstructed model agrees with the deposited structure. The results indicate that direct phasing is an efficient approach to construct the protein envelope from scratch, build each helix without model bias which is also used to confirm the prediction of AlphaFold and RosettaFold, and solve the whole structure of coiled-coil proteins. coiled coil ab initio phasing protein crystallography hybrid-input output non-crystallographic symmetry Crystallography Wu-Pei Su verfasserin aut Hongxing He verfasserin aut In Crystals MDPI AG, 2011 12(2022), 11, p 1674 (DE-627)718303067 (DE-600)2661516-2 20734352 nnns volume:12 year:2022 number:11, p 1674 https://doi.org/10.3390/cryst12111674 kostenfrei https://doaj.org/article/c04e67a75c3b4261a16388e86d16c167 kostenfrei https://www.mdpi.com/2073-4352/12/11/1674 kostenfrei https://doaj.org/toc/2073-4352 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 11, p 1674
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allfieldsGer	10.3390/cryst12111674 doi (DE-627)DOAJ08349121X (DE-599)DOAJc04e67a75c3b4261a16388e86d16c167 DE-627 ger DE-627 rakwb eng QD901-999 Ruijiang Fu verfasserin aut Direct Phasing of Coiled-Coil Protein Crystals 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Coiled-coil proteins consisting of multiple copies of helices take part in transmembrane transportation and oligomerization, and are used for drug delivery. Cross-alpha amyloid-like coiled-coil structures, in which tens of short helices align perpendicular to the fibril axis, often resist molecular replacement due to the uncertainty to position each helix. Eight coiled-coil structures already solved and posted in the protein data bank are reconstructed ab initio to demonstrate the direct phasing results. Non-crystallographic symmetry and intermediate-resolution diffraction data are considered for direct phasing. The retrieved phases have a mean phase error around 30∼40°. The calculated density map is ready for model building, and the reconstructed model agrees with the deposited structure. The results indicate that direct phasing is an efficient approach to construct the protein envelope from scratch, build each helix without model bias which is also used to confirm the prediction of AlphaFold and RosettaFold, and solve the whole structure of coiled-coil proteins. coiled coil ab initio phasing protein crystallography hybrid-input output non-crystallographic symmetry Crystallography Wu-Pei Su verfasserin aut Hongxing He verfasserin aut In Crystals MDPI AG, 2011 12(2022), 11, p 1674 (DE-627)718303067 (DE-600)2661516-2 20734352 nnns volume:12 year:2022 number:11, p 1674 https://doi.org/10.3390/cryst12111674 kostenfrei https://doaj.org/article/c04e67a75c3b4261a16388e86d16c167 kostenfrei https://www.mdpi.com/2073-4352/12/11/1674 kostenfrei https://doaj.org/toc/2073-4352 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 11, p 1674
allfieldsSound	10.3390/cryst12111674 doi (DE-627)DOAJ08349121X (DE-599)DOAJc04e67a75c3b4261a16388e86d16c167 DE-627 ger DE-627 rakwb eng QD901-999 Ruijiang Fu verfasserin aut Direct Phasing of Coiled-Coil Protein Crystals 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Coiled-coil proteins consisting of multiple copies of helices take part in transmembrane transportation and oligomerization, and are used for drug delivery. Cross-alpha amyloid-like coiled-coil structures, in which tens of short helices align perpendicular to the fibril axis, often resist molecular replacement due to the uncertainty to position each helix. Eight coiled-coil structures already solved and posted in the protein data bank are reconstructed ab initio to demonstrate the direct phasing results. Non-crystallographic symmetry and intermediate-resolution diffraction data are considered for direct phasing. The retrieved phases have a mean phase error around 30∼40°. The calculated density map is ready for model building, and the reconstructed model agrees with the deposited structure. The results indicate that direct phasing is an efficient approach to construct the protein envelope from scratch, build each helix without model bias which is also used to confirm the prediction of AlphaFold and RosettaFold, and solve the whole structure of coiled-coil proteins. coiled coil ab initio phasing protein crystallography hybrid-input output non-crystallographic symmetry Crystallography Wu-Pei Su verfasserin aut Hongxing He verfasserin aut In Crystals MDPI AG, 2011 12(2022), 11, p 1674 (DE-627)718303067 (DE-600)2661516-2 20734352 nnns volume:12 year:2022 number:11, p 1674 https://doi.org/10.3390/cryst12111674 kostenfrei https://doaj.org/article/c04e67a75c3b4261a16388e86d16c167 kostenfrei https://www.mdpi.com/2073-4352/12/11/1674 kostenfrei https://doaj.org/toc/2073-4352 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 11, p 1674
language	English
source	In Crystals 12(2022), 11, p 1674 volume:12 year:2022 number:11, p 1674
sourceStr	In Crystals 12(2022), 11, p 1674 volume:12 year:2022 number:11, p 1674
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topic_facet	coiled coil ab initio phasing protein crystallography hybrid-input output non-crystallographic symmetry Crystallography
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container_title	Crystals
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callnumber-first	Q - Science
author	Ruijiang Fu
spellingShingle	Ruijiang Fu misc QD901-999 misc coiled coil misc ab initio phasing misc protein crystallography misc hybrid-input output misc non-crystallographic symmetry misc Crystallography Direct Phasing of Coiled-Coil Protein Crystals
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topic_title	QD901-999 Direct Phasing of Coiled-Coil Protein Crystals coiled coil ab initio phasing protein crystallography hybrid-input output non-crystallographic symmetry
topic	misc QD901-999 misc coiled coil misc ab initio phasing misc protein crystallography misc hybrid-input output misc non-crystallographic symmetry misc Crystallography
topic_unstemmed	misc QD901-999 misc coiled coil misc ab initio phasing misc protein crystallography misc hybrid-input output misc non-crystallographic symmetry misc Crystallography
topic_browse	misc QD901-999 misc coiled coil misc ab initio phasing misc protein crystallography misc hybrid-input output misc non-crystallographic symmetry misc Crystallography
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title	Direct Phasing of Coiled-Coil Protein Crystals
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title_full	Direct Phasing of Coiled-Coil Protein Crystals
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title_sort	direct phasing of coiled-coil protein crystals
callnumber	QD901-999
title_auth	Direct Phasing of Coiled-Coil Protein Crystals
abstract	Coiled-coil proteins consisting of multiple copies of helices take part in transmembrane transportation and oligomerization, and are used for drug delivery. Cross-alpha amyloid-like coiled-coil structures, in which tens of short helices align perpendicular to the fibril axis, often resist molecular replacement due to the uncertainty to position each helix. Eight coiled-coil structures already solved and posted in the protein data bank are reconstructed ab initio to demonstrate the direct phasing results. Non-crystallographic symmetry and intermediate-resolution diffraction data are considered for direct phasing. The retrieved phases have a mean phase error around 30∼40°. The calculated density map is ready for model building, and the reconstructed model agrees with the deposited structure. The results indicate that direct phasing is an efficient approach to construct the protein envelope from scratch, build each helix without model bias which is also used to confirm the prediction of AlphaFold and RosettaFold, and solve the whole structure of coiled-coil proteins.
abstractGer	Coiled-coil proteins consisting of multiple copies of helices take part in transmembrane transportation and oligomerization, and are used for drug delivery. Cross-alpha amyloid-like coiled-coil structures, in which tens of short helices align perpendicular to the fibril axis, often resist molecular replacement due to the uncertainty to position each helix. Eight coiled-coil structures already solved and posted in the protein data bank are reconstructed ab initio to demonstrate the direct phasing results. Non-crystallographic symmetry and intermediate-resolution diffraction data are considered for direct phasing. The retrieved phases have a mean phase error around 30∼40°. The calculated density map is ready for model building, and the reconstructed model agrees with the deposited structure. The results indicate that direct phasing is an efficient approach to construct the protein envelope from scratch, build each helix without model bias which is also used to confirm the prediction of AlphaFold and RosettaFold, and solve the whole structure of coiled-coil proteins.
abstract_unstemmed	Coiled-coil proteins consisting of multiple copies of helices take part in transmembrane transportation and oligomerization, and are used for drug delivery. Cross-alpha amyloid-like coiled-coil structures, in which tens of short helices align perpendicular to the fibril axis, often resist molecular replacement due to the uncertainty to position each helix. Eight coiled-coil structures already solved and posted in the protein data bank are reconstructed ab initio to demonstrate the direct phasing results. Non-crystallographic symmetry and intermediate-resolution diffraction data are considered for direct phasing. The retrieved phases have a mean phase error around 30∼40°. The calculated density map is ready for model building, and the reconstructed model agrees with the deposited structure. The results indicate that direct phasing is an efficient approach to construct the protein envelope from scratch, build each helix without model bias which is also used to confirm the prediction of AlphaFold and RosettaFold, and solve the whole structure of coiled-coil proteins.
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container_issue	11, p 1674
title_short	Direct Phasing of Coiled-Coil Protein Crystals
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up_date	2024-07-03T17:46:02.134Z
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Vorhandene Bände

Nicht das Richtige dabei?