Cold Tolerance of <i<ScCBL6</i< Is Associated with Tonoplast Transporters and Photosynthesis in <i<Arabidopsis</i<

Plants that are adapted to harsh environments offer enormous opportunity to understand stress responses in ecological systems. <i<Stipa capillacea</i< is widely distributed in the frigid and arid region of the Tibetan Plateau, but its signal transduction system under cold stress has not...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Yanli Zhou [verfasserIn] Jingling Zhang [verfasserIn] Changhong Zhao [verfasserIn] Guangqiang Long [verfasserIn] Chengli Zhou [verfasserIn] Xudong Sun [verfasserIn] Yunqiang Yang [verfasserIn] Chengjun Zhang [verfasserIn] Yongping Yang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	calcineurin B-like protein cold tolerance transcriptome tonoplast

Übergeordnetes Werk:	In: Current Issues in Molecular Biology - MDPI AG, 2021, 44(2022), 11, Seite 5579-5592
Übergeordnetes Werk:	volume:44 ; year:2022 ; number:11 ; pages:5579-5592

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/cimb44110378

Katalog-ID:	DOAJ083494065

Internformat


LEADER	01000caa a22002652 4500
001	DOAJ083494065
003	DE-627
005	20240414172756.0
007	cr uuu---uuuuu
008	230311s2022 xx \|\|\|\|\|o 00\| \|\|eng c
024	7		\|a 10.3390/cimb44110378 \|2 doi
035			\|a (DE-627)DOAJ083494065
035			\|a (DE-599)DOAJ87ee7bb0ed2f485aaa52ddb8ec074b65
040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a eng
050		0	\|a QH301-705.5
100	0		\|a Yanli Zhou \|e verfasserin \|4 aut
245	1	0	\|a Cold Tolerance of <i<ScCBL6</i< Is Associated with Tonoplast Transporters and Photosynthesis in <i<Arabidopsis</i<
264		1	\|c 2022
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
520			\|a Plants that are adapted to harsh environments offer enormous opportunity to understand stress responses in ecological systems. <i<Stipa capillacea</i< is widely distributed in the frigid and arid region of the Tibetan Plateau, but its signal transduction system under cold stress has not been characterized. In this study, we isolated a cDNA encoding the signal transduction protein, ScCBL6, from <i<S</i<. <i<capillacea</i<, and evaluated its role in cold tolerance by ectopically expressing it in <i<Arabidopsis</i<. Full-length <i<ScCBL6</i< encode 227 amino acids, and are clustered with CBL6 in <i<Stipa purpurea</i< and <i<Oryza sativa</i< in a phylogenetic analysis. Compared with tolerance in wild-type (WT) plants, ScCBL6-overexpressing plants (<i<ScCBL6-OXP</i<) were more tolerant to cold stress but not to drought stress, as confirmed by their high photosynthetic capacity (Fv/Fm) and survival rate under cold stress. We further compared their cold-responsive transcriptome profiles by RNA sequencing. In total, 3931 genes were differentially expressed by the introduction of <i<ScCBL6</i<. These gene products were involved in multiple processes such as the immune system, lipid catabolism, and secondary metabolism. A KEGG pathway analysis revealed that they were mainly enriched in plant hormone signal transduction and biomacromolecule metabolism. Proteins encoded by differentially expressed genes were predicted to be localized in chloroplasts, mitochondria, and vacuoles, suggesting that ScCBL6 exerts a wide range of functions. Based on its tonoplast subcellular location combined with integrated transcriptome and physiological analyses of <i<ScCBL6-OXP</i<, we inferred that ScCBL6 improves plant cold stress tolerance in <i<Arabidopsis</i< via the regulation of photosynthesis, redox status, and tonoplast metabolite transporters.
650		4	\|a calcineurin B-like protein
650		4	\|a cold tolerance
650		4	\|a <i<Stipa capillacea</i<
650		4	\|a transcriptome
650		4	\|a tonoplast
653		0	\|a Biology (General)
700	0		\|a Jingling Zhang \|e verfasserin \|4 aut
700	0		\|a Changhong Zhao \|e verfasserin \|4 aut
700	0		\|a Guangqiang Long \|e verfasserin \|4 aut
700	0		\|a Chengli Zhou \|e verfasserin \|4 aut
700	0		\|a Xudong Sun \|e verfasserin \|4 aut
700	0		\|a Yunqiang Yang \|e verfasserin \|4 aut
700	0		\|a Chengjun Zhang \|e verfasserin \|4 aut
700	0		\|a Yongping Yang \|e verfasserin \|4 aut
773	0	8	\|i In \|t Current Issues in Molecular Biology \|d MDPI AG, 2021 \|g 44(2022), 11, Seite 5579-5592 \|w (DE-627)355690365 \|w (DE-600)2090836-2 \|x 14673045 \|7 nnns
773	1	8	\|g volume:44 \|g year:2022 \|g number:11 \|g pages:5579-5592
856	4	0	\|u https://doi.org/10.3390/cimb44110378 \|z kostenfrei
856	4	0	\|u https://doaj.org/article/87ee7bb0ed2f485aaa52ddb8ec074b65 \|z kostenfrei
856	4	0	\|u https://www.mdpi.com/1467-3045/44/11/378 \|z kostenfrei
856	4	2	\|u https://doaj.org/toc/1467-3037 \|y Journal toc \|z kostenfrei
856	4	2	\|u https://doaj.org/toc/1467-3045 \|y Journal toc \|z kostenfrei
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_DOAJ
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_95
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_213
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 44 \|j 2022 \|e 11 \|h 5579-5592

Indexfelder

author_variant	y z yz j z jz c z cz g l gl c z cz x s xs y y yy c z cz y y yy
matchkey_str	article:14673045:2022----::odoeacoicb6iascaewttnpatrnpresnpoo
hierarchy_sort_str	2022
callnumber-subject-code	QH
publishDate	2022
allfields	10.3390/cimb44110378 doi (DE-627)DOAJ083494065 (DE-599)DOAJ87ee7bb0ed2f485aaa52ddb8ec074b65 DE-627 ger DE-627 rakwb eng QH301-705.5 Yanli Zhou verfasserin aut Cold Tolerance of <i<ScCBL6</i< Is Associated with Tonoplast Transporters and Photosynthesis in <i<Arabidopsis</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plants that are adapted to harsh environments offer enormous opportunity to understand stress responses in ecological systems. <i<Stipa capillacea</i< is widely distributed in the frigid and arid region of the Tibetan Plateau, but its signal transduction system under cold stress has not been characterized. In this study, we isolated a cDNA encoding the signal transduction protein, ScCBL6, from <i<S</i<. <i<capillacea</i<, and evaluated its role in cold tolerance by ectopically expressing it in <i<Arabidopsis</i<. Full-length <i<ScCBL6</i< encode 227 amino acids, and are clustered with CBL6 in <i<Stipa purpurea</i< and <i<Oryza sativa</i< in a phylogenetic analysis. Compared with tolerance in wild-type (WT) plants, ScCBL6-overexpressing plants (<i<ScCBL6-OXP</i<) were more tolerant to cold stress but not to drought stress, as confirmed by their high photosynthetic capacity (Fv/Fm) and survival rate under cold stress. We further compared their cold-responsive transcriptome profiles by RNA sequencing. In total, 3931 genes were differentially expressed by the introduction of <i<ScCBL6</i<. These gene products were involved in multiple processes such as the immune system, lipid catabolism, and secondary metabolism. A KEGG pathway analysis revealed that they were mainly enriched in plant hormone signal transduction and biomacromolecule metabolism. Proteins encoded by differentially expressed genes were predicted to be localized in chloroplasts, mitochondria, and vacuoles, suggesting that ScCBL6 exerts a wide range of functions. Based on its tonoplast subcellular location combined with integrated transcriptome and physiological analyses of <i<ScCBL6-OXP</i<, we inferred that ScCBL6 improves plant cold stress tolerance in <i<Arabidopsis</i< via the regulation of photosynthesis, redox status, and tonoplast metabolite transporters. calcineurin B-like protein cold tolerance <i<Stipa capillacea</i< transcriptome tonoplast Biology (General) Jingling Zhang verfasserin aut Changhong Zhao verfasserin aut Guangqiang Long verfasserin aut Chengli Zhou verfasserin aut Xudong Sun verfasserin aut Yunqiang Yang verfasserin aut Chengjun Zhang verfasserin aut Yongping Yang verfasserin aut In Current Issues in Molecular Biology MDPI AG, 2021 44(2022), 11, Seite 5579-5592 (DE-627)355690365 (DE-600)2090836-2 14673045 nnns volume:44 year:2022 number:11 pages:5579-5592 https://doi.org/10.3390/cimb44110378 kostenfrei https://doaj.org/article/87ee7bb0ed2f485aaa52ddb8ec074b65 kostenfrei https://www.mdpi.com/1467-3045/44/11/378 kostenfrei https://doaj.org/toc/1467-3037 Journal toc kostenfrei https://doaj.org/toc/1467-3045 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 44 2022 11 5579-5592
spelling	10.3390/cimb44110378 doi (DE-627)DOAJ083494065 (DE-599)DOAJ87ee7bb0ed2f485aaa52ddb8ec074b65 DE-627 ger DE-627 rakwb eng QH301-705.5 Yanli Zhou verfasserin aut Cold Tolerance of <i<ScCBL6</i< Is Associated with Tonoplast Transporters and Photosynthesis in <i<Arabidopsis</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plants that are adapted to harsh environments offer enormous opportunity to understand stress responses in ecological systems. <i<Stipa capillacea</i< is widely distributed in the frigid and arid region of the Tibetan Plateau, but its signal transduction system under cold stress has not been characterized. In this study, we isolated a cDNA encoding the signal transduction protein, ScCBL6, from <i<S</i<. <i<capillacea</i<, and evaluated its role in cold tolerance by ectopically expressing it in <i<Arabidopsis</i<. Full-length <i<ScCBL6</i< encode 227 amino acids, and are clustered with CBL6 in <i<Stipa purpurea</i< and <i<Oryza sativa</i< in a phylogenetic analysis. Compared with tolerance in wild-type (WT) plants, ScCBL6-overexpressing plants (<i<ScCBL6-OXP</i<) were more tolerant to cold stress but not to drought stress, as confirmed by their high photosynthetic capacity (Fv/Fm) and survival rate under cold stress. We further compared their cold-responsive transcriptome profiles by RNA sequencing. In total, 3931 genes were differentially expressed by the introduction of <i<ScCBL6</i<. These gene products were involved in multiple processes such as the immune system, lipid catabolism, and secondary metabolism. A KEGG pathway analysis revealed that they were mainly enriched in plant hormone signal transduction and biomacromolecule metabolism. Proteins encoded by differentially expressed genes were predicted to be localized in chloroplasts, mitochondria, and vacuoles, suggesting that ScCBL6 exerts a wide range of functions. Based on its tonoplast subcellular location combined with integrated transcriptome and physiological analyses of <i<ScCBL6-OXP</i<, we inferred that ScCBL6 improves plant cold stress tolerance in <i<Arabidopsis</i< via the regulation of photosynthesis, redox status, and tonoplast metabolite transporters. calcineurin B-like protein cold tolerance <i<Stipa capillacea</i< transcriptome tonoplast Biology (General) Jingling Zhang verfasserin aut Changhong Zhao verfasserin aut Guangqiang Long verfasserin aut Chengli Zhou verfasserin aut Xudong Sun verfasserin aut Yunqiang Yang verfasserin aut Chengjun Zhang verfasserin aut Yongping Yang verfasserin aut In Current Issues in Molecular Biology MDPI AG, 2021 44(2022), 11, Seite 5579-5592 (DE-627)355690365 (DE-600)2090836-2 14673045 nnns volume:44 year:2022 number:11 pages:5579-5592 https://doi.org/10.3390/cimb44110378 kostenfrei https://doaj.org/article/87ee7bb0ed2f485aaa52ddb8ec074b65 kostenfrei https://www.mdpi.com/1467-3045/44/11/378 kostenfrei https://doaj.org/toc/1467-3037 Journal toc kostenfrei https://doaj.org/toc/1467-3045 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 44 2022 11 5579-5592
allfields_unstemmed	10.3390/cimb44110378 doi (DE-627)DOAJ083494065 (DE-599)DOAJ87ee7bb0ed2f485aaa52ddb8ec074b65 DE-627 ger DE-627 rakwb eng QH301-705.5 Yanli Zhou verfasserin aut Cold Tolerance of <i<ScCBL6</i< Is Associated with Tonoplast Transporters and Photosynthesis in <i<Arabidopsis</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plants that are adapted to harsh environments offer enormous opportunity to understand stress responses in ecological systems. <i<Stipa capillacea</i< is widely distributed in the frigid and arid region of the Tibetan Plateau, but its signal transduction system under cold stress has not been characterized. In this study, we isolated a cDNA encoding the signal transduction protein, ScCBL6, from <i<S</i<. <i<capillacea</i<, and evaluated its role in cold tolerance by ectopically expressing it in <i<Arabidopsis</i<. Full-length <i<ScCBL6</i< encode 227 amino acids, and are clustered with CBL6 in <i<Stipa purpurea</i< and <i<Oryza sativa</i< in a phylogenetic analysis. Compared with tolerance in wild-type (WT) plants, ScCBL6-overexpressing plants (<i<ScCBL6-OXP</i<) were more tolerant to cold stress but not to drought stress, as confirmed by their high photosynthetic capacity (Fv/Fm) and survival rate under cold stress. We further compared their cold-responsive transcriptome profiles by RNA sequencing. In total, 3931 genes were differentially expressed by the introduction of <i<ScCBL6</i<. These gene products were involved in multiple processes such as the immune system, lipid catabolism, and secondary metabolism. A KEGG pathway analysis revealed that they were mainly enriched in plant hormone signal transduction and biomacromolecule metabolism. Proteins encoded by differentially expressed genes were predicted to be localized in chloroplasts, mitochondria, and vacuoles, suggesting that ScCBL6 exerts a wide range of functions. Based on its tonoplast subcellular location combined with integrated transcriptome and physiological analyses of <i<ScCBL6-OXP</i<, we inferred that ScCBL6 improves plant cold stress tolerance in <i<Arabidopsis</i< via the regulation of photosynthesis, redox status, and tonoplast metabolite transporters. calcineurin B-like protein cold tolerance <i<Stipa capillacea</i< transcriptome tonoplast Biology (General) Jingling Zhang verfasserin aut Changhong Zhao verfasserin aut Guangqiang Long verfasserin aut Chengli Zhou verfasserin aut Xudong Sun verfasserin aut Yunqiang Yang verfasserin aut Chengjun Zhang verfasserin aut Yongping Yang verfasserin aut In Current Issues in Molecular Biology MDPI AG, 2021 44(2022), 11, Seite 5579-5592 (DE-627)355690365 (DE-600)2090836-2 14673045 nnns volume:44 year:2022 number:11 pages:5579-5592 https://doi.org/10.3390/cimb44110378 kostenfrei https://doaj.org/article/87ee7bb0ed2f485aaa52ddb8ec074b65 kostenfrei https://www.mdpi.com/1467-3045/44/11/378 kostenfrei https://doaj.org/toc/1467-3037 Journal toc kostenfrei https://doaj.org/toc/1467-3045 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 44 2022 11 5579-5592
allfieldsGer	10.3390/cimb44110378 doi (DE-627)DOAJ083494065 (DE-599)DOAJ87ee7bb0ed2f485aaa52ddb8ec074b65 DE-627 ger DE-627 rakwb eng QH301-705.5 Yanli Zhou verfasserin aut Cold Tolerance of <i<ScCBL6</i< Is Associated with Tonoplast Transporters and Photosynthesis in <i<Arabidopsis</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plants that are adapted to harsh environments offer enormous opportunity to understand stress responses in ecological systems. <i<Stipa capillacea</i< is widely distributed in the frigid and arid region of the Tibetan Plateau, but its signal transduction system under cold stress has not been characterized. In this study, we isolated a cDNA encoding the signal transduction protein, ScCBL6, from <i<S</i<. <i<capillacea</i<, and evaluated its role in cold tolerance by ectopically expressing it in <i<Arabidopsis</i<. Full-length <i<ScCBL6</i< encode 227 amino acids, and are clustered with CBL6 in <i<Stipa purpurea</i< and <i<Oryza sativa</i< in a phylogenetic analysis. Compared with tolerance in wild-type (WT) plants, ScCBL6-overexpressing plants (<i<ScCBL6-OXP</i<) were more tolerant to cold stress but not to drought stress, as confirmed by their high photosynthetic capacity (Fv/Fm) and survival rate under cold stress. We further compared their cold-responsive transcriptome profiles by RNA sequencing. In total, 3931 genes were differentially expressed by the introduction of <i<ScCBL6</i<. These gene products were involved in multiple processes such as the immune system, lipid catabolism, and secondary metabolism. A KEGG pathway analysis revealed that they were mainly enriched in plant hormone signal transduction and biomacromolecule metabolism. Proteins encoded by differentially expressed genes were predicted to be localized in chloroplasts, mitochondria, and vacuoles, suggesting that ScCBL6 exerts a wide range of functions. Based on its tonoplast subcellular location combined with integrated transcriptome and physiological analyses of <i<ScCBL6-OXP</i<, we inferred that ScCBL6 improves plant cold stress tolerance in <i<Arabidopsis</i< via the regulation of photosynthesis, redox status, and tonoplast metabolite transporters. calcineurin B-like protein cold tolerance <i<Stipa capillacea</i< transcriptome tonoplast Biology (General) Jingling Zhang verfasserin aut Changhong Zhao verfasserin aut Guangqiang Long verfasserin aut Chengli Zhou verfasserin aut Xudong Sun verfasserin aut Yunqiang Yang verfasserin aut Chengjun Zhang verfasserin aut Yongping Yang verfasserin aut In Current Issues in Molecular Biology MDPI AG, 2021 44(2022), 11, Seite 5579-5592 (DE-627)355690365 (DE-600)2090836-2 14673045 nnns volume:44 year:2022 number:11 pages:5579-5592 https://doi.org/10.3390/cimb44110378 kostenfrei https://doaj.org/article/87ee7bb0ed2f485aaa52ddb8ec074b65 kostenfrei https://www.mdpi.com/1467-3045/44/11/378 kostenfrei https://doaj.org/toc/1467-3037 Journal toc kostenfrei https://doaj.org/toc/1467-3045 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 44 2022 11 5579-5592
allfieldsSound	10.3390/cimb44110378 doi (DE-627)DOAJ083494065 (DE-599)DOAJ87ee7bb0ed2f485aaa52ddb8ec074b65 DE-627 ger DE-627 rakwb eng QH301-705.5 Yanli Zhou verfasserin aut Cold Tolerance of <i<ScCBL6</i< Is Associated with Tonoplast Transporters and Photosynthesis in <i<Arabidopsis</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plants that are adapted to harsh environments offer enormous opportunity to understand stress responses in ecological systems. <i<Stipa capillacea</i< is widely distributed in the frigid and arid region of the Tibetan Plateau, but its signal transduction system under cold stress has not been characterized. In this study, we isolated a cDNA encoding the signal transduction protein, ScCBL6, from <i<S</i<. <i<capillacea</i<, and evaluated its role in cold tolerance by ectopically expressing it in <i<Arabidopsis</i<. Full-length <i<ScCBL6</i< encode 227 amino acids, and are clustered with CBL6 in <i<Stipa purpurea</i< and <i<Oryza sativa</i< in a phylogenetic analysis. Compared with tolerance in wild-type (WT) plants, ScCBL6-overexpressing plants (<i<ScCBL6-OXP</i<) were more tolerant to cold stress but not to drought stress, as confirmed by their high photosynthetic capacity (Fv/Fm) and survival rate under cold stress. We further compared their cold-responsive transcriptome profiles by RNA sequencing. In total, 3931 genes were differentially expressed by the introduction of <i<ScCBL6</i<. These gene products were involved in multiple processes such as the immune system, lipid catabolism, and secondary metabolism. A KEGG pathway analysis revealed that they were mainly enriched in plant hormone signal transduction and biomacromolecule metabolism. Proteins encoded by differentially expressed genes were predicted to be localized in chloroplasts, mitochondria, and vacuoles, suggesting that ScCBL6 exerts a wide range of functions. Based on its tonoplast subcellular location combined with integrated transcriptome and physiological analyses of <i<ScCBL6-OXP</i<, we inferred that ScCBL6 improves plant cold stress tolerance in <i<Arabidopsis</i< via the regulation of photosynthesis, redox status, and tonoplast metabolite transporters. calcineurin B-like protein cold tolerance <i<Stipa capillacea</i< transcriptome tonoplast Biology (General) Jingling Zhang verfasserin aut Changhong Zhao verfasserin aut Guangqiang Long verfasserin aut Chengli Zhou verfasserin aut Xudong Sun verfasserin aut Yunqiang Yang verfasserin aut Chengjun Zhang verfasserin aut Yongping Yang verfasserin aut In Current Issues in Molecular Biology MDPI AG, 2021 44(2022), 11, Seite 5579-5592 (DE-627)355690365 (DE-600)2090836-2 14673045 nnns volume:44 year:2022 number:11 pages:5579-5592 https://doi.org/10.3390/cimb44110378 kostenfrei https://doaj.org/article/87ee7bb0ed2f485aaa52ddb8ec074b65 kostenfrei https://www.mdpi.com/1467-3045/44/11/378 kostenfrei https://doaj.org/toc/1467-3037 Journal toc kostenfrei https://doaj.org/toc/1467-3045 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 44 2022 11 5579-5592
language	English
source	In Current Issues in Molecular Biology 44(2022), 11, Seite 5579-5592 volume:44 year:2022 number:11 pages:5579-5592
sourceStr	In Current Issues in Molecular Biology 44(2022), 11, Seite 5579-5592 volume:44 year:2022 number:11 pages:5579-5592
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	calcineurin B-like protein cold tolerance <i<Stipa capillacea</i< transcriptome tonoplast Biology (General)
isfreeaccess_bool	true
container_title	Current Issues in Molecular Biology
authorswithroles_txt_mv	Yanli Zhou @@aut@@ Jingling Zhang @@aut@@ Changhong Zhao @@aut@@ Guangqiang Long @@aut@@ Chengli Zhou @@aut@@ Xudong Sun @@aut@@ Yunqiang Yang @@aut@@ Chengjun Zhang @@aut@@ Yongping Yang @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	355690365
id	DOAJ083494065
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ083494065</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240414172756.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230311s2022 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3390/cimb44110378</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ083494065</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ87ee7bb0ed2f485aaa52ddb8ec074b65</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH301-705.5</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Yanli Zhou</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Cold Tolerance of <i<ScCBL6</i< Is Associated with Tonoplast Transporters and Photosynthesis in <i<Arabidopsis</i<</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2022</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Plants that are adapted to harsh environments offer enormous opportunity to understand stress responses in ecological systems. <i<Stipa capillacea</i< is widely distributed in the frigid and arid region of the Tibetan Plateau, but its signal transduction system under cold stress has not been characterized. In this study, we isolated a cDNA encoding the signal transduction protein, ScCBL6, from <i<S</i<. <i<capillacea</i<, and evaluated its role in cold tolerance by ectopically expressing it in <i<Arabidopsis</i<. Full-length <i<ScCBL6</i< encode 227 amino acids, and are clustered with CBL6 in <i<Stipa purpurea</i< and <i<Oryza sativa</i< in a phylogenetic analysis. Compared with tolerance in wild-type (WT) plants, ScCBL6-overexpressing plants (<i<ScCBL6-OXP</i<) were more tolerant to cold stress but not to drought stress, as confirmed by their high photosynthetic capacity (Fv/Fm) and survival rate under cold stress. We further compared their cold-responsive transcriptome profiles by RNA sequencing. In total, 3931 genes were differentially expressed by the introduction of <i<ScCBL6</i<. These gene products were involved in multiple processes such as the immune system, lipid catabolism, and secondary metabolism. A KEGG pathway analysis revealed that they were mainly enriched in plant hormone signal transduction and biomacromolecule metabolism. Proteins encoded by differentially expressed genes were predicted to be localized in chloroplasts, mitochondria, and vacuoles, suggesting that ScCBL6 exerts a wide range of functions. Based on its tonoplast subcellular location combined with integrated transcriptome and physiological analyses of <i<ScCBL6-OXP</i<, we inferred that ScCBL6 improves plant cold stress tolerance in <i<Arabidopsis</i< via the regulation of photosynthesis, redox status, and tonoplast metabolite transporters.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">calcineurin B-like protein</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">cold tolerance</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><i<Stipa capillacea</i<</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">transcriptome</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">tonoplast</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Biology (General)</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Jingling Zhang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Changhong Zhao</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Guangqiang Long</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Chengli Zhou</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Xudong Sun</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Yunqiang Yang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Chengjun Zhang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Yongping Yang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Current Issues in Molecular Biology</subfield><subfield code="d">MDPI AG, 2021</subfield><subfield code="g">44(2022), 11, Seite 5579-5592</subfield><subfield code="w">(DE-627)355690365</subfield><subfield code="w">(DE-600)2090836-2</subfield><subfield code="x">14673045</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:44</subfield><subfield code="g">year:2022</subfield><subfield code="g">number:11</subfield><subfield code="g">pages:5579-5592</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.3390/cimb44110378</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/87ee7bb0ed2f485aaa52ddb8ec074b65</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.mdpi.com/1467-3045/44/11/378</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1467-3037</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1467-3045</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">44</subfield><subfield code="j">2022</subfield><subfield code="e">11</subfield><subfield code="h">5579-5592</subfield></datafield></record></collection>
callnumber-first	Q - Science
author	Yanli Zhou
spellingShingle	Yanli Zhou misc QH301-705.5 misc calcineurin B-like protein misc cold tolerance misc <i<Stipa capillacea</i< misc transcriptome misc tonoplast misc Biology (General) Cold Tolerance of <i<ScCBL6</i< Is Associated with Tonoplast Transporters and Photosynthesis in <i<Arabidopsis</i<
authorStr	Yanli Zhou
ppnlink_with_tag_str_mv	@@773@@(DE-627)355690365
format	electronic Article
delete_txt_mv	keep
author_role	aut aut aut aut aut aut aut aut aut
collection	DOAJ
remote_str	true
callnumber-label	QH301-705
illustrated	Not Illustrated
issn	14673045
topic_title	QH301-705.5 Cold Tolerance of <i<ScCBL6</i< Is Associated with Tonoplast Transporters and Photosynthesis in <i<Arabidopsis</i< calcineurin B-like protein cold tolerance <i<Stipa capillacea</i< transcriptome tonoplast
topic	misc QH301-705.5 misc calcineurin B-like protein misc cold tolerance misc <i<Stipa capillacea</i< misc transcriptome misc tonoplast misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc calcineurin B-like protein misc cold tolerance misc <i<Stipa capillacea</i< misc transcriptome misc tonoplast misc Biology (General)
topic_browse	misc QH301-705.5 misc calcineurin B-like protein misc cold tolerance misc <i<Stipa capillacea</i< misc transcriptome misc tonoplast misc Biology (General)
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
format_main_str_mv	Text Zeitschrift/Artikel
carriertype_str_mv	cr
hierarchy_parent_title	Current Issues in Molecular Biology
hierarchy_parent_id	355690365
hierarchy_top_title	Current Issues in Molecular Biology
isfreeaccess_txt	true
familylinks_str_mv	(DE-627)355690365 (DE-600)2090836-2
title	Cold Tolerance of <i<ScCBL6</i< Is Associated with Tonoplast Transporters and Photosynthesis in <i<Arabidopsis</i<
ctrlnum	(DE-627)DOAJ083494065 (DE-599)DOAJ87ee7bb0ed2f485aaa52ddb8ec074b65
title_full	Cold Tolerance of <i<ScCBL6</i< Is Associated with Tonoplast Transporters and Photosynthesis in <i<Arabidopsis</i<
author_sort	Yanli Zhou
journal	Current Issues in Molecular Biology
journalStr	Current Issues in Molecular Biology
callnumber-first-code	Q
lang_code	eng
isOA_bool	true
recordtype	marc
publishDateSort	2022
contenttype_str_mv	txt
container_start_page	5579
author_browse	Yanli Zhou Jingling Zhang Changhong Zhao Guangqiang Long Chengli Zhou Xudong Sun Yunqiang Yang Chengjun Zhang Yongping Yang
container_volume	44
class	QH301-705.5
format_se	Elektronische Aufsätze
author-letter	Yanli Zhou
doi_str_mv	10.3390/cimb44110378
author2-role	verfasserin
title_sort	cold tolerance of <i<sccbl6</i< is associated with tonoplast transporters and photosynthesis in <i<arabidopsis</i<
callnumber	QH301-705.5
title_auth	Cold Tolerance of <i<ScCBL6</i< Is Associated with Tonoplast Transporters and Photosynthesis in <i<Arabidopsis</i<
abstract	Plants that are adapted to harsh environments offer enormous opportunity to understand stress responses in ecological systems. <i<Stipa capillacea</i< is widely distributed in the frigid and arid region of the Tibetan Plateau, but its signal transduction system under cold stress has not been characterized. In this study, we isolated a cDNA encoding the signal transduction protein, ScCBL6, from <i<S</i<. <i<capillacea</i<, and evaluated its role in cold tolerance by ectopically expressing it in <i<Arabidopsis</i<. Full-length <i<ScCBL6</i< encode 227 amino acids, and are clustered with CBL6 in <i<Stipa purpurea</i< and <i<Oryza sativa</i< in a phylogenetic analysis. Compared with tolerance in wild-type (WT) plants, ScCBL6-overexpressing plants (<i<ScCBL6-OXP</i<) were more tolerant to cold stress but not to drought stress, as confirmed by their high photosynthetic capacity (Fv/Fm) and survival rate under cold stress. We further compared their cold-responsive transcriptome profiles by RNA sequencing. In total, 3931 genes were differentially expressed by the introduction of <i<ScCBL6</i<. These gene products were involved in multiple processes such as the immune system, lipid catabolism, and secondary metabolism. A KEGG pathway analysis revealed that they were mainly enriched in plant hormone signal transduction and biomacromolecule metabolism. Proteins encoded by differentially expressed genes were predicted to be localized in chloroplasts, mitochondria, and vacuoles, suggesting that ScCBL6 exerts a wide range of functions. Based on its tonoplast subcellular location combined with integrated transcriptome and physiological analyses of <i<ScCBL6-OXP</i<, we inferred that ScCBL6 improves plant cold stress tolerance in <i<Arabidopsis</i< via the regulation of photosynthesis, redox status, and tonoplast metabolite transporters.
abstractGer	Plants that are adapted to harsh environments offer enormous opportunity to understand stress responses in ecological systems. <i<Stipa capillacea</i< is widely distributed in the frigid and arid region of the Tibetan Plateau, but its signal transduction system under cold stress has not been characterized. In this study, we isolated a cDNA encoding the signal transduction protein, ScCBL6, from <i<S</i<. <i<capillacea</i<, and evaluated its role in cold tolerance by ectopically expressing it in <i<Arabidopsis</i<. Full-length <i<ScCBL6</i< encode 227 amino acids, and are clustered with CBL6 in <i<Stipa purpurea</i< and <i<Oryza sativa</i< in a phylogenetic analysis. Compared with tolerance in wild-type (WT) plants, ScCBL6-overexpressing plants (<i<ScCBL6-OXP</i<) were more tolerant to cold stress but not to drought stress, as confirmed by their high photosynthetic capacity (Fv/Fm) and survival rate under cold stress. We further compared their cold-responsive transcriptome profiles by RNA sequencing. In total, 3931 genes were differentially expressed by the introduction of <i<ScCBL6</i<. These gene products were involved in multiple processes such as the immune system, lipid catabolism, and secondary metabolism. A KEGG pathway analysis revealed that they were mainly enriched in plant hormone signal transduction and biomacromolecule metabolism. Proteins encoded by differentially expressed genes were predicted to be localized in chloroplasts, mitochondria, and vacuoles, suggesting that ScCBL6 exerts a wide range of functions. Based on its tonoplast subcellular location combined with integrated transcriptome and physiological analyses of <i<ScCBL6-OXP</i<, we inferred that ScCBL6 improves plant cold stress tolerance in <i<Arabidopsis</i< via the regulation of photosynthesis, redox status, and tonoplast metabolite transporters.
abstract_unstemmed	Plants that are adapted to harsh environments offer enormous opportunity to understand stress responses in ecological systems. <i<Stipa capillacea</i< is widely distributed in the frigid and arid region of the Tibetan Plateau, but its signal transduction system under cold stress has not been characterized. In this study, we isolated a cDNA encoding the signal transduction protein, ScCBL6, from <i<S</i<. <i<capillacea</i<, and evaluated its role in cold tolerance by ectopically expressing it in <i<Arabidopsis</i<. Full-length <i<ScCBL6</i< encode 227 amino acids, and are clustered with CBL6 in <i<Stipa purpurea</i< and <i<Oryza sativa</i< in a phylogenetic analysis. Compared with tolerance in wild-type (WT) plants, ScCBL6-overexpressing plants (<i<ScCBL6-OXP</i<) were more tolerant to cold stress but not to drought stress, as confirmed by their high photosynthetic capacity (Fv/Fm) and survival rate under cold stress. We further compared their cold-responsive transcriptome profiles by RNA sequencing. In total, 3931 genes were differentially expressed by the introduction of <i<ScCBL6</i<. These gene products were involved in multiple processes such as the immune system, lipid catabolism, and secondary metabolism. A KEGG pathway analysis revealed that they were mainly enriched in plant hormone signal transduction and biomacromolecule metabolism. Proteins encoded by differentially expressed genes were predicted to be localized in chloroplasts, mitochondria, and vacuoles, suggesting that ScCBL6 exerts a wide range of functions. Based on its tonoplast subcellular location combined with integrated transcriptome and physiological analyses of <i<ScCBL6-OXP</i<, we inferred that ScCBL6 improves plant cold stress tolerance in <i<Arabidopsis</i< via the regulation of photosynthesis, redox status, and tonoplast metabolite transporters.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
container_issue	11
title_short	Cold Tolerance of <i<ScCBL6</i< Is Associated with Tonoplast Transporters and Photosynthesis in <i<Arabidopsis</i<
url	https://doi.org/10.3390/cimb44110378 https://doaj.org/article/87ee7bb0ed2f485aaa52ddb8ec074b65 https://www.mdpi.com/1467-3045/44/11/378 https://doaj.org/toc/1467-3037 https://doaj.org/toc/1467-3045
remote_bool	true
author2	Jingling Zhang Changhong Zhao Guangqiang Long Chengli Zhou Xudong Sun Yunqiang Yang Chengjun Zhang Yongping Yang
author2Str	Jingling Zhang Changhong Zhao Guangqiang Long Chengli Zhou Xudong Sun Yunqiang Yang Chengjun Zhang Yongping Yang
ppnlink	355690365
callnumber-subject	QH - Natural History and Biology
mediatype_str_mv	c
isOA_txt	true
hochschulschrift_bool	false
doi_str	10.3390/cimb44110378
callnumber-a	QH301-705.5
up_date	2024-07-03T17:47:02.233Z
_version_	1803580942304935936
fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ083494065</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240414172756.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230311s2022 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3390/cimb44110378</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ083494065</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ87ee7bb0ed2f485aaa52ddb8ec074b65</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH301-705.5</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Yanli Zhou</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Cold Tolerance of <i<ScCBL6</i< Is Associated with Tonoplast Transporters and Photosynthesis in <i<Arabidopsis</i<</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2022</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Plants that are adapted to harsh environments offer enormous opportunity to understand stress responses in ecological systems. <i<Stipa capillacea</i< is widely distributed in the frigid and arid region of the Tibetan Plateau, but its signal transduction system under cold stress has not been characterized. In this study, we isolated a cDNA encoding the signal transduction protein, ScCBL6, from <i<S</i<. <i<capillacea</i<, and evaluated its role in cold tolerance by ectopically expressing it in <i<Arabidopsis</i<. Full-length <i<ScCBL6</i< encode 227 amino acids, and are clustered with CBL6 in <i<Stipa purpurea</i< and <i<Oryza sativa</i< in a phylogenetic analysis. Compared with tolerance in wild-type (WT) plants, ScCBL6-overexpressing plants (<i<ScCBL6-OXP</i<) were more tolerant to cold stress but not to drought stress, as confirmed by their high photosynthetic capacity (Fv/Fm) and survival rate under cold stress. We further compared their cold-responsive transcriptome profiles by RNA sequencing. In total, 3931 genes were differentially expressed by the introduction of <i<ScCBL6</i<. These gene products were involved in multiple processes such as the immune system, lipid catabolism, and secondary metabolism. A KEGG pathway analysis revealed that they were mainly enriched in plant hormone signal transduction and biomacromolecule metabolism. Proteins encoded by differentially expressed genes were predicted to be localized in chloroplasts, mitochondria, and vacuoles, suggesting that ScCBL6 exerts a wide range of functions. Based on its tonoplast subcellular location combined with integrated transcriptome and physiological analyses of <i<ScCBL6-OXP</i<, we inferred that ScCBL6 improves plant cold stress tolerance in <i<Arabidopsis</i< via the regulation of photosynthesis, redox status, and tonoplast metabolite transporters.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">calcineurin B-like protein</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">cold tolerance</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><i<Stipa capillacea</i<</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">transcriptome</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">tonoplast</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Biology (General)</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Jingling Zhang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Changhong Zhao</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Guangqiang Long</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Chengli Zhou</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Xudong Sun</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Yunqiang Yang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Chengjun Zhang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Yongping Yang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Current Issues in Molecular Biology</subfield><subfield code="d">MDPI AG, 2021</subfield><subfield code="g">44(2022), 11, Seite 5579-5592</subfield><subfield code="w">(DE-627)355690365</subfield><subfield code="w">(DE-600)2090836-2</subfield><subfield code="x">14673045</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:44</subfield><subfield code="g">year:2022</subfield><subfield code="g">number:11</subfield><subfield code="g">pages:5579-5592</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.3390/cimb44110378</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/87ee7bb0ed2f485aaa52ddb8ec074b65</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.mdpi.com/1467-3045/44/11/378</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1467-3037</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1467-3045</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">44</subfield><subfield code="j">2022</subfield><subfield code="e">11</subfield><subfield code="h">5579-5592</subfield></datafield></record></collection>
score	7.399663

Nicht das Richtige dabei?

Schreiben Sie uns!

Cold Tolerance of <i<ScCBL6</i< Is Associated with Tonoplast Transporters and Photosynthesis in <i<Arabidopsis</i<

Nicht das Richtige dabei?

Zugang & Verfügbarkeit

Vorhandene Bände

Nicht das Richtige dabei?