Functional Evaluation of 3D Liver Models Labeled with Polysaccharide Functionalized Magnetic Nanoparticles

Establishing a rapid <i<in vitro</i< evaluation system for drug screening is essential for the development of new drugs. To reproduce tissues/organs with functions closer to living organisms, <i<in vitro</i< three-dimensional (3D) culture evaluation using microfabrication tec...
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Autor*in:	Yoshitaka Miyamoto [verfasserIn] Yumie Koshidaka [verfasserIn] Katsutoshi Murase [verfasserIn] Shoichiro Kanno [verfasserIn] Hirofumi Noguchi [verfasserIn] Kenji Miyado [verfasserIn] Takeshi Ikeya [verfasserIn] Satoshi Suzuki [verfasserIn] Tohru Yagi [verfasserIn] Naozumi Teramoto [verfasserIn] Shuji Hayashi [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	contrast agents magnetic iron oxide nanoparticles cell labeling 3D culture primary hepatocytes endothelial cell

Übergeordnetes Werk:	In: Materials - MDPI AG, 2009, 15(2022), 21, p 7823
Übergeordnetes Werk:	volume:15 ; year:2022 ; number:21, p 7823

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/ma15217823

Katalog-ID:	DOAJ083580484

Internformat


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allfields	10.3390/ma15217823 doi (DE-627)DOAJ083580484 (DE-599)DOAJc38b4540801e4e50a5420efc06b25a24 DE-627 ger DE-627 rakwb eng TK1-9971 TA1-2040 QH201-278.5 QC120-168.85 Yoshitaka Miyamoto verfasserin aut Functional Evaluation of 3D Liver Models Labeled with Polysaccharide Functionalized Magnetic Nanoparticles 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Establishing a rapid <i<in vitro</i< evaluation system for drug screening is essential for the development of new drugs. To reproduce tissues/organs with functions closer to living organisms, <i<in vitro</i< three-dimensional (3D) culture evaluation using microfabrication technology has been reported in recent years. Culture on patterned substrates with controlled hydrophilic and hydrophobic regions (Cell-able<sup<TM</sup<) can create 3D liver models (miniature livers) with liver-specific Disse luminal structures and functions. MRI contrast agents are widely used as safe and minimally invasive diagnostic methods. We focused on anionic polysaccharide magnetic iron oxide nanoparticles (Resovist<sup<®</sup<) and synthesized the four types of nanoparticle derivatives with different properties. Cationic nanoparticles (TMADM) can be used to label target cells in a short time and have been successfully visualized <i<in vivo</i<. In this study, we examined the morphology of various nanoparticles. The morphology of various nanoparticles showed relatively smooth-edged spherical shapes. As 3D liver models, we prepared primary hepatocyte–endothelial cell heterospheroids. The toxicity, CYP3A, and albumin secretory capacity were evaluated in the heterospheroids labeled with various nanoparticles. As the culture period progressed, the heterospheroids labeled with anionic and cationic nanoparticles showed lower liver function than non-labeled heterospheroids. In the future, there is a need to improve the method of creation of artificial 3D liver or to design a low-invasive MRI contrast agent to label the artificial 3D liver. contrast agents magnetic iron oxide nanoparticles cell labeling 3D culture primary hepatocytes endothelial cell Technology T Electrical engineering. Electronics. Nuclear engineering Engineering (General). Civil engineering (General) Microscopy Descriptive and experimental mechanics Yumie Koshidaka verfasserin aut Katsutoshi Murase verfasserin aut Shoichiro Kanno verfasserin aut Hirofumi Noguchi verfasserin aut Kenji Miyado verfasserin aut Takeshi Ikeya verfasserin aut Satoshi Suzuki verfasserin aut Tohru Yagi verfasserin aut Naozumi Teramoto verfasserin aut Shuji Hayashi verfasserin aut In Materials MDPI AG, 2009 15(2022), 21, p 7823 (DE-627)595712649 (DE-600)2487261-1 19961944 nnns volume:15 year:2022 number:21, p 7823 https://doi.org/10.3390/ma15217823 kostenfrei https://doaj.org/article/c38b4540801e4e50a5420efc06b25a24 kostenfrei https://www.mdpi.com/1996-1944/15/21/7823 kostenfrei https://doaj.org/toc/1996-1944 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 21, p 7823
spelling	10.3390/ma15217823 doi (DE-627)DOAJ083580484 (DE-599)DOAJc38b4540801e4e50a5420efc06b25a24 DE-627 ger DE-627 rakwb eng TK1-9971 TA1-2040 QH201-278.5 QC120-168.85 Yoshitaka Miyamoto verfasserin aut Functional Evaluation of 3D Liver Models Labeled with Polysaccharide Functionalized Magnetic Nanoparticles 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Establishing a rapid <i<in vitro</i< evaluation system for drug screening is essential for the development of new drugs. To reproduce tissues/organs with functions closer to living organisms, <i<in vitro</i< three-dimensional (3D) culture evaluation using microfabrication technology has been reported in recent years. Culture on patterned substrates with controlled hydrophilic and hydrophobic regions (Cell-able<sup<TM</sup<) can create 3D liver models (miniature livers) with liver-specific Disse luminal structures and functions. MRI contrast agents are widely used as safe and minimally invasive diagnostic methods. We focused on anionic polysaccharide magnetic iron oxide nanoparticles (Resovist<sup<®</sup<) and synthesized the four types of nanoparticle derivatives with different properties. Cationic nanoparticles (TMADM) can be used to label target cells in a short time and have been successfully visualized <i<in vivo</i<. In this study, we examined the morphology of various nanoparticles. The morphology of various nanoparticles showed relatively smooth-edged spherical shapes. As 3D liver models, we prepared primary hepatocyte–endothelial cell heterospheroids. The toxicity, CYP3A, and albumin secretory capacity were evaluated in the heterospheroids labeled with various nanoparticles. As the culture period progressed, the heterospheroids labeled with anionic and cationic nanoparticles showed lower liver function than non-labeled heterospheroids. In the future, there is a need to improve the method of creation of artificial 3D liver or to design a low-invasive MRI contrast agent to label the artificial 3D liver. contrast agents magnetic iron oxide nanoparticles cell labeling 3D culture primary hepatocytes endothelial cell Technology T Electrical engineering. Electronics. Nuclear engineering Engineering (General). Civil engineering (General) Microscopy Descriptive and experimental mechanics Yumie Koshidaka verfasserin aut Katsutoshi Murase verfasserin aut Shoichiro Kanno verfasserin aut Hirofumi Noguchi verfasserin aut Kenji Miyado verfasserin aut Takeshi Ikeya verfasserin aut Satoshi Suzuki verfasserin aut Tohru Yagi verfasserin aut Naozumi Teramoto verfasserin aut Shuji Hayashi verfasserin aut In Materials MDPI AG, 2009 15(2022), 21, p 7823 (DE-627)595712649 (DE-600)2487261-1 19961944 nnns volume:15 year:2022 number:21, p 7823 https://doi.org/10.3390/ma15217823 kostenfrei https://doaj.org/article/c38b4540801e4e50a5420efc06b25a24 kostenfrei https://www.mdpi.com/1996-1944/15/21/7823 kostenfrei https://doaj.org/toc/1996-1944 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 21, p 7823
allfields_unstemmed	10.3390/ma15217823 doi (DE-627)DOAJ083580484 (DE-599)DOAJc38b4540801e4e50a5420efc06b25a24 DE-627 ger DE-627 rakwb eng TK1-9971 TA1-2040 QH201-278.5 QC120-168.85 Yoshitaka Miyamoto verfasserin aut Functional Evaluation of 3D Liver Models Labeled with Polysaccharide Functionalized Magnetic Nanoparticles 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Establishing a rapid <i<in vitro</i< evaluation system for drug screening is essential for the development of new drugs. To reproduce tissues/organs with functions closer to living organisms, <i<in vitro</i< three-dimensional (3D) culture evaluation using microfabrication technology has been reported in recent years. Culture on patterned substrates with controlled hydrophilic and hydrophobic regions (Cell-able<sup<TM</sup<) can create 3D liver models (miniature livers) with liver-specific Disse luminal structures and functions. MRI contrast agents are widely used as safe and minimally invasive diagnostic methods. We focused on anionic polysaccharide magnetic iron oxide nanoparticles (Resovist<sup<®</sup<) and synthesized the four types of nanoparticle derivatives with different properties. Cationic nanoparticles (TMADM) can be used to label target cells in a short time and have been successfully visualized <i<in vivo</i<. In this study, we examined the morphology of various nanoparticles. The morphology of various nanoparticles showed relatively smooth-edged spherical shapes. As 3D liver models, we prepared primary hepatocyte–endothelial cell heterospheroids. The toxicity, CYP3A, and albumin secretory capacity were evaluated in the heterospheroids labeled with various nanoparticles. As the culture period progressed, the heterospheroids labeled with anionic and cationic nanoparticles showed lower liver function than non-labeled heterospheroids. In the future, there is a need to improve the method of creation of artificial 3D liver or to design a low-invasive MRI contrast agent to label the artificial 3D liver. contrast agents magnetic iron oxide nanoparticles cell labeling 3D culture primary hepatocytes endothelial cell Technology T Electrical engineering. Electronics. Nuclear engineering Engineering (General). Civil engineering (General) Microscopy Descriptive and experimental mechanics Yumie Koshidaka verfasserin aut Katsutoshi Murase verfasserin aut Shoichiro Kanno verfasserin aut Hirofumi Noguchi verfasserin aut Kenji Miyado verfasserin aut Takeshi Ikeya verfasserin aut Satoshi Suzuki verfasserin aut Tohru Yagi verfasserin aut Naozumi Teramoto verfasserin aut Shuji Hayashi verfasserin aut In Materials MDPI AG, 2009 15(2022), 21, p 7823 (DE-627)595712649 (DE-600)2487261-1 19961944 nnns volume:15 year:2022 number:21, p 7823 https://doi.org/10.3390/ma15217823 kostenfrei https://doaj.org/article/c38b4540801e4e50a5420efc06b25a24 kostenfrei https://www.mdpi.com/1996-1944/15/21/7823 kostenfrei https://doaj.org/toc/1996-1944 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 21, p 7823
allfieldsGer	10.3390/ma15217823 doi (DE-627)DOAJ083580484 (DE-599)DOAJc38b4540801e4e50a5420efc06b25a24 DE-627 ger DE-627 rakwb eng TK1-9971 TA1-2040 QH201-278.5 QC120-168.85 Yoshitaka Miyamoto verfasserin aut Functional Evaluation of 3D Liver Models Labeled with Polysaccharide Functionalized Magnetic Nanoparticles 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Establishing a rapid <i<in vitro</i< evaluation system for drug screening is essential for the development of new drugs. To reproduce tissues/organs with functions closer to living organisms, <i<in vitro</i< three-dimensional (3D) culture evaluation using microfabrication technology has been reported in recent years. Culture on patterned substrates with controlled hydrophilic and hydrophobic regions (Cell-able<sup<TM</sup<) can create 3D liver models (miniature livers) with liver-specific Disse luminal structures and functions. MRI contrast agents are widely used as safe and minimally invasive diagnostic methods. We focused on anionic polysaccharide magnetic iron oxide nanoparticles (Resovist<sup<®</sup<) and synthesized the four types of nanoparticle derivatives with different properties. Cationic nanoparticles (TMADM) can be used to label target cells in a short time and have been successfully visualized <i<in vivo</i<. In this study, we examined the morphology of various nanoparticles. The morphology of various nanoparticles showed relatively smooth-edged spherical shapes. As 3D liver models, we prepared primary hepatocyte–endothelial cell heterospheroids. The toxicity, CYP3A, and albumin secretory capacity were evaluated in the heterospheroids labeled with various nanoparticles. As the culture period progressed, the heterospheroids labeled with anionic and cationic nanoparticles showed lower liver function than non-labeled heterospheroids. In the future, there is a need to improve the method of creation of artificial 3D liver or to design a low-invasive MRI contrast agent to label the artificial 3D liver. contrast agents magnetic iron oxide nanoparticles cell labeling 3D culture primary hepatocytes endothelial cell Technology T Electrical engineering. Electronics. Nuclear engineering Engineering (General). Civil engineering (General) Microscopy Descriptive and experimental mechanics Yumie Koshidaka verfasserin aut Katsutoshi Murase verfasserin aut Shoichiro Kanno verfasserin aut Hirofumi Noguchi verfasserin aut Kenji Miyado verfasserin aut Takeshi Ikeya verfasserin aut Satoshi Suzuki verfasserin aut Tohru Yagi verfasserin aut Naozumi Teramoto verfasserin aut Shuji Hayashi verfasserin aut In Materials MDPI AG, 2009 15(2022), 21, p 7823 (DE-627)595712649 (DE-600)2487261-1 19961944 nnns volume:15 year:2022 number:21, p 7823 https://doi.org/10.3390/ma15217823 kostenfrei https://doaj.org/article/c38b4540801e4e50a5420efc06b25a24 kostenfrei https://www.mdpi.com/1996-1944/15/21/7823 kostenfrei https://doaj.org/toc/1996-1944 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 21, p 7823
allfieldsSound	10.3390/ma15217823 doi (DE-627)DOAJ083580484 (DE-599)DOAJc38b4540801e4e50a5420efc06b25a24 DE-627 ger DE-627 rakwb eng TK1-9971 TA1-2040 QH201-278.5 QC120-168.85 Yoshitaka Miyamoto verfasserin aut Functional Evaluation of 3D Liver Models Labeled with Polysaccharide Functionalized Magnetic Nanoparticles 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Establishing a rapid <i<in vitro</i< evaluation system for drug screening is essential for the development of new drugs. To reproduce tissues/organs with functions closer to living organisms, <i<in vitro</i< three-dimensional (3D) culture evaluation using microfabrication technology has been reported in recent years. Culture on patterned substrates with controlled hydrophilic and hydrophobic regions (Cell-able<sup<TM</sup<) can create 3D liver models (miniature livers) with liver-specific Disse luminal structures and functions. MRI contrast agents are widely used as safe and minimally invasive diagnostic methods. We focused on anionic polysaccharide magnetic iron oxide nanoparticles (Resovist<sup<®</sup<) and synthesized the four types of nanoparticle derivatives with different properties. Cationic nanoparticles (TMADM) can be used to label target cells in a short time and have been successfully visualized <i<in vivo</i<. In this study, we examined the morphology of various nanoparticles. The morphology of various nanoparticles showed relatively smooth-edged spherical shapes. As 3D liver models, we prepared primary hepatocyte–endothelial cell heterospheroids. The toxicity, CYP3A, and albumin secretory capacity were evaluated in the heterospheroids labeled with various nanoparticles. As the culture period progressed, the heterospheroids labeled with anionic and cationic nanoparticles showed lower liver function than non-labeled heterospheroids. In the future, there is a need to improve the method of creation of artificial 3D liver or to design a low-invasive MRI contrast agent to label the artificial 3D liver. contrast agents magnetic iron oxide nanoparticles cell labeling 3D culture primary hepatocytes endothelial cell Technology T Electrical engineering. Electronics. Nuclear engineering Engineering (General). Civil engineering (General) Microscopy Descriptive and experimental mechanics Yumie Koshidaka verfasserin aut Katsutoshi Murase verfasserin aut Shoichiro Kanno verfasserin aut Hirofumi Noguchi verfasserin aut Kenji Miyado verfasserin aut Takeshi Ikeya verfasserin aut Satoshi Suzuki verfasserin aut Tohru Yagi verfasserin aut Naozumi Teramoto verfasserin aut Shuji Hayashi verfasserin aut In Materials MDPI AG, 2009 15(2022), 21, p 7823 (DE-627)595712649 (DE-600)2487261-1 19961944 nnns volume:15 year:2022 number:21, p 7823 https://doi.org/10.3390/ma15217823 kostenfrei https://doaj.org/article/c38b4540801e4e50a5420efc06b25a24 kostenfrei https://www.mdpi.com/1996-1944/15/21/7823 kostenfrei https://doaj.org/toc/1996-1944 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 21, p 7823
language	English
source	In Materials 15(2022), 21, p 7823 volume:15 year:2022 number:21, p 7823
sourceStr	In Materials 15(2022), 21, p 7823 volume:15 year:2022 number:21, p 7823
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	contrast agents magnetic iron oxide nanoparticles cell labeling 3D culture primary hepatocytes endothelial cell Technology T Electrical engineering. Electronics. Nuclear engineering Engineering (General). Civil engineering (General) Microscopy Descriptive and experimental mechanics
isfreeaccess_bool	true
container_title	Materials
authorswithroles_txt_mv	Yoshitaka Miyamoto @@aut@@ Yumie Koshidaka @@aut@@ Katsutoshi Murase @@aut@@ Shoichiro Kanno @@aut@@ Hirofumi Noguchi @@aut@@ Kenji Miyado @@aut@@ Takeshi Ikeya @@aut@@ Satoshi Suzuki @@aut@@ Tohru Yagi @@aut@@ Naozumi Teramoto @@aut@@ Shuji Hayashi @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	595712649
id	DOAJ083580484
language_de	englisch
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callnumber-first	T - Technology
author	Yoshitaka Miyamoto
spellingShingle	Yoshitaka Miyamoto misc TK1-9971 misc TA1-2040 misc QH201-278.5 misc QC120-168.85 misc contrast agents misc magnetic iron oxide nanoparticles misc cell labeling misc 3D culture misc primary hepatocytes misc endothelial cell misc Technology misc T misc Electrical engineering. Electronics. Nuclear engineering misc Engineering (General). Civil engineering (General) misc Microscopy misc Descriptive and experimental mechanics Functional Evaluation of 3D Liver Models Labeled with Polysaccharide Functionalized Magnetic Nanoparticles
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topic_title	TK1-9971 TA1-2040 QH201-278.5 QC120-168.85 Functional Evaluation of 3D Liver Models Labeled with Polysaccharide Functionalized Magnetic Nanoparticles contrast agents magnetic iron oxide nanoparticles cell labeling 3D culture primary hepatocytes endothelial cell
topic	misc TK1-9971 misc TA1-2040 misc QH201-278.5 misc QC120-168.85 misc contrast agents misc magnetic iron oxide nanoparticles misc cell labeling misc 3D culture misc primary hepatocytes misc endothelial cell misc Technology misc T misc Electrical engineering. Electronics. Nuclear engineering misc Engineering (General). Civil engineering (General) misc Microscopy misc Descriptive and experimental mechanics
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title	Functional Evaluation of 3D Liver Models Labeled with Polysaccharide Functionalized Magnetic Nanoparticles
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title_full	Functional Evaluation of 3D Liver Models Labeled with Polysaccharide Functionalized Magnetic Nanoparticles
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author_browse	Yoshitaka Miyamoto Yumie Koshidaka Katsutoshi Murase Shoichiro Kanno Hirofumi Noguchi Kenji Miyado Takeshi Ikeya Satoshi Suzuki Tohru Yagi Naozumi Teramoto Shuji Hayashi
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title_sort	functional evaluation of 3d liver models labeled with polysaccharide functionalized magnetic nanoparticles
callnumber	TK1-9971
title_auth	Functional Evaluation of 3D Liver Models Labeled with Polysaccharide Functionalized Magnetic Nanoparticles
abstract	Establishing a rapid <i<in vitro</i< evaluation system for drug screening is essential for the development of new drugs. To reproduce tissues/organs with functions closer to living organisms, <i<in vitro</i< three-dimensional (3D) culture evaluation using microfabrication technology has been reported in recent years. Culture on patterned substrates with controlled hydrophilic and hydrophobic regions (Cell-able<sup<TM</sup<) can create 3D liver models (miniature livers) with liver-specific Disse luminal structures and functions. MRI contrast agents are widely used as safe and minimally invasive diagnostic methods. We focused on anionic polysaccharide magnetic iron oxide nanoparticles (Resovist<sup<®</sup<) and synthesized the four types of nanoparticle derivatives with different properties. Cationic nanoparticles (TMADM) can be used to label target cells in a short time and have been successfully visualized <i<in vivo</i<. In this study, we examined the morphology of various nanoparticles. The morphology of various nanoparticles showed relatively smooth-edged spherical shapes. As 3D liver models, we prepared primary hepatocyte–endothelial cell heterospheroids. The toxicity, CYP3A, and albumin secretory capacity were evaluated in the heterospheroids labeled with various nanoparticles. As the culture period progressed, the heterospheroids labeled with anionic and cationic nanoparticles showed lower liver function than non-labeled heterospheroids. In the future, there is a need to improve the method of creation of artificial 3D liver or to design a low-invasive MRI contrast agent to label the artificial 3D liver.
abstractGer	Establishing a rapid <i<in vitro</i< evaluation system for drug screening is essential for the development of new drugs. To reproduce tissues/organs with functions closer to living organisms, <i<in vitro</i< three-dimensional (3D) culture evaluation using microfabrication technology has been reported in recent years. Culture on patterned substrates with controlled hydrophilic and hydrophobic regions (Cell-able<sup<TM</sup<) can create 3D liver models (miniature livers) with liver-specific Disse luminal structures and functions. MRI contrast agents are widely used as safe and minimally invasive diagnostic methods. We focused on anionic polysaccharide magnetic iron oxide nanoparticles (Resovist<sup<®</sup<) and synthesized the four types of nanoparticle derivatives with different properties. Cationic nanoparticles (TMADM) can be used to label target cells in a short time and have been successfully visualized <i<in vivo</i<. In this study, we examined the morphology of various nanoparticles. The morphology of various nanoparticles showed relatively smooth-edged spherical shapes. As 3D liver models, we prepared primary hepatocyte–endothelial cell heterospheroids. The toxicity, CYP3A, and albumin secretory capacity were evaluated in the heterospheroids labeled with various nanoparticles. As the culture period progressed, the heterospheroids labeled with anionic and cationic nanoparticles showed lower liver function than non-labeled heterospheroids. In the future, there is a need to improve the method of creation of artificial 3D liver or to design a low-invasive MRI contrast agent to label the artificial 3D liver.
abstract_unstemmed	Establishing a rapid <i<in vitro</i< evaluation system for drug screening is essential for the development of new drugs. To reproduce tissues/organs with functions closer to living organisms, <i<in vitro</i< three-dimensional (3D) culture evaluation using microfabrication technology has been reported in recent years. Culture on patterned substrates with controlled hydrophilic and hydrophobic regions (Cell-able<sup<TM</sup<) can create 3D liver models (miniature livers) with liver-specific Disse luminal structures and functions. MRI contrast agents are widely used as safe and minimally invasive diagnostic methods. We focused on anionic polysaccharide magnetic iron oxide nanoparticles (Resovist<sup<®</sup<) and synthesized the four types of nanoparticle derivatives with different properties. Cationic nanoparticles (TMADM) can be used to label target cells in a short time and have been successfully visualized <i<in vivo</i<. In this study, we examined the morphology of various nanoparticles. The morphology of various nanoparticles showed relatively smooth-edged spherical shapes. As 3D liver models, we prepared primary hepatocyte–endothelial cell heterospheroids. The toxicity, CYP3A, and albumin secretory capacity were evaluated in the heterospheroids labeled with various nanoparticles. As the culture period progressed, the heterospheroids labeled with anionic and cationic nanoparticles showed lower liver function than non-labeled heterospheroids. In the future, there is a need to improve the method of creation of artificial 3D liver or to design a low-invasive MRI contrast agent to label the artificial 3D liver.
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title_short	Functional Evaluation of 3D Liver Models Labeled with Polysaccharide Functionalized Magnetic Nanoparticles
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Functional Evaluation of 3D Liver Models Labeled with Polysaccharide Functionalized Magnetic Nanoparticles

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