Identification of Herpes Simplex Virus, Chlamydia Trachomatis, and Mycoplasma Genitalium in Infertile Seminal Fluid Samples Using Multiplex-PCR in Kerman Province, Iran (2016)

Introduction: Genitourinary tract infections are among the most common causes of infertility in men. These infections could also cause disorders in various parameters, such as the number, motility, viability, and morphology of sperms, as well as the function of the semen, epididymitis, and prostatit...
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Gespeichert in:

Autor*in:	Aida Pourmohamadi Siahkali [verfasserIn] Kumarss Amini [verfasserIn]

Format:	E-Artikel
Sprache:	Persisch

Erschienen:	2018

Schlagwörter:	seminal fluid microbial infection infertility

Übergeordnetes Werk:	In: Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān - Mashhad University of Medical Sciences, 2017, 21(2018), 2, Seite 69-74
Übergeordnetes Werk:	volume:21 ; year:2018 ; number:2 ; pages:69-74

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.22038/ijogi.2018.10714

Katalog-ID:	DOAJ083615067

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520			\|a Introduction: Genitourinary tract infections are among the most common causes of infertility in men. These infections could also cause disorders in various parameters, such as the number, motility, viability, and morphology of sperms, as well as the function of the semen, epididymitis, and prostatitis. The present study aimed to determine the prevalence of the bacterial and viral infections associated with the herpes virus, Chlamydia trachomatis, and Mycoplasma genitalium in the seminal fluid of infertile men with unknown cause. Methods: In this descriptive-analytical study, 60 seminal fluid samples were collected from the infertile men referring to an infertility treatment center in Kerman, Iran during May-November 2016. Multiplex-PCR was carried out using specific primers to identify the herpes virus, Chlamydia trachomatis, and Mycoplasma genitalium. Data analysis was performed in SPSS. Results: Out of 60 tested samples, eight cases (13.3%) were positive for the investigated organisms. Among the positive samples, three cases (37.5%) had HSV-1, three samples (37.5%) had C. trachomatis, one case (12.5%) had M. genitalium, and one sample (12.5%) had HSV-2. Conclusion: Findings of the present study regarding the presence of bacterial and viral organisms in the seminal fluid could lay the groundwork for the screening of asymptomatic infections and appropriate treatment before initiating assisted reproductive therapy.
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allfields	10.22038/ijogi.2018.10714 doi (DE-627)DOAJ083615067 (DE-599)DOAJ1ec03dac51e8499ab3ec4e27a55f820c DE-627 ger DE-627 rakwb per RG1-991 Aida Pourmohamadi Siahkali verfasserin aut Identification of Herpes Simplex Virus, Chlamydia Trachomatis, and Mycoplasma Genitalium in Infertile Seminal Fluid Samples Using Multiplex-PCR in Kerman Province, Iran (2016) 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Genitourinary tract infections are among the most common causes of infertility in men. These infections could also cause disorders in various parameters, such as the number, motility, viability, and morphology of sperms, as well as the function of the semen, epididymitis, and prostatitis. The present study aimed to determine the prevalence of the bacterial and viral infections associated with the herpes virus, Chlamydia trachomatis, and Mycoplasma genitalium in the seminal fluid of infertile men with unknown cause. Methods: In this descriptive-analytical study, 60 seminal fluid samples were collected from the infertile men referring to an infertility treatment center in Kerman, Iran during May-November 2016. Multiplex-PCR was carried out using specific primers to identify the herpes virus, Chlamydia trachomatis, and Mycoplasma genitalium. Data analysis was performed in SPSS. Results: Out of 60 tested samples, eight cases (13.3%) were positive for the investigated organisms. Among the positive samples, three cases (37.5%) had HSV-1, three samples (37.5%) had C. trachomatis, one case (12.5%) had M. genitalium, and one sample (12.5%) had HSV-2. Conclusion: Findings of the present study regarding the presence of bacterial and viral organisms in the seminal fluid could lay the groundwork for the screening of asymptomatic infections and appropriate treatment before initiating assisted reproductive therapy. seminal fluid microbial infection infertility Gynecology and obstetrics Kumarss Amini verfasserin aut In Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān Mashhad University of Medical Sciences, 2017 21(2018), 2, Seite 69-74 (DE-627)686947827 (DE-600)2651625-1 20082363 nnns volume:21 year:2018 number:2 pages:69-74 https://doi.org/10.22038/ijogi.2018.10714 kostenfrei https://doaj.org/article/1ec03dac51e8499ab3ec4e27a55f820c kostenfrei https://ijogi.mums.ac.ir/article_10714_dafcdeba5241bbf5464e940dbe85f666.pdf kostenfrei https://doaj.org/toc/1680-2993 Journal toc kostenfrei https://doaj.org/toc/2008-2363 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2018 2 69-74
spelling	10.22038/ijogi.2018.10714 doi (DE-627)DOAJ083615067 (DE-599)DOAJ1ec03dac51e8499ab3ec4e27a55f820c DE-627 ger DE-627 rakwb per RG1-991 Aida Pourmohamadi Siahkali verfasserin aut Identification of Herpes Simplex Virus, Chlamydia Trachomatis, and Mycoplasma Genitalium in Infertile Seminal Fluid Samples Using Multiplex-PCR in Kerman Province, Iran (2016) 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Genitourinary tract infections are among the most common causes of infertility in men. These infections could also cause disorders in various parameters, such as the number, motility, viability, and morphology of sperms, as well as the function of the semen, epididymitis, and prostatitis. The present study aimed to determine the prevalence of the bacterial and viral infections associated with the herpes virus, Chlamydia trachomatis, and Mycoplasma genitalium in the seminal fluid of infertile men with unknown cause. Methods: In this descriptive-analytical study, 60 seminal fluid samples were collected from the infertile men referring to an infertility treatment center in Kerman, Iran during May-November 2016. Multiplex-PCR was carried out using specific primers to identify the herpes virus, Chlamydia trachomatis, and Mycoplasma genitalium. Data analysis was performed in SPSS. Results: Out of 60 tested samples, eight cases (13.3%) were positive for the investigated organisms. Among the positive samples, three cases (37.5%) had HSV-1, three samples (37.5%) had C. trachomatis, one case (12.5%) had M. genitalium, and one sample (12.5%) had HSV-2. Conclusion: Findings of the present study regarding the presence of bacterial and viral organisms in the seminal fluid could lay the groundwork for the screening of asymptomatic infections and appropriate treatment before initiating assisted reproductive therapy. seminal fluid microbial infection infertility Gynecology and obstetrics Kumarss Amini verfasserin aut In Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān Mashhad University of Medical Sciences, 2017 21(2018), 2, Seite 69-74 (DE-627)686947827 (DE-600)2651625-1 20082363 nnns volume:21 year:2018 number:2 pages:69-74 https://doi.org/10.22038/ijogi.2018.10714 kostenfrei https://doaj.org/article/1ec03dac51e8499ab3ec4e27a55f820c kostenfrei https://ijogi.mums.ac.ir/article_10714_dafcdeba5241bbf5464e940dbe85f666.pdf kostenfrei https://doaj.org/toc/1680-2993 Journal toc kostenfrei https://doaj.org/toc/2008-2363 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2018 2 69-74
allfields_unstemmed	10.22038/ijogi.2018.10714 doi (DE-627)DOAJ083615067 (DE-599)DOAJ1ec03dac51e8499ab3ec4e27a55f820c DE-627 ger DE-627 rakwb per RG1-991 Aida Pourmohamadi Siahkali verfasserin aut Identification of Herpes Simplex Virus, Chlamydia Trachomatis, and Mycoplasma Genitalium in Infertile Seminal Fluid Samples Using Multiplex-PCR in Kerman Province, Iran (2016) 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Genitourinary tract infections are among the most common causes of infertility in men. These infections could also cause disorders in various parameters, such as the number, motility, viability, and morphology of sperms, as well as the function of the semen, epididymitis, and prostatitis. The present study aimed to determine the prevalence of the bacterial and viral infections associated with the herpes virus, Chlamydia trachomatis, and Mycoplasma genitalium in the seminal fluid of infertile men with unknown cause. Methods: In this descriptive-analytical study, 60 seminal fluid samples were collected from the infertile men referring to an infertility treatment center in Kerman, Iran during May-November 2016. Multiplex-PCR was carried out using specific primers to identify the herpes virus, Chlamydia trachomatis, and Mycoplasma genitalium. Data analysis was performed in SPSS. Results: Out of 60 tested samples, eight cases (13.3%) were positive for the investigated organisms. Among the positive samples, three cases (37.5%) had HSV-1, three samples (37.5%) had C. trachomatis, one case (12.5%) had M. genitalium, and one sample (12.5%) had HSV-2. Conclusion: Findings of the present study regarding the presence of bacterial and viral organisms in the seminal fluid could lay the groundwork for the screening of asymptomatic infections and appropriate treatment before initiating assisted reproductive therapy. seminal fluid microbial infection infertility Gynecology and obstetrics Kumarss Amini verfasserin aut In Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān Mashhad University of Medical Sciences, 2017 21(2018), 2, Seite 69-74 (DE-627)686947827 (DE-600)2651625-1 20082363 nnns volume:21 year:2018 number:2 pages:69-74 https://doi.org/10.22038/ijogi.2018.10714 kostenfrei https://doaj.org/article/1ec03dac51e8499ab3ec4e27a55f820c kostenfrei https://ijogi.mums.ac.ir/article_10714_dafcdeba5241bbf5464e940dbe85f666.pdf kostenfrei https://doaj.org/toc/1680-2993 Journal toc kostenfrei https://doaj.org/toc/2008-2363 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2018 2 69-74
allfieldsGer	10.22038/ijogi.2018.10714 doi (DE-627)DOAJ083615067 (DE-599)DOAJ1ec03dac51e8499ab3ec4e27a55f820c DE-627 ger DE-627 rakwb per RG1-991 Aida Pourmohamadi Siahkali verfasserin aut Identification of Herpes Simplex Virus, Chlamydia Trachomatis, and Mycoplasma Genitalium in Infertile Seminal Fluid Samples Using Multiplex-PCR in Kerman Province, Iran (2016) 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Genitourinary tract infections are among the most common causes of infertility in men. These infections could also cause disorders in various parameters, such as the number, motility, viability, and morphology of sperms, as well as the function of the semen, epididymitis, and prostatitis. The present study aimed to determine the prevalence of the bacterial and viral infections associated with the herpes virus, Chlamydia trachomatis, and Mycoplasma genitalium in the seminal fluid of infertile men with unknown cause. Methods: In this descriptive-analytical study, 60 seminal fluid samples were collected from the infertile men referring to an infertility treatment center in Kerman, Iran during May-November 2016. Multiplex-PCR was carried out using specific primers to identify the herpes virus, Chlamydia trachomatis, and Mycoplasma genitalium. Data analysis was performed in SPSS. Results: Out of 60 tested samples, eight cases (13.3%) were positive for the investigated organisms. Among the positive samples, three cases (37.5%) had HSV-1, three samples (37.5%) had C. trachomatis, one case (12.5%) had M. genitalium, and one sample (12.5%) had HSV-2. Conclusion: Findings of the present study regarding the presence of bacterial and viral organisms in the seminal fluid could lay the groundwork for the screening of asymptomatic infections and appropriate treatment before initiating assisted reproductive therapy. seminal fluid microbial infection infertility Gynecology and obstetrics Kumarss Amini verfasserin aut In Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān Mashhad University of Medical Sciences, 2017 21(2018), 2, Seite 69-74 (DE-627)686947827 (DE-600)2651625-1 20082363 nnns volume:21 year:2018 number:2 pages:69-74 https://doi.org/10.22038/ijogi.2018.10714 kostenfrei https://doaj.org/article/1ec03dac51e8499ab3ec4e27a55f820c kostenfrei https://ijogi.mums.ac.ir/article_10714_dafcdeba5241bbf5464e940dbe85f666.pdf kostenfrei https://doaj.org/toc/1680-2993 Journal toc kostenfrei https://doaj.org/toc/2008-2363 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2018 2 69-74
allfieldsSound	10.22038/ijogi.2018.10714 doi (DE-627)DOAJ083615067 (DE-599)DOAJ1ec03dac51e8499ab3ec4e27a55f820c DE-627 ger DE-627 rakwb per RG1-991 Aida Pourmohamadi Siahkali verfasserin aut Identification of Herpes Simplex Virus, Chlamydia Trachomatis, and Mycoplasma Genitalium in Infertile Seminal Fluid Samples Using Multiplex-PCR in Kerman Province, Iran (2016) 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Genitourinary tract infections are among the most common causes of infertility in men. These infections could also cause disorders in various parameters, such as the number, motility, viability, and morphology of sperms, as well as the function of the semen, epididymitis, and prostatitis. The present study aimed to determine the prevalence of the bacterial and viral infections associated with the herpes virus, Chlamydia trachomatis, and Mycoplasma genitalium in the seminal fluid of infertile men with unknown cause. Methods: In this descriptive-analytical study, 60 seminal fluid samples were collected from the infertile men referring to an infertility treatment center in Kerman, Iran during May-November 2016. Multiplex-PCR was carried out using specific primers to identify the herpes virus, Chlamydia trachomatis, and Mycoplasma genitalium. Data analysis was performed in SPSS. Results: Out of 60 tested samples, eight cases (13.3%) were positive for the investigated organisms. Among the positive samples, three cases (37.5%) had HSV-1, three samples (37.5%) had C. trachomatis, one case (12.5%) had M. genitalium, and one sample (12.5%) had HSV-2. Conclusion: Findings of the present study regarding the presence of bacterial and viral organisms in the seminal fluid could lay the groundwork for the screening of asymptomatic infections and appropriate treatment before initiating assisted reproductive therapy. seminal fluid microbial infection infertility Gynecology and obstetrics Kumarss Amini verfasserin aut In Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān Mashhad University of Medical Sciences, 2017 21(2018), 2, Seite 69-74 (DE-627)686947827 (DE-600)2651625-1 20082363 nnns volume:21 year:2018 number:2 pages:69-74 https://doi.org/10.22038/ijogi.2018.10714 kostenfrei https://doaj.org/article/1ec03dac51e8499ab3ec4e27a55f820c kostenfrei https://ijogi.mums.ac.ir/article_10714_dafcdeba5241bbf5464e940dbe85f666.pdf kostenfrei https://doaj.org/toc/1680-2993 Journal toc kostenfrei https://doaj.org/toc/2008-2363 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2018 2 69-74
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callnumber-first	R - Medicine
author	Aida Pourmohamadi Siahkali
spellingShingle	Aida Pourmohamadi Siahkali misc RG1-991 misc seminal fluid misc microbial infection misc infertility misc Gynecology and obstetrics Identification of Herpes Simplex Virus, Chlamydia Trachomatis, and Mycoplasma Genitalium in Infertile Seminal Fluid Samples Using Multiplex-PCR in Kerman Province, Iran (2016)
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topic_title	RG1-991 Identification of Herpes Simplex Virus, Chlamydia Trachomatis, and Mycoplasma Genitalium in Infertile Seminal Fluid Samples Using Multiplex-PCR in Kerman Province, Iran (2016) seminal fluid microbial infection infertility
topic	misc RG1-991 misc seminal fluid misc microbial infection misc infertility misc Gynecology and obstetrics
topic_unstemmed	misc RG1-991 misc seminal fluid misc microbial infection misc infertility misc Gynecology and obstetrics
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title	Identification of Herpes Simplex Virus, Chlamydia Trachomatis, and Mycoplasma Genitalium in Infertile Seminal Fluid Samples Using Multiplex-PCR in Kerman Province, Iran (2016)
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title_full	Identification of Herpes Simplex Virus, Chlamydia Trachomatis, and Mycoplasma Genitalium in Infertile Seminal Fluid Samples Using Multiplex-PCR in Kerman Province, Iran (2016)
author_sort	Aida Pourmohamadi Siahkali
journal	Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān
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title_sort	identification of herpes simplex virus, chlamydia trachomatis, and mycoplasma genitalium in infertile seminal fluid samples using multiplex-pcr in kerman province, iran (2016)
callnumber	RG1-991
title_auth	Identification of Herpes Simplex Virus, Chlamydia Trachomatis, and Mycoplasma Genitalium in Infertile Seminal Fluid Samples Using Multiplex-PCR in Kerman Province, Iran (2016)
abstract	Introduction: Genitourinary tract infections are among the most common causes of infertility in men. These infections could also cause disorders in various parameters, such as the number, motility, viability, and morphology of sperms, as well as the function of the semen, epididymitis, and prostatitis. The present study aimed to determine the prevalence of the bacterial and viral infections associated with the herpes virus, Chlamydia trachomatis, and Mycoplasma genitalium in the seminal fluid of infertile men with unknown cause. Methods: In this descriptive-analytical study, 60 seminal fluid samples were collected from the infertile men referring to an infertility treatment center in Kerman, Iran during May-November 2016. Multiplex-PCR was carried out using specific primers to identify the herpes virus, Chlamydia trachomatis, and Mycoplasma genitalium. Data analysis was performed in SPSS. Results: Out of 60 tested samples, eight cases (13.3%) were positive for the investigated organisms. Among the positive samples, three cases (37.5%) had HSV-1, three samples (37.5%) had C. trachomatis, one case (12.5%) had M. genitalium, and one sample (12.5%) had HSV-2. Conclusion: Findings of the present study regarding the presence of bacterial and viral organisms in the seminal fluid could lay the groundwork for the screening of asymptomatic infections and appropriate treatment before initiating assisted reproductive therapy.
abstractGer	Introduction: Genitourinary tract infections are among the most common causes of infertility in men. These infections could also cause disorders in various parameters, such as the number, motility, viability, and morphology of sperms, as well as the function of the semen, epididymitis, and prostatitis. The present study aimed to determine the prevalence of the bacterial and viral infections associated with the herpes virus, Chlamydia trachomatis, and Mycoplasma genitalium in the seminal fluid of infertile men with unknown cause. Methods: In this descriptive-analytical study, 60 seminal fluid samples were collected from the infertile men referring to an infertility treatment center in Kerman, Iran during May-November 2016. Multiplex-PCR was carried out using specific primers to identify the herpes virus, Chlamydia trachomatis, and Mycoplasma genitalium. Data analysis was performed in SPSS. Results: Out of 60 tested samples, eight cases (13.3%) were positive for the investigated organisms. Among the positive samples, three cases (37.5%) had HSV-1, three samples (37.5%) had C. trachomatis, one case (12.5%) had M. genitalium, and one sample (12.5%) had HSV-2. Conclusion: Findings of the present study regarding the presence of bacterial and viral organisms in the seminal fluid could lay the groundwork for the screening of asymptomatic infections and appropriate treatment before initiating assisted reproductive therapy.
abstract_unstemmed	Introduction: Genitourinary tract infections are among the most common causes of infertility in men. These infections could also cause disorders in various parameters, such as the number, motility, viability, and morphology of sperms, as well as the function of the semen, epididymitis, and prostatitis. The present study aimed to determine the prevalence of the bacterial and viral infections associated with the herpes virus, Chlamydia trachomatis, and Mycoplasma genitalium in the seminal fluid of infertile men with unknown cause. Methods: In this descriptive-analytical study, 60 seminal fluid samples were collected from the infertile men referring to an infertility treatment center in Kerman, Iran during May-November 2016. Multiplex-PCR was carried out using specific primers to identify the herpes virus, Chlamydia trachomatis, and Mycoplasma genitalium. Data analysis was performed in SPSS. Results: Out of 60 tested samples, eight cases (13.3%) were positive for the investigated organisms. Among the positive samples, three cases (37.5%) had HSV-1, three samples (37.5%) had C. trachomatis, one case (12.5%) had M. genitalium, and one sample (12.5%) had HSV-2. Conclusion: Findings of the present study regarding the presence of bacterial and viral organisms in the seminal fluid could lay the groundwork for the screening of asymptomatic infections and appropriate treatment before initiating assisted reproductive therapy.
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title_short	Identification of Herpes Simplex Virus, Chlamydia Trachomatis, and Mycoplasma Genitalium in Infertile Seminal Fluid Samples Using Multiplex-PCR in Kerman Province, Iran (2016)
url	https://doi.org/10.22038/ijogi.2018.10714 https://doaj.org/article/1ec03dac51e8499ab3ec4e27a55f820c https://ijogi.mums.ac.ir/article_10714_dafcdeba5241bbf5464e940dbe85f666.pdf https://doaj.org/toc/1680-2993 https://doaj.org/toc/2008-2363
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up_date	2024-07-03T18:27:30.765Z
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Among the positive samples, three cases (37.5%) had HSV-1, three samples (37.5%) had C. trachomatis, one case (12.5%) had M. genitalium, and one sample (12.5%) had HSV-2. 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Identification of Herpes Simplex Virus, Chlamydia Trachomatis, and Mycoplasma Genitalium in Infertile Seminal Fluid Samples Using Multiplex-PCR in Kerman Province, Iran (2016)

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