Single-cell transcriptome analysis reveals changes of T lymphocytes in peritoneal fluid from peritoneal dialysis patients
Objective To reveal the change trajectory of T lymphocytes in the dialysis effluent from peritoneal dialysis （PD） patients with the prolongation of PD time, and to explore its relationship with PD-associated peritoneal fibrosis. Methods Cell classification was performed on the single-cell transcript...
Ausführliche Beschreibung
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| Autor*in: |
Li Yongjie, Sun Yuxiang, Lin Hongchun, Li Canming, Shang Hongli, Peng Hui [verfasserIn] |
|---|
| Format: |
E-Artikel |
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| Sprache: |
Chinesisch |
| Erschienen: |
2022 |
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| Schlagwörter: |
|single-cell transcriptome analysis|peritoneal dialysis|peritoneal fibrosis|t lymphocyte |
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| Übergeordnetes Werk: |
In: Xin yixue - Editorial Office of Journal of New Medicine, 2022, 53(2022), 4, Seite 292-296 |
|---|---|
| Übergeordnetes Werk: |
volume:53 ; year:2022 ; number:4 ; pages:292-296 |
| Links: |
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| DOI / URN: |
10.3969/j.issn.0253-9802.2022.04.013 |
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DOAJ083780726 |
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| 520 | |a Objective To reveal the change trajectory of T lymphocytes in the dialysis effluent from peritoneal dialysis （PD） patients with the prolongation of PD time, and to explore its relationship with PD-associated peritoneal fibrosis. Methods Cell classification was performed on the single-cell transcriptome data of PD effluent cells from 10 PD patients （6 cases in the short-term PD group and 4 cases in the long-term PD group） obtained in our previous study, and the data of T lymphocytes were extracted to analyze the differences between two groups, and pathway enrichment analysis was also performed. At the same time, complete blood count data of 23 PD patients before and after PD were collected, and the differences in immune cell composition were compared before and after PD. Results The proportion of T lymphocytes to the total number of immune cells in the PD effluent was increased significantly with the prolongation of PD time （P < 0.05）. Differential analysis showed that TGF-β1 and other fibrosis-related signaling pathways were enriched in peritoneal T lymphocytes in the long-term PD group. Compared with the complete blood count before dialysis, the lymphocyte count in both short-term and long-term PD groups was significantly increased, but the proportion was initially increased, and subsequently decreased. Conclusions Peritoneal fibrosis caused by long-term PD treatment is closely associated with T lymphocytes. And the changes of T lymphocytes in PD effluent may be jointly caused by the peritoneal environment and alternative multiple factors. | ||
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10.3969/j.issn.0253-9802.2022.04.013 doi (DE-627)DOAJ083780726 (DE-599)DOAJf56b771eec3547c88780e29b204ba54e DE-627 ger DE-627 rakwb chi Li Yongjie, Sun Yuxiang, Lin Hongchun, Li Canming, Shang Hongli, Peng Hui verfasserin aut Single-cell transcriptome analysis reveals changes of T lymphocytes in peritoneal fluid from peritoneal dialysis patients 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To reveal the change trajectory of T lymphocytes in the dialysis effluent from peritoneal dialysis （PD） patients with the prolongation of PD time, and to explore its relationship with PD-associated peritoneal fibrosis. Methods Cell classification was performed on the single-cell transcriptome data of PD effluent cells from 10 PD patients （6 cases in the short-term PD group and 4 cases in the long-term PD group） obtained in our previous study, and the data of T lymphocytes were extracted to analyze the differences between two groups, and pathway enrichment analysis was also performed. At the same time, complete blood count data of 23 PD patients before and after PD were collected, and the differences in immune cell composition were compared before and after PD. Results The proportion of T lymphocytes to the total number of immune cells in the PD effluent was increased significantly with the prolongation of PD time （P < 0.05）. Differential analysis showed that TGF-β1 and other fibrosis-related signaling pathways were enriched in peritoneal T lymphocytes in the long-term PD group. Compared with the complete blood count before dialysis, the lymphocyte count in both short-term and long-term PD groups was significantly increased, but the proportion was initially increased, and subsequently decreased. Conclusions Peritoneal fibrosis caused by long-term PD treatment is closely associated with T lymphocytes. And the changes of T lymphocytes in PD effluent may be jointly caused by the peritoneal environment and alternative multiple factors. |single-cell transcriptome analysis|peritoneal dialysis|peritoneal fibrosis|t lymphocyte Medicine R In Xin yixue Editorial Office of Journal of New Medicine, 2022 53(2022), 4, Seite 292-296 (DE-627)DOAJ000152676 02539802 nnns volume:53 year:2022 number:4 pages:292-296 https://doi.org/10.3969/j.issn.0253-9802.2022.04.013 kostenfrei https://doaj.org/article/f56b771eec3547c88780e29b204ba54e kostenfrei https://www.xinyixue.cn/fileup/0253-9802/PDF/1652688763780-1356779730.pdf kostenfrei https://doaj.org/toc/0253-9802 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 53 2022 4 292-296 |
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10.3969/j.issn.0253-9802.2022.04.013 doi (DE-627)DOAJ083780726 (DE-599)DOAJf56b771eec3547c88780e29b204ba54e DE-627 ger DE-627 rakwb chi Li Yongjie, Sun Yuxiang, Lin Hongchun, Li Canming, Shang Hongli, Peng Hui verfasserin aut Single-cell transcriptome analysis reveals changes of T lymphocytes in peritoneal fluid from peritoneal dialysis patients 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To reveal the change trajectory of T lymphocytes in the dialysis effluent from peritoneal dialysis （PD） patients with the prolongation of PD time, and to explore its relationship with PD-associated peritoneal fibrosis. Methods Cell classification was performed on the single-cell transcriptome data of PD effluent cells from 10 PD patients （6 cases in the short-term PD group and 4 cases in the long-term PD group） obtained in our previous study, and the data of T lymphocytes were extracted to analyze the differences between two groups, and pathway enrichment analysis was also performed. At the same time, complete blood count data of 23 PD patients before and after PD were collected, and the differences in immune cell composition were compared before and after PD. Results The proportion of T lymphocytes to the total number of immune cells in the PD effluent was increased significantly with the prolongation of PD time （P < 0.05）. Differential analysis showed that TGF-β1 and other fibrosis-related signaling pathways were enriched in peritoneal T lymphocytes in the long-term PD group. Compared with the complete blood count before dialysis, the lymphocyte count in both short-term and long-term PD groups was significantly increased, but the proportion was initially increased, and subsequently decreased. Conclusions Peritoneal fibrosis caused by long-term PD treatment is closely associated with T lymphocytes. And the changes of T lymphocytes in PD effluent may be jointly caused by the peritoneal environment and alternative multiple factors. |single-cell transcriptome analysis|peritoneal dialysis|peritoneal fibrosis|t lymphocyte Medicine R In Xin yixue Editorial Office of Journal of New Medicine, 2022 53(2022), 4, Seite 292-296 (DE-627)DOAJ000152676 02539802 nnns volume:53 year:2022 number:4 pages:292-296 https://doi.org/10.3969/j.issn.0253-9802.2022.04.013 kostenfrei https://doaj.org/article/f56b771eec3547c88780e29b204ba54e kostenfrei https://www.xinyixue.cn/fileup/0253-9802/PDF/1652688763780-1356779730.pdf kostenfrei https://doaj.org/toc/0253-9802 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 53 2022 4 292-296 |
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10.3969/j.issn.0253-9802.2022.04.013 doi (DE-627)DOAJ083780726 (DE-599)DOAJf56b771eec3547c88780e29b204ba54e DE-627 ger DE-627 rakwb chi Li Yongjie, Sun Yuxiang, Lin Hongchun, Li Canming, Shang Hongli, Peng Hui verfasserin aut Single-cell transcriptome analysis reveals changes of T lymphocytes in peritoneal fluid from peritoneal dialysis patients 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To reveal the change trajectory of T lymphocytes in the dialysis effluent from peritoneal dialysis （PD） patients with the prolongation of PD time, and to explore its relationship with PD-associated peritoneal fibrosis. Methods Cell classification was performed on the single-cell transcriptome data of PD effluent cells from 10 PD patients （6 cases in the short-term PD group and 4 cases in the long-term PD group） obtained in our previous study, and the data of T lymphocytes were extracted to analyze the differences between two groups, and pathway enrichment analysis was also performed. At the same time, complete blood count data of 23 PD patients before and after PD were collected, and the differences in immune cell composition were compared before and after PD. Results The proportion of T lymphocytes to the total number of immune cells in the PD effluent was increased significantly with the prolongation of PD time （P < 0.05）. Differential analysis showed that TGF-β1 and other fibrosis-related signaling pathways were enriched in peritoneal T lymphocytes in the long-term PD group. Compared with the complete blood count before dialysis, the lymphocyte count in both short-term and long-term PD groups was significantly increased, but the proportion was initially increased, and subsequently decreased. Conclusions Peritoneal fibrosis caused by long-term PD treatment is closely associated with T lymphocytes. And the changes of T lymphocytes in PD effluent may be jointly caused by the peritoneal environment and alternative multiple factors. |single-cell transcriptome analysis|peritoneal dialysis|peritoneal fibrosis|t lymphocyte Medicine R In Xin yixue Editorial Office of Journal of New Medicine, 2022 53(2022), 4, Seite 292-296 (DE-627)DOAJ000152676 02539802 nnns volume:53 year:2022 number:4 pages:292-296 https://doi.org/10.3969/j.issn.0253-9802.2022.04.013 kostenfrei https://doaj.org/article/f56b771eec3547c88780e29b204ba54e kostenfrei https://www.xinyixue.cn/fileup/0253-9802/PDF/1652688763780-1356779730.pdf kostenfrei https://doaj.org/toc/0253-9802 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 53 2022 4 292-296 |
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10.3969/j.issn.0253-9802.2022.04.013 doi (DE-627)DOAJ083780726 (DE-599)DOAJf56b771eec3547c88780e29b204ba54e DE-627 ger DE-627 rakwb chi Li Yongjie, Sun Yuxiang, Lin Hongchun, Li Canming, Shang Hongli, Peng Hui verfasserin aut Single-cell transcriptome analysis reveals changes of T lymphocytes in peritoneal fluid from peritoneal dialysis patients 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To reveal the change trajectory of T lymphocytes in the dialysis effluent from peritoneal dialysis （PD） patients with the prolongation of PD time, and to explore its relationship with PD-associated peritoneal fibrosis. Methods Cell classification was performed on the single-cell transcriptome data of PD effluent cells from 10 PD patients （6 cases in the short-term PD group and 4 cases in the long-term PD group） obtained in our previous study, and the data of T lymphocytes were extracted to analyze the differences between two groups, and pathway enrichment analysis was also performed. At the same time, complete blood count data of 23 PD patients before and after PD were collected, and the differences in immune cell composition were compared before and after PD. Results The proportion of T lymphocytes to the total number of immune cells in the PD effluent was increased significantly with the prolongation of PD time （P < 0.05）. Differential analysis showed that TGF-β1 and other fibrosis-related signaling pathways were enriched in peritoneal T lymphocytes in the long-term PD group. Compared with the complete blood count before dialysis, the lymphocyte count in both short-term and long-term PD groups was significantly increased, but the proportion was initially increased, and subsequently decreased. Conclusions Peritoneal fibrosis caused by long-term PD treatment is closely associated with T lymphocytes. And the changes of T lymphocytes in PD effluent may be jointly caused by the peritoneal environment and alternative multiple factors. |single-cell transcriptome analysis|peritoneal dialysis|peritoneal fibrosis|t lymphocyte Medicine R In Xin yixue Editorial Office of Journal of New Medicine, 2022 53(2022), 4, Seite 292-296 (DE-627)DOAJ000152676 02539802 nnns volume:53 year:2022 number:4 pages:292-296 https://doi.org/10.3969/j.issn.0253-9802.2022.04.013 kostenfrei https://doaj.org/article/f56b771eec3547c88780e29b204ba54e kostenfrei https://www.xinyixue.cn/fileup/0253-9802/PDF/1652688763780-1356779730.pdf kostenfrei https://doaj.org/toc/0253-9802 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 53 2022 4 292-296 |
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10.3969/j.issn.0253-9802.2022.04.013 doi (DE-627)DOAJ083780726 (DE-599)DOAJf56b771eec3547c88780e29b204ba54e DE-627 ger DE-627 rakwb chi Li Yongjie, Sun Yuxiang, Lin Hongchun, Li Canming, Shang Hongli, Peng Hui verfasserin aut Single-cell transcriptome analysis reveals changes of T lymphocytes in peritoneal fluid from peritoneal dialysis patients 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To reveal the change trajectory of T lymphocytes in the dialysis effluent from peritoneal dialysis （PD） patients with the prolongation of PD time, and to explore its relationship with PD-associated peritoneal fibrosis. Methods Cell classification was performed on the single-cell transcriptome data of PD effluent cells from 10 PD patients （6 cases in the short-term PD group and 4 cases in the long-term PD group） obtained in our previous study, and the data of T lymphocytes were extracted to analyze the differences between two groups, and pathway enrichment analysis was also performed. At the same time, complete blood count data of 23 PD patients before and after PD were collected, and the differences in immune cell composition were compared before and after PD. Results The proportion of T lymphocytes to the total number of immune cells in the PD effluent was increased significantly with the prolongation of PD time （P < 0.05）. Differential analysis showed that TGF-β1 and other fibrosis-related signaling pathways were enriched in peritoneal T lymphocytes in the long-term PD group. Compared with the complete blood count before dialysis, the lymphocyte count in both short-term and long-term PD groups was significantly increased, but the proportion was initially increased, and subsequently decreased. Conclusions Peritoneal fibrosis caused by long-term PD treatment is closely associated with T lymphocytes. And the changes of T lymphocytes in PD effluent may be jointly caused by the peritoneal environment and alternative multiple factors. |single-cell transcriptome analysis|peritoneal dialysis|peritoneal fibrosis|t lymphocyte Medicine R In Xin yixue Editorial Office of Journal of New Medicine, 2022 53(2022), 4, Seite 292-296 (DE-627)DOAJ000152676 02539802 nnns volume:53 year:2022 number:4 pages:292-296 https://doi.org/10.3969/j.issn.0253-9802.2022.04.013 kostenfrei https://doaj.org/article/f56b771eec3547c88780e29b204ba54e kostenfrei https://www.xinyixue.cn/fileup/0253-9802/PDF/1652688763780-1356779730.pdf kostenfrei https://doaj.org/toc/0253-9802 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 53 2022 4 292-296 |
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Single-cell transcriptome analysis reveals changes of T lymphocytes in peritoneal fluid from peritoneal dialysis patients |
| abstract |
Objective To reveal the change trajectory of T lymphocytes in the dialysis effluent from peritoneal dialysis （PD） patients with the prolongation of PD time, and to explore its relationship with PD-associated peritoneal fibrosis. Methods Cell classification was performed on the single-cell transcriptome data of PD effluent cells from 10 PD patients （6 cases in the short-term PD group and 4 cases in the long-term PD group） obtained in our previous study, and the data of T lymphocytes were extracted to analyze the differences between two groups, and pathway enrichment analysis was also performed. At the same time, complete blood count data of 23 PD patients before and after PD were collected, and the differences in immune cell composition were compared before and after PD. Results The proportion of T lymphocytes to the total number of immune cells in the PD effluent was increased significantly with the prolongation of PD time （P < 0.05）. Differential analysis showed that TGF-β1 and other fibrosis-related signaling pathways were enriched in peritoneal T lymphocytes in the long-term PD group. Compared with the complete blood count before dialysis, the lymphocyte count in both short-term and long-term PD groups was significantly increased, but the proportion was initially increased, and subsequently decreased. Conclusions Peritoneal fibrosis caused by long-term PD treatment is closely associated with T lymphocytes. And the changes of T lymphocytes in PD effluent may be jointly caused by the peritoneal environment and alternative multiple factors. |
| abstractGer |
Objective To reveal the change trajectory of T lymphocytes in the dialysis effluent from peritoneal dialysis （PD） patients with the prolongation of PD time, and to explore its relationship with PD-associated peritoneal fibrosis. Methods Cell classification was performed on the single-cell transcriptome data of PD effluent cells from 10 PD patients （6 cases in the short-term PD group and 4 cases in the long-term PD group） obtained in our previous study, and the data of T lymphocytes were extracted to analyze the differences between two groups, and pathway enrichment analysis was also performed. At the same time, complete blood count data of 23 PD patients before and after PD were collected, and the differences in immune cell composition were compared before and after PD. Results The proportion of T lymphocytes to the total number of immune cells in the PD effluent was increased significantly with the prolongation of PD time （P < 0.05）. Differential analysis showed that TGF-β1 and other fibrosis-related signaling pathways were enriched in peritoneal T lymphocytes in the long-term PD group. Compared with the complete blood count before dialysis, the lymphocyte count in both short-term and long-term PD groups was significantly increased, but the proportion was initially increased, and subsequently decreased. Conclusions Peritoneal fibrosis caused by long-term PD treatment is closely associated with T lymphocytes. And the changes of T lymphocytes in PD effluent may be jointly caused by the peritoneal environment and alternative multiple factors. |
| abstract_unstemmed |
Objective To reveal the change trajectory of T lymphocytes in the dialysis effluent from peritoneal dialysis （PD） patients with the prolongation of PD time, and to explore its relationship with PD-associated peritoneal fibrosis. Methods Cell classification was performed on the single-cell transcriptome data of PD effluent cells from 10 PD patients （6 cases in the short-term PD group and 4 cases in the long-term PD group） obtained in our previous study, and the data of T lymphocytes were extracted to analyze the differences between two groups, and pathway enrichment analysis was also performed. At the same time, complete blood count data of 23 PD patients before and after PD were collected, and the differences in immune cell composition were compared before and after PD. Results The proportion of T lymphocytes to the total number of immune cells in the PD effluent was increased significantly with the prolongation of PD time （P < 0.05）. Differential analysis showed that TGF-β1 and other fibrosis-related signaling pathways were enriched in peritoneal T lymphocytes in the long-term PD group. Compared with the complete blood count before dialysis, the lymphocyte count in both short-term and long-term PD groups was significantly increased, but the proportion was initially increased, and subsequently decreased. Conclusions Peritoneal fibrosis caused by long-term PD treatment is closely associated with T lymphocytes. And the changes of T lymphocytes in PD effluent may be jointly caused by the peritoneal environment and alternative multiple factors. |
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Single-cell transcriptome analysis reveals changes of T lymphocytes in peritoneal fluid from peritoneal dialysis patients |
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https://doi.org/10.3969/j.issn.0253-9802.2022.04.013 https://doaj.org/article/f56b771eec3547c88780e29b204ba54e https://www.xinyixue.cn/fileup/0253-9802/PDF/1652688763780-1356779730.pdf https://doaj.org/toc/0253-9802 |
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10.3969/j.issn.0253-9802.2022.04.013 |
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2024-07-03T19:24:48.439Z |
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