Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and <i<Bemisia tabaci</i< in the Comoros Islands

A comprehensive assessment of cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) was carried out in Comoros where cassava yield (5.7 t/ha) is significantly below the African average (8.6 t/ha) largely due to virus diseases. Observations from 66 sites across the Comoros Islands of M...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Rudolph Rufini Shirima [verfasserIn] Everlyne Nafula Wosula [verfasserIn] Abdou Azali Hamza [verfasserIn] Nobataine Ali Mohammed [verfasserIn] Hadji Mouigni [verfasserIn] Salima Nouhou [verfasserIn] Naima Mmadi Mchinda [verfasserIn] Gloria Ceasar [verfasserIn] Massoud Amour [verfasserIn] Emmanuel Njukwe [verfasserIn] James Peter Legg [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	surveillance CBSD CMD CBSIs CMBs

Übergeordnetes Werk:	In: Viruses - MDPI AG, 2009, 14(2022), 10, p 2165
Übergeordnetes Werk:	volume:14 ; year:2022 ; number:10, p 2165

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/v14102165

Katalog-ID:	DOAJ083904093

Internformat


LEADER	01000caa a22002652 4500
001	DOAJ083904093
003	DE-627
005	20240414173447.0
007	cr uuu---uuuuu
008	230311s2022 xx \|\|\|\|\|o 00\| \|\|eng c
024	7		\|a 10.3390/v14102165 \|2 doi
035			\|a (DE-627)DOAJ083904093
035			\|a (DE-599)DOAJ1c88be78da67463eb3fc4d7f07b4e209
040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a eng
050		0	\|a QR1-502
100	0		\|a Rudolph Rufini Shirima \|e verfasserin \|4 aut
245	1	0	\|a Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and <i<Bemisia tabaci</i< in the Comoros Islands
264		1	\|c 2022
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
520			\|a A comprehensive assessment of cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) was carried out in Comoros where cassava yield (5.7 t/ha) is significantly below the African average (8.6 t/ha) largely due to virus diseases. Observations from 66 sites across the Comoros Islands of Mwali, Ngazidja, and Ndzwani revealed that 83.3% of cassava fields had foliar symptoms of CBSD compared with 95.5% for CMD. Molecular diagnostics confirmed the presence of both cassava brown streak ipomoviruses (CBSIs) and cassava mosaic begomoviruses (CMBs). Although real-time RT-PCR only detected the presence of one CBSI species (<i<Cassava brown streak virus</i<, CBSV) the second species (<i<Ugandan cassava brown streak virus</i<, UCBSV) was identified using next-generation high-throughput sequencing. Both PCR and HTS detected the presence of East African cassava mosaic virus (EACMV). African cassava mosaic virus was not detected in any of the samples. Four whitefly species were identified from a sample of 131 specimens: <i<Bemisia tabaci</i<, <i<B. afer</i<, <i<Aleurodicus dispersus</i<, and <i<Paraleyrodes bondari</i<. Cassava <i<B. tabaci</i< comprised two mitotypes: SSA1-SG2 (89%) and SSA1-SG3 (11%). KASP SNP genotyping categorized 82% of cassava <i<B. tabaci</i< as haplogroup SSA-ESA. This knowledge will provide an important base for developing and deploying effective management strategies for cassava viruses and their vectors.
650		4	\|a surveillance
650		4	\|a CBSD
650		4	\|a CMD
650		4	\|a CBSIs
650		4	\|a CMBs
650		4	\|a <i<Bemisia tabaci</i<
653		0	\|a Microbiology
700	0		\|a Everlyne Nafula Wosula \|e verfasserin \|4 aut
700	0		\|a Abdou Azali Hamza \|e verfasserin \|4 aut
700	0		\|a Nobataine Ali Mohammed \|e verfasserin \|4 aut
700	0		\|a Hadji Mouigni \|e verfasserin \|4 aut
700	0		\|a Salima Nouhou \|e verfasserin \|4 aut
700	0		\|a Naima Mmadi Mchinda \|e verfasserin \|4 aut
700	0		\|a Gloria Ceasar \|e verfasserin \|4 aut
700	0		\|a Massoud Amour \|e verfasserin \|4 aut
700	0		\|a Emmanuel Njukwe \|e verfasserin \|4 aut
700	0		\|a James Peter Legg \|e verfasserin \|4 aut
773	0	8	\|i In \|t Viruses \|d MDPI AG, 2009 \|g 14(2022), 10, p 2165 \|w (DE-627)609775871 \|w (DE-600)2516098-9 \|x 19994915 \|7 nnns
773	1	8	\|g volume:14 \|g year:2022 \|g number:10, p 2165
856	4	0	\|u https://doi.org/10.3390/v14102165 \|z kostenfrei
856	4	0	\|u https://doaj.org/article/1c88be78da67463eb3fc4d7f07b4e209 \|z kostenfrei
856	4	0	\|u https://www.mdpi.com/1999-4915/14/10/2165 \|z kostenfrei
856	4	2	\|u https://doaj.org/toc/1999-4915 \|y Journal toc \|z kostenfrei
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_DOAJ
912			\|a GBV_ILN_11
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_95
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_206
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 14 \|j 2022 \|e 10, p 2165

Indexfelder

author_variant	r r s rrs e n w enw a a h aah n a m nam h m hm s n sn n m m nmm g c gc m a ma e n en j p l jpl
matchkey_str	article:19994915:2022----::pdmooiaaayiocsaaoacnbontekiessnieiit
hierarchy_sort_str	2022
callnumber-subject-code	QR
publishDate	2022
allfields	10.3390/v14102165 doi (DE-627)DOAJ083904093 (DE-599)DOAJ1c88be78da67463eb3fc4d7f07b4e209 DE-627 ger DE-627 rakwb eng QR1-502 Rudolph Rufini Shirima verfasserin aut Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and <i<Bemisia tabaci</i< in the Comoros Islands 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A comprehensive assessment of cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) was carried out in Comoros where cassava yield (5.7 t/ha) is significantly below the African average (8.6 t/ha) largely due to virus diseases. Observations from 66 sites across the Comoros Islands of Mwali, Ngazidja, and Ndzwani revealed that 83.3% of cassava fields had foliar symptoms of CBSD compared with 95.5% for CMD. Molecular diagnostics confirmed the presence of both cassava brown streak ipomoviruses (CBSIs) and cassava mosaic begomoviruses (CMBs). Although real-time RT-PCR only detected the presence of one CBSI species (<i<Cassava brown streak virus</i<, CBSV) the second species (<i<Ugandan cassava brown streak virus</i<, UCBSV) was identified using next-generation high-throughput sequencing. Both PCR and HTS detected the presence of East African cassava mosaic virus (EACMV). African cassava mosaic virus was not detected in any of the samples. Four whitefly species were identified from a sample of 131 specimens: <i<Bemisia tabaci</i<, <i<B. afer</i<, <i<Aleurodicus dispersus</i<, and <i<Paraleyrodes bondari</i<. Cassava <i<B. tabaci</i< comprised two mitotypes: SSA1-SG2 (89%) and SSA1-SG3 (11%). KASP SNP genotyping categorized 82% of cassava <i<B. tabaci</i< as haplogroup SSA-ESA. This knowledge will provide an important base for developing and deploying effective management strategies for cassava viruses and their vectors. surveillance CBSD CMD CBSIs CMBs <i<Bemisia tabaci</i< Microbiology Everlyne Nafula Wosula verfasserin aut Abdou Azali Hamza verfasserin aut Nobataine Ali Mohammed verfasserin aut Hadji Mouigni verfasserin aut Salima Nouhou verfasserin aut Naima Mmadi Mchinda verfasserin aut Gloria Ceasar verfasserin aut Massoud Amour verfasserin aut Emmanuel Njukwe verfasserin aut James Peter Legg verfasserin aut In Viruses MDPI AG, 2009 14(2022), 10, p 2165 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:14 year:2022 number:10, p 2165 https://doi.org/10.3390/v14102165 kostenfrei https://doaj.org/article/1c88be78da67463eb3fc4d7f07b4e209 kostenfrei https://www.mdpi.com/1999-4915/14/10/2165 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 10, p 2165
spelling	10.3390/v14102165 doi (DE-627)DOAJ083904093 (DE-599)DOAJ1c88be78da67463eb3fc4d7f07b4e209 DE-627 ger DE-627 rakwb eng QR1-502 Rudolph Rufini Shirima verfasserin aut Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and <i<Bemisia tabaci</i< in the Comoros Islands 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A comprehensive assessment of cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) was carried out in Comoros where cassava yield (5.7 t/ha) is significantly below the African average (8.6 t/ha) largely due to virus diseases. Observations from 66 sites across the Comoros Islands of Mwali, Ngazidja, and Ndzwani revealed that 83.3% of cassava fields had foliar symptoms of CBSD compared with 95.5% for CMD. Molecular diagnostics confirmed the presence of both cassava brown streak ipomoviruses (CBSIs) and cassava mosaic begomoviruses (CMBs). Although real-time RT-PCR only detected the presence of one CBSI species (<i<Cassava brown streak virus</i<, CBSV) the second species (<i<Ugandan cassava brown streak virus</i<, UCBSV) was identified using next-generation high-throughput sequencing. Both PCR and HTS detected the presence of East African cassava mosaic virus (EACMV). African cassava mosaic virus was not detected in any of the samples. Four whitefly species were identified from a sample of 131 specimens: <i<Bemisia tabaci</i<, <i<B. afer</i<, <i<Aleurodicus dispersus</i<, and <i<Paraleyrodes bondari</i<. Cassava <i<B. tabaci</i< comprised two mitotypes: SSA1-SG2 (89%) and SSA1-SG3 (11%). KASP SNP genotyping categorized 82% of cassava <i<B. tabaci</i< as haplogroup SSA-ESA. This knowledge will provide an important base for developing and deploying effective management strategies for cassava viruses and their vectors. surveillance CBSD CMD CBSIs CMBs <i<Bemisia tabaci</i< Microbiology Everlyne Nafula Wosula verfasserin aut Abdou Azali Hamza verfasserin aut Nobataine Ali Mohammed verfasserin aut Hadji Mouigni verfasserin aut Salima Nouhou verfasserin aut Naima Mmadi Mchinda verfasserin aut Gloria Ceasar verfasserin aut Massoud Amour verfasserin aut Emmanuel Njukwe verfasserin aut James Peter Legg verfasserin aut In Viruses MDPI AG, 2009 14(2022), 10, p 2165 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:14 year:2022 number:10, p 2165 https://doi.org/10.3390/v14102165 kostenfrei https://doaj.org/article/1c88be78da67463eb3fc4d7f07b4e209 kostenfrei https://www.mdpi.com/1999-4915/14/10/2165 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 10, p 2165
allfields_unstemmed	10.3390/v14102165 doi (DE-627)DOAJ083904093 (DE-599)DOAJ1c88be78da67463eb3fc4d7f07b4e209 DE-627 ger DE-627 rakwb eng QR1-502 Rudolph Rufini Shirima verfasserin aut Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and <i<Bemisia tabaci</i< in the Comoros Islands 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A comprehensive assessment of cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) was carried out in Comoros where cassava yield (5.7 t/ha) is significantly below the African average (8.6 t/ha) largely due to virus diseases. Observations from 66 sites across the Comoros Islands of Mwali, Ngazidja, and Ndzwani revealed that 83.3% of cassava fields had foliar symptoms of CBSD compared with 95.5% for CMD. Molecular diagnostics confirmed the presence of both cassava brown streak ipomoviruses (CBSIs) and cassava mosaic begomoviruses (CMBs). Although real-time RT-PCR only detected the presence of one CBSI species (<i<Cassava brown streak virus</i<, CBSV) the second species (<i<Ugandan cassava brown streak virus</i<, UCBSV) was identified using next-generation high-throughput sequencing. Both PCR and HTS detected the presence of East African cassava mosaic virus (EACMV). African cassava mosaic virus was not detected in any of the samples. Four whitefly species were identified from a sample of 131 specimens: <i<Bemisia tabaci</i<, <i<B. afer</i<, <i<Aleurodicus dispersus</i<, and <i<Paraleyrodes bondari</i<. Cassava <i<B. tabaci</i< comprised two mitotypes: SSA1-SG2 (89%) and SSA1-SG3 (11%). KASP SNP genotyping categorized 82% of cassava <i<B. tabaci</i< as haplogroup SSA-ESA. This knowledge will provide an important base for developing and deploying effective management strategies for cassava viruses and their vectors. surveillance CBSD CMD CBSIs CMBs <i<Bemisia tabaci</i< Microbiology Everlyne Nafula Wosula verfasserin aut Abdou Azali Hamza verfasserin aut Nobataine Ali Mohammed verfasserin aut Hadji Mouigni verfasserin aut Salima Nouhou verfasserin aut Naima Mmadi Mchinda verfasserin aut Gloria Ceasar verfasserin aut Massoud Amour verfasserin aut Emmanuel Njukwe verfasserin aut James Peter Legg verfasserin aut In Viruses MDPI AG, 2009 14(2022), 10, p 2165 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:14 year:2022 number:10, p 2165 https://doi.org/10.3390/v14102165 kostenfrei https://doaj.org/article/1c88be78da67463eb3fc4d7f07b4e209 kostenfrei https://www.mdpi.com/1999-4915/14/10/2165 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 10, p 2165
allfieldsGer	10.3390/v14102165 doi (DE-627)DOAJ083904093 (DE-599)DOAJ1c88be78da67463eb3fc4d7f07b4e209 DE-627 ger DE-627 rakwb eng QR1-502 Rudolph Rufini Shirima verfasserin aut Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and <i<Bemisia tabaci</i< in the Comoros Islands 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A comprehensive assessment of cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) was carried out in Comoros where cassava yield (5.7 t/ha) is significantly below the African average (8.6 t/ha) largely due to virus diseases. Observations from 66 sites across the Comoros Islands of Mwali, Ngazidja, and Ndzwani revealed that 83.3% of cassava fields had foliar symptoms of CBSD compared with 95.5% for CMD. Molecular diagnostics confirmed the presence of both cassava brown streak ipomoviruses (CBSIs) and cassava mosaic begomoviruses (CMBs). Although real-time RT-PCR only detected the presence of one CBSI species (<i<Cassava brown streak virus</i<, CBSV) the second species (<i<Ugandan cassava brown streak virus</i<, UCBSV) was identified using next-generation high-throughput sequencing. Both PCR and HTS detected the presence of East African cassava mosaic virus (EACMV). African cassava mosaic virus was not detected in any of the samples. Four whitefly species were identified from a sample of 131 specimens: <i<Bemisia tabaci</i<, <i<B. afer</i<, <i<Aleurodicus dispersus</i<, and <i<Paraleyrodes bondari</i<. Cassava <i<B. tabaci</i< comprised two mitotypes: SSA1-SG2 (89%) and SSA1-SG3 (11%). KASP SNP genotyping categorized 82% of cassava <i<B. tabaci</i< as haplogroup SSA-ESA. This knowledge will provide an important base for developing and deploying effective management strategies for cassava viruses and their vectors. surveillance CBSD CMD CBSIs CMBs <i<Bemisia tabaci</i< Microbiology Everlyne Nafula Wosula verfasserin aut Abdou Azali Hamza verfasserin aut Nobataine Ali Mohammed verfasserin aut Hadji Mouigni verfasserin aut Salima Nouhou verfasserin aut Naima Mmadi Mchinda verfasserin aut Gloria Ceasar verfasserin aut Massoud Amour verfasserin aut Emmanuel Njukwe verfasserin aut James Peter Legg verfasserin aut In Viruses MDPI AG, 2009 14(2022), 10, p 2165 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:14 year:2022 number:10, p 2165 https://doi.org/10.3390/v14102165 kostenfrei https://doaj.org/article/1c88be78da67463eb3fc4d7f07b4e209 kostenfrei https://www.mdpi.com/1999-4915/14/10/2165 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 10, p 2165
allfieldsSound	10.3390/v14102165 doi (DE-627)DOAJ083904093 (DE-599)DOAJ1c88be78da67463eb3fc4d7f07b4e209 DE-627 ger DE-627 rakwb eng QR1-502 Rudolph Rufini Shirima verfasserin aut Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and <i<Bemisia tabaci</i< in the Comoros Islands 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A comprehensive assessment of cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) was carried out in Comoros where cassava yield (5.7 t/ha) is significantly below the African average (8.6 t/ha) largely due to virus diseases. Observations from 66 sites across the Comoros Islands of Mwali, Ngazidja, and Ndzwani revealed that 83.3% of cassava fields had foliar symptoms of CBSD compared with 95.5% for CMD. Molecular diagnostics confirmed the presence of both cassava brown streak ipomoviruses (CBSIs) and cassava mosaic begomoviruses (CMBs). Although real-time RT-PCR only detected the presence of one CBSI species (<i<Cassava brown streak virus</i<, CBSV) the second species (<i<Ugandan cassava brown streak virus</i<, UCBSV) was identified using next-generation high-throughput sequencing. Both PCR and HTS detected the presence of East African cassava mosaic virus (EACMV). African cassava mosaic virus was not detected in any of the samples. Four whitefly species were identified from a sample of 131 specimens: <i<Bemisia tabaci</i<, <i<B. afer</i<, <i<Aleurodicus dispersus</i<, and <i<Paraleyrodes bondari</i<. Cassava <i<B. tabaci</i< comprised two mitotypes: SSA1-SG2 (89%) and SSA1-SG3 (11%). KASP SNP genotyping categorized 82% of cassava <i<B. tabaci</i< as haplogroup SSA-ESA. This knowledge will provide an important base for developing and deploying effective management strategies for cassava viruses and their vectors. surveillance CBSD CMD CBSIs CMBs <i<Bemisia tabaci</i< Microbiology Everlyne Nafula Wosula verfasserin aut Abdou Azali Hamza verfasserin aut Nobataine Ali Mohammed verfasserin aut Hadji Mouigni verfasserin aut Salima Nouhou verfasserin aut Naima Mmadi Mchinda verfasserin aut Gloria Ceasar verfasserin aut Massoud Amour verfasserin aut Emmanuel Njukwe verfasserin aut James Peter Legg verfasserin aut In Viruses MDPI AG, 2009 14(2022), 10, p 2165 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:14 year:2022 number:10, p 2165 https://doi.org/10.3390/v14102165 kostenfrei https://doaj.org/article/1c88be78da67463eb3fc4d7f07b4e209 kostenfrei https://www.mdpi.com/1999-4915/14/10/2165 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 10, p 2165
language	English
source	In Viruses 14(2022), 10, p 2165 volume:14 year:2022 number:10, p 2165
sourceStr	In Viruses 14(2022), 10, p 2165 volume:14 year:2022 number:10, p 2165
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	surveillance CBSD CMD CBSIs CMBs <i<Bemisia tabaci</i< Microbiology
isfreeaccess_bool	true
container_title	Viruses
authorswithroles_txt_mv	Rudolph Rufini Shirima @@aut@@ Everlyne Nafula Wosula @@aut@@ Abdou Azali Hamza @@aut@@ Nobataine Ali Mohammed @@aut@@ Hadji Mouigni @@aut@@ Salima Nouhou @@aut@@ Naima Mmadi Mchinda @@aut@@ Gloria Ceasar @@aut@@ Massoud Amour @@aut@@ Emmanuel Njukwe @@aut@@ James Peter Legg @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	609775871
id	DOAJ083904093
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ083904093</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240414173447.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230311s2022 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3390/v14102165</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ083904093</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ1c88be78da67463eb3fc4d7f07b4e209</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QR1-502</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Rudolph Rufini Shirima</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and <i<Bemisia tabaci</i< in the Comoros Islands</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2022</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">A comprehensive assessment of cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) was carried out in Comoros where cassava yield (5.7 t/ha) is significantly below the African average (8.6 t/ha) largely due to virus diseases. Observations from 66 sites across the Comoros Islands of Mwali, Ngazidja, and Ndzwani revealed that 83.3% of cassava fields had foliar symptoms of CBSD compared with 95.5% for CMD. Molecular diagnostics confirmed the presence of both cassava brown streak ipomoviruses (CBSIs) and cassava mosaic begomoviruses (CMBs). Although real-time RT-PCR only detected the presence of one CBSI species (<i<Cassava brown streak virus</i<, CBSV) the second species (<i<Ugandan cassava brown streak virus</i<, UCBSV) was identified using next-generation high-throughput sequencing. Both PCR and HTS detected the presence of East African cassava mosaic virus (EACMV). African cassava mosaic virus was not detected in any of the samples. Four whitefly species were identified from a sample of 131 specimens: <i<Bemisia tabaci</i<, <i<B. afer</i<, <i<Aleurodicus dispersus</i<, and <i<Paraleyrodes bondari</i<. Cassava <i<B. tabaci</i< comprised two mitotypes: SSA1-SG2 (89%) and SSA1-SG3 (11%). KASP SNP genotyping categorized 82% of cassava <i<B. tabaci</i< as haplogroup SSA-ESA. This knowledge will provide an important base for developing and deploying effective management strategies for cassava viruses and their vectors.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">surveillance</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">CBSD</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">CMD</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">CBSIs</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">CMBs</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><i<Bemisia tabaci</i<</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Microbiology</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Everlyne Nafula Wosula</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Abdou Azali Hamza</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Nobataine Ali Mohammed</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Hadji Mouigni</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Salima Nouhou</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Naima Mmadi Mchinda</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Gloria Ceasar</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Massoud Amour</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Emmanuel Njukwe</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">James Peter Legg</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Viruses</subfield><subfield code="d">MDPI AG, 2009</subfield><subfield code="g">14(2022), 10, p 2165</subfield><subfield code="w">(DE-627)609775871</subfield><subfield code="w">(DE-600)2516098-9</subfield><subfield code="x">19994915</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:14</subfield><subfield code="g">year:2022</subfield><subfield code="g">number:10, p 2165</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.3390/v14102165</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/1c88be78da67463eb3fc4d7f07b4e209</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.mdpi.com/1999-4915/14/10/2165</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1999-4915</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_11</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_224</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_370</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">14</subfield><subfield code="j">2022</subfield><subfield code="e">10, p 2165</subfield></datafield></record></collection>
callnumber-first	Q - Science
author	Rudolph Rufini Shirima
spellingShingle	Rudolph Rufini Shirima misc QR1-502 misc surveillance misc CBSD misc CMD misc CBSIs misc CMBs misc <i<Bemisia tabaci</i< misc Microbiology Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and <i<Bemisia tabaci</i< in the Comoros Islands
authorStr	Rudolph Rufini Shirima
ppnlink_with_tag_str_mv	@@773@@(DE-627)609775871
format	electronic Article
delete_txt_mv	keep
author_role	aut aut aut aut aut aut aut aut aut aut aut
collection	DOAJ
remote_str	true
callnumber-label	QR1-502
illustrated	Not Illustrated
issn	19994915
topic_title	QR1-502 Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and <i<Bemisia tabaci</i< in the Comoros Islands surveillance CBSD CMD CBSIs CMBs <i<Bemisia tabaci</i<
topic	misc QR1-502 misc surveillance misc CBSD misc CMD misc CBSIs misc CMBs misc <i<Bemisia tabaci</i< misc Microbiology
topic_unstemmed	misc QR1-502 misc surveillance misc CBSD misc CMD misc CBSIs misc CMBs misc <i<Bemisia tabaci</i< misc Microbiology
topic_browse	misc QR1-502 misc surveillance misc CBSD misc CMD misc CBSIs misc CMBs misc <i<Bemisia tabaci</i< misc Microbiology
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
format_main_str_mv	Text Zeitschrift/Artikel
carriertype_str_mv	cr
hierarchy_parent_title	Viruses
hierarchy_parent_id	609775871
hierarchy_top_title	Viruses
isfreeaccess_txt	true
familylinks_str_mv	(DE-627)609775871 (DE-600)2516098-9
title	Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and <i<Bemisia tabaci</i< in the Comoros Islands
ctrlnum	(DE-627)DOAJ083904093 (DE-599)DOAJ1c88be78da67463eb3fc4d7f07b4e209
title_full	Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and <i<Bemisia tabaci</i< in the Comoros Islands
author_sort	Rudolph Rufini Shirima
journal	Viruses
journalStr	Viruses
callnumber-first-code	Q
lang_code	eng
isOA_bool	true
recordtype	marc
publishDateSort	2022
contenttype_str_mv	txt
author_browse	Rudolph Rufini Shirima Everlyne Nafula Wosula Abdou Azali Hamza Nobataine Ali Mohammed Hadji Mouigni Salima Nouhou Naima Mmadi Mchinda Gloria Ceasar Massoud Amour Emmanuel Njukwe James Peter Legg
container_volume	14
class	QR1-502
format_se	Elektronische Aufsätze
author-letter	Rudolph Rufini Shirima
doi_str_mv	10.3390/v14102165
author2-role	verfasserin
title_sort	epidemiological analysis of cassava mosaic and brown streak diseases, and <i<bemisia tabaci</i< in the comoros islands
callnumber	QR1-502
title_auth	Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and <i<Bemisia tabaci</i< in the Comoros Islands
abstract	A comprehensive assessment of cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) was carried out in Comoros where cassava yield (5.7 t/ha) is significantly below the African average (8.6 t/ha) largely due to virus diseases. Observations from 66 sites across the Comoros Islands of Mwali, Ngazidja, and Ndzwani revealed that 83.3% of cassava fields had foliar symptoms of CBSD compared with 95.5% for CMD. Molecular diagnostics confirmed the presence of both cassava brown streak ipomoviruses (CBSIs) and cassava mosaic begomoviruses (CMBs). Although real-time RT-PCR only detected the presence of one CBSI species (<i<Cassava brown streak virus</i<, CBSV) the second species (<i<Ugandan cassava brown streak virus</i<, UCBSV) was identified using next-generation high-throughput sequencing. Both PCR and HTS detected the presence of East African cassava mosaic virus (EACMV). African cassava mosaic virus was not detected in any of the samples. Four whitefly species were identified from a sample of 131 specimens: <i<Bemisia tabaci</i<, <i<B. afer</i<, <i<Aleurodicus dispersus</i<, and <i<Paraleyrodes bondari</i<. Cassava <i<B. tabaci</i< comprised two mitotypes: SSA1-SG2 (89%) and SSA1-SG3 (11%). KASP SNP genotyping categorized 82% of cassava <i<B. tabaci</i< as haplogroup SSA-ESA. This knowledge will provide an important base for developing and deploying effective management strategies for cassava viruses and their vectors.
abstractGer	A comprehensive assessment of cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) was carried out in Comoros where cassava yield (5.7 t/ha) is significantly below the African average (8.6 t/ha) largely due to virus diseases. Observations from 66 sites across the Comoros Islands of Mwali, Ngazidja, and Ndzwani revealed that 83.3% of cassava fields had foliar symptoms of CBSD compared with 95.5% for CMD. Molecular diagnostics confirmed the presence of both cassava brown streak ipomoviruses (CBSIs) and cassava mosaic begomoviruses (CMBs). Although real-time RT-PCR only detected the presence of one CBSI species (<i<Cassava brown streak virus</i<, CBSV) the second species (<i<Ugandan cassava brown streak virus</i<, UCBSV) was identified using next-generation high-throughput sequencing. Both PCR and HTS detected the presence of East African cassava mosaic virus (EACMV). African cassava mosaic virus was not detected in any of the samples. Four whitefly species were identified from a sample of 131 specimens: <i<Bemisia tabaci</i<, <i<B. afer</i<, <i<Aleurodicus dispersus</i<, and <i<Paraleyrodes bondari</i<. Cassava <i<B. tabaci</i< comprised two mitotypes: SSA1-SG2 (89%) and SSA1-SG3 (11%). KASP SNP genotyping categorized 82% of cassava <i<B. tabaci</i< as haplogroup SSA-ESA. This knowledge will provide an important base for developing and deploying effective management strategies for cassava viruses and their vectors.
abstract_unstemmed	A comprehensive assessment of cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) was carried out in Comoros where cassava yield (5.7 t/ha) is significantly below the African average (8.6 t/ha) largely due to virus diseases. Observations from 66 sites across the Comoros Islands of Mwali, Ngazidja, and Ndzwani revealed that 83.3% of cassava fields had foliar symptoms of CBSD compared with 95.5% for CMD. Molecular diagnostics confirmed the presence of both cassava brown streak ipomoviruses (CBSIs) and cassava mosaic begomoviruses (CMBs). Although real-time RT-PCR only detected the presence of one CBSI species (<i<Cassava brown streak virus</i<, CBSV) the second species (<i<Ugandan cassava brown streak virus</i<, UCBSV) was identified using next-generation high-throughput sequencing. Both PCR and HTS detected the presence of East African cassava mosaic virus (EACMV). African cassava mosaic virus was not detected in any of the samples. Four whitefly species were identified from a sample of 131 specimens: <i<Bemisia tabaci</i<, <i<B. afer</i<, <i<Aleurodicus dispersus</i<, and <i<Paraleyrodes bondari</i<. Cassava <i<B. tabaci</i< comprised two mitotypes: SSA1-SG2 (89%) and SSA1-SG3 (11%). KASP SNP genotyping categorized 82% of cassava <i<B. tabaci</i< as haplogroup SSA-ESA. This knowledge will provide an important base for developing and deploying effective management strategies for cassava viruses and their vectors.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
container_issue	10, p 2165
title_short	Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and <i<Bemisia tabaci</i< in the Comoros Islands
url	https://doi.org/10.3390/v14102165 https://doaj.org/article/1c88be78da67463eb3fc4d7f07b4e209 https://www.mdpi.com/1999-4915/14/10/2165 https://doaj.org/toc/1999-4915
remote_bool	true
author2	Everlyne Nafula Wosula Abdou Azali Hamza Nobataine Ali Mohammed Hadji Mouigni Salima Nouhou Naima Mmadi Mchinda Gloria Ceasar Massoud Amour Emmanuel Njukwe James Peter Legg
author2Str	Everlyne Nafula Wosula Abdou Azali Hamza Nobataine Ali Mohammed Hadji Mouigni Salima Nouhou Naima Mmadi Mchinda Gloria Ceasar Massoud Amour Emmanuel Njukwe James Peter Legg
ppnlink	609775871
callnumber-subject	QR - Microbiology
mediatype_str_mv	c
isOA_txt	true
hochschulschrift_bool	false
doi_str	10.3390/v14102165
callnumber-a	QR1-502
up_date	2024-07-03T20:04:40.686Z
_version_	1803589601919500288
fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ083904093</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240414173447.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230311s2022 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3390/v14102165</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ083904093</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ1c88be78da67463eb3fc4d7f07b4e209</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QR1-502</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Rudolph Rufini Shirima</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and <i<Bemisia tabaci</i< in the Comoros Islands</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2022</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">A comprehensive assessment of cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) was carried out in Comoros where cassava yield (5.7 t/ha) is significantly below the African average (8.6 t/ha) largely due to virus diseases. Observations from 66 sites across the Comoros Islands of Mwali, Ngazidja, and Ndzwani revealed that 83.3% of cassava fields had foliar symptoms of CBSD compared with 95.5% for CMD. Molecular diagnostics confirmed the presence of both cassava brown streak ipomoviruses (CBSIs) and cassava mosaic begomoviruses (CMBs). Although real-time RT-PCR only detected the presence of one CBSI species (<i<Cassava brown streak virus</i<, CBSV) the second species (<i<Ugandan cassava brown streak virus</i<, UCBSV) was identified using next-generation high-throughput sequencing. Both PCR and HTS detected the presence of East African cassava mosaic virus (EACMV). African cassava mosaic virus was not detected in any of the samples. Four whitefly species were identified from a sample of 131 specimens: <i<Bemisia tabaci</i<, <i<B. afer</i<, <i<Aleurodicus dispersus</i<, and <i<Paraleyrodes bondari</i<. Cassava <i<B. tabaci</i< comprised two mitotypes: SSA1-SG2 (89%) and SSA1-SG3 (11%). KASP SNP genotyping categorized 82% of cassava <i<B. tabaci</i< as haplogroup SSA-ESA. This knowledge will provide an important base for developing and deploying effective management strategies for cassava viruses and their vectors.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">surveillance</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">CBSD</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">CMD</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">CBSIs</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">CMBs</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><i<Bemisia tabaci</i<</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Microbiology</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Everlyne Nafula Wosula</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Abdou Azali Hamza</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Nobataine Ali Mohammed</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Hadji Mouigni</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Salima Nouhou</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Naima Mmadi Mchinda</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Gloria Ceasar</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Massoud Amour</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Emmanuel Njukwe</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">James Peter Legg</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Viruses</subfield><subfield code="d">MDPI AG, 2009</subfield><subfield code="g">14(2022), 10, p 2165</subfield><subfield code="w">(DE-627)609775871</subfield><subfield code="w">(DE-600)2516098-9</subfield><subfield code="x">19994915</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:14</subfield><subfield code="g">year:2022</subfield><subfield code="g">number:10, p 2165</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.3390/v14102165</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/1c88be78da67463eb3fc4d7f07b4e209</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.mdpi.com/1999-4915/14/10/2165</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1999-4915</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_11</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_224</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_370</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">14</subfield><subfield code="j">2022</subfield><subfield code="e">10, p 2165</subfield></datafield></record></collection>
score	7.3993883

Nicht das Richtige dabei?

Schreiben Sie uns!

Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and <i<Bemisia tabaci</i< in the Comoros Islands

Nicht das Richtige dabei?

Zugang & Verfügbarkeit

Vorhandene Bände

Nicht das Richtige dabei?