pH induced stress enhances lipid accumulation in microalgae grown under mixotrophic and autotrophic condition

Production of biodiesel together with wastewater treatment and CO2 sequestration is a promising technology. The growing levels of carbon dioxide in the atmosphere increase the amount of dissolved CO2 in natural watercourses, triggering the increase in concentrations of bicarbonate and hydrogen ions...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Yousef Ahmed Alkhamis [verfasserIn] Roshmon Thomas Mathew [verfasserIn] Ganesan Nagarajan [verfasserIn] Sheikh Mustafizur Rahman [verfasserIn] Md. Moshiur Rahman [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	biodiesel carbohydrates mixed microalgae PH stress water treatment

Übergeordnetes Werk:	In: Frontiers in Energy Research - Frontiers Media S.A., 2014, 10(2022)
Übergeordnetes Werk:	volume:10 ; year:2022

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fenrg.2022.1033068

Katalog-ID:	DOAJ084247959

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topic_title	pH induced stress enhances lipid accumulation in microalgae grown under mixotrophic and autotrophic condition biodiesel carbohydrates mixed microalgae PH stress water treatment
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title	pH induced stress enhances lipid accumulation in microalgae grown under mixotrophic and autotrophic condition
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title_full	pH induced stress enhances lipid accumulation in microalgae grown under mixotrophic and autotrophic condition
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title_sort	ph induced stress enhances lipid accumulation in microalgae grown under mixotrophic and autotrophic condition
title_auth	pH induced stress enhances lipid accumulation in microalgae grown under mixotrophic and autotrophic condition
abstract	Production of biodiesel together with wastewater treatment and CO2 sequestration is a promising technology. The growing levels of carbon dioxide in the atmosphere increase the amount of dissolved CO2 in natural watercourses, triggering the increase in concentrations of bicarbonate and hydrogen ions while dropping those of carbonate and hydroxyl ions. The active carbon cycling in coastal areas, which can result in periodic and daily fluctuations in pH and CO2 concentrations that may surpass those anticipated for the extensive marine ecosystems, is regarded as one of the consequences of climate change. Studies were conducted to examine the effects of various pH levels on algal growth and lipid production in order to better understand how the growth of algae may be influenced in such conditions. In the present study, the influence of three different pH levels (6, 8, and 10) was studied to evaluate microalgae’s carbohydrate utilisation and lipid accumulation during the operation’s starvation phase (SP). Microalgae, in the study, were cultivated in two modes, namely mixotrophic [growth phase (GP)] and autotrophic [pH-induced (SP)] conditions. Enhancement in biomass formation, and intracellular carbohydrate accumulation were recorded during the GP operation, while noticeable lipid productivities (Total/neutral, 26.93/10.3%) were observed during SP operation at pH 8. Pigment analysis showed variations in both the procedures where higher Chl a concentration was noticed in GP, and higher Chl b was detected during SP. Nile red fluorescent staining strongly supports the existence of intracellular lipid bodies (LB). GC analysis of fatty acid methyl esters (FAME) showed the existence of a substantial amount of saturated fatty acids (SFA) compared with unsaturated fatty acids (USFA). Efficient wastewater treatment with nutrient assimilation was reported during the GP operation, demonstrating the phyco-remediation.
abstractGer	Production of biodiesel together with wastewater treatment and CO2 sequestration is a promising technology. The growing levels of carbon dioxide in the atmosphere increase the amount of dissolved CO2 in natural watercourses, triggering the increase in concentrations of bicarbonate and hydrogen ions while dropping those of carbonate and hydroxyl ions. The active carbon cycling in coastal areas, which can result in periodic and daily fluctuations in pH and CO2 concentrations that may surpass those anticipated for the extensive marine ecosystems, is regarded as one of the consequences of climate change. Studies were conducted to examine the effects of various pH levels on algal growth and lipid production in order to better understand how the growth of algae may be influenced in such conditions. In the present study, the influence of three different pH levels (6, 8, and 10) was studied to evaluate microalgae’s carbohydrate utilisation and lipid accumulation during the operation’s starvation phase (SP). Microalgae, in the study, were cultivated in two modes, namely mixotrophic [growth phase (GP)] and autotrophic [pH-induced (SP)] conditions. Enhancement in biomass formation, and intracellular carbohydrate accumulation were recorded during the GP operation, while noticeable lipid productivities (Total/neutral, 26.93/10.3%) were observed during SP operation at pH 8. Pigment analysis showed variations in both the procedures where higher Chl a concentration was noticed in GP, and higher Chl b was detected during SP. Nile red fluorescent staining strongly supports the existence of intracellular lipid bodies (LB). GC analysis of fatty acid methyl esters (FAME) showed the existence of a substantial amount of saturated fatty acids (SFA) compared with unsaturated fatty acids (USFA). Efficient wastewater treatment with nutrient assimilation was reported during the GP operation, demonstrating the phyco-remediation.
abstract_unstemmed	Production of biodiesel together with wastewater treatment and CO2 sequestration is a promising technology. The growing levels of carbon dioxide in the atmosphere increase the amount of dissolved CO2 in natural watercourses, triggering the increase in concentrations of bicarbonate and hydrogen ions while dropping those of carbonate and hydroxyl ions. The active carbon cycling in coastal areas, which can result in periodic and daily fluctuations in pH and CO2 concentrations that may surpass those anticipated for the extensive marine ecosystems, is regarded as one of the consequences of climate change. Studies were conducted to examine the effects of various pH levels on algal growth and lipid production in order to better understand how the growth of algae may be influenced in such conditions. In the present study, the influence of three different pH levels (6, 8, and 10) was studied to evaluate microalgae’s carbohydrate utilisation and lipid accumulation during the operation’s starvation phase (SP). Microalgae, in the study, were cultivated in two modes, namely mixotrophic [growth phase (GP)] and autotrophic [pH-induced (SP)] conditions. Enhancement in biomass formation, and intracellular carbohydrate accumulation were recorded during the GP operation, while noticeable lipid productivities (Total/neutral, 26.93/10.3%) were observed during SP operation at pH 8. Pigment analysis showed variations in both the procedures where higher Chl a concentration was noticed in GP, and higher Chl b was detected during SP. Nile red fluorescent staining strongly supports the existence of intracellular lipid bodies (LB). GC analysis of fatty acid methyl esters (FAME) showed the existence of a substantial amount of saturated fatty acids (SFA) compared with unsaturated fatty acids (USFA). Efficient wastewater treatment with nutrient assimilation was reported during the GP operation, demonstrating the phyco-remediation.
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title_short	pH induced stress enhances lipid accumulation in microalgae grown under mixotrophic and autotrophic condition
url	https://doi.org/10.3389/fenrg.2022.1033068 https://doaj.org/article/d9b21671b82245d4b628ca665c2188ff https://www.frontiersin.org/articles/10.3389/fenrg.2022.1033068/full https://doaj.org/toc/2296-598X
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The growing levels of carbon dioxide in the atmosphere increase the amount of dissolved CO2 in natural watercourses, triggering the increase in concentrations of bicarbonate and hydrogen ions while dropping those of carbonate and hydroxyl ions. The active carbon cycling in coastal areas, which can result in periodic and daily fluctuations in pH and CO2 concentrations that may surpass those anticipated for the extensive marine ecosystems, is regarded as one of the consequences of climate change. Studies were conducted to examine the effects of various pH levels on algal growth and lipid production in order to better understand how the growth of algae may be influenced in such conditions. In the present study, the influence of three different pH levels (6, 8, and 10) was studied to evaluate microalgae’s carbohydrate utilisation and lipid accumulation during the operation’s starvation phase (SP). Microalgae, in the study, were cultivated in two modes, namely mixotrophic [growth phase (GP)] and autotrophic [pH-induced (SP)] conditions. Enhancement in biomass formation, and intracellular carbohydrate accumulation were recorded during the GP operation, while noticeable lipid productivities (Total/neutral, 26.93/10.3%) were observed during SP operation at pH 8. Pigment analysis showed variations in both the procedures where higher Chl a concentration was noticed in GP, and higher Chl b was detected during SP. Nile red fluorescent staining strongly supports the existence of intracellular lipid bodies (LB). GC analysis of fatty acid methyl esters (FAME) showed the existence of a substantial amount of saturated fatty acids (SFA) compared with unsaturated fatty acids (USFA). Efficient wastewater treatment with nutrient assimilation was reported during the GP operation, demonstrating the phyco-remediation.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">biodiesel</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">carbohydrates</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">mixed microalgae</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">PH stress</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">water treatment</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">General Works</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">A</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Yousef Ahmed Alkhamis</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Roshmon Thomas Mathew</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Ganesan Nagarajan</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Ganesan Nagarajan</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Ganesan Nagarajan</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Sheikh Mustafizur Rahman</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Md. 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pH induced stress enhances lipid accumulation in microalgae grown under mixotrophic and autotrophic condition

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