Differential induction of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by Trichophyton rubrum and Microsporum gypseum
Objective: To explore the activation of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by different ecological classifications of dermatophytes. Methods: Total and phosphorylated p65, relb, IκB, and c-Jun in HaCaT cells were detected by immunoblotting following co-culture wit...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Zhen SU [verfasserIn] Weiwei DENG [verfasserIn] Shuting ZHAN [verfasserIn] Panpan LIANG [verfasserIn] Meirong LI [verfasserIn] Songchao YIN [verfasserIn] Jian CHEN [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Chinesisch |
| Erschienen: |
2021 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Pifu-xingbing zhenliaoxue zazhi - editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology, 2022, 28(2021), 1, Seite 6 |
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| Übergeordnetes Werk: |
volume:28 ; year:2021 ; number:1 ; pages:6 |
| Links: |
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| DOI / URN: |
10.3969/j.issn.1674-8468.2021.01.001 |
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| Katalog-ID: |
DOAJ084481358 |
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| 520 | |a Objective: To explore the activation of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by different ecological classifications of dermatophytes. Methods: Total and phosphorylated p65, relb, IκB, and c-Jun in HaCaT cells were detected by immunoblotting following co-culture with either Trichophyton rubrum(T. rubrum) or Microsporum gypseum(M. gypseum) for 24 h. Immunoblotting was used to assess expression levels of TLR2, c-Jun, and phosphorylated c-Jun after co-culture of TLR2 deficient HaCaT cells (HaCaT TLR2-/- cells) with T. rubrum and M. gypseum for 24 h. Results: Co-culture of HaCaT cells with M. gypseum, but not T. rubrum, significantly increased the levels of phosphorylated c-Jun. However, neither T. rubrum nor M. gypseum induced significant changes in the total and phosphorylated p56, relb, and IκB in HaCaT cells. Following TLR2 knockout in HaCaT cells, the level of phosphorylated c-Jun decreased significantly in HaCaT TLR2-/- cells co-cultured with T. rubrum, but not M. gypseum. Conclusions: T. rubrum and M. gypseum can activate c-Jun signaling pathway through TLR2 in keratinocytes, without activation of NF-κB signaling pathway. TLR2, together with other receptors, may dominate the differential induction of c-Jun pathway in keratinocytes infected by T. rubrum and M. gypseum. | ||
| 650 | 4 | |a keratinocytes | |
| 650 | 4 | |a trichophyton rubrum | |
| 650 | 4 | |a microsporum gypseum | |
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10.3969/j.issn.1674-8468.2021.01.001 doi (DE-627)DOAJ084481358 (DE-599)DOAJddd6d7bf240e439fb41270c055878ad0 DE-627 ger DE-627 rakwb chi RL1-803 Zhen SU verfasserin aut Differential induction of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by Trichophyton rubrum and Microsporum gypseum 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: To explore the activation of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by different ecological classifications of dermatophytes. Methods: Total and phosphorylated p65, relb, IκB, and c-Jun in HaCaT cells were detected by immunoblotting following co-culture with either Trichophyton rubrum(T. rubrum) or Microsporum gypseum(M. gypseum) for 24 h. Immunoblotting was used to assess expression levels of TLR2, c-Jun, and phosphorylated c-Jun after co-culture of TLR2 deficient HaCaT cells (HaCaT TLR2-/- cells) with T. rubrum and M. gypseum for 24 h. Results: Co-culture of HaCaT cells with M. gypseum, but not T. rubrum, significantly increased the levels of phosphorylated c-Jun. However, neither T. rubrum nor M. gypseum induced significant changes in the total and phosphorylated p56, relb, and IκB in HaCaT cells. Following TLR2 knockout in HaCaT cells, the level of phosphorylated c-Jun decreased significantly in HaCaT TLR2-/- cells co-cultured with T. rubrum, but not M. gypseum. Conclusions: T. rubrum and M. gypseum can activate c-Jun signaling pathway through TLR2 in keratinocytes, without activation of NF-κB signaling pathway. TLR2, together with other receptors, may dominate the differential induction of c-Jun pathway in keratinocytes infected by T. rubrum and M. gypseum. keratinocytes trichophyton rubrum microsporum gypseum tlr2 c-jun signaling pathway Dermatology Weiwei DENG verfasserin aut Shuting ZHAN verfasserin aut Panpan LIANG verfasserin aut Meirong LI verfasserin aut Songchao YIN verfasserin aut Jian CHEN verfasserin aut In Pifu-xingbing zhenliaoxue zazhi editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology, 2022 28(2021), 1, Seite 6 (DE-627)DOAJ078597897 16748468 nnns volume:28 year:2021 number:1 pages:6 https://doi.org/10.3969/j.issn.1674-8468.2021.01.001 kostenfrei https://doaj.org/article/ddd6d7bf240e439fb41270c055878ad0 kostenfrei http://pfxbzlx.gdvdc.com/CN/abstract/abstract1900.shtml kostenfrei https://doaj.org/toc/1674-8468 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 28 2021 1 6 |
| spelling |
10.3969/j.issn.1674-8468.2021.01.001 doi (DE-627)DOAJ084481358 (DE-599)DOAJddd6d7bf240e439fb41270c055878ad0 DE-627 ger DE-627 rakwb chi RL1-803 Zhen SU verfasserin aut Differential induction of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by Trichophyton rubrum and Microsporum gypseum 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: To explore the activation of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by different ecological classifications of dermatophytes. Methods: Total and phosphorylated p65, relb, IκB, and c-Jun in HaCaT cells were detected by immunoblotting following co-culture with either Trichophyton rubrum(T. rubrum) or Microsporum gypseum(M. gypseum) for 24 h. Immunoblotting was used to assess expression levels of TLR2, c-Jun, and phosphorylated c-Jun after co-culture of TLR2 deficient HaCaT cells (HaCaT TLR2-/- cells) with T. rubrum and M. gypseum for 24 h. Results: Co-culture of HaCaT cells with M. gypseum, but not T. rubrum, significantly increased the levels of phosphorylated c-Jun. However, neither T. rubrum nor M. gypseum induced significant changes in the total and phosphorylated p56, relb, and IκB in HaCaT cells. Following TLR2 knockout in HaCaT cells, the level of phosphorylated c-Jun decreased significantly in HaCaT TLR2-/- cells co-cultured with T. rubrum, but not M. gypseum. Conclusions: T. rubrum and M. gypseum can activate c-Jun signaling pathway through TLR2 in keratinocytes, without activation of NF-κB signaling pathway. TLR2, together with other receptors, may dominate the differential induction of c-Jun pathway in keratinocytes infected by T. rubrum and M. gypseum. keratinocytes trichophyton rubrum microsporum gypseum tlr2 c-jun signaling pathway Dermatology Weiwei DENG verfasserin aut Shuting ZHAN verfasserin aut Panpan LIANG verfasserin aut Meirong LI verfasserin aut Songchao YIN verfasserin aut Jian CHEN verfasserin aut In Pifu-xingbing zhenliaoxue zazhi editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology, 2022 28(2021), 1, Seite 6 (DE-627)DOAJ078597897 16748468 nnns volume:28 year:2021 number:1 pages:6 https://doi.org/10.3969/j.issn.1674-8468.2021.01.001 kostenfrei https://doaj.org/article/ddd6d7bf240e439fb41270c055878ad0 kostenfrei http://pfxbzlx.gdvdc.com/CN/abstract/abstract1900.shtml kostenfrei https://doaj.org/toc/1674-8468 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 28 2021 1 6 |
| allfields_unstemmed |
10.3969/j.issn.1674-8468.2021.01.001 doi (DE-627)DOAJ084481358 (DE-599)DOAJddd6d7bf240e439fb41270c055878ad0 DE-627 ger DE-627 rakwb chi RL1-803 Zhen SU verfasserin aut Differential induction of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by Trichophyton rubrum and Microsporum gypseum 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: To explore the activation of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by different ecological classifications of dermatophytes. Methods: Total and phosphorylated p65, relb, IκB, and c-Jun in HaCaT cells were detected by immunoblotting following co-culture with either Trichophyton rubrum(T. rubrum) or Microsporum gypseum(M. gypseum) for 24 h. Immunoblotting was used to assess expression levels of TLR2, c-Jun, and phosphorylated c-Jun after co-culture of TLR2 deficient HaCaT cells (HaCaT TLR2-/- cells) with T. rubrum and M. gypseum for 24 h. Results: Co-culture of HaCaT cells with M. gypseum, but not T. rubrum, significantly increased the levels of phosphorylated c-Jun. However, neither T. rubrum nor M. gypseum induced significant changes in the total and phosphorylated p56, relb, and IκB in HaCaT cells. Following TLR2 knockout in HaCaT cells, the level of phosphorylated c-Jun decreased significantly in HaCaT TLR2-/- cells co-cultured with T. rubrum, but not M. gypseum. Conclusions: T. rubrum and M. gypseum can activate c-Jun signaling pathway through TLR2 in keratinocytes, without activation of NF-κB signaling pathway. TLR2, together with other receptors, may dominate the differential induction of c-Jun pathway in keratinocytes infected by T. rubrum and M. gypseum. keratinocytes trichophyton rubrum microsporum gypseum tlr2 c-jun signaling pathway Dermatology Weiwei DENG verfasserin aut Shuting ZHAN verfasserin aut Panpan LIANG verfasserin aut Meirong LI verfasserin aut Songchao YIN verfasserin aut Jian CHEN verfasserin aut In Pifu-xingbing zhenliaoxue zazhi editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology, 2022 28(2021), 1, Seite 6 (DE-627)DOAJ078597897 16748468 nnns volume:28 year:2021 number:1 pages:6 https://doi.org/10.3969/j.issn.1674-8468.2021.01.001 kostenfrei https://doaj.org/article/ddd6d7bf240e439fb41270c055878ad0 kostenfrei http://pfxbzlx.gdvdc.com/CN/abstract/abstract1900.shtml kostenfrei https://doaj.org/toc/1674-8468 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 28 2021 1 6 |
| allfieldsGer |
10.3969/j.issn.1674-8468.2021.01.001 doi (DE-627)DOAJ084481358 (DE-599)DOAJddd6d7bf240e439fb41270c055878ad0 DE-627 ger DE-627 rakwb chi RL1-803 Zhen SU verfasserin aut Differential induction of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by Trichophyton rubrum and Microsporum gypseum 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: To explore the activation of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by different ecological classifications of dermatophytes. Methods: Total and phosphorylated p65, relb, IκB, and c-Jun in HaCaT cells were detected by immunoblotting following co-culture with either Trichophyton rubrum(T. rubrum) or Microsporum gypseum(M. gypseum) for 24 h. Immunoblotting was used to assess expression levels of TLR2, c-Jun, and phosphorylated c-Jun after co-culture of TLR2 deficient HaCaT cells (HaCaT TLR2-/- cells) with T. rubrum and M. gypseum for 24 h. Results: Co-culture of HaCaT cells with M. gypseum, but not T. rubrum, significantly increased the levels of phosphorylated c-Jun. However, neither T. rubrum nor M. gypseum induced significant changes in the total and phosphorylated p56, relb, and IκB in HaCaT cells. Following TLR2 knockout in HaCaT cells, the level of phosphorylated c-Jun decreased significantly in HaCaT TLR2-/- cells co-cultured with T. rubrum, but not M. gypseum. Conclusions: T. rubrum and M. gypseum can activate c-Jun signaling pathway through TLR2 in keratinocytes, without activation of NF-κB signaling pathway. TLR2, together with other receptors, may dominate the differential induction of c-Jun pathway in keratinocytes infected by T. rubrum and M. gypseum. keratinocytes trichophyton rubrum microsporum gypseum tlr2 c-jun signaling pathway Dermatology Weiwei DENG verfasserin aut Shuting ZHAN verfasserin aut Panpan LIANG verfasserin aut Meirong LI verfasserin aut Songchao YIN verfasserin aut Jian CHEN verfasserin aut In Pifu-xingbing zhenliaoxue zazhi editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology, 2022 28(2021), 1, Seite 6 (DE-627)DOAJ078597897 16748468 nnns volume:28 year:2021 number:1 pages:6 https://doi.org/10.3969/j.issn.1674-8468.2021.01.001 kostenfrei https://doaj.org/article/ddd6d7bf240e439fb41270c055878ad0 kostenfrei http://pfxbzlx.gdvdc.com/CN/abstract/abstract1900.shtml kostenfrei https://doaj.org/toc/1674-8468 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 28 2021 1 6 |
| allfieldsSound |
10.3969/j.issn.1674-8468.2021.01.001 doi (DE-627)DOAJ084481358 (DE-599)DOAJddd6d7bf240e439fb41270c055878ad0 DE-627 ger DE-627 rakwb chi RL1-803 Zhen SU verfasserin aut Differential induction of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by Trichophyton rubrum and Microsporum gypseum 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: To explore the activation of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by different ecological classifications of dermatophytes. Methods: Total and phosphorylated p65, relb, IκB, and c-Jun in HaCaT cells were detected by immunoblotting following co-culture with either Trichophyton rubrum(T. rubrum) or Microsporum gypseum(M. gypseum) for 24 h. Immunoblotting was used to assess expression levels of TLR2, c-Jun, and phosphorylated c-Jun after co-culture of TLR2 deficient HaCaT cells (HaCaT TLR2-/- cells) with T. rubrum and M. gypseum for 24 h. Results: Co-culture of HaCaT cells with M. gypseum, but not T. rubrum, significantly increased the levels of phosphorylated c-Jun. However, neither T. rubrum nor M. gypseum induced significant changes in the total and phosphorylated p56, relb, and IκB in HaCaT cells. Following TLR2 knockout in HaCaT cells, the level of phosphorylated c-Jun decreased significantly in HaCaT TLR2-/- cells co-cultured with T. rubrum, but not M. gypseum. Conclusions: T. rubrum and M. gypseum can activate c-Jun signaling pathway through TLR2 in keratinocytes, without activation of NF-κB signaling pathway. TLR2, together with other receptors, may dominate the differential induction of c-Jun pathway in keratinocytes infected by T. rubrum and M. gypseum. keratinocytes trichophyton rubrum microsporum gypseum tlr2 c-jun signaling pathway Dermatology Weiwei DENG verfasserin aut Shuting ZHAN verfasserin aut Panpan LIANG verfasserin aut Meirong LI verfasserin aut Songchao YIN verfasserin aut Jian CHEN verfasserin aut In Pifu-xingbing zhenliaoxue zazhi editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology, 2022 28(2021), 1, Seite 6 (DE-627)DOAJ078597897 16748468 nnns volume:28 year:2021 number:1 pages:6 https://doi.org/10.3969/j.issn.1674-8468.2021.01.001 kostenfrei https://doaj.org/article/ddd6d7bf240e439fb41270c055878ad0 kostenfrei http://pfxbzlx.gdvdc.com/CN/abstract/abstract1900.shtml kostenfrei https://doaj.org/toc/1674-8468 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 28 2021 1 6 |
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In Pifu-xingbing zhenliaoxue zazhi 28(2021), 1, Seite 6 volume:28 year:2021 number:1 pages:6 |
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Methods: Total and phosphorylated p65, relb, IκB, and c-Jun in HaCaT cells were detected by immunoblotting following co-culture with either Trichophyton rubrum(T. rubrum) or Microsporum gypseum(M. gypseum) for 24 h. Immunoblotting was used to assess expression levels of TLR2, c-Jun, and phosphorylated c-Jun after co-culture of TLR2 deficient HaCaT cells (HaCaT TLR2-/- cells) with T. rubrum and M. gypseum for 24 h. Results: Co-culture of HaCaT cells with M. gypseum, but not T. rubrum, significantly increased the levels of phosphorylated c-Jun. However, neither T. rubrum nor M. gypseum induced significant changes in the total and phosphorylated p56, relb, and IκB in HaCaT cells. Following TLR2 knockout in HaCaT cells, the level of phosphorylated c-Jun decreased significantly in HaCaT TLR2-/- cells co-cultured with T. rubrum, but not M. gypseum. Conclusions: T. rubrum and M. gypseum can activate c-Jun signaling pathway through TLR2 in keratinocytes, without activation of NF-κB signaling pathway. 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RL1-803 Differential induction of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by Trichophyton rubrum and Microsporum gypseum keratinocytes trichophyton rubrum microsporum gypseum tlr2 c-jun signaling pathway |
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Differential induction of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by Trichophyton rubrum and Microsporum gypseum |
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Differential induction of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by Trichophyton rubrum and Microsporum gypseum |
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Zhen SU Weiwei DENG Shuting ZHAN Panpan LIANG Meirong LI Songchao YIN Jian CHEN |
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differential induction of tlr2/c-jun and nf-κb signaling pathway in human keratinocytes infected by trichophyton rubrum and microsporum gypseum |
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Differential induction of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by Trichophyton rubrum and Microsporum gypseum |
| abstract |
Objective: To explore the activation of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by different ecological classifications of dermatophytes. Methods: Total and phosphorylated p65, relb, IκB, and c-Jun in HaCaT cells were detected by immunoblotting following co-culture with either Trichophyton rubrum(T. rubrum) or Microsporum gypseum(M. gypseum) for 24 h. Immunoblotting was used to assess expression levels of TLR2, c-Jun, and phosphorylated c-Jun after co-culture of TLR2 deficient HaCaT cells (HaCaT TLR2-/- cells) with T. rubrum and M. gypseum for 24 h. Results: Co-culture of HaCaT cells with M. gypseum, but not T. rubrum, significantly increased the levels of phosphorylated c-Jun. However, neither T. rubrum nor M. gypseum induced significant changes in the total and phosphorylated p56, relb, and IκB in HaCaT cells. Following TLR2 knockout in HaCaT cells, the level of phosphorylated c-Jun decreased significantly in HaCaT TLR2-/- cells co-cultured with T. rubrum, but not M. gypseum. Conclusions: T. rubrum and M. gypseum can activate c-Jun signaling pathway through TLR2 in keratinocytes, without activation of NF-κB signaling pathway. TLR2, together with other receptors, may dominate the differential induction of c-Jun pathway in keratinocytes infected by T. rubrum and M. gypseum. |
| abstractGer |
Objective: To explore the activation of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by different ecological classifications of dermatophytes. Methods: Total and phosphorylated p65, relb, IκB, and c-Jun in HaCaT cells were detected by immunoblotting following co-culture with either Trichophyton rubrum(T. rubrum) or Microsporum gypseum(M. gypseum) for 24 h. Immunoblotting was used to assess expression levels of TLR2, c-Jun, and phosphorylated c-Jun after co-culture of TLR2 deficient HaCaT cells (HaCaT TLR2-/- cells) with T. rubrum and M. gypseum for 24 h. Results: Co-culture of HaCaT cells with M. gypseum, but not T. rubrum, significantly increased the levels of phosphorylated c-Jun. However, neither T. rubrum nor M. gypseum induced significant changes in the total and phosphorylated p56, relb, and IκB in HaCaT cells. Following TLR2 knockout in HaCaT cells, the level of phosphorylated c-Jun decreased significantly in HaCaT TLR2-/- cells co-cultured with T. rubrum, but not M. gypseum. Conclusions: T. rubrum and M. gypseum can activate c-Jun signaling pathway through TLR2 in keratinocytes, without activation of NF-κB signaling pathway. TLR2, together with other receptors, may dominate the differential induction of c-Jun pathway in keratinocytes infected by T. rubrum and M. gypseum. |
| abstract_unstemmed |
Objective: To explore the activation of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by different ecological classifications of dermatophytes. Methods: Total and phosphorylated p65, relb, IκB, and c-Jun in HaCaT cells were detected by immunoblotting following co-culture with either Trichophyton rubrum(T. rubrum) or Microsporum gypseum(M. gypseum) for 24 h. Immunoblotting was used to assess expression levels of TLR2, c-Jun, and phosphorylated c-Jun after co-culture of TLR2 deficient HaCaT cells (HaCaT TLR2-/- cells) with T. rubrum and M. gypseum for 24 h. Results: Co-culture of HaCaT cells with M. gypseum, but not T. rubrum, significantly increased the levels of phosphorylated c-Jun. However, neither T. rubrum nor M. gypseum induced significant changes in the total and phosphorylated p56, relb, and IκB in HaCaT cells. Following TLR2 knockout in HaCaT cells, the level of phosphorylated c-Jun decreased significantly in HaCaT TLR2-/- cells co-cultured with T. rubrum, but not M. gypseum. Conclusions: T. rubrum and M. gypseum can activate c-Jun signaling pathway through TLR2 in keratinocytes, without activation of NF-κB signaling pathway. TLR2, together with other receptors, may dominate the differential induction of c-Jun pathway in keratinocytes infected by T. rubrum and M. gypseum. |
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Differential induction of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by Trichophyton rubrum and Microsporum gypseum |
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