Phenotypic Differentiation of Two Morphologically Similar Aflatoxin-Producing Fungi from West Africa
Aflatoxins (AF) are hepatocarcinogenic metabolites produced by several <i<Aspergillus</i< species. Crop infection by these species results in aflatoxin contamination of cereals, nuts, and spices. Etiology of aflatoxin contamination is complicated by mixed infections of multiple species w...
Ausführliche Beschreibung
Autor*in: |
Pummi Singh [verfasserIn] Hillary L. Mehl [verfasserIn] Marc J. Orbach [verfasserIn] Kenneth A. Callicott [verfasserIn] Peter J. Cotty [verfasserIn] |
---|
Format: |
E-Artikel |
---|---|
Sprache: |
Englisch |
Erschienen: |
2020 |
---|
Schlagwörter: |
---|
Übergeordnetes Werk: |
In: Toxins - MDPI AG, 2010, 12(2020), 10, p 656 |
---|---|
Übergeordnetes Werk: |
volume:12 ; year:2020 ; number:10, p 656 |
Links: |
---|
DOI / URN: |
10.3390/toxins12100656 |
---|
Katalog-ID: |
DOAJ084652101 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | DOAJ084652101 | ||
003 | DE-627 | ||
005 | 20240412212149.0 | ||
007 | cr uuu---uuuuu | ||
008 | 230311s2020 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.3390/toxins12100656 |2 doi | |
035 | |a (DE-627)DOAJ084652101 | ||
035 | |a (DE-599)DOAJ45f628ff4c17463eb47be85d11325442 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 0 | |a Pummi Singh |e verfasserin |4 aut | |
245 | 1 | 0 | |a Phenotypic Differentiation of Two Morphologically Similar Aflatoxin-Producing Fungi from West Africa |
264 | 1 | |c 2020 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a Computermedien |b c |2 rdamedia | ||
338 | |a Online-Ressource |b cr |2 rdacarrier | ||
520 | |a Aflatoxins (AF) are hepatocarcinogenic metabolites produced by several <i<Aspergillus</i< species. Crop infection by these species results in aflatoxin contamination of cereals, nuts, and spices. Etiology of aflatoxin contamination is complicated by mixed infections of multiple species with similar morphology and aflatoxin profiles. The current study investigates variation in aflatoxin production between two morphologically similar species that co-exist in West Africa, <i<A. aflatoxiformans</i< and <i<A. minisclerotigenes</i<. Consistent distinctions in aflatoxin production during liquid fermentation were discovered between these species. The two species produced similar concentrations of AFB<sub<1</sub< in defined media with either urea or ammonium as the sole nitrogen source. However, production of both AFB<sub<1</sub< and AFG<sub<1</sub< were inhibited (<i<p</i< < 0.001) for <i<A. aflatoxiformans</i< in a yeast extract medium with sucrose. Although production of AFG<sub<1</sub< by both species was similar in urea, <i<A. minisclerotigenes</i< produced greater concentrations of AFG<sub<1</sub< in ammonium (<i<p</i< = 0.039). Based on these differences, a reliable and convenient assay for differentiating the two species was designed. This assay will be useful for identifying specific etiologic agents of aflatoxin contamination episodes in West Africa and other regions where the two species are sympatric, especially when phylogenetic analyses based on multiple gene segments are not practical. | ||
650 | 4 | |a aflatoxins | |
650 | 4 | |a Aspergillus | |
650 | 4 | |a <i<Aspergillus aflatoxiformans</i< | |
650 | 4 | |a <i<Aspergillus minisclerotigenes</i< | |
653 | 0 | |a Medicine | |
653 | 0 | |a R | |
700 | 0 | |a Hillary L. Mehl |e verfasserin |4 aut | |
700 | 0 | |a Marc J. Orbach |e verfasserin |4 aut | |
700 | 0 | |a Kenneth A. Callicott |e verfasserin |4 aut | |
700 | 0 | |a Peter J. Cotty |e verfasserin |4 aut | |
773 | 0 | 8 | |i In |t Toxins |d MDPI AG, 2010 |g 12(2020), 10, p 656 |w (DE-627)610604236 |w (DE-600)2518395-3 |x 20726651 |7 nnns |
773 | 1 | 8 | |g volume:12 |g year:2020 |g number:10, p 656 |
856 | 4 | 0 | |u https://doi.org/10.3390/toxins12100656 |z kostenfrei |
856 | 4 | 0 | |u https://doaj.org/article/45f628ff4c17463eb47be85d11325442 |z kostenfrei |
856 | 4 | 0 | |u https://www.mdpi.com/2072-6651/12/10/656 |z kostenfrei |
856 | 4 | 2 | |u https://doaj.org/toc/2072-6651 |y Journal toc |z kostenfrei |
912 | |a GBV_USEFLAG_A | ||
912 | |a SYSFLAG_A | ||
912 | |a GBV_DOAJ | ||
912 | |a GBV_ILN_20 | ||
912 | |a GBV_ILN_22 | ||
912 | |a GBV_ILN_23 | ||
912 | |a GBV_ILN_24 | ||
912 | |a GBV_ILN_31 | ||
912 | |a GBV_ILN_39 | ||
912 | |a GBV_ILN_40 | ||
912 | |a GBV_ILN_60 | ||
912 | |a GBV_ILN_62 | ||
912 | |a GBV_ILN_63 | ||
912 | |a GBV_ILN_65 | ||
912 | |a GBV_ILN_69 | ||
912 | |a GBV_ILN_73 | ||
912 | |a GBV_ILN_74 | ||
912 | |a GBV_ILN_95 | ||
912 | |a GBV_ILN_105 | ||
912 | |a GBV_ILN_110 | ||
912 | |a GBV_ILN_151 | ||
912 | |a GBV_ILN_161 | ||
912 | |a GBV_ILN_170 | ||
912 | |a GBV_ILN_206 | ||
912 | |a GBV_ILN_213 | ||
912 | |a GBV_ILN_224 | ||
912 | |a GBV_ILN_230 | ||
912 | |a GBV_ILN_285 | ||
912 | |a GBV_ILN_293 | ||
912 | |a GBV_ILN_602 | ||
912 | |a GBV_ILN_2005 | ||
912 | |a GBV_ILN_2009 | ||
912 | |a GBV_ILN_2011 | ||
912 | |a GBV_ILN_2014 | ||
912 | |a GBV_ILN_2055 | ||
912 | |a GBV_ILN_2111 | ||
912 | |a GBV_ILN_4012 | ||
912 | |a GBV_ILN_4037 | ||
912 | |a GBV_ILN_4112 | ||
912 | |a GBV_ILN_4125 | ||
912 | |a GBV_ILN_4126 | ||
912 | |a GBV_ILN_4249 | ||
912 | |a GBV_ILN_4305 | ||
912 | |a GBV_ILN_4306 | ||
912 | |a GBV_ILN_4307 | ||
912 | |a GBV_ILN_4313 | ||
912 | |a GBV_ILN_4322 | ||
912 | |a GBV_ILN_4323 | ||
912 | |a GBV_ILN_4324 | ||
912 | |a GBV_ILN_4325 | ||
912 | |a GBV_ILN_4338 | ||
912 | |a GBV_ILN_4367 | ||
912 | |a GBV_ILN_4700 | ||
951 | |a AR | ||
952 | |d 12 |j 2020 |e 10, p 656 |
author_variant |
p s ps h l m hlm m j o mjo k a c kac p j c pjc |
---|---|
matchkey_str |
article:20726651:2020----::hntpcifrnitootoopooialsmlrfaoipou |
hierarchy_sort_str |
2020 |
publishDate |
2020 |
allfields |
10.3390/toxins12100656 doi (DE-627)DOAJ084652101 (DE-599)DOAJ45f628ff4c17463eb47be85d11325442 DE-627 ger DE-627 rakwb eng Pummi Singh verfasserin aut Phenotypic Differentiation of Two Morphologically Similar Aflatoxin-Producing Fungi from West Africa 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aflatoxins (AF) are hepatocarcinogenic metabolites produced by several <i<Aspergillus</i< species. Crop infection by these species results in aflatoxin contamination of cereals, nuts, and spices. Etiology of aflatoxin contamination is complicated by mixed infections of multiple species with similar morphology and aflatoxin profiles. The current study investigates variation in aflatoxin production between two morphologically similar species that co-exist in West Africa, <i<A. aflatoxiformans</i< and <i<A. minisclerotigenes</i<. Consistent distinctions in aflatoxin production during liquid fermentation were discovered between these species. The two species produced similar concentrations of AFB<sub<1</sub< in defined media with either urea or ammonium as the sole nitrogen source. However, production of both AFB<sub<1</sub< and AFG<sub<1</sub< were inhibited (<i<p</i< < 0.001) for <i<A. aflatoxiformans</i< in a yeast extract medium with sucrose. Although production of AFG<sub<1</sub< by both species was similar in urea, <i<A. minisclerotigenes</i< produced greater concentrations of AFG<sub<1</sub< in ammonium (<i<p</i< = 0.039). Based on these differences, a reliable and convenient assay for differentiating the two species was designed. This assay will be useful for identifying specific etiologic agents of aflatoxin contamination episodes in West Africa and other regions where the two species are sympatric, especially when phylogenetic analyses based on multiple gene segments are not practical. aflatoxins Aspergillus <i<Aspergillus aflatoxiformans</i< <i<Aspergillus minisclerotigenes</i< Medicine R Hillary L. Mehl verfasserin aut Marc J. Orbach verfasserin aut Kenneth A. Callicott verfasserin aut Peter J. Cotty verfasserin aut In Toxins MDPI AG, 2010 12(2020), 10, p 656 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:12 year:2020 number:10, p 656 https://doi.org/10.3390/toxins12100656 kostenfrei https://doaj.org/article/45f628ff4c17463eb47be85d11325442 kostenfrei https://www.mdpi.com/2072-6651/12/10/656 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 10, p 656 |
spelling |
10.3390/toxins12100656 doi (DE-627)DOAJ084652101 (DE-599)DOAJ45f628ff4c17463eb47be85d11325442 DE-627 ger DE-627 rakwb eng Pummi Singh verfasserin aut Phenotypic Differentiation of Two Morphologically Similar Aflatoxin-Producing Fungi from West Africa 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aflatoxins (AF) are hepatocarcinogenic metabolites produced by several <i<Aspergillus</i< species. Crop infection by these species results in aflatoxin contamination of cereals, nuts, and spices. Etiology of aflatoxin contamination is complicated by mixed infections of multiple species with similar morphology and aflatoxin profiles. The current study investigates variation in aflatoxin production between two morphologically similar species that co-exist in West Africa, <i<A. aflatoxiformans</i< and <i<A. minisclerotigenes</i<. Consistent distinctions in aflatoxin production during liquid fermentation were discovered between these species. The two species produced similar concentrations of AFB<sub<1</sub< in defined media with either urea or ammonium as the sole nitrogen source. However, production of both AFB<sub<1</sub< and AFG<sub<1</sub< were inhibited (<i<p</i< < 0.001) for <i<A. aflatoxiformans</i< in a yeast extract medium with sucrose. Although production of AFG<sub<1</sub< by both species was similar in urea, <i<A. minisclerotigenes</i< produced greater concentrations of AFG<sub<1</sub< in ammonium (<i<p</i< = 0.039). Based on these differences, a reliable and convenient assay for differentiating the two species was designed. This assay will be useful for identifying specific etiologic agents of aflatoxin contamination episodes in West Africa and other regions where the two species are sympatric, especially when phylogenetic analyses based on multiple gene segments are not practical. aflatoxins Aspergillus <i<Aspergillus aflatoxiformans</i< <i<Aspergillus minisclerotigenes</i< Medicine R Hillary L. Mehl verfasserin aut Marc J. Orbach verfasserin aut Kenneth A. Callicott verfasserin aut Peter J. Cotty verfasserin aut In Toxins MDPI AG, 2010 12(2020), 10, p 656 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:12 year:2020 number:10, p 656 https://doi.org/10.3390/toxins12100656 kostenfrei https://doaj.org/article/45f628ff4c17463eb47be85d11325442 kostenfrei https://www.mdpi.com/2072-6651/12/10/656 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 10, p 656 |
allfields_unstemmed |
10.3390/toxins12100656 doi (DE-627)DOAJ084652101 (DE-599)DOAJ45f628ff4c17463eb47be85d11325442 DE-627 ger DE-627 rakwb eng Pummi Singh verfasserin aut Phenotypic Differentiation of Two Morphologically Similar Aflatoxin-Producing Fungi from West Africa 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aflatoxins (AF) are hepatocarcinogenic metabolites produced by several <i<Aspergillus</i< species. Crop infection by these species results in aflatoxin contamination of cereals, nuts, and spices. Etiology of aflatoxin contamination is complicated by mixed infections of multiple species with similar morphology and aflatoxin profiles. The current study investigates variation in aflatoxin production between two morphologically similar species that co-exist in West Africa, <i<A. aflatoxiformans</i< and <i<A. minisclerotigenes</i<. Consistent distinctions in aflatoxin production during liquid fermentation were discovered between these species. The two species produced similar concentrations of AFB<sub<1</sub< in defined media with either urea or ammonium as the sole nitrogen source. However, production of both AFB<sub<1</sub< and AFG<sub<1</sub< were inhibited (<i<p</i< < 0.001) for <i<A. aflatoxiformans</i< in a yeast extract medium with sucrose. Although production of AFG<sub<1</sub< by both species was similar in urea, <i<A. minisclerotigenes</i< produced greater concentrations of AFG<sub<1</sub< in ammonium (<i<p</i< = 0.039). Based on these differences, a reliable and convenient assay for differentiating the two species was designed. This assay will be useful for identifying specific etiologic agents of aflatoxin contamination episodes in West Africa and other regions where the two species are sympatric, especially when phylogenetic analyses based on multiple gene segments are not practical. aflatoxins Aspergillus <i<Aspergillus aflatoxiformans</i< <i<Aspergillus minisclerotigenes</i< Medicine R Hillary L. Mehl verfasserin aut Marc J. Orbach verfasserin aut Kenneth A. Callicott verfasserin aut Peter J. Cotty verfasserin aut In Toxins MDPI AG, 2010 12(2020), 10, p 656 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:12 year:2020 number:10, p 656 https://doi.org/10.3390/toxins12100656 kostenfrei https://doaj.org/article/45f628ff4c17463eb47be85d11325442 kostenfrei https://www.mdpi.com/2072-6651/12/10/656 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 10, p 656 |
allfieldsGer |
10.3390/toxins12100656 doi (DE-627)DOAJ084652101 (DE-599)DOAJ45f628ff4c17463eb47be85d11325442 DE-627 ger DE-627 rakwb eng Pummi Singh verfasserin aut Phenotypic Differentiation of Two Morphologically Similar Aflatoxin-Producing Fungi from West Africa 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aflatoxins (AF) are hepatocarcinogenic metabolites produced by several <i<Aspergillus</i< species. Crop infection by these species results in aflatoxin contamination of cereals, nuts, and spices. Etiology of aflatoxin contamination is complicated by mixed infections of multiple species with similar morphology and aflatoxin profiles. The current study investigates variation in aflatoxin production between two morphologically similar species that co-exist in West Africa, <i<A. aflatoxiformans</i< and <i<A. minisclerotigenes</i<. Consistent distinctions in aflatoxin production during liquid fermentation were discovered between these species. The two species produced similar concentrations of AFB<sub<1</sub< in defined media with either urea or ammonium as the sole nitrogen source. However, production of both AFB<sub<1</sub< and AFG<sub<1</sub< were inhibited (<i<p</i< < 0.001) for <i<A. aflatoxiformans</i< in a yeast extract medium with sucrose. Although production of AFG<sub<1</sub< by both species was similar in urea, <i<A. minisclerotigenes</i< produced greater concentrations of AFG<sub<1</sub< in ammonium (<i<p</i< = 0.039). Based on these differences, a reliable and convenient assay for differentiating the two species was designed. This assay will be useful for identifying specific etiologic agents of aflatoxin contamination episodes in West Africa and other regions where the two species are sympatric, especially when phylogenetic analyses based on multiple gene segments are not practical. aflatoxins Aspergillus <i<Aspergillus aflatoxiformans</i< <i<Aspergillus minisclerotigenes</i< Medicine R Hillary L. Mehl verfasserin aut Marc J. Orbach verfasserin aut Kenneth A. Callicott verfasserin aut Peter J. Cotty verfasserin aut In Toxins MDPI AG, 2010 12(2020), 10, p 656 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:12 year:2020 number:10, p 656 https://doi.org/10.3390/toxins12100656 kostenfrei https://doaj.org/article/45f628ff4c17463eb47be85d11325442 kostenfrei https://www.mdpi.com/2072-6651/12/10/656 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 10, p 656 |
allfieldsSound |
10.3390/toxins12100656 doi (DE-627)DOAJ084652101 (DE-599)DOAJ45f628ff4c17463eb47be85d11325442 DE-627 ger DE-627 rakwb eng Pummi Singh verfasserin aut Phenotypic Differentiation of Two Morphologically Similar Aflatoxin-Producing Fungi from West Africa 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aflatoxins (AF) are hepatocarcinogenic metabolites produced by several <i<Aspergillus</i< species. Crop infection by these species results in aflatoxin contamination of cereals, nuts, and spices. Etiology of aflatoxin contamination is complicated by mixed infections of multiple species with similar morphology and aflatoxin profiles. The current study investigates variation in aflatoxin production between two morphologically similar species that co-exist in West Africa, <i<A. aflatoxiformans</i< and <i<A. minisclerotigenes</i<. Consistent distinctions in aflatoxin production during liquid fermentation were discovered between these species. The two species produced similar concentrations of AFB<sub<1</sub< in defined media with either urea or ammonium as the sole nitrogen source. However, production of both AFB<sub<1</sub< and AFG<sub<1</sub< were inhibited (<i<p</i< < 0.001) for <i<A. aflatoxiformans</i< in a yeast extract medium with sucrose. Although production of AFG<sub<1</sub< by both species was similar in urea, <i<A. minisclerotigenes</i< produced greater concentrations of AFG<sub<1</sub< in ammonium (<i<p</i< = 0.039). Based on these differences, a reliable and convenient assay for differentiating the two species was designed. This assay will be useful for identifying specific etiologic agents of aflatoxin contamination episodes in West Africa and other regions where the two species are sympatric, especially when phylogenetic analyses based on multiple gene segments are not practical. aflatoxins Aspergillus <i<Aspergillus aflatoxiformans</i< <i<Aspergillus minisclerotigenes</i< Medicine R Hillary L. Mehl verfasserin aut Marc J. Orbach verfasserin aut Kenneth A. Callicott verfasserin aut Peter J. Cotty verfasserin aut In Toxins MDPI AG, 2010 12(2020), 10, p 656 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:12 year:2020 number:10, p 656 https://doi.org/10.3390/toxins12100656 kostenfrei https://doaj.org/article/45f628ff4c17463eb47be85d11325442 kostenfrei https://www.mdpi.com/2072-6651/12/10/656 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 10, p 656 |
language |
English |
source |
In Toxins 12(2020), 10, p 656 volume:12 year:2020 number:10, p 656 |
sourceStr |
In Toxins 12(2020), 10, p 656 volume:12 year:2020 number:10, p 656 |
format_phy_str_mv |
Article |
institution |
findex.gbv.de |
topic_facet |
aflatoxins Aspergillus <i<Aspergillus aflatoxiformans</i< <i<Aspergillus minisclerotigenes</i< Medicine R |
isfreeaccess_bool |
true |
container_title |
Toxins |
authorswithroles_txt_mv |
Pummi Singh @@aut@@ Hillary L. Mehl @@aut@@ Marc J. Orbach @@aut@@ Kenneth A. Callicott @@aut@@ Peter J. Cotty @@aut@@ |
publishDateDaySort_date |
2020-01-01T00:00:00Z |
hierarchy_top_id |
610604236 |
id |
DOAJ084652101 |
language_de |
englisch |
fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ084652101</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240412212149.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230311s2020 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3390/toxins12100656</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ084652101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ45f628ff4c17463eb47be85d11325442</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Pummi Singh</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Phenotypic Differentiation of Two Morphologically Similar Aflatoxin-Producing Fungi from West Africa</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2020</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Aflatoxins (AF) are hepatocarcinogenic metabolites produced by several <i<Aspergillus</i< species. Crop infection by these species results in aflatoxin contamination of cereals, nuts, and spices. Etiology of aflatoxin contamination is complicated by mixed infections of multiple species with similar morphology and aflatoxin profiles. The current study investigates variation in aflatoxin production between two morphologically similar species that co-exist in West Africa, <i<A. aflatoxiformans</i< and <i<A. minisclerotigenes</i<. Consistent distinctions in aflatoxin production during liquid fermentation were discovered between these species. The two species produced similar concentrations of AFB<sub<1</sub< in defined media with either urea or ammonium as the sole nitrogen source. However, production of both AFB<sub<1</sub< and AFG<sub<1</sub< were inhibited (<i<p</i< < 0.001) for <i<A. aflatoxiformans</i< in a yeast extract medium with sucrose. Although production of AFG<sub<1</sub< by both species was similar in urea, <i<A. minisclerotigenes</i< produced greater concentrations of AFG<sub<1</sub< in ammonium (<i<p</i< = 0.039). Based on these differences, a reliable and convenient assay for differentiating the two species was designed. This assay will be useful for identifying specific etiologic agents of aflatoxin contamination episodes in West Africa and other regions where the two species are sympatric, especially when phylogenetic analyses based on multiple gene segments are not practical.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">aflatoxins</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Aspergillus</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><i<Aspergillus aflatoxiformans</i<</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><i<Aspergillus minisclerotigenes</i<</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Medicine</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">R</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Hillary L. Mehl</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Marc J. Orbach</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Kenneth A. Callicott</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Peter J. Cotty</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Toxins</subfield><subfield code="d">MDPI AG, 2010</subfield><subfield code="g">12(2020), 10, p 656</subfield><subfield code="w">(DE-627)610604236</subfield><subfield code="w">(DE-600)2518395-3</subfield><subfield code="x">20726651</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:12</subfield><subfield code="g">year:2020</subfield><subfield code="g">number:10, p 656</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.3390/toxins12100656</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/45f628ff4c17463eb47be85d11325442</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.mdpi.com/2072-6651/12/10/656</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2072-6651</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_224</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">12</subfield><subfield code="j">2020</subfield><subfield code="e">10, p 656</subfield></datafield></record></collection>
|
author |
Pummi Singh |
spellingShingle |
Pummi Singh misc aflatoxins misc Aspergillus misc <i<Aspergillus aflatoxiformans</i< misc <i<Aspergillus minisclerotigenes</i< misc Medicine misc R Phenotypic Differentiation of Two Morphologically Similar Aflatoxin-Producing Fungi from West Africa |
authorStr |
Pummi Singh |
ppnlink_with_tag_str_mv |
@@773@@(DE-627)610604236 |
format |
electronic Article |
delete_txt_mv |
keep |
author_role |
aut aut aut aut aut |
collection |
DOAJ |
remote_str |
true |
illustrated |
Not Illustrated |
issn |
20726651 |
topic_title |
Phenotypic Differentiation of Two Morphologically Similar Aflatoxin-Producing Fungi from West Africa aflatoxins Aspergillus <i<Aspergillus aflatoxiformans</i< <i<Aspergillus minisclerotigenes</i< |
topic |
misc aflatoxins misc Aspergillus misc <i<Aspergillus aflatoxiformans</i< misc <i<Aspergillus minisclerotigenes</i< misc Medicine misc R |
topic_unstemmed |
misc aflatoxins misc Aspergillus misc <i<Aspergillus aflatoxiformans</i< misc <i<Aspergillus minisclerotigenes</i< misc Medicine misc R |
topic_browse |
misc aflatoxins misc Aspergillus misc <i<Aspergillus aflatoxiformans</i< misc <i<Aspergillus minisclerotigenes</i< misc Medicine misc R |
format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
format_main_str_mv |
Text Zeitschrift/Artikel |
carriertype_str_mv |
cr |
hierarchy_parent_title |
Toxins |
hierarchy_parent_id |
610604236 |
hierarchy_top_title |
Toxins |
isfreeaccess_txt |
true |
familylinks_str_mv |
(DE-627)610604236 (DE-600)2518395-3 |
title |
Phenotypic Differentiation of Two Morphologically Similar Aflatoxin-Producing Fungi from West Africa |
ctrlnum |
(DE-627)DOAJ084652101 (DE-599)DOAJ45f628ff4c17463eb47be85d11325442 |
title_full |
Phenotypic Differentiation of Two Morphologically Similar Aflatoxin-Producing Fungi from West Africa |
author_sort |
Pummi Singh |
journal |
Toxins |
journalStr |
Toxins |
lang_code |
eng |
isOA_bool |
true |
recordtype |
marc |
publishDateSort |
2020 |
contenttype_str_mv |
txt |
author_browse |
Pummi Singh Hillary L. Mehl Marc J. Orbach Kenneth A. Callicott Peter J. Cotty |
container_volume |
12 |
format_se |
Elektronische Aufsätze |
author-letter |
Pummi Singh |
doi_str_mv |
10.3390/toxins12100656 |
author2-role |
verfasserin |
title_sort |
phenotypic differentiation of two morphologically similar aflatoxin-producing fungi from west africa |
title_auth |
Phenotypic Differentiation of Two Morphologically Similar Aflatoxin-Producing Fungi from West Africa |
abstract |
Aflatoxins (AF) are hepatocarcinogenic metabolites produced by several <i<Aspergillus</i< species. Crop infection by these species results in aflatoxin contamination of cereals, nuts, and spices. Etiology of aflatoxin contamination is complicated by mixed infections of multiple species with similar morphology and aflatoxin profiles. The current study investigates variation in aflatoxin production between two morphologically similar species that co-exist in West Africa, <i<A. aflatoxiformans</i< and <i<A. minisclerotigenes</i<. Consistent distinctions in aflatoxin production during liquid fermentation were discovered between these species. The two species produced similar concentrations of AFB<sub<1</sub< in defined media with either urea or ammonium as the sole nitrogen source. However, production of both AFB<sub<1</sub< and AFG<sub<1</sub< were inhibited (<i<p</i< < 0.001) for <i<A. aflatoxiformans</i< in a yeast extract medium with sucrose. Although production of AFG<sub<1</sub< by both species was similar in urea, <i<A. minisclerotigenes</i< produced greater concentrations of AFG<sub<1</sub< in ammonium (<i<p</i< = 0.039). Based on these differences, a reliable and convenient assay for differentiating the two species was designed. This assay will be useful for identifying specific etiologic agents of aflatoxin contamination episodes in West Africa and other regions where the two species are sympatric, especially when phylogenetic analyses based on multiple gene segments are not practical. |
abstractGer |
Aflatoxins (AF) are hepatocarcinogenic metabolites produced by several <i<Aspergillus</i< species. Crop infection by these species results in aflatoxin contamination of cereals, nuts, and spices. Etiology of aflatoxin contamination is complicated by mixed infections of multiple species with similar morphology and aflatoxin profiles. The current study investigates variation in aflatoxin production between two morphologically similar species that co-exist in West Africa, <i<A. aflatoxiformans</i< and <i<A. minisclerotigenes</i<. Consistent distinctions in aflatoxin production during liquid fermentation were discovered between these species. The two species produced similar concentrations of AFB<sub<1</sub< in defined media with either urea or ammonium as the sole nitrogen source. However, production of both AFB<sub<1</sub< and AFG<sub<1</sub< were inhibited (<i<p</i< < 0.001) for <i<A. aflatoxiformans</i< in a yeast extract medium with sucrose. Although production of AFG<sub<1</sub< by both species was similar in urea, <i<A. minisclerotigenes</i< produced greater concentrations of AFG<sub<1</sub< in ammonium (<i<p</i< = 0.039). Based on these differences, a reliable and convenient assay for differentiating the two species was designed. This assay will be useful for identifying specific etiologic agents of aflatoxin contamination episodes in West Africa and other regions where the two species are sympatric, especially when phylogenetic analyses based on multiple gene segments are not practical. |
abstract_unstemmed |
Aflatoxins (AF) are hepatocarcinogenic metabolites produced by several <i<Aspergillus</i< species. Crop infection by these species results in aflatoxin contamination of cereals, nuts, and spices. Etiology of aflatoxin contamination is complicated by mixed infections of multiple species with similar morphology and aflatoxin profiles. The current study investigates variation in aflatoxin production between two morphologically similar species that co-exist in West Africa, <i<A. aflatoxiformans</i< and <i<A. minisclerotigenes</i<. Consistent distinctions in aflatoxin production during liquid fermentation were discovered between these species. The two species produced similar concentrations of AFB<sub<1</sub< in defined media with either urea or ammonium as the sole nitrogen source. However, production of both AFB<sub<1</sub< and AFG<sub<1</sub< were inhibited (<i<p</i< < 0.001) for <i<A. aflatoxiformans</i< in a yeast extract medium with sucrose. Although production of AFG<sub<1</sub< by both species was similar in urea, <i<A. minisclerotigenes</i< produced greater concentrations of AFG<sub<1</sub< in ammonium (<i<p</i< = 0.039). Based on these differences, a reliable and convenient assay for differentiating the two species was designed. This assay will be useful for identifying specific etiologic agents of aflatoxin contamination episodes in West Africa and other regions where the two species are sympatric, especially when phylogenetic analyses based on multiple gene segments are not practical. |
collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 |
container_issue |
10, p 656 |
title_short |
Phenotypic Differentiation of Two Morphologically Similar Aflatoxin-Producing Fungi from West Africa |
url |
https://doi.org/10.3390/toxins12100656 https://doaj.org/article/45f628ff4c17463eb47be85d11325442 https://www.mdpi.com/2072-6651/12/10/656 https://doaj.org/toc/2072-6651 |
remote_bool |
true |
author2 |
Hillary L. Mehl Marc J. Orbach Kenneth A. Callicott Peter J. Cotty |
author2Str |
Hillary L. Mehl Marc J. Orbach Kenneth A. Callicott Peter J. Cotty |
ppnlink |
610604236 |
mediatype_str_mv |
c |
isOA_txt |
true |
hochschulschrift_bool |
false |
doi_str |
10.3390/toxins12100656 |
up_date |
2024-07-04T00:00:14.158Z |
_version_ |
1803604421939036160 |
fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ084652101</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240412212149.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230311s2020 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3390/toxins12100656</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ084652101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ45f628ff4c17463eb47be85d11325442</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Pummi Singh</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Phenotypic Differentiation of Two Morphologically Similar Aflatoxin-Producing Fungi from West Africa</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2020</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Aflatoxins (AF) are hepatocarcinogenic metabolites produced by several <i<Aspergillus</i< species. Crop infection by these species results in aflatoxin contamination of cereals, nuts, and spices. Etiology of aflatoxin contamination is complicated by mixed infections of multiple species with similar morphology and aflatoxin profiles. The current study investigates variation in aflatoxin production between two morphologically similar species that co-exist in West Africa, <i<A. aflatoxiformans</i< and <i<A. minisclerotigenes</i<. Consistent distinctions in aflatoxin production during liquid fermentation were discovered between these species. The two species produced similar concentrations of AFB<sub<1</sub< in defined media with either urea or ammonium as the sole nitrogen source. However, production of both AFB<sub<1</sub< and AFG<sub<1</sub< were inhibited (<i<p</i< < 0.001) for <i<A. aflatoxiformans</i< in a yeast extract medium with sucrose. Although production of AFG<sub<1</sub< by both species was similar in urea, <i<A. minisclerotigenes</i< produced greater concentrations of AFG<sub<1</sub< in ammonium (<i<p</i< = 0.039). Based on these differences, a reliable and convenient assay for differentiating the two species was designed. This assay will be useful for identifying specific etiologic agents of aflatoxin contamination episodes in West Africa and other regions where the two species are sympatric, especially when phylogenetic analyses based on multiple gene segments are not practical.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">aflatoxins</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Aspergillus</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><i<Aspergillus aflatoxiformans</i<</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><i<Aspergillus minisclerotigenes</i<</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Medicine</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">R</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Hillary L. Mehl</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Marc J. Orbach</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Kenneth A. Callicott</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Peter J. Cotty</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Toxins</subfield><subfield code="d">MDPI AG, 2010</subfield><subfield code="g">12(2020), 10, p 656</subfield><subfield code="w">(DE-627)610604236</subfield><subfield code="w">(DE-600)2518395-3</subfield><subfield code="x">20726651</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:12</subfield><subfield code="g">year:2020</subfield><subfield code="g">number:10, p 656</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.3390/toxins12100656</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/45f628ff4c17463eb47be85d11325442</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.mdpi.com/2072-6651/12/10/656</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2072-6651</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_224</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">12</subfield><subfield code="j">2020</subfield><subfield code="e">10, p 656</subfield></datafield></record></collection>
|
score |
7.399932 |