Bio-Guided Isolation of Antimalarial Metabolites from the Coculture of Two Red Sea Sponge-Derived <i<Actinokineospora</i< and <i<Rhodococcus</i< spp.

Coculture is a productive technique to trigger microbes’ biosynthetic capacity by mimicking the natural habitats’ features principally by competition for food and space and interspecies cross-talks. Mixed cultivation of two Red Sea-derived actinobacteria, <i<Actinokineospora spheciospongiae<...
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Autor*in:	Hani A. Alhadrami [verfasserIn] Bathini Thissera [verfasserIn] Marwa H. A. Hassan [verfasserIn] Fathy A. Behery [verfasserIn] Che Julius Ngwa [verfasserIn] Hossam M. Hassan [verfasserIn] Gabriele Pradel [verfasserIn] Usama Ramadan Abdelmohsen [verfasserIn] Mostafa E. Rateb [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	co-culture metabolomics antimalarial docking

Übergeordnetes Werk:	In: Marine Drugs - MDPI AG, 2005, 19(2021), 2, p 109
Übergeordnetes Werk:	volume:19 ; year:2021 ; number:2, p 109

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/md19020109

Katalog-ID:	DOAJ084708050

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allfields	10.3390/md19020109 doi (DE-627)DOAJ084708050 (DE-599)DOAJ0e8b5da6abe8431c81f75538f1540026 DE-627 ger DE-627 rakwb eng QH301-705.5 Hani A. Alhadrami verfasserin aut Bio-Guided Isolation of Antimalarial Metabolites from the Coculture of Two Red Sea Sponge-Derived <i<Actinokineospora</i< and <i<Rhodococcus</i< spp. 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Coculture is a productive technique to trigger microbes’ biosynthetic capacity by mimicking the natural habitats’ features principally by competition for food and space and interspecies cross-talks. Mixed cultivation of two Red Sea-derived actinobacteria, <i<Actinokineospora spheciospongiae</i< strain EG49 and <i<Rhodococcus</i< sp. UR59, resulted in the induction of several non-traced metabolites in their axenic cultures, which were detected using LC–HRMS metabolomics analysis. Antimalarial guided isolation of the cocultured fermentation led to the isolation of the angucyclines actinosporins E (<b<1</b<), H (<b<2</b<), G (<b<3</b<), tetragulol (<b<5</b<) and the anthraquinone capillasterquinone B (<b<6</b<), which were not reported under axenic conditions. Interestingly, actinosporins were previously induced when the axenic culture of the <i<Actinokineospora spheciospongiae</i< strain EG49 was treated with signalling molecule <i<N</i<-acetyl-<span style="font-variant: small-caps;"<d</span<-glucosamine (GluNAc); this finding confirmed the effectiveness of coculture in the discovery of microbial metabolites yet to be discovered in the axenic fermentation with the potential that could be comparable to adding chemical signalling molecules in the fermentation flask. The isolated angucycline and anthraquinone compounds exhibited in vitro antimalarial activity and good biding affinity against lysyl-tRNA synthetase (PfKRS1), highlighting their potential developability as new antimalarial structural motif. <i<Actinokineospora</i< <i<Rhodococcus</i< co-culture metabolomics antimalarial docking Biology (General) Bathini Thissera verfasserin aut Marwa H. A. Hassan verfasserin aut Fathy A. Behery verfasserin aut Che Julius Ngwa verfasserin aut Hossam M. Hassan verfasserin aut Gabriele Pradel verfasserin aut Usama Ramadan Abdelmohsen verfasserin aut Mostafa E. Rateb verfasserin aut In Marine Drugs MDPI AG, 2005 19(2021), 2, p 109 (DE-627)477992420 (DE-600)2175190-0 16603397 nnns volume:19 year:2021 number:2, p 109 https://doi.org/10.3390/md19020109 kostenfrei https://doaj.org/article/0e8b5da6abe8431c81f75538f1540026 kostenfrei https://www.mdpi.com/1660-3397/19/2/109 kostenfrei https://doaj.org/toc/1660-3397 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2021 2, p 109
spelling	10.3390/md19020109 doi (DE-627)DOAJ084708050 (DE-599)DOAJ0e8b5da6abe8431c81f75538f1540026 DE-627 ger DE-627 rakwb eng QH301-705.5 Hani A. Alhadrami verfasserin aut Bio-Guided Isolation of Antimalarial Metabolites from the Coculture of Two Red Sea Sponge-Derived <i<Actinokineospora</i< and <i<Rhodococcus</i< spp. 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Coculture is a productive technique to trigger microbes’ biosynthetic capacity by mimicking the natural habitats’ features principally by competition for food and space and interspecies cross-talks. Mixed cultivation of two Red Sea-derived actinobacteria, <i<Actinokineospora spheciospongiae</i< strain EG49 and <i<Rhodococcus</i< sp. UR59, resulted in the induction of several non-traced metabolites in their axenic cultures, which were detected using LC–HRMS metabolomics analysis. Antimalarial guided isolation of the cocultured fermentation led to the isolation of the angucyclines actinosporins E (<b<1</b<), H (<b<2</b<), G (<b<3</b<), tetragulol (<b<5</b<) and the anthraquinone capillasterquinone B (<b<6</b<), which were not reported under axenic conditions. Interestingly, actinosporins were previously induced when the axenic culture of the <i<Actinokineospora spheciospongiae</i< strain EG49 was treated with signalling molecule <i<N</i<-acetyl-<span style="font-variant: small-caps;"<d</span<-glucosamine (GluNAc); this finding confirmed the effectiveness of coculture in the discovery of microbial metabolites yet to be discovered in the axenic fermentation with the potential that could be comparable to adding chemical signalling molecules in the fermentation flask. The isolated angucycline and anthraquinone compounds exhibited in vitro antimalarial activity and good biding affinity against lysyl-tRNA synthetase (PfKRS1), highlighting their potential developability as new antimalarial structural motif. <i<Actinokineospora</i< <i<Rhodococcus</i< co-culture metabolomics antimalarial docking Biology (General) Bathini Thissera verfasserin aut Marwa H. A. Hassan verfasserin aut Fathy A. Behery verfasserin aut Che Julius Ngwa verfasserin aut Hossam M. Hassan verfasserin aut Gabriele Pradel verfasserin aut Usama Ramadan Abdelmohsen verfasserin aut Mostafa E. Rateb verfasserin aut In Marine Drugs MDPI AG, 2005 19(2021), 2, p 109 (DE-627)477992420 (DE-600)2175190-0 16603397 nnns volume:19 year:2021 number:2, p 109 https://doi.org/10.3390/md19020109 kostenfrei https://doaj.org/article/0e8b5da6abe8431c81f75538f1540026 kostenfrei https://www.mdpi.com/1660-3397/19/2/109 kostenfrei https://doaj.org/toc/1660-3397 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2021 2, p 109
allfields_unstemmed	10.3390/md19020109 doi (DE-627)DOAJ084708050 (DE-599)DOAJ0e8b5da6abe8431c81f75538f1540026 DE-627 ger DE-627 rakwb eng QH301-705.5 Hani A. Alhadrami verfasserin aut Bio-Guided Isolation of Antimalarial Metabolites from the Coculture of Two Red Sea Sponge-Derived <i<Actinokineospora</i< and <i<Rhodococcus</i< spp. 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Coculture is a productive technique to trigger microbes’ biosynthetic capacity by mimicking the natural habitats’ features principally by competition for food and space and interspecies cross-talks. Mixed cultivation of two Red Sea-derived actinobacteria, <i<Actinokineospora spheciospongiae</i< strain EG49 and <i<Rhodococcus</i< sp. UR59, resulted in the induction of several non-traced metabolites in their axenic cultures, which were detected using LC–HRMS metabolomics analysis. Antimalarial guided isolation of the cocultured fermentation led to the isolation of the angucyclines actinosporins E (<b<1</b<), H (<b<2</b<), G (<b<3</b<), tetragulol (<b<5</b<) and the anthraquinone capillasterquinone B (<b<6</b<), which were not reported under axenic conditions. Interestingly, actinosporins were previously induced when the axenic culture of the <i<Actinokineospora spheciospongiae</i< strain EG49 was treated with signalling molecule <i<N</i<-acetyl-<span style="font-variant: small-caps;"<d</span<-glucosamine (GluNAc); this finding confirmed the effectiveness of coculture in the discovery of microbial metabolites yet to be discovered in the axenic fermentation with the potential that could be comparable to adding chemical signalling molecules in the fermentation flask. The isolated angucycline and anthraquinone compounds exhibited in vitro antimalarial activity and good biding affinity against lysyl-tRNA synthetase (PfKRS1), highlighting their potential developability as new antimalarial structural motif. <i<Actinokineospora</i< <i<Rhodococcus</i< co-culture metabolomics antimalarial docking Biology (General) Bathini Thissera verfasserin aut Marwa H. A. Hassan verfasserin aut Fathy A. Behery verfasserin aut Che Julius Ngwa verfasserin aut Hossam M. Hassan verfasserin aut Gabriele Pradel verfasserin aut Usama Ramadan Abdelmohsen verfasserin aut Mostafa E. Rateb verfasserin aut In Marine Drugs MDPI AG, 2005 19(2021), 2, p 109 (DE-627)477992420 (DE-600)2175190-0 16603397 nnns volume:19 year:2021 number:2, p 109 https://doi.org/10.3390/md19020109 kostenfrei https://doaj.org/article/0e8b5da6abe8431c81f75538f1540026 kostenfrei https://www.mdpi.com/1660-3397/19/2/109 kostenfrei https://doaj.org/toc/1660-3397 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2021 2, p 109
allfieldsGer	10.3390/md19020109 doi (DE-627)DOAJ084708050 (DE-599)DOAJ0e8b5da6abe8431c81f75538f1540026 DE-627 ger DE-627 rakwb eng QH301-705.5 Hani A. Alhadrami verfasserin aut Bio-Guided Isolation of Antimalarial Metabolites from the Coculture of Two Red Sea Sponge-Derived <i<Actinokineospora</i< and <i<Rhodococcus</i< spp. 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Coculture is a productive technique to trigger microbes’ biosynthetic capacity by mimicking the natural habitats’ features principally by competition for food and space and interspecies cross-talks. Mixed cultivation of two Red Sea-derived actinobacteria, <i<Actinokineospora spheciospongiae</i< strain EG49 and <i<Rhodococcus</i< sp. UR59, resulted in the induction of several non-traced metabolites in their axenic cultures, which were detected using LC–HRMS metabolomics analysis. Antimalarial guided isolation of the cocultured fermentation led to the isolation of the angucyclines actinosporins E (<b<1</b<), H (<b<2</b<), G (<b<3</b<), tetragulol (<b<5</b<) and the anthraquinone capillasterquinone B (<b<6</b<), which were not reported under axenic conditions. Interestingly, actinosporins were previously induced when the axenic culture of the <i<Actinokineospora spheciospongiae</i< strain EG49 was treated with signalling molecule <i<N</i<-acetyl-<span style="font-variant: small-caps;"<d</span<-glucosamine (GluNAc); this finding confirmed the effectiveness of coculture in the discovery of microbial metabolites yet to be discovered in the axenic fermentation with the potential that could be comparable to adding chemical signalling molecules in the fermentation flask. The isolated angucycline and anthraquinone compounds exhibited in vitro antimalarial activity and good biding affinity against lysyl-tRNA synthetase (PfKRS1), highlighting their potential developability as new antimalarial structural motif. <i<Actinokineospora</i< <i<Rhodococcus</i< co-culture metabolomics antimalarial docking Biology (General) Bathini Thissera verfasserin aut Marwa H. A. Hassan verfasserin aut Fathy A. Behery verfasserin aut Che Julius Ngwa verfasserin aut Hossam M. Hassan verfasserin aut Gabriele Pradel verfasserin aut Usama Ramadan Abdelmohsen verfasserin aut Mostafa E. Rateb verfasserin aut In Marine Drugs MDPI AG, 2005 19(2021), 2, p 109 (DE-627)477992420 (DE-600)2175190-0 16603397 nnns volume:19 year:2021 number:2, p 109 https://doi.org/10.3390/md19020109 kostenfrei https://doaj.org/article/0e8b5da6abe8431c81f75538f1540026 kostenfrei https://www.mdpi.com/1660-3397/19/2/109 kostenfrei https://doaj.org/toc/1660-3397 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2021 2, p 109
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language	English
source	In Marine Drugs 19(2021), 2, p 109 volume:19 year:2021 number:2, p 109
sourceStr	In Marine Drugs 19(2021), 2, p 109 volume:19 year:2021 number:2, p 109
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<Actinokineospora</i< <i<Rhodococcus</i< co-culture metabolomics antimalarial docking Biology (General)
isfreeaccess_bool	true
container_title	Marine Drugs
authorswithroles_txt_mv	Hani A. Alhadrami @@aut@@ Bathini Thissera @@aut@@ Marwa H. A. Hassan @@aut@@ Fathy A. Behery @@aut@@ Che Julius Ngwa @@aut@@ Hossam M. Hassan @@aut@@ Gabriele Pradel @@aut@@ Usama Ramadan Abdelmohsen @@aut@@ Mostafa E. Rateb @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	477992420
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callnumber-first	Q - Science
author	Hani A. Alhadrami
spellingShingle	Hani A. Alhadrami misc QH301-705.5 misc <i<Actinokineospora</i< misc <i<Rhodococcus</i< misc co-culture misc metabolomics misc antimalarial misc docking misc Biology (General) Bio-Guided Isolation of Antimalarial Metabolites from the Coculture of Two Red Sea Sponge-Derived <i<Actinokineospora</i< and <i<Rhodococcus</i< spp.
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topic_title	QH301-705.5 Bio-Guided Isolation of Antimalarial Metabolites from the Coculture of Two Red Sea Sponge-Derived <i<Actinokineospora</i< and <i<Rhodococcus</i< spp <i<Actinokineospora</i< <i<Rhodococcus</i< co-culture metabolomics antimalarial docking
topic	misc QH301-705.5 misc <i<Actinokineospora</i< misc <i<Rhodococcus</i< misc co-culture misc metabolomics misc antimalarial misc docking misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc <i<Actinokineospora</i< misc <i<Rhodococcus</i< misc co-culture misc metabolomics misc antimalarial misc docking misc Biology (General)
topic_browse	misc QH301-705.5 misc <i<Actinokineospora</i< misc <i<Rhodococcus</i< misc co-culture misc metabolomics misc antimalarial misc docking misc Biology (General)
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title	Bio-Guided Isolation of Antimalarial Metabolites from the Coculture of Two Red Sea Sponge-Derived <i<Actinokineospora</i< and <i<Rhodococcus</i< spp.
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title_full	Bio-Guided Isolation of Antimalarial Metabolites from the Coculture of Two Red Sea Sponge-Derived <i<Actinokineospora</i< and <i<Rhodococcus</i< spp
author_sort	Hani A. Alhadrami
journal	Marine Drugs
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author_browse	Hani A. Alhadrami Bathini Thissera Marwa H. A. Hassan Fathy A. Behery Che Julius Ngwa Hossam M. Hassan Gabriele Pradel Usama Ramadan Abdelmohsen Mostafa E. Rateb
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author-letter	Hani A. Alhadrami
doi_str_mv	10.3390/md19020109
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title_sort	bio-guided isolation of antimalarial metabolites from the coculture of two red sea sponge-derived <i<actinokineospora</i< and <i<rhodococcus</i< spp
callnumber	QH301-705.5
title_auth	Bio-Guided Isolation of Antimalarial Metabolites from the Coculture of Two Red Sea Sponge-Derived <i<Actinokineospora</i< and <i<Rhodococcus</i< spp.
abstract	Coculture is a productive technique to trigger microbes’ biosynthetic capacity by mimicking the natural habitats’ features principally by competition for food and space and interspecies cross-talks. Mixed cultivation of two Red Sea-derived actinobacteria, <i<Actinokineospora spheciospongiae</i< strain EG49 and <i<Rhodococcus</i< sp. UR59, resulted in the induction of several non-traced metabolites in their axenic cultures, which were detected using LC–HRMS metabolomics analysis. Antimalarial guided isolation of the cocultured fermentation led to the isolation of the angucyclines actinosporins E (<b<1</b<), H (<b<2</b<), G (<b<3</b<), tetragulol (<b<5</b<) and the anthraquinone capillasterquinone B (<b<6</b<), which were not reported under axenic conditions. Interestingly, actinosporins were previously induced when the axenic culture of the <i<Actinokineospora spheciospongiae</i< strain EG49 was treated with signalling molecule <i<N</i<-acetyl-<span style="font-variant: small-caps;"<d</span<-glucosamine (GluNAc); this finding confirmed the effectiveness of coculture in the discovery of microbial metabolites yet to be discovered in the axenic fermentation with the potential that could be comparable to adding chemical signalling molecules in the fermentation flask. The isolated angucycline and anthraquinone compounds exhibited in vitro antimalarial activity and good biding affinity against lysyl-tRNA synthetase (PfKRS1), highlighting their potential developability as new antimalarial structural motif.
abstractGer	Coculture is a productive technique to trigger microbes’ biosynthetic capacity by mimicking the natural habitats’ features principally by competition for food and space and interspecies cross-talks. Mixed cultivation of two Red Sea-derived actinobacteria, <i<Actinokineospora spheciospongiae</i< strain EG49 and <i<Rhodococcus</i< sp. UR59, resulted in the induction of several non-traced metabolites in their axenic cultures, which were detected using LC–HRMS metabolomics analysis. Antimalarial guided isolation of the cocultured fermentation led to the isolation of the angucyclines actinosporins E (<b<1</b<), H (<b<2</b<), G (<b<3</b<), tetragulol (<b<5</b<) and the anthraquinone capillasterquinone B (<b<6</b<), which were not reported under axenic conditions. Interestingly, actinosporins were previously induced when the axenic culture of the <i<Actinokineospora spheciospongiae</i< strain EG49 was treated with signalling molecule <i<N</i<-acetyl-<span style="font-variant: small-caps;"<d</span<-glucosamine (GluNAc); this finding confirmed the effectiveness of coculture in the discovery of microbial metabolites yet to be discovered in the axenic fermentation with the potential that could be comparable to adding chemical signalling molecules in the fermentation flask. The isolated angucycline and anthraquinone compounds exhibited in vitro antimalarial activity and good biding affinity against lysyl-tRNA synthetase (PfKRS1), highlighting their potential developability as new antimalarial structural motif.
abstract_unstemmed	Coculture is a productive technique to trigger microbes’ biosynthetic capacity by mimicking the natural habitats’ features principally by competition for food and space and interspecies cross-talks. Mixed cultivation of two Red Sea-derived actinobacteria, <i<Actinokineospora spheciospongiae</i< strain EG49 and <i<Rhodococcus</i< sp. UR59, resulted in the induction of several non-traced metabolites in their axenic cultures, which were detected using LC–HRMS metabolomics analysis. Antimalarial guided isolation of the cocultured fermentation led to the isolation of the angucyclines actinosporins E (<b<1</b<), H (<b<2</b<), G (<b<3</b<), tetragulol (<b<5</b<) and the anthraquinone capillasterquinone B (<b<6</b<), which were not reported under axenic conditions. Interestingly, actinosporins were previously induced when the axenic culture of the <i<Actinokineospora spheciospongiae</i< strain EG49 was treated with signalling molecule <i<N</i<-acetyl-<span style="font-variant: small-caps;"<d</span<-glucosamine (GluNAc); this finding confirmed the effectiveness of coculture in the discovery of microbial metabolites yet to be discovered in the axenic fermentation with the potential that could be comparable to adding chemical signalling molecules in the fermentation flask. The isolated angucycline and anthraquinone compounds exhibited in vitro antimalarial activity and good biding affinity against lysyl-tRNA synthetase (PfKRS1), highlighting their potential developability as new antimalarial structural motif.
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container_issue	2, p 109
title_short	Bio-Guided Isolation of Antimalarial Metabolites from the Coculture of Two Red Sea Sponge-Derived <i<Actinokineospora</i< and <i<Rhodococcus</i< spp.
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author2	Bathini Thissera Marwa H. A. Hassan Fathy A. Behery Che Julius Ngwa Hossam M. Hassan Gabriele Pradel Usama Ramadan Abdelmohsen Mostafa E. Rateb
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Bio-Guided Isolation of Antimalarial Metabolites from the Coculture of Two Red Sea Sponge-Derived <i<Actinokineospora</i< and <i<Rhodococcus</i< spp.

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