NEW MONOCLONAL ANTIBODY FOR TUMOR-ASSOCIATED PROTEIN PMEPA1 DETECTION

We obtained a new monoclonal antibody (mAb) against tumor-associated protein PMEPA1 (Prostate Transmembrane Protein, Androgen Induced 1) for which is shown differential expression in some tumors. To test the specificity of obtained mAb we have produced a full-length recombinant PMEPA1 in transfected...
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Autor*in:	V. V. Volkomorov [verfasserIn] E. S. Grigoryeva [verfasserIn] M. S. Karbyshev [verfasserIn] I. V. Mitrofanofa [verfasserIn] E. V. Kaigorodova [verfasserIn] N. V. Cherdyntseva [verfasserIn]

Format:	E-Artikel
Sprache:	Russisch

Erschienen:	2016

Schlagwörter:	pmepa1 моноклональные антитела экспрессия белковые маркеры

Übergeordnetes Werk:	In: Сибирский онкологический журнал - Russian Academy of Sciences, Tomsk National Research Medical Center, 2017, (2016), 3, Seite 72-77
Übergeordnetes Werk:	year:2016 ; number:3 ; pages:72-77

Links:	Link aufrufen Link aufrufen Journal toc Journal toc

Katalog-ID:	DOAJ084722258

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allfields	(DE-627)DOAJ084722258 (DE-599)DOAJ5b57de74302247079a973de473155ede DE-627 ger DE-627 rakwb rus RC254-282 V. V. Volkomorov verfasserin aut NEW MONOCLONAL ANTIBODY FOR TUMOR-ASSOCIATED PROTEIN PMEPA1 DETECTION 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We obtained a new monoclonal antibody (mAb) against tumor-associated protein PMEPA1 (Prostate Transmembrane Protein, Androgen Induced 1) for which is shown differential expression in some tumors. To test the specificity of obtained mAb we have produced a full-length recombinant PMEPA1 in transfected HEK293T cell line. After transfection of the plasmid vector pIRES-EGFP, containing the insertion coding PMEPA1 gene we evaluated PMEPA1 protein expression in HEK293T cells lines by immunocytochemical study and western-blot analysis. Ability of the new mAb binds PMEPA1 in clinical specimens is shown by immunohistochemistry on histological sections of prostate tissue used as positive control. New antibody may provide a basis for development of commercial antibody test for evaluation of protein expression in human biological samples. pmepa1 моноклональные антитела экспрессия белковые маркеры Neoplasms. Tumors. Oncology. Including cancer and carcinogens E. S. Grigoryeva verfasserin aut M. S. Karbyshev verfasserin aut I. V. Mitrofanofa verfasserin aut E. V. Kaigorodova verfasserin aut N. V. Cherdyntseva verfasserin aut In Сибирский онкологический журнал Russian Academy of Sciences, Tomsk National Research Medical Center, 2017 (2016), 3, Seite 72-77 (DE-627)731335074 (DE-600)2693095-X 23123168 nnns year:2016 number:3 pages:72-77 https://doaj.org/article/5b57de74302247079a973de473155ede kostenfrei https://www.siboncoj.ru/jour/article/view/55 kostenfrei https://doaj.org/toc/1814-4861 Journal toc kostenfrei https://doaj.org/toc/2312-3168 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2016 3 72-77
spelling	(DE-627)DOAJ084722258 (DE-599)DOAJ5b57de74302247079a973de473155ede DE-627 ger DE-627 rakwb rus RC254-282 V. V. Volkomorov verfasserin aut NEW MONOCLONAL ANTIBODY FOR TUMOR-ASSOCIATED PROTEIN PMEPA1 DETECTION 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We obtained a new monoclonal antibody (mAb) against tumor-associated protein PMEPA1 (Prostate Transmembrane Protein, Androgen Induced 1) for which is shown differential expression in some tumors. To test the specificity of obtained mAb we have produced a full-length recombinant PMEPA1 in transfected HEK293T cell line. After transfection of the plasmid vector pIRES-EGFP, containing the insertion coding PMEPA1 gene we evaluated PMEPA1 protein expression in HEK293T cells lines by immunocytochemical study and western-blot analysis. Ability of the new mAb binds PMEPA1 in clinical specimens is shown by immunohistochemistry on histological sections of prostate tissue used as positive control. New antibody may provide a basis for development of commercial antibody test for evaluation of protein expression in human biological samples. pmepa1 моноклональные антитела экспрессия белковые маркеры Neoplasms. Tumors. Oncology. Including cancer and carcinogens E. S. Grigoryeva verfasserin aut M. S. Karbyshev verfasserin aut I. V. Mitrofanofa verfasserin aut E. V. Kaigorodova verfasserin aut N. V. Cherdyntseva verfasserin aut In Сибирский онкологический журнал Russian Academy of Sciences, Tomsk National Research Medical Center, 2017 (2016), 3, Seite 72-77 (DE-627)731335074 (DE-600)2693095-X 23123168 nnns year:2016 number:3 pages:72-77 https://doaj.org/article/5b57de74302247079a973de473155ede kostenfrei https://www.siboncoj.ru/jour/article/view/55 kostenfrei https://doaj.org/toc/1814-4861 Journal toc kostenfrei https://doaj.org/toc/2312-3168 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2016 3 72-77
allfields_unstemmed	(DE-627)DOAJ084722258 (DE-599)DOAJ5b57de74302247079a973de473155ede DE-627 ger DE-627 rakwb rus RC254-282 V. V. Volkomorov verfasserin aut NEW MONOCLONAL ANTIBODY FOR TUMOR-ASSOCIATED PROTEIN PMEPA1 DETECTION 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We obtained a new monoclonal antibody (mAb) against tumor-associated protein PMEPA1 (Prostate Transmembrane Protein, Androgen Induced 1) for which is shown differential expression in some tumors. To test the specificity of obtained mAb we have produced a full-length recombinant PMEPA1 in transfected HEK293T cell line. After transfection of the plasmid vector pIRES-EGFP, containing the insertion coding PMEPA1 gene we evaluated PMEPA1 protein expression in HEK293T cells lines by immunocytochemical study and western-blot analysis. Ability of the new mAb binds PMEPA1 in clinical specimens is shown by immunohistochemistry on histological sections of prostate tissue used as positive control. New antibody may provide a basis for development of commercial antibody test for evaluation of protein expression in human biological samples. pmepa1 моноклональные антитела экспрессия белковые маркеры Neoplasms. Tumors. Oncology. Including cancer and carcinogens E. S. Grigoryeva verfasserin aut M. S. Karbyshev verfasserin aut I. V. Mitrofanofa verfasserin aut E. V. Kaigorodova verfasserin aut N. V. Cherdyntseva verfasserin aut In Сибирский онкологический журнал Russian Academy of Sciences, Tomsk National Research Medical Center, 2017 (2016), 3, Seite 72-77 (DE-627)731335074 (DE-600)2693095-X 23123168 nnns year:2016 number:3 pages:72-77 https://doaj.org/article/5b57de74302247079a973de473155ede kostenfrei https://www.siboncoj.ru/jour/article/view/55 kostenfrei https://doaj.org/toc/1814-4861 Journal toc kostenfrei https://doaj.org/toc/2312-3168 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2016 3 72-77
allfieldsGer	(DE-627)DOAJ084722258 (DE-599)DOAJ5b57de74302247079a973de473155ede DE-627 ger DE-627 rakwb rus RC254-282 V. V. Volkomorov verfasserin aut NEW MONOCLONAL ANTIBODY FOR TUMOR-ASSOCIATED PROTEIN PMEPA1 DETECTION 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We obtained a new monoclonal antibody (mAb) against tumor-associated protein PMEPA1 (Prostate Transmembrane Protein, Androgen Induced 1) for which is shown differential expression in some tumors. To test the specificity of obtained mAb we have produced a full-length recombinant PMEPA1 in transfected HEK293T cell line. After transfection of the plasmid vector pIRES-EGFP, containing the insertion coding PMEPA1 gene we evaluated PMEPA1 protein expression in HEK293T cells lines by immunocytochemical study and western-blot analysis. Ability of the new mAb binds PMEPA1 in clinical specimens is shown by immunohistochemistry on histological sections of prostate tissue used as positive control. New antibody may provide a basis for development of commercial antibody test for evaluation of protein expression in human biological samples. pmepa1 моноклональные антитела экспрессия белковые маркеры Neoplasms. Tumors. Oncology. Including cancer and carcinogens E. S. Grigoryeva verfasserin aut M. S. Karbyshev verfasserin aut I. V. Mitrofanofa verfasserin aut E. V. Kaigorodova verfasserin aut N. V. Cherdyntseva verfasserin aut In Сибирский онкологический журнал Russian Academy of Sciences, Tomsk National Research Medical Center, 2017 (2016), 3, Seite 72-77 (DE-627)731335074 (DE-600)2693095-X 23123168 nnns year:2016 number:3 pages:72-77 https://doaj.org/article/5b57de74302247079a973de473155ede kostenfrei https://www.siboncoj.ru/jour/article/view/55 kostenfrei https://doaj.org/toc/1814-4861 Journal toc kostenfrei https://doaj.org/toc/2312-3168 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2016 3 72-77
allfieldsSound	(DE-627)DOAJ084722258 (DE-599)DOAJ5b57de74302247079a973de473155ede DE-627 ger DE-627 rakwb rus RC254-282 V. V. Volkomorov verfasserin aut NEW MONOCLONAL ANTIBODY FOR TUMOR-ASSOCIATED PROTEIN PMEPA1 DETECTION 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We obtained a new monoclonal antibody (mAb) against tumor-associated protein PMEPA1 (Prostate Transmembrane Protein, Androgen Induced 1) for which is shown differential expression in some tumors. To test the specificity of obtained mAb we have produced a full-length recombinant PMEPA1 in transfected HEK293T cell line. After transfection of the plasmid vector pIRES-EGFP, containing the insertion coding PMEPA1 gene we evaluated PMEPA1 protein expression in HEK293T cells lines by immunocytochemical study and western-blot analysis. Ability of the new mAb binds PMEPA1 in clinical specimens is shown by immunohistochemistry on histological sections of prostate tissue used as positive control. New antibody may provide a basis for development of commercial antibody test for evaluation of protein expression in human biological samples. pmepa1 моноклональные антитела экспрессия белковые маркеры Neoplasms. Tumors. Oncology. Including cancer and carcinogens E. S. Grigoryeva verfasserin aut M. S. Karbyshev verfasserin aut I. V. Mitrofanofa verfasserin aut E. V. Kaigorodova verfasserin aut N. V. Cherdyntseva verfasserin aut In Сибирский онкологический журнал Russian Academy of Sciences, Tomsk National Research Medical Center, 2017 (2016), 3, Seite 72-77 (DE-627)731335074 (DE-600)2693095-X 23123168 nnns year:2016 number:3 pages:72-77 https://doaj.org/article/5b57de74302247079a973de473155ede kostenfrei https://www.siboncoj.ru/jour/article/view/55 kostenfrei https://doaj.org/toc/1814-4861 Journal toc kostenfrei https://doaj.org/toc/2312-3168 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2016 3 72-77
language	Russian
source	In Сибирский онкологический журнал (2016), 3, Seite 72-77 year:2016 number:3 pages:72-77
sourceStr	In Сибирский онкологический журнал (2016), 3, Seite 72-77 year:2016 number:3 pages:72-77
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	pmepa1 моноклональные антитела экспрессия белковые маркеры Neoplasms. Tumors. Oncology. Including cancer and carcinogens
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callnumber-first	R - Medicine
author	V. V. Volkomorov
spellingShingle	V. V. Volkomorov misc RC254-282 misc pmepa1 misc моноклональные антитела misc экспрессия misc белковые маркеры misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens NEW MONOCLONAL ANTIBODY FOR TUMOR-ASSOCIATED PROTEIN PMEPA1 DETECTION
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topic_title	RC254-282 NEW MONOCLONAL ANTIBODY FOR TUMOR-ASSOCIATED PROTEIN PMEPA1 DETECTION pmepa1 моноклональные антитела экспрессия белковые маркеры
topic	misc RC254-282 misc pmepa1 misc моноклональные антитела misc экспрессия misc белковые маркеры misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
topic_unstemmed	misc RC254-282 misc pmepa1 misc моноклональные антитела misc экспрессия misc белковые маркеры misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
topic_browse	misc RC254-282 misc pmepa1 misc моноклональные антитела misc экспрессия misc белковые маркеры misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
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title	NEW MONOCLONAL ANTIBODY FOR TUMOR-ASSOCIATED PROTEIN PMEPA1 DETECTION
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title_full	NEW MONOCLONAL ANTIBODY FOR TUMOR-ASSOCIATED PROTEIN PMEPA1 DETECTION
author_sort	V. V. Volkomorov
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author_browse	V. V. Volkomorov E. S. Grigoryeva M. S. Karbyshev I. V. Mitrofanofa E. V. Kaigorodova N. V. Cherdyntseva
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author-letter	V. V. Volkomorov
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title_sort	new monoclonal antibody for tumor-associated protein pmepa1 detection
callnumber	RC254-282
title_auth	NEW MONOCLONAL ANTIBODY FOR TUMOR-ASSOCIATED PROTEIN PMEPA1 DETECTION
abstract	We obtained a new monoclonal antibody (mAb) against tumor-associated protein PMEPA1 (Prostate Transmembrane Protein, Androgen Induced 1) for which is shown differential expression in some tumors. To test the specificity of obtained mAb we have produced a full-length recombinant PMEPA1 in transfected HEK293T cell line. After transfection of the plasmid vector pIRES-EGFP, containing the insertion coding PMEPA1 gene we evaluated PMEPA1 protein expression in HEK293T cells lines by immunocytochemical study and western-blot analysis. Ability of the new mAb binds PMEPA1 in clinical specimens is shown by immunohistochemistry on histological sections of prostate tissue used as positive control. New antibody may provide a basis for development of commercial antibody test for evaluation of protein expression in human biological samples.
abstractGer	We obtained a new monoclonal antibody (mAb) against tumor-associated protein PMEPA1 (Prostate Transmembrane Protein, Androgen Induced 1) for which is shown differential expression in some tumors. To test the specificity of obtained mAb we have produced a full-length recombinant PMEPA1 in transfected HEK293T cell line. After transfection of the plasmid vector pIRES-EGFP, containing the insertion coding PMEPA1 gene we evaluated PMEPA1 protein expression in HEK293T cells lines by immunocytochemical study and western-blot analysis. Ability of the new mAb binds PMEPA1 in clinical specimens is shown by immunohistochemistry on histological sections of prostate tissue used as positive control. New antibody may provide a basis for development of commercial antibody test for evaluation of protein expression in human biological samples.
abstract_unstemmed	We obtained a new monoclonal antibody (mAb) against tumor-associated protein PMEPA1 (Prostate Transmembrane Protein, Androgen Induced 1) for which is shown differential expression in some tumors. To test the specificity of obtained mAb we have produced a full-length recombinant PMEPA1 in transfected HEK293T cell line. After transfection of the plasmid vector pIRES-EGFP, containing the insertion coding PMEPA1 gene we evaluated PMEPA1 protein expression in HEK293T cells lines by immunocytochemical study and western-blot analysis. Ability of the new mAb binds PMEPA1 in clinical specimens is shown by immunohistochemistry on histological sections of prostate tissue used as positive control. New antibody may provide a basis for development of commercial antibody test for evaluation of protein expression in human biological samples.
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title_short	NEW MONOCLONAL ANTIBODY FOR TUMOR-ASSOCIATED PROTEIN PMEPA1 DETECTION
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