A <i<Bacillus thuringiensis</i< Chitin-Binding Protein is Involved in Insect Peritrophic Matrix Adhesion and Takes Part in the Infection Process

<i<Bacillus thuringiensis</i< (Bt) is used for insect pest control, and its larvicidal activity is primarily attributed to Cry toxins. Other factors participate in infection, and limited information is available regarding factors acting on the peritrophic matrix (PM). This study aimed to...
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Gespeichert in:

Autor*in:	Jiaxin Qin [verfasserIn] Zongxing Tong [verfasserIn] Yiling Zhan [verfasserIn] Christophe Buisson [verfasserIn] Fuping Song [verfasserIn] Kanglai He [verfasserIn] Christina Nielsen-LeRoux [verfasserIn] Shuyuan Guo [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	chitin-binding protein adhesion peritrophic matrix

Übergeordnetes Werk:	In: Toxins - MDPI AG, 2010, 12(2020), 4, p 252
Übergeordnetes Werk:	volume:12 ; year:2020 ; number:4, p 252

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/toxins12040252

Katalog-ID:	DOAJ08473535X

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allfields	10.3390/toxins12040252 doi (DE-627)DOAJ08473535X (DE-599)DOAJc94d13d02f934a299fb0b826deb5b24a DE-627 ger DE-627 rakwb eng Jiaxin Qin verfasserin aut A <i<Bacillus thuringiensis</i< Chitin-Binding Protein is Involved in Insect Peritrophic Matrix Adhesion and Takes Part in the Infection Process 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Bacillus thuringiensis</i< (Bt) is used for insect pest control, and its larvicidal activity is primarily attributed to Cry toxins. Other factors participate in infection, and limited information is available regarding factors acting on the peritrophic matrix (PM). This study aimed to investigate the role of a Bt chitin-binding protein (CBPA) that had been previously shown to be expressed at pH 9 <i<in vitro</i< and could therefore be expressed in the alkaline gut of lepidopteron larvae. A ∆cbpA mutant was generated that was 10-fold less virulent than wild-type Bt HD73 towards <i<Ostrinia furnacalis</i< neonate larvae, indicating its important role in infection. Purified recombinant <i<Escherichia coli</i< CBPA was shown to have a chitin affinity, thus indicating a possible interaction with the chitin-rich PM. A translational GFP–CBPA fusion elucidated the localization of CBPA on the bacterial surface, and the transcriptional activity of the promoter P<i<cbpA</i< was immediately induced and confirmed at pH 9. Next, in order to connect surface expression and possible <i<in vivo</i< gut activity<i<,</i< last instar <i<Galleria</i< <i<mellonella</i< (Gm) larvae (not susceptible to Bt HD-73) were used as a model to follow CBPA in gut expression, bacterial transit, and PM adhesion. CBPA-GFP was quickly expressed in the Gm gut lumen, and more Bt HD73 strain bacteria adhered to the PM than those of the ∆cbpA mutant strain. Therefore, CBPA may help to retain the bacteria, via the PM binding, close to the gut surface and thus takes part in the early steps of Bt gut interactions. <i<Bacillus thuringiensis</i< chitin-binding protein adhesion peritrophic matrix Medicine R Zongxing Tong verfasserin aut Yiling Zhan verfasserin aut Christophe Buisson verfasserin aut Fuping Song verfasserin aut Kanglai He verfasserin aut Christina Nielsen-LeRoux verfasserin aut Shuyuan Guo verfasserin aut In Toxins MDPI AG, 2010 12(2020), 4, p 252 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:12 year:2020 number:4, p 252 https://doi.org/10.3390/toxins12040252 kostenfrei https://doaj.org/article/c94d13d02f934a299fb0b826deb5b24a kostenfrei https://www.mdpi.com/2072-6651/12/4/252 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 4, p 252
spelling	10.3390/toxins12040252 doi (DE-627)DOAJ08473535X (DE-599)DOAJc94d13d02f934a299fb0b826deb5b24a DE-627 ger DE-627 rakwb eng Jiaxin Qin verfasserin aut A <i<Bacillus thuringiensis</i< Chitin-Binding Protein is Involved in Insect Peritrophic Matrix Adhesion and Takes Part in the Infection Process 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Bacillus thuringiensis</i< (Bt) is used for insect pest control, and its larvicidal activity is primarily attributed to Cry toxins. Other factors participate in infection, and limited information is available regarding factors acting on the peritrophic matrix (PM). This study aimed to investigate the role of a Bt chitin-binding protein (CBPA) that had been previously shown to be expressed at pH 9 <i<in vitro</i< and could therefore be expressed in the alkaline gut of lepidopteron larvae. A ∆cbpA mutant was generated that was 10-fold less virulent than wild-type Bt HD73 towards <i<Ostrinia furnacalis</i< neonate larvae, indicating its important role in infection. Purified recombinant <i<Escherichia coli</i< CBPA was shown to have a chitin affinity, thus indicating a possible interaction with the chitin-rich PM. A translational GFP–CBPA fusion elucidated the localization of CBPA on the bacterial surface, and the transcriptional activity of the promoter P<i<cbpA</i< was immediately induced and confirmed at pH 9. Next, in order to connect surface expression and possible <i<in vivo</i< gut activity<i<,</i< last instar <i<Galleria</i< <i<mellonella</i< (Gm) larvae (not susceptible to Bt HD-73) were used as a model to follow CBPA in gut expression, bacterial transit, and PM adhesion. CBPA-GFP was quickly expressed in the Gm gut lumen, and more Bt HD73 strain bacteria adhered to the PM than those of the ∆cbpA mutant strain. Therefore, CBPA may help to retain the bacteria, via the PM binding, close to the gut surface and thus takes part in the early steps of Bt gut interactions. <i<Bacillus thuringiensis</i< chitin-binding protein adhesion peritrophic matrix Medicine R Zongxing Tong verfasserin aut Yiling Zhan verfasserin aut Christophe Buisson verfasserin aut Fuping Song verfasserin aut Kanglai He verfasserin aut Christina Nielsen-LeRoux verfasserin aut Shuyuan Guo verfasserin aut In Toxins MDPI AG, 2010 12(2020), 4, p 252 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:12 year:2020 number:4, p 252 https://doi.org/10.3390/toxins12040252 kostenfrei https://doaj.org/article/c94d13d02f934a299fb0b826deb5b24a kostenfrei https://www.mdpi.com/2072-6651/12/4/252 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 4, p 252
allfields_unstemmed	10.3390/toxins12040252 doi (DE-627)DOAJ08473535X (DE-599)DOAJc94d13d02f934a299fb0b826deb5b24a DE-627 ger DE-627 rakwb eng Jiaxin Qin verfasserin aut A <i<Bacillus thuringiensis</i< Chitin-Binding Protein is Involved in Insect Peritrophic Matrix Adhesion and Takes Part in the Infection Process 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Bacillus thuringiensis</i< (Bt) is used for insect pest control, and its larvicidal activity is primarily attributed to Cry toxins. Other factors participate in infection, and limited information is available regarding factors acting on the peritrophic matrix (PM). This study aimed to investigate the role of a Bt chitin-binding protein (CBPA) that had been previously shown to be expressed at pH 9 <i<in vitro</i< and could therefore be expressed in the alkaline gut of lepidopteron larvae. A ∆cbpA mutant was generated that was 10-fold less virulent than wild-type Bt HD73 towards <i<Ostrinia furnacalis</i< neonate larvae, indicating its important role in infection. Purified recombinant <i<Escherichia coli</i< CBPA was shown to have a chitin affinity, thus indicating a possible interaction with the chitin-rich PM. A translational GFP–CBPA fusion elucidated the localization of CBPA on the bacterial surface, and the transcriptional activity of the promoter P<i<cbpA</i< was immediately induced and confirmed at pH 9. Next, in order to connect surface expression and possible <i<in vivo</i< gut activity<i<,</i< last instar <i<Galleria</i< <i<mellonella</i< (Gm) larvae (not susceptible to Bt HD-73) were used as a model to follow CBPA in gut expression, bacterial transit, and PM adhesion. CBPA-GFP was quickly expressed in the Gm gut lumen, and more Bt HD73 strain bacteria adhered to the PM than those of the ∆cbpA mutant strain. Therefore, CBPA may help to retain the bacteria, via the PM binding, close to the gut surface and thus takes part in the early steps of Bt gut interactions. <i<Bacillus thuringiensis</i< chitin-binding protein adhesion peritrophic matrix Medicine R Zongxing Tong verfasserin aut Yiling Zhan verfasserin aut Christophe Buisson verfasserin aut Fuping Song verfasserin aut Kanglai He verfasserin aut Christina Nielsen-LeRoux verfasserin aut Shuyuan Guo verfasserin aut In Toxins MDPI AG, 2010 12(2020), 4, p 252 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:12 year:2020 number:4, p 252 https://doi.org/10.3390/toxins12040252 kostenfrei https://doaj.org/article/c94d13d02f934a299fb0b826deb5b24a kostenfrei https://www.mdpi.com/2072-6651/12/4/252 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 4, p 252
allfieldsGer	10.3390/toxins12040252 doi (DE-627)DOAJ08473535X (DE-599)DOAJc94d13d02f934a299fb0b826deb5b24a DE-627 ger DE-627 rakwb eng Jiaxin Qin verfasserin aut A <i<Bacillus thuringiensis</i< Chitin-Binding Protein is Involved in Insect Peritrophic Matrix Adhesion and Takes Part in the Infection Process 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Bacillus thuringiensis</i< (Bt) is used for insect pest control, and its larvicidal activity is primarily attributed to Cry toxins. Other factors participate in infection, and limited information is available regarding factors acting on the peritrophic matrix (PM). This study aimed to investigate the role of a Bt chitin-binding protein (CBPA) that had been previously shown to be expressed at pH 9 <i<in vitro</i< and could therefore be expressed in the alkaline gut of lepidopteron larvae. A ∆cbpA mutant was generated that was 10-fold less virulent than wild-type Bt HD73 towards <i<Ostrinia furnacalis</i< neonate larvae, indicating its important role in infection. Purified recombinant <i<Escherichia coli</i< CBPA was shown to have a chitin affinity, thus indicating a possible interaction with the chitin-rich PM. A translational GFP–CBPA fusion elucidated the localization of CBPA on the bacterial surface, and the transcriptional activity of the promoter P<i<cbpA</i< was immediately induced and confirmed at pH 9. Next, in order to connect surface expression and possible <i<in vivo</i< gut activity<i<,</i< last instar <i<Galleria</i< <i<mellonella</i< (Gm) larvae (not susceptible to Bt HD-73) were used as a model to follow CBPA in gut expression, bacterial transit, and PM adhesion. CBPA-GFP was quickly expressed in the Gm gut lumen, and more Bt HD73 strain bacteria adhered to the PM than those of the ∆cbpA mutant strain. Therefore, CBPA may help to retain the bacteria, via the PM binding, close to the gut surface and thus takes part in the early steps of Bt gut interactions. <i<Bacillus thuringiensis</i< chitin-binding protein adhesion peritrophic matrix Medicine R Zongxing Tong verfasserin aut Yiling Zhan verfasserin aut Christophe Buisson verfasserin aut Fuping Song verfasserin aut Kanglai He verfasserin aut Christina Nielsen-LeRoux verfasserin aut Shuyuan Guo verfasserin aut In Toxins MDPI AG, 2010 12(2020), 4, p 252 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:12 year:2020 number:4, p 252 https://doi.org/10.3390/toxins12040252 kostenfrei https://doaj.org/article/c94d13d02f934a299fb0b826deb5b24a kostenfrei https://www.mdpi.com/2072-6651/12/4/252 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 4, p 252
allfieldsSound	10.3390/toxins12040252 doi (DE-627)DOAJ08473535X (DE-599)DOAJc94d13d02f934a299fb0b826deb5b24a DE-627 ger DE-627 rakwb eng Jiaxin Qin verfasserin aut A <i<Bacillus thuringiensis</i< Chitin-Binding Protein is Involved in Insect Peritrophic Matrix Adhesion and Takes Part in the Infection Process 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Bacillus thuringiensis</i< (Bt) is used for insect pest control, and its larvicidal activity is primarily attributed to Cry toxins. Other factors participate in infection, and limited information is available regarding factors acting on the peritrophic matrix (PM). This study aimed to investigate the role of a Bt chitin-binding protein (CBPA) that had been previously shown to be expressed at pH 9 <i<in vitro</i< and could therefore be expressed in the alkaline gut of lepidopteron larvae. A ∆cbpA mutant was generated that was 10-fold less virulent than wild-type Bt HD73 towards <i<Ostrinia furnacalis</i< neonate larvae, indicating its important role in infection. Purified recombinant <i<Escherichia coli</i< CBPA was shown to have a chitin affinity, thus indicating a possible interaction with the chitin-rich PM. A translational GFP–CBPA fusion elucidated the localization of CBPA on the bacterial surface, and the transcriptional activity of the promoter P<i<cbpA</i< was immediately induced and confirmed at pH 9. Next, in order to connect surface expression and possible <i<in vivo</i< gut activity<i<,</i< last instar <i<Galleria</i< <i<mellonella</i< (Gm) larvae (not susceptible to Bt HD-73) were used as a model to follow CBPA in gut expression, bacterial transit, and PM adhesion. CBPA-GFP was quickly expressed in the Gm gut lumen, and more Bt HD73 strain bacteria adhered to the PM than those of the ∆cbpA mutant strain. Therefore, CBPA may help to retain the bacteria, via the PM binding, close to the gut surface and thus takes part in the early steps of Bt gut interactions. <i<Bacillus thuringiensis</i< chitin-binding protein adhesion peritrophic matrix Medicine R Zongxing Tong verfasserin aut Yiling Zhan verfasserin aut Christophe Buisson verfasserin aut Fuping Song verfasserin aut Kanglai He verfasserin aut Christina Nielsen-LeRoux verfasserin aut Shuyuan Guo verfasserin aut In Toxins MDPI AG, 2010 12(2020), 4, p 252 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:12 year:2020 number:4, p 252 https://doi.org/10.3390/toxins12040252 kostenfrei https://doaj.org/article/c94d13d02f934a299fb0b826deb5b24a kostenfrei https://www.mdpi.com/2072-6651/12/4/252 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 4, p 252
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source	In Toxins 12(2020), 4, p 252 volume:12 year:2020 number:4, p 252
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topic_title	A <i<Bacillus thuringiensis</i< Chitin-Binding Protein is Involved in Insect Peritrophic Matrix Adhesion and Takes Part in the Infection Process <i<Bacillus thuringiensis</i< chitin-binding protein adhesion peritrophic matrix
topic	misc <i<Bacillus thuringiensis</i< misc chitin-binding protein misc adhesion misc peritrophic matrix misc Medicine misc R
topic_unstemmed	misc <i<Bacillus thuringiensis</i< misc chitin-binding protein misc adhesion misc peritrophic matrix misc Medicine misc R
topic_browse	misc <i<Bacillus thuringiensis</i< misc chitin-binding protein misc adhesion misc peritrophic matrix misc Medicine misc R
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title	A <i<Bacillus thuringiensis</i< Chitin-Binding Protein is Involved in Insect Peritrophic Matrix Adhesion and Takes Part in the Infection Process
ctrlnum	(DE-627)DOAJ08473535X (DE-599)DOAJc94d13d02f934a299fb0b826deb5b24a
title_full	A <i<Bacillus thuringiensis</i< Chitin-Binding Protein is Involved in Insect Peritrophic Matrix Adhesion and Takes Part in the Infection Process
author_sort	Jiaxin Qin
journal	Toxins
journalStr	Toxins
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author_browse	Jiaxin Qin Zongxing Tong Yiling Zhan Christophe Buisson Fuping Song Kanglai He Christina Nielsen-LeRoux Shuyuan Guo
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author-letter	Jiaxin Qin
doi_str_mv	10.3390/toxins12040252
author2-role	verfasserin
title_sort	<i<bacillus thuringiensis</i< chitin-binding protein is involved in insect peritrophic matrix adhesion and takes part in the infection process
title_auth	A <i<Bacillus thuringiensis</i< Chitin-Binding Protein is Involved in Insect Peritrophic Matrix Adhesion and Takes Part in the Infection Process
abstract	<i<Bacillus thuringiensis</i< (Bt) is used for insect pest control, and its larvicidal activity is primarily attributed to Cry toxins. Other factors participate in infection, and limited information is available regarding factors acting on the peritrophic matrix (PM). This study aimed to investigate the role of a Bt chitin-binding protein (CBPA) that had been previously shown to be expressed at pH 9 <i<in vitro</i< and could therefore be expressed in the alkaline gut of lepidopteron larvae. A ∆cbpA mutant was generated that was 10-fold less virulent than wild-type Bt HD73 towards <i<Ostrinia furnacalis</i< neonate larvae, indicating its important role in infection. Purified recombinant <i<Escherichia coli</i< CBPA was shown to have a chitin affinity, thus indicating a possible interaction with the chitin-rich PM. A translational GFP–CBPA fusion elucidated the localization of CBPA on the bacterial surface, and the transcriptional activity of the promoter P<i<cbpA</i< was immediately induced and confirmed at pH 9. Next, in order to connect surface expression and possible <i<in vivo</i< gut activity<i<,</i< last instar <i<Galleria</i< <i<mellonella</i< (Gm) larvae (not susceptible to Bt HD-73) were used as a model to follow CBPA in gut expression, bacterial transit, and PM adhesion. CBPA-GFP was quickly expressed in the Gm gut lumen, and more Bt HD73 strain bacteria adhered to the PM than those of the ∆cbpA mutant strain. Therefore, CBPA may help to retain the bacteria, via the PM binding, close to the gut surface and thus takes part in the early steps of Bt gut interactions.
abstractGer	<i<Bacillus thuringiensis</i< (Bt) is used for insect pest control, and its larvicidal activity is primarily attributed to Cry toxins. Other factors participate in infection, and limited information is available regarding factors acting on the peritrophic matrix (PM). This study aimed to investigate the role of a Bt chitin-binding protein (CBPA) that had been previously shown to be expressed at pH 9 <i<in vitro</i< and could therefore be expressed in the alkaline gut of lepidopteron larvae. A ∆cbpA mutant was generated that was 10-fold less virulent than wild-type Bt HD73 towards <i<Ostrinia furnacalis</i< neonate larvae, indicating its important role in infection. Purified recombinant <i<Escherichia coli</i< CBPA was shown to have a chitin affinity, thus indicating a possible interaction with the chitin-rich PM. A translational GFP–CBPA fusion elucidated the localization of CBPA on the bacterial surface, and the transcriptional activity of the promoter P<i<cbpA</i< was immediately induced and confirmed at pH 9. Next, in order to connect surface expression and possible <i<in vivo</i< gut activity<i<,</i< last instar <i<Galleria</i< <i<mellonella</i< (Gm) larvae (not susceptible to Bt HD-73) were used as a model to follow CBPA in gut expression, bacterial transit, and PM adhesion. CBPA-GFP was quickly expressed in the Gm gut lumen, and more Bt HD73 strain bacteria adhered to the PM than those of the ∆cbpA mutant strain. Therefore, CBPA may help to retain the bacteria, via the PM binding, close to the gut surface and thus takes part in the early steps of Bt gut interactions.
abstract_unstemmed	<i<Bacillus thuringiensis</i< (Bt) is used for insect pest control, and its larvicidal activity is primarily attributed to Cry toxins. Other factors participate in infection, and limited information is available regarding factors acting on the peritrophic matrix (PM). This study aimed to investigate the role of a Bt chitin-binding protein (CBPA) that had been previously shown to be expressed at pH 9 <i<in vitro</i< and could therefore be expressed in the alkaline gut of lepidopteron larvae. A ∆cbpA mutant was generated that was 10-fold less virulent than wild-type Bt HD73 towards <i<Ostrinia furnacalis</i< neonate larvae, indicating its important role in infection. Purified recombinant <i<Escherichia coli</i< CBPA was shown to have a chitin affinity, thus indicating a possible interaction with the chitin-rich PM. A translational GFP–CBPA fusion elucidated the localization of CBPA on the bacterial surface, and the transcriptional activity of the promoter P<i<cbpA</i< was immediately induced and confirmed at pH 9. Next, in order to connect surface expression and possible <i<in vivo</i< gut activity<i<,</i< last instar <i<Galleria</i< <i<mellonella</i< (Gm) larvae (not susceptible to Bt HD-73) were used as a model to follow CBPA in gut expression, bacterial transit, and PM adhesion. CBPA-GFP was quickly expressed in the Gm gut lumen, and more Bt HD73 strain bacteria adhered to the PM than those of the ∆cbpA mutant strain. Therefore, CBPA may help to retain the bacteria, via the PM binding, close to the gut surface and thus takes part in the early steps of Bt gut interactions.
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container_issue	4, p 252
title_short	A <i<Bacillus thuringiensis</i< Chitin-Binding Protein is Involved in Insect Peritrophic Matrix Adhesion and Takes Part in the Infection Process
url	https://doi.org/10.3390/toxins12040252 https://doaj.org/article/c94d13d02f934a299fb0b826deb5b24a https://www.mdpi.com/2072-6651/12/4/252 https://doaj.org/toc/2072-6651
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author2	Zongxing Tong Yiling Zhan Christophe Buisson Fuping Song Kanglai He Christina Nielsen-LeRoux Shuyuan Guo
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up_date	2024-07-04T00:19:08.494Z
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