HSP27 Attenuates cGAS-Mediated IFN-β Signaling through Ubiquitination of cGAS and Promotes PRV Infection

Pseudorabies (PR) is a domestic and wild animal infectious disease caused by the pseudorabies virus (PRV) and is one of the major infectious diseases that endanger the global swine industry. Studies have reported that PRV may achieve cross-species transmission from pigs to humans in recent years. Th...
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Autor*in:	Xiangrong Li [verfasserIn] Jingying Xie [verfasserIn] Dianyu Li [verfasserIn] Hongshan Li [verfasserIn] Yuhui Niu [verfasserIn] Bei Wu [verfasserIn] Yanmei Yang [verfasserIn] Zhenfang Yan [verfasserIn] Xiangbo Zhang [verfasserIn] Lei Chen [verfasserIn] Ruofei Feng [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	pseudorabies virus heat shock protein 27 cyclic GMP-AMP synthase cGAS-STING signaling pathway ubiquitination

Übergeordnetes Werk:	In: Viruses - MDPI AG, 2009, 14(2022), 9, p 1851
Übergeordnetes Werk:	volume:14 ; year:2022 ; number:9, p 1851

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/v14091851

Katalog-ID:	DOAJ084793309

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520			\|a Pseudorabies (PR) is a domestic and wild animal infectious disease caused by the pseudorabies virus (PRV) and is one of the major infectious diseases that endanger the global swine industry. Studies have reported that PRV may achieve cross-species transmission from pigs to humans in recent years. Therefore, in-depth exploration of the relationship between PRV and host proteins is of great significance for elucidating the pathogenic mechanism of PRV and anti-PRV infection. Here, we report that heat shock protein 27 (HSP27) ubiquitinates and degrades cyclic GMP-AMP synthase (cGAS) and attenuates cGAS-mediated antiviral responses, thereby promoting PRV infection. Overexpression of HSP27 promoted PRV proliferation in vitro, while knockdown of HSP27 inhibited PRV infection. Importantly, we found that HSP27 inhibited PRV infection or poly(dA:dT)-activated IFN-β expression. Further studies found that HSP27 may inhibit cGAS-STING-mediated IFN-β expression through targeting cGAS. In addition, we found that HSP27 can suppress the expression of endogenous cGAS in different cells at both gene transcription and protein expression levels, and that HSP27 interacts with and ubiquitinates cGAS. In conclusion, we reveal for the first time that HSP27 is a novel negative regulator of the cGAS-STING signaling pathway induced by PRV infection or poly(dA:dT) activation and demonstrate that HSP27 plays a crucial role in PRV infection.
650		4	\|a pseudorabies virus
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allfields	10.3390/v14091851 doi (DE-627)DOAJ084793309 (DE-599)DOAJ0f087a2d246740c3aa53afcf116a2258 DE-627 ger DE-627 rakwb eng QR1-502 Xiangrong Li verfasserin aut HSP27 Attenuates cGAS-Mediated IFN-β Signaling through Ubiquitination of cGAS and Promotes PRV Infection 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Pseudorabies (PR) is a domestic and wild animal infectious disease caused by the pseudorabies virus (PRV) and is one of the major infectious diseases that endanger the global swine industry. Studies have reported that PRV may achieve cross-species transmission from pigs to humans in recent years. Therefore, in-depth exploration of the relationship between PRV and host proteins is of great significance for elucidating the pathogenic mechanism of PRV and anti-PRV infection. Here, we report that heat shock protein 27 (HSP27) ubiquitinates and degrades cyclic GMP-AMP synthase (cGAS) and attenuates cGAS-mediated antiviral responses, thereby promoting PRV infection. Overexpression of HSP27 promoted PRV proliferation in vitro, while knockdown of HSP27 inhibited PRV infection. Importantly, we found that HSP27 inhibited PRV infection or poly(dA:dT)-activated IFN-β expression. Further studies found that HSP27 may inhibit cGAS-STING-mediated IFN-β expression through targeting cGAS. In addition, we found that HSP27 can suppress the expression of endogenous cGAS in different cells at both gene transcription and protein expression levels, and that HSP27 interacts with and ubiquitinates cGAS. In conclusion, we reveal for the first time that HSP27 is a novel negative regulator of the cGAS-STING signaling pathway induced by PRV infection or poly(dA:dT) activation and demonstrate that HSP27 plays a crucial role in PRV infection. pseudorabies virus heat shock protein 27 cyclic GMP-AMP synthase cGAS-STING signaling pathway ubiquitination Microbiology Jingying Xie verfasserin aut Dianyu Li verfasserin aut Hongshan Li verfasserin aut Yuhui Niu verfasserin aut Bei Wu verfasserin aut Yanmei Yang verfasserin aut Zhenfang Yan verfasserin aut Xiangbo Zhang verfasserin aut Lei Chen verfasserin aut Ruofei Feng verfasserin aut In Viruses MDPI AG, 2009 14(2022), 9, p 1851 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:14 year:2022 number:9, p 1851 https://doi.org/10.3390/v14091851 kostenfrei https://doaj.org/article/0f087a2d246740c3aa53afcf116a2258 kostenfrei https://www.mdpi.com/1999-4915/14/9/1851 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 9, p 1851
spelling	10.3390/v14091851 doi (DE-627)DOAJ084793309 (DE-599)DOAJ0f087a2d246740c3aa53afcf116a2258 DE-627 ger DE-627 rakwb eng QR1-502 Xiangrong Li verfasserin aut HSP27 Attenuates cGAS-Mediated IFN-β Signaling through Ubiquitination of cGAS and Promotes PRV Infection 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Pseudorabies (PR) is a domestic and wild animal infectious disease caused by the pseudorabies virus (PRV) and is one of the major infectious diseases that endanger the global swine industry. Studies have reported that PRV may achieve cross-species transmission from pigs to humans in recent years. Therefore, in-depth exploration of the relationship between PRV and host proteins is of great significance for elucidating the pathogenic mechanism of PRV and anti-PRV infection. Here, we report that heat shock protein 27 (HSP27) ubiquitinates and degrades cyclic GMP-AMP synthase (cGAS) and attenuates cGAS-mediated antiviral responses, thereby promoting PRV infection. Overexpression of HSP27 promoted PRV proliferation in vitro, while knockdown of HSP27 inhibited PRV infection. Importantly, we found that HSP27 inhibited PRV infection or poly(dA:dT)-activated IFN-β expression. Further studies found that HSP27 may inhibit cGAS-STING-mediated IFN-β expression through targeting cGAS. In addition, we found that HSP27 can suppress the expression of endogenous cGAS in different cells at both gene transcription and protein expression levels, and that HSP27 interacts with and ubiquitinates cGAS. In conclusion, we reveal for the first time that HSP27 is a novel negative regulator of the cGAS-STING signaling pathway induced by PRV infection or poly(dA:dT) activation and demonstrate that HSP27 plays a crucial role in PRV infection. pseudorabies virus heat shock protein 27 cyclic GMP-AMP synthase cGAS-STING signaling pathway ubiquitination Microbiology Jingying Xie verfasserin aut Dianyu Li verfasserin aut Hongshan Li verfasserin aut Yuhui Niu verfasserin aut Bei Wu verfasserin aut Yanmei Yang verfasserin aut Zhenfang Yan verfasserin aut Xiangbo Zhang verfasserin aut Lei Chen verfasserin aut Ruofei Feng verfasserin aut In Viruses MDPI AG, 2009 14(2022), 9, p 1851 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:14 year:2022 number:9, p 1851 https://doi.org/10.3390/v14091851 kostenfrei https://doaj.org/article/0f087a2d246740c3aa53afcf116a2258 kostenfrei https://www.mdpi.com/1999-4915/14/9/1851 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 9, p 1851
allfields_unstemmed	10.3390/v14091851 doi (DE-627)DOAJ084793309 (DE-599)DOAJ0f087a2d246740c3aa53afcf116a2258 DE-627 ger DE-627 rakwb eng QR1-502 Xiangrong Li verfasserin aut HSP27 Attenuates cGAS-Mediated IFN-β Signaling through Ubiquitination of cGAS and Promotes PRV Infection 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Pseudorabies (PR) is a domestic and wild animal infectious disease caused by the pseudorabies virus (PRV) and is one of the major infectious diseases that endanger the global swine industry. Studies have reported that PRV may achieve cross-species transmission from pigs to humans in recent years. Therefore, in-depth exploration of the relationship between PRV and host proteins is of great significance for elucidating the pathogenic mechanism of PRV and anti-PRV infection. Here, we report that heat shock protein 27 (HSP27) ubiquitinates and degrades cyclic GMP-AMP synthase (cGAS) and attenuates cGAS-mediated antiviral responses, thereby promoting PRV infection. Overexpression of HSP27 promoted PRV proliferation in vitro, while knockdown of HSP27 inhibited PRV infection. Importantly, we found that HSP27 inhibited PRV infection or poly(dA:dT)-activated IFN-β expression. Further studies found that HSP27 may inhibit cGAS-STING-mediated IFN-β expression through targeting cGAS. In addition, we found that HSP27 can suppress the expression of endogenous cGAS in different cells at both gene transcription and protein expression levels, and that HSP27 interacts with and ubiquitinates cGAS. In conclusion, we reveal for the first time that HSP27 is a novel negative regulator of the cGAS-STING signaling pathway induced by PRV infection or poly(dA:dT) activation and demonstrate that HSP27 plays a crucial role in PRV infection. pseudorabies virus heat shock protein 27 cyclic GMP-AMP synthase cGAS-STING signaling pathway ubiquitination Microbiology Jingying Xie verfasserin aut Dianyu Li verfasserin aut Hongshan Li verfasserin aut Yuhui Niu verfasserin aut Bei Wu verfasserin aut Yanmei Yang verfasserin aut Zhenfang Yan verfasserin aut Xiangbo Zhang verfasserin aut Lei Chen verfasserin aut Ruofei Feng verfasserin aut In Viruses MDPI AG, 2009 14(2022), 9, p 1851 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:14 year:2022 number:9, p 1851 https://doi.org/10.3390/v14091851 kostenfrei https://doaj.org/article/0f087a2d246740c3aa53afcf116a2258 kostenfrei https://www.mdpi.com/1999-4915/14/9/1851 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 9, p 1851
allfieldsGer	10.3390/v14091851 doi (DE-627)DOAJ084793309 (DE-599)DOAJ0f087a2d246740c3aa53afcf116a2258 DE-627 ger DE-627 rakwb eng QR1-502 Xiangrong Li verfasserin aut HSP27 Attenuates cGAS-Mediated IFN-β Signaling through Ubiquitination of cGAS and Promotes PRV Infection 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Pseudorabies (PR) is a domestic and wild animal infectious disease caused by the pseudorabies virus (PRV) and is one of the major infectious diseases that endanger the global swine industry. Studies have reported that PRV may achieve cross-species transmission from pigs to humans in recent years. Therefore, in-depth exploration of the relationship between PRV and host proteins is of great significance for elucidating the pathogenic mechanism of PRV and anti-PRV infection. Here, we report that heat shock protein 27 (HSP27) ubiquitinates and degrades cyclic GMP-AMP synthase (cGAS) and attenuates cGAS-mediated antiviral responses, thereby promoting PRV infection. Overexpression of HSP27 promoted PRV proliferation in vitro, while knockdown of HSP27 inhibited PRV infection. Importantly, we found that HSP27 inhibited PRV infection or poly(dA:dT)-activated IFN-β expression. Further studies found that HSP27 may inhibit cGAS-STING-mediated IFN-β expression through targeting cGAS. In addition, we found that HSP27 can suppress the expression of endogenous cGAS in different cells at both gene transcription and protein expression levels, and that HSP27 interacts with and ubiquitinates cGAS. In conclusion, we reveal for the first time that HSP27 is a novel negative regulator of the cGAS-STING signaling pathway induced by PRV infection or poly(dA:dT) activation and demonstrate that HSP27 plays a crucial role in PRV infection. pseudorabies virus heat shock protein 27 cyclic GMP-AMP synthase cGAS-STING signaling pathway ubiquitination Microbiology Jingying Xie verfasserin aut Dianyu Li verfasserin aut Hongshan Li verfasserin aut Yuhui Niu verfasserin aut Bei Wu verfasserin aut Yanmei Yang verfasserin aut Zhenfang Yan verfasserin aut Xiangbo Zhang verfasserin aut Lei Chen verfasserin aut Ruofei Feng verfasserin aut In Viruses MDPI AG, 2009 14(2022), 9, p 1851 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:14 year:2022 number:9, p 1851 https://doi.org/10.3390/v14091851 kostenfrei https://doaj.org/article/0f087a2d246740c3aa53afcf116a2258 kostenfrei https://www.mdpi.com/1999-4915/14/9/1851 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 9, p 1851
allfieldsSound	10.3390/v14091851 doi (DE-627)DOAJ084793309 (DE-599)DOAJ0f087a2d246740c3aa53afcf116a2258 DE-627 ger DE-627 rakwb eng QR1-502 Xiangrong Li verfasserin aut HSP27 Attenuates cGAS-Mediated IFN-β Signaling through Ubiquitination of cGAS and Promotes PRV Infection 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Pseudorabies (PR) is a domestic and wild animal infectious disease caused by the pseudorabies virus (PRV) and is one of the major infectious diseases that endanger the global swine industry. Studies have reported that PRV may achieve cross-species transmission from pigs to humans in recent years. Therefore, in-depth exploration of the relationship between PRV and host proteins is of great significance for elucidating the pathogenic mechanism of PRV and anti-PRV infection. Here, we report that heat shock protein 27 (HSP27) ubiquitinates and degrades cyclic GMP-AMP synthase (cGAS) and attenuates cGAS-mediated antiviral responses, thereby promoting PRV infection. Overexpression of HSP27 promoted PRV proliferation in vitro, while knockdown of HSP27 inhibited PRV infection. Importantly, we found that HSP27 inhibited PRV infection or poly(dA:dT)-activated IFN-β expression. Further studies found that HSP27 may inhibit cGAS-STING-mediated IFN-β expression through targeting cGAS. In addition, we found that HSP27 can suppress the expression of endogenous cGAS in different cells at both gene transcription and protein expression levels, and that HSP27 interacts with and ubiquitinates cGAS. In conclusion, we reveal for the first time that HSP27 is a novel negative regulator of the cGAS-STING signaling pathway induced by PRV infection or poly(dA:dT) activation and demonstrate that HSP27 plays a crucial role in PRV infection. pseudorabies virus heat shock protein 27 cyclic GMP-AMP synthase cGAS-STING signaling pathway ubiquitination Microbiology Jingying Xie verfasserin aut Dianyu Li verfasserin aut Hongshan Li verfasserin aut Yuhui Niu verfasserin aut Bei Wu verfasserin aut Yanmei Yang verfasserin aut Zhenfang Yan verfasserin aut Xiangbo Zhang verfasserin aut Lei Chen verfasserin aut Ruofei Feng verfasserin aut In Viruses MDPI AG, 2009 14(2022), 9, p 1851 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:14 year:2022 number:9, p 1851 https://doi.org/10.3390/v14091851 kostenfrei https://doaj.org/article/0f087a2d246740c3aa53afcf116a2258 kostenfrei https://www.mdpi.com/1999-4915/14/9/1851 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 9, p 1851
language	English
source	In Viruses 14(2022), 9, p 1851 volume:14 year:2022 number:9, p 1851
sourceStr	In Viruses 14(2022), 9, p 1851 volume:14 year:2022 number:9, p 1851
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	pseudorabies virus heat shock protein 27 cyclic GMP-AMP synthase cGAS-STING signaling pathway ubiquitination Microbiology
isfreeaccess_bool	true
container_title	Viruses
authorswithroles_txt_mv	Xiangrong Li @@aut@@ Jingying Xie @@aut@@ Dianyu Li @@aut@@ Hongshan Li @@aut@@ Yuhui Niu @@aut@@ Bei Wu @@aut@@ Yanmei Yang @@aut@@ Zhenfang Yan @@aut@@ Xiangbo Zhang @@aut@@ Lei Chen @@aut@@ Ruofei Feng @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	609775871
id	DOAJ084793309
language_de	englisch
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callnumber-first	Q - Science
author	Xiangrong Li
spellingShingle	Xiangrong Li misc QR1-502 misc pseudorabies virus misc heat shock protein 27 misc cyclic GMP-AMP synthase misc cGAS-STING signaling pathway misc ubiquitination misc Microbiology HSP27 Attenuates cGAS-Mediated IFN-β Signaling through Ubiquitination of cGAS and Promotes PRV Infection
authorStr	Xiangrong Li
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topic_title	QR1-502 HSP27 Attenuates cGAS-Mediated IFN-β Signaling through Ubiquitination of cGAS and Promotes PRV Infection pseudorabies virus heat shock protein 27 cyclic GMP-AMP synthase cGAS-STING signaling pathway ubiquitination
topic	misc QR1-502 misc pseudorabies virus misc heat shock protein 27 misc cyclic GMP-AMP synthase misc cGAS-STING signaling pathway misc ubiquitination misc Microbiology
topic_unstemmed	misc QR1-502 misc pseudorabies virus misc heat shock protein 27 misc cyclic GMP-AMP synthase misc cGAS-STING signaling pathway misc ubiquitination misc Microbiology
topic_browse	misc QR1-502 misc pseudorabies virus misc heat shock protein 27 misc cyclic GMP-AMP synthase misc cGAS-STING signaling pathway misc ubiquitination misc Microbiology
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title	HSP27 Attenuates cGAS-Mediated IFN-β Signaling through Ubiquitination of cGAS and Promotes PRV Infection
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title_full	HSP27 Attenuates cGAS-Mediated IFN-β Signaling through Ubiquitination of cGAS and Promotes PRV Infection
author_sort	Xiangrong Li
journal	Viruses
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author_browse	Xiangrong Li Jingying Xie Dianyu Li Hongshan Li Yuhui Niu Bei Wu Yanmei Yang Zhenfang Yan Xiangbo Zhang Lei Chen Ruofei Feng
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author-letter	Xiangrong Li
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title_sort	hsp27 attenuates cgas-mediated ifn-β signaling through ubiquitination of cgas and promotes prv infection
callnumber	QR1-502
title_auth	HSP27 Attenuates cGAS-Mediated IFN-β Signaling through Ubiquitination of cGAS and Promotes PRV Infection
abstract	Pseudorabies (PR) is a domestic and wild animal infectious disease caused by the pseudorabies virus (PRV) and is one of the major infectious diseases that endanger the global swine industry. Studies have reported that PRV may achieve cross-species transmission from pigs to humans in recent years. Therefore, in-depth exploration of the relationship between PRV and host proteins is of great significance for elucidating the pathogenic mechanism of PRV and anti-PRV infection. Here, we report that heat shock protein 27 (HSP27) ubiquitinates and degrades cyclic GMP-AMP synthase (cGAS) and attenuates cGAS-mediated antiviral responses, thereby promoting PRV infection. Overexpression of HSP27 promoted PRV proliferation in vitro, while knockdown of HSP27 inhibited PRV infection. Importantly, we found that HSP27 inhibited PRV infection or poly(dA:dT)-activated IFN-β expression. Further studies found that HSP27 may inhibit cGAS-STING-mediated IFN-β expression through targeting cGAS. In addition, we found that HSP27 can suppress the expression of endogenous cGAS in different cells at both gene transcription and protein expression levels, and that HSP27 interacts with and ubiquitinates cGAS. In conclusion, we reveal for the first time that HSP27 is a novel negative regulator of the cGAS-STING signaling pathway induced by PRV infection or poly(dA:dT) activation and demonstrate that HSP27 plays a crucial role in PRV infection.
abstractGer	Pseudorabies (PR) is a domestic and wild animal infectious disease caused by the pseudorabies virus (PRV) and is one of the major infectious diseases that endanger the global swine industry. Studies have reported that PRV may achieve cross-species transmission from pigs to humans in recent years. Therefore, in-depth exploration of the relationship between PRV and host proteins is of great significance for elucidating the pathogenic mechanism of PRV and anti-PRV infection. Here, we report that heat shock protein 27 (HSP27) ubiquitinates and degrades cyclic GMP-AMP synthase (cGAS) and attenuates cGAS-mediated antiviral responses, thereby promoting PRV infection. Overexpression of HSP27 promoted PRV proliferation in vitro, while knockdown of HSP27 inhibited PRV infection. Importantly, we found that HSP27 inhibited PRV infection or poly(dA:dT)-activated IFN-β expression. Further studies found that HSP27 may inhibit cGAS-STING-mediated IFN-β expression through targeting cGAS. In addition, we found that HSP27 can suppress the expression of endogenous cGAS in different cells at both gene transcription and protein expression levels, and that HSP27 interacts with and ubiquitinates cGAS. In conclusion, we reveal for the first time that HSP27 is a novel negative regulator of the cGAS-STING signaling pathway induced by PRV infection or poly(dA:dT) activation and demonstrate that HSP27 plays a crucial role in PRV infection.
abstract_unstemmed	Pseudorabies (PR) is a domestic and wild animal infectious disease caused by the pseudorabies virus (PRV) and is one of the major infectious diseases that endanger the global swine industry. Studies have reported that PRV may achieve cross-species transmission from pigs to humans in recent years. Therefore, in-depth exploration of the relationship between PRV and host proteins is of great significance for elucidating the pathogenic mechanism of PRV and anti-PRV infection. Here, we report that heat shock protein 27 (HSP27) ubiquitinates and degrades cyclic GMP-AMP synthase (cGAS) and attenuates cGAS-mediated antiviral responses, thereby promoting PRV infection. Overexpression of HSP27 promoted PRV proliferation in vitro, while knockdown of HSP27 inhibited PRV infection. Importantly, we found that HSP27 inhibited PRV infection or poly(dA:dT)-activated IFN-β expression. Further studies found that HSP27 may inhibit cGAS-STING-mediated IFN-β expression through targeting cGAS. In addition, we found that HSP27 can suppress the expression of endogenous cGAS in different cells at both gene transcription and protein expression levels, and that HSP27 interacts with and ubiquitinates cGAS. In conclusion, we reveal for the first time that HSP27 is a novel negative regulator of the cGAS-STING signaling pathway induced by PRV infection or poly(dA:dT) activation and demonstrate that HSP27 plays a crucial role in PRV infection.
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container_issue	9, p 1851
title_short	HSP27 Attenuates cGAS-Mediated IFN-β Signaling through Ubiquitination of cGAS and Promotes PRV Infection
url	https://doi.org/10.3390/v14091851 https://doaj.org/article/0f087a2d246740c3aa53afcf116a2258 https://www.mdpi.com/1999-4915/14/9/1851 https://doaj.org/toc/1999-4915
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up_date	2024-07-04T00:34:07.466Z
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