ROS Induced by <i<Streptococcus agalactiae</i< Activate Inflammatory Responses via the TNF-α/NF-κB Signaling Pathway in Golden Pompano <i<Trachinotus ovatus</i< (Linnaeus, 1758)

<i<Streptococcus agalactiae</i< is common pathogenic bacteria in aquaculture and can cause mass mortality after fish infection. This study aimed to investigate the effects of <i<S. agalactiae</i< infection on the immune and antioxidant regulatory mechanisms of golden pompano (<i<Trachinotus ovatus</i<). Serum and liver samples were obtained at 0, 6, 12, 24, 48, 96, and 120 h after golden pompano infection with <i<S. agalactiae</i< for enzyme activity and gene expression analyses. After infection with <i<S. agalactiae</i<, the content of reactive oxygen species (ROS) in serum was significantly increased (<i<p</i< < 0.05). Serum levels of glucose (GLU), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and malondialdehyde (MDA) increased and then decreased (<i<p</i< < 0.05), reaching a maximum at 6 h. Serum antioxidant enzyme (LZM) activity increased significantly (<i<p</i< < 0.05) and reached a maximum at 120 h. In addition, the mRNA expression levels of antioxidant genes (<i<SOD</i<, <i<CAT</i<, and <i<GPx</i<) in the liver increased and then decreased, reaching the maximum at 24 h, 48 h, and 24 h, respectively. During the experimental period, the mRNA expression levels of NF-κB-related genes of the inflammatory signaling pathway inhibitory κB (<i<IκB</i<) showed an overall decreasing trend (<i<p</i< < 0.05) and the lowest expression at 120 h, whereas the mRNA expression levels of tumor necrosis factor α (<i<TNF-α</i<), interleukin-1β (<i<IL-1β</i<), IκB kinase (<i<IKK</i<), and nuclear factor NF-κB increased significantly (<i<p</i< < 0.05) and the highest expression was at 120 h. In conclusion, these results showed that <i<S. agalactiae</i< could activate internal regulatory signaling in the liver of golden pompano to induce defense and immune responses. This study is expected to lay a foundation to develop the healthy aquaculture of golden pompano and promote a more comprehensive understanding of its disease resistance mechanisms. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Jie Gao [verfasserIn] Mingjian Liu [verfasserIn] Huayang Guo [verfasserIn] Kecheng Zhu [verfasserIn] Bo Liu [verfasserIn] Baosuo Liu [verfasserIn] Nan Zhang [verfasserIn] Dianchang Zhang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	pathogenic bacterial infection histopathology immune-related genes inflammatory response

Übergeordnetes Werk:	In: Antioxidants - MDPI AG, 2013, 11(2022), 9, p 1809
Übergeordnetes Werk:	volume:11 ; year:2022 ; number:9, p 1809

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/antiox11091809

Katalog-ID:	DOAJ084836423

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245	1	0	\|a ROS Induced by <i<Streptococcus agalactiae</i< Activate Inflammatory Responses via the TNF-α/NF-κB Signaling Pathway in Golden Pompano <i<Trachinotus ovatus</i< (Linnaeus, 1758)
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allfields	10.3390/antiox11091809 doi (DE-627)DOAJ084836423 (DE-599)DOAJ9c41f15bf1124080a85157ee7aa175d8 DE-627 ger DE-627 rakwb eng RM1-950 Jie Gao verfasserin aut ROS Induced by <i<Streptococcus agalactiae</i< Activate Inflammatory Responses via the TNF-α/NF-κB Signaling Pathway in Golden Pompano <i<Trachinotus ovatus</i< (Linnaeus, 1758) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Streptococcus agalactiae</i< is common pathogenic bacteria in aquaculture and can cause mass mortality after fish infection. This study aimed to investigate the effects of <i<S. agalactiae</i< infection on the immune and antioxidant regulatory mechanisms of golden pompano (<i<Trachinotus ovatus</i<). Serum and liver samples were obtained at 0, 6, 12, 24, 48, 96, and 120 h after golden pompano infection with <i<S. agalactiae</i< for enzyme activity and gene expression analyses. After infection with <i<S. agalactiae</i<, the content of reactive oxygen species (ROS) in serum was significantly increased (<i<p</i< < 0.05). Serum levels of glucose (GLU), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and malondialdehyde (MDA) increased and then decreased (<i<p</i< < 0.05), reaching a maximum at 6 h. Serum antioxidant enzyme (LZM) activity increased significantly (<i<p</i< < 0.05) and reached a maximum at 120 h. In addition, the mRNA expression levels of antioxidant genes (<i<SOD</i<, <i<CAT</i<, and <i<GPx</i<) in the liver increased and then decreased, reaching the maximum at 24 h, 48 h, and 24 h, respectively. During the experimental period, the mRNA expression levels of NF-κB-related genes of the inflammatory signaling pathway inhibitory κB (<i<IκB</i<) showed an overall decreasing trend (<i<p</i< < 0.05) and the lowest expression at 120 h, whereas the mRNA expression levels of tumor necrosis factor α (<i<TNF-α</i<), interleukin-1β (<i<IL-1β</i<), IκB kinase (<i<IKK</i<), and nuclear factor NF-κB increased significantly (<i<p</i< < 0.05) and the highest expression was at 120 h. In conclusion, these results showed that <i<S. agalactiae</i< could activate internal regulatory signaling in the liver of golden pompano to induce defense and immune responses. This study is expected to lay a foundation to develop the healthy aquaculture of golden pompano and promote a more comprehensive understanding of its disease resistance mechanisms. <i<Trachinotus ovatus</i< pathogenic bacterial infection histopathology immune-related genes inflammatory response Therapeutics. Pharmacology Mingjian Liu verfasserin aut Huayang Guo verfasserin aut Kecheng Zhu verfasserin aut Bo Liu verfasserin aut Baosuo Liu verfasserin aut Nan Zhang verfasserin aut Dianchang Zhang verfasserin aut In Antioxidants MDPI AG, 2013 11(2022), 9, p 1809 (DE-627)737287578 (DE-600)2704216-9 20763921 nnns volume:11 year:2022 number:9, p 1809 https://doi.org/10.3390/antiox11091809 kostenfrei https://doaj.org/article/9c41f15bf1124080a85157ee7aa175d8 kostenfrei https://www.mdpi.com/2076-3921/11/9/1809 kostenfrei https://doaj.org/toc/2076-3921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 9, p 1809
spelling	10.3390/antiox11091809 doi (DE-627)DOAJ084836423 (DE-599)DOAJ9c41f15bf1124080a85157ee7aa175d8 DE-627 ger DE-627 rakwb eng RM1-950 Jie Gao verfasserin aut ROS Induced by <i<Streptococcus agalactiae</i< Activate Inflammatory Responses via the TNF-α/NF-κB Signaling Pathway in Golden Pompano <i<Trachinotus ovatus</i< (Linnaeus, 1758) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Streptococcus agalactiae</i< is common pathogenic bacteria in aquaculture and can cause mass mortality after fish infection. This study aimed to investigate the effects of <i<S. agalactiae</i< infection on the immune and antioxidant regulatory mechanisms of golden pompano (<i<Trachinotus ovatus</i<). Serum and liver samples were obtained at 0, 6, 12, 24, 48, 96, and 120 h after golden pompano infection with <i<S. agalactiae</i< for enzyme activity and gene expression analyses. After infection with <i<S. agalactiae</i<, the content of reactive oxygen species (ROS) in serum was significantly increased (<i<p</i< < 0.05). Serum levels of glucose (GLU), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and malondialdehyde (MDA) increased and then decreased (<i<p</i< < 0.05), reaching a maximum at 6 h. Serum antioxidant enzyme (LZM) activity increased significantly (<i<p</i< < 0.05) and reached a maximum at 120 h. In addition, the mRNA expression levels of antioxidant genes (<i<SOD</i<, <i<CAT</i<, and <i<GPx</i<) in the liver increased and then decreased, reaching the maximum at 24 h, 48 h, and 24 h, respectively. During the experimental period, the mRNA expression levels of NF-κB-related genes of the inflammatory signaling pathway inhibitory κB (<i<IκB</i<) showed an overall decreasing trend (<i<p</i< < 0.05) and the lowest expression at 120 h, whereas the mRNA expression levels of tumor necrosis factor α (<i<TNF-α</i<), interleukin-1β (<i<IL-1β</i<), IκB kinase (<i<IKK</i<), and nuclear factor NF-κB increased significantly (<i<p</i< < 0.05) and the highest expression was at 120 h. In conclusion, these results showed that <i<S. agalactiae</i< could activate internal regulatory signaling in the liver of golden pompano to induce defense and immune responses. This study is expected to lay a foundation to develop the healthy aquaculture of golden pompano and promote a more comprehensive understanding of its disease resistance mechanisms. <i<Trachinotus ovatus</i< pathogenic bacterial infection histopathology immune-related genes inflammatory response Therapeutics. Pharmacology Mingjian Liu verfasserin aut Huayang Guo verfasserin aut Kecheng Zhu verfasserin aut Bo Liu verfasserin aut Baosuo Liu verfasserin aut Nan Zhang verfasserin aut Dianchang Zhang verfasserin aut In Antioxidants MDPI AG, 2013 11(2022), 9, p 1809 (DE-627)737287578 (DE-600)2704216-9 20763921 nnns volume:11 year:2022 number:9, p 1809 https://doi.org/10.3390/antiox11091809 kostenfrei https://doaj.org/article/9c41f15bf1124080a85157ee7aa175d8 kostenfrei https://www.mdpi.com/2076-3921/11/9/1809 kostenfrei https://doaj.org/toc/2076-3921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 9, p 1809
allfields_unstemmed	10.3390/antiox11091809 doi (DE-627)DOAJ084836423 (DE-599)DOAJ9c41f15bf1124080a85157ee7aa175d8 DE-627 ger DE-627 rakwb eng RM1-950 Jie Gao verfasserin aut ROS Induced by <i<Streptococcus agalactiae</i< Activate Inflammatory Responses via the TNF-α/NF-κB Signaling Pathway in Golden Pompano <i<Trachinotus ovatus</i< (Linnaeus, 1758) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Streptococcus agalactiae</i< is common pathogenic bacteria in aquaculture and can cause mass mortality after fish infection. This study aimed to investigate the effects of <i<S. agalactiae</i< infection on the immune and antioxidant regulatory mechanisms of golden pompano (<i<Trachinotus ovatus</i<). Serum and liver samples were obtained at 0, 6, 12, 24, 48, 96, and 120 h after golden pompano infection with <i<S. agalactiae</i< for enzyme activity and gene expression analyses. After infection with <i<S. agalactiae</i<, the content of reactive oxygen species (ROS) in serum was significantly increased (<i<p</i< < 0.05). Serum levels of glucose (GLU), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and malondialdehyde (MDA) increased and then decreased (<i<p</i< < 0.05), reaching a maximum at 6 h. Serum antioxidant enzyme (LZM) activity increased significantly (<i<p</i< < 0.05) and reached a maximum at 120 h. In addition, the mRNA expression levels of antioxidant genes (<i<SOD</i<, <i<CAT</i<, and <i<GPx</i<) in the liver increased and then decreased, reaching the maximum at 24 h, 48 h, and 24 h, respectively. During the experimental period, the mRNA expression levels of NF-κB-related genes of the inflammatory signaling pathway inhibitory κB (<i<IκB</i<) showed an overall decreasing trend (<i<p</i< < 0.05) and the lowest expression at 120 h, whereas the mRNA expression levels of tumor necrosis factor α (<i<TNF-α</i<), interleukin-1β (<i<IL-1β</i<), IκB kinase (<i<IKK</i<), and nuclear factor NF-κB increased significantly (<i<p</i< < 0.05) and the highest expression was at 120 h. In conclusion, these results showed that <i<S. agalactiae</i< could activate internal regulatory signaling in the liver of golden pompano to induce defense and immune responses. This study is expected to lay a foundation to develop the healthy aquaculture of golden pompano and promote a more comprehensive understanding of its disease resistance mechanisms. <i<Trachinotus ovatus</i< pathogenic bacterial infection histopathology immune-related genes inflammatory response Therapeutics. Pharmacology Mingjian Liu verfasserin aut Huayang Guo verfasserin aut Kecheng Zhu verfasserin aut Bo Liu verfasserin aut Baosuo Liu verfasserin aut Nan Zhang verfasserin aut Dianchang Zhang verfasserin aut In Antioxidants MDPI AG, 2013 11(2022), 9, p 1809 (DE-627)737287578 (DE-600)2704216-9 20763921 nnns volume:11 year:2022 number:9, p 1809 https://doi.org/10.3390/antiox11091809 kostenfrei https://doaj.org/article/9c41f15bf1124080a85157ee7aa175d8 kostenfrei https://www.mdpi.com/2076-3921/11/9/1809 kostenfrei https://doaj.org/toc/2076-3921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 9, p 1809
allfieldsGer	10.3390/antiox11091809 doi (DE-627)DOAJ084836423 (DE-599)DOAJ9c41f15bf1124080a85157ee7aa175d8 DE-627 ger DE-627 rakwb eng RM1-950 Jie Gao verfasserin aut ROS Induced by <i<Streptococcus agalactiae</i< Activate Inflammatory Responses via the TNF-α/NF-κB Signaling Pathway in Golden Pompano <i<Trachinotus ovatus</i< (Linnaeus, 1758) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Streptococcus agalactiae</i< is common pathogenic bacteria in aquaculture and can cause mass mortality after fish infection. This study aimed to investigate the effects of <i<S. agalactiae</i< infection on the immune and antioxidant regulatory mechanisms of golden pompano (<i<Trachinotus ovatus</i<). Serum and liver samples were obtained at 0, 6, 12, 24, 48, 96, and 120 h after golden pompano infection with <i<S. agalactiae</i< for enzyme activity and gene expression analyses. After infection with <i<S. agalactiae</i<, the content of reactive oxygen species (ROS) in serum was significantly increased (<i<p</i< < 0.05). Serum levels of glucose (GLU), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and malondialdehyde (MDA) increased and then decreased (<i<p</i< < 0.05), reaching a maximum at 6 h. Serum antioxidant enzyme (LZM) activity increased significantly (<i<p</i< < 0.05) and reached a maximum at 120 h. In addition, the mRNA expression levels of antioxidant genes (<i<SOD</i<, <i<CAT</i<, and <i<GPx</i<) in the liver increased and then decreased, reaching the maximum at 24 h, 48 h, and 24 h, respectively. During the experimental period, the mRNA expression levels of NF-κB-related genes of the inflammatory signaling pathway inhibitory κB (<i<IκB</i<) showed an overall decreasing trend (<i<p</i< < 0.05) and the lowest expression at 120 h, whereas the mRNA expression levels of tumor necrosis factor α (<i<TNF-α</i<), interleukin-1β (<i<IL-1β</i<), IκB kinase (<i<IKK</i<), and nuclear factor NF-κB increased significantly (<i<p</i< < 0.05) and the highest expression was at 120 h. In conclusion, these results showed that <i<S. agalactiae</i< could activate internal regulatory signaling in the liver of golden pompano to induce defense and immune responses. This study is expected to lay a foundation to develop the healthy aquaculture of golden pompano and promote a more comprehensive understanding of its disease resistance mechanisms. <i<Trachinotus ovatus</i< pathogenic bacterial infection histopathology immune-related genes inflammatory response Therapeutics. Pharmacology Mingjian Liu verfasserin aut Huayang Guo verfasserin aut Kecheng Zhu verfasserin aut Bo Liu verfasserin aut Baosuo Liu verfasserin aut Nan Zhang verfasserin aut Dianchang Zhang verfasserin aut In Antioxidants MDPI AG, 2013 11(2022), 9, p 1809 (DE-627)737287578 (DE-600)2704216-9 20763921 nnns volume:11 year:2022 number:9, p 1809 https://doi.org/10.3390/antiox11091809 kostenfrei https://doaj.org/article/9c41f15bf1124080a85157ee7aa175d8 kostenfrei https://www.mdpi.com/2076-3921/11/9/1809 kostenfrei https://doaj.org/toc/2076-3921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 9, p 1809
allfieldsSound	10.3390/antiox11091809 doi (DE-627)DOAJ084836423 (DE-599)DOAJ9c41f15bf1124080a85157ee7aa175d8 DE-627 ger DE-627 rakwb eng RM1-950 Jie Gao verfasserin aut ROS Induced by <i<Streptococcus agalactiae</i< Activate Inflammatory Responses via the TNF-α/NF-κB Signaling Pathway in Golden Pompano <i<Trachinotus ovatus</i< (Linnaeus, 1758) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Streptococcus agalactiae</i< is common pathogenic bacteria in aquaculture and can cause mass mortality after fish infection. This study aimed to investigate the effects of <i<S. agalactiae</i< infection on the immune and antioxidant regulatory mechanisms of golden pompano (<i<Trachinotus ovatus</i<). Serum and liver samples were obtained at 0, 6, 12, 24, 48, 96, and 120 h after golden pompano infection with <i<S. agalactiae</i< for enzyme activity and gene expression analyses. After infection with <i<S. agalactiae</i<, the content of reactive oxygen species (ROS) in serum was significantly increased (<i<p</i< < 0.05). Serum levels of glucose (GLU), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and malondialdehyde (MDA) increased and then decreased (<i<p</i< < 0.05), reaching a maximum at 6 h. Serum antioxidant enzyme (LZM) activity increased significantly (<i<p</i< < 0.05) and reached a maximum at 120 h. In addition, the mRNA expression levels of antioxidant genes (<i<SOD</i<, <i<CAT</i<, and <i<GPx</i<) in the liver increased and then decreased, reaching the maximum at 24 h, 48 h, and 24 h, respectively. During the experimental period, the mRNA expression levels of NF-κB-related genes of the inflammatory signaling pathway inhibitory κB (<i<IκB</i<) showed an overall decreasing trend (<i<p</i< < 0.05) and the lowest expression at 120 h, whereas the mRNA expression levels of tumor necrosis factor α (<i<TNF-α</i<), interleukin-1β (<i<IL-1β</i<), IκB kinase (<i<IKK</i<), and nuclear factor NF-κB increased significantly (<i<p</i< < 0.05) and the highest expression was at 120 h. In conclusion, these results showed that <i<S. agalactiae</i< could activate internal regulatory signaling in the liver of golden pompano to induce defense and immune responses. This study is expected to lay a foundation to develop the healthy aquaculture of golden pompano and promote a more comprehensive understanding of its disease resistance mechanisms. <i<Trachinotus ovatus</i< pathogenic bacterial infection histopathology immune-related genes inflammatory response Therapeutics. Pharmacology Mingjian Liu verfasserin aut Huayang Guo verfasserin aut Kecheng Zhu verfasserin aut Bo Liu verfasserin aut Baosuo Liu verfasserin aut Nan Zhang verfasserin aut Dianchang Zhang verfasserin aut In Antioxidants MDPI AG, 2013 11(2022), 9, p 1809 (DE-627)737287578 (DE-600)2704216-9 20763921 nnns volume:11 year:2022 number:9, p 1809 https://doi.org/10.3390/antiox11091809 kostenfrei https://doaj.org/article/9c41f15bf1124080a85157ee7aa175d8 kostenfrei https://www.mdpi.com/2076-3921/11/9/1809 kostenfrei https://doaj.org/toc/2076-3921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 9, p 1809
language	English
source	In Antioxidants 11(2022), 9, p 1809 volume:11 year:2022 number:9, p 1809
sourceStr	In Antioxidants 11(2022), 9, p 1809 volume:11 year:2022 number:9, p 1809
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<Trachinotus ovatus</i< pathogenic bacterial infection histopathology immune-related genes inflammatory response Therapeutics. Pharmacology
isfreeaccess_bool	true
container_title	Antioxidants
authorswithroles_txt_mv	Jie Gao @@aut@@ Mingjian Liu @@aut@@ Huayang Guo @@aut@@ Kecheng Zhu @@aut@@ Bo Liu @@aut@@ Baosuo Liu @@aut@@ Nan Zhang @@aut@@ Dianchang Zhang @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	737287578
id	DOAJ084836423
language_de	englisch
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callnumber-first	R - Medicine
author	Jie Gao
spellingShingle	Jie Gao misc RM1-950 misc <i<Trachinotus ovatus</i< misc pathogenic bacterial infection misc histopathology misc immune-related genes misc inflammatory response misc Therapeutics. Pharmacology ROS Induced by <i<Streptococcus agalactiae</i< Activate Inflammatory Responses via the TNF-α/NF-κB Signaling Pathway in Golden Pompano <i<Trachinotus ovatus</i< (Linnaeus, 1758)
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topic_title	RM1-950 ROS Induced by <i<Streptococcus agalactiae</i< Activate Inflammatory Responses via the TNF-α/NF-κB Signaling Pathway in Golden Pompano <i<Trachinotus ovatus</i< (Linnaeus, 1758) <i<Trachinotus ovatus</i< pathogenic bacterial infection histopathology immune-related genes inflammatory response
topic	misc RM1-950 misc <i<Trachinotus ovatus</i< misc pathogenic bacterial infection misc histopathology misc immune-related genes misc inflammatory response misc Therapeutics. Pharmacology
topic_unstemmed	misc RM1-950 misc <i<Trachinotus ovatus</i< misc pathogenic bacterial infection misc histopathology misc immune-related genes misc inflammatory response misc Therapeutics. Pharmacology
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title	ROS Induced by <i<Streptococcus agalactiae</i< Activate Inflammatory Responses via the TNF-α/NF-κB Signaling Pathway in Golden Pompano <i<Trachinotus ovatus</i< (Linnaeus, 1758)
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title_full	ROS Induced by <i<Streptococcus agalactiae</i< Activate Inflammatory Responses via the TNF-α/NF-κB Signaling Pathway in Golden Pompano <i<Trachinotus ovatus</i< (Linnaeus, 1758)
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author_browse	Jie Gao Mingjian Liu Huayang Guo Kecheng Zhu Bo Liu Baosuo Liu Nan Zhang Dianchang Zhang
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class	RM1-950
format_se	Elektronische Aufsätze
author-letter	Jie Gao
doi_str_mv	10.3390/antiox11091809
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title_sort	ros induced by <i<streptococcus agalactiae</i< activate inflammatory responses via the tnf-α/nf-κb signaling pathway in golden pompano <i<trachinotus ovatus</i< (linnaeus, 1758)
callnumber	RM1-950
title_auth	ROS Induced by <i<Streptococcus agalactiae</i< Activate Inflammatory Responses via the TNF-α/NF-κB Signaling Pathway in Golden Pompano <i<Trachinotus ovatus</i< (Linnaeus, 1758)
abstract	<i<Streptococcus agalactiae</i< is common pathogenic bacteria in aquaculture and can cause mass mortality after fish infection. This study aimed to investigate the effects of <i<S. agalactiae</i< infection on the immune and antioxidant regulatory mechanisms of golden pompano (<i<Trachinotus ovatus</i<). Serum and liver samples were obtained at 0, 6, 12, 24, 48, 96, and 120 h after golden pompano infection with <i<S. agalactiae</i< for enzyme activity and gene expression analyses. After infection with <i<S. agalactiae</i<, the content of reactive oxygen species (ROS) in serum was significantly increased (<i<p</i< < 0.05). Serum levels of glucose (GLU), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and malondialdehyde (MDA) increased and then decreased (<i<p</i< < 0.05), reaching a maximum at 6 h. Serum antioxidant enzyme (LZM) activity increased significantly (<i<p</i< < 0.05) and reached a maximum at 120 h. In addition, the mRNA expression levels of antioxidant genes (<i<SOD</i<, <i<CAT</i<, and <i<GPx</i<) in the liver increased and then decreased, reaching the maximum at 24 h, 48 h, and 24 h, respectively. During the experimental period, the mRNA expression levels of NF-κB-related genes of the inflammatory signaling pathway inhibitory κB (<i<IκB</i<) showed an overall decreasing trend (<i<p</i< < 0.05) and the lowest expression at 120 h, whereas the mRNA expression levels of tumor necrosis factor α (<i<TNF-α</i<), interleukin-1β (<i<IL-1β</i<), IκB kinase (<i<IKK</i<), and nuclear factor NF-κB increased significantly (<i<p</i< < 0.05) and the highest expression was at 120 h. In conclusion, these results showed that <i<S. agalactiae</i< could activate internal regulatory signaling in the liver of golden pompano to induce defense and immune responses. This study is expected to lay a foundation to develop the healthy aquaculture of golden pompano and promote a more comprehensive understanding of its disease resistance mechanisms.
abstractGer	<i<Streptococcus agalactiae</i< is common pathogenic bacteria in aquaculture and can cause mass mortality after fish infection. This study aimed to investigate the effects of <i<S. agalactiae</i< infection on the immune and antioxidant regulatory mechanisms of golden pompano (<i<Trachinotus ovatus</i<). Serum and liver samples were obtained at 0, 6, 12, 24, 48, 96, and 120 h after golden pompano infection with <i<S. agalactiae</i< for enzyme activity and gene expression analyses. After infection with <i<S. agalactiae</i<, the content of reactive oxygen species (ROS) in serum was significantly increased (<i<p</i< < 0.05). Serum levels of glucose (GLU), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and malondialdehyde (MDA) increased and then decreased (<i<p</i< < 0.05), reaching a maximum at 6 h. Serum antioxidant enzyme (LZM) activity increased significantly (<i<p</i< < 0.05) and reached a maximum at 120 h. In addition, the mRNA expression levels of antioxidant genes (<i<SOD</i<, <i<CAT</i<, and <i<GPx</i<) in the liver increased and then decreased, reaching the maximum at 24 h, 48 h, and 24 h, respectively. During the experimental period, the mRNA expression levels of NF-κB-related genes of the inflammatory signaling pathway inhibitory κB (<i<IκB</i<) showed an overall decreasing trend (<i<p</i< < 0.05) and the lowest expression at 120 h, whereas the mRNA expression levels of tumor necrosis factor α (<i<TNF-α</i<), interleukin-1β (<i<IL-1β</i<), IκB kinase (<i<IKK</i<), and nuclear factor NF-κB increased significantly (<i<p</i< < 0.05) and the highest expression was at 120 h. In conclusion, these results showed that <i<S. agalactiae</i< could activate internal regulatory signaling in the liver of golden pompano to induce defense and immune responses. This study is expected to lay a foundation to develop the healthy aquaculture of golden pompano and promote a more comprehensive understanding of its disease resistance mechanisms.
abstract_unstemmed	<i<Streptococcus agalactiae</i< is common pathogenic bacteria in aquaculture and can cause mass mortality after fish infection. This study aimed to investigate the effects of <i<S. agalactiae</i< infection on the immune and antioxidant regulatory mechanisms of golden pompano (<i<Trachinotus ovatus</i<). Serum and liver samples were obtained at 0, 6, 12, 24, 48, 96, and 120 h after golden pompano infection with <i<S. agalactiae</i< for enzyme activity and gene expression analyses. After infection with <i<S. agalactiae</i<, the content of reactive oxygen species (ROS) in serum was significantly increased (<i<p</i< < 0.05). Serum levels of glucose (GLU), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and malondialdehyde (MDA) increased and then decreased (<i<p</i< < 0.05), reaching a maximum at 6 h. Serum antioxidant enzyme (LZM) activity increased significantly (<i<p</i< < 0.05) and reached a maximum at 120 h. In addition, the mRNA expression levels of antioxidant genes (<i<SOD</i<, <i<CAT</i<, and <i<GPx</i<) in the liver increased and then decreased, reaching the maximum at 24 h, 48 h, and 24 h, respectively. During the experimental period, the mRNA expression levels of NF-κB-related genes of the inflammatory signaling pathway inhibitory κB (<i<IκB</i<) showed an overall decreasing trend (<i<p</i< < 0.05) and the lowest expression at 120 h, whereas the mRNA expression levels of tumor necrosis factor α (<i<TNF-α</i<), interleukin-1β (<i<IL-1β</i<), IκB kinase (<i<IKK</i<), and nuclear factor NF-κB increased significantly (<i<p</i< < 0.05) and the highest expression was at 120 h. In conclusion, these results showed that <i<S. agalactiae</i< could activate internal regulatory signaling in the liver of golden pompano to induce defense and immune responses. This study is expected to lay a foundation to develop the healthy aquaculture of golden pompano and promote a more comprehensive understanding of its disease resistance mechanisms.
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container_issue	9, p 1809
title_short	ROS Induced by <i<Streptococcus agalactiae</i< Activate Inflammatory Responses via the TNF-α/NF-κB Signaling Pathway in Golden Pompano <i<Trachinotus ovatus</i< (Linnaeus, 1758)
url	https://doi.org/10.3390/antiox11091809 https://doaj.org/article/9c41f15bf1124080a85157ee7aa175d8 https://www.mdpi.com/2076-3921/11/9/1809 https://doaj.org/toc/2076-3921
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ROS Induced by <i<Streptococcus agalactiae</i< Activate Inflammatory Responses via the TNF-α/NF-κB Signaling Pathway in Golden Pompano <i<Trachinotus ovatus</i< (Linnaeus, 1758)

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