Interlaboratory Validation of Toxicity Testing Using the Duckweed <i<Lemna minor</i< Root-Regrowth Test

The common duckweed (<i<Lemna minor</i<), a freshwater monocot that floats on the surfaces of slow-moving streams and ponds, is commonly used in toxicity testing. The novel <i<Lemna</i< root- regrowth test is a toxicity test performed in replicate test vessels (24-well plates...
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Autor*in:	Jihae Park [verfasserIn] Eun-Jin Yoo [verfasserIn] Kisik Shin [verfasserIn] Stephen Depuydt [verfasserIn] Wei Li [verfasserIn] Klaus-J. Appenroth [verfasserIn] Adam D. Lillicrap [verfasserIn] Li Xie [verfasserIn] Hojun Lee [verfasserIn] Geehyoung Kim [verfasserIn] Jonas De Saeger [verfasserIn] Soyeon Choi [verfasserIn] Geonhee Kim [verfasserIn] Murray T. Brown [verfasserIn] Taejun Han [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	interlaboratory comparison internationalized methods metals root regrowth

Übergeordnetes Werk:	In: Biology - MDPI AG, 2012, 11(2021), 1, p 37
Übergeordnetes Werk:	volume:11 ; year:2021 ; number:1, p 37

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/biology11010037

Katalog-ID:	DOAJ084860413

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author	Jihae Park
spellingShingle	Jihae Park misc QH301-705.5 misc interlaboratory comparison misc internationalized methods misc <i<Lemna minor</i< misc metals misc root regrowth misc Biology (General) Interlaboratory Validation of Toxicity Testing Using the Duckweed <i<Lemna minor</i< Root-Regrowth Test
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topic_title	QH301-705.5 Interlaboratory Validation of Toxicity Testing Using the Duckweed <i<Lemna minor</i< Root-Regrowth Test interlaboratory comparison internationalized methods <i<Lemna minor</i< metals root regrowth
topic	misc QH301-705.5 misc interlaboratory comparison misc internationalized methods misc <i<Lemna minor</i< misc metals misc root regrowth misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc interlaboratory comparison misc internationalized methods misc <i<Lemna minor</i< misc metals misc root regrowth misc Biology (General)
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title	Interlaboratory Validation of Toxicity Testing Using the Duckweed <i<Lemna minor</i< Root-Regrowth Test
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title_full	Interlaboratory Validation of Toxicity Testing Using the Duckweed <i<Lemna minor</i< Root-Regrowth Test
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title_sort	interlaboratory validation of toxicity testing using the duckweed <i<lemna minor</i< root-regrowth test
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title_auth	Interlaboratory Validation of Toxicity Testing Using the Duckweed <i<Lemna minor</i< Root-Regrowth Test
abstract	The common duckweed (<i<Lemna minor</i<), a freshwater monocot that floats on the surfaces of slow-moving streams and ponds, is commonly used in toxicity testing. The novel <i<Lemna</i< root- regrowth test is a toxicity test performed in replicate test vessels (24-well plates), each containing 3 mL test solution and a 2–3 frond colony. Prior to exposure, roots are excised from the plant, and newly developed roots are measured after 3 days of regrowth. Compared to the three internationally standardized methods, this bioassay is faster (72 h), simpler, more convenient (requiring only a 3-mL) and cheaper. The sensitivity of root regrowth to 3,5-dichlorophenol was statistically the same as using the conventional ISO test method. The results of interlaboratory comparison tests conducted by 10 international institutes showed 21.3% repeatability and 27.2% reproducibility for CuSO<sub<4</sub< and 21.28% repeatability and 18.6% reproducibility for wastewater. These validity criteria are well within the generally accepted levels of <30% to 40%, confirming that this test method is acceptable as a standardized biological test and can be used as a regulatory tool. The <i<Lemna</i< root regrowth test complements the lengthier conventional protocols and is suitable for rapid screening of wastewater and priority substances spikes in natural waters.
abstractGer	The common duckweed (<i<Lemna minor</i<), a freshwater monocot that floats on the surfaces of slow-moving streams and ponds, is commonly used in toxicity testing. The novel <i<Lemna</i< root- regrowth test is a toxicity test performed in replicate test vessels (24-well plates), each containing 3 mL test solution and a 2–3 frond colony. Prior to exposure, roots are excised from the plant, and newly developed roots are measured after 3 days of regrowth. Compared to the three internationally standardized methods, this bioassay is faster (72 h), simpler, more convenient (requiring only a 3-mL) and cheaper. The sensitivity of root regrowth to 3,5-dichlorophenol was statistically the same as using the conventional ISO test method. The results of interlaboratory comparison tests conducted by 10 international institutes showed 21.3% repeatability and 27.2% reproducibility for CuSO<sub<4</sub< and 21.28% repeatability and 18.6% reproducibility for wastewater. These validity criteria are well within the generally accepted levels of <30% to 40%, confirming that this test method is acceptable as a standardized biological test and can be used as a regulatory tool. The <i<Lemna</i< root regrowth test complements the lengthier conventional protocols and is suitable for rapid screening of wastewater and priority substances spikes in natural waters.
abstract_unstemmed	The common duckweed (<i<Lemna minor</i<), a freshwater monocot that floats on the surfaces of slow-moving streams and ponds, is commonly used in toxicity testing. The novel <i<Lemna</i< root- regrowth test is a toxicity test performed in replicate test vessels (24-well plates), each containing 3 mL test solution and a 2–3 frond colony. Prior to exposure, roots are excised from the plant, and newly developed roots are measured after 3 days of regrowth. Compared to the three internationally standardized methods, this bioassay is faster (72 h), simpler, more convenient (requiring only a 3-mL) and cheaper. The sensitivity of root regrowth to 3,5-dichlorophenol was statistically the same as using the conventional ISO test method. The results of interlaboratory comparison tests conducted by 10 international institutes showed 21.3% repeatability and 27.2% reproducibility for CuSO<sub<4</sub< and 21.28% repeatability and 18.6% reproducibility for wastewater. These validity criteria are well within the generally accepted levels of <30% to 40%, confirming that this test method is acceptable as a standardized biological test and can be used as a regulatory tool. The <i<Lemna</i< root regrowth test complements the lengthier conventional protocols and is suitable for rapid screening of wastewater and priority substances spikes in natural waters.
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title_short	Interlaboratory Validation of Toxicity Testing Using the Duckweed <i<Lemna minor</i< Root-Regrowth Test
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The novel <i<Lemna</i< root- regrowth test is a toxicity test performed in replicate test vessels (24-well plates), each containing 3 mL test solution and a 2–3 frond colony. Prior to exposure, roots are excised from the plant, and newly developed roots are measured after 3 days of regrowth. Compared to the three internationally standardized methods, this bioassay is faster (72 h), simpler, more convenient (requiring only a 3-mL) and cheaper. The sensitivity of root regrowth to 3,5-dichlorophenol was statistically the same as using the conventional ISO test method. The results of interlaboratory comparison tests conducted by 10 international institutes showed 21.3% repeatability and 27.2% reproducibility for CuSO<sub<4</sub< and 21.28% repeatability and 18.6% reproducibility for wastewater. These validity criteria are well within the generally accepted levels of <30% to 40%, confirming that this test method is acceptable as a standardized biological test and can be used as a regulatory tool. 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Interlaboratory Validation of Toxicity Testing Using the Duckweed <i<Lemna minor</i< Root-Regrowth Test

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