Genome-Wide Identification of Polyamine Oxidase (PAO) Family Genes: Roles of <i<CaPAO2</i< and <i<CaPAO4</i< in the Cold Tolerance of Pepper (<i<Capsicum annuum</i< L.)

Polyamine oxidases (PAOs), which are flavin adenine dinucleotide-dependent enzymes, catalyze polyamine (PA) catabolism, producing hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). Several PAO family members have been identified in plants, but their expression in pepper plants r...
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Autor*in:	Jianwei Zhang [verfasserIn] Le Liang [verfasserIn] Jiachang Xiao [verfasserIn] Yongdong Xie [verfasserIn] Li Zhu [verfasserIn] Xinru Xue [verfasserIn] Linyu Xu [verfasserIn] Peihan Zhou [verfasserIn] Jianzhao Ran [verfasserIn] Zhi Huang [verfasserIn] Guochao Sun [verfasserIn] Yunsong Lai [verfasserIn] Bo Sun [verfasserIn] Yi Tang [verfasserIn] Huanxiu Li [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	pepper PAO gene family cold stress overexpression prokaryotic expression

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 23(2022), 17, p 9999
Übergeordnetes Werk:	volume:23 ; year:2022 ; number:17, p 9999

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms23179999

Katalog-ID:	DOAJ084936118

Internformat


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245	1	0	\|a Genome-Wide Identification of Polyamine Oxidase (PAO) Family Genes: Roles of <i<CaPAO2</i< and <i<CaPAO4</i< in the Cold Tolerance of Pepper (<i<Capsicum annuum</i< L.)
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520			\|a Polyamine oxidases (PAOs), which are flavin adenine dinucleotide-dependent enzymes, catalyze polyamine (PA) catabolism, producing hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). Several PAO family members have been identified in plants, but their expression in pepper plants remains unclear. Here, six PAO genes were identified in the ‘Zunla-1’ pepper genome (named <i<CaPAO1</i<–<i<CaPAO6</i< according to their chromosomal positions). The PAO proteins were divided into four subfamilies according to phylogenetics: CaPAO1 belongs to subfamily I; CaPAO3 and CaPAO5 belong to subfamily III; and CaPAO2, CaPAO4, and CaPAO6 belong to subfamily IV (none belong to subfamily II). <i<CaPAO2</i<, <i<CaPAO4</i<, and <i<CaPAO6</i< were ubiquitously and highly expressed in all tissues, <i<CaPAO1</i< was mainly expressed in flowers, whereas <i<CaPAO3</i< and <i<CaPAO5</i< were expressed at very low levels in all tissues. RNA-seq analysis revealed that <i<CaPAO2</i< and <i<CaPAO4</i< were notably upregulated by cold stress. CaPAO2 and CaPAO4 were localized in the peroxisome, and spermine was the preferred substrate for PA catabolism. <i<CaPAO2</i< and <i<CaPAO4</i< overexpression in <i<Arabidopsis thaliana</i< significantly enhanced freezing-stress tolerance by increasing antioxidant enzyme activity and decreasing malondialdehyde, H<sub<2</sub<O<sub<2</sub<, and superoxide accumulation, accompanied by the upregulation of cold-responsive genes (<i<AtCOR15A</i<, <i<AtRD29A</i<, <i<AtCOR47</i<, and <i<AtKIN1</i<). Thus, we identified candidate PAO genes for breeding cold-stress-tolerant transgenic pepper cultivars.
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Indexfelder

author_variant	j z jz l l ll j x jx y x yx l z lz x x xx l x lx p z pz j r jr z h zh g s gs y l yl b s bs y t yt h l hl
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allfields	10.3390/ijms23179999 doi (DE-627)DOAJ084936118 (DE-599)DOAJ5b7296aa0f8f4b1991ac471d0c281f34 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Jianwei Zhang verfasserin aut Genome-Wide Identification of Polyamine Oxidase (PAO) Family Genes: Roles of <i<CaPAO2</i< and <i<CaPAO4</i< in the Cold Tolerance of Pepper (<i<Capsicum annuum</i< L.) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Polyamine oxidases (PAOs), which are flavin adenine dinucleotide-dependent enzymes, catalyze polyamine (PA) catabolism, producing hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). Several PAO family members have been identified in plants, but their expression in pepper plants remains unclear. Here, six PAO genes were identified in the ‘Zunla-1’ pepper genome (named <i<CaPAO1</i<–<i<CaPAO6</i< according to their chromosomal positions). The PAO proteins were divided into four subfamilies according to phylogenetics: CaPAO1 belongs to subfamily I; CaPAO3 and CaPAO5 belong to subfamily III; and CaPAO2, CaPAO4, and CaPAO6 belong to subfamily IV (none belong to subfamily II). <i<CaPAO2</i<, <i<CaPAO4</i<, and <i<CaPAO6</i< were ubiquitously and highly expressed in all tissues, <i<CaPAO1</i< was mainly expressed in flowers, whereas <i<CaPAO3</i< and <i<CaPAO5</i< were expressed at very low levels in all tissues. RNA-seq analysis revealed that <i<CaPAO2</i< and <i<CaPAO4</i< were notably upregulated by cold stress. CaPAO2 and CaPAO4 were localized in the peroxisome, and spermine was the preferred substrate for PA catabolism. <i<CaPAO2</i< and <i<CaPAO4</i< overexpression in <i<Arabidopsis thaliana</i< significantly enhanced freezing-stress tolerance by increasing antioxidant enzyme activity and decreasing malondialdehyde, H<sub<2</sub<O<sub<2</sub<, and superoxide accumulation, accompanied by the upregulation of cold-responsive genes (<i<AtCOR15A</i<, <i<AtRD29A</i<, <i<AtCOR47</i<, and <i<AtKIN1</i<). Thus, we identified candidate PAO genes for breeding cold-stress-tolerant transgenic pepper cultivars. pepper PAO gene family cold stress overexpression prokaryotic expression Biology (General) Chemistry Le Liang verfasserin aut Jiachang Xiao verfasserin aut Yongdong Xie verfasserin aut Li Zhu verfasserin aut Xinru Xue verfasserin aut Linyu Xu verfasserin aut Peihan Zhou verfasserin aut Jianzhao Ran verfasserin aut Zhi Huang verfasserin aut Guochao Sun verfasserin aut Yunsong Lai verfasserin aut Bo Sun verfasserin aut Yi Tang verfasserin aut Huanxiu Li verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 17, p 9999 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:17, p 9999 https://doi.org/10.3390/ijms23179999 kostenfrei https://doaj.org/article/5b7296aa0f8f4b1991ac471d0c281f34 kostenfrei https://www.mdpi.com/1422-0067/23/17/9999 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 17, p 9999
spelling	10.3390/ijms23179999 doi (DE-627)DOAJ084936118 (DE-599)DOAJ5b7296aa0f8f4b1991ac471d0c281f34 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Jianwei Zhang verfasserin aut Genome-Wide Identification of Polyamine Oxidase (PAO) Family Genes: Roles of <i<CaPAO2</i< and <i<CaPAO4</i< in the Cold Tolerance of Pepper (<i<Capsicum annuum</i< L.) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Polyamine oxidases (PAOs), which are flavin adenine dinucleotide-dependent enzymes, catalyze polyamine (PA) catabolism, producing hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). Several PAO family members have been identified in plants, but their expression in pepper plants remains unclear. Here, six PAO genes were identified in the ‘Zunla-1’ pepper genome (named <i<CaPAO1</i<–<i<CaPAO6</i< according to their chromosomal positions). The PAO proteins were divided into four subfamilies according to phylogenetics: CaPAO1 belongs to subfamily I; CaPAO3 and CaPAO5 belong to subfamily III; and CaPAO2, CaPAO4, and CaPAO6 belong to subfamily IV (none belong to subfamily II). <i<CaPAO2</i<, <i<CaPAO4</i<, and <i<CaPAO6</i< were ubiquitously and highly expressed in all tissues, <i<CaPAO1</i< was mainly expressed in flowers, whereas <i<CaPAO3</i< and <i<CaPAO5</i< were expressed at very low levels in all tissues. RNA-seq analysis revealed that <i<CaPAO2</i< and <i<CaPAO4</i< were notably upregulated by cold stress. CaPAO2 and CaPAO4 were localized in the peroxisome, and spermine was the preferred substrate for PA catabolism. <i<CaPAO2</i< and <i<CaPAO4</i< overexpression in <i<Arabidopsis thaliana</i< significantly enhanced freezing-stress tolerance by increasing antioxidant enzyme activity and decreasing malondialdehyde, H<sub<2</sub<O<sub<2</sub<, and superoxide accumulation, accompanied by the upregulation of cold-responsive genes (<i<AtCOR15A</i<, <i<AtRD29A</i<, <i<AtCOR47</i<, and <i<AtKIN1</i<). Thus, we identified candidate PAO genes for breeding cold-stress-tolerant transgenic pepper cultivars. pepper PAO gene family cold stress overexpression prokaryotic expression Biology (General) Chemistry Le Liang verfasserin aut Jiachang Xiao verfasserin aut Yongdong Xie verfasserin aut Li Zhu verfasserin aut Xinru Xue verfasserin aut Linyu Xu verfasserin aut Peihan Zhou verfasserin aut Jianzhao Ran verfasserin aut Zhi Huang verfasserin aut Guochao Sun verfasserin aut Yunsong Lai verfasserin aut Bo Sun verfasserin aut Yi Tang verfasserin aut Huanxiu Li verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 17, p 9999 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:17, p 9999 https://doi.org/10.3390/ijms23179999 kostenfrei https://doaj.org/article/5b7296aa0f8f4b1991ac471d0c281f34 kostenfrei https://www.mdpi.com/1422-0067/23/17/9999 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 17, p 9999
allfields_unstemmed	10.3390/ijms23179999 doi (DE-627)DOAJ084936118 (DE-599)DOAJ5b7296aa0f8f4b1991ac471d0c281f34 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Jianwei Zhang verfasserin aut Genome-Wide Identification of Polyamine Oxidase (PAO) Family Genes: Roles of <i<CaPAO2</i< and <i<CaPAO4</i< in the Cold Tolerance of Pepper (<i<Capsicum annuum</i< L.) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Polyamine oxidases (PAOs), which are flavin adenine dinucleotide-dependent enzymes, catalyze polyamine (PA) catabolism, producing hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). Several PAO family members have been identified in plants, but their expression in pepper plants remains unclear. Here, six PAO genes were identified in the ‘Zunla-1’ pepper genome (named <i<CaPAO1</i<–<i<CaPAO6</i< according to their chromosomal positions). The PAO proteins were divided into four subfamilies according to phylogenetics: CaPAO1 belongs to subfamily I; CaPAO3 and CaPAO5 belong to subfamily III; and CaPAO2, CaPAO4, and CaPAO6 belong to subfamily IV (none belong to subfamily II). <i<CaPAO2</i<, <i<CaPAO4</i<, and <i<CaPAO6</i< were ubiquitously and highly expressed in all tissues, <i<CaPAO1</i< was mainly expressed in flowers, whereas <i<CaPAO3</i< and <i<CaPAO5</i< were expressed at very low levels in all tissues. RNA-seq analysis revealed that <i<CaPAO2</i< and <i<CaPAO4</i< were notably upregulated by cold stress. CaPAO2 and CaPAO4 were localized in the peroxisome, and spermine was the preferred substrate for PA catabolism. <i<CaPAO2</i< and <i<CaPAO4</i< overexpression in <i<Arabidopsis thaliana</i< significantly enhanced freezing-stress tolerance by increasing antioxidant enzyme activity and decreasing malondialdehyde, H<sub<2</sub<O<sub<2</sub<, and superoxide accumulation, accompanied by the upregulation of cold-responsive genes (<i<AtCOR15A</i<, <i<AtRD29A</i<, <i<AtCOR47</i<, and <i<AtKIN1</i<). Thus, we identified candidate PAO genes for breeding cold-stress-tolerant transgenic pepper cultivars. pepper PAO gene family cold stress overexpression prokaryotic expression Biology (General) Chemistry Le Liang verfasserin aut Jiachang Xiao verfasserin aut Yongdong Xie verfasserin aut Li Zhu verfasserin aut Xinru Xue verfasserin aut Linyu Xu verfasserin aut Peihan Zhou verfasserin aut Jianzhao Ran verfasserin aut Zhi Huang verfasserin aut Guochao Sun verfasserin aut Yunsong Lai verfasserin aut Bo Sun verfasserin aut Yi Tang verfasserin aut Huanxiu Li verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 17, p 9999 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:17, p 9999 https://doi.org/10.3390/ijms23179999 kostenfrei https://doaj.org/article/5b7296aa0f8f4b1991ac471d0c281f34 kostenfrei https://www.mdpi.com/1422-0067/23/17/9999 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 17, p 9999
allfieldsGer	10.3390/ijms23179999 doi (DE-627)DOAJ084936118 (DE-599)DOAJ5b7296aa0f8f4b1991ac471d0c281f34 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Jianwei Zhang verfasserin aut Genome-Wide Identification of Polyamine Oxidase (PAO) Family Genes: Roles of <i<CaPAO2</i< and <i<CaPAO4</i< in the Cold Tolerance of Pepper (<i<Capsicum annuum</i< L.) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Polyamine oxidases (PAOs), which are flavin adenine dinucleotide-dependent enzymes, catalyze polyamine (PA) catabolism, producing hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). Several PAO family members have been identified in plants, but their expression in pepper plants remains unclear. Here, six PAO genes were identified in the ‘Zunla-1’ pepper genome (named <i<CaPAO1</i<–<i<CaPAO6</i< according to their chromosomal positions). The PAO proteins were divided into four subfamilies according to phylogenetics: CaPAO1 belongs to subfamily I; CaPAO3 and CaPAO5 belong to subfamily III; and CaPAO2, CaPAO4, and CaPAO6 belong to subfamily IV (none belong to subfamily II). <i<CaPAO2</i<, <i<CaPAO4</i<, and <i<CaPAO6</i< were ubiquitously and highly expressed in all tissues, <i<CaPAO1</i< was mainly expressed in flowers, whereas <i<CaPAO3</i< and <i<CaPAO5</i< were expressed at very low levels in all tissues. RNA-seq analysis revealed that <i<CaPAO2</i< and <i<CaPAO4</i< were notably upregulated by cold stress. CaPAO2 and CaPAO4 were localized in the peroxisome, and spermine was the preferred substrate for PA catabolism. <i<CaPAO2</i< and <i<CaPAO4</i< overexpression in <i<Arabidopsis thaliana</i< significantly enhanced freezing-stress tolerance by increasing antioxidant enzyme activity and decreasing malondialdehyde, H<sub<2</sub<O<sub<2</sub<, and superoxide accumulation, accompanied by the upregulation of cold-responsive genes (<i<AtCOR15A</i<, <i<AtRD29A</i<, <i<AtCOR47</i<, and <i<AtKIN1</i<). Thus, we identified candidate PAO genes for breeding cold-stress-tolerant transgenic pepper cultivars. pepper PAO gene family cold stress overexpression prokaryotic expression Biology (General) Chemistry Le Liang verfasserin aut Jiachang Xiao verfasserin aut Yongdong Xie verfasserin aut Li Zhu verfasserin aut Xinru Xue verfasserin aut Linyu Xu verfasserin aut Peihan Zhou verfasserin aut Jianzhao Ran verfasserin aut Zhi Huang verfasserin aut Guochao Sun verfasserin aut Yunsong Lai verfasserin aut Bo Sun verfasserin aut Yi Tang verfasserin aut Huanxiu Li verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 17, p 9999 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:17, p 9999 https://doi.org/10.3390/ijms23179999 kostenfrei https://doaj.org/article/5b7296aa0f8f4b1991ac471d0c281f34 kostenfrei https://www.mdpi.com/1422-0067/23/17/9999 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 17, p 9999
allfieldsSound	10.3390/ijms23179999 doi (DE-627)DOAJ084936118 (DE-599)DOAJ5b7296aa0f8f4b1991ac471d0c281f34 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Jianwei Zhang verfasserin aut Genome-Wide Identification of Polyamine Oxidase (PAO) Family Genes: Roles of <i<CaPAO2</i< and <i<CaPAO4</i< in the Cold Tolerance of Pepper (<i<Capsicum annuum</i< L.) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Polyamine oxidases (PAOs), which are flavin adenine dinucleotide-dependent enzymes, catalyze polyamine (PA) catabolism, producing hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). Several PAO family members have been identified in plants, but their expression in pepper plants remains unclear. Here, six PAO genes were identified in the ‘Zunla-1’ pepper genome (named <i<CaPAO1</i<–<i<CaPAO6</i< according to their chromosomal positions). The PAO proteins were divided into four subfamilies according to phylogenetics: CaPAO1 belongs to subfamily I; CaPAO3 and CaPAO5 belong to subfamily III; and CaPAO2, CaPAO4, and CaPAO6 belong to subfamily IV (none belong to subfamily II). <i<CaPAO2</i<, <i<CaPAO4</i<, and <i<CaPAO6</i< were ubiquitously and highly expressed in all tissues, <i<CaPAO1</i< was mainly expressed in flowers, whereas <i<CaPAO3</i< and <i<CaPAO5</i< were expressed at very low levels in all tissues. RNA-seq analysis revealed that <i<CaPAO2</i< and <i<CaPAO4</i< were notably upregulated by cold stress. CaPAO2 and CaPAO4 were localized in the peroxisome, and spermine was the preferred substrate for PA catabolism. <i<CaPAO2</i< and <i<CaPAO4</i< overexpression in <i<Arabidopsis thaliana</i< significantly enhanced freezing-stress tolerance by increasing antioxidant enzyme activity and decreasing malondialdehyde, H<sub<2</sub<O<sub<2</sub<, and superoxide accumulation, accompanied by the upregulation of cold-responsive genes (<i<AtCOR15A</i<, <i<AtRD29A</i<, <i<AtCOR47</i<, and <i<AtKIN1</i<). Thus, we identified candidate PAO genes for breeding cold-stress-tolerant transgenic pepper cultivars. pepper PAO gene family cold stress overexpression prokaryotic expression Biology (General) Chemistry Le Liang verfasserin aut Jiachang Xiao verfasserin aut Yongdong Xie verfasserin aut Li Zhu verfasserin aut Xinru Xue verfasserin aut Linyu Xu verfasserin aut Peihan Zhou verfasserin aut Jianzhao Ran verfasserin aut Zhi Huang verfasserin aut Guochao Sun verfasserin aut Yunsong Lai verfasserin aut Bo Sun verfasserin aut Yi Tang verfasserin aut Huanxiu Li verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 17, p 9999 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:17, p 9999 https://doi.org/10.3390/ijms23179999 kostenfrei https://doaj.org/article/5b7296aa0f8f4b1991ac471d0c281f34 kostenfrei https://www.mdpi.com/1422-0067/23/17/9999 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 17, p 9999
language	English
source	In International Journal of Molecular Sciences 23(2022), 17, p 9999 volume:23 year:2022 number:17, p 9999
sourceStr	In International Journal of Molecular Sciences 23(2022), 17, p 9999 volume:23 year:2022 number:17, p 9999
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	pepper PAO gene family cold stress overexpression prokaryotic expression Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Jianwei Zhang @@aut@@ Le Liang @@aut@@ Jiachang Xiao @@aut@@ Yongdong Xie @@aut@@ Li Zhu @@aut@@ Xinru Xue @@aut@@ Linyu Xu @@aut@@ Peihan Zhou @@aut@@ Jianzhao Ran @@aut@@ Zhi Huang @@aut@@ Guochao Sun @@aut@@ Yunsong Lai @@aut@@ Bo Sun @@aut@@ Yi Tang @@aut@@ Huanxiu Li @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ084936118
language_de	englisch
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Several PAO family members have been identified in plants, but their expression in pepper plants remains unclear. Here, six PAO genes were identified in the ‘Zunla-1’ pepper genome (named <i<CaPAO1</i<–<i<CaPAO6</i< according to their chromosomal positions). The PAO proteins were divided into four subfamilies according to phylogenetics: CaPAO1 belongs to subfamily I; CaPAO3 and CaPAO5 belong to subfamily III; and CaPAO2, CaPAO4, and CaPAO6 belong to subfamily IV (none belong to subfamily II). <i<CaPAO2</i<, <i<CaPAO4</i<, and <i<CaPAO6</i< were ubiquitously and highly expressed in all tissues, <i<CaPAO1</i< was mainly expressed in flowers, whereas <i<CaPAO3</i< and <i<CaPAO5</i< were expressed at very low levels in all tissues. RNA-seq analysis revealed that <i<CaPAO2</i< and <i<CaPAO4</i< were notably upregulated by cold stress. CaPAO2 and CaPAO4 were localized in the peroxisome, and spermine was the preferred substrate for PA catabolism. <i<CaPAO2</i< and <i<CaPAO4</i< overexpression in <i<Arabidopsis thaliana</i< significantly enhanced freezing-stress tolerance by increasing antioxidant enzyme activity and decreasing malondialdehyde, H<sub<2</sub<O<sub<2</sub<, and superoxide accumulation, accompanied by the upregulation of cold-responsive genes (<i<AtCOR15A</i<, <i<AtRD29A</i<, <i<AtCOR47</i<, and <i<AtKIN1</i<). 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callnumber-first	Q - Science
author	Jianwei Zhang
spellingShingle	Jianwei Zhang misc QH301-705.5 misc QD1-999 misc pepper misc PAO gene family misc cold stress misc overexpression misc prokaryotic expression misc Biology (General) misc Chemistry Genome-Wide Identification of Polyamine Oxidase (PAO) Family Genes: Roles of <i<CaPAO2</i< and <i<CaPAO4</i< in the Cold Tolerance of Pepper (<i<Capsicum annuum</i< L.)
authorStr	Jianwei Zhang
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topic_title	QH301-705.5 QD1-999 Genome-Wide Identification of Polyamine Oxidase (PAO) Family Genes: Roles of <i<CaPAO2</i< and <i<CaPAO4</i< in the Cold Tolerance of Pepper (<i<Capsicum annuum</i< L.) pepper PAO gene family cold stress overexpression prokaryotic expression
topic	misc QH301-705.5 misc QD1-999 misc pepper misc PAO gene family misc cold stress misc overexpression misc prokaryotic expression misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc pepper misc PAO gene family misc cold stress misc overexpression misc prokaryotic expression misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc pepper misc PAO gene family misc cold stress misc overexpression misc prokaryotic expression misc Biology (General) misc Chemistry
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title	Genome-Wide Identification of Polyamine Oxidase (PAO) Family Genes: Roles of <i<CaPAO2</i< and <i<CaPAO4</i< in the Cold Tolerance of Pepper (<i<Capsicum annuum</i< L.)
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title_full	Genome-Wide Identification of Polyamine Oxidase (PAO) Family Genes: Roles of <i<CaPAO2</i< and <i<CaPAO4</i< in the Cold Tolerance of Pepper (<i<Capsicum annuum</i< L.)
author_sort	Jianwei Zhang
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author_browse	Jianwei Zhang Le Liang Jiachang Xiao Yongdong Xie Li Zhu Xinru Xue Linyu Xu Peihan Zhou Jianzhao Ran Zhi Huang Guochao Sun Yunsong Lai Bo Sun Yi Tang Huanxiu Li
container_volume	23
class	QH301-705.5 QD1-999
format_se	Elektronische Aufsätze
author-letter	Jianwei Zhang
doi_str_mv	10.3390/ijms23179999
author2-role	verfasserin
title_sort	genome-wide identification of polyamine oxidase (pao) family genes: roles of <i<capao2</i< and <i<capao4</i< in the cold tolerance of pepper (<i<capsicum annuum</i< l.)
callnumber	QH301-705.5
title_auth	Genome-Wide Identification of Polyamine Oxidase (PAO) Family Genes: Roles of <i<CaPAO2</i< and <i<CaPAO4</i< in the Cold Tolerance of Pepper (<i<Capsicum annuum</i< L.)
abstract	Polyamine oxidases (PAOs), which are flavin adenine dinucleotide-dependent enzymes, catalyze polyamine (PA) catabolism, producing hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). Several PAO family members have been identified in plants, but their expression in pepper plants remains unclear. Here, six PAO genes were identified in the ‘Zunla-1’ pepper genome (named <i<CaPAO1</i<–<i<CaPAO6</i< according to their chromosomal positions). The PAO proteins were divided into four subfamilies according to phylogenetics: CaPAO1 belongs to subfamily I; CaPAO3 and CaPAO5 belong to subfamily III; and CaPAO2, CaPAO4, and CaPAO6 belong to subfamily IV (none belong to subfamily II). <i<CaPAO2</i<, <i<CaPAO4</i<, and <i<CaPAO6</i< were ubiquitously and highly expressed in all tissues, <i<CaPAO1</i< was mainly expressed in flowers, whereas <i<CaPAO3</i< and <i<CaPAO5</i< were expressed at very low levels in all tissues. RNA-seq analysis revealed that <i<CaPAO2</i< and <i<CaPAO4</i< were notably upregulated by cold stress. CaPAO2 and CaPAO4 were localized in the peroxisome, and spermine was the preferred substrate for PA catabolism. <i<CaPAO2</i< and <i<CaPAO4</i< overexpression in <i<Arabidopsis thaliana</i< significantly enhanced freezing-stress tolerance by increasing antioxidant enzyme activity and decreasing malondialdehyde, H<sub<2</sub<O<sub<2</sub<, and superoxide accumulation, accompanied by the upregulation of cold-responsive genes (<i<AtCOR15A</i<, <i<AtRD29A</i<, <i<AtCOR47</i<, and <i<AtKIN1</i<). Thus, we identified candidate PAO genes for breeding cold-stress-tolerant transgenic pepper cultivars.
abstractGer	Polyamine oxidases (PAOs), which are flavin adenine dinucleotide-dependent enzymes, catalyze polyamine (PA) catabolism, producing hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). Several PAO family members have been identified in plants, but their expression in pepper plants remains unclear. Here, six PAO genes were identified in the ‘Zunla-1’ pepper genome (named <i<CaPAO1</i<–<i<CaPAO6</i< according to their chromosomal positions). The PAO proteins were divided into four subfamilies according to phylogenetics: CaPAO1 belongs to subfamily I; CaPAO3 and CaPAO5 belong to subfamily III; and CaPAO2, CaPAO4, and CaPAO6 belong to subfamily IV (none belong to subfamily II). <i<CaPAO2</i<, <i<CaPAO4</i<, and <i<CaPAO6</i< were ubiquitously and highly expressed in all tissues, <i<CaPAO1</i< was mainly expressed in flowers, whereas <i<CaPAO3</i< and <i<CaPAO5</i< were expressed at very low levels in all tissues. RNA-seq analysis revealed that <i<CaPAO2</i< and <i<CaPAO4</i< were notably upregulated by cold stress. CaPAO2 and CaPAO4 were localized in the peroxisome, and spermine was the preferred substrate for PA catabolism. <i<CaPAO2</i< and <i<CaPAO4</i< overexpression in <i<Arabidopsis thaliana</i< significantly enhanced freezing-stress tolerance by increasing antioxidant enzyme activity and decreasing malondialdehyde, H<sub<2</sub<O<sub<2</sub<, and superoxide accumulation, accompanied by the upregulation of cold-responsive genes (<i<AtCOR15A</i<, <i<AtRD29A</i<, <i<AtCOR47</i<, and <i<AtKIN1</i<). Thus, we identified candidate PAO genes for breeding cold-stress-tolerant transgenic pepper cultivars.
abstract_unstemmed	Polyamine oxidases (PAOs), which are flavin adenine dinucleotide-dependent enzymes, catalyze polyamine (PA) catabolism, producing hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). Several PAO family members have been identified in plants, but their expression in pepper plants remains unclear. Here, six PAO genes were identified in the ‘Zunla-1’ pepper genome (named <i<CaPAO1</i<–<i<CaPAO6</i< according to their chromosomal positions). The PAO proteins were divided into four subfamilies according to phylogenetics: CaPAO1 belongs to subfamily I; CaPAO3 and CaPAO5 belong to subfamily III; and CaPAO2, CaPAO4, and CaPAO6 belong to subfamily IV (none belong to subfamily II). <i<CaPAO2</i<, <i<CaPAO4</i<, and <i<CaPAO6</i< were ubiquitously and highly expressed in all tissues, <i<CaPAO1</i< was mainly expressed in flowers, whereas <i<CaPAO3</i< and <i<CaPAO5</i< were expressed at very low levels in all tissues. RNA-seq analysis revealed that <i<CaPAO2</i< and <i<CaPAO4</i< were notably upregulated by cold stress. CaPAO2 and CaPAO4 were localized in the peroxisome, and spermine was the preferred substrate for PA catabolism. <i<CaPAO2</i< and <i<CaPAO4</i< overexpression in <i<Arabidopsis thaliana</i< significantly enhanced freezing-stress tolerance by increasing antioxidant enzyme activity and decreasing malondialdehyde, H<sub<2</sub<O<sub<2</sub<, and superoxide accumulation, accompanied by the upregulation of cold-responsive genes (<i<AtCOR15A</i<, <i<AtRD29A</i<, <i<AtCOR47</i<, and <i<AtKIN1</i<). Thus, we identified candidate PAO genes for breeding cold-stress-tolerant transgenic pepper cultivars.
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container_issue	17, p 9999
title_short	Genome-Wide Identification of Polyamine Oxidase (PAO) Family Genes: Roles of <i<CaPAO2</i< and <i<CaPAO4</i< in the Cold Tolerance of Pepper (<i<Capsicum annuum</i< L.)
url	https://doi.org/10.3390/ijms23179999 https://doaj.org/article/5b7296aa0f8f4b1991ac471d0c281f34 https://www.mdpi.com/1422-0067/23/17/9999 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
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Several PAO family members have been identified in plants, but their expression in pepper plants remains unclear. Here, six PAO genes were identified in the ‘Zunla-1’ pepper genome (named <i<CaPAO1</i<–<i<CaPAO6</i< according to their chromosomal positions). The PAO proteins were divided into four subfamilies according to phylogenetics: CaPAO1 belongs to subfamily I; CaPAO3 and CaPAO5 belong to subfamily III; and CaPAO2, CaPAO4, and CaPAO6 belong to subfamily IV (none belong to subfamily II). <i<CaPAO2</i<, <i<CaPAO4</i<, and <i<CaPAO6</i< were ubiquitously and highly expressed in all tissues, <i<CaPAO1</i< was mainly expressed in flowers, whereas <i<CaPAO3</i< and <i<CaPAO5</i< were expressed at very low levels in all tissues. RNA-seq analysis revealed that <i<CaPAO2</i< and <i<CaPAO4</i< were notably upregulated by cold stress. CaPAO2 and CaPAO4 were localized in the peroxisome, and spermine was the preferred substrate for PA catabolism. <i<CaPAO2</i< and <i<CaPAO4</i< overexpression in <i<Arabidopsis thaliana</i< significantly enhanced freezing-stress tolerance by increasing antioxidant enzyme activity and decreasing malondialdehyde, H<sub<2</sub<O<sub<2</sub<, and superoxide accumulation, accompanied by the upregulation of cold-responsive genes (<i<AtCOR15A</i<, <i<AtRD29A</i<, <i<AtCOR47</i<, and <i<AtKIN1</i<). 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Genome-Wide Identification of Polyamine Oxidase (PAO) Family Genes: Roles of <i<CaPAO2</i< and <i<CaPAO4</i< in the Cold Tolerance of Pepper (<i<Capsicum annuum</i< L.)

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