Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing

Powdery mildew of wheat (Triticum aestivum), caused by Blumeria graminis f.sp. tritici (Bgt), is a destructive disease that seriously threatens the yield and quality of its host. Identifying resistance genes is the most attractive and effective strategy for developing disease-resistant cultivars and...
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Autor*in:	Yanjun Mu [verfasserIn] Wenping Gong [verfasserIn] Yanmin Qie [verfasserIn] Xueqing Liu [verfasserIn] Linzhi Li [verfasserIn] Nina Sun [verfasserIn] Wei Liu [verfasserIn] Jun Guo [verfasserIn] Ran Han [verfasserIn] Ziyang Yu [verfasserIn] Luning Xiao [verfasserIn] Fuyu Su [verfasserIn] Wenjing Zhang [verfasserIn] Jiangchun Wang [verfasserIn] Guohao Han [verfasserIn] Pengtao Ma [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	wheat powdery mildew pmYN99102 MAS BSE-Seq molecular mapping

Übergeordnetes Werk:	In: Frontiers in Plant Science - Frontiers Media S.A., 2011, 13(2022)
Übergeordnetes Werk:	volume:13 ; year:2022

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fpls.2022.1005627

Katalog-ID:	DOAJ084960442

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allfields	10.3389/fpls.2022.1005627 doi (DE-627)DOAJ084960442 (DE-599)DOAJb38fe46f282d4119b08337b8d1841e96 DE-627 ger DE-627 rakwb eng SB1-1110 Yanjun Mu verfasserin aut Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Powdery mildew of wheat (Triticum aestivum), caused by Blumeria graminis f.sp. tritici (Bgt), is a destructive disease that seriously threatens the yield and quality of its host. Identifying resistance genes is the most attractive and effective strategy for developing disease-resistant cultivars and controlling this disease. In this study, a wheat breeding line Yannong 99102-06188 (YN99102), an elite derivative line from the same breeding process as the famous wheat cultivar Yannong 999, showed high resistance to powdery mildew at the whole growth stages. Genetic analysis was carried out using Bgt isolate E09 and a population of YN99102 crossed with a susceptible parent Jinhe 13–205 (JH13–205). The result indicated that a single recessive gene, tentatively designated pmYN99102, conferred seedling resistance to the Bgt isolate E09. Using bulked segregant exome capture sequencing (BSE-Seq), pmYN99102 was physically located to a ~33.7 Mb (691.0–724.7 Mb) interval on the chromosome arm 2BL, and this interval was further locked in a 1.5 cM genetic interval using molecular markers, which was aligned to a 9.0 Mb physical interval (699.2–708.2 Mb). Based on the analysis of physical location, origin, resistant spectrum, and inherited pattern, pmYN99102 differed from those of the reported powdery mildew (Pm) resistance genes on 2BL, suggesting pmYN99102 is most likely a new Pm gene/allele in the targeted interval. To transfer pmYN99102 to different genetic backgrounds using marker-assisted selection (MAS), 18 closely linked markers were tested for their availability in different genetic backgrounds for MAS, and all markers expect for YTU103-97 can be used in MAS for tracking pmYN99102 when it transferred into those susceptible cultivars. wheat powdery mildew pmYN99102 MAS BSE-Seq molecular mapping Plant culture Wenping Gong verfasserin aut Yanmin Qie verfasserin aut Xueqing Liu verfasserin aut Linzhi Li verfasserin aut Nina Sun verfasserin aut Wei Liu verfasserin aut Jun Guo verfasserin aut Ran Han verfasserin aut Ziyang Yu verfasserin aut Luning Xiao verfasserin aut Fuyu Su verfasserin aut Wenjing Zhang verfasserin aut Jiangchun Wang verfasserin aut Guohao Han verfasserin aut Pengtao Ma verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 13(2022) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:13 year:2022 https://doi.org/10.3389/fpls.2022.1005627 kostenfrei https://doaj.org/article/b38fe46f282d4119b08337b8d1841e96 kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2022.1005627/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022
spelling	10.3389/fpls.2022.1005627 doi (DE-627)DOAJ084960442 (DE-599)DOAJb38fe46f282d4119b08337b8d1841e96 DE-627 ger DE-627 rakwb eng SB1-1110 Yanjun Mu verfasserin aut Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Powdery mildew of wheat (Triticum aestivum), caused by Blumeria graminis f.sp. tritici (Bgt), is a destructive disease that seriously threatens the yield and quality of its host. Identifying resistance genes is the most attractive and effective strategy for developing disease-resistant cultivars and controlling this disease. In this study, a wheat breeding line Yannong 99102-06188 (YN99102), an elite derivative line from the same breeding process as the famous wheat cultivar Yannong 999, showed high resistance to powdery mildew at the whole growth stages. Genetic analysis was carried out using Bgt isolate E09 and a population of YN99102 crossed with a susceptible parent Jinhe 13–205 (JH13–205). The result indicated that a single recessive gene, tentatively designated pmYN99102, conferred seedling resistance to the Bgt isolate E09. Using bulked segregant exome capture sequencing (BSE-Seq), pmYN99102 was physically located to a ~33.7 Mb (691.0–724.7 Mb) interval on the chromosome arm 2BL, and this interval was further locked in a 1.5 cM genetic interval using molecular markers, which was aligned to a 9.0 Mb physical interval (699.2–708.2 Mb). Based on the analysis of physical location, origin, resistant spectrum, and inherited pattern, pmYN99102 differed from those of the reported powdery mildew (Pm) resistance genes on 2BL, suggesting pmYN99102 is most likely a new Pm gene/allele in the targeted interval. To transfer pmYN99102 to different genetic backgrounds using marker-assisted selection (MAS), 18 closely linked markers were tested for their availability in different genetic backgrounds for MAS, and all markers expect for YTU103-97 can be used in MAS for tracking pmYN99102 when it transferred into those susceptible cultivars. wheat powdery mildew pmYN99102 MAS BSE-Seq molecular mapping Plant culture Wenping Gong verfasserin aut Yanmin Qie verfasserin aut Xueqing Liu verfasserin aut Linzhi Li verfasserin aut Nina Sun verfasserin aut Wei Liu verfasserin aut Jun Guo verfasserin aut Ran Han verfasserin aut Ziyang Yu verfasserin aut Luning Xiao verfasserin aut Fuyu Su verfasserin aut Wenjing Zhang verfasserin aut Jiangchun Wang verfasserin aut Guohao Han verfasserin aut Pengtao Ma verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 13(2022) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:13 year:2022 https://doi.org/10.3389/fpls.2022.1005627 kostenfrei https://doaj.org/article/b38fe46f282d4119b08337b8d1841e96 kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2022.1005627/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022
allfields_unstemmed	10.3389/fpls.2022.1005627 doi (DE-627)DOAJ084960442 (DE-599)DOAJb38fe46f282d4119b08337b8d1841e96 DE-627 ger DE-627 rakwb eng SB1-1110 Yanjun Mu verfasserin aut Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Powdery mildew of wheat (Triticum aestivum), caused by Blumeria graminis f.sp. tritici (Bgt), is a destructive disease that seriously threatens the yield and quality of its host. Identifying resistance genes is the most attractive and effective strategy for developing disease-resistant cultivars and controlling this disease. In this study, a wheat breeding line Yannong 99102-06188 (YN99102), an elite derivative line from the same breeding process as the famous wheat cultivar Yannong 999, showed high resistance to powdery mildew at the whole growth stages. Genetic analysis was carried out using Bgt isolate E09 and a population of YN99102 crossed with a susceptible parent Jinhe 13–205 (JH13–205). The result indicated that a single recessive gene, tentatively designated pmYN99102, conferred seedling resistance to the Bgt isolate E09. Using bulked segregant exome capture sequencing (BSE-Seq), pmYN99102 was physically located to a ~33.7 Mb (691.0–724.7 Mb) interval on the chromosome arm 2BL, and this interval was further locked in a 1.5 cM genetic interval using molecular markers, which was aligned to a 9.0 Mb physical interval (699.2–708.2 Mb). Based on the analysis of physical location, origin, resistant spectrum, and inherited pattern, pmYN99102 differed from those of the reported powdery mildew (Pm) resistance genes on 2BL, suggesting pmYN99102 is most likely a new Pm gene/allele in the targeted interval. To transfer pmYN99102 to different genetic backgrounds using marker-assisted selection (MAS), 18 closely linked markers were tested for their availability in different genetic backgrounds for MAS, and all markers expect for YTU103-97 can be used in MAS for tracking pmYN99102 when it transferred into those susceptible cultivars. wheat powdery mildew pmYN99102 MAS BSE-Seq molecular mapping Plant culture Wenping Gong verfasserin aut Yanmin Qie verfasserin aut Xueqing Liu verfasserin aut Linzhi Li verfasserin aut Nina Sun verfasserin aut Wei Liu verfasserin aut Jun Guo verfasserin aut Ran Han verfasserin aut Ziyang Yu verfasserin aut Luning Xiao verfasserin aut Fuyu Su verfasserin aut Wenjing Zhang verfasserin aut Jiangchun Wang verfasserin aut Guohao Han verfasserin aut Pengtao Ma verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 13(2022) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:13 year:2022 https://doi.org/10.3389/fpls.2022.1005627 kostenfrei https://doaj.org/article/b38fe46f282d4119b08337b8d1841e96 kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2022.1005627/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022
allfieldsGer	10.3389/fpls.2022.1005627 doi (DE-627)DOAJ084960442 (DE-599)DOAJb38fe46f282d4119b08337b8d1841e96 DE-627 ger DE-627 rakwb eng SB1-1110 Yanjun Mu verfasserin aut Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Powdery mildew of wheat (Triticum aestivum), caused by Blumeria graminis f.sp. tritici (Bgt), is a destructive disease that seriously threatens the yield and quality of its host. Identifying resistance genes is the most attractive and effective strategy for developing disease-resistant cultivars and controlling this disease. In this study, a wheat breeding line Yannong 99102-06188 (YN99102), an elite derivative line from the same breeding process as the famous wheat cultivar Yannong 999, showed high resistance to powdery mildew at the whole growth stages. Genetic analysis was carried out using Bgt isolate E09 and a population of YN99102 crossed with a susceptible parent Jinhe 13–205 (JH13–205). The result indicated that a single recessive gene, tentatively designated pmYN99102, conferred seedling resistance to the Bgt isolate E09. Using bulked segregant exome capture sequencing (BSE-Seq), pmYN99102 was physically located to a ~33.7 Mb (691.0–724.7 Mb) interval on the chromosome arm 2BL, and this interval was further locked in a 1.5 cM genetic interval using molecular markers, which was aligned to a 9.0 Mb physical interval (699.2–708.2 Mb). Based on the analysis of physical location, origin, resistant spectrum, and inherited pattern, pmYN99102 differed from those of the reported powdery mildew (Pm) resistance genes on 2BL, suggesting pmYN99102 is most likely a new Pm gene/allele in the targeted interval. To transfer pmYN99102 to different genetic backgrounds using marker-assisted selection (MAS), 18 closely linked markers were tested for their availability in different genetic backgrounds for MAS, and all markers expect for YTU103-97 can be used in MAS for tracking pmYN99102 when it transferred into those susceptible cultivars. wheat powdery mildew pmYN99102 MAS BSE-Seq molecular mapping Plant culture Wenping Gong verfasserin aut Yanmin Qie verfasserin aut Xueqing Liu verfasserin aut Linzhi Li verfasserin aut Nina Sun verfasserin aut Wei Liu verfasserin aut Jun Guo verfasserin aut Ran Han verfasserin aut Ziyang Yu verfasserin aut Luning Xiao verfasserin aut Fuyu Su verfasserin aut Wenjing Zhang verfasserin aut Jiangchun Wang verfasserin aut Guohao Han verfasserin aut Pengtao Ma verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 13(2022) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:13 year:2022 https://doi.org/10.3389/fpls.2022.1005627 kostenfrei https://doaj.org/article/b38fe46f282d4119b08337b8d1841e96 kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2022.1005627/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022
allfieldsSound	10.3389/fpls.2022.1005627 doi (DE-627)DOAJ084960442 (DE-599)DOAJb38fe46f282d4119b08337b8d1841e96 DE-627 ger DE-627 rakwb eng SB1-1110 Yanjun Mu verfasserin aut Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Powdery mildew of wheat (Triticum aestivum), caused by Blumeria graminis f.sp. tritici (Bgt), is a destructive disease that seriously threatens the yield and quality of its host. Identifying resistance genes is the most attractive and effective strategy for developing disease-resistant cultivars and controlling this disease. In this study, a wheat breeding line Yannong 99102-06188 (YN99102), an elite derivative line from the same breeding process as the famous wheat cultivar Yannong 999, showed high resistance to powdery mildew at the whole growth stages. Genetic analysis was carried out using Bgt isolate E09 and a population of YN99102 crossed with a susceptible parent Jinhe 13–205 (JH13–205). The result indicated that a single recessive gene, tentatively designated pmYN99102, conferred seedling resistance to the Bgt isolate E09. Using bulked segregant exome capture sequencing (BSE-Seq), pmYN99102 was physically located to a ~33.7 Mb (691.0–724.7 Mb) interval on the chromosome arm 2BL, and this interval was further locked in a 1.5 cM genetic interval using molecular markers, which was aligned to a 9.0 Mb physical interval (699.2–708.2 Mb). Based on the analysis of physical location, origin, resistant spectrum, and inherited pattern, pmYN99102 differed from those of the reported powdery mildew (Pm) resistance genes on 2BL, suggesting pmYN99102 is most likely a new Pm gene/allele in the targeted interval. To transfer pmYN99102 to different genetic backgrounds using marker-assisted selection (MAS), 18 closely linked markers were tested for their availability in different genetic backgrounds for MAS, and all markers expect for YTU103-97 can be used in MAS for tracking pmYN99102 when it transferred into those susceptible cultivars. wheat powdery mildew pmYN99102 MAS BSE-Seq molecular mapping Plant culture Wenping Gong verfasserin aut Yanmin Qie verfasserin aut Xueqing Liu verfasserin aut Linzhi Li verfasserin aut Nina Sun verfasserin aut Wei Liu verfasserin aut Jun Guo verfasserin aut Ran Han verfasserin aut Ziyang Yu verfasserin aut Luning Xiao verfasserin aut Fuyu Su verfasserin aut Wenjing Zhang verfasserin aut Jiangchun Wang verfasserin aut Guohao Han verfasserin aut Pengtao Ma verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 13(2022) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:13 year:2022 https://doi.org/10.3389/fpls.2022.1005627 kostenfrei https://doaj.org/article/b38fe46f282d4119b08337b8d1841e96 kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2022.1005627/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022
language	English
source	In Frontiers in Plant Science 13(2022) volume:13 year:2022
sourceStr	In Frontiers in Plant Science 13(2022) volume:13 year:2022
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	wheat powdery mildew pmYN99102 MAS BSE-Seq molecular mapping Plant culture
isfreeaccess_bool	true
container_title	Frontiers in Plant Science
authorswithroles_txt_mv	Yanjun Mu @@aut@@ Wenping Gong @@aut@@ Yanmin Qie @@aut@@ Xueqing Liu @@aut@@ Linzhi Li @@aut@@ Nina Sun @@aut@@ Wei Liu @@aut@@ Jun Guo @@aut@@ Ran Han @@aut@@ Ziyang Yu @@aut@@ Luning Xiao @@aut@@ Fuyu Su @@aut@@ Wenjing Zhang @@aut@@ Jiangchun Wang @@aut@@ Guohao Han @@aut@@ Pengtao Ma @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	662359240
id	DOAJ084960442
language_de	englisch
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callnumber-first	S - Agriculture
author	Yanjun Mu
spellingShingle	Yanjun Mu misc SB1-1110 misc wheat powdery mildew misc pmYN99102 misc MAS misc BSE-Seq misc molecular mapping misc Plant culture Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing
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topic_title	SB1-1110 Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing wheat powdery mildew pmYN99102 MAS BSE-Seq molecular mapping
topic	misc SB1-1110 misc wheat powdery mildew misc pmYN99102 misc MAS misc BSE-Seq misc molecular mapping misc Plant culture
topic_unstemmed	misc SB1-1110 misc wheat powdery mildew misc pmYN99102 misc MAS misc BSE-Seq misc molecular mapping misc Plant culture
topic_browse	misc SB1-1110 misc wheat powdery mildew misc pmYN99102 misc MAS misc BSE-Seq misc molecular mapping misc Plant culture
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title	Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing
ctrlnum	(DE-627)DOAJ084960442 (DE-599)DOAJb38fe46f282d4119b08337b8d1841e96
title_full	Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing
author_sort	Yanjun Mu
journal	Frontiers in Plant Science
journalStr	Frontiers in Plant Science
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author_browse	Yanjun Mu Wenping Gong Yanmin Qie Xueqing Liu Linzhi Li Nina Sun Wei Liu Jun Guo Ran Han Ziyang Yu Luning Xiao Fuyu Su Wenjing Zhang Jiangchun Wang Guohao Han Pengtao Ma
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author-letter	Yanjun Mu
doi_str_mv	10.3389/fpls.2022.1005627
author2-role	verfasserin
title_sort	identification of the powdery mildew resistance gene in wheat breeding line yannong 99102-06188 via bulked segregant exome capture sequencing
callnumber	SB1-1110
title_auth	Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing
abstract	Powdery mildew of wheat (Triticum aestivum), caused by Blumeria graminis f.sp. tritici (Bgt), is a destructive disease that seriously threatens the yield and quality of its host. Identifying resistance genes is the most attractive and effective strategy for developing disease-resistant cultivars and controlling this disease. In this study, a wheat breeding line Yannong 99102-06188 (YN99102), an elite derivative line from the same breeding process as the famous wheat cultivar Yannong 999, showed high resistance to powdery mildew at the whole growth stages. Genetic analysis was carried out using Bgt isolate E09 and a population of YN99102 crossed with a susceptible parent Jinhe 13–205 (JH13–205). The result indicated that a single recessive gene, tentatively designated pmYN99102, conferred seedling resistance to the Bgt isolate E09. Using bulked segregant exome capture sequencing (BSE-Seq), pmYN99102 was physically located to a ~33.7 Mb (691.0–724.7 Mb) interval on the chromosome arm 2BL, and this interval was further locked in a 1.5 cM genetic interval using molecular markers, which was aligned to a 9.0 Mb physical interval (699.2–708.2 Mb). Based on the analysis of physical location, origin, resistant spectrum, and inherited pattern, pmYN99102 differed from those of the reported powdery mildew (Pm) resistance genes on 2BL, suggesting pmYN99102 is most likely a new Pm gene/allele in the targeted interval. To transfer pmYN99102 to different genetic backgrounds using marker-assisted selection (MAS), 18 closely linked markers were tested for their availability in different genetic backgrounds for MAS, and all markers expect for YTU103-97 can be used in MAS for tracking pmYN99102 when it transferred into those susceptible cultivars.
abstractGer	Powdery mildew of wheat (Triticum aestivum), caused by Blumeria graminis f.sp. tritici (Bgt), is a destructive disease that seriously threatens the yield and quality of its host. Identifying resistance genes is the most attractive and effective strategy for developing disease-resistant cultivars and controlling this disease. In this study, a wheat breeding line Yannong 99102-06188 (YN99102), an elite derivative line from the same breeding process as the famous wheat cultivar Yannong 999, showed high resistance to powdery mildew at the whole growth stages. Genetic analysis was carried out using Bgt isolate E09 and a population of YN99102 crossed with a susceptible parent Jinhe 13–205 (JH13–205). The result indicated that a single recessive gene, tentatively designated pmYN99102, conferred seedling resistance to the Bgt isolate E09. Using bulked segregant exome capture sequencing (BSE-Seq), pmYN99102 was physically located to a ~33.7 Mb (691.0–724.7 Mb) interval on the chromosome arm 2BL, and this interval was further locked in a 1.5 cM genetic interval using molecular markers, which was aligned to a 9.0 Mb physical interval (699.2–708.2 Mb). Based on the analysis of physical location, origin, resistant spectrum, and inherited pattern, pmYN99102 differed from those of the reported powdery mildew (Pm) resistance genes on 2BL, suggesting pmYN99102 is most likely a new Pm gene/allele in the targeted interval. To transfer pmYN99102 to different genetic backgrounds using marker-assisted selection (MAS), 18 closely linked markers were tested for their availability in different genetic backgrounds for MAS, and all markers expect for YTU103-97 can be used in MAS for tracking pmYN99102 when it transferred into those susceptible cultivars.
abstract_unstemmed	Powdery mildew of wheat (Triticum aestivum), caused by Blumeria graminis f.sp. tritici (Bgt), is a destructive disease that seriously threatens the yield and quality of its host. Identifying resistance genes is the most attractive and effective strategy for developing disease-resistant cultivars and controlling this disease. In this study, a wheat breeding line Yannong 99102-06188 (YN99102), an elite derivative line from the same breeding process as the famous wheat cultivar Yannong 999, showed high resistance to powdery mildew at the whole growth stages. Genetic analysis was carried out using Bgt isolate E09 and a population of YN99102 crossed with a susceptible parent Jinhe 13–205 (JH13–205). The result indicated that a single recessive gene, tentatively designated pmYN99102, conferred seedling resistance to the Bgt isolate E09. Using bulked segregant exome capture sequencing (BSE-Seq), pmYN99102 was physically located to a ~33.7 Mb (691.0–724.7 Mb) interval on the chromosome arm 2BL, and this interval was further locked in a 1.5 cM genetic interval using molecular markers, which was aligned to a 9.0 Mb physical interval (699.2–708.2 Mb). Based on the analysis of physical location, origin, resistant spectrum, and inherited pattern, pmYN99102 differed from those of the reported powdery mildew (Pm) resistance genes on 2BL, suggesting pmYN99102 is most likely a new Pm gene/allele in the targeted interval. To transfer pmYN99102 to different genetic backgrounds using marker-assisted selection (MAS), 18 closely linked markers were tested for their availability in different genetic backgrounds for MAS, and all markers expect for YTU103-97 can be used in MAS for tracking pmYN99102 when it transferred into those susceptible cultivars.
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title_short	Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing
url	https://doi.org/10.3389/fpls.2022.1005627 https://doaj.org/article/b38fe46f282d4119b08337b8d1841e96 https://www.frontiersin.org/articles/10.3389/fpls.2022.1005627/full https://doaj.org/toc/1664-462X
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author2	Wenping Gong Yanmin Qie Xueqing Liu Linzhi Li Nina Sun Wei Liu Jun Guo Ran Han Ziyang Yu Luning Xiao Fuyu Su Wenjing Zhang Jiangchun Wang Guohao Han Pengtao Ma
author2Str	Wenping Gong Yanmin Qie Xueqing Liu Linzhi Li Nina Sun Wei Liu Jun Guo Ran Han Ziyang Yu Luning Xiao Fuyu Su Wenjing Zhang Jiangchun Wang Guohao Han Pengtao Ma
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up_date	2024-07-04T01:16:20.062Z
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Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing

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