Long-Term Incubation PrP<sup<CWD</sup< with Soils Affects Prion Recovery but Not Infectivity

Chronic wasting disease (CWD) is a contagious prion disease of cervids. The infectious agent is shed from animals at the preclinical and clinical stages of disease where it persists in the environment as a reservoir of CWD infectivity. In this study, we demonstrate that long-term incubation of CWD p...
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Autor*in:	Alsu Kuznetsova [verfasserIn] Debbie McKenzie [verfasserIn] Catherine Cullingham [verfasserIn] Judd M. Aiken [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	prion protein soil CWD prolonged incubation CWD infectivity prion detection

Übergeordnetes Werk:	In: Pathogens - MDPI AG, 2012, 9(2020), 4, p 311
Übergeordnetes Werk:	volume:9 ; year:2020 ; number:4, p 311

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/pathogens9040311

Katalog-ID:	DOAJ085336408

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520			\|a Chronic wasting disease (CWD) is a contagious prion disease of cervids. The infectious agent is shed from animals at the preclinical and clinical stages of disease where it persists in the environment as a reservoir of CWD infectivity. In this study, we demonstrate that long-term incubation of CWD prions (generated from tg-mice infected with deer or elk prions) with illite, montmorillonite (Mte) and whole soils results in decreased recovery of PrP<sup<CWD</sup<, suggesting that binding becomes more avid and irreversible with time. This continual decline of immunoblot PrP<sup<CWD</sup< detection did not correlate with prion infectivity levels. Bioassay showed no significant differences in incubation periods between mice inoculated with 1% CWD brain homogenate (BH) and with the CWD-BH pre-incubated with quartz or Luvisolic Ae horizon for 1 or 30 weeks. After 55 weeks incubation with Chernozem and Luvisol, bound PrP<sup<CWD</sup< was not detectable by immunoblotting but remained infectious. This study shows that although recovery of PrP<sup<CWD</sup< bound to soil minerals and whole soils with time become more difficult, prion infectivity is not significantly altered. Detection of prions in soil is, therefore, not only affected by soil type but also by length of time of the prion–soil interaction.
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spelling	10.3390/pathogens9040311 doi (DE-627)DOAJ085336408 (DE-599)DOAJcd3eb000402140e8be42bf12c11124f6 DE-627 ger DE-627 rakwb eng Alsu Kuznetsova verfasserin aut Long-Term Incubation PrP<sup<CWD</sup< with Soils Affects Prion Recovery but Not Infectivity 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chronic wasting disease (CWD) is a contagious prion disease of cervids. The infectious agent is shed from animals at the preclinical and clinical stages of disease where it persists in the environment as a reservoir of CWD infectivity. In this study, we demonstrate that long-term incubation of CWD prions (generated from tg-mice infected with deer or elk prions) with illite, montmorillonite (Mte) and whole soils results in decreased recovery of PrP<sup<CWD</sup<, suggesting that binding becomes more avid and irreversible with time. This continual decline of immunoblot PrP<sup<CWD</sup< detection did not correlate with prion infectivity levels. Bioassay showed no significant differences in incubation periods between mice inoculated with 1% CWD brain homogenate (BH) and with the CWD-BH pre-incubated with quartz or Luvisolic Ae horizon for 1 or 30 weeks. After 55 weeks incubation with Chernozem and Luvisol, bound PrP<sup<CWD</sup< was not detectable by immunoblotting but remained infectious. This study shows that although recovery of PrP<sup<CWD</sup< bound to soil minerals and whole soils with time become more difficult, prion infectivity is not significantly altered. Detection of prions in soil is, therefore, not only affected by soil type but also by length of time of the prion–soil interaction. prion protein soil CWD prolonged incubation CWD infectivity prion detection Medicine R Debbie McKenzie verfasserin aut Catherine Cullingham verfasserin aut Judd M. Aiken verfasserin aut In Pathogens MDPI AG, 2012 9(2020), 4, p 311 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:9 year:2020 number:4, p 311 https://doi.org/10.3390/pathogens9040311 kostenfrei https://doaj.org/article/cd3eb000402140e8be42bf12c11124f6 kostenfrei https://www.mdpi.com/2076-0817/9/4/311 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 4, p 311
allfields_unstemmed	10.3390/pathogens9040311 doi (DE-627)DOAJ085336408 (DE-599)DOAJcd3eb000402140e8be42bf12c11124f6 DE-627 ger DE-627 rakwb eng Alsu Kuznetsova verfasserin aut Long-Term Incubation PrP<sup<CWD</sup< with Soils Affects Prion Recovery but Not Infectivity 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chronic wasting disease (CWD) is a contagious prion disease of cervids. The infectious agent is shed from animals at the preclinical and clinical stages of disease where it persists in the environment as a reservoir of CWD infectivity. In this study, we demonstrate that long-term incubation of CWD prions (generated from tg-mice infected with deer or elk prions) with illite, montmorillonite (Mte) and whole soils results in decreased recovery of PrP<sup<CWD</sup<, suggesting that binding becomes more avid and irreversible with time. This continual decline of immunoblot PrP<sup<CWD</sup< detection did not correlate with prion infectivity levels. Bioassay showed no significant differences in incubation periods between mice inoculated with 1% CWD brain homogenate (BH) and with the CWD-BH pre-incubated with quartz or Luvisolic Ae horizon for 1 or 30 weeks. After 55 weeks incubation with Chernozem and Luvisol, bound PrP<sup<CWD</sup< was not detectable by immunoblotting but remained infectious. This study shows that although recovery of PrP<sup<CWD</sup< bound to soil minerals and whole soils with time become more difficult, prion infectivity is not significantly altered. Detection of prions in soil is, therefore, not only affected by soil type but also by length of time of the prion–soil interaction. prion protein soil CWD prolonged incubation CWD infectivity prion detection Medicine R Debbie McKenzie verfasserin aut Catherine Cullingham verfasserin aut Judd M. Aiken verfasserin aut In Pathogens MDPI AG, 2012 9(2020), 4, p 311 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:9 year:2020 number:4, p 311 https://doi.org/10.3390/pathogens9040311 kostenfrei https://doaj.org/article/cd3eb000402140e8be42bf12c11124f6 kostenfrei https://www.mdpi.com/2076-0817/9/4/311 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 4, p 311
allfieldsGer	10.3390/pathogens9040311 doi (DE-627)DOAJ085336408 (DE-599)DOAJcd3eb000402140e8be42bf12c11124f6 DE-627 ger DE-627 rakwb eng Alsu Kuznetsova verfasserin aut Long-Term Incubation PrP<sup<CWD</sup< with Soils Affects Prion Recovery but Not Infectivity 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chronic wasting disease (CWD) is a contagious prion disease of cervids. The infectious agent is shed from animals at the preclinical and clinical stages of disease where it persists in the environment as a reservoir of CWD infectivity. In this study, we demonstrate that long-term incubation of CWD prions (generated from tg-mice infected with deer or elk prions) with illite, montmorillonite (Mte) and whole soils results in decreased recovery of PrP<sup<CWD</sup<, suggesting that binding becomes more avid and irreversible with time. This continual decline of immunoblot PrP<sup<CWD</sup< detection did not correlate with prion infectivity levels. Bioassay showed no significant differences in incubation periods between mice inoculated with 1% CWD brain homogenate (BH) and with the CWD-BH pre-incubated with quartz or Luvisolic Ae horizon for 1 or 30 weeks. After 55 weeks incubation with Chernozem and Luvisol, bound PrP<sup<CWD</sup< was not detectable by immunoblotting but remained infectious. This study shows that although recovery of PrP<sup<CWD</sup< bound to soil minerals and whole soils with time become more difficult, prion infectivity is not significantly altered. Detection of prions in soil is, therefore, not only affected by soil type but also by length of time of the prion–soil interaction. prion protein soil CWD prolonged incubation CWD infectivity prion detection Medicine R Debbie McKenzie verfasserin aut Catherine Cullingham verfasserin aut Judd M. Aiken verfasserin aut In Pathogens MDPI AG, 2012 9(2020), 4, p 311 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:9 year:2020 number:4, p 311 https://doi.org/10.3390/pathogens9040311 kostenfrei https://doaj.org/article/cd3eb000402140e8be42bf12c11124f6 kostenfrei https://www.mdpi.com/2076-0817/9/4/311 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 4, p 311
allfieldsSound	10.3390/pathogens9040311 doi (DE-627)DOAJ085336408 (DE-599)DOAJcd3eb000402140e8be42bf12c11124f6 DE-627 ger DE-627 rakwb eng Alsu Kuznetsova verfasserin aut Long-Term Incubation PrP<sup<CWD</sup< with Soils Affects Prion Recovery but Not Infectivity 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chronic wasting disease (CWD) is a contagious prion disease of cervids. The infectious agent is shed from animals at the preclinical and clinical stages of disease where it persists in the environment as a reservoir of CWD infectivity. In this study, we demonstrate that long-term incubation of CWD prions (generated from tg-mice infected with deer or elk prions) with illite, montmorillonite (Mte) and whole soils results in decreased recovery of PrP<sup<CWD</sup<, suggesting that binding becomes more avid and irreversible with time. This continual decline of immunoblot PrP<sup<CWD</sup< detection did not correlate with prion infectivity levels. Bioassay showed no significant differences in incubation periods between mice inoculated with 1% CWD brain homogenate (BH) and with the CWD-BH pre-incubated with quartz or Luvisolic Ae horizon for 1 or 30 weeks. After 55 weeks incubation with Chernozem and Luvisol, bound PrP<sup<CWD</sup< was not detectable by immunoblotting but remained infectious. This study shows that although recovery of PrP<sup<CWD</sup< bound to soil minerals and whole soils with time become more difficult, prion infectivity is not significantly altered. Detection of prions in soil is, therefore, not only affected by soil type but also by length of time of the prion–soil interaction. prion protein soil CWD prolonged incubation CWD infectivity prion detection Medicine R Debbie McKenzie verfasserin aut Catherine Cullingham verfasserin aut Judd M. Aiken verfasserin aut In Pathogens MDPI AG, 2012 9(2020), 4, p 311 (DE-627)732627885 (DE-600)2695572-6 20760817 nnns volume:9 year:2020 number:4, p 311 https://doi.org/10.3390/pathogens9040311 kostenfrei https://doaj.org/article/cd3eb000402140e8be42bf12c11124f6 kostenfrei https://www.mdpi.com/2076-0817/9/4/311 kostenfrei https://doaj.org/toc/2076-0817 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 4, p 311
language	English
source	In Pathogens 9(2020), 4, p 311 volume:9 year:2020 number:4, p 311
sourceStr	In Pathogens 9(2020), 4, p 311 volume:9 year:2020 number:4, p 311
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institution	findex.gbv.de
topic_facet	prion protein soil CWD prolonged incubation CWD infectivity prion detection Medicine R
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container_title	Pathogens
authorswithroles_txt_mv	Alsu Kuznetsova @@aut@@ Debbie McKenzie @@aut@@ Catherine Cullingham @@aut@@ Judd M. Aiken @@aut@@
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author	Alsu Kuznetsova
spellingShingle	Alsu Kuznetsova misc prion protein misc soil misc CWD misc prolonged incubation misc CWD infectivity misc prion detection misc Medicine misc R Long-Term Incubation PrP<sup<CWD</sup< with Soils Affects Prion Recovery but Not Infectivity
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topic_title	Long-Term Incubation PrP<sup<CWD</sup< with Soils Affects Prion Recovery but Not Infectivity prion protein soil CWD prolonged incubation CWD infectivity prion detection
topic	misc prion protein misc soil misc CWD misc prolonged incubation misc CWD infectivity misc prion detection misc Medicine misc R
topic_unstemmed	misc prion protein misc soil misc CWD misc prolonged incubation misc CWD infectivity misc prion detection misc Medicine misc R
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title	Long-Term Incubation PrP<sup<CWD</sup< with Soils Affects Prion Recovery but Not Infectivity
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title_full	Long-Term Incubation PrP<sup<CWD</sup< with Soils Affects Prion Recovery but Not Infectivity
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title_sort	long-term incubation prp<sup<cwd</sup< with soils affects prion recovery but not infectivity
title_auth	Long-Term Incubation PrP<sup<CWD</sup< with Soils Affects Prion Recovery but Not Infectivity
abstract	Chronic wasting disease (CWD) is a contagious prion disease of cervids. The infectious agent is shed from animals at the preclinical and clinical stages of disease where it persists in the environment as a reservoir of CWD infectivity. In this study, we demonstrate that long-term incubation of CWD prions (generated from tg-mice infected with deer or elk prions) with illite, montmorillonite (Mte) and whole soils results in decreased recovery of PrP<sup<CWD</sup<, suggesting that binding becomes more avid and irreversible with time. This continual decline of immunoblot PrP<sup<CWD</sup< detection did not correlate with prion infectivity levels. Bioassay showed no significant differences in incubation periods between mice inoculated with 1% CWD brain homogenate (BH) and with the CWD-BH pre-incubated with quartz or Luvisolic Ae horizon for 1 or 30 weeks. After 55 weeks incubation with Chernozem and Luvisol, bound PrP<sup<CWD</sup< was not detectable by immunoblotting but remained infectious. This study shows that although recovery of PrP<sup<CWD</sup< bound to soil minerals and whole soils with time become more difficult, prion infectivity is not significantly altered. Detection of prions in soil is, therefore, not only affected by soil type but also by length of time of the prion–soil interaction.
abstractGer	Chronic wasting disease (CWD) is a contagious prion disease of cervids. The infectious agent is shed from animals at the preclinical and clinical stages of disease where it persists in the environment as a reservoir of CWD infectivity. In this study, we demonstrate that long-term incubation of CWD prions (generated from tg-mice infected with deer or elk prions) with illite, montmorillonite (Mte) and whole soils results in decreased recovery of PrP<sup<CWD</sup<, suggesting that binding becomes more avid and irreversible with time. This continual decline of immunoblot PrP<sup<CWD</sup< detection did not correlate with prion infectivity levels. Bioassay showed no significant differences in incubation periods between mice inoculated with 1% CWD brain homogenate (BH) and with the CWD-BH pre-incubated with quartz or Luvisolic Ae horizon for 1 or 30 weeks. After 55 weeks incubation with Chernozem and Luvisol, bound PrP<sup<CWD</sup< was not detectable by immunoblotting but remained infectious. This study shows that although recovery of PrP<sup<CWD</sup< bound to soil minerals and whole soils with time become more difficult, prion infectivity is not significantly altered. Detection of prions in soil is, therefore, not only affected by soil type but also by length of time of the prion–soil interaction.
abstract_unstemmed	Chronic wasting disease (CWD) is a contagious prion disease of cervids. The infectious agent is shed from animals at the preclinical and clinical stages of disease where it persists in the environment as a reservoir of CWD infectivity. In this study, we demonstrate that long-term incubation of CWD prions (generated from tg-mice infected with deer or elk prions) with illite, montmorillonite (Mte) and whole soils results in decreased recovery of PrP<sup<CWD</sup<, suggesting that binding becomes more avid and irreversible with time. This continual decline of immunoblot PrP<sup<CWD</sup< detection did not correlate with prion infectivity levels. Bioassay showed no significant differences in incubation periods between mice inoculated with 1% CWD brain homogenate (BH) and with the CWD-BH pre-incubated with quartz or Luvisolic Ae horizon for 1 or 30 weeks. After 55 weeks incubation with Chernozem and Luvisol, bound PrP<sup<CWD</sup< was not detectable by immunoblotting but remained infectious. This study shows that although recovery of PrP<sup<CWD</sup< bound to soil minerals and whole soils with time become more difficult, prion infectivity is not significantly altered. Detection of prions in soil is, therefore, not only affected by soil type but also by length of time of the prion–soil interaction.
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title_short	Long-Term Incubation PrP<sup<CWD</sup< with Soils Affects Prion Recovery but Not Infectivity
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Long-Term Incubation PrP<sup<CWD</sup< with Soils Affects Prion Recovery but Not Infectivity

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