Identification and Characterization of Carboxylesterases from Brachypodium distachyon Deacetylating Trichothecene Mycotoxins

Increasing frequencies of 3-acetyl-deoxynivalenol (3-ADON)-producing strains of Fusarium graminearum (3-ADON chemotype) have been reported in North America and Asia. 3-ADON is nearly nontoxic at the level of the ribosomal target and has to be deacetylated to cause inhibition of protein biosynthesis....
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Autor*in:	Clemens Schmeitzl [verfasserIn] Elisabeth Varga [verfasserIn] Benedikt Warth [verfasserIn] Karl G. Kugler [verfasserIn] Alexandra Malachová [verfasserIn] Herbert Michlmayr [verfasserIn] Gerlinde Wiesenberger [verfasserIn] Klaus F. X. Mayer [verfasserIn] Hans-Werner Mewes [verfasserIn] Rudolf Krska [verfasserIn] Rainer Schuhmacher [verfasserIn] Franz Berthiller [verfasserIn] Gerhard Adam [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	Fusarium graminearum trichothecene metabolism 3-acetyl-deoxynivalenol 15-acetyl-deoxynivalenol monocot enzymatic cleavage

Übergeordnetes Werk:	In: Toxins - MDPI AG, 2010, 8(2015), 1, p 6
Übergeordnetes Werk:	volume:8 ; year:2015 ; number:1, p 6

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/toxins8010006

Katalog-ID:	DOAJ085358819

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allfields	10.3390/toxins8010006 doi (DE-627)DOAJ085358819 (DE-599)DOAJ3f30044d17cf4c4bb26d89e5dbe51768 DE-627 ger DE-627 rakwb eng Clemens Schmeitzl verfasserin aut Identification and Characterization of Carboxylesterases from Brachypodium distachyon Deacetylating Trichothecene Mycotoxins 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Increasing frequencies of 3-acetyl-deoxynivalenol (3-ADON)-producing strains of Fusarium graminearum (3-ADON chemotype) have been reported in North America and Asia. 3-ADON is nearly nontoxic at the level of the ribosomal target and has to be deacetylated to cause inhibition of protein biosynthesis. Plant cells can efficiently remove the acetyl groups of 3-ADON, but the underlying genes are yet unknown. We therefore performed a study of the family of candidate carboxylesterases (CXE) genes of the monocot model plant Brachypodium distachyon. We report the identification and characterization of the first plant enzymes responsible for deacetylation of trichothecene toxins. The product of the BdCXE29 gene efficiently deacetylates T-2 toxin to HT-2 toxin, NX-2 to NX-3, both 3-ADON and 15-acetyl-deoxynivalenol (15-ADON) into deoxynivalenol and, to a lesser degree, also fusarenon X into nivalenol. The BdCXE52 esterase showed lower activity than BdCXE29 when expressed in yeast and accepts 3-ADON, NX-2, 15-ADON and, to a limited extent, fusarenon X as substrates. Expression of these Brachypodium genes in yeast increases the toxicity of 3-ADON, suggesting that highly similar genes existing in crop plants may act as susceptibility factors in Fusarium head blight disease. Fusarium graminearum trichothecene metabolism 3-acetyl-deoxynivalenol 15-acetyl-deoxynivalenol monocot enzymatic cleavage Medicine R Elisabeth Varga verfasserin aut Benedikt Warth verfasserin aut Karl G. Kugler verfasserin aut Alexandra Malachová verfasserin aut Herbert Michlmayr verfasserin aut Gerlinde Wiesenberger verfasserin aut Klaus F. X. Mayer verfasserin aut Hans-Werner Mewes verfasserin aut Rudolf Krska verfasserin aut Rainer Schuhmacher verfasserin aut Franz Berthiller verfasserin aut Gerhard Adam verfasserin aut In Toxins MDPI AG, 2010 8(2015), 1, p 6 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:8 year:2015 number:1, p 6 https://doi.org/10.3390/toxins8010006 kostenfrei https://doaj.org/article/3f30044d17cf4c4bb26d89e5dbe51768 kostenfrei http://www.mdpi.com/2072-6651/8/1/6 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2015 1, p 6
spelling	10.3390/toxins8010006 doi (DE-627)DOAJ085358819 (DE-599)DOAJ3f30044d17cf4c4bb26d89e5dbe51768 DE-627 ger DE-627 rakwb eng Clemens Schmeitzl verfasserin aut Identification and Characterization of Carboxylesterases from Brachypodium distachyon Deacetylating Trichothecene Mycotoxins 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Increasing frequencies of 3-acetyl-deoxynivalenol (3-ADON)-producing strains of Fusarium graminearum (3-ADON chemotype) have been reported in North America and Asia. 3-ADON is nearly nontoxic at the level of the ribosomal target and has to be deacetylated to cause inhibition of protein biosynthesis. Plant cells can efficiently remove the acetyl groups of 3-ADON, but the underlying genes are yet unknown. We therefore performed a study of the family of candidate carboxylesterases (CXE) genes of the monocot model plant Brachypodium distachyon. We report the identification and characterization of the first plant enzymes responsible for deacetylation of trichothecene toxins. The product of the BdCXE29 gene efficiently deacetylates T-2 toxin to HT-2 toxin, NX-2 to NX-3, both 3-ADON and 15-acetyl-deoxynivalenol (15-ADON) into deoxynivalenol and, to a lesser degree, also fusarenon X into nivalenol. The BdCXE52 esterase showed lower activity than BdCXE29 when expressed in yeast and accepts 3-ADON, NX-2, 15-ADON and, to a limited extent, fusarenon X as substrates. Expression of these Brachypodium genes in yeast increases the toxicity of 3-ADON, suggesting that highly similar genes existing in crop plants may act as susceptibility factors in Fusarium head blight disease. Fusarium graminearum trichothecene metabolism 3-acetyl-deoxynivalenol 15-acetyl-deoxynivalenol monocot enzymatic cleavage Medicine R Elisabeth Varga verfasserin aut Benedikt Warth verfasserin aut Karl G. Kugler verfasserin aut Alexandra Malachová verfasserin aut Herbert Michlmayr verfasserin aut Gerlinde Wiesenberger verfasserin aut Klaus F. X. Mayer verfasserin aut Hans-Werner Mewes verfasserin aut Rudolf Krska verfasserin aut Rainer Schuhmacher verfasserin aut Franz Berthiller verfasserin aut Gerhard Adam verfasserin aut In Toxins MDPI AG, 2010 8(2015), 1, p 6 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:8 year:2015 number:1, p 6 https://doi.org/10.3390/toxins8010006 kostenfrei https://doaj.org/article/3f30044d17cf4c4bb26d89e5dbe51768 kostenfrei http://www.mdpi.com/2072-6651/8/1/6 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2015 1, p 6
allfields_unstemmed	10.3390/toxins8010006 doi (DE-627)DOAJ085358819 (DE-599)DOAJ3f30044d17cf4c4bb26d89e5dbe51768 DE-627 ger DE-627 rakwb eng Clemens Schmeitzl verfasserin aut Identification and Characterization of Carboxylesterases from Brachypodium distachyon Deacetylating Trichothecene Mycotoxins 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Increasing frequencies of 3-acetyl-deoxynivalenol (3-ADON)-producing strains of Fusarium graminearum (3-ADON chemotype) have been reported in North America and Asia. 3-ADON is nearly nontoxic at the level of the ribosomal target and has to be deacetylated to cause inhibition of protein biosynthesis. Plant cells can efficiently remove the acetyl groups of 3-ADON, but the underlying genes are yet unknown. We therefore performed a study of the family of candidate carboxylesterases (CXE) genes of the monocot model plant Brachypodium distachyon. We report the identification and characterization of the first plant enzymes responsible for deacetylation of trichothecene toxins. The product of the BdCXE29 gene efficiently deacetylates T-2 toxin to HT-2 toxin, NX-2 to NX-3, both 3-ADON and 15-acetyl-deoxynivalenol (15-ADON) into deoxynivalenol and, to a lesser degree, also fusarenon X into nivalenol. The BdCXE52 esterase showed lower activity than BdCXE29 when expressed in yeast and accepts 3-ADON, NX-2, 15-ADON and, to a limited extent, fusarenon X as substrates. Expression of these Brachypodium genes in yeast increases the toxicity of 3-ADON, suggesting that highly similar genes existing in crop plants may act as susceptibility factors in Fusarium head blight disease. Fusarium graminearum trichothecene metabolism 3-acetyl-deoxynivalenol 15-acetyl-deoxynivalenol monocot enzymatic cleavage Medicine R Elisabeth Varga verfasserin aut Benedikt Warth verfasserin aut Karl G. Kugler verfasserin aut Alexandra Malachová verfasserin aut Herbert Michlmayr verfasserin aut Gerlinde Wiesenberger verfasserin aut Klaus F. X. Mayer verfasserin aut Hans-Werner Mewes verfasserin aut Rudolf Krska verfasserin aut Rainer Schuhmacher verfasserin aut Franz Berthiller verfasserin aut Gerhard Adam verfasserin aut In Toxins MDPI AG, 2010 8(2015), 1, p 6 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:8 year:2015 number:1, p 6 https://doi.org/10.3390/toxins8010006 kostenfrei https://doaj.org/article/3f30044d17cf4c4bb26d89e5dbe51768 kostenfrei http://www.mdpi.com/2072-6651/8/1/6 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2015 1, p 6
allfieldsGer	10.3390/toxins8010006 doi (DE-627)DOAJ085358819 (DE-599)DOAJ3f30044d17cf4c4bb26d89e5dbe51768 DE-627 ger DE-627 rakwb eng Clemens Schmeitzl verfasserin aut Identification and Characterization of Carboxylesterases from Brachypodium distachyon Deacetylating Trichothecene Mycotoxins 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Increasing frequencies of 3-acetyl-deoxynivalenol (3-ADON)-producing strains of Fusarium graminearum (3-ADON chemotype) have been reported in North America and Asia. 3-ADON is nearly nontoxic at the level of the ribosomal target and has to be deacetylated to cause inhibition of protein biosynthesis. Plant cells can efficiently remove the acetyl groups of 3-ADON, but the underlying genes are yet unknown. We therefore performed a study of the family of candidate carboxylesterases (CXE) genes of the monocot model plant Brachypodium distachyon. We report the identification and characterization of the first plant enzymes responsible for deacetylation of trichothecene toxins. The product of the BdCXE29 gene efficiently deacetylates T-2 toxin to HT-2 toxin, NX-2 to NX-3, both 3-ADON and 15-acetyl-deoxynivalenol (15-ADON) into deoxynivalenol and, to a lesser degree, also fusarenon X into nivalenol. The BdCXE52 esterase showed lower activity than BdCXE29 when expressed in yeast and accepts 3-ADON, NX-2, 15-ADON and, to a limited extent, fusarenon X as substrates. Expression of these Brachypodium genes in yeast increases the toxicity of 3-ADON, suggesting that highly similar genes existing in crop plants may act as susceptibility factors in Fusarium head blight disease. Fusarium graminearum trichothecene metabolism 3-acetyl-deoxynivalenol 15-acetyl-deoxynivalenol monocot enzymatic cleavage Medicine R Elisabeth Varga verfasserin aut Benedikt Warth verfasserin aut Karl G. Kugler verfasserin aut Alexandra Malachová verfasserin aut Herbert Michlmayr verfasserin aut Gerlinde Wiesenberger verfasserin aut Klaus F. X. Mayer verfasserin aut Hans-Werner Mewes verfasserin aut Rudolf Krska verfasserin aut Rainer Schuhmacher verfasserin aut Franz Berthiller verfasserin aut Gerhard Adam verfasserin aut In Toxins MDPI AG, 2010 8(2015), 1, p 6 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:8 year:2015 number:1, p 6 https://doi.org/10.3390/toxins8010006 kostenfrei https://doaj.org/article/3f30044d17cf4c4bb26d89e5dbe51768 kostenfrei http://www.mdpi.com/2072-6651/8/1/6 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2015 1, p 6
allfieldsSound	10.3390/toxins8010006 doi (DE-627)DOAJ085358819 (DE-599)DOAJ3f30044d17cf4c4bb26d89e5dbe51768 DE-627 ger DE-627 rakwb eng Clemens Schmeitzl verfasserin aut Identification and Characterization of Carboxylesterases from Brachypodium distachyon Deacetylating Trichothecene Mycotoxins 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Increasing frequencies of 3-acetyl-deoxynivalenol (3-ADON)-producing strains of Fusarium graminearum (3-ADON chemotype) have been reported in North America and Asia. 3-ADON is nearly nontoxic at the level of the ribosomal target and has to be deacetylated to cause inhibition of protein biosynthesis. Plant cells can efficiently remove the acetyl groups of 3-ADON, but the underlying genes are yet unknown. We therefore performed a study of the family of candidate carboxylesterases (CXE) genes of the monocot model plant Brachypodium distachyon. We report the identification and characterization of the first plant enzymes responsible for deacetylation of trichothecene toxins. The product of the BdCXE29 gene efficiently deacetylates T-2 toxin to HT-2 toxin, NX-2 to NX-3, both 3-ADON and 15-acetyl-deoxynivalenol (15-ADON) into deoxynivalenol and, to a lesser degree, also fusarenon X into nivalenol. The BdCXE52 esterase showed lower activity than BdCXE29 when expressed in yeast and accepts 3-ADON, NX-2, 15-ADON and, to a limited extent, fusarenon X as substrates. Expression of these Brachypodium genes in yeast increases the toxicity of 3-ADON, suggesting that highly similar genes existing in crop plants may act as susceptibility factors in Fusarium head blight disease. Fusarium graminearum trichothecene metabolism 3-acetyl-deoxynivalenol 15-acetyl-deoxynivalenol monocot enzymatic cleavage Medicine R Elisabeth Varga verfasserin aut Benedikt Warth verfasserin aut Karl G. Kugler verfasserin aut Alexandra Malachová verfasserin aut Herbert Michlmayr verfasserin aut Gerlinde Wiesenberger verfasserin aut Klaus F. X. Mayer verfasserin aut Hans-Werner Mewes verfasserin aut Rudolf Krska verfasserin aut Rainer Schuhmacher verfasserin aut Franz Berthiller verfasserin aut Gerhard Adam verfasserin aut In Toxins MDPI AG, 2010 8(2015), 1, p 6 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:8 year:2015 number:1, p 6 https://doi.org/10.3390/toxins8010006 kostenfrei https://doaj.org/article/3f30044d17cf4c4bb26d89e5dbe51768 kostenfrei http://www.mdpi.com/2072-6651/8/1/6 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2015 1, p 6
language	English
source	In Toxins 8(2015), 1, p 6 volume:8 year:2015 number:1, p 6
sourceStr	In Toxins 8(2015), 1, p 6 volume:8 year:2015 number:1, p 6
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Fusarium graminearum trichothecene metabolism 3-acetyl-deoxynivalenol 15-acetyl-deoxynivalenol monocot enzymatic cleavage Medicine R
isfreeaccess_bool	true
container_title	Toxins
authorswithroles_txt_mv	Clemens Schmeitzl @@aut@@ Elisabeth Varga @@aut@@ Benedikt Warth @@aut@@ Karl G. Kugler @@aut@@ Alexandra Malachová @@aut@@ Herbert Michlmayr @@aut@@ Gerlinde Wiesenberger @@aut@@ Klaus F. X. Mayer @@aut@@ Hans-Werner Mewes @@aut@@ Rudolf Krska @@aut@@ Rainer Schuhmacher @@aut@@ Franz Berthiller @@aut@@ Gerhard Adam @@aut@@
publishDateDaySort_date	2015-01-01T00:00:00Z
hierarchy_top_id	610604236
id	DOAJ085358819
language_de	englisch
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author	Clemens Schmeitzl
spellingShingle	Clemens Schmeitzl misc Fusarium graminearum misc trichothecene metabolism misc 3-acetyl-deoxynivalenol misc 15-acetyl-deoxynivalenol misc monocot misc enzymatic cleavage misc Medicine misc R Identification and Characterization of Carboxylesterases from Brachypodium distachyon Deacetylating Trichothecene Mycotoxins
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topic_title	Identification and Characterization of Carboxylesterases from Brachypodium distachyon Deacetylating Trichothecene Mycotoxins Fusarium graminearum trichothecene metabolism 3-acetyl-deoxynivalenol 15-acetyl-deoxynivalenol monocot enzymatic cleavage
topic	misc Fusarium graminearum misc trichothecene metabolism misc 3-acetyl-deoxynivalenol misc 15-acetyl-deoxynivalenol misc monocot misc enzymatic cleavage misc Medicine misc R
topic_unstemmed	misc Fusarium graminearum misc trichothecene metabolism misc 3-acetyl-deoxynivalenol misc 15-acetyl-deoxynivalenol misc monocot misc enzymatic cleavage misc Medicine misc R
topic_browse	misc Fusarium graminearum misc trichothecene metabolism misc 3-acetyl-deoxynivalenol misc 15-acetyl-deoxynivalenol misc monocot misc enzymatic cleavage misc Medicine misc R
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title	Identification and Characterization of Carboxylesterases from Brachypodium distachyon Deacetylating Trichothecene Mycotoxins
ctrlnum	(DE-627)DOAJ085358819 (DE-599)DOAJ3f30044d17cf4c4bb26d89e5dbe51768
title_full	Identification and Characterization of Carboxylesterases from Brachypodium distachyon Deacetylating Trichothecene Mycotoxins
author_sort	Clemens Schmeitzl
journal	Toxins
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author_browse	Clemens Schmeitzl Elisabeth Varga Benedikt Warth Karl G. Kugler Alexandra Malachová Herbert Michlmayr Gerlinde Wiesenberger Klaus F. X. Mayer Hans-Werner Mewes Rudolf Krska Rainer Schuhmacher Franz Berthiller Gerhard Adam
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author-letter	Clemens Schmeitzl
doi_str_mv	10.3390/toxins8010006
author2-role	verfasserin
title_sort	identification and characterization of carboxylesterases from brachypodium distachyon deacetylating trichothecene mycotoxins
title_auth	Identification and Characterization of Carboxylesterases from Brachypodium distachyon Deacetylating Trichothecene Mycotoxins
abstract	Increasing frequencies of 3-acetyl-deoxynivalenol (3-ADON)-producing strains of Fusarium graminearum (3-ADON chemotype) have been reported in North America and Asia. 3-ADON is nearly nontoxic at the level of the ribosomal target and has to be deacetylated to cause inhibition of protein biosynthesis. Plant cells can efficiently remove the acetyl groups of 3-ADON, but the underlying genes are yet unknown. We therefore performed a study of the family of candidate carboxylesterases (CXE) genes of the monocot model plant Brachypodium distachyon. We report the identification and characterization of the first plant enzymes responsible for deacetylation of trichothecene toxins. The product of the BdCXE29 gene efficiently deacetylates T-2 toxin to HT-2 toxin, NX-2 to NX-3, both 3-ADON and 15-acetyl-deoxynivalenol (15-ADON) into deoxynivalenol and, to a lesser degree, also fusarenon X into nivalenol. The BdCXE52 esterase showed lower activity than BdCXE29 when expressed in yeast and accepts 3-ADON, NX-2, 15-ADON and, to a limited extent, fusarenon X as substrates. Expression of these Brachypodium genes in yeast increases the toxicity of 3-ADON, suggesting that highly similar genes existing in crop plants may act as susceptibility factors in Fusarium head blight disease.
abstractGer	Increasing frequencies of 3-acetyl-deoxynivalenol (3-ADON)-producing strains of Fusarium graminearum (3-ADON chemotype) have been reported in North America and Asia. 3-ADON is nearly nontoxic at the level of the ribosomal target and has to be deacetylated to cause inhibition of protein biosynthesis. Plant cells can efficiently remove the acetyl groups of 3-ADON, but the underlying genes are yet unknown. We therefore performed a study of the family of candidate carboxylesterases (CXE) genes of the monocot model plant Brachypodium distachyon. We report the identification and characterization of the first plant enzymes responsible for deacetylation of trichothecene toxins. The product of the BdCXE29 gene efficiently deacetylates T-2 toxin to HT-2 toxin, NX-2 to NX-3, both 3-ADON and 15-acetyl-deoxynivalenol (15-ADON) into deoxynivalenol and, to a lesser degree, also fusarenon X into nivalenol. The BdCXE52 esterase showed lower activity than BdCXE29 when expressed in yeast and accepts 3-ADON, NX-2, 15-ADON and, to a limited extent, fusarenon X as substrates. Expression of these Brachypodium genes in yeast increases the toxicity of 3-ADON, suggesting that highly similar genes existing in crop plants may act as susceptibility factors in Fusarium head blight disease.
abstract_unstemmed	Increasing frequencies of 3-acetyl-deoxynivalenol (3-ADON)-producing strains of Fusarium graminearum (3-ADON chemotype) have been reported in North America and Asia. 3-ADON is nearly nontoxic at the level of the ribosomal target and has to be deacetylated to cause inhibition of protein biosynthesis. Plant cells can efficiently remove the acetyl groups of 3-ADON, but the underlying genes are yet unknown. We therefore performed a study of the family of candidate carboxylesterases (CXE) genes of the monocot model plant Brachypodium distachyon. We report the identification and characterization of the first plant enzymes responsible for deacetylation of trichothecene toxins. The product of the BdCXE29 gene efficiently deacetylates T-2 toxin to HT-2 toxin, NX-2 to NX-3, both 3-ADON and 15-acetyl-deoxynivalenol (15-ADON) into deoxynivalenol and, to a lesser degree, also fusarenon X into nivalenol. The BdCXE52 esterase showed lower activity than BdCXE29 when expressed in yeast and accepts 3-ADON, NX-2, 15-ADON and, to a limited extent, fusarenon X as substrates. Expression of these Brachypodium genes in yeast increases the toxicity of 3-ADON, suggesting that highly similar genes existing in crop plants may act as susceptibility factors in Fusarium head blight disease.
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container_issue	1, p 6
title_short	Identification and Characterization of Carboxylesterases from Brachypodium distachyon Deacetylating Trichothecene Mycotoxins
url	https://doi.org/10.3390/toxins8010006 https://doaj.org/article/3f30044d17cf4c4bb26d89e5dbe51768 http://www.mdpi.com/2072-6651/8/1/6 https://doaj.org/toc/2072-6651
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author2	Elisabeth Varga Benedikt Warth Karl G. Kugler Alexandra Malachová Herbert Michlmayr Gerlinde Wiesenberger Klaus F. X. Mayer Hans-Werner Mewes Rudolf Krska Rainer Schuhmacher Franz Berthiller Gerhard Adam
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up_date	2024-07-03T14:16:20.282Z
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Identification and Characterization of Carboxylesterases from Brachypodium distachyon Deacetylating Trichothecene Mycotoxins

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Vorhandene Bände

Nicht das Richtige dabei?