Discovery Methodology of Novel Conotoxins from <i<Conus</i< Species

Cone snail venoms provide an ideal resource for neuropharmacological tools and drug candidates discovery, which have become a research hotspot in neuroscience and new drug development. More than 1,000,000 natural peptides are produced by cone snails, but less than 0.1% of the estimated conotoxins ha...
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Autor*in:	Ying Fu [verfasserIn] Cheng Li [verfasserIn] Shuai Dong [verfasserIn] Yong Wu [verfasserIn] Dongting Zhangsun [verfasserIn] Sulan Luo [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	discovery methodology novel conotoxins crude venom purification gene cloning transcriptomics proteomics multi-omics

Übergeordnetes Werk:	In: Marine Drugs - MDPI AG, 2005, 16(2018), 11, p 417
Übergeordnetes Werk:	volume:16 ; year:2018 ; number:11, p 417

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/md16110417

Katalog-ID:	DOAJ085377465

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520			\|a Cone snail venoms provide an ideal resource for neuropharmacological tools and drug candidates discovery, which have become a research hotspot in neuroscience and new drug development. More than 1,000,000 natural peptides are produced by cone snails, but less than 0.1% of the estimated conotoxins has been characterized to date. Hence, the discovery of novel conotoxins from the huge conotoxin resources with high-throughput and sensitive methods becomes a crucial key for the conotoxin-based drug development. In this review, we introduce the discovery methodology of new conotoxins from various <i<Conus</i< species. It focuses on obtaining full N- to C-terminal sequences, regardless of disulfide bond connectivity through crude venom purification, conotoxin precusor gene cloning, venom duct transcriptomics, venom proteomics and multi-omic methods. The protocols, advantages, disadvantages, and developments of different approaches during the last decade are summarized and the promising prospects are discussed as well.
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allfields	10.3390/md16110417 doi (DE-627)DOAJ085377465 (DE-599)DOAJ7f5a47e560ad4cc592cd44bbbced93b8 DE-627 ger DE-627 rakwb eng QH301-705.5 Ying Fu verfasserin aut Discovery Methodology of Novel Conotoxins from <i<Conus</i< Species 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cone snail venoms provide an ideal resource for neuropharmacological tools and drug candidates discovery, which have become a research hotspot in neuroscience and new drug development. More than 1,000,000 natural peptides are produced by cone snails, but less than 0.1% of the estimated conotoxins has been characterized to date. Hence, the discovery of novel conotoxins from the huge conotoxin resources with high-throughput and sensitive methods becomes a crucial key for the conotoxin-based drug development. In this review, we introduce the discovery methodology of new conotoxins from various <i<Conus</i< species. It focuses on obtaining full N- to C-terminal sequences, regardless of disulfide bond connectivity through crude venom purification, conotoxin precusor gene cloning, venom duct transcriptomics, venom proteomics and multi-omic methods. The protocols, advantages, disadvantages, and developments of different approaches during the last decade are summarized and the promising prospects are discussed as well. discovery methodology novel conotoxins crude venom purification gene cloning transcriptomics proteomics multi-omics Biology (General) Cheng Li verfasserin aut Shuai Dong verfasserin aut Yong Wu verfasserin aut Dongting Zhangsun verfasserin aut Sulan Luo verfasserin aut In Marine Drugs MDPI AG, 2005 16(2018), 11, p 417 (DE-627)477992420 (DE-600)2175190-0 16603397 nnns volume:16 year:2018 number:11, p 417 https://doi.org/10.3390/md16110417 kostenfrei https://doaj.org/article/7f5a47e560ad4cc592cd44bbbced93b8 kostenfrei https://www.mdpi.com/1660-3397/16/11/417 kostenfrei https://doaj.org/toc/1660-3397 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2018 11, p 417
spelling	10.3390/md16110417 doi (DE-627)DOAJ085377465 (DE-599)DOAJ7f5a47e560ad4cc592cd44bbbced93b8 DE-627 ger DE-627 rakwb eng QH301-705.5 Ying Fu verfasserin aut Discovery Methodology of Novel Conotoxins from <i<Conus</i< Species 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cone snail venoms provide an ideal resource for neuropharmacological tools and drug candidates discovery, which have become a research hotspot in neuroscience and new drug development. More than 1,000,000 natural peptides are produced by cone snails, but less than 0.1% of the estimated conotoxins has been characterized to date. Hence, the discovery of novel conotoxins from the huge conotoxin resources with high-throughput and sensitive methods becomes a crucial key for the conotoxin-based drug development. In this review, we introduce the discovery methodology of new conotoxins from various <i<Conus</i< species. It focuses on obtaining full N- to C-terminal sequences, regardless of disulfide bond connectivity through crude venom purification, conotoxin precusor gene cloning, venom duct transcriptomics, venom proteomics and multi-omic methods. The protocols, advantages, disadvantages, and developments of different approaches during the last decade are summarized and the promising prospects are discussed as well. discovery methodology novel conotoxins crude venom purification gene cloning transcriptomics proteomics multi-omics Biology (General) Cheng Li verfasserin aut Shuai Dong verfasserin aut Yong Wu verfasserin aut Dongting Zhangsun verfasserin aut Sulan Luo verfasserin aut In Marine Drugs MDPI AG, 2005 16(2018), 11, p 417 (DE-627)477992420 (DE-600)2175190-0 16603397 nnns volume:16 year:2018 number:11, p 417 https://doi.org/10.3390/md16110417 kostenfrei https://doaj.org/article/7f5a47e560ad4cc592cd44bbbced93b8 kostenfrei https://www.mdpi.com/1660-3397/16/11/417 kostenfrei https://doaj.org/toc/1660-3397 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2018 11, p 417
allfields_unstemmed	10.3390/md16110417 doi (DE-627)DOAJ085377465 (DE-599)DOAJ7f5a47e560ad4cc592cd44bbbced93b8 DE-627 ger DE-627 rakwb eng QH301-705.5 Ying Fu verfasserin aut Discovery Methodology of Novel Conotoxins from <i<Conus</i< Species 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cone snail venoms provide an ideal resource for neuropharmacological tools and drug candidates discovery, which have become a research hotspot in neuroscience and new drug development. More than 1,000,000 natural peptides are produced by cone snails, but less than 0.1% of the estimated conotoxins has been characterized to date. Hence, the discovery of novel conotoxins from the huge conotoxin resources with high-throughput and sensitive methods becomes a crucial key for the conotoxin-based drug development. In this review, we introduce the discovery methodology of new conotoxins from various <i<Conus</i< species. It focuses on obtaining full N- to C-terminal sequences, regardless of disulfide bond connectivity through crude venom purification, conotoxin precusor gene cloning, venom duct transcriptomics, venom proteomics and multi-omic methods. The protocols, advantages, disadvantages, and developments of different approaches during the last decade are summarized and the promising prospects are discussed as well. discovery methodology novel conotoxins crude venom purification gene cloning transcriptomics proteomics multi-omics Biology (General) Cheng Li verfasserin aut Shuai Dong verfasserin aut Yong Wu verfasserin aut Dongting Zhangsun verfasserin aut Sulan Luo verfasserin aut In Marine Drugs MDPI AG, 2005 16(2018), 11, p 417 (DE-627)477992420 (DE-600)2175190-0 16603397 nnns volume:16 year:2018 number:11, p 417 https://doi.org/10.3390/md16110417 kostenfrei https://doaj.org/article/7f5a47e560ad4cc592cd44bbbced93b8 kostenfrei https://www.mdpi.com/1660-3397/16/11/417 kostenfrei https://doaj.org/toc/1660-3397 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2018 11, p 417
allfieldsGer	10.3390/md16110417 doi (DE-627)DOAJ085377465 (DE-599)DOAJ7f5a47e560ad4cc592cd44bbbced93b8 DE-627 ger DE-627 rakwb eng QH301-705.5 Ying Fu verfasserin aut Discovery Methodology of Novel Conotoxins from <i<Conus</i< Species 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cone snail venoms provide an ideal resource for neuropharmacological tools and drug candidates discovery, which have become a research hotspot in neuroscience and new drug development. More than 1,000,000 natural peptides are produced by cone snails, but less than 0.1% of the estimated conotoxins has been characterized to date. Hence, the discovery of novel conotoxins from the huge conotoxin resources with high-throughput and sensitive methods becomes a crucial key for the conotoxin-based drug development. In this review, we introduce the discovery methodology of new conotoxins from various <i<Conus</i< species. It focuses on obtaining full N- to C-terminal sequences, regardless of disulfide bond connectivity through crude venom purification, conotoxin precusor gene cloning, venom duct transcriptomics, venom proteomics and multi-omic methods. The protocols, advantages, disadvantages, and developments of different approaches during the last decade are summarized and the promising prospects are discussed as well. discovery methodology novel conotoxins crude venom purification gene cloning transcriptomics proteomics multi-omics Biology (General) Cheng Li verfasserin aut Shuai Dong verfasserin aut Yong Wu verfasserin aut Dongting Zhangsun verfasserin aut Sulan Luo verfasserin aut In Marine Drugs MDPI AG, 2005 16(2018), 11, p 417 (DE-627)477992420 (DE-600)2175190-0 16603397 nnns volume:16 year:2018 number:11, p 417 https://doi.org/10.3390/md16110417 kostenfrei https://doaj.org/article/7f5a47e560ad4cc592cd44bbbced93b8 kostenfrei https://www.mdpi.com/1660-3397/16/11/417 kostenfrei https://doaj.org/toc/1660-3397 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2018 11, p 417
allfieldsSound	10.3390/md16110417 doi (DE-627)DOAJ085377465 (DE-599)DOAJ7f5a47e560ad4cc592cd44bbbced93b8 DE-627 ger DE-627 rakwb eng QH301-705.5 Ying Fu verfasserin aut Discovery Methodology of Novel Conotoxins from <i<Conus</i< Species 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cone snail venoms provide an ideal resource for neuropharmacological tools and drug candidates discovery, which have become a research hotspot in neuroscience and new drug development. More than 1,000,000 natural peptides are produced by cone snails, but less than 0.1% of the estimated conotoxins has been characterized to date. Hence, the discovery of novel conotoxins from the huge conotoxin resources with high-throughput and sensitive methods becomes a crucial key for the conotoxin-based drug development. In this review, we introduce the discovery methodology of new conotoxins from various <i<Conus</i< species. It focuses on obtaining full N- to C-terminal sequences, regardless of disulfide bond connectivity through crude venom purification, conotoxin precusor gene cloning, venom duct transcriptomics, venom proteomics and multi-omic methods. The protocols, advantages, disadvantages, and developments of different approaches during the last decade are summarized and the promising prospects are discussed as well. discovery methodology novel conotoxins crude venom purification gene cloning transcriptomics proteomics multi-omics Biology (General) Cheng Li verfasserin aut Shuai Dong verfasserin aut Yong Wu verfasserin aut Dongting Zhangsun verfasserin aut Sulan Luo verfasserin aut In Marine Drugs MDPI AG, 2005 16(2018), 11, p 417 (DE-627)477992420 (DE-600)2175190-0 16603397 nnns volume:16 year:2018 number:11, p 417 https://doi.org/10.3390/md16110417 kostenfrei https://doaj.org/article/7f5a47e560ad4cc592cd44bbbced93b8 kostenfrei https://www.mdpi.com/1660-3397/16/11/417 kostenfrei https://doaj.org/toc/1660-3397 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2018 11, p 417
language	English
source	In Marine Drugs 16(2018), 11, p 417 volume:16 year:2018 number:11, p 417
sourceStr	In Marine Drugs 16(2018), 11, p 417 volume:16 year:2018 number:11, p 417
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institution	findex.gbv.de
topic_facet	discovery methodology novel conotoxins crude venom purification gene cloning transcriptomics proteomics multi-omics Biology (General)
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container_title	Marine Drugs
authorswithroles_txt_mv	Ying Fu @@aut@@ Cheng Li @@aut@@ Shuai Dong @@aut@@ Yong Wu @@aut@@ Dongting Zhangsun @@aut@@ Sulan Luo @@aut@@
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callnumber-first	Q - Science
author	Ying Fu
spellingShingle	Ying Fu misc QH301-705.5 misc discovery misc methodology misc novel conotoxins misc crude venom purification misc gene cloning misc transcriptomics misc proteomics misc multi-omics misc Biology (General) Discovery Methodology of Novel Conotoxins from <i<Conus</i< Species
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topic_title	QH301-705.5 Discovery Methodology of Novel Conotoxins from <i<Conus</i< Species discovery methodology novel conotoxins crude venom purification gene cloning transcriptomics proteomics multi-omics
topic	misc QH301-705.5 misc discovery misc methodology misc novel conotoxins misc crude venom purification misc gene cloning misc transcriptomics misc proteomics misc multi-omics misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc discovery misc methodology misc novel conotoxins misc crude venom purification misc gene cloning misc transcriptomics misc proteomics misc multi-omics misc Biology (General)
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title	Discovery Methodology of Novel Conotoxins from <i<Conus</i< Species
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title_auth	Discovery Methodology of Novel Conotoxins from <i<Conus</i< Species
abstract	Cone snail venoms provide an ideal resource for neuropharmacological tools and drug candidates discovery, which have become a research hotspot in neuroscience and new drug development. More than 1,000,000 natural peptides are produced by cone snails, but less than 0.1% of the estimated conotoxins has been characterized to date. Hence, the discovery of novel conotoxins from the huge conotoxin resources with high-throughput and sensitive methods becomes a crucial key for the conotoxin-based drug development. In this review, we introduce the discovery methodology of new conotoxins from various <i<Conus</i< species. It focuses on obtaining full N- to C-terminal sequences, regardless of disulfide bond connectivity through crude venom purification, conotoxin precusor gene cloning, venom duct transcriptomics, venom proteomics and multi-omic methods. The protocols, advantages, disadvantages, and developments of different approaches during the last decade are summarized and the promising prospects are discussed as well.
abstractGer	Cone snail venoms provide an ideal resource for neuropharmacological tools and drug candidates discovery, which have become a research hotspot in neuroscience and new drug development. More than 1,000,000 natural peptides are produced by cone snails, but less than 0.1% of the estimated conotoxins has been characterized to date. Hence, the discovery of novel conotoxins from the huge conotoxin resources with high-throughput and sensitive methods becomes a crucial key for the conotoxin-based drug development. In this review, we introduce the discovery methodology of new conotoxins from various <i<Conus</i< species. It focuses on obtaining full N- to C-terminal sequences, regardless of disulfide bond connectivity through crude venom purification, conotoxin precusor gene cloning, venom duct transcriptomics, venom proteomics and multi-omic methods. The protocols, advantages, disadvantages, and developments of different approaches during the last decade are summarized and the promising prospects are discussed as well.
abstract_unstemmed	Cone snail venoms provide an ideal resource for neuropharmacological tools and drug candidates discovery, which have become a research hotspot in neuroscience and new drug development. More than 1,000,000 natural peptides are produced by cone snails, but less than 0.1% of the estimated conotoxins has been characterized to date. Hence, the discovery of novel conotoxins from the huge conotoxin resources with high-throughput and sensitive methods becomes a crucial key for the conotoxin-based drug development. In this review, we introduce the discovery methodology of new conotoxins from various <i<Conus</i< species. It focuses on obtaining full N- to C-terminal sequences, regardless of disulfide bond connectivity through crude venom purification, conotoxin precusor gene cloning, venom duct transcriptomics, venom proteomics and multi-omic methods. The protocols, advantages, disadvantages, and developments of different approaches during the last decade are summarized and the promising prospects are discussed as well.
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title_short	Discovery Methodology of Novel Conotoxins from <i<Conus</i< Species
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