MiR-34 promotes apoptosis of lens epithelial cells in cataract rats via the TGF-β/Smads signaling pathway
OBJECTIVE: To discuss the effect of micro ribonucleic acid (miR)-34 on the lens epithelial cell functions in the cataract rats. MATERIALS AND METHODS: Differentially expressed miRNAs in the lens epithelial cells of the cataract rats were screened out by analyzing microarrays. The lens epithelial cel...
Ausführliche Beschreibung
Autor*in: |
G.-B. Zhang [verfasserIn] Z.-G. Liu [verfasserIn] J. Wang [verfasserIn] W. Fan [verfasserIn] |
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E-Artikel |
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Englisch |
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2020 |
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Übergeordnetes Werk: |
In: European Review for Medical and Pharmacological Sciences - Verduci Editore, 2021, 24(2020) |
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Übergeordnetes Werk: |
volume:24 ; year:2020 |
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DOAJ085405825 |
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520 | |a OBJECTIVE: To discuss the effect of micro ribonucleic acid (miR)-34 on the lens epithelial cell functions in the cataract rats. MATERIALS AND METHODS: Differentially expressed miRNAs in the lens epithelial cells of the cataract rats were screened out by analyzing microarrays. The lens epithelial cells of the cataract rats transfected with miR-34 mimics were set as transfection group. Cells with silenced transforming growth factor-β (TGF-β) using RepSox were regarded as the transfection + inhibitor group, and the cells transfected with NC constituted control group. Relative expressions of miR-34, key genes in the TGF-β/Smads signaling pathway and apoptosis-related proteins [B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), Bcl-2 associated K protein (Bak), caspase-9 and surviving] in the control group, transfection group, and transfection + inhibitor group were detected. The proportions of apoptotic cells in the three groups were determined via flow cytometry. RESULTS: The differentially expressed miRNAs in the lens epithelial cells of the cataract rats included miR-5, miR-128, etc. Among the tested miRNAs, miR-34 presented remarkably downregulated expression [log2 fold change (FC) =-2.11, p=0.000]. After the lens epithelial cells of the cataract rats were transfected with miR-34 mimics, the expression of miR-34 was evidently elevated (p=0.000), while the expressions of TGF-β, Smad1, and Smad3 were significantly up-regulated. Following the treatment with the TGF-β inhibitor RepSox, the expressions of TGF-β, Smad1, and Smad3 were downregulated. After transfection of miR-34 mimics in lens epithelial cells of the cataract rats, upregulated Bax and Bak, downregulated Bcl-2 and surviving, and elevated apoptosis rate were observed. After the TGF-β inhibitor RepSox was added, the expressions of Bax and Bak declined prominently, while those of Bcl-2 and survivin were on the contrary, manifesting a declining cell apoptosis rate. The expression of caspase-9 had no significant change among the three groups. The proportion of apoptotic cells in control group, transfection group, and transfection + inhibitor group was 2.33%, 38.14%, and 16.88%, respectively, displaying differences among the three groups (p=0.002). CONCLUSIONS: MiR-34 can promote the apoptosis in lens epithelial cells of cataract rats via the TGF-β/Smads signaling pathway. | ||
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(DE-627)DOAJ085405825 (DE-599)DOAJf06d041faa484185b2f8fe105ae9b128 DE-627 ger DE-627 rakwb eng RM1-950 G.-B. Zhang verfasserin aut MiR-34 promotes apoptosis of lens epithelial cells in cataract rats via the TGF-β/Smads signaling pathway 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier OBJECTIVE: To discuss the effect of micro ribonucleic acid (miR)-34 on the lens epithelial cell functions in the cataract rats. MATERIALS AND METHODS: Differentially expressed miRNAs in the lens epithelial cells of the cataract rats were screened out by analyzing microarrays. The lens epithelial cells of the cataract rats transfected with miR-34 mimics were set as transfection group. Cells with silenced transforming growth factor-β (TGF-β) using RepSox were regarded as the transfection + inhibitor group, and the cells transfected with NC constituted control group. Relative expressions of miR-34, key genes in the TGF-β/Smads signaling pathway and apoptosis-related proteins [B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), Bcl-2 associated K protein (Bak), caspase-9 and surviving] in the control group, transfection group, and transfection + inhibitor group were detected. The proportions of apoptotic cells in the three groups were determined via flow cytometry. RESULTS: The differentially expressed miRNAs in the lens epithelial cells of the cataract rats included miR-5, miR-128, etc. Among the tested miRNAs, miR-34 presented remarkably downregulated expression [log2 fold change (FC) =-2.11, p=0.000]. After the lens epithelial cells of the cataract rats were transfected with miR-34 mimics, the expression of miR-34 was evidently elevated (p=0.000), while the expressions of TGF-β, Smad1, and Smad3 were significantly up-regulated. Following the treatment with the TGF-β inhibitor RepSox, the expressions of TGF-β, Smad1, and Smad3 were downregulated. After transfection of miR-34 mimics in lens epithelial cells of the cataract rats, upregulated Bax and Bak, downregulated Bcl-2 and surviving, and elevated apoptosis rate were observed. After the TGF-β inhibitor RepSox was added, the expressions of Bax and Bak declined prominently, while those of Bcl-2 and survivin were on the contrary, manifesting a declining cell apoptosis rate. The expression of caspase-9 had no significant change among the three groups. The proportion of apoptotic cells in control group, transfection group, and transfection + inhibitor group was 2.33%, 38.14%, and 16.88%, respectively, displaying differences among the three groups (p=0.002). CONCLUSIONS: MiR-34 can promote the apoptosis in lens epithelial cells of cataract rats via the TGF-β/Smads signaling pathway. Therapeutics. Pharmacology Z.-G. Liu verfasserin aut J. Wang verfasserin aut W. Fan verfasserin aut In European Review for Medical and Pharmacological Sciences Verduci Editore, 2021 24(2020) (DE-627)65427049X (DE-600)2598628-4 22840729 nnns volume:24 year:2020 https://doaj.org/article/f06d041faa484185b2f8fe105ae9b128 kostenfrei https://www.europeanreview.org/wp/wp-content/uploads/3485-3491.pdf kostenfrei https://doaj.org/toc/1128-3602 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2153 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2020 |
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(DE-627)DOAJ085405825 (DE-599)DOAJf06d041faa484185b2f8fe105ae9b128 DE-627 ger DE-627 rakwb eng RM1-950 G.-B. Zhang verfasserin aut MiR-34 promotes apoptosis of lens epithelial cells in cataract rats via the TGF-β/Smads signaling pathway 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier OBJECTIVE: To discuss the effect of micro ribonucleic acid (miR)-34 on the lens epithelial cell functions in the cataract rats. MATERIALS AND METHODS: Differentially expressed miRNAs in the lens epithelial cells of the cataract rats were screened out by analyzing microarrays. The lens epithelial cells of the cataract rats transfected with miR-34 mimics were set as transfection group. Cells with silenced transforming growth factor-β (TGF-β) using RepSox were regarded as the transfection + inhibitor group, and the cells transfected with NC constituted control group. Relative expressions of miR-34, key genes in the TGF-β/Smads signaling pathway and apoptosis-related proteins [B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), Bcl-2 associated K protein (Bak), caspase-9 and surviving] in the control group, transfection group, and transfection + inhibitor group were detected. The proportions of apoptotic cells in the three groups were determined via flow cytometry. RESULTS: The differentially expressed miRNAs in the lens epithelial cells of the cataract rats included miR-5, miR-128, etc. Among the tested miRNAs, miR-34 presented remarkably downregulated expression [log2 fold change (FC) =-2.11, p=0.000]. After the lens epithelial cells of the cataract rats were transfected with miR-34 mimics, the expression of miR-34 was evidently elevated (p=0.000), while the expressions of TGF-β, Smad1, and Smad3 were significantly up-regulated. Following the treatment with the TGF-β inhibitor RepSox, the expressions of TGF-β, Smad1, and Smad3 were downregulated. After transfection of miR-34 mimics in lens epithelial cells of the cataract rats, upregulated Bax and Bak, downregulated Bcl-2 and surviving, and elevated apoptosis rate were observed. After the TGF-β inhibitor RepSox was added, the expressions of Bax and Bak declined prominently, while those of Bcl-2 and survivin were on the contrary, manifesting a declining cell apoptosis rate. The expression of caspase-9 had no significant change among the three groups. The proportion of apoptotic cells in control group, transfection group, and transfection + inhibitor group was 2.33%, 38.14%, and 16.88%, respectively, displaying differences among the three groups (p=0.002). CONCLUSIONS: MiR-34 can promote the apoptosis in lens epithelial cells of cataract rats via the TGF-β/Smads signaling pathway. Therapeutics. Pharmacology Z.-G. Liu verfasserin aut J. Wang verfasserin aut W. Fan verfasserin aut In European Review for Medical and Pharmacological Sciences Verduci Editore, 2021 24(2020) (DE-627)65427049X (DE-600)2598628-4 22840729 nnns volume:24 year:2020 https://doaj.org/article/f06d041faa484185b2f8fe105ae9b128 kostenfrei https://www.europeanreview.org/wp/wp-content/uploads/3485-3491.pdf kostenfrei https://doaj.org/toc/1128-3602 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2153 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2020 |
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(DE-627)DOAJ085405825 (DE-599)DOAJf06d041faa484185b2f8fe105ae9b128 DE-627 ger DE-627 rakwb eng RM1-950 G.-B. Zhang verfasserin aut MiR-34 promotes apoptosis of lens epithelial cells in cataract rats via the TGF-β/Smads signaling pathway 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier OBJECTIVE: To discuss the effect of micro ribonucleic acid (miR)-34 on the lens epithelial cell functions in the cataract rats. MATERIALS AND METHODS: Differentially expressed miRNAs in the lens epithelial cells of the cataract rats were screened out by analyzing microarrays. The lens epithelial cells of the cataract rats transfected with miR-34 mimics were set as transfection group. Cells with silenced transforming growth factor-β (TGF-β) using RepSox were regarded as the transfection + inhibitor group, and the cells transfected with NC constituted control group. Relative expressions of miR-34, key genes in the TGF-β/Smads signaling pathway and apoptosis-related proteins [B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), Bcl-2 associated K protein (Bak), caspase-9 and surviving] in the control group, transfection group, and transfection + inhibitor group were detected. The proportions of apoptotic cells in the three groups were determined via flow cytometry. RESULTS: The differentially expressed miRNAs in the lens epithelial cells of the cataract rats included miR-5, miR-128, etc. Among the tested miRNAs, miR-34 presented remarkably downregulated expression [log2 fold change (FC) =-2.11, p=0.000]. After the lens epithelial cells of the cataract rats were transfected with miR-34 mimics, the expression of miR-34 was evidently elevated (p=0.000), while the expressions of TGF-β, Smad1, and Smad3 were significantly up-regulated. Following the treatment with the TGF-β inhibitor RepSox, the expressions of TGF-β, Smad1, and Smad3 were downregulated. After transfection of miR-34 mimics in lens epithelial cells of the cataract rats, upregulated Bax and Bak, downregulated Bcl-2 and surviving, and elevated apoptosis rate were observed. After the TGF-β inhibitor RepSox was added, the expressions of Bax and Bak declined prominently, while those of Bcl-2 and survivin were on the contrary, manifesting a declining cell apoptosis rate. The expression of caspase-9 had no significant change among the three groups. The proportion of apoptotic cells in control group, transfection group, and transfection + inhibitor group was 2.33%, 38.14%, and 16.88%, respectively, displaying differences among the three groups (p=0.002). CONCLUSIONS: MiR-34 can promote the apoptosis in lens epithelial cells of cataract rats via the TGF-β/Smads signaling pathway. Therapeutics. Pharmacology Z.-G. Liu verfasserin aut J. Wang verfasserin aut W. Fan verfasserin aut In European Review for Medical and Pharmacological Sciences Verduci Editore, 2021 24(2020) (DE-627)65427049X (DE-600)2598628-4 22840729 nnns volume:24 year:2020 https://doaj.org/article/f06d041faa484185b2f8fe105ae9b128 kostenfrei https://www.europeanreview.org/wp/wp-content/uploads/3485-3491.pdf kostenfrei https://doaj.org/toc/1128-3602 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2153 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2020 |
allfieldsGer |
(DE-627)DOAJ085405825 (DE-599)DOAJf06d041faa484185b2f8fe105ae9b128 DE-627 ger DE-627 rakwb eng RM1-950 G.-B. Zhang verfasserin aut MiR-34 promotes apoptosis of lens epithelial cells in cataract rats via the TGF-β/Smads signaling pathway 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier OBJECTIVE: To discuss the effect of micro ribonucleic acid (miR)-34 on the lens epithelial cell functions in the cataract rats. MATERIALS AND METHODS: Differentially expressed miRNAs in the lens epithelial cells of the cataract rats were screened out by analyzing microarrays. The lens epithelial cells of the cataract rats transfected with miR-34 mimics were set as transfection group. Cells with silenced transforming growth factor-β (TGF-β) using RepSox were regarded as the transfection + inhibitor group, and the cells transfected with NC constituted control group. Relative expressions of miR-34, key genes in the TGF-β/Smads signaling pathway and apoptosis-related proteins [B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), Bcl-2 associated K protein (Bak), caspase-9 and surviving] in the control group, transfection group, and transfection + inhibitor group were detected. The proportions of apoptotic cells in the three groups were determined via flow cytometry. RESULTS: The differentially expressed miRNAs in the lens epithelial cells of the cataract rats included miR-5, miR-128, etc. Among the tested miRNAs, miR-34 presented remarkably downregulated expression [log2 fold change (FC) =-2.11, p=0.000]. After the lens epithelial cells of the cataract rats were transfected with miR-34 mimics, the expression of miR-34 was evidently elevated (p=0.000), while the expressions of TGF-β, Smad1, and Smad3 were significantly up-regulated. Following the treatment with the TGF-β inhibitor RepSox, the expressions of TGF-β, Smad1, and Smad3 were downregulated. After transfection of miR-34 mimics in lens epithelial cells of the cataract rats, upregulated Bax and Bak, downregulated Bcl-2 and surviving, and elevated apoptosis rate were observed. After the TGF-β inhibitor RepSox was added, the expressions of Bax and Bak declined prominently, while those of Bcl-2 and survivin were on the contrary, manifesting a declining cell apoptosis rate. The expression of caspase-9 had no significant change among the three groups. The proportion of apoptotic cells in control group, transfection group, and transfection + inhibitor group was 2.33%, 38.14%, and 16.88%, respectively, displaying differences among the three groups (p=0.002). CONCLUSIONS: MiR-34 can promote the apoptosis in lens epithelial cells of cataract rats via the TGF-β/Smads signaling pathway. Therapeutics. Pharmacology Z.-G. Liu verfasserin aut J. Wang verfasserin aut W. Fan verfasserin aut In European Review for Medical and Pharmacological Sciences Verduci Editore, 2021 24(2020) (DE-627)65427049X (DE-600)2598628-4 22840729 nnns volume:24 year:2020 https://doaj.org/article/f06d041faa484185b2f8fe105ae9b128 kostenfrei https://www.europeanreview.org/wp/wp-content/uploads/3485-3491.pdf kostenfrei https://doaj.org/toc/1128-3602 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2153 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2020 |
allfieldsSound |
(DE-627)DOAJ085405825 (DE-599)DOAJf06d041faa484185b2f8fe105ae9b128 DE-627 ger DE-627 rakwb eng RM1-950 G.-B. Zhang verfasserin aut MiR-34 promotes apoptosis of lens epithelial cells in cataract rats via the TGF-β/Smads signaling pathway 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier OBJECTIVE: To discuss the effect of micro ribonucleic acid (miR)-34 on the lens epithelial cell functions in the cataract rats. MATERIALS AND METHODS: Differentially expressed miRNAs in the lens epithelial cells of the cataract rats were screened out by analyzing microarrays. The lens epithelial cells of the cataract rats transfected with miR-34 mimics were set as transfection group. Cells with silenced transforming growth factor-β (TGF-β) using RepSox were regarded as the transfection + inhibitor group, and the cells transfected with NC constituted control group. Relative expressions of miR-34, key genes in the TGF-β/Smads signaling pathway and apoptosis-related proteins [B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), Bcl-2 associated K protein (Bak), caspase-9 and surviving] in the control group, transfection group, and transfection + inhibitor group were detected. The proportions of apoptotic cells in the three groups were determined via flow cytometry. RESULTS: The differentially expressed miRNAs in the lens epithelial cells of the cataract rats included miR-5, miR-128, etc. Among the tested miRNAs, miR-34 presented remarkably downregulated expression [log2 fold change (FC) =-2.11, p=0.000]. After the lens epithelial cells of the cataract rats were transfected with miR-34 mimics, the expression of miR-34 was evidently elevated (p=0.000), while the expressions of TGF-β, Smad1, and Smad3 were significantly up-regulated. Following the treatment with the TGF-β inhibitor RepSox, the expressions of TGF-β, Smad1, and Smad3 were downregulated. After transfection of miR-34 mimics in lens epithelial cells of the cataract rats, upregulated Bax and Bak, downregulated Bcl-2 and surviving, and elevated apoptosis rate were observed. After the TGF-β inhibitor RepSox was added, the expressions of Bax and Bak declined prominently, while those of Bcl-2 and survivin were on the contrary, manifesting a declining cell apoptosis rate. The expression of caspase-9 had no significant change among the three groups. The proportion of apoptotic cells in control group, transfection group, and transfection + inhibitor group was 2.33%, 38.14%, and 16.88%, respectively, displaying differences among the three groups (p=0.002). CONCLUSIONS: MiR-34 can promote the apoptosis in lens epithelial cells of cataract rats via the TGF-β/Smads signaling pathway. Therapeutics. Pharmacology Z.-G. Liu verfasserin aut J. Wang verfasserin aut W. Fan verfasserin aut In European Review for Medical and Pharmacological Sciences Verduci Editore, 2021 24(2020) (DE-627)65427049X (DE-600)2598628-4 22840729 nnns volume:24 year:2020 https://doaj.org/article/f06d041faa484185b2f8fe105ae9b128 kostenfrei https://www.europeanreview.org/wp/wp-content/uploads/3485-3491.pdf kostenfrei https://doaj.org/toc/1128-3602 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2153 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2020 |
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Zhang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">MiR-34 promotes apoptosis of lens epithelial cells in cataract rats via the TGF-β/Smads signaling pathway</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2020</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">OBJECTIVE: To discuss the effect of micro ribonucleic acid (miR)-34 on the lens epithelial cell functions in the cataract rats. MATERIALS AND METHODS: Differentially expressed miRNAs in the lens epithelial cells of the cataract rats were screened out by analyzing microarrays. The lens epithelial cells of the cataract rats transfected with miR-34 mimics were set as transfection group. Cells with silenced transforming growth factor-β (TGF-β) using RepSox were regarded as the transfection + inhibitor group, and the cells transfected with NC constituted control group. Relative expressions of miR-34, key genes in the TGF-β/Smads signaling pathway and apoptosis-related proteins [B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), Bcl-2 associated K protein (Bak), caspase-9 and surviving] in the control group, transfection group, and transfection + inhibitor group were detected. The proportions of apoptotic cells in the three groups were determined via flow cytometry. 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MiR-34 promotes apoptosis of lens epithelial cells in cataract rats via the TGF-β/Smads signaling pathway |
abstract |
OBJECTIVE: To discuss the effect of micro ribonucleic acid (miR)-34 on the lens epithelial cell functions in the cataract rats. MATERIALS AND METHODS: Differentially expressed miRNAs in the lens epithelial cells of the cataract rats were screened out by analyzing microarrays. The lens epithelial cells of the cataract rats transfected with miR-34 mimics were set as transfection group. Cells with silenced transforming growth factor-β (TGF-β) using RepSox were regarded as the transfection + inhibitor group, and the cells transfected with NC constituted control group. Relative expressions of miR-34, key genes in the TGF-β/Smads signaling pathway and apoptosis-related proteins [B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), Bcl-2 associated K protein (Bak), caspase-9 and surviving] in the control group, transfection group, and transfection + inhibitor group were detected. The proportions of apoptotic cells in the three groups were determined via flow cytometry. RESULTS: The differentially expressed miRNAs in the lens epithelial cells of the cataract rats included miR-5, miR-128, etc. Among the tested miRNAs, miR-34 presented remarkably downregulated expression [log2 fold change (FC) =-2.11, p=0.000]. After the lens epithelial cells of the cataract rats were transfected with miR-34 mimics, the expression of miR-34 was evidently elevated (p=0.000), while the expressions of TGF-β, Smad1, and Smad3 were significantly up-regulated. Following the treatment with the TGF-β inhibitor RepSox, the expressions of TGF-β, Smad1, and Smad3 were downregulated. After transfection of miR-34 mimics in lens epithelial cells of the cataract rats, upregulated Bax and Bak, downregulated Bcl-2 and surviving, and elevated apoptosis rate were observed. After the TGF-β inhibitor RepSox was added, the expressions of Bax and Bak declined prominently, while those of Bcl-2 and survivin were on the contrary, manifesting a declining cell apoptosis rate. The expression of caspase-9 had no significant change among the three groups. The proportion of apoptotic cells in control group, transfection group, and transfection + inhibitor group was 2.33%, 38.14%, and 16.88%, respectively, displaying differences among the three groups (p=0.002). CONCLUSIONS: MiR-34 can promote the apoptosis in lens epithelial cells of cataract rats via the TGF-β/Smads signaling pathway. |
abstractGer |
OBJECTIVE: To discuss the effect of micro ribonucleic acid (miR)-34 on the lens epithelial cell functions in the cataract rats. MATERIALS AND METHODS: Differentially expressed miRNAs in the lens epithelial cells of the cataract rats were screened out by analyzing microarrays. The lens epithelial cells of the cataract rats transfected with miR-34 mimics were set as transfection group. Cells with silenced transforming growth factor-β (TGF-β) using RepSox were regarded as the transfection + inhibitor group, and the cells transfected with NC constituted control group. Relative expressions of miR-34, key genes in the TGF-β/Smads signaling pathway and apoptosis-related proteins [B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), Bcl-2 associated K protein (Bak), caspase-9 and surviving] in the control group, transfection group, and transfection + inhibitor group were detected. The proportions of apoptotic cells in the three groups were determined via flow cytometry. RESULTS: The differentially expressed miRNAs in the lens epithelial cells of the cataract rats included miR-5, miR-128, etc. Among the tested miRNAs, miR-34 presented remarkably downregulated expression [log2 fold change (FC) =-2.11, p=0.000]. After the lens epithelial cells of the cataract rats were transfected with miR-34 mimics, the expression of miR-34 was evidently elevated (p=0.000), while the expressions of TGF-β, Smad1, and Smad3 were significantly up-regulated. Following the treatment with the TGF-β inhibitor RepSox, the expressions of TGF-β, Smad1, and Smad3 were downregulated. After transfection of miR-34 mimics in lens epithelial cells of the cataract rats, upregulated Bax and Bak, downregulated Bcl-2 and surviving, and elevated apoptosis rate were observed. After the TGF-β inhibitor RepSox was added, the expressions of Bax and Bak declined prominently, while those of Bcl-2 and survivin were on the contrary, manifesting a declining cell apoptosis rate. The expression of caspase-9 had no significant change among the three groups. The proportion of apoptotic cells in control group, transfection group, and transfection + inhibitor group was 2.33%, 38.14%, and 16.88%, respectively, displaying differences among the three groups (p=0.002). CONCLUSIONS: MiR-34 can promote the apoptosis in lens epithelial cells of cataract rats via the TGF-β/Smads signaling pathway. |
abstract_unstemmed |
OBJECTIVE: To discuss the effect of micro ribonucleic acid (miR)-34 on the lens epithelial cell functions in the cataract rats. MATERIALS AND METHODS: Differentially expressed miRNAs in the lens epithelial cells of the cataract rats were screened out by analyzing microarrays. The lens epithelial cells of the cataract rats transfected with miR-34 mimics were set as transfection group. Cells with silenced transforming growth factor-β (TGF-β) using RepSox were regarded as the transfection + inhibitor group, and the cells transfected with NC constituted control group. Relative expressions of miR-34, key genes in the TGF-β/Smads signaling pathway and apoptosis-related proteins [B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), Bcl-2 associated K protein (Bak), caspase-9 and surviving] in the control group, transfection group, and transfection + inhibitor group were detected. The proportions of apoptotic cells in the three groups were determined via flow cytometry. RESULTS: The differentially expressed miRNAs in the lens epithelial cells of the cataract rats included miR-5, miR-128, etc. Among the tested miRNAs, miR-34 presented remarkably downregulated expression [log2 fold change (FC) =-2.11, p=0.000]. After the lens epithelial cells of the cataract rats were transfected with miR-34 mimics, the expression of miR-34 was evidently elevated (p=0.000), while the expressions of TGF-β, Smad1, and Smad3 were significantly up-regulated. Following the treatment with the TGF-β inhibitor RepSox, the expressions of TGF-β, Smad1, and Smad3 were downregulated. After transfection of miR-34 mimics in lens epithelial cells of the cataract rats, upregulated Bax and Bak, downregulated Bcl-2 and surviving, and elevated apoptosis rate were observed. After the TGF-β inhibitor RepSox was added, the expressions of Bax and Bak declined prominently, while those of Bcl-2 and survivin were on the contrary, manifesting a declining cell apoptosis rate. The expression of caspase-9 had no significant change among the three groups. The proportion of apoptotic cells in control group, transfection group, and transfection + inhibitor group was 2.33%, 38.14%, and 16.88%, respectively, displaying differences among the three groups (p=0.002). CONCLUSIONS: MiR-34 can promote the apoptosis in lens epithelial cells of cataract rats via the TGF-β/Smads signaling pathway. |
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title_short |
MiR-34 promotes apoptosis of lens epithelial cells in cataract rats via the TGF-β/Smads signaling pathway |
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https://doaj.org/article/f06d041faa484185b2f8fe105ae9b128 https://www.europeanreview.org/wp/wp-content/uploads/3485-3491.pdf https://doaj.org/toc/1128-3602 |
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Zhang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">MiR-34 promotes apoptosis of lens epithelial cells in cataract rats via the TGF-β/Smads signaling pathway</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2020</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">OBJECTIVE: To discuss the effect of micro ribonucleic acid (miR)-34 on the lens epithelial cell functions in the cataract rats. MATERIALS AND METHODS: Differentially expressed miRNAs in the lens epithelial cells of the cataract rats were screened out by analyzing microarrays. The lens epithelial cells of the cataract rats transfected with miR-34 mimics were set as transfection group. Cells with silenced transforming growth factor-β (TGF-β) using RepSox were regarded as the transfection + inhibitor group, and the cells transfected with NC constituted control group. Relative expressions of miR-34, key genes in the TGF-β/Smads signaling pathway and apoptosis-related proteins [B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), Bcl-2 associated K protein (Bak), caspase-9 and surviving] in the control group, transfection group, and transfection + inhibitor group were detected. The proportions of apoptotic cells in the three groups were determined via flow cytometry. RESULTS: The differentially expressed miRNAs in the lens epithelial cells of the cataract rats included miR-5, miR-128, etc. Among the tested miRNAs, miR-34 presented remarkably downregulated expression [log2 fold change (FC) =-2.11, p=0.000]. After the lens epithelial cells of the cataract rats were transfected with miR-34 mimics, the expression of miR-34 was evidently elevated (p=0.000), while the expressions of TGF-β, Smad1, and Smad3 were significantly up-regulated. Following the treatment with the TGF-β inhibitor RepSox, the expressions of TGF-β, Smad1, and Smad3 were downregulated. After transfection of miR-34 mimics in lens epithelial cells of the cataract rats, upregulated Bax and Bak, downregulated Bcl-2 and surviving, and elevated apoptosis rate were observed. After the TGF-β inhibitor RepSox was added, the expressions of Bax and Bak declined prominently, while those of Bcl-2 and survivin were on the contrary, manifesting a declining cell apoptosis rate. The expression of caspase-9 had no significant change among the three groups. The proportion of apoptotic cells in control group, transfection group, and transfection + inhibitor group was 2.33%, 38.14%, and 16.88%, respectively, displaying differences among the three groups (p=0.002). CONCLUSIONS: MiR-34 can promote the apoptosis in lens epithelial cells of cataract rats via the TGF-β/Smads signaling pathway.</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Therapeutics. Pharmacology</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Z.-G. Liu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">J. Wang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">W. 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