Identification of a New Antimicrobial, Desertomycin H, Utilizing a Modified Crowded Plate Technique

The antibiotic-resistant bacteria-associated infections are a major global healthcare threat. New classes of antimicrobial compounds are urgently needed as the frequency of infections caused by multidrug-resistant microbes continues to rise. Recent metagenomic data have demonstrated that there is st...
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Autor*in:	Osama G. Mohamed [verfasserIn] Sadaf Dorandish [verfasserIn] Rebecca Lindow [verfasserIn] Megan Steltz [verfasserIn] Ifrah Shoukat [verfasserIn] Maira Shoukat [verfasserIn] Hussein Chehade [verfasserIn] Sara Baghdadi [verfasserIn] Madelaine McAlister-Raeburn [verfasserIn] Asad Kamal [verfasserIn] Dawit Abebe [verfasserIn] Khaled Ali [verfasserIn] Chelsey Ivy [verfasserIn] Maria Antonova [verfasserIn] Pamela Schultz [verfasserIn] Michael Angell [verfasserIn] Daniel Clemans [verfasserIn] Timothy Friebe [verfasserIn] David Sherman [verfasserIn] Anne M. Casper [verfasserIn] Paul A. Price [verfasserIn] Ashootosh Tripathi [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	antibiotic discovery crowded plate technique ESKAPE pathogens natural products Tiny Earth

Übergeordnetes Werk:	In: Marine Drugs - MDPI AG, 2005, 19(2021), 8, p 424
Übergeordnetes Werk:	volume:19 ; year:2021 ; number:8, p 424

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/md19080424

Katalog-ID:	DOAJ085556351

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spelling	10.3390/md19080424 doi (DE-627)DOAJ085556351 (DE-599)DOAJ09f8b4e7cf2946e7962b7c8fafdf8b6b DE-627 ger DE-627 rakwb eng QH301-705.5 Osama G. Mohamed verfasserin aut Identification of a New Antimicrobial, Desertomycin H, Utilizing a Modified Crowded Plate Technique 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The antibiotic-resistant bacteria-associated infections are a major global healthcare threat. New classes of antimicrobial compounds are urgently needed as the frequency of infections caused by multidrug-resistant microbes continues to rise. Recent metagenomic data have demonstrated that there is still biosynthetic potential encoded in but transcriptionally silent in cultivatable bacterial genomes. However, the culture conditions required to identify and express silent biosynthetic gene clusters that yield natural products with antimicrobial activity are largely unknown. Here, we describe a new antibiotic discovery scheme, dubbed the modified crowded plate technique (mCPT), that utilizes complex microbial interactions to elicit antimicrobial production from otherwise silent biosynthetic gene clusters. Using the mCPT as part of the antibiotic crowdsourcing educational program Tiny Earth<sup<TM</sup<, we isolated over 1400 antibiotic-producing microbes, including 62 showing activity against multidrug-resistant pathogens. The natural product extracts generated from six microbial isolates showed potent activity against vancomycin-intermediate resistant <i<Staphylococcus aureus</i<. We utilized a targeted approach that coupled mass spectrometry data with bioactivity, yielding a new macrolactone class of metabolite, desertomycin H. In this study, we successfully demonstrate a concept that significantly increased our ability to quickly and efficiently identify microbes capable of the silent antibiotic production. antibiotic discovery crowded plate technique ESKAPE pathogens natural products Tiny Earth<sup<TM</sup< Biology (General) Sadaf Dorandish verfasserin aut Rebecca Lindow verfasserin aut Megan Steltz verfasserin aut Ifrah Shoukat verfasserin aut Maira Shoukat verfasserin aut Hussein Chehade verfasserin aut Sara Baghdadi verfasserin aut Madelaine McAlister-Raeburn verfasserin aut Asad Kamal verfasserin aut Dawit Abebe verfasserin aut Khaled Ali verfasserin aut Chelsey Ivy verfasserin aut Maria Antonova verfasserin aut Pamela Schultz verfasserin aut Michael Angell verfasserin aut Daniel Clemans verfasserin aut Timothy Friebe verfasserin aut David Sherman verfasserin aut Anne M. Casper verfasserin aut Paul A. Price verfasserin aut Ashootosh Tripathi verfasserin aut In Marine Drugs MDPI AG, 2005 19(2021), 8, p 424 (DE-627)477992420 (DE-600)2175190-0 16603397 nnns volume:19 year:2021 number:8, p 424 https://doi.org/10.3390/md19080424 kostenfrei https://doaj.org/article/09f8b4e7cf2946e7962b7c8fafdf8b6b kostenfrei https://www.mdpi.com/1660-3397/19/8/424 kostenfrei https://doaj.org/toc/1660-3397 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2021 8, p 424
allfields_unstemmed	10.3390/md19080424 doi (DE-627)DOAJ085556351 (DE-599)DOAJ09f8b4e7cf2946e7962b7c8fafdf8b6b DE-627 ger DE-627 rakwb eng QH301-705.5 Osama G. Mohamed verfasserin aut Identification of a New Antimicrobial, Desertomycin H, Utilizing a Modified Crowded Plate Technique 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The antibiotic-resistant bacteria-associated infections are a major global healthcare threat. New classes of antimicrobial compounds are urgently needed as the frequency of infections caused by multidrug-resistant microbes continues to rise. Recent metagenomic data have demonstrated that there is still biosynthetic potential encoded in but transcriptionally silent in cultivatable bacterial genomes. However, the culture conditions required to identify and express silent biosynthetic gene clusters that yield natural products with antimicrobial activity are largely unknown. Here, we describe a new antibiotic discovery scheme, dubbed the modified crowded plate technique (mCPT), that utilizes complex microbial interactions to elicit antimicrobial production from otherwise silent biosynthetic gene clusters. Using the mCPT as part of the antibiotic crowdsourcing educational program Tiny Earth<sup<TM</sup<, we isolated over 1400 antibiotic-producing microbes, including 62 showing activity against multidrug-resistant pathogens. The natural product extracts generated from six microbial isolates showed potent activity against vancomycin-intermediate resistant <i<Staphylococcus aureus</i<. We utilized a targeted approach that coupled mass spectrometry data with bioactivity, yielding a new macrolactone class of metabolite, desertomycin H. In this study, we successfully demonstrate a concept that significantly increased our ability to quickly and efficiently identify microbes capable of the silent antibiotic production. antibiotic discovery crowded plate technique ESKAPE pathogens natural products Tiny Earth<sup<TM</sup< Biology (General) Sadaf Dorandish verfasserin aut Rebecca Lindow verfasserin aut Megan Steltz verfasserin aut Ifrah Shoukat verfasserin aut Maira Shoukat verfasserin aut Hussein Chehade verfasserin aut Sara Baghdadi verfasserin aut Madelaine McAlister-Raeburn verfasserin aut Asad Kamal verfasserin aut Dawit Abebe verfasserin aut Khaled Ali verfasserin aut Chelsey Ivy verfasserin aut Maria Antonova verfasserin aut Pamela Schultz verfasserin aut Michael Angell verfasserin aut Daniel Clemans verfasserin aut Timothy Friebe verfasserin aut David Sherman verfasserin aut Anne M. Casper verfasserin aut Paul A. Price verfasserin aut Ashootosh Tripathi verfasserin aut In Marine Drugs MDPI AG, 2005 19(2021), 8, p 424 (DE-627)477992420 (DE-600)2175190-0 16603397 nnns volume:19 year:2021 number:8, p 424 https://doi.org/10.3390/md19080424 kostenfrei https://doaj.org/article/09f8b4e7cf2946e7962b7c8fafdf8b6b kostenfrei https://www.mdpi.com/1660-3397/19/8/424 kostenfrei https://doaj.org/toc/1660-3397 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2021 8, p 424
allfieldsGer	10.3390/md19080424 doi (DE-627)DOAJ085556351 (DE-599)DOAJ09f8b4e7cf2946e7962b7c8fafdf8b6b DE-627 ger DE-627 rakwb eng QH301-705.5 Osama G. Mohamed verfasserin aut Identification of a New Antimicrobial, Desertomycin H, Utilizing a Modified Crowded Plate Technique 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The antibiotic-resistant bacteria-associated infections are a major global healthcare threat. New classes of antimicrobial compounds are urgently needed as the frequency of infections caused by multidrug-resistant microbes continues to rise. Recent metagenomic data have demonstrated that there is still biosynthetic potential encoded in but transcriptionally silent in cultivatable bacterial genomes. However, the culture conditions required to identify and express silent biosynthetic gene clusters that yield natural products with antimicrobial activity are largely unknown. Here, we describe a new antibiotic discovery scheme, dubbed the modified crowded plate technique (mCPT), that utilizes complex microbial interactions to elicit antimicrobial production from otherwise silent biosynthetic gene clusters. Using the mCPT as part of the antibiotic crowdsourcing educational program Tiny Earth<sup<TM</sup<, we isolated over 1400 antibiotic-producing microbes, including 62 showing activity against multidrug-resistant pathogens. The natural product extracts generated from six microbial isolates showed potent activity against vancomycin-intermediate resistant <i<Staphylococcus aureus</i<. We utilized a targeted approach that coupled mass spectrometry data with bioactivity, yielding a new macrolactone class of metabolite, desertomycin H. In this study, we successfully demonstrate a concept that significantly increased our ability to quickly and efficiently identify microbes capable of the silent antibiotic production. antibiotic discovery crowded plate technique ESKAPE pathogens natural products Tiny Earth<sup<TM</sup< Biology (General) Sadaf Dorandish verfasserin aut Rebecca Lindow verfasserin aut Megan Steltz verfasserin aut Ifrah Shoukat verfasserin aut Maira Shoukat verfasserin aut Hussein Chehade verfasserin aut Sara Baghdadi verfasserin aut Madelaine McAlister-Raeburn verfasserin aut Asad Kamal verfasserin aut Dawit Abebe verfasserin aut Khaled Ali verfasserin aut Chelsey Ivy verfasserin aut Maria Antonova verfasserin aut Pamela Schultz verfasserin aut Michael Angell verfasserin aut Daniel Clemans verfasserin aut Timothy Friebe verfasserin aut David Sherman verfasserin aut Anne M. Casper verfasserin aut Paul A. Price verfasserin aut Ashootosh Tripathi verfasserin aut In Marine Drugs MDPI AG, 2005 19(2021), 8, p 424 (DE-627)477992420 (DE-600)2175190-0 16603397 nnns volume:19 year:2021 number:8, p 424 https://doi.org/10.3390/md19080424 kostenfrei https://doaj.org/article/09f8b4e7cf2946e7962b7c8fafdf8b6b kostenfrei https://www.mdpi.com/1660-3397/19/8/424 kostenfrei https://doaj.org/toc/1660-3397 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2021 8, p 424
allfieldsSound	10.3390/md19080424 doi (DE-627)DOAJ085556351 (DE-599)DOAJ09f8b4e7cf2946e7962b7c8fafdf8b6b DE-627 ger DE-627 rakwb eng QH301-705.5 Osama G. Mohamed verfasserin aut Identification of a New Antimicrobial, Desertomycin H, Utilizing a Modified Crowded Plate Technique 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The antibiotic-resistant bacteria-associated infections are a major global healthcare threat. New classes of antimicrobial compounds are urgently needed as the frequency of infections caused by multidrug-resistant microbes continues to rise. Recent metagenomic data have demonstrated that there is still biosynthetic potential encoded in but transcriptionally silent in cultivatable bacterial genomes. However, the culture conditions required to identify and express silent biosynthetic gene clusters that yield natural products with antimicrobial activity are largely unknown. Here, we describe a new antibiotic discovery scheme, dubbed the modified crowded plate technique (mCPT), that utilizes complex microbial interactions to elicit antimicrobial production from otherwise silent biosynthetic gene clusters. Using the mCPT as part of the antibiotic crowdsourcing educational program Tiny Earth<sup<TM</sup<, we isolated over 1400 antibiotic-producing microbes, including 62 showing activity against multidrug-resistant pathogens. The natural product extracts generated from six microbial isolates showed potent activity against vancomycin-intermediate resistant <i<Staphylococcus aureus</i<. We utilized a targeted approach that coupled mass spectrometry data with bioactivity, yielding a new macrolactone class of metabolite, desertomycin H. In this study, we successfully demonstrate a concept that significantly increased our ability to quickly and efficiently identify microbes capable of the silent antibiotic production. antibiotic discovery crowded plate technique ESKAPE pathogens natural products Tiny Earth<sup<TM</sup< Biology (General) Sadaf Dorandish verfasserin aut Rebecca Lindow verfasserin aut Megan Steltz verfasserin aut Ifrah Shoukat verfasserin aut Maira Shoukat verfasserin aut Hussein Chehade verfasserin aut Sara Baghdadi verfasserin aut Madelaine McAlister-Raeburn verfasserin aut Asad Kamal verfasserin aut Dawit Abebe verfasserin aut Khaled Ali verfasserin aut Chelsey Ivy verfasserin aut Maria Antonova verfasserin aut Pamela Schultz verfasserin aut Michael Angell verfasserin aut Daniel Clemans verfasserin aut Timothy Friebe verfasserin aut David Sherman verfasserin aut Anne M. Casper verfasserin aut Paul A. Price verfasserin aut Ashootosh Tripathi verfasserin aut In Marine Drugs MDPI AG, 2005 19(2021), 8, p 424 (DE-627)477992420 (DE-600)2175190-0 16603397 nnns volume:19 year:2021 number:8, p 424 https://doi.org/10.3390/md19080424 kostenfrei https://doaj.org/article/09f8b4e7cf2946e7962b7c8fafdf8b6b kostenfrei https://www.mdpi.com/1660-3397/19/8/424 kostenfrei https://doaj.org/toc/1660-3397 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2021 8, p 424
language	English
source	In Marine Drugs 19(2021), 8, p 424 volume:19 year:2021 number:8, p 424
sourceStr	In Marine Drugs 19(2021), 8, p 424 volume:19 year:2021 number:8, p 424
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	antibiotic discovery crowded plate technique ESKAPE pathogens natural products Tiny Earth<sup<TM</sup< Biology (General)
isfreeaccess_bool	true
container_title	Marine Drugs
authorswithroles_txt_mv	Osama G. Mohamed @@aut@@ Sadaf Dorandish @@aut@@ Rebecca Lindow @@aut@@ Megan Steltz @@aut@@ Ifrah Shoukat @@aut@@ Maira Shoukat @@aut@@ Hussein Chehade @@aut@@ Sara Baghdadi @@aut@@ Madelaine McAlister-Raeburn @@aut@@ Asad Kamal @@aut@@ Dawit Abebe @@aut@@ Khaled Ali @@aut@@ Chelsey Ivy @@aut@@ Maria Antonova @@aut@@ Pamela Schultz @@aut@@ Michael Angell @@aut@@ Daniel Clemans @@aut@@ Timothy Friebe @@aut@@ David Sherman @@aut@@ Anne M. Casper @@aut@@ Paul A. Price @@aut@@ Ashootosh Tripathi @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	477992420
id	DOAJ085556351
language_de	englisch
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callnumber-first	Q - Science
author	Osama G. Mohamed
spellingShingle	Osama G. Mohamed misc QH301-705.5 misc antibiotic discovery misc crowded plate technique misc ESKAPE pathogens misc natural products misc Tiny Earth<sup<TM</sup< misc Biology (General) Identification of a New Antimicrobial, Desertomycin H, Utilizing a Modified Crowded Plate Technique
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topic_title	QH301-705.5 Identification of a New Antimicrobial, Desertomycin H, Utilizing a Modified Crowded Plate Technique antibiotic discovery crowded plate technique ESKAPE pathogens natural products Tiny Earth<sup<TM</sup<
topic	misc QH301-705.5 misc antibiotic discovery misc crowded plate technique misc ESKAPE pathogens misc natural products misc Tiny Earth<sup<TM</sup< misc Biology (General)
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title	Identification of a New Antimicrobial, Desertomycin H, Utilizing a Modified Crowded Plate Technique
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title_full	Identification of a New Antimicrobial, Desertomycin H, Utilizing a Modified Crowded Plate Technique
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author_browse	Osama G. Mohamed Sadaf Dorandish Rebecca Lindow Megan Steltz Ifrah Shoukat Maira Shoukat Hussein Chehade Sara Baghdadi Madelaine McAlister-Raeburn Asad Kamal Dawit Abebe Khaled Ali Chelsey Ivy Maria Antonova Pamela Schultz Michael Angell Daniel Clemans Timothy Friebe David Sherman Anne M. Casper Paul A. Price Ashootosh Tripathi
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title_sort	identification of a new antimicrobial, desertomycin h, utilizing a modified crowded plate technique
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title_auth	Identification of a New Antimicrobial, Desertomycin H, Utilizing a Modified Crowded Plate Technique
abstract	The antibiotic-resistant bacteria-associated infections are a major global healthcare threat. New classes of antimicrobial compounds are urgently needed as the frequency of infections caused by multidrug-resistant microbes continues to rise. Recent metagenomic data have demonstrated that there is still biosynthetic potential encoded in but transcriptionally silent in cultivatable bacterial genomes. However, the culture conditions required to identify and express silent biosynthetic gene clusters that yield natural products with antimicrobial activity are largely unknown. Here, we describe a new antibiotic discovery scheme, dubbed the modified crowded plate technique (mCPT), that utilizes complex microbial interactions to elicit antimicrobial production from otherwise silent biosynthetic gene clusters. Using the mCPT as part of the antibiotic crowdsourcing educational program Tiny Earth<sup<TM</sup<, we isolated over 1400 antibiotic-producing microbes, including 62 showing activity against multidrug-resistant pathogens. The natural product extracts generated from six microbial isolates showed potent activity against vancomycin-intermediate resistant <i<Staphylococcus aureus</i<. We utilized a targeted approach that coupled mass spectrometry data with bioactivity, yielding a new macrolactone class of metabolite, desertomycin H. In this study, we successfully demonstrate a concept that significantly increased our ability to quickly and efficiently identify microbes capable of the silent antibiotic production.
abstractGer	The antibiotic-resistant bacteria-associated infections are a major global healthcare threat. New classes of antimicrobial compounds are urgently needed as the frequency of infections caused by multidrug-resistant microbes continues to rise. Recent metagenomic data have demonstrated that there is still biosynthetic potential encoded in but transcriptionally silent in cultivatable bacterial genomes. However, the culture conditions required to identify and express silent biosynthetic gene clusters that yield natural products with antimicrobial activity are largely unknown. Here, we describe a new antibiotic discovery scheme, dubbed the modified crowded plate technique (mCPT), that utilizes complex microbial interactions to elicit antimicrobial production from otherwise silent biosynthetic gene clusters. Using the mCPT as part of the antibiotic crowdsourcing educational program Tiny Earth<sup<TM</sup<, we isolated over 1400 antibiotic-producing microbes, including 62 showing activity against multidrug-resistant pathogens. The natural product extracts generated from six microbial isolates showed potent activity against vancomycin-intermediate resistant <i<Staphylococcus aureus</i<. We utilized a targeted approach that coupled mass spectrometry data with bioactivity, yielding a new macrolactone class of metabolite, desertomycin H. In this study, we successfully demonstrate a concept that significantly increased our ability to quickly and efficiently identify microbes capable of the silent antibiotic production.
abstract_unstemmed	The antibiotic-resistant bacteria-associated infections are a major global healthcare threat. New classes of antimicrobial compounds are urgently needed as the frequency of infections caused by multidrug-resistant microbes continues to rise. Recent metagenomic data have demonstrated that there is still biosynthetic potential encoded in but transcriptionally silent in cultivatable bacterial genomes. However, the culture conditions required to identify and express silent biosynthetic gene clusters that yield natural products with antimicrobial activity are largely unknown. Here, we describe a new antibiotic discovery scheme, dubbed the modified crowded plate technique (mCPT), that utilizes complex microbial interactions to elicit antimicrobial production from otherwise silent biosynthetic gene clusters. Using the mCPT as part of the antibiotic crowdsourcing educational program Tiny Earth<sup<TM</sup<, we isolated over 1400 antibiotic-producing microbes, including 62 showing activity against multidrug-resistant pathogens. The natural product extracts generated from six microbial isolates showed potent activity against vancomycin-intermediate resistant <i<Staphylococcus aureus</i<. We utilized a targeted approach that coupled mass spectrometry data with bioactivity, yielding a new macrolactone class of metabolite, desertomycin H. In this study, we successfully demonstrate a concept that significantly increased our ability to quickly and efficiently identify microbes capable of the silent antibiotic production.
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title_short	Identification of a New Antimicrobial, Desertomycin H, Utilizing a Modified Crowded Plate Technique
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Identification of a New Antimicrobial, Desertomycin H, Utilizing a Modified Crowded Plate Technique

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