Comparative Transcriptome Analysis of <i<Bombyx mori</i< (Lepidoptera) Larval Hemolymph in Response to <i<Autographa californica</i< Nucleopolyhedrovirus in Differentially Resistant Strains

<i<Bombyx mori</i< nucleopolyhedrovirus (BmNPV) is a kind of pathogen that causes huge economic losses to silkworm production. Although <i<Autographa californica</i< nucleopolyhedrovirus (AcMNPV) and BmNPV are both baculoviruses, the host domains of these two viruses have alm...
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Autor*in:	Xin-yi Ding [verfasserIn] Xue-yang Wang [verfasserIn] Yun-hui Kong [verfasserIn] Chun-xiao Zhao [verfasserIn] Sheng Qin [verfasserIn] Xia Sun [verfasserIn] Mu-wang Li [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	AcMNPV transcriptome analysis apoptosis

Übergeordnetes Werk:	In: Processes - MDPI AG, 2013, 9(2021), 1401, p 1401
Übergeordnetes Werk:	volume:9 ; year:2021 ; number:1401, p 1401

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/pr9081401

Katalog-ID:	DOAJ085565598

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allfields	10.3390/pr9081401 doi (DE-627)DOAJ085565598 (DE-599)DOAJ12f9dbcbe5514eae8ecb16eb3a666b99 DE-627 ger DE-627 rakwb eng TP1-1185 QD1-999 Xin-yi Ding verfasserin aut Comparative Transcriptome Analysis of <i<Bombyx mori</i< (Lepidoptera) Larval Hemolymph in Response to <i<Autographa californica</i< Nucleopolyhedrovirus in Differentially Resistant Strains 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Bombyx mori</i< nucleopolyhedrovirus (BmNPV) is a kind of pathogen that causes huge economic losses to silkworm production. Although <i<Autographa californica</i< nucleopolyhedrovirus (AcMNPV) and BmNPV are both baculoviruses, the host domains of these two viruses have almost no intersection in nature. Recently, it has been found that some silkworms could be infected by recombinant AcMNPV through a puncture, which provided valuable material for studying the infection mechanism of baculovirus to silkworm. In this study, comparative transcriptomics was used to analyse the hemolymph of two differentially resistant strains following AcMNPV inoculation. There were 678 DEGs in p50 and 515 DEGs in C108 following viral infection. Among them, the upregulation and downregulation of DEGs were similar in p50; however, the upregulated DEGs were nearly twice as numerous as the downregulated DEGs in C108. The DEGs in different resistant strains differed by GO enrichment. Based on KEGG enrichment, DEGs were mainly enriched in metabolic pathways in p50 and the apoptosis pathway in C108. Moreover, 13 genes involved in metabolic pathways and 11 genes involved in the apoptosis pathway were analysed. Among the DEGs involved in apoptosis, the function of <i<BmTex261</i< in viral infection was analysed. The <i<BmTex261</i< showed the highest expression in hemolymph and a significant response to viral infection in the hemolymph of C108, indicating that it is involved in anti-AcMNPV infection. This was further validated by the significantly decreased expression of viral gene <i<lef3</i< after overexpression of <i<BmTex261</i< in BmN cells. The results provide a theoretical reference for the molecular mechanism of resistance to BmNPV in silkworms. <i<Bombyx mori</i< AcMNPV transcriptome analysis apoptosis <i<testis expressed genes 261</i< Chemical technology Chemistry Xue-yang Wang verfasserin aut Yun-hui Kong verfasserin aut Chun-xiao Zhao verfasserin aut Sheng Qin verfasserin aut Xia Sun verfasserin aut Mu-wang Li verfasserin aut In Processes MDPI AG, 2013 9(2021), 1401, p 1401 (DE-627)750371439 (DE-600)2720994-5 22279717 nnns volume:9 year:2021 number:1401, p 1401 https://doi.org/10.3390/pr9081401 kostenfrei https://doaj.org/article/12f9dbcbe5514eae8ecb16eb3a666b99 kostenfrei https://www.mdpi.com/2227-9717/9/8/1401 kostenfrei https://doaj.org/toc/2227-9717 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 1401, p 1401
spelling	10.3390/pr9081401 doi (DE-627)DOAJ085565598 (DE-599)DOAJ12f9dbcbe5514eae8ecb16eb3a666b99 DE-627 ger DE-627 rakwb eng TP1-1185 QD1-999 Xin-yi Ding verfasserin aut Comparative Transcriptome Analysis of <i<Bombyx mori</i< (Lepidoptera) Larval Hemolymph in Response to <i<Autographa californica</i< Nucleopolyhedrovirus in Differentially Resistant Strains 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Bombyx mori</i< nucleopolyhedrovirus (BmNPV) is a kind of pathogen that causes huge economic losses to silkworm production. Although <i<Autographa californica</i< nucleopolyhedrovirus (AcMNPV) and BmNPV are both baculoviruses, the host domains of these two viruses have almost no intersection in nature. Recently, it has been found that some silkworms could be infected by recombinant AcMNPV through a puncture, which provided valuable material for studying the infection mechanism of baculovirus to silkworm. In this study, comparative transcriptomics was used to analyse the hemolymph of two differentially resistant strains following AcMNPV inoculation. There were 678 DEGs in p50 and 515 DEGs in C108 following viral infection. Among them, the upregulation and downregulation of DEGs were similar in p50; however, the upregulated DEGs were nearly twice as numerous as the downregulated DEGs in C108. The DEGs in different resistant strains differed by GO enrichment. Based on KEGG enrichment, DEGs were mainly enriched in metabolic pathways in p50 and the apoptosis pathway in C108. Moreover, 13 genes involved in metabolic pathways and 11 genes involved in the apoptosis pathway were analysed. Among the DEGs involved in apoptosis, the function of <i<BmTex261</i< in viral infection was analysed. The <i<BmTex261</i< showed the highest expression in hemolymph and a significant response to viral infection in the hemolymph of C108, indicating that it is involved in anti-AcMNPV infection. This was further validated by the significantly decreased expression of viral gene <i<lef3</i< after overexpression of <i<BmTex261</i< in BmN cells. The results provide a theoretical reference for the molecular mechanism of resistance to BmNPV in silkworms. <i<Bombyx mori</i< AcMNPV transcriptome analysis apoptosis <i<testis expressed genes 261</i< Chemical technology Chemistry Xue-yang Wang verfasserin aut Yun-hui Kong verfasserin aut Chun-xiao Zhao verfasserin aut Sheng Qin verfasserin aut Xia Sun verfasserin aut Mu-wang Li verfasserin aut In Processes MDPI AG, 2013 9(2021), 1401, p 1401 (DE-627)750371439 (DE-600)2720994-5 22279717 nnns volume:9 year:2021 number:1401, p 1401 https://doi.org/10.3390/pr9081401 kostenfrei https://doaj.org/article/12f9dbcbe5514eae8ecb16eb3a666b99 kostenfrei https://www.mdpi.com/2227-9717/9/8/1401 kostenfrei https://doaj.org/toc/2227-9717 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 1401, p 1401
allfields_unstemmed	10.3390/pr9081401 doi (DE-627)DOAJ085565598 (DE-599)DOAJ12f9dbcbe5514eae8ecb16eb3a666b99 DE-627 ger DE-627 rakwb eng TP1-1185 QD1-999 Xin-yi Ding verfasserin aut Comparative Transcriptome Analysis of <i<Bombyx mori</i< (Lepidoptera) Larval Hemolymph in Response to <i<Autographa californica</i< Nucleopolyhedrovirus in Differentially Resistant Strains 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Bombyx mori</i< nucleopolyhedrovirus (BmNPV) is a kind of pathogen that causes huge economic losses to silkworm production. Although <i<Autographa californica</i< nucleopolyhedrovirus (AcMNPV) and BmNPV are both baculoviruses, the host domains of these two viruses have almost no intersection in nature. Recently, it has been found that some silkworms could be infected by recombinant AcMNPV through a puncture, which provided valuable material for studying the infection mechanism of baculovirus to silkworm. In this study, comparative transcriptomics was used to analyse the hemolymph of two differentially resistant strains following AcMNPV inoculation. There were 678 DEGs in p50 and 515 DEGs in C108 following viral infection. Among them, the upregulation and downregulation of DEGs were similar in p50; however, the upregulated DEGs were nearly twice as numerous as the downregulated DEGs in C108. The DEGs in different resistant strains differed by GO enrichment. Based on KEGG enrichment, DEGs were mainly enriched in metabolic pathways in p50 and the apoptosis pathway in C108. Moreover, 13 genes involved in metabolic pathways and 11 genes involved in the apoptosis pathway were analysed. Among the DEGs involved in apoptosis, the function of <i<BmTex261</i< in viral infection was analysed. The <i<BmTex261</i< showed the highest expression in hemolymph and a significant response to viral infection in the hemolymph of C108, indicating that it is involved in anti-AcMNPV infection. This was further validated by the significantly decreased expression of viral gene <i<lef3</i< after overexpression of <i<BmTex261</i< in BmN cells. The results provide a theoretical reference for the molecular mechanism of resistance to BmNPV in silkworms. <i<Bombyx mori</i< AcMNPV transcriptome analysis apoptosis <i<testis expressed genes 261</i< Chemical technology Chemistry Xue-yang Wang verfasserin aut Yun-hui Kong verfasserin aut Chun-xiao Zhao verfasserin aut Sheng Qin verfasserin aut Xia Sun verfasserin aut Mu-wang Li verfasserin aut In Processes MDPI AG, 2013 9(2021), 1401, p 1401 (DE-627)750371439 (DE-600)2720994-5 22279717 nnns volume:9 year:2021 number:1401, p 1401 https://doi.org/10.3390/pr9081401 kostenfrei https://doaj.org/article/12f9dbcbe5514eae8ecb16eb3a666b99 kostenfrei https://www.mdpi.com/2227-9717/9/8/1401 kostenfrei https://doaj.org/toc/2227-9717 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 1401, p 1401
allfieldsGer	10.3390/pr9081401 doi (DE-627)DOAJ085565598 (DE-599)DOAJ12f9dbcbe5514eae8ecb16eb3a666b99 DE-627 ger DE-627 rakwb eng TP1-1185 QD1-999 Xin-yi Ding verfasserin aut Comparative Transcriptome Analysis of <i<Bombyx mori</i< (Lepidoptera) Larval Hemolymph in Response to <i<Autographa californica</i< Nucleopolyhedrovirus in Differentially Resistant Strains 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Bombyx mori</i< nucleopolyhedrovirus (BmNPV) is a kind of pathogen that causes huge economic losses to silkworm production. Although <i<Autographa californica</i< nucleopolyhedrovirus (AcMNPV) and BmNPV are both baculoviruses, the host domains of these two viruses have almost no intersection in nature. Recently, it has been found that some silkworms could be infected by recombinant AcMNPV through a puncture, which provided valuable material for studying the infection mechanism of baculovirus to silkworm. In this study, comparative transcriptomics was used to analyse the hemolymph of two differentially resistant strains following AcMNPV inoculation. There were 678 DEGs in p50 and 515 DEGs in C108 following viral infection. Among them, the upregulation and downregulation of DEGs were similar in p50; however, the upregulated DEGs were nearly twice as numerous as the downregulated DEGs in C108. The DEGs in different resistant strains differed by GO enrichment. Based on KEGG enrichment, DEGs were mainly enriched in metabolic pathways in p50 and the apoptosis pathway in C108. Moreover, 13 genes involved in metabolic pathways and 11 genes involved in the apoptosis pathway were analysed. Among the DEGs involved in apoptosis, the function of <i<BmTex261</i< in viral infection was analysed. The <i<BmTex261</i< showed the highest expression in hemolymph and a significant response to viral infection in the hemolymph of C108, indicating that it is involved in anti-AcMNPV infection. This was further validated by the significantly decreased expression of viral gene <i<lef3</i< after overexpression of <i<BmTex261</i< in BmN cells. The results provide a theoretical reference for the molecular mechanism of resistance to BmNPV in silkworms. <i<Bombyx mori</i< AcMNPV transcriptome analysis apoptosis <i<testis expressed genes 261</i< Chemical technology Chemistry Xue-yang Wang verfasserin aut Yun-hui Kong verfasserin aut Chun-xiao Zhao verfasserin aut Sheng Qin verfasserin aut Xia Sun verfasserin aut Mu-wang Li verfasserin aut In Processes MDPI AG, 2013 9(2021), 1401, p 1401 (DE-627)750371439 (DE-600)2720994-5 22279717 nnns volume:9 year:2021 number:1401, p 1401 https://doi.org/10.3390/pr9081401 kostenfrei https://doaj.org/article/12f9dbcbe5514eae8ecb16eb3a666b99 kostenfrei https://www.mdpi.com/2227-9717/9/8/1401 kostenfrei https://doaj.org/toc/2227-9717 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 1401, p 1401
allfieldsSound	10.3390/pr9081401 doi (DE-627)DOAJ085565598 (DE-599)DOAJ12f9dbcbe5514eae8ecb16eb3a666b99 DE-627 ger DE-627 rakwb eng TP1-1185 QD1-999 Xin-yi Ding verfasserin aut Comparative Transcriptome Analysis of <i<Bombyx mori</i< (Lepidoptera) Larval Hemolymph in Response to <i<Autographa californica</i< Nucleopolyhedrovirus in Differentially Resistant Strains 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Bombyx mori</i< nucleopolyhedrovirus (BmNPV) is a kind of pathogen that causes huge economic losses to silkworm production. Although <i<Autographa californica</i< nucleopolyhedrovirus (AcMNPV) and BmNPV are both baculoviruses, the host domains of these two viruses have almost no intersection in nature. Recently, it has been found that some silkworms could be infected by recombinant AcMNPV through a puncture, which provided valuable material for studying the infection mechanism of baculovirus to silkworm. In this study, comparative transcriptomics was used to analyse the hemolymph of two differentially resistant strains following AcMNPV inoculation. There were 678 DEGs in p50 and 515 DEGs in C108 following viral infection. Among them, the upregulation and downregulation of DEGs were similar in p50; however, the upregulated DEGs were nearly twice as numerous as the downregulated DEGs in C108. The DEGs in different resistant strains differed by GO enrichment. Based on KEGG enrichment, DEGs were mainly enriched in metabolic pathways in p50 and the apoptosis pathway in C108. Moreover, 13 genes involved in metabolic pathways and 11 genes involved in the apoptosis pathway were analysed. Among the DEGs involved in apoptosis, the function of <i<BmTex261</i< in viral infection was analysed. The <i<BmTex261</i< showed the highest expression in hemolymph and a significant response to viral infection in the hemolymph of C108, indicating that it is involved in anti-AcMNPV infection. This was further validated by the significantly decreased expression of viral gene <i<lef3</i< after overexpression of <i<BmTex261</i< in BmN cells. The results provide a theoretical reference for the molecular mechanism of resistance to BmNPV in silkworms. <i<Bombyx mori</i< AcMNPV transcriptome analysis apoptosis <i<testis expressed genes 261</i< Chemical technology Chemistry Xue-yang Wang verfasserin aut Yun-hui Kong verfasserin aut Chun-xiao Zhao verfasserin aut Sheng Qin verfasserin aut Xia Sun verfasserin aut Mu-wang Li verfasserin aut In Processes MDPI AG, 2013 9(2021), 1401, p 1401 (DE-627)750371439 (DE-600)2720994-5 22279717 nnns volume:9 year:2021 number:1401, p 1401 https://doi.org/10.3390/pr9081401 kostenfrei https://doaj.org/article/12f9dbcbe5514eae8ecb16eb3a666b99 kostenfrei https://www.mdpi.com/2227-9717/9/8/1401 kostenfrei https://doaj.org/toc/2227-9717 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 1401, p 1401
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author	Xin-yi Ding
spellingShingle	Xin-yi Ding misc TP1-1185 misc QD1-999 misc <i<Bombyx mori</i< misc AcMNPV misc transcriptome analysis misc apoptosis misc <i<testis expressed genes 261</i< misc Chemical technology misc Chemistry Comparative Transcriptome Analysis of <i<Bombyx mori</i< (Lepidoptera) Larval Hemolymph in Response to <i<Autographa californica</i< Nucleopolyhedrovirus in Differentially Resistant Strains
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topic_title	TP1-1185 QD1-999 Comparative Transcriptome Analysis of <i<Bombyx mori</i< (Lepidoptera) Larval Hemolymph in Response to <i<Autographa californica</i< Nucleopolyhedrovirus in Differentially Resistant Strains <i<Bombyx mori</i< AcMNPV transcriptome analysis apoptosis <i<testis expressed genes 261</i<
topic	misc TP1-1185 misc QD1-999 misc <i<Bombyx mori</i< misc AcMNPV misc transcriptome analysis misc apoptosis misc <i<testis expressed genes 261</i< misc Chemical technology misc Chemistry
topic_unstemmed	misc TP1-1185 misc QD1-999 misc <i<Bombyx mori</i< misc AcMNPV misc transcriptome analysis misc apoptosis misc <i<testis expressed genes 261</i< misc Chemical technology misc Chemistry
topic_browse	misc TP1-1185 misc QD1-999 misc <i<Bombyx mori</i< misc AcMNPV misc transcriptome analysis misc apoptosis misc <i<testis expressed genes 261</i< misc Chemical technology misc Chemistry
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title	Comparative Transcriptome Analysis of <i<Bombyx mori</i< (Lepidoptera) Larval Hemolymph in Response to <i<Autographa californica</i< Nucleopolyhedrovirus in Differentially Resistant Strains
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title_full	Comparative Transcriptome Analysis of <i<Bombyx mori</i< (Lepidoptera) Larval Hemolymph in Response to <i<Autographa californica</i< Nucleopolyhedrovirus in Differentially Resistant Strains
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author_browse	Xin-yi Ding Xue-yang Wang Yun-hui Kong Chun-xiao Zhao Sheng Qin Xia Sun Mu-wang Li
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class	TP1-1185 QD1-999
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author-letter	Xin-yi Ding
doi_str_mv	10.3390/pr9081401
author2-role	verfasserin
title_sort	comparative transcriptome analysis of <i<bombyx mori</i< (lepidoptera) larval hemolymph in response to <i<autographa californica</i< nucleopolyhedrovirus in differentially resistant strains
callnumber	TP1-1185
title_auth	Comparative Transcriptome Analysis of <i<Bombyx mori</i< (Lepidoptera) Larval Hemolymph in Response to <i<Autographa californica</i< Nucleopolyhedrovirus in Differentially Resistant Strains
abstract	<i<Bombyx mori</i< nucleopolyhedrovirus (BmNPV) is a kind of pathogen that causes huge economic losses to silkworm production. Although <i<Autographa californica</i< nucleopolyhedrovirus (AcMNPV) and BmNPV are both baculoviruses, the host domains of these two viruses have almost no intersection in nature. Recently, it has been found that some silkworms could be infected by recombinant AcMNPV through a puncture, which provided valuable material for studying the infection mechanism of baculovirus to silkworm. In this study, comparative transcriptomics was used to analyse the hemolymph of two differentially resistant strains following AcMNPV inoculation. There were 678 DEGs in p50 and 515 DEGs in C108 following viral infection. Among them, the upregulation and downregulation of DEGs were similar in p50; however, the upregulated DEGs were nearly twice as numerous as the downregulated DEGs in C108. The DEGs in different resistant strains differed by GO enrichment. Based on KEGG enrichment, DEGs were mainly enriched in metabolic pathways in p50 and the apoptosis pathway in C108. Moreover, 13 genes involved in metabolic pathways and 11 genes involved in the apoptosis pathway were analysed. Among the DEGs involved in apoptosis, the function of <i<BmTex261</i< in viral infection was analysed. The <i<BmTex261</i< showed the highest expression in hemolymph and a significant response to viral infection in the hemolymph of C108, indicating that it is involved in anti-AcMNPV infection. This was further validated by the significantly decreased expression of viral gene <i<lef3</i< after overexpression of <i<BmTex261</i< in BmN cells. The results provide a theoretical reference for the molecular mechanism of resistance to BmNPV in silkworms.
abstractGer	<i<Bombyx mori</i< nucleopolyhedrovirus (BmNPV) is a kind of pathogen that causes huge economic losses to silkworm production. Although <i<Autographa californica</i< nucleopolyhedrovirus (AcMNPV) and BmNPV are both baculoviruses, the host domains of these two viruses have almost no intersection in nature. Recently, it has been found that some silkworms could be infected by recombinant AcMNPV through a puncture, which provided valuable material for studying the infection mechanism of baculovirus to silkworm. In this study, comparative transcriptomics was used to analyse the hemolymph of two differentially resistant strains following AcMNPV inoculation. There were 678 DEGs in p50 and 515 DEGs in C108 following viral infection. Among them, the upregulation and downregulation of DEGs were similar in p50; however, the upregulated DEGs were nearly twice as numerous as the downregulated DEGs in C108. The DEGs in different resistant strains differed by GO enrichment. Based on KEGG enrichment, DEGs were mainly enriched in metabolic pathways in p50 and the apoptosis pathway in C108. Moreover, 13 genes involved in metabolic pathways and 11 genes involved in the apoptosis pathway were analysed. Among the DEGs involved in apoptosis, the function of <i<BmTex261</i< in viral infection was analysed. The <i<BmTex261</i< showed the highest expression in hemolymph and a significant response to viral infection in the hemolymph of C108, indicating that it is involved in anti-AcMNPV infection. This was further validated by the significantly decreased expression of viral gene <i<lef3</i< after overexpression of <i<BmTex261</i< in BmN cells. The results provide a theoretical reference for the molecular mechanism of resistance to BmNPV in silkworms.
abstract_unstemmed	<i<Bombyx mori</i< nucleopolyhedrovirus (BmNPV) is a kind of pathogen that causes huge economic losses to silkworm production. Although <i<Autographa californica</i< nucleopolyhedrovirus (AcMNPV) and BmNPV are both baculoviruses, the host domains of these two viruses have almost no intersection in nature. Recently, it has been found that some silkworms could be infected by recombinant AcMNPV through a puncture, which provided valuable material for studying the infection mechanism of baculovirus to silkworm. In this study, comparative transcriptomics was used to analyse the hemolymph of two differentially resistant strains following AcMNPV inoculation. There were 678 DEGs in p50 and 515 DEGs in C108 following viral infection. Among them, the upregulation and downregulation of DEGs were similar in p50; however, the upregulated DEGs were nearly twice as numerous as the downregulated DEGs in C108. The DEGs in different resistant strains differed by GO enrichment. Based on KEGG enrichment, DEGs were mainly enriched in metabolic pathways in p50 and the apoptosis pathway in C108. Moreover, 13 genes involved in metabolic pathways and 11 genes involved in the apoptosis pathway were analysed. Among the DEGs involved in apoptosis, the function of <i<BmTex261</i< in viral infection was analysed. The <i<BmTex261</i< showed the highest expression in hemolymph and a significant response to viral infection in the hemolymph of C108, indicating that it is involved in anti-AcMNPV infection. This was further validated by the significantly decreased expression of viral gene <i<lef3</i< after overexpression of <i<BmTex261</i< in BmN cells. The results provide a theoretical reference for the molecular mechanism of resistance to BmNPV in silkworms.
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title_short	Comparative Transcriptome Analysis of <i<Bombyx mori</i< (Lepidoptera) Larval Hemolymph in Response to <i<Autographa californica</i< Nucleopolyhedrovirus in Differentially Resistant Strains
url	https://doi.org/10.3390/pr9081401 https://doaj.org/article/12f9dbcbe5514eae8ecb16eb3a666b99 https://www.mdpi.com/2227-9717/9/8/1401 https://doaj.org/toc/2227-9717
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