Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological Samples

Gradient images can be obtained using a rotating square mask to filter the angular spectra of the wavefront generated by a complex transmittance object. This method can be applied to measure the three-dimensional structure of microscopic biological samples through the relationship of the phase with...
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Gespeichert in:

Autor*in:	Ignacio Iglesias [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	microscopy quantitative microscopy wavefront sensing

Übergeordnetes Werk:	In: Sensors - MDPI AG, 2003, 22(2022), 5, p 1842
Übergeordnetes Werk:	volume:22 ; year:2022 ; number:5, p 1842

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DOI / URN:	10.3390/s22051842

Katalog-ID:	DOAJ085584509

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callnumber-first	T - Technology
author	Ignacio Iglesias
spellingShingle	Ignacio Iglesias misc TP1-1185 misc microscopy misc quantitative microscopy misc wavefront sensing misc Chemical technology Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological Samples
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topic_title	TP1-1185 Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological Samples microscopy quantitative microscopy wavefront sensing
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title	Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological Samples
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title_full	Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological Samples
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title_sort	use of a rotating square spatial-frequency filter to map the optical path length variation in microscopic biological samples
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title_auth	Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological Samples
abstract	Gradient images can be obtained using a rotating square mask to filter the angular spectra of the wavefront generated by a complex transmittance object. This method can be applied to measure the three-dimensional structure of microscopic biological samples through the relationship of the phase with the optical path length. This work describes the implementation of a system using an inverted optical microscope and shows the experimental results of phase maps generated by boar sperm cells.
abstractGer	Gradient images can be obtained using a rotating square mask to filter the angular spectra of the wavefront generated by a complex transmittance object. This method can be applied to measure the three-dimensional structure of microscopic biological samples through the relationship of the phase with the optical path length. This work describes the implementation of a system using an inverted optical microscope and shows the experimental results of phase maps generated by boar sperm cells.
abstract_unstemmed	Gradient images can be obtained using a rotating square mask to filter the angular spectra of the wavefront generated by a complex transmittance object. This method can be applied to measure the three-dimensional structure of microscopic biological samples through the relationship of the phase with the optical path length. This work describes the implementation of a system using an inverted optical microscope and shows the experimental results of phase maps generated by boar sperm cells.
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title_short	Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological Samples
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