Enhancing the Expression of the <i<OsF3H</i< Gene in <i<Oryza sativa</i< Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance

The white-backed planthopper (WBPH) is a major pest of rice crops and causes severe loss of yield. We previously developed the WBPH-resistant rice cultivar “<i<OxF3H</i<” by overexpressing the <i<OsF3H</i< gene. Although there was a higher accumulation of the flavonoids kaempferol (Kr) and quercetin (Qu) as well as salicylic acid (SA) in <i<OxF3H</i< transgenic (<i<OsF3H</i< or Trans) plants compared to the wild type (WT), it is still unclear how Os<i<F3H</i< overexpression affects these WBPH resistant-related changes in gene expression in <i<OxF3H</i< plants. In this study, we analyze RNA-seq data from <i<OxF3H</i< and WT at several points (0 h, 3 h, 12 h, and 24 h) after WBPH infection to explain how overall changes in gene expression happen in these two cultivars. RT-qPCR further validated a number of the genes. Results revealed that the highest number of DEGs (4735) between the two genotypes was detected after 24 h of infection. Interestingly, it was found that several of the DEGs between the WT and <i<OsF3H</i< under control conditions were also differentially expressed in <i<OsF3H</i< in response to WBPH infestation. These results indicate that significant differences in gene expression between the “<i<OxF3H</i<” and “WT” exist as the infection time increases. Many of these DEGs were related to oxidoreductase activity, response to stress, salicylic acid biosynthesis, metabolic process, defense response to pathogen, cellular response to toxic substance, and regulation of hormone levels. Moreover, genes involved in salicylic acid (SA) and ethylene (Et) biosynthesis were upregulated in <i<OxF3H</i< plants, while jasmonic acid (JA), brassinosteroid (Br), and abscisic acid (ABA) signaling pathways were found downregulated in <i<OxF3H</i< plants during WBPH infestation. Interestingly, many DEGs related to pathogenesis, such as <i<OsPR1</i<, <i<OsPR1b</i<, <i<OsNPR1</i<, <i<OsNPR3,</i< and <i<OsNPR5,</i< were found to be significantly upregulated in <i<OxF3H</i< plants. Additionally, genes related to the MAPKs pathway and about 30 <i<WRKY</i< genes involved in different pathways were upregulated in <i<OxF3H</i< plants after WBPH infestation. This suggests that overexpression of the <i<OxF3H</i< gene leads to multiple transcriptomic changes and impacts plant hormones and pathogenic-related and secondary-metabolites-related genes, enhancing the plant’s resistance to WBPH infestation. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Rahmatullah Jan [verfasserIn] Sajjad Asaf [verfasserIn] Lubna [verfasserIn] Saleem Asif [verfasserIn] Eun-Gyeong Kim [verfasserIn] Yoon-Hee Jang [verfasserIn] Nari Kim [verfasserIn] Ahmed Al-Harrasi [verfasserIn] Gang-Seob Lee [verfasserIn] Kyung-Min Kim [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	pathogen defense system salicylic acid jasmonic acid transcriptomics

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 23(2022), 23, p 15308
Übergeordnetes Werk:	volume:23 ; year:2022 ; number:23, p 15308

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms232315308

Katalog-ID:	DOAJ085619191

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245	1	0	\|a Enhancing the Expression of the <i<OsF3H</i< Gene in <i<Oryza sativa</i< Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance
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520			\|a The white-backed planthopper (WBPH) is a major pest of rice crops and causes severe loss of yield. We previously developed the WBPH-resistant rice cultivar “<i<OxF3H</i<” by overexpressing the <i<OsF3H</i< gene. Although there was a higher accumulation of the flavonoids kaempferol (Kr) and quercetin (Qu) as well as salicylic acid (SA) in <i<OxF3H</i< transgenic (<i<OsF3H</i< or Trans) plants compared to the wild type (WT), it is still unclear how Os<i<F3H</i< overexpression affects these WBPH resistant-related changes in gene expression in <i<OxF3H</i< plants. In this study, we analyze RNA-seq data from <i<OxF3H</i< and WT at several points (0 h, 3 h, 12 h, and 24 h) after WBPH infection to explain how overall changes in gene expression happen in these two cultivars. RT-qPCR further validated a number of the genes. Results revealed that the highest number of DEGs (4735) between the two genotypes was detected after 24 h of infection. Interestingly, it was found that several of the DEGs between the WT and <i<OsF3H</i< under control conditions were also differentially expressed in <i<OsF3H</i< in response to WBPH infestation. These results indicate that significant differences in gene expression between the “<i<OxF3H</i<” and “WT” exist as the infection time increases. Many of these DEGs were related to oxidoreductase activity, response to stress, salicylic acid biosynthesis, metabolic process, defense response to pathogen, cellular response to toxic substance, and regulation of hormone levels. Moreover, genes involved in salicylic acid (SA) and ethylene (Et) biosynthesis were upregulated in <i<OxF3H</i< plants, while jasmonic acid (JA), brassinosteroid (Br), and abscisic acid (ABA) signaling pathways were found downregulated in <i<OxF3H</i< plants during WBPH infestation. Interestingly, many DEGs related to pathogenesis, such as <i<OsPR1</i<, <i<OsPR1b</i<, <i<OsNPR1</i<, <i<OsNPR3,</i< and <i<OsNPR5,</i< were found to be significantly upregulated in <i<OxF3H</i< plants. Additionally, genes related to the MAPKs pathway and about 30 <i<WRKY</i< genes involved in different pathways were upregulated in <i<OxF3H</i< plants after WBPH infestation. This suggests that overexpression of the <i<OxF3H</i< gene leads to multiple transcriptomic changes and impacts plant hormones and pathogenic-related and secondary-metabolites-related genes, enhancing the plant’s resistance to WBPH infestation.
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allfields	10.3390/ijms232315308 doi (DE-627)DOAJ085619191 (DE-599)DOAJ2dedc68170fa40e0bfff23c8f7c8272d DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Rahmatullah Jan verfasserin aut Enhancing the Expression of the <i<OsF3H</i< Gene in <i<Oryza sativa</i< Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The white-backed planthopper (WBPH) is a major pest of rice crops and causes severe loss of yield. We previously developed the WBPH-resistant rice cultivar “<i<OxF3H</i<” by overexpressing the <i<OsF3H</i< gene. Although there was a higher accumulation of the flavonoids kaempferol (Kr) and quercetin (Qu) as well as salicylic acid (SA) in <i<OxF3H</i< transgenic (<i<OsF3H</i< or Trans) plants compared to the wild type (WT), it is still unclear how Os<i<F3H</i< overexpression affects these WBPH resistant-related changes in gene expression in <i<OxF3H</i< plants. In this study, we analyze RNA-seq data from <i<OxF3H</i< and WT at several points (0 h, 3 h, 12 h, and 24 h) after WBPH infection to explain how overall changes in gene expression happen in these two cultivars. RT-qPCR further validated a number of the genes. Results revealed that the highest number of DEGs (4735) between the two genotypes was detected after 24 h of infection. Interestingly, it was found that several of the DEGs between the WT and <i<OsF3H</i< under control conditions were also differentially expressed in <i<OsF3H</i< in response to WBPH infestation. These results indicate that significant differences in gene expression between the “<i<OxF3H</i<” and “WT” exist as the infection time increases. Many of these DEGs were related to oxidoreductase activity, response to stress, salicylic acid biosynthesis, metabolic process, defense response to pathogen, cellular response to toxic substance, and regulation of hormone levels. Moreover, genes involved in salicylic acid (SA) and ethylene (Et) biosynthesis were upregulated in <i<OxF3H</i< plants, while jasmonic acid (JA), brassinosteroid (Br), and abscisic acid (ABA) signaling pathways were found downregulated in <i<OxF3H</i< plants during WBPH infestation. Interestingly, many DEGs related to pathogenesis, such as <i<OsPR1</i<, <i<OsPR1b</i<, <i<OsNPR1</i<, <i<OsNPR3,</i< and <i<OsNPR5,</i< were found to be significantly upregulated in <i<OxF3H</i< plants. Additionally, genes related to the MAPKs pathway and about 30 <i<WRKY</i< genes involved in different pathways were upregulated in <i<OxF3H</i< plants after WBPH infestation. This suggests that overexpression of the <i<OxF3H</i< gene leads to multiple transcriptomic changes and impacts plant hormones and pathogenic-related and secondary-metabolites-related genes, enhancing the plant’s resistance to WBPH infestation. <i<Oryza sativa</i< pathogen defense system salicylic acid jasmonic acid transcriptomics Biology (General) Chemistry Sajjad Asaf verfasserin aut Lubna verfasserin aut Saleem Asif verfasserin aut Eun-Gyeong Kim verfasserin aut Yoon-Hee Jang verfasserin aut Nari Kim verfasserin aut Ahmed Al-Harrasi verfasserin aut Gang-Seob Lee verfasserin aut Kyung-Min Kim verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 23, p 15308 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:23, p 15308 https://doi.org/10.3390/ijms232315308 kostenfrei https://doaj.org/article/2dedc68170fa40e0bfff23c8f7c8272d kostenfrei https://www.mdpi.com/1422-0067/23/23/15308 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 23, p 15308
spelling	10.3390/ijms232315308 doi (DE-627)DOAJ085619191 (DE-599)DOAJ2dedc68170fa40e0bfff23c8f7c8272d DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Rahmatullah Jan verfasserin aut Enhancing the Expression of the <i<OsF3H</i< Gene in <i<Oryza sativa</i< Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The white-backed planthopper (WBPH) is a major pest of rice crops and causes severe loss of yield. We previously developed the WBPH-resistant rice cultivar “<i<OxF3H</i<” by overexpressing the <i<OsF3H</i< gene. Although there was a higher accumulation of the flavonoids kaempferol (Kr) and quercetin (Qu) as well as salicylic acid (SA) in <i<OxF3H</i< transgenic (<i<OsF3H</i< or Trans) plants compared to the wild type (WT), it is still unclear how Os<i<F3H</i< overexpression affects these WBPH resistant-related changes in gene expression in <i<OxF3H</i< plants. In this study, we analyze RNA-seq data from <i<OxF3H</i< and WT at several points (0 h, 3 h, 12 h, and 24 h) after WBPH infection to explain how overall changes in gene expression happen in these two cultivars. RT-qPCR further validated a number of the genes. Results revealed that the highest number of DEGs (4735) between the two genotypes was detected after 24 h of infection. Interestingly, it was found that several of the DEGs between the WT and <i<OsF3H</i< under control conditions were also differentially expressed in <i<OsF3H</i< in response to WBPH infestation. These results indicate that significant differences in gene expression between the “<i<OxF3H</i<” and “WT” exist as the infection time increases. Many of these DEGs were related to oxidoreductase activity, response to stress, salicylic acid biosynthesis, metabolic process, defense response to pathogen, cellular response to toxic substance, and regulation of hormone levels. Moreover, genes involved in salicylic acid (SA) and ethylene (Et) biosynthesis were upregulated in <i<OxF3H</i< plants, while jasmonic acid (JA), brassinosteroid (Br), and abscisic acid (ABA) signaling pathways were found downregulated in <i<OxF3H</i< plants during WBPH infestation. Interestingly, many DEGs related to pathogenesis, such as <i<OsPR1</i<, <i<OsPR1b</i<, <i<OsNPR1</i<, <i<OsNPR3,</i< and <i<OsNPR5,</i< were found to be significantly upregulated in <i<OxF3H</i< plants. Additionally, genes related to the MAPKs pathway and about 30 <i<WRKY</i< genes involved in different pathways were upregulated in <i<OxF3H</i< plants after WBPH infestation. This suggests that overexpression of the <i<OxF3H</i< gene leads to multiple transcriptomic changes and impacts plant hormones and pathogenic-related and secondary-metabolites-related genes, enhancing the plant’s resistance to WBPH infestation. <i<Oryza sativa</i< pathogen defense system salicylic acid jasmonic acid transcriptomics Biology (General) Chemistry Sajjad Asaf verfasserin aut Lubna verfasserin aut Saleem Asif verfasserin aut Eun-Gyeong Kim verfasserin aut Yoon-Hee Jang verfasserin aut Nari Kim verfasserin aut Ahmed Al-Harrasi verfasserin aut Gang-Seob Lee verfasserin aut Kyung-Min Kim verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 23, p 15308 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:23, p 15308 https://doi.org/10.3390/ijms232315308 kostenfrei https://doaj.org/article/2dedc68170fa40e0bfff23c8f7c8272d kostenfrei https://www.mdpi.com/1422-0067/23/23/15308 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 23, p 15308
allfields_unstemmed	10.3390/ijms232315308 doi (DE-627)DOAJ085619191 (DE-599)DOAJ2dedc68170fa40e0bfff23c8f7c8272d DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Rahmatullah Jan verfasserin aut Enhancing the Expression of the <i<OsF3H</i< Gene in <i<Oryza sativa</i< Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The white-backed planthopper (WBPH) is a major pest of rice crops and causes severe loss of yield. We previously developed the WBPH-resistant rice cultivar “<i<OxF3H</i<” by overexpressing the <i<OsF3H</i< gene. Although there was a higher accumulation of the flavonoids kaempferol (Kr) and quercetin (Qu) as well as salicylic acid (SA) in <i<OxF3H</i< transgenic (<i<OsF3H</i< or Trans) plants compared to the wild type (WT), it is still unclear how Os<i<F3H</i< overexpression affects these WBPH resistant-related changes in gene expression in <i<OxF3H</i< plants. In this study, we analyze RNA-seq data from <i<OxF3H</i< and WT at several points (0 h, 3 h, 12 h, and 24 h) after WBPH infection to explain how overall changes in gene expression happen in these two cultivars. RT-qPCR further validated a number of the genes. Results revealed that the highest number of DEGs (4735) between the two genotypes was detected after 24 h of infection. Interestingly, it was found that several of the DEGs between the WT and <i<OsF3H</i< under control conditions were also differentially expressed in <i<OsF3H</i< in response to WBPH infestation. These results indicate that significant differences in gene expression between the “<i<OxF3H</i<” and “WT” exist as the infection time increases. Many of these DEGs were related to oxidoreductase activity, response to stress, salicylic acid biosynthesis, metabolic process, defense response to pathogen, cellular response to toxic substance, and regulation of hormone levels. Moreover, genes involved in salicylic acid (SA) and ethylene (Et) biosynthesis were upregulated in <i<OxF3H</i< plants, while jasmonic acid (JA), brassinosteroid (Br), and abscisic acid (ABA) signaling pathways were found downregulated in <i<OxF3H</i< plants during WBPH infestation. Interestingly, many DEGs related to pathogenesis, such as <i<OsPR1</i<, <i<OsPR1b</i<, <i<OsNPR1</i<, <i<OsNPR3,</i< and <i<OsNPR5,</i< were found to be significantly upregulated in <i<OxF3H</i< plants. Additionally, genes related to the MAPKs pathway and about 30 <i<WRKY</i< genes involved in different pathways were upregulated in <i<OxF3H</i< plants after WBPH infestation. This suggests that overexpression of the <i<OxF3H</i< gene leads to multiple transcriptomic changes and impacts plant hormones and pathogenic-related and secondary-metabolites-related genes, enhancing the plant’s resistance to WBPH infestation. <i<Oryza sativa</i< pathogen defense system salicylic acid jasmonic acid transcriptomics Biology (General) Chemistry Sajjad Asaf verfasserin aut Lubna verfasserin aut Saleem Asif verfasserin aut Eun-Gyeong Kim verfasserin aut Yoon-Hee Jang verfasserin aut Nari Kim verfasserin aut Ahmed Al-Harrasi verfasserin aut Gang-Seob Lee verfasserin aut Kyung-Min Kim verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 23, p 15308 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:23, p 15308 https://doi.org/10.3390/ijms232315308 kostenfrei https://doaj.org/article/2dedc68170fa40e0bfff23c8f7c8272d kostenfrei https://www.mdpi.com/1422-0067/23/23/15308 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 23, p 15308
allfieldsGer	10.3390/ijms232315308 doi (DE-627)DOAJ085619191 (DE-599)DOAJ2dedc68170fa40e0bfff23c8f7c8272d DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Rahmatullah Jan verfasserin aut Enhancing the Expression of the <i<OsF3H</i< Gene in <i<Oryza sativa</i< Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The white-backed planthopper (WBPH) is a major pest of rice crops and causes severe loss of yield. We previously developed the WBPH-resistant rice cultivar “<i<OxF3H</i<” by overexpressing the <i<OsF3H</i< gene. Although there was a higher accumulation of the flavonoids kaempferol (Kr) and quercetin (Qu) as well as salicylic acid (SA) in <i<OxF3H</i< transgenic (<i<OsF3H</i< or Trans) plants compared to the wild type (WT), it is still unclear how Os<i<F3H</i< overexpression affects these WBPH resistant-related changes in gene expression in <i<OxF3H</i< plants. In this study, we analyze RNA-seq data from <i<OxF3H</i< and WT at several points (0 h, 3 h, 12 h, and 24 h) after WBPH infection to explain how overall changes in gene expression happen in these two cultivars. RT-qPCR further validated a number of the genes. Results revealed that the highest number of DEGs (4735) between the two genotypes was detected after 24 h of infection. Interestingly, it was found that several of the DEGs between the WT and <i<OsF3H</i< under control conditions were also differentially expressed in <i<OsF3H</i< in response to WBPH infestation. These results indicate that significant differences in gene expression between the “<i<OxF3H</i<” and “WT” exist as the infection time increases. Many of these DEGs were related to oxidoreductase activity, response to stress, salicylic acid biosynthesis, metabolic process, defense response to pathogen, cellular response to toxic substance, and regulation of hormone levels. Moreover, genes involved in salicylic acid (SA) and ethylene (Et) biosynthesis were upregulated in <i<OxF3H</i< plants, while jasmonic acid (JA), brassinosteroid (Br), and abscisic acid (ABA) signaling pathways were found downregulated in <i<OxF3H</i< plants during WBPH infestation. Interestingly, many DEGs related to pathogenesis, such as <i<OsPR1</i<, <i<OsPR1b</i<, <i<OsNPR1</i<, <i<OsNPR3,</i< and <i<OsNPR5,</i< were found to be significantly upregulated in <i<OxF3H</i< plants. Additionally, genes related to the MAPKs pathway and about 30 <i<WRKY</i< genes involved in different pathways were upregulated in <i<OxF3H</i< plants after WBPH infestation. This suggests that overexpression of the <i<OxF3H</i< gene leads to multiple transcriptomic changes and impacts plant hormones and pathogenic-related and secondary-metabolites-related genes, enhancing the plant’s resistance to WBPH infestation. <i<Oryza sativa</i< pathogen defense system salicylic acid jasmonic acid transcriptomics Biology (General) Chemistry Sajjad Asaf verfasserin aut Lubna verfasserin aut Saleem Asif verfasserin aut Eun-Gyeong Kim verfasserin aut Yoon-Hee Jang verfasserin aut Nari Kim verfasserin aut Ahmed Al-Harrasi verfasserin aut Gang-Seob Lee verfasserin aut Kyung-Min Kim verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 23, p 15308 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:23, p 15308 https://doi.org/10.3390/ijms232315308 kostenfrei https://doaj.org/article/2dedc68170fa40e0bfff23c8f7c8272d kostenfrei https://www.mdpi.com/1422-0067/23/23/15308 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 23, p 15308
allfieldsSound	10.3390/ijms232315308 doi (DE-627)DOAJ085619191 (DE-599)DOAJ2dedc68170fa40e0bfff23c8f7c8272d DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Rahmatullah Jan verfasserin aut Enhancing the Expression of the <i<OsF3H</i< Gene in <i<Oryza sativa</i< Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The white-backed planthopper (WBPH) is a major pest of rice crops and causes severe loss of yield. We previously developed the WBPH-resistant rice cultivar “<i<OxF3H</i<” by overexpressing the <i<OsF3H</i< gene. Although there was a higher accumulation of the flavonoids kaempferol (Kr) and quercetin (Qu) as well as salicylic acid (SA) in <i<OxF3H</i< transgenic (<i<OsF3H</i< or Trans) plants compared to the wild type (WT), it is still unclear how Os<i<F3H</i< overexpression affects these WBPH resistant-related changes in gene expression in <i<OxF3H</i< plants. In this study, we analyze RNA-seq data from <i<OxF3H</i< and WT at several points (0 h, 3 h, 12 h, and 24 h) after WBPH infection to explain how overall changes in gene expression happen in these two cultivars. RT-qPCR further validated a number of the genes. Results revealed that the highest number of DEGs (4735) between the two genotypes was detected after 24 h of infection. Interestingly, it was found that several of the DEGs between the WT and <i<OsF3H</i< under control conditions were also differentially expressed in <i<OsF3H</i< in response to WBPH infestation. These results indicate that significant differences in gene expression between the “<i<OxF3H</i<” and “WT” exist as the infection time increases. Many of these DEGs were related to oxidoreductase activity, response to stress, salicylic acid biosynthesis, metabolic process, defense response to pathogen, cellular response to toxic substance, and regulation of hormone levels. Moreover, genes involved in salicylic acid (SA) and ethylene (Et) biosynthesis were upregulated in <i<OxF3H</i< plants, while jasmonic acid (JA), brassinosteroid (Br), and abscisic acid (ABA) signaling pathways were found downregulated in <i<OxF3H</i< plants during WBPH infestation. Interestingly, many DEGs related to pathogenesis, such as <i<OsPR1</i<, <i<OsPR1b</i<, <i<OsNPR1</i<, <i<OsNPR3,</i< and <i<OsNPR5,</i< were found to be significantly upregulated in <i<OxF3H</i< plants. Additionally, genes related to the MAPKs pathway and about 30 <i<WRKY</i< genes involved in different pathways were upregulated in <i<OxF3H</i< plants after WBPH infestation. This suggests that overexpression of the <i<OxF3H</i< gene leads to multiple transcriptomic changes and impacts plant hormones and pathogenic-related and secondary-metabolites-related genes, enhancing the plant’s resistance to WBPH infestation. <i<Oryza sativa</i< pathogen defense system salicylic acid jasmonic acid transcriptomics Biology (General) Chemistry Sajjad Asaf verfasserin aut Lubna verfasserin aut Saleem Asif verfasserin aut Eun-Gyeong Kim verfasserin aut Yoon-Hee Jang verfasserin aut Nari Kim verfasserin aut Ahmed Al-Harrasi verfasserin aut Gang-Seob Lee verfasserin aut Kyung-Min Kim verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 23, p 15308 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:23, p 15308 https://doi.org/10.3390/ijms232315308 kostenfrei https://doaj.org/article/2dedc68170fa40e0bfff23c8f7c8272d kostenfrei https://www.mdpi.com/1422-0067/23/23/15308 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 23, p 15308
language	English
source	In International Journal of Molecular Sciences 23(2022), 23, p 15308 volume:23 year:2022 number:23, p 15308
sourceStr	In International Journal of Molecular Sciences 23(2022), 23, p 15308 volume:23 year:2022 number:23, p 15308
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<Oryza sativa</i< pathogen defense system salicylic acid jasmonic acid transcriptomics Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Rahmatullah Jan @@aut@@ Sajjad Asaf @@aut@@ Lubna @@aut@@ Saleem Asif @@aut@@ Eun-Gyeong Kim @@aut@@ Yoon-Hee Jang @@aut@@ Nari Kim @@aut@@ Ahmed Al-Harrasi @@aut@@ Gang-Seob Lee @@aut@@ Kyung-Min Kim @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	316340715
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We previously developed the WBPH-resistant rice cultivar “<i<OxF3H</i<” by overexpressing the <i<OsF3H</i< gene. Although there was a higher accumulation of the flavonoids kaempferol (Kr) and quercetin (Qu) as well as salicylic acid (SA) in <i<OxF3H</i< transgenic (<i<OsF3H</i< or Trans) plants compared to the wild type (WT), it is still unclear how Os<i<F3H</i< overexpression affects these WBPH resistant-related changes in gene expression in <i<OxF3H</i< plants. In this study, we analyze RNA-seq data from <i<OxF3H</i< and WT at several points (0 h, 3 h, 12 h, and 24 h) after WBPH infection to explain how overall changes in gene expression happen in these two cultivars. RT-qPCR further validated a number of the genes. Results revealed that the highest number of DEGs (4735) between the two genotypes was detected after 24 h of infection. Interestingly, it was found that several of the DEGs between the WT and <i<OsF3H</i< under control conditions were also differentially expressed in <i<OsF3H</i< in response to WBPH infestation. These results indicate that significant differences in gene expression between the “<i<OxF3H</i<” and “WT” exist as the infection time increases. Many of these DEGs were related to oxidoreductase activity, response to stress, salicylic acid biosynthesis, metabolic process, defense response to pathogen, cellular response to toxic substance, and regulation of hormone levels. Moreover, genes involved in salicylic acid (SA) and ethylene (Et) biosynthesis were upregulated in <i<OxF3H</i< plants, while jasmonic acid (JA), brassinosteroid (Br), and abscisic acid (ABA) signaling pathways were found downregulated in <i<OxF3H</i< plants during WBPH infestation. 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callnumber-first	Q - Science
author	Rahmatullah Jan
spellingShingle	Rahmatullah Jan misc QH301-705.5 misc QD1-999 misc <i<Oryza sativa</i< misc pathogen misc defense system misc salicylic acid misc jasmonic acid misc transcriptomics misc Biology (General) misc Chemistry Enhancing the Expression of the <i<OsF3H</i< Gene in <i<Oryza sativa</i< Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance
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topic_title	QH301-705.5 QD1-999 Enhancing the Expression of the <i<OsF3H</i< Gene in <i<Oryza sativa</i< Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance <i<Oryza sativa</i< pathogen defense system salicylic acid jasmonic acid transcriptomics
topic	misc QH301-705.5 misc QD1-999 misc <i<Oryza sativa</i< misc pathogen misc defense system misc salicylic acid misc jasmonic acid misc transcriptomics misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc <i<Oryza sativa</i< misc pathogen misc defense system misc salicylic acid misc jasmonic acid misc transcriptomics misc Biology (General) misc Chemistry
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title	Enhancing the Expression of the <i<OsF3H</i< Gene in <i<Oryza sativa</i< Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance
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title_full	Enhancing the Expression of the <i<OsF3H</i< Gene in <i<Oryza sativa</i< Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance
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author_browse	Rahmatullah Jan Sajjad Asaf Lubna Saleem Asif Eun-Gyeong Kim Yoon-Hee Jang Nari Kim Ahmed Al-Harrasi Gang-Seob Lee Kyung-Min Kim
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title_sort	enhancing the expression of the <i<osf3h</i< gene in <i<oryza sativa</i< leads to the regulation of multiple biosynthetic pathways and transcriptomic changes that influence insect resistance
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title_auth	Enhancing the Expression of the <i<OsF3H</i< Gene in <i<Oryza sativa</i< Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance
abstract	The white-backed planthopper (WBPH) is a major pest of rice crops and causes severe loss of yield. We previously developed the WBPH-resistant rice cultivar “<i<OxF3H</i<” by overexpressing the <i<OsF3H</i< gene. Although there was a higher accumulation of the flavonoids kaempferol (Kr) and quercetin (Qu) as well as salicylic acid (SA) in <i<OxF3H</i< transgenic (<i<OsF3H</i< or Trans) plants compared to the wild type (WT), it is still unclear how Os<i<F3H</i< overexpression affects these WBPH resistant-related changes in gene expression in <i<OxF3H</i< plants. In this study, we analyze RNA-seq data from <i<OxF3H</i< and WT at several points (0 h, 3 h, 12 h, and 24 h) after WBPH infection to explain how overall changes in gene expression happen in these two cultivars. RT-qPCR further validated a number of the genes. Results revealed that the highest number of DEGs (4735) between the two genotypes was detected after 24 h of infection. Interestingly, it was found that several of the DEGs between the WT and <i<OsF3H</i< under control conditions were also differentially expressed in <i<OsF3H</i< in response to WBPH infestation. These results indicate that significant differences in gene expression between the “<i<OxF3H</i<” and “WT” exist as the infection time increases. Many of these DEGs were related to oxidoreductase activity, response to stress, salicylic acid biosynthesis, metabolic process, defense response to pathogen, cellular response to toxic substance, and regulation of hormone levels. Moreover, genes involved in salicylic acid (SA) and ethylene (Et) biosynthesis were upregulated in <i<OxF3H</i< plants, while jasmonic acid (JA), brassinosteroid (Br), and abscisic acid (ABA) signaling pathways were found downregulated in <i<OxF3H</i< plants during WBPH infestation. Interestingly, many DEGs related to pathogenesis, such as <i<OsPR1</i<, <i<OsPR1b</i<, <i<OsNPR1</i<, <i<OsNPR3,</i< and <i<OsNPR5,</i< were found to be significantly upregulated in <i<OxF3H</i< plants. Additionally, genes related to the MAPKs pathway and about 30 <i<WRKY</i< genes involved in different pathways were upregulated in <i<OxF3H</i< plants after WBPH infestation. This suggests that overexpression of the <i<OxF3H</i< gene leads to multiple transcriptomic changes and impacts plant hormones and pathogenic-related and secondary-metabolites-related genes, enhancing the plant’s resistance to WBPH infestation.
abstractGer	The white-backed planthopper (WBPH) is a major pest of rice crops and causes severe loss of yield. We previously developed the WBPH-resistant rice cultivar “<i<OxF3H</i<” by overexpressing the <i<OsF3H</i< gene. Although there was a higher accumulation of the flavonoids kaempferol (Kr) and quercetin (Qu) as well as salicylic acid (SA) in <i<OxF3H</i< transgenic (<i<OsF3H</i< or Trans) plants compared to the wild type (WT), it is still unclear how Os<i<F3H</i< overexpression affects these WBPH resistant-related changes in gene expression in <i<OxF3H</i< plants. In this study, we analyze RNA-seq data from <i<OxF3H</i< and WT at several points (0 h, 3 h, 12 h, and 24 h) after WBPH infection to explain how overall changes in gene expression happen in these two cultivars. RT-qPCR further validated a number of the genes. Results revealed that the highest number of DEGs (4735) between the two genotypes was detected after 24 h of infection. Interestingly, it was found that several of the DEGs between the WT and <i<OsF3H</i< under control conditions were also differentially expressed in <i<OsF3H</i< in response to WBPH infestation. These results indicate that significant differences in gene expression between the “<i<OxF3H</i<” and “WT” exist as the infection time increases. Many of these DEGs were related to oxidoreductase activity, response to stress, salicylic acid biosynthesis, metabolic process, defense response to pathogen, cellular response to toxic substance, and regulation of hormone levels. Moreover, genes involved in salicylic acid (SA) and ethylene (Et) biosynthesis were upregulated in <i<OxF3H</i< plants, while jasmonic acid (JA), brassinosteroid (Br), and abscisic acid (ABA) signaling pathways were found downregulated in <i<OxF3H</i< plants during WBPH infestation. Interestingly, many DEGs related to pathogenesis, such as <i<OsPR1</i<, <i<OsPR1b</i<, <i<OsNPR1</i<, <i<OsNPR3,</i< and <i<OsNPR5,</i< were found to be significantly upregulated in <i<OxF3H</i< plants. Additionally, genes related to the MAPKs pathway and about 30 <i<WRKY</i< genes involved in different pathways were upregulated in <i<OxF3H</i< plants after WBPH infestation. This suggests that overexpression of the <i<OxF3H</i< gene leads to multiple transcriptomic changes and impacts plant hormones and pathogenic-related and secondary-metabolites-related genes, enhancing the plant’s resistance to WBPH infestation.
abstract_unstemmed	The white-backed planthopper (WBPH) is a major pest of rice crops and causes severe loss of yield. We previously developed the WBPH-resistant rice cultivar “<i<OxF3H</i<” by overexpressing the <i<OsF3H</i< gene. Although there was a higher accumulation of the flavonoids kaempferol (Kr) and quercetin (Qu) as well as salicylic acid (SA) in <i<OxF3H</i< transgenic (<i<OsF3H</i< or Trans) plants compared to the wild type (WT), it is still unclear how Os<i<F3H</i< overexpression affects these WBPH resistant-related changes in gene expression in <i<OxF3H</i< plants. In this study, we analyze RNA-seq data from <i<OxF3H</i< and WT at several points (0 h, 3 h, 12 h, and 24 h) after WBPH infection to explain how overall changes in gene expression happen in these two cultivars. RT-qPCR further validated a number of the genes. Results revealed that the highest number of DEGs (4735) between the two genotypes was detected after 24 h of infection. Interestingly, it was found that several of the DEGs between the WT and <i<OsF3H</i< under control conditions were also differentially expressed in <i<OsF3H</i< in response to WBPH infestation. These results indicate that significant differences in gene expression between the “<i<OxF3H</i<” and “WT” exist as the infection time increases. Many of these DEGs were related to oxidoreductase activity, response to stress, salicylic acid biosynthesis, metabolic process, defense response to pathogen, cellular response to toxic substance, and regulation of hormone levels. Moreover, genes involved in salicylic acid (SA) and ethylene (Et) biosynthesis were upregulated in <i<OxF3H</i< plants, while jasmonic acid (JA), brassinosteroid (Br), and abscisic acid (ABA) signaling pathways were found downregulated in <i<OxF3H</i< plants during WBPH infestation. Interestingly, many DEGs related to pathogenesis, such as <i<OsPR1</i<, <i<OsPR1b</i<, <i<OsNPR1</i<, <i<OsNPR3,</i< and <i<OsNPR5,</i< were found to be significantly upregulated in <i<OxF3H</i< plants. Additionally, genes related to the MAPKs pathway and about 30 <i<WRKY</i< genes involved in different pathways were upregulated in <i<OxF3H</i< plants after WBPH infestation. This suggests that overexpression of the <i<OxF3H</i< gene leads to multiple transcriptomic changes and impacts plant hormones and pathogenic-related and secondary-metabolites-related genes, enhancing the plant’s resistance to WBPH infestation.
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title_short	Enhancing the Expression of the <i<OsF3H</i< Gene in <i<Oryza sativa</i< Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance
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up_date	2024-07-03T15:51:57.158Z
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fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ085619191</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240414155504.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230311s2022 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3390/ijms232315308</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ085619191</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ2dedc68170fa40e0bfff23c8f7c8272d</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH301-705.5</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QD1-999</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Rahmatullah Jan</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Enhancing the Expression of the <i<OsF3H</i< Gene in <i<Oryza sativa</i< Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2022</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The white-backed planthopper (WBPH) is a major pest of rice crops and causes severe loss of yield. We previously developed the WBPH-resistant rice cultivar “<i<OxF3H</i<” by overexpressing the <i<OsF3H</i< gene. Although there was a higher accumulation of the flavonoids kaempferol (Kr) and quercetin (Qu) as well as salicylic acid (SA) in <i<OxF3H</i< transgenic (<i<OsF3H</i< or Trans) plants compared to the wild type (WT), it is still unclear how Os<i<F3H</i< overexpression affects these WBPH resistant-related changes in gene expression in <i<OxF3H</i< plants. In this study, we analyze RNA-seq data from <i<OxF3H</i< and WT at several points (0 h, 3 h, 12 h, and 24 h) after WBPH infection to explain how overall changes in gene expression happen in these two cultivars. RT-qPCR further validated a number of the genes. Results revealed that the highest number of DEGs (4735) between the two genotypes was detected after 24 h of infection. Interestingly, it was found that several of the DEGs between the WT and <i<OsF3H</i< under control conditions were also differentially expressed in <i<OsF3H</i< in response to WBPH infestation. These results indicate that significant differences in gene expression between the “<i<OxF3H</i<” and “WT” exist as the infection time increases. Many of these DEGs were related to oxidoreductase activity, response to stress, salicylic acid biosynthesis, metabolic process, defense response to pathogen, cellular response to toxic substance, and regulation of hormone levels. Moreover, genes involved in salicylic acid (SA) and ethylene (Et) biosynthesis were upregulated in <i<OxF3H</i< plants, while jasmonic acid (JA), brassinosteroid (Br), and abscisic acid (ABA) signaling pathways were found downregulated in <i<OxF3H</i< plants during WBPH infestation. Interestingly, many DEGs related to pathogenesis, such as <i<OsPR1</i<, <i<OsPR1b</i<, <i<OsNPR1</i<, <i<OsNPR3,</i< and <i<OsNPR5,</i< were found to be significantly upregulated in <i<OxF3H</i< plants. Additionally, genes related to the MAPKs pathway and about 30 <i<WRKY</i< genes involved in different pathways were upregulated in <i<OxF3H</i< plants after WBPH infestation. 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Enhancing the Expression of the <i<OsF3H</i< Gene in <i<Oryza sativa</i< Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance

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