Exploring Secondary Metabolite Profiles of <i<Stachybotrys</i< spp. by LC-MS/MS

The genus <i<Stachybotrys</i< produces a broad diversity of secondary metabolites, including macrocyclic trichothecenes, atranones, and phenylspirodrimanes. Although the class of the phenylspirodrimanes is the major one and consists of a multitude of metabolites bearing various structura...
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Autor*in:	Annika Jagels [verfasserIn] Viktoria Lindemann [verfasserIn] Sebastian Ulrich [verfasserIn] Christoph Gottschalk [verfasserIn] Benedikt Cramer [verfasserIn] Florian Hübner [verfasserIn] Manfred Gareis [verfasserIn] Hans-Ulrich Humpf [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	metabolite profiles LC-MS/MS satratoxins phenylspirodrimanes stachybotrychromenes biosynthetic production

Übergeordnetes Werk:	In: Toxins - MDPI AG, 2010, 11(2019), 3, p 133
Übergeordnetes Werk:	volume:11 ; year:2019 ; number:3, p 133

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/toxins11030133

Katalog-ID:	DOAJ085662542

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allfields	10.3390/toxins11030133 doi (DE-627)DOAJ085662542 (DE-599)DOAJ5cd15a5d4cce4b37935739599ce29363 DE-627 ger DE-627 rakwb eng Annika Jagels verfasserin aut Exploring Secondary Metabolite Profiles of <i<Stachybotrys</i< spp. by LC-MS/MS 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The genus <i<Stachybotrys</i< produces a broad diversity of secondary metabolites, including macrocyclic trichothecenes, atranones, and phenylspirodrimanes. Although the class of the phenylspirodrimanes is the major one and consists of a multitude of metabolites bearing various structural modifications, few investigations have been carried out. Thus, the presented study deals with the quantitative determination of several secondary metabolites produced by distinct <i<Stachybotrys</i< species for comparison of their metabolite profiles. For that purpose, 15 of the primarily produced secondary metabolites were isolated from fungal cultures and structurally characterized in order to be used as analytical standards for the development of an LC-MS/MS multimethod. The developed method was applied to the analysis of micro-scale extracts from 5 different <i<Stachybotrys</i< strains, which were cultured on different media. In that process, spontaneous dialdehyde/lactone isomerization was observed for some of the isolated secondary metabolites, and novel stachybotrychromenes were quantitatively investigated for the first time. The metabolite profiles of <i<Stachybotrys</i< species are considerably influenced by time of growth and substrate availability, as well as the individual biosynthetic potential of the respective species. Regarding the reported adverse effects associated with <i<Stachybotrys</i< growth in building environments, combinatory effects of the investigated secondary metabolites should be addressed and the role of the phenylspirodrimanes re-evaluated in future research. <i<Stachybotrys</i< spp. metabolite profiles LC-MS/MS satratoxins phenylspirodrimanes stachybotrychromenes biosynthetic production Medicine R Viktoria Lindemann verfasserin aut Sebastian Ulrich verfasserin aut Christoph Gottschalk verfasserin aut Benedikt Cramer verfasserin aut Florian Hübner verfasserin aut Manfred Gareis verfasserin aut Hans-Ulrich Humpf verfasserin aut In Toxins MDPI AG, 2010 11(2019), 3, p 133 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:11 year:2019 number:3, p 133 https://doi.org/10.3390/toxins11030133 kostenfrei https://doaj.org/article/5cd15a5d4cce4b37935739599ce29363 kostenfrei https://www.mdpi.com/2072-6651/11/3/133 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2019 3, p 133
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allfieldsSound	10.3390/toxins11030133 doi (DE-627)DOAJ085662542 (DE-599)DOAJ5cd15a5d4cce4b37935739599ce29363 DE-627 ger DE-627 rakwb eng Annika Jagels verfasserin aut Exploring Secondary Metabolite Profiles of <i<Stachybotrys</i< spp. by LC-MS/MS 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The genus <i<Stachybotrys</i< produces a broad diversity of secondary metabolites, including macrocyclic trichothecenes, atranones, and phenylspirodrimanes. Although the class of the phenylspirodrimanes is the major one and consists of a multitude of metabolites bearing various structural modifications, few investigations have been carried out. Thus, the presented study deals with the quantitative determination of several secondary metabolites produced by distinct <i<Stachybotrys</i< species for comparison of their metabolite profiles. For that purpose, 15 of the primarily produced secondary metabolites were isolated from fungal cultures and structurally characterized in order to be used as analytical standards for the development of an LC-MS/MS multimethod. The developed method was applied to the analysis of micro-scale extracts from 5 different <i<Stachybotrys</i< strains, which were cultured on different media. In that process, spontaneous dialdehyde/lactone isomerization was observed for some of the isolated secondary metabolites, and novel stachybotrychromenes were quantitatively investigated for the first time. The metabolite profiles of <i<Stachybotrys</i< species are considerably influenced by time of growth and substrate availability, as well as the individual biosynthetic potential of the respective species. Regarding the reported adverse effects associated with <i<Stachybotrys</i< growth in building environments, combinatory effects of the investigated secondary metabolites should be addressed and the role of the phenylspirodrimanes re-evaluated in future research. <i<Stachybotrys</i< spp. metabolite profiles LC-MS/MS satratoxins phenylspirodrimanes stachybotrychromenes biosynthetic production Medicine R Viktoria Lindemann verfasserin aut Sebastian Ulrich verfasserin aut Christoph Gottschalk verfasserin aut Benedikt Cramer verfasserin aut Florian Hübner verfasserin aut Manfred Gareis verfasserin aut Hans-Ulrich Humpf verfasserin aut In Toxins MDPI AG, 2010 11(2019), 3, p 133 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:11 year:2019 number:3, p 133 https://doi.org/10.3390/toxins11030133 kostenfrei https://doaj.org/article/5cd15a5d4cce4b37935739599ce29363 kostenfrei https://www.mdpi.com/2072-6651/11/3/133 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2019 3, p 133
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author	Annika Jagels
spellingShingle	Annika Jagels misc <i<Stachybotrys</i< spp. misc metabolite profiles misc LC-MS/MS misc satratoxins misc phenylspirodrimanes misc stachybotrychromenes misc biosynthetic production misc Medicine misc R Exploring Secondary Metabolite Profiles of <i<Stachybotrys</i< spp. by LC-MS/MS
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topic_title	Exploring Secondary Metabolite Profiles of <i<Stachybotrys</i< spp. by LC-MS/MS <i<Stachybotrys</i< spp metabolite profiles LC-MS/MS satratoxins phenylspirodrimanes stachybotrychromenes biosynthetic production
topic	misc <i<Stachybotrys</i< spp. misc metabolite profiles misc LC-MS/MS misc satratoxins misc phenylspirodrimanes misc stachybotrychromenes misc biosynthetic production misc Medicine misc R
topic_unstemmed	misc <i<Stachybotrys</i< spp. misc metabolite profiles misc LC-MS/MS misc satratoxins misc phenylspirodrimanes misc stachybotrychromenes misc biosynthetic production misc Medicine misc R
topic_browse	misc <i<Stachybotrys</i< spp. misc metabolite profiles misc LC-MS/MS misc satratoxins misc phenylspirodrimanes misc stachybotrychromenes misc biosynthetic production misc Medicine misc R
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title	Exploring Secondary Metabolite Profiles of <i<Stachybotrys</i< spp. by LC-MS/MS
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title_full	Exploring Secondary Metabolite Profiles of <i<Stachybotrys</i< spp. by LC-MS/MS
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title_sort	exploring secondary metabolite profiles of <i<stachybotrys</i< spp. by lc-ms/ms
title_auth	Exploring Secondary Metabolite Profiles of <i<Stachybotrys</i< spp. by LC-MS/MS
abstract	The genus <i<Stachybotrys</i< produces a broad diversity of secondary metabolites, including macrocyclic trichothecenes, atranones, and phenylspirodrimanes. Although the class of the phenylspirodrimanes is the major one and consists of a multitude of metabolites bearing various structural modifications, few investigations have been carried out. Thus, the presented study deals with the quantitative determination of several secondary metabolites produced by distinct <i<Stachybotrys</i< species for comparison of their metabolite profiles. For that purpose, 15 of the primarily produced secondary metabolites were isolated from fungal cultures and structurally characterized in order to be used as analytical standards for the development of an LC-MS/MS multimethod. The developed method was applied to the analysis of micro-scale extracts from 5 different <i<Stachybotrys</i< strains, which were cultured on different media. In that process, spontaneous dialdehyde/lactone isomerization was observed for some of the isolated secondary metabolites, and novel stachybotrychromenes were quantitatively investigated for the first time. The metabolite profiles of <i<Stachybotrys</i< species are considerably influenced by time of growth and substrate availability, as well as the individual biosynthetic potential of the respective species. Regarding the reported adverse effects associated with <i<Stachybotrys</i< growth in building environments, combinatory effects of the investigated secondary metabolites should be addressed and the role of the phenylspirodrimanes re-evaluated in future research.
abstractGer	The genus <i<Stachybotrys</i< produces a broad diversity of secondary metabolites, including macrocyclic trichothecenes, atranones, and phenylspirodrimanes. Although the class of the phenylspirodrimanes is the major one and consists of a multitude of metabolites bearing various structural modifications, few investigations have been carried out. Thus, the presented study deals with the quantitative determination of several secondary metabolites produced by distinct <i<Stachybotrys</i< species for comparison of their metabolite profiles. For that purpose, 15 of the primarily produced secondary metabolites were isolated from fungal cultures and structurally characterized in order to be used as analytical standards for the development of an LC-MS/MS multimethod. The developed method was applied to the analysis of micro-scale extracts from 5 different <i<Stachybotrys</i< strains, which were cultured on different media. In that process, spontaneous dialdehyde/lactone isomerization was observed for some of the isolated secondary metabolites, and novel stachybotrychromenes were quantitatively investigated for the first time. The metabolite profiles of <i<Stachybotrys</i< species are considerably influenced by time of growth and substrate availability, as well as the individual biosynthetic potential of the respective species. Regarding the reported adverse effects associated with <i<Stachybotrys</i< growth in building environments, combinatory effects of the investigated secondary metabolites should be addressed and the role of the phenylspirodrimanes re-evaluated in future research.
abstract_unstemmed	The genus <i<Stachybotrys</i< produces a broad diversity of secondary metabolites, including macrocyclic trichothecenes, atranones, and phenylspirodrimanes. Although the class of the phenylspirodrimanes is the major one and consists of a multitude of metabolites bearing various structural modifications, few investigations have been carried out. Thus, the presented study deals with the quantitative determination of several secondary metabolites produced by distinct <i<Stachybotrys</i< species for comparison of their metabolite profiles. For that purpose, 15 of the primarily produced secondary metabolites were isolated from fungal cultures and structurally characterized in order to be used as analytical standards for the development of an LC-MS/MS multimethod. The developed method was applied to the analysis of micro-scale extracts from 5 different <i<Stachybotrys</i< strains, which were cultured on different media. In that process, spontaneous dialdehyde/lactone isomerization was observed for some of the isolated secondary metabolites, and novel stachybotrychromenes were quantitatively investigated for the first time. The metabolite profiles of <i<Stachybotrys</i< species are considerably influenced by time of growth and substrate availability, as well as the individual biosynthetic potential of the respective species. Regarding the reported adverse effects associated with <i<Stachybotrys</i< growth in building environments, combinatory effects of the investigated secondary metabolites should be addressed and the role of the phenylspirodrimanes re-evaluated in future research.
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title_short	Exploring Secondary Metabolite Profiles of <i<Stachybotrys</i< spp. by LC-MS/MS
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Exploring Secondary Metabolite Profiles of <i<Stachybotrys</i< spp. by LC-MS/MS

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