Human monocyte-derived suppressive cells (HuMoSC) for cell therapy in giant cell arteritis

IntroductionThe pathogenesis of Giant Cell Arteritis (GCA) relies on vascular inflammation and vascular remodeling, the latter being poorly controlled by current treatments.MethodsThis study aimed to evaluate the effect of a novel cell therapy, Human Monocyte-derived Suppressor Cells (HuMoSC), on in...
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Autor*in:	Maxime Samson [verfasserIn] Coraline Genet [verfasserIn] Marc Corbera-Bellalta [verfasserIn] Hélène Greigert [verfasserIn] Georgina Espígol-Frigolé [verfasserIn] Claire Gérard [verfasserIn] Claudie Cladière [verfasserIn] Roser Alba-Rovira [verfasserIn] Marion Ciudad [verfasserIn] Pierre-Henry Gabrielle [verfasserIn] Catherine Creuzot-Garcher [verfasserIn] Georges Tarris [verfasserIn] Laurent Martin [verfasserIn] Philippe Saas [verfasserIn] Sylvain Audia [verfasserIn] Bernard Bonnotte [verfasserIn] Maria C. Cid [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	suppressive cells giant cell arteritis treatment cellular therapy vascular remodeling

Übergeordnetes Werk:	In: Frontiers in Immunology - Frontiers Media S.A., 2011, 14(2023)
Übergeordnetes Werk:	volume:14 ; year:2023

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fimmu.2023.1137794

Katalog-ID:	DOAJ086253271

Internformat


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520			\|a IntroductionThe pathogenesis of Giant Cell Arteritis (GCA) relies on vascular inflammation and vascular remodeling, the latter being poorly controlled by current treatments.MethodsThis study aimed to evaluate the effect of a novel cell therapy, Human Monocyte-derived Suppressor Cells (HuMoSC), on inflammation and vascular remodeling to improve GCA treatment. Fragments of temporal arteries (TAs) from GCA patients were cultured alone or in the presence of HuMoSCs or their supernatant. After five days, mRNA expression was measured in the TAs and proteins were measured in culture supernatant. The proliferation and migration capacity of vascular smooth muscle cells (VSMCs) were also analyzed with or without HuMoSC supernatant. ResultsTranscripts of genes implicated in vascular inflammation (CCL2, CCR2, CXCR3, HLADR), vascular remodeling (PDGF, PDGFR), angiogenesis (VEGF) and extracellular matrix composition (COL1A1, COL3A1 and FN1) were decreased in arteries treated with HuMoSCs or their supernatant. Likewise, concentrations of collagen-1 and VEGF were lower in the supernatants of TAs cultivated with HuMoSCs. In the presence of PDGF, the proliferation and migration of VSMCs were both decreased after treatment with HuMoSC supernatant. Study of the PDGF pathway suggests that HuMoSCs act through inhibition of mTOR activity. Finally, we show that HuMoSCs could be recruited in the arterial wall through the implication of CCR5 and its ligands.ConclusionAltogether, our results suggest that HuMoSCs or their supernatant could be useful to decrease vascular in flammation and remodeling in GCA, the latter being an unmet need in GCA treatment.
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653		0	\|a Immunologic diseases. Allergy
700	0		\|a Maxime Samson \|e verfasserin \|4 aut
700	0		\|a Maxime Samson \|e verfasserin \|4 aut
700	0		\|a Coraline Genet \|e verfasserin \|4 aut
700	0		\|a Marc Corbera-Bellalta \|e verfasserin \|4 aut
700	0		\|a Hélène Greigert \|e verfasserin \|4 aut
700	0		\|a Hélène Greigert \|e verfasserin \|4 aut
700	0		\|a Georgina Espígol-Frigolé \|e verfasserin \|4 aut
700	0		\|a Claire Gérard \|e verfasserin \|4 aut
700	0		\|a Claudie Cladière \|e verfasserin \|4 aut
700	0		\|a Roser Alba-Rovira \|e verfasserin \|4 aut
700	0		\|a Marion Ciudad \|e verfasserin \|4 aut
700	0		\|a Pierre-Henry Gabrielle \|e verfasserin \|4 aut
700	0		\|a Catherine Creuzot-Garcher \|e verfasserin \|4 aut
700	0		\|a Georges Tarris \|e verfasserin \|4 aut
700	0		\|a Laurent Martin \|e verfasserin \|4 aut
700	0		\|a Philippe Saas \|e verfasserin \|4 aut
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700	0		\|a Sylvain Audia \|e verfasserin \|4 aut
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700	0		\|a Bernard Bonnotte \|e verfasserin \|4 aut
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700	0		\|a Maria C. Cid \|e verfasserin \|4 aut
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allfields	10.3389/fimmu.2023.1137794 doi (DE-627)DOAJ086253271 (DE-599)DOAJad20cec6940f4fb28020e1bb5f5f6a43 DE-627 ger DE-627 rakwb eng RC581-607 Maxime Samson verfasserin aut Human monocyte-derived suppressive cells (HuMoSC) for cell therapy in giant cell arteritis 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier IntroductionThe pathogenesis of Giant Cell Arteritis (GCA) relies on vascular inflammation and vascular remodeling, the latter being poorly controlled by current treatments.MethodsThis study aimed to evaluate the effect of a novel cell therapy, Human Monocyte-derived Suppressor Cells (HuMoSC), on inflammation and vascular remodeling to improve GCA treatment. Fragments of temporal arteries (TAs) from GCA patients were cultured alone or in the presence of HuMoSCs or their supernatant. After five days, mRNA expression was measured in the TAs and proteins were measured in culture supernatant. The proliferation and migration capacity of vascular smooth muscle cells (VSMCs) were also analyzed with or without HuMoSC supernatant. ResultsTranscripts of genes implicated in vascular inflammation (CCL2, CCR2, CXCR3, HLADR), vascular remodeling (PDGF, PDGFR), angiogenesis (VEGF) and extracellular matrix composition (COL1A1, COL3A1 and FN1) were decreased in arteries treated with HuMoSCs or their supernatant. Likewise, concentrations of collagen-1 and VEGF were lower in the supernatants of TAs cultivated with HuMoSCs. In the presence of PDGF, the proliferation and migration of VSMCs were both decreased after treatment with HuMoSC supernatant. Study of the PDGF pathway suggests that HuMoSCs act through inhibition of mTOR activity. Finally, we show that HuMoSCs could be recruited in the arterial wall through the implication of CCR5 and its ligands.ConclusionAltogether, our results suggest that HuMoSCs or their supernatant could be useful to decrease vascular in flammation and remodeling in GCA, the latter being an unmet need in GCA treatment. suppressive cells giant cell arteritis treatment cellular therapy vascular remodeling Immunologic diseases. Allergy Maxime Samson verfasserin aut Maxime Samson verfasserin aut Coraline Genet verfasserin aut Marc Corbera-Bellalta verfasserin aut Hélène Greigert verfasserin aut Hélène Greigert verfasserin aut Georgina Espígol-Frigolé verfasserin aut Claire Gérard verfasserin aut Claudie Cladière verfasserin aut Roser Alba-Rovira verfasserin aut Marion Ciudad verfasserin aut Pierre-Henry Gabrielle verfasserin aut Catherine Creuzot-Garcher verfasserin aut Georges Tarris verfasserin aut Laurent Martin verfasserin aut Philippe Saas verfasserin aut Philippe Saas verfasserin aut Sylvain Audia verfasserin aut Sylvain Audia verfasserin aut Bernard Bonnotte verfasserin aut Bernard Bonnotte verfasserin aut Maria C. Cid verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 14(2023) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:14 year:2023 https://doi.org/10.3389/fimmu.2023.1137794 kostenfrei https://doaj.org/article/ad20cec6940f4fb28020e1bb5f5f6a43 kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2023.1137794/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023
spelling	10.3389/fimmu.2023.1137794 doi (DE-627)DOAJ086253271 (DE-599)DOAJad20cec6940f4fb28020e1bb5f5f6a43 DE-627 ger DE-627 rakwb eng RC581-607 Maxime Samson verfasserin aut Human monocyte-derived suppressive cells (HuMoSC) for cell therapy in giant cell arteritis 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier IntroductionThe pathogenesis of Giant Cell Arteritis (GCA) relies on vascular inflammation and vascular remodeling, the latter being poorly controlled by current treatments.MethodsThis study aimed to evaluate the effect of a novel cell therapy, Human Monocyte-derived Suppressor Cells (HuMoSC), on inflammation and vascular remodeling to improve GCA treatment. Fragments of temporal arteries (TAs) from GCA patients were cultured alone or in the presence of HuMoSCs or their supernatant. After five days, mRNA expression was measured in the TAs and proteins were measured in culture supernatant. The proliferation and migration capacity of vascular smooth muscle cells (VSMCs) were also analyzed with or without HuMoSC supernatant. ResultsTranscripts of genes implicated in vascular inflammation (CCL2, CCR2, CXCR3, HLADR), vascular remodeling (PDGF, PDGFR), angiogenesis (VEGF) and extracellular matrix composition (COL1A1, COL3A1 and FN1) were decreased in arteries treated with HuMoSCs or their supernatant. Likewise, concentrations of collagen-1 and VEGF were lower in the supernatants of TAs cultivated with HuMoSCs. In the presence of PDGF, the proliferation and migration of VSMCs were both decreased after treatment with HuMoSC supernatant. Study of the PDGF pathway suggests that HuMoSCs act through inhibition of mTOR activity. Finally, we show that HuMoSCs could be recruited in the arterial wall through the implication of CCR5 and its ligands.ConclusionAltogether, our results suggest that HuMoSCs or their supernatant could be useful to decrease vascular in flammation and remodeling in GCA, the latter being an unmet need in GCA treatment. suppressive cells giant cell arteritis treatment cellular therapy vascular remodeling Immunologic diseases. Allergy Maxime Samson verfasserin aut Maxime Samson verfasserin aut Coraline Genet verfasserin aut Marc Corbera-Bellalta verfasserin aut Hélène Greigert verfasserin aut Hélène Greigert verfasserin aut Georgina Espígol-Frigolé verfasserin aut Claire Gérard verfasserin aut Claudie Cladière verfasserin aut Roser Alba-Rovira verfasserin aut Marion Ciudad verfasserin aut Pierre-Henry Gabrielle verfasserin aut Catherine Creuzot-Garcher verfasserin aut Georges Tarris verfasserin aut Laurent Martin verfasserin aut Philippe Saas verfasserin aut Philippe Saas verfasserin aut Sylvain Audia verfasserin aut Sylvain Audia verfasserin aut Bernard Bonnotte verfasserin aut Bernard Bonnotte verfasserin aut Maria C. Cid verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 14(2023) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:14 year:2023 https://doi.org/10.3389/fimmu.2023.1137794 kostenfrei https://doaj.org/article/ad20cec6940f4fb28020e1bb5f5f6a43 kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2023.1137794/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023
allfields_unstemmed	10.3389/fimmu.2023.1137794 doi (DE-627)DOAJ086253271 (DE-599)DOAJad20cec6940f4fb28020e1bb5f5f6a43 DE-627 ger DE-627 rakwb eng RC581-607 Maxime Samson verfasserin aut Human monocyte-derived suppressive cells (HuMoSC) for cell therapy in giant cell arteritis 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier IntroductionThe pathogenesis of Giant Cell Arteritis (GCA) relies on vascular inflammation and vascular remodeling, the latter being poorly controlled by current treatments.MethodsThis study aimed to evaluate the effect of a novel cell therapy, Human Monocyte-derived Suppressor Cells (HuMoSC), on inflammation and vascular remodeling to improve GCA treatment. Fragments of temporal arteries (TAs) from GCA patients were cultured alone or in the presence of HuMoSCs or their supernatant. After five days, mRNA expression was measured in the TAs and proteins were measured in culture supernatant. The proliferation and migration capacity of vascular smooth muscle cells (VSMCs) were also analyzed with or without HuMoSC supernatant. ResultsTranscripts of genes implicated in vascular inflammation (CCL2, CCR2, CXCR3, HLADR), vascular remodeling (PDGF, PDGFR), angiogenesis (VEGF) and extracellular matrix composition (COL1A1, COL3A1 and FN1) were decreased in arteries treated with HuMoSCs or their supernatant. Likewise, concentrations of collagen-1 and VEGF were lower in the supernatants of TAs cultivated with HuMoSCs. In the presence of PDGF, the proliferation and migration of VSMCs were both decreased after treatment with HuMoSC supernatant. Study of the PDGF pathway suggests that HuMoSCs act through inhibition of mTOR activity. Finally, we show that HuMoSCs could be recruited in the arterial wall through the implication of CCR5 and its ligands.ConclusionAltogether, our results suggest that HuMoSCs or their supernatant could be useful to decrease vascular in flammation and remodeling in GCA, the latter being an unmet need in GCA treatment. suppressive cells giant cell arteritis treatment cellular therapy vascular remodeling Immunologic diseases. Allergy Maxime Samson verfasserin aut Maxime Samson verfasserin aut Coraline Genet verfasserin aut Marc Corbera-Bellalta verfasserin aut Hélène Greigert verfasserin aut Hélène Greigert verfasserin aut Georgina Espígol-Frigolé verfasserin aut Claire Gérard verfasserin aut Claudie Cladière verfasserin aut Roser Alba-Rovira verfasserin aut Marion Ciudad verfasserin aut Pierre-Henry Gabrielle verfasserin aut Catherine Creuzot-Garcher verfasserin aut Georges Tarris verfasserin aut Laurent Martin verfasserin aut Philippe Saas verfasserin aut Philippe Saas verfasserin aut Sylvain Audia verfasserin aut Sylvain Audia verfasserin aut Bernard Bonnotte verfasserin aut Bernard Bonnotte verfasserin aut Maria C. Cid verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 14(2023) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:14 year:2023 https://doi.org/10.3389/fimmu.2023.1137794 kostenfrei https://doaj.org/article/ad20cec6940f4fb28020e1bb5f5f6a43 kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2023.1137794/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023
allfieldsGer	10.3389/fimmu.2023.1137794 doi (DE-627)DOAJ086253271 (DE-599)DOAJad20cec6940f4fb28020e1bb5f5f6a43 DE-627 ger DE-627 rakwb eng RC581-607 Maxime Samson verfasserin aut Human monocyte-derived suppressive cells (HuMoSC) for cell therapy in giant cell arteritis 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier IntroductionThe pathogenesis of Giant Cell Arteritis (GCA) relies on vascular inflammation and vascular remodeling, the latter being poorly controlled by current treatments.MethodsThis study aimed to evaluate the effect of a novel cell therapy, Human Monocyte-derived Suppressor Cells (HuMoSC), on inflammation and vascular remodeling to improve GCA treatment. Fragments of temporal arteries (TAs) from GCA patients were cultured alone or in the presence of HuMoSCs or their supernatant. After five days, mRNA expression was measured in the TAs and proteins were measured in culture supernatant. The proliferation and migration capacity of vascular smooth muscle cells (VSMCs) were also analyzed with or without HuMoSC supernatant. ResultsTranscripts of genes implicated in vascular inflammation (CCL2, CCR2, CXCR3, HLADR), vascular remodeling (PDGF, PDGFR), angiogenesis (VEGF) and extracellular matrix composition (COL1A1, COL3A1 and FN1) were decreased in arteries treated with HuMoSCs or their supernatant. Likewise, concentrations of collagen-1 and VEGF were lower in the supernatants of TAs cultivated with HuMoSCs. In the presence of PDGF, the proliferation and migration of VSMCs were both decreased after treatment with HuMoSC supernatant. Study of the PDGF pathway suggests that HuMoSCs act through inhibition of mTOR activity. Finally, we show that HuMoSCs could be recruited in the arterial wall through the implication of CCR5 and its ligands.ConclusionAltogether, our results suggest that HuMoSCs or their supernatant could be useful to decrease vascular in flammation and remodeling in GCA, the latter being an unmet need in GCA treatment. suppressive cells giant cell arteritis treatment cellular therapy vascular remodeling Immunologic diseases. Allergy Maxime Samson verfasserin aut Maxime Samson verfasserin aut Coraline Genet verfasserin aut Marc Corbera-Bellalta verfasserin aut Hélène Greigert verfasserin aut Hélène Greigert verfasserin aut Georgina Espígol-Frigolé verfasserin aut Claire Gérard verfasserin aut Claudie Cladière verfasserin aut Roser Alba-Rovira verfasserin aut Marion Ciudad verfasserin aut Pierre-Henry Gabrielle verfasserin aut Catherine Creuzot-Garcher verfasserin aut Georges Tarris verfasserin aut Laurent Martin verfasserin aut Philippe Saas verfasserin aut Philippe Saas verfasserin aut Sylvain Audia verfasserin aut Sylvain Audia verfasserin aut Bernard Bonnotte verfasserin aut Bernard Bonnotte verfasserin aut Maria C. Cid verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 14(2023) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:14 year:2023 https://doi.org/10.3389/fimmu.2023.1137794 kostenfrei https://doaj.org/article/ad20cec6940f4fb28020e1bb5f5f6a43 kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2023.1137794/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023
allfieldsSound	10.3389/fimmu.2023.1137794 doi (DE-627)DOAJ086253271 (DE-599)DOAJad20cec6940f4fb28020e1bb5f5f6a43 DE-627 ger DE-627 rakwb eng RC581-607 Maxime Samson verfasserin aut Human monocyte-derived suppressive cells (HuMoSC) for cell therapy in giant cell arteritis 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier IntroductionThe pathogenesis of Giant Cell Arteritis (GCA) relies on vascular inflammation and vascular remodeling, the latter being poorly controlled by current treatments.MethodsThis study aimed to evaluate the effect of a novel cell therapy, Human Monocyte-derived Suppressor Cells (HuMoSC), on inflammation and vascular remodeling to improve GCA treatment. Fragments of temporal arteries (TAs) from GCA patients were cultured alone or in the presence of HuMoSCs or their supernatant. After five days, mRNA expression was measured in the TAs and proteins were measured in culture supernatant. The proliferation and migration capacity of vascular smooth muscle cells (VSMCs) were also analyzed with or without HuMoSC supernatant. ResultsTranscripts of genes implicated in vascular inflammation (CCL2, CCR2, CXCR3, HLADR), vascular remodeling (PDGF, PDGFR), angiogenesis (VEGF) and extracellular matrix composition (COL1A1, COL3A1 and FN1) were decreased in arteries treated with HuMoSCs or their supernatant. Likewise, concentrations of collagen-1 and VEGF were lower in the supernatants of TAs cultivated with HuMoSCs. In the presence of PDGF, the proliferation and migration of VSMCs were both decreased after treatment with HuMoSC supernatant. Study of the PDGF pathway suggests that HuMoSCs act through inhibition of mTOR activity. Finally, we show that HuMoSCs could be recruited in the arterial wall through the implication of CCR5 and its ligands.ConclusionAltogether, our results suggest that HuMoSCs or their supernatant could be useful to decrease vascular in flammation and remodeling in GCA, the latter being an unmet need in GCA treatment. suppressive cells giant cell arteritis treatment cellular therapy vascular remodeling Immunologic diseases. Allergy Maxime Samson verfasserin aut Maxime Samson verfasserin aut Coraline Genet verfasserin aut Marc Corbera-Bellalta verfasserin aut Hélène Greigert verfasserin aut Hélène Greigert verfasserin aut Georgina Espígol-Frigolé verfasserin aut Claire Gérard verfasserin aut Claudie Cladière verfasserin aut Roser Alba-Rovira verfasserin aut Marion Ciudad verfasserin aut Pierre-Henry Gabrielle verfasserin aut Catherine Creuzot-Garcher verfasserin aut Georges Tarris verfasserin aut Laurent Martin verfasserin aut Philippe Saas verfasserin aut Philippe Saas verfasserin aut Sylvain Audia verfasserin aut Sylvain Audia verfasserin aut Bernard Bonnotte verfasserin aut Bernard Bonnotte verfasserin aut Maria C. Cid verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 14(2023) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:14 year:2023 https://doi.org/10.3389/fimmu.2023.1137794 kostenfrei https://doaj.org/article/ad20cec6940f4fb28020e1bb5f5f6a43 kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2023.1137794/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023
language	English
source	In Frontiers in Immunology 14(2023) volume:14 year:2023
sourceStr	In Frontiers in Immunology 14(2023) volume:14 year:2023
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	suppressive cells giant cell arteritis treatment cellular therapy vascular remodeling Immunologic diseases. Allergy
isfreeaccess_bool	true
container_title	Frontiers in Immunology
authorswithroles_txt_mv	Maxime Samson @@aut@@ Coraline Genet @@aut@@ Marc Corbera-Bellalta @@aut@@ Hélène Greigert @@aut@@ Georgina Espígol-Frigolé @@aut@@ Claire Gérard @@aut@@ Claudie Cladière @@aut@@ Roser Alba-Rovira @@aut@@ Marion Ciudad @@aut@@ Pierre-Henry Gabrielle @@aut@@ Catherine Creuzot-Garcher @@aut@@ Georges Tarris @@aut@@ Laurent Martin @@aut@@ Philippe Saas @@aut@@ Sylvain Audia @@aut@@ Bernard Bonnotte @@aut@@ Maria C. Cid @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	657998354
id	DOAJ086253271
language_de	englisch
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callnumber-first	R - Medicine
author	Maxime Samson
spellingShingle	Maxime Samson misc RC581-607 misc suppressive cells misc giant cell arteritis misc treatment misc cellular therapy misc vascular remodeling misc Immunologic diseases. Allergy Human monocyte-derived suppressive cells (HuMoSC) for cell therapy in giant cell arteritis
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topic_title	RC581-607 Human monocyte-derived suppressive cells (HuMoSC) for cell therapy in giant cell arteritis suppressive cells giant cell arteritis treatment cellular therapy vascular remodeling
topic	misc RC581-607 misc suppressive cells misc giant cell arteritis misc treatment misc cellular therapy misc vascular remodeling misc Immunologic diseases. Allergy
topic_unstemmed	misc RC581-607 misc suppressive cells misc giant cell arteritis misc treatment misc cellular therapy misc vascular remodeling misc Immunologic diseases. Allergy
topic_browse	misc RC581-607 misc suppressive cells misc giant cell arteritis misc treatment misc cellular therapy misc vascular remodeling misc Immunologic diseases. Allergy
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title	Human monocyte-derived suppressive cells (HuMoSC) for cell therapy in giant cell arteritis
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title_full	Human monocyte-derived suppressive cells (HuMoSC) for cell therapy in giant cell arteritis
author_sort	Maxime Samson
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author_browse	Maxime Samson Coraline Genet Marc Corbera-Bellalta Hélène Greigert Georgina Espígol-Frigolé Claire Gérard Claudie Cladière Roser Alba-Rovira Marion Ciudad Pierre-Henry Gabrielle Catherine Creuzot-Garcher Georges Tarris Laurent Martin Philippe Saas Sylvain Audia Bernard Bonnotte Maria C. Cid
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doi_str_mv	10.3389/fimmu.2023.1137794
author2-role	verfasserin
title_sort	human monocyte-derived suppressive cells (humosc) for cell therapy in giant cell arteritis
callnumber	RC581-607
title_auth	Human monocyte-derived suppressive cells (HuMoSC) for cell therapy in giant cell arteritis
abstract	IntroductionThe pathogenesis of Giant Cell Arteritis (GCA) relies on vascular inflammation and vascular remodeling, the latter being poorly controlled by current treatments.MethodsThis study aimed to evaluate the effect of a novel cell therapy, Human Monocyte-derived Suppressor Cells (HuMoSC), on inflammation and vascular remodeling to improve GCA treatment. Fragments of temporal arteries (TAs) from GCA patients were cultured alone or in the presence of HuMoSCs or their supernatant. After five days, mRNA expression was measured in the TAs and proteins were measured in culture supernatant. The proliferation and migration capacity of vascular smooth muscle cells (VSMCs) were also analyzed with or without HuMoSC supernatant. ResultsTranscripts of genes implicated in vascular inflammation (CCL2, CCR2, CXCR3, HLADR), vascular remodeling (PDGF, PDGFR), angiogenesis (VEGF) and extracellular matrix composition (COL1A1, COL3A1 and FN1) were decreased in arteries treated with HuMoSCs or their supernatant. Likewise, concentrations of collagen-1 and VEGF were lower in the supernatants of TAs cultivated with HuMoSCs. In the presence of PDGF, the proliferation and migration of VSMCs were both decreased after treatment with HuMoSC supernatant. Study of the PDGF pathway suggests that HuMoSCs act through inhibition of mTOR activity. Finally, we show that HuMoSCs could be recruited in the arterial wall through the implication of CCR5 and its ligands.ConclusionAltogether, our results suggest that HuMoSCs or their supernatant could be useful to decrease vascular in flammation and remodeling in GCA, the latter being an unmet need in GCA treatment.
abstractGer	IntroductionThe pathogenesis of Giant Cell Arteritis (GCA) relies on vascular inflammation and vascular remodeling, the latter being poorly controlled by current treatments.MethodsThis study aimed to evaluate the effect of a novel cell therapy, Human Monocyte-derived Suppressor Cells (HuMoSC), on inflammation and vascular remodeling to improve GCA treatment. Fragments of temporal arteries (TAs) from GCA patients were cultured alone or in the presence of HuMoSCs or their supernatant. After five days, mRNA expression was measured in the TAs and proteins were measured in culture supernatant. The proliferation and migration capacity of vascular smooth muscle cells (VSMCs) were also analyzed with or without HuMoSC supernatant. ResultsTranscripts of genes implicated in vascular inflammation (CCL2, CCR2, CXCR3, HLADR), vascular remodeling (PDGF, PDGFR), angiogenesis (VEGF) and extracellular matrix composition (COL1A1, COL3A1 and FN1) were decreased in arteries treated with HuMoSCs or their supernatant. Likewise, concentrations of collagen-1 and VEGF were lower in the supernatants of TAs cultivated with HuMoSCs. In the presence of PDGF, the proliferation and migration of VSMCs were both decreased after treatment with HuMoSC supernatant. Study of the PDGF pathway suggests that HuMoSCs act through inhibition of mTOR activity. Finally, we show that HuMoSCs could be recruited in the arterial wall through the implication of CCR5 and its ligands.ConclusionAltogether, our results suggest that HuMoSCs or their supernatant could be useful to decrease vascular in flammation and remodeling in GCA, the latter being an unmet need in GCA treatment.
abstract_unstemmed	IntroductionThe pathogenesis of Giant Cell Arteritis (GCA) relies on vascular inflammation and vascular remodeling, the latter being poorly controlled by current treatments.MethodsThis study aimed to evaluate the effect of a novel cell therapy, Human Monocyte-derived Suppressor Cells (HuMoSC), on inflammation and vascular remodeling to improve GCA treatment. Fragments of temporal arteries (TAs) from GCA patients were cultured alone or in the presence of HuMoSCs or their supernatant. After five days, mRNA expression was measured in the TAs and proteins were measured in culture supernatant. The proliferation and migration capacity of vascular smooth muscle cells (VSMCs) were also analyzed with or without HuMoSC supernatant. ResultsTranscripts of genes implicated in vascular inflammation (CCL2, CCR2, CXCR3, HLADR), vascular remodeling (PDGF, PDGFR), angiogenesis (VEGF) and extracellular matrix composition (COL1A1, COL3A1 and FN1) were decreased in arteries treated with HuMoSCs or their supernatant. Likewise, concentrations of collagen-1 and VEGF were lower in the supernatants of TAs cultivated with HuMoSCs. In the presence of PDGF, the proliferation and migration of VSMCs were both decreased after treatment with HuMoSC supernatant. Study of the PDGF pathway suggests that HuMoSCs act through inhibition of mTOR activity. Finally, we show that HuMoSCs could be recruited in the arterial wall through the implication of CCR5 and its ligands.ConclusionAltogether, our results suggest that HuMoSCs or their supernatant could be useful to decrease vascular in flammation and remodeling in GCA, the latter being an unmet need in GCA treatment.
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title_short	Human monocyte-derived suppressive cells (HuMoSC) for cell therapy in giant cell arteritis
url	https://doi.org/10.3389/fimmu.2023.1137794 https://doaj.org/article/ad20cec6940f4fb28020e1bb5f5f6a43 https://www.frontiersin.org/articles/10.3389/fimmu.2023.1137794/full https://doaj.org/toc/1664-3224
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author2	Maxime Samson Coraline Genet Marc Corbera-Bellalta Hélène Greigert Georgina Espígol-Frigolé Claire Gérard Claudie Cladière Roser Alba-Rovira Marion Ciudad Pierre-Henry Gabrielle Catherine Creuzot-Garcher Georges Tarris Laurent Martin Philippe Saas Sylvain Audia Bernard Bonnotte Maria C. Cid
author2Str	Maxime Samson Coraline Genet Marc Corbera-Bellalta Hélène Greigert Georgina Espígol-Frigolé Claire Gérard Claudie Cladière Roser Alba-Rovira Marion Ciudad Pierre-Henry Gabrielle Catherine Creuzot-Garcher Georges Tarris Laurent Martin Philippe Saas Sylvain Audia Bernard Bonnotte Maria C. Cid
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up_date	2024-07-03T19:34:21.678Z
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Human monocyte-derived suppressive cells (HuMoSC) for cell therapy in giant cell arteritis

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