Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 <i<CYP4BQ1</i< Responsible for (+)-α-Pinene Tolerance in <i<Dendroctonus armandi</i<

Bark beetles mainly rely on detoxification enzymes to resist the host tree’s defense against oleoresin terpenes. Cytochrome P450 enzymes (CYPs) play an important role in the detoxification of plant allelochemicals and pesticides in insect. One P450 gene (<i<DaCYP4BQ1</i<) is associated w...
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Gespeichert in:

Autor*in:	Bin Liu [verfasserIn] Ming Tang [verfasserIn] Hui Chen [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	phytochemical tolerance cytochrome P450 oxidative burst CncC pathway

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 23(2022), 19, p 11578
Übergeordnetes Werk:	volume:23 ; year:2022 ; number:19, p 11578

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms231911578

Katalog-ID:	DOAJ086416286

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245	1	0	\|a Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 <i<CYP4BQ1</i< Responsible for (+)-α-Pinene Tolerance in <i<Dendroctonus armandi</i<
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520			\|a Bark beetles mainly rely on detoxification enzymes to resist the host tree’s defense against oleoresin terpenes. Cytochrome P450 enzymes (CYPs) play an important role in the detoxification of plant allelochemicals and pesticides in insect. One P450 gene (<i<DaCYP4BQ1</i<) is associated with the response of (+)-α-pinene in <i<Dendroctonus armandi</i<. However, the regulatory mechanism of this P450 gene response to (+)-α-pinene is still unknown. In this study, spatiotemporal expression profiling indicated that <i<CYP4BQ1</i< was highly expressed in adult and larval stages of <i<D. armandi</i<, and it was predominantly expressed in fat body, midgut, and Malpighian tubules of adults. Moreover, the expression of <i<CYP4BQ1</i< significantly increased after exposure to (+)-α-pinene, and depletion of it decreased the tolerance of adults to (+)-α-pinene. In addition, (+)-α-pinene treatment induced the expression of the transcription factors cap ‘n’ collar isoform C (<i<CncC</i<) and its binding factor muscle aponeurosis fibromatosis (<i<Maf</i<), elevated the level of hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<), and increased the activities of antioxidant enzymes. Silencing <i<CncC</i< suppressed <i<CYP4BQ1</i< expression and enhanced the susceptibility of beetles to (+)-α-pinene. Similarly, application of the reactive oxygen species (ROS) scavenger N-acetylcysteine reduced the production and accumulation of H<sub<2</sub<O<sub<2</sub<, suppressed the expression of <i<CncC, Maf</i<, and <i<CYP4BQ1</i< and led to decreased tolerance of adults to (+)-α-pinene. In contrast, ingestion of the CncC agonist curcumin elevated <i<CYP4BQ1</i< expression and enhanced (+)-α-pinene tolerance. The results demonstrate that, in <i<D. armandi</i<, (+)-α-pinene induces <i<CYP4BQ1</i< via activation of the ROS/CncC signaling pathway.
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allfields	10.3390/ijms231911578 doi (DE-627)DOAJ086416286 (DE-599)DOAJ7aac8485bfed4e999f6e767048c44376 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Bin Liu verfasserin aut Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 <i<CYP4BQ1</i< Responsible for (+)-α-Pinene Tolerance in <i<Dendroctonus armandi</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Bark beetles mainly rely on detoxification enzymes to resist the host tree’s defense against oleoresin terpenes. Cytochrome P450 enzymes (CYPs) play an important role in the detoxification of plant allelochemicals and pesticides in insect. One P450 gene (<i<DaCYP4BQ1</i<) is associated with the response of (+)-α-pinene in <i<Dendroctonus armandi</i<. However, the regulatory mechanism of this P450 gene response to (+)-α-pinene is still unknown. In this study, spatiotemporal expression profiling indicated that <i<CYP4BQ1</i< was highly expressed in adult and larval stages of <i<D. armandi</i<, and it was predominantly expressed in fat body, midgut, and Malpighian tubules of adults. Moreover, the expression of <i<CYP4BQ1</i< significantly increased after exposure to (+)-α-pinene, and depletion of it decreased the tolerance of adults to (+)-α-pinene. In addition, (+)-α-pinene treatment induced the expression of the transcription factors cap ‘n’ collar isoform C (<i<CncC</i<) and its binding factor muscle aponeurosis fibromatosis (<i<Maf</i<), elevated the level of hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<), and increased the activities of antioxidant enzymes. Silencing <i<CncC</i< suppressed <i<CYP4BQ1</i< expression and enhanced the susceptibility of beetles to (+)-α-pinene. Similarly, application of the reactive oxygen species (ROS) scavenger N-acetylcysteine reduced the production and accumulation of H<sub<2</sub<O<sub<2</sub<, suppressed the expression of <i<CncC, Maf</i<, and <i<CYP4BQ1</i< and led to decreased tolerance of adults to (+)-α-pinene. In contrast, ingestion of the CncC agonist curcumin elevated <i<CYP4BQ1</i< expression and enhanced (+)-α-pinene tolerance. The results demonstrate that, in <i<D. armandi</i<, (+)-α-pinene induces <i<CYP4BQ1</i< via activation of the ROS/CncC signaling pathway. <i<Dendroctonus armandi</i< phytochemical tolerance cytochrome P450 oxidative burst CncC pathway Biology (General) Chemistry Ming Tang verfasserin aut Hui Chen verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 19, p 11578 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:19, p 11578 https://doi.org/10.3390/ijms231911578 kostenfrei https://doaj.org/article/7aac8485bfed4e999f6e767048c44376 kostenfrei https://www.mdpi.com/1422-0067/23/19/11578 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 19, p 11578
spelling	10.3390/ijms231911578 doi (DE-627)DOAJ086416286 (DE-599)DOAJ7aac8485bfed4e999f6e767048c44376 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Bin Liu verfasserin aut Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 <i<CYP4BQ1</i< Responsible for (+)-α-Pinene Tolerance in <i<Dendroctonus armandi</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Bark beetles mainly rely on detoxification enzymes to resist the host tree’s defense against oleoresin terpenes. Cytochrome P450 enzymes (CYPs) play an important role in the detoxification of plant allelochemicals and pesticides in insect. One P450 gene (<i<DaCYP4BQ1</i<) is associated with the response of (+)-α-pinene in <i<Dendroctonus armandi</i<. However, the regulatory mechanism of this P450 gene response to (+)-α-pinene is still unknown. In this study, spatiotemporal expression profiling indicated that <i<CYP4BQ1</i< was highly expressed in adult and larval stages of <i<D. armandi</i<, and it was predominantly expressed in fat body, midgut, and Malpighian tubules of adults. Moreover, the expression of <i<CYP4BQ1</i< significantly increased after exposure to (+)-α-pinene, and depletion of it decreased the tolerance of adults to (+)-α-pinene. In addition, (+)-α-pinene treatment induced the expression of the transcription factors cap ‘n’ collar isoform C (<i<CncC</i<) and its binding factor muscle aponeurosis fibromatosis (<i<Maf</i<), elevated the level of hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<), and increased the activities of antioxidant enzymes. Silencing <i<CncC</i< suppressed <i<CYP4BQ1</i< expression and enhanced the susceptibility of beetles to (+)-α-pinene. Similarly, application of the reactive oxygen species (ROS) scavenger N-acetylcysteine reduced the production and accumulation of H<sub<2</sub<O<sub<2</sub<, suppressed the expression of <i<CncC, Maf</i<, and <i<CYP4BQ1</i< and led to decreased tolerance of adults to (+)-α-pinene. In contrast, ingestion of the CncC agonist curcumin elevated <i<CYP4BQ1</i< expression and enhanced (+)-α-pinene tolerance. The results demonstrate that, in <i<D. armandi</i<, (+)-α-pinene induces <i<CYP4BQ1</i< via activation of the ROS/CncC signaling pathway. <i<Dendroctonus armandi</i< phytochemical tolerance cytochrome P450 oxidative burst CncC pathway Biology (General) Chemistry Ming Tang verfasserin aut Hui Chen verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 19, p 11578 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:19, p 11578 https://doi.org/10.3390/ijms231911578 kostenfrei https://doaj.org/article/7aac8485bfed4e999f6e767048c44376 kostenfrei https://www.mdpi.com/1422-0067/23/19/11578 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 19, p 11578
allfields_unstemmed	10.3390/ijms231911578 doi (DE-627)DOAJ086416286 (DE-599)DOAJ7aac8485bfed4e999f6e767048c44376 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Bin Liu verfasserin aut Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 <i<CYP4BQ1</i< Responsible for (+)-α-Pinene Tolerance in <i<Dendroctonus armandi</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Bark beetles mainly rely on detoxification enzymes to resist the host tree’s defense against oleoresin terpenes. Cytochrome P450 enzymes (CYPs) play an important role in the detoxification of plant allelochemicals and pesticides in insect. One P450 gene (<i<DaCYP4BQ1</i<) is associated with the response of (+)-α-pinene in <i<Dendroctonus armandi</i<. However, the regulatory mechanism of this P450 gene response to (+)-α-pinene is still unknown. In this study, spatiotemporal expression profiling indicated that <i<CYP4BQ1</i< was highly expressed in adult and larval stages of <i<D. armandi</i<, and it was predominantly expressed in fat body, midgut, and Malpighian tubules of adults. Moreover, the expression of <i<CYP4BQ1</i< significantly increased after exposure to (+)-α-pinene, and depletion of it decreased the tolerance of adults to (+)-α-pinene. In addition, (+)-α-pinene treatment induced the expression of the transcription factors cap ‘n’ collar isoform C (<i<CncC</i<) and its binding factor muscle aponeurosis fibromatosis (<i<Maf</i<), elevated the level of hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<), and increased the activities of antioxidant enzymes. Silencing <i<CncC</i< suppressed <i<CYP4BQ1</i< expression and enhanced the susceptibility of beetles to (+)-α-pinene. Similarly, application of the reactive oxygen species (ROS) scavenger N-acetylcysteine reduced the production and accumulation of H<sub<2</sub<O<sub<2</sub<, suppressed the expression of <i<CncC, Maf</i<, and <i<CYP4BQ1</i< and led to decreased tolerance of adults to (+)-α-pinene. In contrast, ingestion of the CncC agonist curcumin elevated <i<CYP4BQ1</i< expression and enhanced (+)-α-pinene tolerance. The results demonstrate that, in <i<D. armandi</i<, (+)-α-pinene induces <i<CYP4BQ1</i< via activation of the ROS/CncC signaling pathway. <i<Dendroctonus armandi</i< phytochemical tolerance cytochrome P450 oxidative burst CncC pathway Biology (General) Chemistry Ming Tang verfasserin aut Hui Chen verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 19, p 11578 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:19, p 11578 https://doi.org/10.3390/ijms231911578 kostenfrei https://doaj.org/article/7aac8485bfed4e999f6e767048c44376 kostenfrei https://www.mdpi.com/1422-0067/23/19/11578 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 19, p 11578
allfieldsGer	10.3390/ijms231911578 doi (DE-627)DOAJ086416286 (DE-599)DOAJ7aac8485bfed4e999f6e767048c44376 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Bin Liu verfasserin aut Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 <i<CYP4BQ1</i< Responsible for (+)-α-Pinene Tolerance in <i<Dendroctonus armandi</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Bark beetles mainly rely on detoxification enzymes to resist the host tree’s defense against oleoresin terpenes. Cytochrome P450 enzymes (CYPs) play an important role in the detoxification of plant allelochemicals and pesticides in insect. One P450 gene (<i<DaCYP4BQ1</i<) is associated with the response of (+)-α-pinene in <i<Dendroctonus armandi</i<. However, the regulatory mechanism of this P450 gene response to (+)-α-pinene is still unknown. In this study, spatiotemporal expression profiling indicated that <i<CYP4BQ1</i< was highly expressed in adult and larval stages of <i<D. armandi</i<, and it was predominantly expressed in fat body, midgut, and Malpighian tubules of adults. Moreover, the expression of <i<CYP4BQ1</i< significantly increased after exposure to (+)-α-pinene, and depletion of it decreased the tolerance of adults to (+)-α-pinene. In addition, (+)-α-pinene treatment induced the expression of the transcription factors cap ‘n’ collar isoform C (<i<CncC</i<) and its binding factor muscle aponeurosis fibromatosis (<i<Maf</i<), elevated the level of hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<), and increased the activities of antioxidant enzymes. Silencing <i<CncC</i< suppressed <i<CYP4BQ1</i< expression and enhanced the susceptibility of beetles to (+)-α-pinene. Similarly, application of the reactive oxygen species (ROS) scavenger N-acetylcysteine reduced the production and accumulation of H<sub<2</sub<O<sub<2</sub<, suppressed the expression of <i<CncC, Maf</i<, and <i<CYP4BQ1</i< and led to decreased tolerance of adults to (+)-α-pinene. In contrast, ingestion of the CncC agonist curcumin elevated <i<CYP4BQ1</i< expression and enhanced (+)-α-pinene tolerance. The results demonstrate that, in <i<D. armandi</i<, (+)-α-pinene induces <i<CYP4BQ1</i< via activation of the ROS/CncC signaling pathway. <i<Dendroctonus armandi</i< phytochemical tolerance cytochrome P450 oxidative burst CncC pathway Biology (General) Chemistry Ming Tang verfasserin aut Hui Chen verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 19, p 11578 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:19, p 11578 https://doi.org/10.3390/ijms231911578 kostenfrei https://doaj.org/article/7aac8485bfed4e999f6e767048c44376 kostenfrei https://www.mdpi.com/1422-0067/23/19/11578 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 19, p 11578
allfieldsSound	10.3390/ijms231911578 doi (DE-627)DOAJ086416286 (DE-599)DOAJ7aac8485bfed4e999f6e767048c44376 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Bin Liu verfasserin aut Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 <i<CYP4BQ1</i< Responsible for (+)-α-Pinene Tolerance in <i<Dendroctonus armandi</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Bark beetles mainly rely on detoxification enzymes to resist the host tree’s defense against oleoresin terpenes. Cytochrome P450 enzymes (CYPs) play an important role in the detoxification of plant allelochemicals and pesticides in insect. One P450 gene (<i<DaCYP4BQ1</i<) is associated with the response of (+)-α-pinene in <i<Dendroctonus armandi</i<. However, the regulatory mechanism of this P450 gene response to (+)-α-pinene is still unknown. In this study, spatiotemporal expression profiling indicated that <i<CYP4BQ1</i< was highly expressed in adult and larval stages of <i<D. armandi</i<, and it was predominantly expressed in fat body, midgut, and Malpighian tubules of adults. Moreover, the expression of <i<CYP4BQ1</i< significantly increased after exposure to (+)-α-pinene, and depletion of it decreased the tolerance of adults to (+)-α-pinene. In addition, (+)-α-pinene treatment induced the expression of the transcription factors cap ‘n’ collar isoform C (<i<CncC</i<) and its binding factor muscle aponeurosis fibromatosis (<i<Maf</i<), elevated the level of hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<), and increased the activities of antioxidant enzymes. Silencing <i<CncC</i< suppressed <i<CYP4BQ1</i< expression and enhanced the susceptibility of beetles to (+)-α-pinene. Similarly, application of the reactive oxygen species (ROS) scavenger N-acetylcysteine reduced the production and accumulation of H<sub<2</sub<O<sub<2</sub<, suppressed the expression of <i<CncC, Maf</i<, and <i<CYP4BQ1</i< and led to decreased tolerance of adults to (+)-α-pinene. In contrast, ingestion of the CncC agonist curcumin elevated <i<CYP4BQ1</i< expression and enhanced (+)-α-pinene tolerance. The results demonstrate that, in <i<D. armandi</i<, (+)-α-pinene induces <i<CYP4BQ1</i< via activation of the ROS/CncC signaling pathway. <i<Dendroctonus armandi</i< phytochemical tolerance cytochrome P450 oxidative burst CncC pathway Biology (General) Chemistry Ming Tang verfasserin aut Hui Chen verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 19, p 11578 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:19, p 11578 https://doi.org/10.3390/ijms231911578 kostenfrei https://doaj.org/article/7aac8485bfed4e999f6e767048c44376 kostenfrei https://www.mdpi.com/1422-0067/23/19/11578 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 19, p 11578
language	English
source	In International Journal of Molecular Sciences 23(2022), 19, p 11578 volume:23 year:2022 number:19, p 11578
sourceStr	In International Journal of Molecular Sciences 23(2022), 19, p 11578 volume:23 year:2022 number:19, p 11578
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<Dendroctonus armandi</i< phytochemical tolerance cytochrome P450 oxidative burst CncC pathway Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Bin Liu @@aut@@ Ming Tang @@aut@@ Hui Chen @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ086416286
language_de	englisch
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callnumber-first	Q - Science
author	Bin Liu
spellingShingle	Bin Liu misc QH301-705.5 misc QD1-999 misc <i<Dendroctonus armandi</i< misc phytochemical tolerance misc cytochrome P450 misc oxidative burst misc CncC pathway misc Biology (General) misc Chemistry Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 <i<CYP4BQ1</i< Responsible for (+)-α-Pinene Tolerance in <i<Dendroctonus armandi</i<
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topic_title	QH301-705.5 QD1-999 Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 <i<CYP4BQ1</i< Responsible for (+)-α-Pinene Tolerance in <i<Dendroctonus armandi</i< <i<Dendroctonus armandi</i< phytochemical tolerance cytochrome P450 oxidative burst CncC pathway
topic	misc QH301-705.5 misc QD1-999 misc <i<Dendroctonus armandi</i< misc phytochemical tolerance misc cytochrome P450 misc oxidative burst misc CncC pathway misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc <i<Dendroctonus armandi</i< misc phytochemical tolerance misc cytochrome P450 misc oxidative burst misc CncC pathway misc Biology (General) misc Chemistry
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title	Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 <i<CYP4BQ1</i< Responsible for (+)-α-Pinene Tolerance in <i<Dendroctonus armandi</i<
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title_full	Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 <i<CYP4BQ1</i< Responsible for (+)-α-Pinene Tolerance in <i<Dendroctonus armandi</i<
author_sort	Bin Liu
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author_browse	Bin Liu Ming Tang Hui Chen
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class	QH301-705.5 QD1-999
format_se	Elektronische Aufsätze
author-letter	Bin Liu
doi_str_mv	10.3390/ijms231911578
author2-role	verfasserin
title_sort	activation of the ros/cncc signaling pathway regulates cytochrome p450 <i<cyp4bq1</i< responsible for (+)-α-pinene tolerance in <i<dendroctonus armandi</i<
callnumber	QH301-705.5
title_auth	Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 <i<CYP4BQ1</i< Responsible for (+)-α-Pinene Tolerance in <i<Dendroctonus armandi</i<
abstract	Bark beetles mainly rely on detoxification enzymes to resist the host tree’s defense against oleoresin terpenes. Cytochrome P450 enzymes (CYPs) play an important role in the detoxification of plant allelochemicals and pesticides in insect. One P450 gene (<i<DaCYP4BQ1</i<) is associated with the response of (+)-α-pinene in <i<Dendroctonus armandi</i<. However, the regulatory mechanism of this P450 gene response to (+)-α-pinene is still unknown. In this study, spatiotemporal expression profiling indicated that <i<CYP4BQ1</i< was highly expressed in adult and larval stages of <i<D. armandi</i<, and it was predominantly expressed in fat body, midgut, and Malpighian tubules of adults. Moreover, the expression of <i<CYP4BQ1</i< significantly increased after exposure to (+)-α-pinene, and depletion of it decreased the tolerance of adults to (+)-α-pinene. In addition, (+)-α-pinene treatment induced the expression of the transcription factors cap ‘n’ collar isoform C (<i<CncC</i<) and its binding factor muscle aponeurosis fibromatosis (<i<Maf</i<), elevated the level of hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<), and increased the activities of antioxidant enzymes. Silencing <i<CncC</i< suppressed <i<CYP4BQ1</i< expression and enhanced the susceptibility of beetles to (+)-α-pinene. Similarly, application of the reactive oxygen species (ROS) scavenger N-acetylcysteine reduced the production and accumulation of H<sub<2</sub<O<sub<2</sub<, suppressed the expression of <i<CncC, Maf</i<, and <i<CYP4BQ1</i< and led to decreased tolerance of adults to (+)-α-pinene. In contrast, ingestion of the CncC agonist curcumin elevated <i<CYP4BQ1</i< expression and enhanced (+)-α-pinene tolerance. The results demonstrate that, in <i<D. armandi</i<, (+)-α-pinene induces <i<CYP4BQ1</i< via activation of the ROS/CncC signaling pathway.
abstractGer	Bark beetles mainly rely on detoxification enzymes to resist the host tree’s defense against oleoresin terpenes. Cytochrome P450 enzymes (CYPs) play an important role in the detoxification of plant allelochemicals and pesticides in insect. One P450 gene (<i<DaCYP4BQ1</i<) is associated with the response of (+)-α-pinene in <i<Dendroctonus armandi</i<. However, the regulatory mechanism of this P450 gene response to (+)-α-pinene is still unknown. In this study, spatiotemporal expression profiling indicated that <i<CYP4BQ1</i< was highly expressed in adult and larval stages of <i<D. armandi</i<, and it was predominantly expressed in fat body, midgut, and Malpighian tubules of adults. Moreover, the expression of <i<CYP4BQ1</i< significantly increased after exposure to (+)-α-pinene, and depletion of it decreased the tolerance of adults to (+)-α-pinene. In addition, (+)-α-pinene treatment induced the expression of the transcription factors cap ‘n’ collar isoform C (<i<CncC</i<) and its binding factor muscle aponeurosis fibromatosis (<i<Maf</i<), elevated the level of hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<), and increased the activities of antioxidant enzymes. Silencing <i<CncC</i< suppressed <i<CYP4BQ1</i< expression and enhanced the susceptibility of beetles to (+)-α-pinene. Similarly, application of the reactive oxygen species (ROS) scavenger N-acetylcysteine reduced the production and accumulation of H<sub<2</sub<O<sub<2</sub<, suppressed the expression of <i<CncC, Maf</i<, and <i<CYP4BQ1</i< and led to decreased tolerance of adults to (+)-α-pinene. In contrast, ingestion of the CncC agonist curcumin elevated <i<CYP4BQ1</i< expression and enhanced (+)-α-pinene tolerance. The results demonstrate that, in <i<D. armandi</i<, (+)-α-pinene induces <i<CYP4BQ1</i< via activation of the ROS/CncC signaling pathway.
abstract_unstemmed	Bark beetles mainly rely on detoxification enzymes to resist the host tree’s defense against oleoresin terpenes. Cytochrome P450 enzymes (CYPs) play an important role in the detoxification of plant allelochemicals and pesticides in insect. One P450 gene (<i<DaCYP4BQ1</i<) is associated with the response of (+)-α-pinene in <i<Dendroctonus armandi</i<. However, the regulatory mechanism of this P450 gene response to (+)-α-pinene is still unknown. In this study, spatiotemporal expression profiling indicated that <i<CYP4BQ1</i< was highly expressed in adult and larval stages of <i<D. armandi</i<, and it was predominantly expressed in fat body, midgut, and Malpighian tubules of adults. Moreover, the expression of <i<CYP4BQ1</i< significantly increased after exposure to (+)-α-pinene, and depletion of it decreased the tolerance of adults to (+)-α-pinene. In addition, (+)-α-pinene treatment induced the expression of the transcription factors cap ‘n’ collar isoform C (<i<CncC</i<) and its binding factor muscle aponeurosis fibromatosis (<i<Maf</i<), elevated the level of hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<), and increased the activities of antioxidant enzymes. Silencing <i<CncC</i< suppressed <i<CYP4BQ1</i< expression and enhanced the susceptibility of beetles to (+)-α-pinene. Similarly, application of the reactive oxygen species (ROS) scavenger N-acetylcysteine reduced the production and accumulation of H<sub<2</sub<O<sub<2</sub<, suppressed the expression of <i<CncC, Maf</i<, and <i<CYP4BQ1</i< and led to decreased tolerance of adults to (+)-α-pinene. In contrast, ingestion of the CncC agonist curcumin elevated <i<CYP4BQ1</i< expression and enhanced (+)-α-pinene tolerance. The results demonstrate that, in <i<D. armandi</i<, (+)-α-pinene induces <i<CYP4BQ1</i< via activation of the ROS/CncC signaling pathway.
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container_issue	19, p 11578
title_short	Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 <i<CYP4BQ1</i< Responsible for (+)-α-Pinene Tolerance in <i<Dendroctonus armandi</i<
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Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 <i<CYP4BQ1</i< Responsible for (+)-α-Pinene Tolerance in <i<Dendroctonus armandi</i<

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