Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory Immunity
Beneficial microbes with immunomodulatory capacities (immunobiotics) and their non-viable forms (postimmunobiotics) could be effectively utilized in formulations towards the prevention of respiratory viral infections. In this study, novel immunobiotic strains with the ability to increase antiviral i...
Ausführliche Beschreibung
Autor*in: |
Mikado Tomokiyo [verfasserIn] Fernanda Raya Tonetti [verfasserIn] Hikari Yamamuro [verfasserIn] Ryoko Shibata [verfasserIn] Kohtaro Fukuyama [verfasserIn] Nadia Gobbato [verfasserIn] Leonardo Albarracin [verfasserIn] Muhammad Shahid Riaz Rajoka [verfasserIn] A. K. M. Humayun Kober [verfasserIn] Wakako Ikeda-Ohtsubo [verfasserIn] Julio Villena [verfasserIn] Haruki Kitazawa [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2022 |
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Schlagwörter: |
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Übergeordnetes Werk: |
In: Cells - MDPI AG, 2012, 11(2022), 19, p 2986 |
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Übergeordnetes Werk: |
volume:11 ; year:2022 ; number:19, p 2986 |
Links: |
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DOI / URN: |
10.3390/cells11192986 |
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Katalog-ID: |
DOAJ086427091 |
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10.3390/cells11192986 doi (DE-627)DOAJ086427091 (DE-599)DOAJ2ff7a67699f94eb3bc1fc79eac1c0470 DE-627 ger DE-627 rakwb eng QH573-671 Mikado Tomokiyo verfasserin aut Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory Immunity 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Beneficial microbes with immunomodulatory capacities (immunobiotics) and their non-viable forms (postimmunobiotics) could be effectively utilized in formulations towards the prevention of respiratory viral infections. In this study, novel immunobiotic strains with the ability to increase antiviral immunity in porcine alveolar macrophages were selected from a library of <i<Lactobacillus gasseri</i<. Postimmunobiotics derived from the most remarkable strains were also evaluated in their capacity to modulate the immune response triggered by Toll-like receptor 3 (TLR3) in alveolar macrophages and to differentially regulate TLR3-mediated antiviral respiratory immunity in infant mice. We provide evidence that porcine alveolar macrophages (3D4/31 cells) are a useful in vitro tool for the screening of new antiviral immunobiotics and postimmunobiotics by assessing their ability to modulate the expression <i<IFN-β</i<, <i<IFN-λ1</i<, <i<RNAseL</i<, <i<Mx2</i<, and <i<IL-6</i<, which can be used as prospective biomarkers. We also demonstrate that the postimmunobiotics derived from the <i<Lactobacillus gasseri</i< TMT36, TMT39 and TMT40 (HK36, HK39 or HK40) strains modulate the innate antiviral immune response of alveolar macrophages and reduce lung inflammatory damage triggered by TLR3 activation in vivo. Although our findings should be deepened and expanded, the results of the present work provide a scientific rationale for the use of nasally administered HK36, HK39 or HK40 to beneficially modulate TLR3-triggerd respiratory innate immune response. porcine alveolar macrophages TLR3 immunobiotics postimmunobiotics <i<Lactobacillus gasseri</i< lung inflammatory damage Cytology Fernanda Raya Tonetti verfasserin aut Hikari Yamamuro verfasserin aut Ryoko Shibata verfasserin aut Kohtaro Fukuyama verfasserin aut Nadia Gobbato verfasserin aut Leonardo Albarracin verfasserin aut Muhammad Shahid Riaz Rajoka verfasserin aut A. K. M. Humayun Kober verfasserin aut Wakako Ikeda-Ohtsubo verfasserin aut Julio Villena verfasserin aut Haruki Kitazawa verfasserin aut In Cells MDPI AG, 2012 11(2022), 19, p 2986 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:11 year:2022 number:19, p 2986 https://doi.org/10.3390/cells11192986 kostenfrei https://doaj.org/article/2ff7a67699f94eb3bc1fc79eac1c0470 kostenfrei https://www.mdpi.com/2073-4409/11/19/2986 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 19, p 2986 |
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10.3390/cells11192986 doi (DE-627)DOAJ086427091 (DE-599)DOAJ2ff7a67699f94eb3bc1fc79eac1c0470 DE-627 ger DE-627 rakwb eng QH573-671 Mikado Tomokiyo verfasserin aut Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory Immunity 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Beneficial microbes with immunomodulatory capacities (immunobiotics) and their non-viable forms (postimmunobiotics) could be effectively utilized in formulations towards the prevention of respiratory viral infections. In this study, novel immunobiotic strains with the ability to increase antiviral immunity in porcine alveolar macrophages were selected from a library of <i<Lactobacillus gasseri</i<. Postimmunobiotics derived from the most remarkable strains were also evaluated in their capacity to modulate the immune response triggered by Toll-like receptor 3 (TLR3) in alveolar macrophages and to differentially regulate TLR3-mediated antiviral respiratory immunity in infant mice. We provide evidence that porcine alveolar macrophages (3D4/31 cells) are a useful in vitro tool for the screening of new antiviral immunobiotics and postimmunobiotics by assessing their ability to modulate the expression <i<IFN-β</i<, <i<IFN-λ1</i<, <i<RNAseL</i<, <i<Mx2</i<, and <i<IL-6</i<, which can be used as prospective biomarkers. We also demonstrate that the postimmunobiotics derived from the <i<Lactobacillus gasseri</i< TMT36, TMT39 and TMT40 (HK36, HK39 or HK40) strains modulate the innate antiviral immune response of alveolar macrophages and reduce lung inflammatory damage triggered by TLR3 activation in vivo. Although our findings should be deepened and expanded, the results of the present work provide a scientific rationale for the use of nasally administered HK36, HK39 or HK40 to beneficially modulate TLR3-triggerd respiratory innate immune response. porcine alveolar macrophages TLR3 immunobiotics postimmunobiotics <i<Lactobacillus gasseri</i< lung inflammatory damage Cytology Fernanda Raya Tonetti verfasserin aut Hikari Yamamuro verfasserin aut Ryoko Shibata verfasserin aut Kohtaro Fukuyama verfasserin aut Nadia Gobbato verfasserin aut Leonardo Albarracin verfasserin aut Muhammad Shahid Riaz Rajoka verfasserin aut A. K. M. Humayun Kober verfasserin aut Wakako Ikeda-Ohtsubo verfasserin aut Julio Villena verfasserin aut Haruki Kitazawa verfasserin aut In Cells MDPI AG, 2012 11(2022), 19, p 2986 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:11 year:2022 number:19, p 2986 https://doi.org/10.3390/cells11192986 kostenfrei https://doaj.org/article/2ff7a67699f94eb3bc1fc79eac1c0470 kostenfrei https://www.mdpi.com/2073-4409/11/19/2986 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 19, p 2986 |
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10.3390/cells11192986 doi (DE-627)DOAJ086427091 (DE-599)DOAJ2ff7a67699f94eb3bc1fc79eac1c0470 DE-627 ger DE-627 rakwb eng QH573-671 Mikado Tomokiyo verfasserin aut Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory Immunity 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Beneficial microbes with immunomodulatory capacities (immunobiotics) and their non-viable forms (postimmunobiotics) could be effectively utilized in formulations towards the prevention of respiratory viral infections. In this study, novel immunobiotic strains with the ability to increase antiviral immunity in porcine alveolar macrophages were selected from a library of <i<Lactobacillus gasseri</i<. Postimmunobiotics derived from the most remarkable strains were also evaluated in their capacity to modulate the immune response triggered by Toll-like receptor 3 (TLR3) in alveolar macrophages and to differentially regulate TLR3-mediated antiviral respiratory immunity in infant mice. We provide evidence that porcine alveolar macrophages (3D4/31 cells) are a useful in vitro tool for the screening of new antiviral immunobiotics and postimmunobiotics by assessing their ability to modulate the expression <i<IFN-β</i<, <i<IFN-λ1</i<, <i<RNAseL</i<, <i<Mx2</i<, and <i<IL-6</i<, which can be used as prospective biomarkers. We also demonstrate that the postimmunobiotics derived from the <i<Lactobacillus gasseri</i< TMT36, TMT39 and TMT40 (HK36, HK39 or HK40) strains modulate the innate antiviral immune response of alveolar macrophages and reduce lung inflammatory damage triggered by TLR3 activation in vivo. Although our findings should be deepened and expanded, the results of the present work provide a scientific rationale for the use of nasally administered HK36, HK39 or HK40 to beneficially modulate TLR3-triggerd respiratory innate immune response. porcine alveolar macrophages TLR3 immunobiotics postimmunobiotics <i<Lactobacillus gasseri</i< lung inflammatory damage Cytology Fernanda Raya Tonetti verfasserin aut Hikari Yamamuro verfasserin aut Ryoko Shibata verfasserin aut Kohtaro Fukuyama verfasserin aut Nadia Gobbato verfasserin aut Leonardo Albarracin verfasserin aut Muhammad Shahid Riaz Rajoka verfasserin aut A. K. M. Humayun Kober verfasserin aut Wakako Ikeda-Ohtsubo verfasserin aut Julio Villena verfasserin aut Haruki Kitazawa verfasserin aut In Cells MDPI AG, 2012 11(2022), 19, p 2986 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:11 year:2022 number:19, p 2986 https://doi.org/10.3390/cells11192986 kostenfrei https://doaj.org/article/2ff7a67699f94eb3bc1fc79eac1c0470 kostenfrei https://www.mdpi.com/2073-4409/11/19/2986 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 19, p 2986 |
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Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory Immunity |
abstract |
Beneficial microbes with immunomodulatory capacities (immunobiotics) and their non-viable forms (postimmunobiotics) could be effectively utilized in formulations towards the prevention of respiratory viral infections. In this study, novel immunobiotic strains with the ability to increase antiviral immunity in porcine alveolar macrophages were selected from a library of <i<Lactobacillus gasseri</i<. Postimmunobiotics derived from the most remarkable strains were also evaluated in their capacity to modulate the immune response triggered by Toll-like receptor 3 (TLR3) in alveolar macrophages and to differentially regulate TLR3-mediated antiviral respiratory immunity in infant mice. We provide evidence that porcine alveolar macrophages (3D4/31 cells) are a useful in vitro tool for the screening of new antiviral immunobiotics and postimmunobiotics by assessing their ability to modulate the expression <i<IFN-β</i<, <i<IFN-λ1</i<, <i<RNAseL</i<, <i<Mx2</i<, and <i<IL-6</i<, which can be used as prospective biomarkers. We also demonstrate that the postimmunobiotics derived from the <i<Lactobacillus gasseri</i< TMT36, TMT39 and TMT40 (HK36, HK39 or HK40) strains modulate the innate antiviral immune response of alveolar macrophages and reduce lung inflammatory damage triggered by TLR3 activation in vivo. Although our findings should be deepened and expanded, the results of the present work provide a scientific rationale for the use of nasally administered HK36, HK39 or HK40 to beneficially modulate TLR3-triggerd respiratory innate immune response. |
abstractGer |
Beneficial microbes with immunomodulatory capacities (immunobiotics) and their non-viable forms (postimmunobiotics) could be effectively utilized in formulations towards the prevention of respiratory viral infections. In this study, novel immunobiotic strains with the ability to increase antiviral immunity in porcine alveolar macrophages were selected from a library of <i<Lactobacillus gasseri</i<. Postimmunobiotics derived from the most remarkable strains were also evaluated in their capacity to modulate the immune response triggered by Toll-like receptor 3 (TLR3) in alveolar macrophages and to differentially regulate TLR3-mediated antiviral respiratory immunity in infant mice. We provide evidence that porcine alveolar macrophages (3D4/31 cells) are a useful in vitro tool for the screening of new antiviral immunobiotics and postimmunobiotics by assessing their ability to modulate the expression <i<IFN-β</i<, <i<IFN-λ1</i<, <i<RNAseL</i<, <i<Mx2</i<, and <i<IL-6</i<, which can be used as prospective biomarkers. We also demonstrate that the postimmunobiotics derived from the <i<Lactobacillus gasseri</i< TMT36, TMT39 and TMT40 (HK36, HK39 or HK40) strains modulate the innate antiviral immune response of alveolar macrophages and reduce lung inflammatory damage triggered by TLR3 activation in vivo. Although our findings should be deepened and expanded, the results of the present work provide a scientific rationale for the use of nasally administered HK36, HK39 or HK40 to beneficially modulate TLR3-triggerd respiratory innate immune response. |
abstract_unstemmed |
Beneficial microbes with immunomodulatory capacities (immunobiotics) and their non-viable forms (postimmunobiotics) could be effectively utilized in formulations towards the prevention of respiratory viral infections. In this study, novel immunobiotic strains with the ability to increase antiviral immunity in porcine alveolar macrophages were selected from a library of <i<Lactobacillus gasseri</i<. Postimmunobiotics derived from the most remarkable strains were also evaluated in their capacity to modulate the immune response triggered by Toll-like receptor 3 (TLR3) in alveolar macrophages and to differentially regulate TLR3-mediated antiviral respiratory immunity in infant mice. We provide evidence that porcine alveolar macrophages (3D4/31 cells) are a useful in vitro tool for the screening of new antiviral immunobiotics and postimmunobiotics by assessing their ability to modulate the expression <i<IFN-β</i<, <i<IFN-λ1</i<, <i<RNAseL</i<, <i<Mx2</i<, and <i<IL-6</i<, which can be used as prospective biomarkers. We also demonstrate that the postimmunobiotics derived from the <i<Lactobacillus gasseri</i< TMT36, TMT39 and TMT40 (HK36, HK39 or HK40) strains modulate the innate antiviral immune response of alveolar macrophages and reduce lung inflammatory damage triggered by TLR3 activation in vivo. Although our findings should be deepened and expanded, the results of the present work provide a scientific rationale for the use of nasally administered HK36, HK39 or HK40 to beneficially modulate TLR3-triggerd respiratory innate immune response. |
collection_details |
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container_issue |
19, p 2986 |
title_short |
Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory Immunity |
url |
https://doi.org/10.3390/cells11192986 https://doaj.org/article/2ff7a67699f94eb3bc1fc79eac1c0470 https://www.mdpi.com/2073-4409/11/19/2986 https://doaj.org/toc/2073-4409 |
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author2 |
Fernanda Raya Tonetti Hikari Yamamuro Ryoko Shibata Kohtaro Fukuyama Nadia Gobbato Leonardo Albarracin Muhammad Shahid Riaz Rajoka A. K. M. Humayun Kober Wakako Ikeda-Ohtsubo Julio Villena Haruki Kitazawa |
author2Str |
Fernanda Raya Tonetti Hikari Yamamuro Ryoko Shibata Kohtaro Fukuyama Nadia Gobbato Leonardo Albarracin Muhammad Shahid Riaz Rajoka A. K. M. Humayun Kober Wakako Ikeda-Ohtsubo Julio Villena Haruki Kitazawa |
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callnumber-subject |
QH - Natural History and Biology |
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doi_str |
10.3390/cells11192986 |
callnumber-a |
QH573-671 |
up_date |
2024-07-03T20:36:42.514Z |
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