Assessment of The Imipenem Antibiotic Effects on The Expression of Staphylococcus Saprophyticus Serine-Aspartate Repeat Protein-Encoding Genes Isolated from Clinical Cases
Background: Staphylococcus saprophyticus is one of the most common causes of urinary tract infections (UTIs). Antibiotic resistance against imipenem is increasing worldwide. Our study aimed to investigate the effect of imipenem on expression of the S. saprophyticus serine-aspartate repeat protein-en...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Afsaneh Abedin [verfasserIn] Kumarss Amini [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2021 |
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| Schlagwörter: |
Staphylococcus saprophyticus, UTI, imipenem, antibiotic resistance |
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| Übergeordnetes Werk: |
In: Journal of Medical Bacteriology - Tehran University of Medical Sciences, 2012, 10(2021), 3-4 |
|---|---|
| Übergeordnetes Werk: |
volume:10 ; year:2021 ; number:3-4 |
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DOAJ086853619 |
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| 520 | |a Background: Staphylococcus saprophyticus is one of the most common causes of urinary tract infections (UTIs). Antibiotic resistance against imipenem is increasing worldwide. Our study aimed to investigate the effect of imipenem on expression of the S. saprophyticus serine-aspartate repeat protein-encoding genes by isolating multidrug-resistant strains. Methods: In this descriptive study, 500 specimens were randomly collected from clinical specimens. Firstly, isolates were identified using standard tests. Then, s. saprophyticus species harboring sdrC, D and E genes were detected using multiplex PCR. Antibiotic susceptibility testing and MIC values of imipenem performed and target genes expression detected by Real time PCR. Results: Out of 500 samples, 387 Staphylococcus species were isolated, among which 155 strains were S. saprophyticus species. PCR data indicated that 36.77% of S. saprophyticus isolates harbored one of sdr family genes. The MIC and subMIC values obtained for S. saprophyticus species treated with imipenem were 125 μg/ml and 225 µg/ml, respectively. Treatment with imipenem induced significant decrease in the expression of sdrC and sdrD genes as fold changes were -1.241 and -1.322, respectively. There was no statistically significant in sdrE gene expression. Conclusion: The results of our study showed that resistance to imipenem was significantly higher in strains harboring sdrE gene. Also, treatment with imipenem did not cause any significant change in the expression of sdrE gene, which could be a factor in the application of antibiotic resistance by bacteria to this antibiotic. In addition, the present study raises another alarm about the increased risk of antibiotic resistance | ||
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(DE-627)DOAJ086853619 (DE-599)DOAJa27efc4932834fd4ac6496673d8715b4 DE-627 ger DE-627 rakwb eng Afsaneh Abedin verfasserin aut Assessment of The Imipenem Antibiotic Effects on The Expression of Staphylococcus Saprophyticus Serine-Aspartate Repeat Protein-Encoding Genes Isolated from Clinical Cases 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Staphylococcus saprophyticus is one of the most common causes of urinary tract infections (UTIs). Antibiotic resistance against imipenem is increasing worldwide. Our study aimed to investigate the effect of imipenem on expression of the S. saprophyticus serine-aspartate repeat protein-encoding genes by isolating multidrug-resistant strains. Methods: In this descriptive study, 500 specimens were randomly collected from clinical specimens. Firstly, isolates were identified using standard tests. Then, s. saprophyticus species harboring sdrC, D and E genes were detected using multiplex PCR. Antibiotic susceptibility testing and MIC values of imipenem performed and target genes expression detected by Real time PCR. Results: Out of 500 samples, 387 Staphylococcus species were isolated, among which 155 strains were S. saprophyticus species. PCR data indicated that 36.77% of S. saprophyticus isolates harbored one of sdr family genes. The MIC and subMIC values obtained for S. saprophyticus species treated with imipenem were 125 μg/ml and 225 µg/ml, respectively. Treatment with imipenem induced significant decrease in the expression of sdrC and sdrD genes as fold changes were -1.241 and -1.322, respectively. There was no statistically significant in sdrE gene expression. Conclusion: The results of our study showed that resistance to imipenem was significantly higher in strains harboring sdrE gene. Also, treatment with imipenem did not cause any significant change in the expression of sdrE gene, which could be a factor in the application of antibiotic resistance by bacteria to this antibiotic. In addition, the present study raises another alarm about the increased risk of antibiotic resistance Staphylococcus saprophyticus, UTI, imipenem, antibiotic resistance Medicine R Kumarss Amini verfasserin aut In Journal of Medical Bacteriology Tehran University of Medical Sciences, 2012 10(2021), 3-4 (DE-627)734738188 (DE-600)2698544-5 23222581 nnns volume:10 year:2021 number:3-4 https://doaj.org/article/a27efc4932834fd4ac6496673d8715b4 kostenfrei https://jmb.tums.ac.ir/index.php/jmb/article/view/465 kostenfrei https://doaj.org/toc/2251-8649 Journal toc kostenfrei https://doaj.org/toc/2322-2581 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 3-4 |
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(DE-627)DOAJ086853619 (DE-599)DOAJa27efc4932834fd4ac6496673d8715b4 DE-627 ger DE-627 rakwb eng Afsaneh Abedin verfasserin aut Assessment of The Imipenem Antibiotic Effects on The Expression of Staphylococcus Saprophyticus Serine-Aspartate Repeat Protein-Encoding Genes Isolated from Clinical Cases 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Staphylococcus saprophyticus is one of the most common causes of urinary tract infections (UTIs). Antibiotic resistance against imipenem is increasing worldwide. Our study aimed to investigate the effect of imipenem on expression of the S. saprophyticus serine-aspartate repeat protein-encoding genes by isolating multidrug-resistant strains. Methods: In this descriptive study, 500 specimens were randomly collected from clinical specimens. Firstly, isolates were identified using standard tests. Then, s. saprophyticus species harboring sdrC, D and E genes were detected using multiplex PCR. Antibiotic susceptibility testing and MIC values of imipenem performed and target genes expression detected by Real time PCR. Results: Out of 500 samples, 387 Staphylococcus species were isolated, among which 155 strains were S. saprophyticus species. PCR data indicated that 36.77% of S. saprophyticus isolates harbored one of sdr family genes. The MIC and subMIC values obtained for S. saprophyticus species treated with imipenem were 125 μg/ml and 225 µg/ml, respectively. Treatment with imipenem induced significant decrease in the expression of sdrC and sdrD genes as fold changes were -1.241 and -1.322, respectively. There was no statistically significant in sdrE gene expression. Conclusion: The results of our study showed that resistance to imipenem was significantly higher in strains harboring sdrE gene. Also, treatment with imipenem did not cause any significant change in the expression of sdrE gene, which could be a factor in the application of antibiotic resistance by bacteria to this antibiotic. In addition, the present study raises another alarm about the increased risk of antibiotic resistance Staphylococcus saprophyticus, UTI, imipenem, antibiotic resistance Medicine R Kumarss Amini verfasserin aut In Journal of Medical Bacteriology Tehran University of Medical Sciences, 2012 10(2021), 3-4 (DE-627)734738188 (DE-600)2698544-5 23222581 nnns volume:10 year:2021 number:3-4 https://doaj.org/article/a27efc4932834fd4ac6496673d8715b4 kostenfrei https://jmb.tums.ac.ir/index.php/jmb/article/view/465 kostenfrei https://doaj.org/toc/2251-8649 Journal toc kostenfrei https://doaj.org/toc/2322-2581 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 3-4 |
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(DE-627)DOAJ086853619 (DE-599)DOAJa27efc4932834fd4ac6496673d8715b4 DE-627 ger DE-627 rakwb eng Afsaneh Abedin verfasserin aut Assessment of The Imipenem Antibiotic Effects on The Expression of Staphylococcus Saprophyticus Serine-Aspartate Repeat Protein-Encoding Genes Isolated from Clinical Cases 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Staphylococcus saprophyticus is one of the most common causes of urinary tract infections (UTIs). Antibiotic resistance against imipenem is increasing worldwide. Our study aimed to investigate the effect of imipenem on expression of the S. saprophyticus serine-aspartate repeat protein-encoding genes by isolating multidrug-resistant strains. Methods: In this descriptive study, 500 specimens were randomly collected from clinical specimens. Firstly, isolates were identified using standard tests. Then, s. saprophyticus species harboring sdrC, D and E genes were detected using multiplex PCR. Antibiotic susceptibility testing and MIC values of imipenem performed and target genes expression detected by Real time PCR. Results: Out of 500 samples, 387 Staphylococcus species were isolated, among which 155 strains were S. saprophyticus species. PCR data indicated that 36.77% of S. saprophyticus isolates harbored one of sdr family genes. The MIC and subMIC values obtained for S. saprophyticus species treated with imipenem were 125 μg/ml and 225 µg/ml, respectively. Treatment with imipenem induced significant decrease in the expression of sdrC and sdrD genes as fold changes were -1.241 and -1.322, respectively. There was no statistically significant in sdrE gene expression. Conclusion: The results of our study showed that resistance to imipenem was significantly higher in strains harboring sdrE gene. Also, treatment with imipenem did not cause any significant change in the expression of sdrE gene, which could be a factor in the application of antibiotic resistance by bacteria to this antibiotic. In addition, the present study raises another alarm about the increased risk of antibiotic resistance Staphylococcus saprophyticus, UTI, imipenem, antibiotic resistance Medicine R Kumarss Amini verfasserin aut In Journal of Medical Bacteriology Tehran University of Medical Sciences, 2012 10(2021), 3-4 (DE-627)734738188 (DE-600)2698544-5 23222581 nnns volume:10 year:2021 number:3-4 https://doaj.org/article/a27efc4932834fd4ac6496673d8715b4 kostenfrei https://jmb.tums.ac.ir/index.php/jmb/article/view/465 kostenfrei https://doaj.org/toc/2251-8649 Journal toc kostenfrei https://doaj.org/toc/2322-2581 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 3-4 |
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(DE-627)DOAJ086853619 (DE-599)DOAJa27efc4932834fd4ac6496673d8715b4 DE-627 ger DE-627 rakwb eng Afsaneh Abedin verfasserin aut Assessment of The Imipenem Antibiotic Effects on The Expression of Staphylococcus Saprophyticus Serine-Aspartate Repeat Protein-Encoding Genes Isolated from Clinical Cases 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Staphylococcus saprophyticus is one of the most common causes of urinary tract infections (UTIs). Antibiotic resistance against imipenem is increasing worldwide. Our study aimed to investigate the effect of imipenem on expression of the S. saprophyticus serine-aspartate repeat protein-encoding genes by isolating multidrug-resistant strains. Methods: In this descriptive study, 500 specimens were randomly collected from clinical specimens. Firstly, isolates were identified using standard tests. Then, s. saprophyticus species harboring sdrC, D and E genes were detected using multiplex PCR. Antibiotic susceptibility testing and MIC values of imipenem performed and target genes expression detected by Real time PCR. Results: Out of 500 samples, 387 Staphylococcus species were isolated, among which 155 strains were S. saprophyticus species. PCR data indicated that 36.77% of S. saprophyticus isolates harbored one of sdr family genes. The MIC and subMIC values obtained for S. saprophyticus species treated with imipenem were 125 μg/ml and 225 µg/ml, respectively. Treatment with imipenem induced significant decrease in the expression of sdrC and sdrD genes as fold changes were -1.241 and -1.322, respectively. There was no statistically significant in sdrE gene expression. Conclusion: The results of our study showed that resistance to imipenem was significantly higher in strains harboring sdrE gene. Also, treatment with imipenem did not cause any significant change in the expression of sdrE gene, which could be a factor in the application of antibiotic resistance by bacteria to this antibiotic. In addition, the present study raises another alarm about the increased risk of antibiotic resistance Staphylococcus saprophyticus, UTI, imipenem, antibiotic resistance Medicine R Kumarss Amini verfasserin aut In Journal of Medical Bacteriology Tehran University of Medical Sciences, 2012 10(2021), 3-4 (DE-627)734738188 (DE-600)2698544-5 23222581 nnns volume:10 year:2021 number:3-4 https://doaj.org/article/a27efc4932834fd4ac6496673d8715b4 kostenfrei https://jmb.tums.ac.ir/index.php/jmb/article/view/465 kostenfrei https://doaj.org/toc/2251-8649 Journal toc kostenfrei https://doaj.org/toc/2322-2581 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 3-4 |
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(DE-627)DOAJ086853619 (DE-599)DOAJa27efc4932834fd4ac6496673d8715b4 DE-627 ger DE-627 rakwb eng Afsaneh Abedin verfasserin aut Assessment of The Imipenem Antibiotic Effects on The Expression of Staphylococcus Saprophyticus Serine-Aspartate Repeat Protein-Encoding Genes Isolated from Clinical Cases 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Staphylococcus saprophyticus is one of the most common causes of urinary tract infections (UTIs). Antibiotic resistance against imipenem is increasing worldwide. Our study aimed to investigate the effect of imipenem on expression of the S. saprophyticus serine-aspartate repeat protein-encoding genes by isolating multidrug-resistant strains. Methods: In this descriptive study, 500 specimens were randomly collected from clinical specimens. Firstly, isolates were identified using standard tests. Then, s. saprophyticus species harboring sdrC, D and E genes were detected using multiplex PCR. Antibiotic susceptibility testing and MIC values of imipenem performed and target genes expression detected by Real time PCR. Results: Out of 500 samples, 387 Staphylococcus species were isolated, among which 155 strains were S. saprophyticus species. PCR data indicated that 36.77% of S. saprophyticus isolates harbored one of sdr family genes. The MIC and subMIC values obtained for S. saprophyticus species treated with imipenem were 125 μg/ml and 225 µg/ml, respectively. Treatment with imipenem induced significant decrease in the expression of sdrC and sdrD genes as fold changes were -1.241 and -1.322, respectively. There was no statistically significant in sdrE gene expression. Conclusion: The results of our study showed that resistance to imipenem was significantly higher in strains harboring sdrE gene. Also, treatment with imipenem did not cause any significant change in the expression of sdrE gene, which could be a factor in the application of antibiotic resistance by bacteria to this antibiotic. In addition, the present study raises another alarm about the increased risk of antibiotic resistance Staphylococcus saprophyticus, UTI, imipenem, antibiotic resistance Medicine R Kumarss Amini verfasserin aut In Journal of Medical Bacteriology Tehran University of Medical Sciences, 2012 10(2021), 3-4 (DE-627)734738188 (DE-600)2698544-5 23222581 nnns volume:10 year:2021 number:3-4 https://doaj.org/article/a27efc4932834fd4ac6496673d8715b4 kostenfrei https://jmb.tums.ac.ir/index.php/jmb/article/view/465 kostenfrei https://doaj.org/toc/2251-8649 Journal toc kostenfrei https://doaj.org/toc/2322-2581 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 3-4 |
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Assessment of The Imipenem Antibiotic Effects on The Expression of Staphylococcus Saprophyticus Serine-Aspartate Repeat Protein-Encoding Genes Isolated from Clinical Cases Staphylococcus saprophyticus, UTI, imipenem, antibiotic resistance |
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assessment of the imipenem antibiotic effects on the expression of staphylococcus saprophyticus serine-aspartate repeat protein-encoding genes isolated from clinical cases |
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Assessment of The Imipenem Antibiotic Effects on The Expression of Staphylococcus Saprophyticus Serine-Aspartate Repeat Protein-Encoding Genes Isolated from Clinical Cases |
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Background: Staphylococcus saprophyticus is one of the most common causes of urinary tract infections (UTIs). Antibiotic resistance against imipenem is increasing worldwide. Our study aimed to investigate the effect of imipenem on expression of the S. saprophyticus serine-aspartate repeat protein-encoding genes by isolating multidrug-resistant strains. Methods: In this descriptive study, 500 specimens were randomly collected from clinical specimens. Firstly, isolates were identified using standard tests. Then, s. saprophyticus species harboring sdrC, D and E genes were detected using multiplex PCR. Antibiotic susceptibility testing and MIC values of imipenem performed and target genes expression detected by Real time PCR. Results: Out of 500 samples, 387 Staphylococcus species were isolated, among which 155 strains were S. saprophyticus species. PCR data indicated that 36.77% of S. saprophyticus isolates harbored one of sdr family genes. The MIC and subMIC values obtained for S. saprophyticus species treated with imipenem were 125 μg/ml and 225 µg/ml, respectively. Treatment with imipenem induced significant decrease in the expression of sdrC and sdrD genes as fold changes were -1.241 and -1.322, respectively. There was no statistically significant in sdrE gene expression. Conclusion: The results of our study showed that resistance to imipenem was significantly higher in strains harboring sdrE gene. Also, treatment with imipenem did not cause any significant change in the expression of sdrE gene, which could be a factor in the application of antibiotic resistance by bacteria to this antibiotic. In addition, the present study raises another alarm about the increased risk of antibiotic resistance |
| abstractGer |
Background: Staphylococcus saprophyticus is one of the most common causes of urinary tract infections (UTIs). Antibiotic resistance against imipenem is increasing worldwide. Our study aimed to investigate the effect of imipenem on expression of the S. saprophyticus serine-aspartate repeat protein-encoding genes by isolating multidrug-resistant strains. Methods: In this descriptive study, 500 specimens were randomly collected from clinical specimens. Firstly, isolates were identified using standard tests. Then, s. saprophyticus species harboring sdrC, D and E genes were detected using multiplex PCR. Antibiotic susceptibility testing and MIC values of imipenem performed and target genes expression detected by Real time PCR. Results: Out of 500 samples, 387 Staphylococcus species were isolated, among which 155 strains were S. saprophyticus species. PCR data indicated that 36.77% of S. saprophyticus isolates harbored one of sdr family genes. The MIC and subMIC values obtained for S. saprophyticus species treated with imipenem were 125 μg/ml and 225 µg/ml, respectively. Treatment with imipenem induced significant decrease in the expression of sdrC and sdrD genes as fold changes were -1.241 and -1.322, respectively. There was no statistically significant in sdrE gene expression. Conclusion: The results of our study showed that resistance to imipenem was significantly higher in strains harboring sdrE gene. Also, treatment with imipenem did not cause any significant change in the expression of sdrE gene, which could be a factor in the application of antibiotic resistance by bacteria to this antibiotic. In addition, the present study raises another alarm about the increased risk of antibiotic resistance |
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Background: Staphylococcus saprophyticus is one of the most common causes of urinary tract infections (UTIs). Antibiotic resistance against imipenem is increasing worldwide. Our study aimed to investigate the effect of imipenem on expression of the S. saprophyticus serine-aspartate repeat protein-encoding genes by isolating multidrug-resistant strains. Methods: In this descriptive study, 500 specimens were randomly collected from clinical specimens. Firstly, isolates were identified using standard tests. Then, s. saprophyticus species harboring sdrC, D and E genes were detected using multiplex PCR. Antibiotic susceptibility testing and MIC values of imipenem performed and target genes expression detected by Real time PCR. Results: Out of 500 samples, 387 Staphylococcus species were isolated, among which 155 strains were S. saprophyticus species. PCR data indicated that 36.77% of S. saprophyticus isolates harbored one of sdr family genes. The MIC and subMIC values obtained for S. saprophyticus species treated with imipenem were 125 μg/ml and 225 µg/ml, respectively. Treatment with imipenem induced significant decrease in the expression of sdrC and sdrD genes as fold changes were -1.241 and -1.322, respectively. There was no statistically significant in sdrE gene expression. Conclusion: The results of our study showed that resistance to imipenem was significantly higher in strains harboring sdrE gene. Also, treatment with imipenem did not cause any significant change in the expression of sdrE gene, which could be a factor in the application of antibiotic resistance by bacteria to this antibiotic. In addition, the present study raises another alarm about the increased risk of antibiotic resistance |
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