Detection of Different Types of Class 1, 2 and 3 Integrons among Pseudomonas aeruginosa Isolates from Raw Milks

Background: Pseudomonas aeruginosa is a leading cause of hospital-acquired infections that causes severe diseases in immuno-compromised individuals. integrons have a major role in multidrug resistanct, diversity, evolution and recombination strains. Various animals may act as the reservoir for...
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Autor*in:	Parisa Mobasseri [verfasserIn] Mahdi Jamali Harsini [verfasserIn] Sedigeh Mehrabian [verfasserIn] Kumarss Amini [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	Pseudomonas aeruginosa class 1 integron class 2 integron class 3 integron Antibiotic resistance PCR

Übergeordnetes Werk:	In: Journal of Medical Bacteriology - Tehran University of Medical Sciences, 2012, 10(2021), 3-4
Übergeordnetes Werk:	volume:10 ; year:2021 ; number:3-4

Links:	Link aufrufen Link aufrufen Journal toc Journal toc

Katalog-ID:	DOAJ086853643

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520			\|a Background: Pseudomonas aeruginosa is a leading cause of hospital-acquired infections that causes severe diseases in immuno-compromised individuals. integrons have a major role in multidrug resistanct, diversity, evolution and recombination strains. Various animals may act as the reservoir for bacterial humans pathogens. This study is aimed to evaluate the frequency of class 1, 2 and 3 integrons in P. aeruginosa isolates detected in raw milks. Methods: Identification of isolates were confirmed with morphology, Gram staining and biochemical tests. Drug resistance to various antibiotics was investigated using agar disk diffusion method. After DNA extraction of the isolates, they were subjected to a polymerase chain reaction for detection of class 1, 2 and 3 integrons. Results: In this study, 60 P. aeruginosa isolates were isolated from raw milk samples. The isolates showed resistance to amikacin (100%), ampicillin (100%), gentamicin (86.6%), cefotaxime (10%), ciprofloxacin (6.6%) and ceftazidime (3.3%). PCR analysis revealed the presence of intI-1in 49(81.6%), intI-2 in 9(15%), and intI-3 in 31(51.6%) isolates. Furthermore, class 1 and class 2 integrons were detected in 8(13.3%). In place, class 1 and class 3 integrons were observed in 26(43.3%) and class 2 and class 3 integrons in 6(10%) isolates. Conclusion: Ciprofloxacin and ceftazidime were the most effective antibiotics against P. aeruginosai isolates in this study. The distribution of different classes of integrons in this study was high and it sheds light on the importance of regulations on the antibiotic uses.
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allfields	(DE-627)DOAJ086853643 (DE-599)DOAJ9d2cbec7a3b04c37899d6e507ff8f7df DE-627 ger DE-627 rakwb eng Parisa Mobasseri verfasserin aut Detection of Different Types of Class 1, 2 and 3 Integrons among Pseudomonas aeruginosa Isolates from Raw Milks 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Pseudomonas aeruginosa is a leading cause of hospital-acquired infections that causes severe diseases in immuno-compromised individuals. integrons have a major role in multidrug resistanct, diversity, evolution and recombination strains. Various animals may act as the reservoir for bacterial humans pathogens. This study is aimed to evaluate the frequency of class 1, 2 and 3 integrons in P. aeruginosa isolates detected in raw milks. Methods: Identification of isolates were confirmed with morphology, Gram staining and biochemical tests. Drug resistance to various antibiotics was investigated using agar disk diffusion method. After DNA extraction of the isolates, they were subjected to a polymerase chain reaction for detection of class 1, 2 and 3 integrons. Results: In this study, 60 P. aeruginosa isolates were isolated from raw milk samples. The isolates showed resistance to amikacin (100%), ampicillin (100%), gentamicin (86.6%), cefotaxime (10%), ciprofloxacin (6.6%) and ceftazidime (3.3%). PCR analysis revealed the presence of intI-1in 49(81.6%), intI-2 in 9(15%), and intI-3 in 31(51.6%) isolates. Furthermore, class 1 and class 2 integrons were detected in 8(13.3%). In place, class 1 and class 3 integrons were observed in 26(43.3%) and class 2 and class 3 integrons in 6(10%) isolates. Conclusion: Ciprofloxacin and ceftazidime were the most effective antibiotics against P. aeruginosai isolates in this study. The distribution of different classes of integrons in this study was high and it sheds light on the importance of regulations on the antibiotic uses. Pseudomonas aeruginosa class 1 integron class 2 integron class 3 integron Antibiotic resistance PCR Medicine R Mahdi Jamali Harsini verfasserin aut Sedigeh Mehrabian verfasserin aut Kumarss Amini verfasserin aut In Journal of Medical Bacteriology Tehran University of Medical Sciences, 2012 10(2021), 3-4 (DE-627)734738188 (DE-600)2698544-5 23222581 nnns volume:10 year:2021 number:3-4 https://doaj.org/article/9d2cbec7a3b04c37899d6e507ff8f7df kostenfrei https://jmb.tums.ac.ir/index.php/jmb/article/view/460 kostenfrei https://doaj.org/toc/2251-8649 Journal toc kostenfrei https://doaj.org/toc/2322-2581 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 3-4
spelling	(DE-627)DOAJ086853643 (DE-599)DOAJ9d2cbec7a3b04c37899d6e507ff8f7df DE-627 ger DE-627 rakwb eng Parisa Mobasseri verfasserin aut Detection of Different Types of Class 1, 2 and 3 Integrons among Pseudomonas aeruginosa Isolates from Raw Milks 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Pseudomonas aeruginosa is a leading cause of hospital-acquired infections that causes severe diseases in immuno-compromised individuals. integrons have a major role in multidrug resistanct, diversity, evolution and recombination strains. Various animals may act as the reservoir for bacterial humans pathogens. This study is aimed to evaluate the frequency of class 1, 2 and 3 integrons in P. aeruginosa isolates detected in raw milks. Methods: Identification of isolates were confirmed with morphology, Gram staining and biochemical tests. Drug resistance to various antibiotics was investigated using agar disk diffusion method. After DNA extraction of the isolates, they were subjected to a polymerase chain reaction for detection of class 1, 2 and 3 integrons. Results: In this study, 60 P. aeruginosa isolates were isolated from raw milk samples. The isolates showed resistance to amikacin (100%), ampicillin (100%), gentamicin (86.6%), cefotaxime (10%), ciprofloxacin (6.6%) and ceftazidime (3.3%). PCR analysis revealed the presence of intI-1in 49(81.6%), intI-2 in 9(15%), and intI-3 in 31(51.6%) isolates. Furthermore, class 1 and class 2 integrons were detected in 8(13.3%). In place, class 1 and class 3 integrons were observed in 26(43.3%) and class 2 and class 3 integrons in 6(10%) isolates. Conclusion: Ciprofloxacin and ceftazidime were the most effective antibiotics against P. aeruginosai isolates in this study. The distribution of different classes of integrons in this study was high and it sheds light on the importance of regulations on the antibiotic uses. Pseudomonas aeruginosa class 1 integron class 2 integron class 3 integron Antibiotic resistance PCR Medicine R Mahdi Jamali Harsini verfasserin aut Sedigeh Mehrabian verfasserin aut Kumarss Amini verfasserin aut In Journal of Medical Bacteriology Tehran University of Medical Sciences, 2012 10(2021), 3-4 (DE-627)734738188 (DE-600)2698544-5 23222581 nnns volume:10 year:2021 number:3-4 https://doaj.org/article/9d2cbec7a3b04c37899d6e507ff8f7df kostenfrei https://jmb.tums.ac.ir/index.php/jmb/article/view/460 kostenfrei https://doaj.org/toc/2251-8649 Journal toc kostenfrei https://doaj.org/toc/2322-2581 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 3-4
allfields_unstemmed	(DE-627)DOAJ086853643 (DE-599)DOAJ9d2cbec7a3b04c37899d6e507ff8f7df DE-627 ger DE-627 rakwb eng Parisa Mobasseri verfasserin aut Detection of Different Types of Class 1, 2 and 3 Integrons among Pseudomonas aeruginosa Isolates from Raw Milks 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Pseudomonas aeruginosa is a leading cause of hospital-acquired infections that causes severe diseases in immuno-compromised individuals. integrons have a major role in multidrug resistanct, diversity, evolution and recombination strains. Various animals may act as the reservoir for bacterial humans pathogens. This study is aimed to evaluate the frequency of class 1, 2 and 3 integrons in P. aeruginosa isolates detected in raw milks. Methods: Identification of isolates were confirmed with morphology, Gram staining and biochemical tests. Drug resistance to various antibiotics was investigated using agar disk diffusion method. After DNA extraction of the isolates, they were subjected to a polymerase chain reaction for detection of class 1, 2 and 3 integrons. Results: In this study, 60 P. aeruginosa isolates were isolated from raw milk samples. The isolates showed resistance to amikacin (100%), ampicillin (100%), gentamicin (86.6%), cefotaxime (10%), ciprofloxacin (6.6%) and ceftazidime (3.3%). PCR analysis revealed the presence of intI-1in 49(81.6%), intI-2 in 9(15%), and intI-3 in 31(51.6%) isolates. Furthermore, class 1 and class 2 integrons were detected in 8(13.3%). In place, class 1 and class 3 integrons were observed in 26(43.3%) and class 2 and class 3 integrons in 6(10%) isolates. Conclusion: Ciprofloxacin and ceftazidime were the most effective antibiotics against P. aeruginosai isolates in this study. The distribution of different classes of integrons in this study was high and it sheds light on the importance of regulations on the antibiotic uses. Pseudomonas aeruginosa class 1 integron class 2 integron class 3 integron Antibiotic resistance PCR Medicine R Mahdi Jamali Harsini verfasserin aut Sedigeh Mehrabian verfasserin aut Kumarss Amini verfasserin aut In Journal of Medical Bacteriology Tehran University of Medical Sciences, 2012 10(2021), 3-4 (DE-627)734738188 (DE-600)2698544-5 23222581 nnns volume:10 year:2021 number:3-4 https://doaj.org/article/9d2cbec7a3b04c37899d6e507ff8f7df kostenfrei https://jmb.tums.ac.ir/index.php/jmb/article/view/460 kostenfrei https://doaj.org/toc/2251-8649 Journal toc kostenfrei https://doaj.org/toc/2322-2581 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 3-4
allfieldsGer	(DE-627)DOAJ086853643 (DE-599)DOAJ9d2cbec7a3b04c37899d6e507ff8f7df DE-627 ger DE-627 rakwb eng Parisa Mobasseri verfasserin aut Detection of Different Types of Class 1, 2 and 3 Integrons among Pseudomonas aeruginosa Isolates from Raw Milks 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Pseudomonas aeruginosa is a leading cause of hospital-acquired infections that causes severe diseases in immuno-compromised individuals. integrons have a major role in multidrug resistanct, diversity, evolution and recombination strains. Various animals may act as the reservoir for bacterial humans pathogens. This study is aimed to evaluate the frequency of class 1, 2 and 3 integrons in P. aeruginosa isolates detected in raw milks. Methods: Identification of isolates were confirmed with morphology, Gram staining and biochemical tests. Drug resistance to various antibiotics was investigated using agar disk diffusion method. After DNA extraction of the isolates, they were subjected to a polymerase chain reaction for detection of class 1, 2 and 3 integrons. Results: In this study, 60 P. aeruginosa isolates were isolated from raw milk samples. The isolates showed resistance to amikacin (100%), ampicillin (100%), gentamicin (86.6%), cefotaxime (10%), ciprofloxacin (6.6%) and ceftazidime (3.3%). PCR analysis revealed the presence of intI-1in 49(81.6%), intI-2 in 9(15%), and intI-3 in 31(51.6%) isolates. Furthermore, class 1 and class 2 integrons were detected in 8(13.3%). In place, class 1 and class 3 integrons were observed in 26(43.3%) and class 2 and class 3 integrons in 6(10%) isolates. Conclusion: Ciprofloxacin and ceftazidime were the most effective antibiotics against P. aeruginosai isolates in this study. The distribution of different classes of integrons in this study was high and it sheds light on the importance of regulations on the antibiotic uses. Pseudomonas aeruginosa class 1 integron class 2 integron class 3 integron Antibiotic resistance PCR Medicine R Mahdi Jamali Harsini verfasserin aut Sedigeh Mehrabian verfasserin aut Kumarss Amini verfasserin aut In Journal of Medical Bacteriology Tehran University of Medical Sciences, 2012 10(2021), 3-4 (DE-627)734738188 (DE-600)2698544-5 23222581 nnns volume:10 year:2021 number:3-4 https://doaj.org/article/9d2cbec7a3b04c37899d6e507ff8f7df kostenfrei https://jmb.tums.ac.ir/index.php/jmb/article/view/460 kostenfrei https://doaj.org/toc/2251-8649 Journal toc kostenfrei https://doaj.org/toc/2322-2581 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 3-4
allfieldsSound	(DE-627)DOAJ086853643 (DE-599)DOAJ9d2cbec7a3b04c37899d6e507ff8f7df DE-627 ger DE-627 rakwb eng Parisa Mobasseri verfasserin aut Detection of Different Types of Class 1, 2 and 3 Integrons among Pseudomonas aeruginosa Isolates from Raw Milks 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Pseudomonas aeruginosa is a leading cause of hospital-acquired infections that causes severe diseases in immuno-compromised individuals. integrons have a major role in multidrug resistanct, diversity, evolution and recombination strains. Various animals may act as the reservoir for bacterial humans pathogens. This study is aimed to evaluate the frequency of class 1, 2 and 3 integrons in P. aeruginosa isolates detected in raw milks. Methods: Identification of isolates were confirmed with morphology, Gram staining and biochemical tests. Drug resistance to various antibiotics was investigated using agar disk diffusion method. After DNA extraction of the isolates, they were subjected to a polymerase chain reaction for detection of class 1, 2 and 3 integrons. Results: In this study, 60 P. aeruginosa isolates were isolated from raw milk samples. The isolates showed resistance to amikacin (100%), ampicillin (100%), gentamicin (86.6%), cefotaxime (10%), ciprofloxacin (6.6%) and ceftazidime (3.3%). PCR analysis revealed the presence of intI-1in 49(81.6%), intI-2 in 9(15%), and intI-3 in 31(51.6%) isolates. Furthermore, class 1 and class 2 integrons were detected in 8(13.3%). In place, class 1 and class 3 integrons were observed in 26(43.3%) and class 2 and class 3 integrons in 6(10%) isolates. Conclusion: Ciprofloxacin and ceftazidime were the most effective antibiotics against P. aeruginosai isolates in this study. The distribution of different classes of integrons in this study was high and it sheds light on the importance of regulations on the antibiotic uses. Pseudomonas aeruginosa class 1 integron class 2 integron class 3 integron Antibiotic resistance PCR Medicine R Mahdi Jamali Harsini verfasserin aut Sedigeh Mehrabian verfasserin aut Kumarss Amini verfasserin aut In Journal of Medical Bacteriology Tehran University of Medical Sciences, 2012 10(2021), 3-4 (DE-627)734738188 (DE-600)2698544-5 23222581 nnns volume:10 year:2021 number:3-4 https://doaj.org/article/9d2cbec7a3b04c37899d6e507ff8f7df kostenfrei https://jmb.tums.ac.ir/index.php/jmb/article/view/460 kostenfrei https://doaj.org/toc/2251-8649 Journal toc kostenfrei https://doaj.org/toc/2322-2581 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 3-4
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author	Parisa Mobasseri
spellingShingle	Parisa Mobasseri misc Pseudomonas aeruginosa misc class 1 integron misc class 2 integron misc class 3 integron misc Antibiotic resistance misc PCR misc Medicine misc R Detection of Different Types of Class 1, 2 and 3 Integrons among Pseudomonas aeruginosa Isolates from Raw Milks
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topic_title	Detection of Different Types of Class 1, 2 and 3 Integrons among Pseudomonas aeruginosa Isolates from Raw Milks Pseudomonas aeruginosa class 1 integron class 2 integron class 3 integron Antibiotic resistance PCR
topic	misc Pseudomonas aeruginosa misc class 1 integron misc class 2 integron misc class 3 integron misc Antibiotic resistance misc PCR misc Medicine misc R
topic_unstemmed	misc Pseudomonas aeruginosa misc class 1 integron misc class 2 integron misc class 3 integron misc Antibiotic resistance misc PCR misc Medicine misc R
topic_browse	misc Pseudomonas aeruginosa misc class 1 integron misc class 2 integron misc class 3 integron misc Antibiotic resistance misc PCR misc Medicine misc R
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title	Detection of Different Types of Class 1, 2 and 3 Integrons among Pseudomonas aeruginosa Isolates from Raw Milks
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title_full	Detection of Different Types of Class 1, 2 and 3 Integrons among Pseudomonas aeruginosa Isolates from Raw Milks
author_sort	Parisa Mobasseri
journal	Journal of Medical Bacteriology
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author_browse	Parisa Mobasseri Mahdi Jamali Harsini Sedigeh Mehrabian Kumarss Amini
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title_sort	detection of different types of class 1, 2 and 3 integrons among pseudomonas aeruginosa isolates from raw milks
title_auth	Detection of Different Types of Class 1, 2 and 3 Integrons among Pseudomonas aeruginosa Isolates from Raw Milks
abstract	Background: Pseudomonas aeruginosa is a leading cause of hospital-acquired infections that causes severe diseases in immuno-compromised individuals. integrons have a major role in multidrug resistanct, diversity, evolution and recombination strains. Various animals may act as the reservoir for bacterial humans pathogens. This study is aimed to evaluate the frequency of class 1, 2 and 3 integrons in P. aeruginosa isolates detected in raw milks. Methods: Identification of isolates were confirmed with morphology, Gram staining and biochemical tests. Drug resistance to various antibiotics was investigated using agar disk diffusion method. After DNA extraction of the isolates, they were subjected to a polymerase chain reaction for detection of class 1, 2 and 3 integrons. Results: In this study, 60 P. aeruginosa isolates were isolated from raw milk samples. The isolates showed resistance to amikacin (100%), ampicillin (100%), gentamicin (86.6%), cefotaxime (10%), ciprofloxacin (6.6%) and ceftazidime (3.3%). PCR analysis revealed the presence of intI-1in 49(81.6%), intI-2 in 9(15%), and intI-3 in 31(51.6%) isolates. Furthermore, class 1 and class 2 integrons were detected in 8(13.3%). In place, class 1 and class 3 integrons were observed in 26(43.3%) and class 2 and class 3 integrons in 6(10%) isolates. Conclusion: Ciprofloxacin and ceftazidime were the most effective antibiotics against P. aeruginosai isolates in this study. The distribution of different classes of integrons in this study was high and it sheds light on the importance of regulations on the antibiotic uses.
abstractGer	Background: Pseudomonas aeruginosa is a leading cause of hospital-acquired infections that causes severe diseases in immuno-compromised individuals. integrons have a major role in multidrug resistanct, diversity, evolution and recombination strains. Various animals may act as the reservoir for bacterial humans pathogens. This study is aimed to evaluate the frequency of class 1, 2 and 3 integrons in P. aeruginosa isolates detected in raw milks. Methods: Identification of isolates were confirmed with morphology, Gram staining and biochemical tests. Drug resistance to various antibiotics was investigated using agar disk diffusion method. After DNA extraction of the isolates, they were subjected to a polymerase chain reaction for detection of class 1, 2 and 3 integrons. Results: In this study, 60 P. aeruginosa isolates were isolated from raw milk samples. The isolates showed resistance to amikacin (100%), ampicillin (100%), gentamicin (86.6%), cefotaxime (10%), ciprofloxacin (6.6%) and ceftazidime (3.3%). PCR analysis revealed the presence of intI-1in 49(81.6%), intI-2 in 9(15%), and intI-3 in 31(51.6%) isolates. Furthermore, class 1 and class 2 integrons were detected in 8(13.3%). In place, class 1 and class 3 integrons were observed in 26(43.3%) and class 2 and class 3 integrons in 6(10%) isolates. Conclusion: Ciprofloxacin and ceftazidime were the most effective antibiotics against P. aeruginosai isolates in this study. The distribution of different classes of integrons in this study was high and it sheds light on the importance of regulations on the antibiotic uses.
abstract_unstemmed	Background: Pseudomonas aeruginosa is a leading cause of hospital-acquired infections that causes severe diseases in immuno-compromised individuals. integrons have a major role in multidrug resistanct, diversity, evolution and recombination strains. Various animals may act as the reservoir for bacterial humans pathogens. This study is aimed to evaluate the frequency of class 1, 2 and 3 integrons in P. aeruginosa isolates detected in raw milks. Methods: Identification of isolates were confirmed with morphology, Gram staining and biochemical tests. Drug resistance to various antibiotics was investigated using agar disk diffusion method. After DNA extraction of the isolates, they were subjected to a polymerase chain reaction for detection of class 1, 2 and 3 integrons. Results: In this study, 60 P. aeruginosa isolates were isolated from raw milk samples. The isolates showed resistance to amikacin (100%), ampicillin (100%), gentamicin (86.6%), cefotaxime (10%), ciprofloxacin (6.6%) and ceftazidime (3.3%). PCR analysis revealed the presence of intI-1in 49(81.6%), intI-2 in 9(15%), and intI-3 in 31(51.6%) isolates. Furthermore, class 1 and class 2 integrons were detected in 8(13.3%). In place, class 1 and class 3 integrons were observed in 26(43.3%) and class 2 and class 3 integrons in 6(10%) isolates. Conclusion: Ciprofloxacin and ceftazidime were the most effective antibiotics against P. aeruginosai isolates in this study. The distribution of different classes of integrons in this study was high and it sheds light on the importance of regulations on the antibiotic uses.
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title_short	Detection of Different Types of Class 1, 2 and 3 Integrons among Pseudomonas aeruginosa Isolates from Raw Milks
url	https://doaj.org/article/9d2cbec7a3b04c37899d6e507ff8f7df https://jmb.tums.ac.ir/index.php/jmb/article/view/460 https://doaj.org/toc/2251-8649 https://doaj.org/toc/2322-2581
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Detection of Different Types of Class 1, 2 and 3 Integrons among Pseudomonas aeruginosa Isolates from Raw Milks

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