Our Experiences With B Cell Clonality Analysis Using Multiplex Pcr Amplification in Paraffin-Embedded Tissue

The clonal determination of B-cell lymphoproliferative diseases by immunoglobulin heavy chain (IgH) rearrangement by polymerase chain reaction (PCR) is widely used. In this study we report our experiences with B cell clonality analysis using multiplex PCR amplification followed by heteroduplex analy...
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Autor*in:	Stanek L. [verfasserIn] Llsova S. [verfasserIn] Tvrdlk D. [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2014

Schlagwörter:	monoclonality pcr igh paraffin-embedded tissue heteroduplex

Übergeordnetes Werk:	In: Acta Medica Martiniana - Sciendo, 2011, 14(2014), 1, Seite 5-13
Übergeordnetes Werk:	volume:14 ; year:2014 ; number:1 ; pages:5-13

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.2478/acm-2014-0001

Katalog-ID:	DOAJ087176335

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author	Stanek L.
spellingShingle	Stanek L. misc monoclonality misc pcr misc igh misc paraffin-embedded tissue misc heteroduplex misc Medicine misc R Our Experiences With B Cell Clonality Analysis Using Multiplex Pcr Amplification in Paraffin-Embedded Tissue
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topic_title	Our Experiences With B Cell Clonality Analysis Using Multiplex Pcr Amplification in Paraffin-Embedded Tissue monoclonality pcr igh paraffin-embedded tissue heteroduplex
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title	Our Experiences With B Cell Clonality Analysis Using Multiplex Pcr Amplification in Paraffin-Embedded Tissue
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title_sort	our experiences with b cell clonality analysis using multiplex pcr amplification in paraffin-embedded tissue
title_auth	Our Experiences With B Cell Clonality Analysis Using Multiplex Pcr Amplification in Paraffin-Embedded Tissue
abstract	The clonal determination of B-cell lymphoproliferative diseases by immunoglobulin heavy chain (IgH) rearrangement by polymerase chain reaction (PCR) is widely used. In this study we report our experiences with B cell clonality analysis using multiplex PCR amplification followed by heteroduplex analysis, which was found to be essential for the efficient resolution of monoclonal bands within polyclonal backgrounds, in formalin-fixed paraffinembedded tissue.
abstractGer	The clonal determination of B-cell lymphoproliferative diseases by immunoglobulin heavy chain (IgH) rearrangement by polymerase chain reaction (PCR) is widely used. In this study we report our experiences with B cell clonality analysis using multiplex PCR amplification followed by heteroduplex analysis, which was found to be essential for the efficient resolution of monoclonal bands within polyclonal backgrounds, in formalin-fixed paraffinembedded tissue.
abstract_unstemmed	The clonal determination of B-cell lymphoproliferative diseases by immunoglobulin heavy chain (IgH) rearrangement by polymerase chain reaction (PCR) is widely used. In this study we report our experiences with B cell clonality analysis using multiplex PCR amplification followed by heteroduplex analysis, which was found to be essential for the efficient resolution of monoclonal bands within polyclonal backgrounds, in formalin-fixed paraffinembedded tissue.
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title_short	Our Experiences With B Cell Clonality Analysis Using Multiplex Pcr Amplification in Paraffin-Embedded Tissue
url	https://doi.org/10.2478/acm-2014-0001 https://doaj.org/article/a649354747094d0bba41a13356dcae99 https://doaj.org/toc/1335-8421
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