Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification

The polymerase chain reaction (PCR) is essential in nucleic acid amplification tests and is widely used in many applications such as infectious disease detection, tumor screening, and food safety testing; however, most PCR devices have inefficient heating and cooling ramp rates for the solution, whi...
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Autor*in:	Yi-Quan An [verfasserIn] Shao-Lei Huang [verfasserIn] Bang-Chao Xi [verfasserIn] Xiang-Lian Gong [verfasserIn] Jun-Hao Ji [verfasserIn] You Hu [verfasserIn] Yi-Jie Ding [verfasserIn] Dong-Xu Zhang [verfasserIn] Sheng-Xiang Ge [verfasserIn] Jun Zhang [verfasserIn] Ning-Shao Xia [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	polymerase chain reaction thermocycler heat transfer modeling analysis temperature overshoots fast heating and cooling ramp rates

Übergeordnetes Werk:	In: Micromachines - MDPI AG, 2010, 14(2023), 3, p 658
Übergeordnetes Werk:	volume:14 ; year:2023 ; number:3, p 658

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/mi14030658

Katalog-ID:	DOAJ087290588

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520			\|a The polymerase chain reaction (PCR) is essential in nucleic acid amplification tests and is widely used in many applications such as infectious disease detection, tumor screening, and food safety testing; however, most PCR devices have inefficient heating and cooling ramp rates for the solution, which significantly limit their application in special scenarios such as hospital emergencies, airports, and customs. Here, we propose a temperature control strategy to significantly increase the ramp rates for the solution temperature by switching microfluidic chips between multiple temperature zones and excessively increasing the temperature difference between temperature zones and the solution; accordingly, we have designed an ultrafast thermocycler. The results showed that the ramp rates of the solution temperature are a linear function of temperature differences within a range, and a larger temperature difference would result in faster ramp rates. The maximum heating and cooling ramp rates of the 25 μL solution reached 24.12 °C/s and 25.28 °C/s, respectively, and the average ramp rate was 13.33 °C/s, 6–8 times higher than that of conventional commercial PCR devices. The thermocycler achieved 9 min (1 min pre-denaturation + 45 PCR cycles) ultrafast nucleic acid amplification, shortening the time by 92% compared to the conventional 120 min nucleic acid amplification, and has the potential to be used for rapid nucleic acid detection.
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650		4	\|a temperature overshoots
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spelling	10.3390/mi14030658 doi (DE-627)DOAJ087290588 (DE-599)DOAJd56ef67a988a44b7816d49d5e9c2c6b4 DE-627 ger DE-627 rakwb eng TJ1-1570 Yi-Quan An verfasserin aut Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The polymerase chain reaction (PCR) is essential in nucleic acid amplification tests and is widely used in many applications such as infectious disease detection, tumor screening, and food safety testing; however, most PCR devices have inefficient heating and cooling ramp rates for the solution, which significantly limit their application in special scenarios such as hospital emergencies, airports, and customs. Here, we propose a temperature control strategy to significantly increase the ramp rates for the solution temperature by switching microfluidic chips between multiple temperature zones and excessively increasing the temperature difference between temperature zones and the solution; accordingly, we have designed an ultrafast thermocycler. The results showed that the ramp rates of the solution temperature are a linear function of temperature differences within a range, and a larger temperature difference would result in faster ramp rates. The maximum heating and cooling ramp rates of the 25 μL solution reached 24.12 °C/s and 25.28 °C/s, respectively, and the average ramp rate was 13.33 °C/s, 6–8 times higher than that of conventional commercial PCR devices. The thermocycler achieved 9 min (1 min pre-denaturation + 45 PCR cycles) ultrafast nucleic acid amplification, shortening the time by 92% compared to the conventional 120 min nucleic acid amplification, and has the potential to be used for rapid nucleic acid detection. polymerase chain reaction thermocycler heat transfer modeling analysis temperature overshoots fast heating and cooling ramp rates Mechanical engineering and machinery Shao-Lei Huang verfasserin aut Bang-Chao Xi verfasserin aut Xiang-Lian Gong verfasserin aut Jun-Hao Ji verfasserin aut You Hu verfasserin aut Yi-Jie Ding verfasserin aut Dong-Xu Zhang verfasserin aut Sheng-Xiang Ge verfasserin aut Jun Zhang verfasserin aut Ning-Shao Xia verfasserin aut In Micromachines MDPI AG, 2010 14(2023), 3, p 658 (DE-627)665016069 (DE-600)2620864-7 2072666X nnns volume:14 year:2023 number:3, p 658 https://doi.org/10.3390/mi14030658 kostenfrei https://doaj.org/article/d56ef67a988a44b7816d49d5e9c2c6b4 kostenfrei https://www.mdpi.com/2072-666X/14/3/658 kostenfrei https://doaj.org/toc/2072-666X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023 3, p 658
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allfieldsSound	10.3390/mi14030658 doi (DE-627)DOAJ087290588 (DE-599)DOAJd56ef67a988a44b7816d49d5e9c2c6b4 DE-627 ger DE-627 rakwb eng TJ1-1570 Yi-Quan An verfasserin aut Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The polymerase chain reaction (PCR) is essential in nucleic acid amplification tests and is widely used in many applications such as infectious disease detection, tumor screening, and food safety testing; however, most PCR devices have inefficient heating and cooling ramp rates for the solution, which significantly limit their application in special scenarios such as hospital emergencies, airports, and customs. Here, we propose a temperature control strategy to significantly increase the ramp rates for the solution temperature by switching microfluidic chips between multiple temperature zones and excessively increasing the temperature difference between temperature zones and the solution; accordingly, we have designed an ultrafast thermocycler. The results showed that the ramp rates of the solution temperature are a linear function of temperature differences within a range, and a larger temperature difference would result in faster ramp rates. The maximum heating and cooling ramp rates of the 25 μL solution reached 24.12 °C/s and 25.28 °C/s, respectively, and the average ramp rate was 13.33 °C/s, 6–8 times higher than that of conventional commercial PCR devices. The thermocycler achieved 9 min (1 min pre-denaturation + 45 PCR cycles) ultrafast nucleic acid amplification, shortening the time by 92% compared to the conventional 120 min nucleic acid amplification, and has the potential to be used for rapid nucleic acid detection. polymerase chain reaction thermocycler heat transfer modeling analysis temperature overshoots fast heating and cooling ramp rates Mechanical engineering and machinery Shao-Lei Huang verfasserin aut Bang-Chao Xi verfasserin aut Xiang-Lian Gong verfasserin aut Jun-Hao Ji verfasserin aut You Hu verfasserin aut Yi-Jie Ding verfasserin aut Dong-Xu Zhang verfasserin aut Sheng-Xiang Ge verfasserin aut Jun Zhang verfasserin aut Ning-Shao Xia verfasserin aut In Micromachines MDPI AG, 2010 14(2023), 3, p 658 (DE-627)665016069 (DE-600)2620864-7 2072666X nnns volume:14 year:2023 number:3, p 658 https://doi.org/10.3390/mi14030658 kostenfrei https://doaj.org/article/d56ef67a988a44b7816d49d5e9c2c6b4 kostenfrei https://www.mdpi.com/2072-666X/14/3/658 kostenfrei https://doaj.org/toc/2072-666X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023 3, p 658
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callnumber-first	T - Technology
author	Yi-Quan An
spellingShingle	Yi-Quan An misc TJ1-1570 misc polymerase chain reaction misc thermocycler misc heat transfer modeling analysis misc temperature overshoots misc fast heating and cooling ramp rates misc Mechanical engineering and machinery Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification
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topic_title	TJ1-1570 Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification polymerase chain reaction thermocycler heat transfer modeling analysis temperature overshoots fast heating and cooling ramp rates
topic	misc TJ1-1570 misc polymerase chain reaction misc thermocycler misc heat transfer modeling analysis misc temperature overshoots misc fast heating and cooling ramp rates misc Mechanical engineering and machinery
topic_unstemmed	misc TJ1-1570 misc polymerase chain reaction misc thermocycler misc heat transfer modeling analysis misc temperature overshoots misc fast heating and cooling ramp rates misc Mechanical engineering and machinery
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title	Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification
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title_full	Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification
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title_sort	ultrafast microfluidic pcr thermocycler for nucleic acid amplification
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title_auth	Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification
abstract	The polymerase chain reaction (PCR) is essential in nucleic acid amplification tests and is widely used in many applications such as infectious disease detection, tumor screening, and food safety testing; however, most PCR devices have inefficient heating and cooling ramp rates for the solution, which significantly limit their application in special scenarios such as hospital emergencies, airports, and customs. Here, we propose a temperature control strategy to significantly increase the ramp rates for the solution temperature by switching microfluidic chips between multiple temperature zones and excessively increasing the temperature difference between temperature zones and the solution; accordingly, we have designed an ultrafast thermocycler. The results showed that the ramp rates of the solution temperature are a linear function of temperature differences within a range, and a larger temperature difference would result in faster ramp rates. The maximum heating and cooling ramp rates of the 25 μL solution reached 24.12 °C/s and 25.28 °C/s, respectively, and the average ramp rate was 13.33 °C/s, 6–8 times higher than that of conventional commercial PCR devices. The thermocycler achieved 9 min (1 min pre-denaturation + 45 PCR cycles) ultrafast nucleic acid amplification, shortening the time by 92% compared to the conventional 120 min nucleic acid amplification, and has the potential to be used for rapid nucleic acid detection.
abstractGer	The polymerase chain reaction (PCR) is essential in nucleic acid amplification tests and is widely used in many applications such as infectious disease detection, tumor screening, and food safety testing; however, most PCR devices have inefficient heating and cooling ramp rates for the solution, which significantly limit their application in special scenarios such as hospital emergencies, airports, and customs. Here, we propose a temperature control strategy to significantly increase the ramp rates for the solution temperature by switching microfluidic chips between multiple temperature zones and excessively increasing the temperature difference between temperature zones and the solution; accordingly, we have designed an ultrafast thermocycler. The results showed that the ramp rates of the solution temperature are a linear function of temperature differences within a range, and a larger temperature difference would result in faster ramp rates. The maximum heating and cooling ramp rates of the 25 μL solution reached 24.12 °C/s and 25.28 °C/s, respectively, and the average ramp rate was 13.33 °C/s, 6–8 times higher than that of conventional commercial PCR devices. The thermocycler achieved 9 min (1 min pre-denaturation + 45 PCR cycles) ultrafast nucleic acid amplification, shortening the time by 92% compared to the conventional 120 min nucleic acid amplification, and has the potential to be used for rapid nucleic acid detection.
abstract_unstemmed	The polymerase chain reaction (PCR) is essential in nucleic acid amplification tests and is widely used in many applications such as infectious disease detection, tumor screening, and food safety testing; however, most PCR devices have inefficient heating and cooling ramp rates for the solution, which significantly limit their application in special scenarios such as hospital emergencies, airports, and customs. Here, we propose a temperature control strategy to significantly increase the ramp rates for the solution temperature by switching microfluidic chips between multiple temperature zones and excessively increasing the temperature difference between temperature zones and the solution; accordingly, we have designed an ultrafast thermocycler. The results showed that the ramp rates of the solution temperature are a linear function of temperature differences within a range, and a larger temperature difference would result in faster ramp rates. The maximum heating and cooling ramp rates of the 25 μL solution reached 24.12 °C/s and 25.28 °C/s, respectively, and the average ramp rate was 13.33 °C/s, 6–8 times higher than that of conventional commercial PCR devices. The thermocycler achieved 9 min (1 min pre-denaturation + 45 PCR cycles) ultrafast nucleic acid amplification, shortening the time by 92% compared to the conventional 120 min nucleic acid amplification, and has the potential to be used for rapid nucleic acid detection.
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title_short	Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification
url	https://doi.org/10.3390/mi14030658 https://doaj.org/article/d56ef67a988a44b7816d49d5e9c2c6b4 https://www.mdpi.com/2072-666X/14/3/658 https://doaj.org/toc/2072-666X
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up_date	2024-07-04T01:03:37.876Z
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Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification

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