Shared Genes of <i<PPARG</i< and <i<NOS2</i< in Alzheimer’s Disease and Ulcerative Colitis Drive Macrophages and Microglia Polarization: Evidence from Bioinformatics Analysis and Following Validation

Emerging evidence shows that peripheral systemic inflammation, such as inflammatory bowel disease (IBD), has a close even interaction with central nervous disorders such as Alzheimer’s disease (AD). This study is designed to further clarify the relationship between AD and ulcerative colitis (UC, a s...
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Autor*in:	Longcong Dong [verfasserIn] Yuan Shen [verfasserIn] Hongying Li [verfasserIn] Ruibin Zhang [verfasserIn] Shuguang Yu [verfasserIn] Qiaofeng Wu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	Alzheimer’s disease ulcerative colitis shared gene bioinformatics

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 24(2023), 6, p 5651
Übergeordnetes Werk:	volume:24 ; year:2023 ; number:6, p 5651

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms24065651

Katalog-ID:	DOAJ087339536

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callnumber-first	Q - Science
author	Longcong Dong
spellingShingle	Longcong Dong misc QH301-705.5 misc QD1-999 misc Alzheimer’s disease misc ulcerative colitis misc <i<PPARG</i< misc <i<NOS2</i< misc shared gene misc bioinformatics misc Biology (General) misc Chemistry Shared Genes of <i<PPARG</i< and <i<NOS2</i< in Alzheimer’s Disease and Ulcerative Colitis Drive Macrophages and Microglia Polarization: Evidence from Bioinformatics Analysis and Following Validation
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topic_title	QH301-705.5 QD1-999 Shared Genes of <i<PPARG</i< and <i<NOS2</i< in Alzheimer’s Disease and Ulcerative Colitis Drive Macrophages and Microglia Polarization: Evidence from Bioinformatics Analysis and Following Validation Alzheimer’s disease ulcerative colitis <i<PPARG</i< <i<NOS2</i< shared gene bioinformatics
topic	misc QH301-705.5 misc QD1-999 misc Alzheimer’s disease misc ulcerative colitis misc <i<PPARG</i< misc <i<NOS2</i< misc shared gene misc bioinformatics misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc Alzheimer’s disease misc ulcerative colitis misc <i<PPARG</i< misc <i<NOS2</i< misc shared gene misc bioinformatics misc Biology (General) misc Chemistry
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title	Shared Genes of <i<PPARG</i< and <i<NOS2</i< in Alzheimer’s Disease and Ulcerative Colitis Drive Macrophages and Microglia Polarization: Evidence from Bioinformatics Analysis and Following Validation
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title_full	Shared Genes of <i<PPARG</i< and <i<NOS2</i< in Alzheimer’s Disease and Ulcerative Colitis Drive Macrophages and Microglia Polarization: Evidence from Bioinformatics Analysis and Following Validation
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author_browse	Longcong Dong Yuan Shen Hongying Li Ruibin Zhang Shuguang Yu Qiaofeng Wu
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title_sort	shared genes of <i<pparg</i< and <i<nos2</i< in alzheimer’s disease and ulcerative colitis drive macrophages and microglia polarization: evidence from bioinformatics analysis and following validation
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title_auth	Shared Genes of <i<PPARG</i< and <i<NOS2</i< in Alzheimer’s Disease and Ulcerative Colitis Drive Macrophages and Microglia Polarization: Evidence from Bioinformatics Analysis and Following Validation
abstract	Emerging evidence shows that peripheral systemic inflammation, such as inflammatory bowel disease (IBD), has a close even interaction with central nervous disorders such as Alzheimer’s disease (AD). This study is designed to further clarify the relationship between AD and ulcerative colitis (UC, a subclass of IBD). The GEO database was used to download gene expression profiles for AD (GSE5281) and UC (GSE47908). Bioinformatics analysis included GSEA, KEGG pathway, Gene Ontology (GO), WikiPathways, PPI network, and hub gene identification. After screening the shared genes, qRT-PCR, Western blot, and immunofluorescence were used to verify the reliability of the dataset and further confirm the shared genes. GSEA, KEGG, GO, and WikiPathways suggested that <i<PPARG</i< and <i<NOS2</i< were identified as shared genes and hub genes by cytoHubba in AD and UC and further validated via qRT-PCR and Western blot. Our work identified <i<PPARG</i< and <i<NOS2</i< are shared genes of AD and UC. They drive macrophages and microglia heterogeneous polarization, which may be potential targets for treating neural dysfunction induced by systemic inflammation and vice versa.
abstractGer	Emerging evidence shows that peripheral systemic inflammation, such as inflammatory bowel disease (IBD), has a close even interaction with central nervous disorders such as Alzheimer’s disease (AD). This study is designed to further clarify the relationship between AD and ulcerative colitis (UC, a subclass of IBD). The GEO database was used to download gene expression profiles for AD (GSE5281) and UC (GSE47908). Bioinformatics analysis included GSEA, KEGG pathway, Gene Ontology (GO), WikiPathways, PPI network, and hub gene identification. After screening the shared genes, qRT-PCR, Western blot, and immunofluorescence were used to verify the reliability of the dataset and further confirm the shared genes. GSEA, KEGG, GO, and WikiPathways suggested that <i<PPARG</i< and <i<NOS2</i< were identified as shared genes and hub genes by cytoHubba in AD and UC and further validated via qRT-PCR and Western blot. Our work identified <i<PPARG</i< and <i<NOS2</i< are shared genes of AD and UC. They drive macrophages and microglia heterogeneous polarization, which may be potential targets for treating neural dysfunction induced by systemic inflammation and vice versa.
abstract_unstemmed	Emerging evidence shows that peripheral systemic inflammation, such as inflammatory bowel disease (IBD), has a close even interaction with central nervous disorders such as Alzheimer’s disease (AD). This study is designed to further clarify the relationship between AD and ulcerative colitis (UC, a subclass of IBD). The GEO database was used to download gene expression profiles for AD (GSE5281) and UC (GSE47908). Bioinformatics analysis included GSEA, KEGG pathway, Gene Ontology (GO), WikiPathways, PPI network, and hub gene identification. After screening the shared genes, qRT-PCR, Western blot, and immunofluorescence were used to verify the reliability of the dataset and further confirm the shared genes. GSEA, KEGG, GO, and WikiPathways suggested that <i<PPARG</i< and <i<NOS2</i< were identified as shared genes and hub genes by cytoHubba in AD and UC and further validated via qRT-PCR and Western blot. Our work identified <i<PPARG</i< and <i<NOS2</i< are shared genes of AD and UC. They drive macrophages and microglia heterogeneous polarization, which may be potential targets for treating neural dysfunction induced by systemic inflammation and vice versa.
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title_short	Shared Genes of <i<PPARG</i< and <i<NOS2</i< in Alzheimer’s Disease and Ulcerative Colitis Drive Macrophages and Microglia Polarization: Evidence from Bioinformatics Analysis and Following Validation
url	https://doi.org/10.3390/ijms24065651 https://doaj.org/article/4cb6e89f3ff34d6e85abadd99efca30c https://www.mdpi.com/1422-0067/24/6/5651 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
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author2	Yuan Shen Hongying Li Ruibin Zhang Shuguang Yu Qiaofeng Wu
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up_date	2024-07-04T01:16:23.522Z
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This study is designed to further clarify the relationship between AD and ulcerative colitis (UC, a subclass of IBD). The GEO database was used to download gene expression profiles for AD (GSE5281) and UC (GSE47908). Bioinformatics analysis included GSEA, KEGG pathway, Gene Ontology (GO), WikiPathways, PPI network, and hub gene identification. After screening the shared genes, qRT-PCR, Western blot, and immunofluorescence were used to verify the reliability of the dataset and further confirm the shared genes. GSEA, KEGG, GO, and WikiPathways suggested that <i<PPARG</i< and <i<NOS2</i< were identified as shared genes and hub genes by cytoHubba in AD and UC and further validated via qRT-PCR and Western blot. Our work identified <i<PPARG</i< and <i<NOS2</i< are shared genes of AD and UC. 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Shared Genes of <i<PPARG</i< and <i<NOS2</i< in Alzheimer’s Disease and Ulcerative Colitis Drive Macrophages and Microglia Polarization: Evidence from Bioinformatics Analysis and Following Validation

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