Growth Conditions Influence <i<Lactobacillus</i< Cell-Free Supernatant Impact on Viability, Biofilm Formation, and Co-Aggregation of the Oral Periodontopathogens <i<Fusobacterium nucleatum</i< and <i<Porphyromonas gingivalis</i<

<i<Fusobacterium nucleatum</i< and <i<Porphyromonas gingivalis</i< human periodontopathogens play a leading part in oral squamous cell carcinoma through cell proliferation, invasion, and persistent inflammation promotion and maintenance. To explore how the activity of <i&l...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Paola Zanetta [verfasserIn] Diletta Francesca Squarzanti [verfasserIn] Alessia di Coste [verfasserIn] Angela Amoruso [verfasserIn] Marco Pane [verfasserIn] Barbara Azzimonti [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	periodontopathogens probiotics probiotic cell-free supernatant

Übergeordnetes Werk:	In: Biomedicines - MDPI AG, 2014, 11(2023), 3, p 859
Übergeordnetes Werk:	volume:11 ; year:2023 ; number:3, p 859

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/biomedicines11030859

Katalog-ID:	DOAJ087428644

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allfields	10.3390/biomedicines11030859 doi (DE-627)DOAJ087428644 (DE-599)DOAJ2c72e760a72d4a7c9f3321d8daf50212 DE-627 ger DE-627 rakwb eng QH301-705.5 Paola Zanetta verfasserin aut Growth Conditions Influence <i<Lactobacillus</i< Cell-Free Supernatant Impact on Viability, Biofilm Formation, and Co-Aggregation of the Oral Periodontopathogens <i<Fusobacterium nucleatum</i< and <i<Porphyromonas gingivalis</i< 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Fusobacterium nucleatum</i< and <i<Porphyromonas gingivalis</i< human periodontopathogens play a leading part in oral squamous cell carcinoma through cell proliferation, invasion, and persistent inflammation promotion and maintenance. To explore how the activity of <i<Lactobacillus</i<-derived cell-free supernatants (CFSs) can be influenced by growth medium components, CFSs were produced both in the standard MRS and the novel animal-derivative-free “Terreno Industriale Lattobacilli” (TIL) media, and in vitro screened for the containment of <i<F. nucleatum</i< and <i<P. gingivalis</i< both single and co-cultured and also for the interference on their co-aggregation. The viability assay demonstrated that the <i<Limosilactobacillus reuteri</i< LRE11 and <i<Ligilactobacillus salivarius</i< LS03 MRS-produced CFSs were significantly more effective against single and co-cultured pathogens. All the other CFSs significantly improved their efficacy when produced in TIL. Both MRS- and TIL-produced CFSs significantly inhibited the single and co-cultured pathogen biofilm formation. Only <i<Levilactobacillus brevis</i< LBR01 CFS in MRS specifically reduced <i<F. nucleatum</i< and <i<P. gingivalis</i< co-aggregation, while viable LBR01, LS03, and LRE11 in MRS significantly co-aggregated with the pathogens, but only LS03 cultivated in TIL improved this effect. This work paves the way to better consider environmental growth conditions when screening for probiotic and postbiotic efficacy as crucial to pathogen aggregation, adhesion to the host’s niches, and exclusion. <i<Fusobacterium nucleatum</i< <i<Porphyromonas gingivalis</i< periodontopathogens <i<Lactobacillus</i< probiotics probiotic cell-free supernatant Biology (General) Diletta Francesca Squarzanti verfasserin aut Alessia di Coste verfasserin aut Angela Amoruso verfasserin aut Marco Pane verfasserin aut Barbara Azzimonti verfasserin aut In Biomedicines MDPI AG, 2014 11(2023), 3, p 859 (DE-627)750370483 (DE-600)2720867-9 22279059 nnns volume:11 year:2023 number:3, p 859 https://doi.org/10.3390/biomedicines11030859 kostenfrei https://doaj.org/article/2c72e760a72d4a7c9f3321d8daf50212 kostenfrei https://www.mdpi.com/2227-9059/11/3/859 kostenfrei https://doaj.org/toc/2227-9059 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2023 3, p 859
spelling	10.3390/biomedicines11030859 doi (DE-627)DOAJ087428644 (DE-599)DOAJ2c72e760a72d4a7c9f3321d8daf50212 DE-627 ger DE-627 rakwb eng QH301-705.5 Paola Zanetta verfasserin aut Growth Conditions Influence <i<Lactobacillus</i< Cell-Free Supernatant Impact on Viability, Biofilm Formation, and Co-Aggregation of the Oral Periodontopathogens <i<Fusobacterium nucleatum</i< and <i<Porphyromonas gingivalis</i< 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Fusobacterium nucleatum</i< and <i<Porphyromonas gingivalis</i< human periodontopathogens play a leading part in oral squamous cell carcinoma through cell proliferation, invasion, and persistent inflammation promotion and maintenance. To explore how the activity of <i<Lactobacillus</i<-derived cell-free supernatants (CFSs) can be influenced by growth medium components, CFSs were produced both in the standard MRS and the novel animal-derivative-free “Terreno Industriale Lattobacilli” (TIL) media, and in vitro screened for the containment of <i<F. nucleatum</i< and <i<P. gingivalis</i< both single and co-cultured and also for the interference on their co-aggregation. The viability assay demonstrated that the <i<Limosilactobacillus reuteri</i< LRE11 and <i<Ligilactobacillus salivarius</i< LS03 MRS-produced CFSs were significantly more effective against single and co-cultured pathogens. All the other CFSs significantly improved their efficacy when produced in TIL. Both MRS- and TIL-produced CFSs significantly inhibited the single and co-cultured pathogen biofilm formation. Only <i<Levilactobacillus brevis</i< LBR01 CFS in MRS specifically reduced <i<F. nucleatum</i< and <i<P. gingivalis</i< co-aggregation, while viable LBR01, LS03, and LRE11 in MRS significantly co-aggregated with the pathogens, but only LS03 cultivated in TIL improved this effect. This work paves the way to better consider environmental growth conditions when screening for probiotic and postbiotic efficacy as crucial to pathogen aggregation, adhesion to the host’s niches, and exclusion. <i<Fusobacterium nucleatum</i< <i<Porphyromonas gingivalis</i< periodontopathogens <i<Lactobacillus</i< probiotics probiotic cell-free supernatant Biology (General) Diletta Francesca Squarzanti verfasserin aut Alessia di Coste verfasserin aut Angela Amoruso verfasserin aut Marco Pane verfasserin aut Barbara Azzimonti verfasserin aut In Biomedicines MDPI AG, 2014 11(2023), 3, p 859 (DE-627)750370483 (DE-600)2720867-9 22279059 nnns volume:11 year:2023 number:3, p 859 https://doi.org/10.3390/biomedicines11030859 kostenfrei https://doaj.org/article/2c72e760a72d4a7c9f3321d8daf50212 kostenfrei https://www.mdpi.com/2227-9059/11/3/859 kostenfrei https://doaj.org/toc/2227-9059 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2023 3, p 859
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topic_title	QH301-705.5 Growth Conditions Influence <i<Lactobacillus</i< Cell-Free Supernatant Impact on Viability, Biofilm Formation, and Co-Aggregation of the Oral Periodontopathogens <i<Fusobacterium nucleatum</i< and <i<Porphyromonas gingivalis</i< <i<Fusobacterium nucleatum</i< <i<Porphyromonas gingivalis</i< periodontopathogens <i<Lactobacillus</i< probiotics probiotic cell-free supernatant
topic	misc QH301-705.5 misc <i<Fusobacterium nucleatum</i< misc <i<Porphyromonas gingivalis</i< misc periodontopathogens misc <i<Lactobacillus</i< misc probiotics misc probiotic cell-free supernatant misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc <i<Fusobacterium nucleatum</i< misc <i<Porphyromonas gingivalis</i< misc periodontopathogens misc <i<Lactobacillus</i< misc probiotics misc probiotic cell-free supernatant misc Biology (General)
topic_browse	misc QH301-705.5 misc <i<Fusobacterium nucleatum</i< misc <i<Porphyromonas gingivalis</i< misc periodontopathogens misc <i<Lactobacillus</i< misc probiotics misc probiotic cell-free supernatant misc Biology (General)
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title	Growth Conditions Influence <i<Lactobacillus</i< Cell-Free Supernatant Impact on Viability, Biofilm Formation, and Co-Aggregation of the Oral Periodontopathogens <i<Fusobacterium nucleatum</i< and <i<Porphyromonas gingivalis</i<
ctrlnum	(DE-627)DOAJ087428644 (DE-599)DOAJ2c72e760a72d4a7c9f3321d8daf50212
title_full	Growth Conditions Influence <i<Lactobacillus</i< Cell-Free Supernatant Impact on Viability, Biofilm Formation, and Co-Aggregation of the Oral Periodontopathogens <i<Fusobacterium nucleatum</i< and <i<Porphyromonas gingivalis</i<
author_sort	Paola Zanetta
journal	Biomedicines
journalStr	Biomedicines
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author_browse	Paola Zanetta Diletta Francesca Squarzanti Alessia di Coste Angela Amoruso Marco Pane Barbara Azzimonti
container_volume	11
class	QH301-705.5
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author-letter	Paola Zanetta
doi_str_mv	10.3390/biomedicines11030859
author2-role	verfasserin
title_sort	growth conditions influence <i<lactobacillus</i< cell-free supernatant impact on viability, biofilm formation, and co-aggregation of the oral periodontopathogens <i<fusobacterium nucleatum</i< and <i<porphyromonas gingivalis</i<
callnumber	QH301-705.5
title_auth	Growth Conditions Influence <i<Lactobacillus</i< Cell-Free Supernatant Impact on Viability, Biofilm Formation, and Co-Aggregation of the Oral Periodontopathogens <i<Fusobacterium nucleatum</i< and <i<Porphyromonas gingivalis</i<
abstract	<i<Fusobacterium nucleatum</i< and <i<Porphyromonas gingivalis</i< human periodontopathogens play a leading part in oral squamous cell carcinoma through cell proliferation, invasion, and persistent inflammation promotion and maintenance. To explore how the activity of <i<Lactobacillus</i<-derived cell-free supernatants (CFSs) can be influenced by growth medium components, CFSs were produced both in the standard MRS and the novel animal-derivative-free “Terreno Industriale Lattobacilli” (TIL) media, and in vitro screened for the containment of <i<F. nucleatum</i< and <i<P. gingivalis</i< both single and co-cultured and also for the interference on their co-aggregation. The viability assay demonstrated that the <i<Limosilactobacillus reuteri</i< LRE11 and <i<Ligilactobacillus salivarius</i< LS03 MRS-produced CFSs were significantly more effective against single and co-cultured pathogens. All the other CFSs significantly improved their efficacy when produced in TIL. Both MRS- and TIL-produced CFSs significantly inhibited the single and co-cultured pathogen biofilm formation. Only <i<Levilactobacillus brevis</i< LBR01 CFS in MRS specifically reduced <i<F. nucleatum</i< and <i<P. gingivalis</i< co-aggregation, while viable LBR01, LS03, and LRE11 in MRS significantly co-aggregated with the pathogens, but only LS03 cultivated in TIL improved this effect. This work paves the way to better consider environmental growth conditions when screening for probiotic and postbiotic efficacy as crucial to pathogen aggregation, adhesion to the host’s niches, and exclusion.
abstractGer	<i<Fusobacterium nucleatum</i< and <i<Porphyromonas gingivalis</i< human periodontopathogens play a leading part in oral squamous cell carcinoma through cell proliferation, invasion, and persistent inflammation promotion and maintenance. To explore how the activity of <i<Lactobacillus</i<-derived cell-free supernatants (CFSs) can be influenced by growth medium components, CFSs were produced both in the standard MRS and the novel animal-derivative-free “Terreno Industriale Lattobacilli” (TIL) media, and in vitro screened for the containment of <i<F. nucleatum</i< and <i<P. gingivalis</i< both single and co-cultured and also for the interference on their co-aggregation. The viability assay demonstrated that the <i<Limosilactobacillus reuteri</i< LRE11 and <i<Ligilactobacillus salivarius</i< LS03 MRS-produced CFSs were significantly more effective against single and co-cultured pathogens. All the other CFSs significantly improved their efficacy when produced in TIL. Both MRS- and TIL-produced CFSs significantly inhibited the single and co-cultured pathogen biofilm formation. Only <i<Levilactobacillus brevis</i< LBR01 CFS in MRS specifically reduced <i<F. nucleatum</i< and <i<P. gingivalis</i< co-aggregation, while viable LBR01, LS03, and LRE11 in MRS significantly co-aggregated with the pathogens, but only LS03 cultivated in TIL improved this effect. This work paves the way to better consider environmental growth conditions when screening for probiotic and postbiotic efficacy as crucial to pathogen aggregation, adhesion to the host’s niches, and exclusion.
abstract_unstemmed	<i<Fusobacterium nucleatum</i< and <i<Porphyromonas gingivalis</i< human periodontopathogens play a leading part in oral squamous cell carcinoma through cell proliferation, invasion, and persistent inflammation promotion and maintenance. To explore how the activity of <i<Lactobacillus</i<-derived cell-free supernatants (CFSs) can be influenced by growth medium components, CFSs were produced both in the standard MRS and the novel animal-derivative-free “Terreno Industriale Lattobacilli” (TIL) media, and in vitro screened for the containment of <i<F. nucleatum</i< and <i<P. gingivalis</i< both single and co-cultured and also for the interference on their co-aggregation. The viability assay demonstrated that the <i<Limosilactobacillus reuteri</i< LRE11 and <i<Ligilactobacillus salivarius</i< LS03 MRS-produced CFSs were significantly more effective against single and co-cultured pathogens. All the other CFSs significantly improved their efficacy when produced in TIL. Both MRS- and TIL-produced CFSs significantly inhibited the single and co-cultured pathogen biofilm formation. Only <i<Levilactobacillus brevis</i< LBR01 CFS in MRS specifically reduced <i<F. nucleatum</i< and <i<P. gingivalis</i< co-aggregation, while viable LBR01, LS03, and LRE11 in MRS significantly co-aggregated with the pathogens, but only LS03 cultivated in TIL improved this effect. This work paves the way to better consider environmental growth conditions when screening for probiotic and postbiotic efficacy as crucial to pathogen aggregation, adhesion to the host’s niches, and exclusion.
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container_issue	3, p 859
title_short	Growth Conditions Influence <i<Lactobacillus</i< Cell-Free Supernatant Impact on Viability, Biofilm Formation, and Co-Aggregation of the Oral Periodontopathogens <i<Fusobacterium nucleatum</i< and <i<Porphyromonas gingivalis</i<
url	https://doi.org/10.3390/biomedicines11030859 https://doaj.org/article/2c72e760a72d4a7c9f3321d8daf50212 https://www.mdpi.com/2227-9059/11/3/859 https://doaj.org/toc/2227-9059
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author2	Diletta Francesca Squarzanti Alessia di Coste Angela Amoruso Marco Pane Barbara Azzimonti
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up_date	2024-07-04T01:39:06.133Z
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