Immune and metabolic markers for identifying and investigating severe Coronavirus disease and Sepsis in children and young people (pSeP/COVID ChYP study): protocol for a prospective cohort study
Introduction Early recognition and appropriate management of paediatric sepsis are known to improve outcomes. A previous system’s biology investigation of the systemic immune response in neonates to sepsis identified immune and metabolic markers that showed high accuracy for detecting bacterial infe...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Richard Skone [verfasserIn] Daniel White [verfasserIn] Bethan Phillips [verfasserIn] Jennifer Evans [verfasserIn] Robert Andrews [verfasserIn] Benjamin Saunders [verfasserIn] Kerry Hood [verfasserIn] Mallinath Chakraborty [verfasserIn] Sarah Edkins [verfasserIn] Barbara Paquete [verfasserIn] Sian Foulkes [verfasserIn] Anna Barrow [verfasserIn] Siske Struik [verfasserIn] Valerie O'Donnell [verfasserIn] Sara Ali [verfasserIn] Patrícia R S Rodrigues [verfasserIn] Angela Strang [verfasserIn] Summia Zaher [verfasserIn] Simran Sharma [verfasserIn] Luke C Davies [verfasserIn] Linda Moet [verfasserIn] James E McLaren [verfasserIn] Gareth L Watson [verfasserIn] Peter Ghazal [verfasserIn] Edward Parkinson [verfasserIn] Sivakumar Oruganti [verfasserIn] William John Watkins [verfasserIn] Selyf Shapey [verfasserIn] Rim al Samsam [verfasserIn] Malcolm Gajraj [verfasserIn] Michelle Jardine [verfasserIn] Jong Eun Song [verfasserIn] Lloyd Abood [verfasserIn] Sarah Joanne Kotecha [verfasserIn] Awen Evans [verfasserIn] Iona Buchanan [verfasserIn] Susan Bowes [verfasserIn] Begum Ali [verfasserIn] Maya Gore [verfasserIn] Rhian Thomas-Turner [verfasserIn] Federico Liberatore [verfasserIn] Thomas Woolley [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2023 |
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| Übergeordnetes Werk: |
In: BMJ Open - BMJ Publishing Group, 2011, 13(2023), 3 |
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| Übergeordnetes Werk: |
volume:13 ; year:2023 ; number:3 |
| Links: |
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| DOI / URN: |
10.1136/bmjopen-2022-067002 |
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| Katalog-ID: |
DOAJ087493934 |
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| 245 | 1 | 0 | |a Immune and metabolic markers for identifying and investigating severe Coronavirus disease and Sepsis in children and young people (pSeP/COVID ChYP study): protocol for a prospective cohort study |
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| 520 | |a Introduction Early recognition and appropriate management of paediatric sepsis are known to improve outcomes. A previous system’s biology investigation of the systemic immune response in neonates to sepsis identified immune and metabolic markers that showed high accuracy for detecting bacterial infection. Further gene expression markers have also been reported previously in the paediatric age group for discriminating sepsis from control cases. More recently, specific gene signatures were identified to discriminate between COVID-19 and its associated inflammatory sequelae. Through the current prospective cohort study, we aim to evaluate immune and metabolic blood markers which discriminate between sepses (including COVID-19) from other acute illnesses in critically unwell children and young persons, up to 18 years of age.Methods and analysis We describe a prospective cohort study for comparing the immune and metabolic whole-blood markers in patients with sepsis, COVID-19 and other illnesses. Clinical phenotyping and blood culture test results will provide a reference standard to evaluate the performance of blood markers from the research sample analysis. Serial sampling of whole blood (50 μL each) will be collected from children admitted to intensive care and with an acute illness to follow time dependent changes in biomarkers. An integrated lipidomics and RNASeq transcriptomics analyses will be conducted to evaluate immune-metabolic networks that discriminate sepsis and COVID-19 from other acute illnesses. This study received approval for deferred consent.Ethics and dissemination The study has received research ethics committee approval from the Yorkshire and Humber Leeds West Research Ethics Committee 2 (reference 20/YH/0214; IRAS reference 250612). Submission of study results for publication will involve making available all anonymised primary and processed data on public repository sites.Trial registration number NCT04904523. | ||
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| 700 | 0 | |a Bethan Phillips |e verfasserin |4 aut | |
| 700 | 0 | |a Jennifer Evans |e verfasserin |4 aut | |
| 700 | 0 | |a Robert Andrews |e verfasserin |4 aut | |
| 700 | 0 | |a Benjamin Saunders |e verfasserin |4 aut | |
| 700 | 0 | |a Kerry Hood |e verfasserin |4 aut | |
| 700 | 0 | |a Mallinath Chakraborty |e verfasserin |4 aut | |
| 700 | 0 | |a Sarah Edkins |e verfasserin |4 aut | |
| 700 | 0 | |a Barbara Paquete |e verfasserin |4 aut | |
| 700 | 0 | |a Sian Foulkes |e verfasserin |4 aut | |
| 700 | 0 | |a Anna Barrow |e verfasserin |4 aut | |
| 700 | 0 | |a Siske Struik |e verfasserin |4 aut | |
| 700 | 0 | |a Valerie O'Donnell |e verfasserin |4 aut | |
| 700 | 0 | |a Sara Ali |e verfasserin |4 aut | |
| 700 | 0 | |a Patrícia R S Rodrigues |e verfasserin |4 aut | |
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| 700 | 0 | |a Luke C Davies |e verfasserin |4 aut | |
| 700 | 0 | |a Linda Moet |e verfasserin |4 aut | |
| 700 | 0 | |a James E McLaren |e verfasserin |4 aut | |
| 700 | 0 | |a Gareth L Watson |e verfasserin |4 aut | |
| 700 | 0 | |a Peter Ghazal |e verfasserin |4 aut | |
| 700 | 0 | |a Edward Parkinson |e verfasserin |4 aut | |
| 700 | 0 | |a Sivakumar Oruganti |e verfasserin |4 aut | |
| 700 | 0 | |a William John Watkins |e verfasserin |4 aut | |
| 700 | 0 | |a Selyf Shapey |e verfasserin |4 aut | |
| 700 | 0 | |a Rim al Samsam |e verfasserin |4 aut | |
| 700 | 0 | |a Malcolm Gajraj |e verfasserin |4 aut | |
| 700 | 0 | |a Michelle Jardine |e verfasserin |4 aut | |
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| 700 | 0 | |a Awen Evans |e verfasserin |4 aut | |
| 700 | 0 | |a Iona Buchanan |e verfasserin |4 aut | |
| 700 | 0 | |a Susan Bowes |e verfasserin |4 aut | |
| 700 | 0 | |a Begum Ali |e verfasserin |4 aut | |
| 700 | 0 | |a Maya Gore |e verfasserin |4 aut | |
| 700 | 0 | |a Rhian Thomas-Turner |e verfasserin |4 aut | |
| 700 | 0 | |a Federico Liberatore |e verfasserin |4 aut | |
| 700 | 0 | |a Thomas Woolley |e verfasserin |4 aut | |
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10.1136/bmjopen-2022-067002 doi (DE-627)DOAJ087493934 (DE-599)DOAJ2d1c8f29b48f4539bf38b44ed3678f38 DE-627 ger DE-627 rakwb eng Richard Skone verfasserin aut Immune and metabolic markers for identifying and investigating severe Coronavirus disease and Sepsis in children and young people (pSeP/COVID ChYP study): protocol for a prospective cohort study 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction Early recognition and appropriate management of paediatric sepsis are known to improve outcomes. A previous system’s biology investigation of the systemic immune response in neonates to sepsis identified immune and metabolic markers that showed high accuracy for detecting bacterial infection. Further gene expression markers have also been reported previously in the paediatric age group for discriminating sepsis from control cases. More recently, specific gene signatures were identified to discriminate between COVID-19 and its associated inflammatory sequelae. Through the current prospective cohort study, we aim to evaluate immune and metabolic blood markers which discriminate between sepses (including COVID-19) from other acute illnesses in critically unwell children and young persons, up to 18 years of age.Methods and analysis We describe a prospective cohort study for comparing the immune and metabolic whole-blood markers in patients with sepsis, COVID-19 and other illnesses. Clinical phenotyping and blood culture test results will provide a reference standard to evaluate the performance of blood markers from the research sample analysis. Serial sampling of whole blood (50 μL each) will be collected from children admitted to intensive care and with an acute illness to follow time dependent changes in biomarkers. An integrated lipidomics and RNASeq transcriptomics analyses will be conducted to evaluate immune-metabolic networks that discriminate sepsis and COVID-19 from other acute illnesses. This study received approval for deferred consent.Ethics and dissemination The study has received research ethics committee approval from the Yorkshire and Humber Leeds West Research Ethics Committee 2 (reference 20/YH/0214; IRAS reference 250612). Submission of study results for publication will involve making available all anonymised primary and processed data on public repository sites.Trial registration number NCT04904523. Medicine R Daniel White verfasserin aut Bethan Phillips verfasserin aut Jennifer Evans verfasserin aut Robert Andrews verfasserin aut Benjamin Saunders verfasserin aut Kerry Hood verfasserin aut Mallinath Chakraborty verfasserin aut Sarah Edkins verfasserin aut Barbara Paquete verfasserin aut Sian Foulkes verfasserin aut Anna Barrow verfasserin aut Siske Struik verfasserin aut Valerie O'Donnell verfasserin aut Sara Ali verfasserin aut Patrícia R S Rodrigues verfasserin aut Angela Strang verfasserin aut Summia Zaher verfasserin aut Simran Sharma verfasserin aut Luke C Davies verfasserin aut Linda Moet verfasserin aut James E McLaren verfasserin aut Gareth L Watson verfasserin aut Peter Ghazal verfasserin aut Edward Parkinson verfasserin aut Sivakumar Oruganti verfasserin aut William John Watkins verfasserin aut Selyf Shapey verfasserin aut Rim al Samsam verfasserin aut Malcolm Gajraj verfasserin aut Michelle Jardine verfasserin aut Jong Eun Song verfasserin aut Lloyd Abood verfasserin aut Sarah Joanne Kotecha verfasserin aut Awen Evans verfasserin aut Iona Buchanan verfasserin aut Susan Bowes verfasserin aut Begum Ali verfasserin aut Maya Gore verfasserin aut Rhian Thomas-Turner verfasserin aut Federico Liberatore verfasserin aut Thomas Woolley verfasserin aut In BMJ Open BMJ Publishing Group, 2011 13(2023), 3 (DE-627)654747075 (DE-600)2599832-8 20446055 nnns volume:13 year:2023 number:3 https://doi.org/10.1136/bmjopen-2022-067002 kostenfrei https://doaj.org/article/2d1c8f29b48f4539bf38b44ed3678f38 kostenfrei https://bmjopen.bmj.com/content/13/3/e067002.full kostenfrei https://doaj.org/toc/2044-6055 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_375 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 3 |
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10.1136/bmjopen-2022-067002 doi (DE-627)DOAJ087493934 (DE-599)DOAJ2d1c8f29b48f4539bf38b44ed3678f38 DE-627 ger DE-627 rakwb eng Richard Skone verfasserin aut Immune and metabolic markers for identifying and investigating severe Coronavirus disease and Sepsis in children and young people (pSeP/COVID ChYP study): protocol for a prospective cohort study 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction Early recognition and appropriate management of paediatric sepsis are known to improve outcomes. A previous system’s biology investigation of the systemic immune response in neonates to sepsis identified immune and metabolic markers that showed high accuracy for detecting bacterial infection. Further gene expression markers have also been reported previously in the paediatric age group for discriminating sepsis from control cases. More recently, specific gene signatures were identified to discriminate between COVID-19 and its associated inflammatory sequelae. Through the current prospective cohort study, we aim to evaluate immune and metabolic blood markers which discriminate between sepses (including COVID-19) from other acute illnesses in critically unwell children and young persons, up to 18 years of age.Methods and analysis We describe a prospective cohort study for comparing the immune and metabolic whole-blood markers in patients with sepsis, COVID-19 and other illnesses. Clinical phenotyping and blood culture test results will provide a reference standard to evaluate the performance of blood markers from the research sample analysis. Serial sampling of whole blood (50 μL each) will be collected from children admitted to intensive care and with an acute illness to follow time dependent changes in biomarkers. An integrated lipidomics and RNASeq transcriptomics analyses will be conducted to evaluate immune-metabolic networks that discriminate sepsis and COVID-19 from other acute illnesses. This study received approval for deferred consent.Ethics and dissemination The study has received research ethics committee approval from the Yorkshire and Humber Leeds West Research Ethics Committee 2 (reference 20/YH/0214; IRAS reference 250612). Submission of study results for publication will involve making available all anonymised primary and processed data on public repository sites.Trial registration number NCT04904523. Medicine R Daniel White verfasserin aut Bethan Phillips verfasserin aut Jennifer Evans verfasserin aut Robert Andrews verfasserin aut Benjamin Saunders verfasserin aut Kerry Hood verfasserin aut Mallinath Chakraborty verfasserin aut Sarah Edkins verfasserin aut Barbara Paquete verfasserin aut Sian Foulkes verfasserin aut Anna Barrow verfasserin aut Siske Struik verfasserin aut Valerie O'Donnell verfasserin aut Sara Ali verfasserin aut Patrícia R S Rodrigues verfasserin aut Angela Strang verfasserin aut Summia Zaher verfasserin aut Simran Sharma verfasserin aut Luke C Davies verfasserin aut Linda Moet verfasserin aut James E McLaren verfasserin aut Gareth L Watson verfasserin aut Peter Ghazal verfasserin aut Edward Parkinson verfasserin aut Sivakumar Oruganti verfasserin aut William John Watkins verfasserin aut Selyf Shapey verfasserin aut Rim al Samsam verfasserin aut Malcolm Gajraj verfasserin aut Michelle Jardine verfasserin aut Jong Eun Song verfasserin aut Lloyd Abood verfasserin aut Sarah Joanne Kotecha verfasserin aut Awen Evans verfasserin aut Iona Buchanan verfasserin aut Susan Bowes verfasserin aut Begum Ali verfasserin aut Maya Gore verfasserin aut Rhian Thomas-Turner verfasserin aut Federico Liberatore verfasserin aut Thomas Woolley verfasserin aut In BMJ Open BMJ Publishing Group, 2011 13(2023), 3 (DE-627)654747075 (DE-600)2599832-8 20446055 nnns volume:13 year:2023 number:3 https://doi.org/10.1136/bmjopen-2022-067002 kostenfrei https://doaj.org/article/2d1c8f29b48f4539bf38b44ed3678f38 kostenfrei https://bmjopen.bmj.com/content/13/3/e067002.full kostenfrei https://doaj.org/toc/2044-6055 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_375 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 3 |
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10.1136/bmjopen-2022-067002 doi (DE-627)DOAJ087493934 (DE-599)DOAJ2d1c8f29b48f4539bf38b44ed3678f38 DE-627 ger DE-627 rakwb eng Richard Skone verfasserin aut Immune and metabolic markers for identifying and investigating severe Coronavirus disease and Sepsis in children and young people (pSeP/COVID ChYP study): protocol for a prospective cohort study 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction Early recognition and appropriate management of paediatric sepsis are known to improve outcomes. A previous system’s biology investigation of the systemic immune response in neonates to sepsis identified immune and metabolic markers that showed high accuracy for detecting bacterial infection. Further gene expression markers have also been reported previously in the paediatric age group for discriminating sepsis from control cases. More recently, specific gene signatures were identified to discriminate between COVID-19 and its associated inflammatory sequelae. Through the current prospective cohort study, we aim to evaluate immune and metabolic blood markers which discriminate between sepses (including COVID-19) from other acute illnesses in critically unwell children and young persons, up to 18 years of age.Methods and analysis We describe a prospective cohort study for comparing the immune and metabolic whole-blood markers in patients with sepsis, COVID-19 and other illnesses. Clinical phenotyping and blood culture test results will provide a reference standard to evaluate the performance of blood markers from the research sample analysis. Serial sampling of whole blood (50 μL each) will be collected from children admitted to intensive care and with an acute illness to follow time dependent changes in biomarkers. An integrated lipidomics and RNASeq transcriptomics analyses will be conducted to evaluate immune-metabolic networks that discriminate sepsis and COVID-19 from other acute illnesses. This study received approval for deferred consent.Ethics and dissemination The study has received research ethics committee approval from the Yorkshire and Humber Leeds West Research Ethics Committee 2 (reference 20/YH/0214; IRAS reference 250612). Submission of study results for publication will involve making available all anonymised primary and processed data on public repository sites.Trial registration number NCT04904523. Medicine R Daniel White verfasserin aut Bethan Phillips verfasserin aut Jennifer Evans verfasserin aut Robert Andrews verfasserin aut Benjamin Saunders verfasserin aut Kerry Hood verfasserin aut Mallinath Chakraborty verfasserin aut Sarah Edkins verfasserin aut Barbara Paquete verfasserin aut Sian Foulkes verfasserin aut Anna Barrow verfasserin aut Siske Struik verfasserin aut Valerie O'Donnell verfasserin aut Sara Ali verfasserin aut Patrícia R S Rodrigues verfasserin aut Angela Strang verfasserin aut Summia Zaher verfasserin aut Simran Sharma verfasserin aut Luke C Davies verfasserin aut Linda Moet verfasserin aut James E McLaren verfasserin aut Gareth L Watson verfasserin aut Peter Ghazal verfasserin aut Edward Parkinson verfasserin aut Sivakumar Oruganti verfasserin aut William John Watkins verfasserin aut Selyf Shapey verfasserin aut Rim al Samsam verfasserin aut Malcolm Gajraj verfasserin aut Michelle Jardine verfasserin aut Jong Eun Song verfasserin aut Lloyd Abood verfasserin aut Sarah Joanne Kotecha verfasserin aut Awen Evans verfasserin aut Iona Buchanan verfasserin aut Susan Bowes verfasserin aut Begum Ali verfasserin aut Maya Gore verfasserin aut Rhian Thomas-Turner verfasserin aut Federico Liberatore verfasserin aut Thomas Woolley verfasserin aut In BMJ Open BMJ Publishing Group, 2011 13(2023), 3 (DE-627)654747075 (DE-600)2599832-8 20446055 nnns volume:13 year:2023 number:3 https://doi.org/10.1136/bmjopen-2022-067002 kostenfrei https://doaj.org/article/2d1c8f29b48f4539bf38b44ed3678f38 kostenfrei https://bmjopen.bmj.com/content/13/3/e067002.full kostenfrei https://doaj.org/toc/2044-6055 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_375 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 3 |
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10.1136/bmjopen-2022-067002 doi (DE-627)DOAJ087493934 (DE-599)DOAJ2d1c8f29b48f4539bf38b44ed3678f38 DE-627 ger DE-627 rakwb eng Richard Skone verfasserin aut Immune and metabolic markers for identifying and investigating severe Coronavirus disease and Sepsis in children and young people (pSeP/COVID ChYP study): protocol for a prospective cohort study 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction Early recognition and appropriate management of paediatric sepsis are known to improve outcomes. A previous system’s biology investigation of the systemic immune response in neonates to sepsis identified immune and metabolic markers that showed high accuracy for detecting bacterial infection. Further gene expression markers have also been reported previously in the paediatric age group for discriminating sepsis from control cases. More recently, specific gene signatures were identified to discriminate between COVID-19 and its associated inflammatory sequelae. Through the current prospective cohort study, we aim to evaluate immune and metabolic blood markers which discriminate between sepses (including COVID-19) from other acute illnesses in critically unwell children and young persons, up to 18 years of age.Methods and analysis We describe a prospective cohort study for comparing the immune and metabolic whole-blood markers in patients with sepsis, COVID-19 and other illnesses. Clinical phenotyping and blood culture test results will provide a reference standard to evaluate the performance of blood markers from the research sample analysis. Serial sampling of whole blood (50 μL each) will be collected from children admitted to intensive care and with an acute illness to follow time dependent changes in biomarkers. An integrated lipidomics and RNASeq transcriptomics analyses will be conducted to evaluate immune-metabolic networks that discriminate sepsis and COVID-19 from other acute illnesses. This study received approval for deferred consent.Ethics and dissemination The study has received research ethics committee approval from the Yorkshire and Humber Leeds West Research Ethics Committee 2 (reference 20/YH/0214; IRAS reference 250612). Submission of study results for publication will involve making available all anonymised primary and processed data on public repository sites.Trial registration number NCT04904523. Medicine R Daniel White verfasserin aut Bethan Phillips verfasserin aut Jennifer Evans verfasserin aut Robert Andrews verfasserin aut Benjamin Saunders verfasserin aut Kerry Hood verfasserin aut Mallinath Chakraborty verfasserin aut Sarah Edkins verfasserin aut Barbara Paquete verfasserin aut Sian Foulkes verfasserin aut Anna Barrow verfasserin aut Siske Struik verfasserin aut Valerie O'Donnell verfasserin aut Sara Ali verfasserin aut Patrícia R S Rodrigues verfasserin aut Angela Strang verfasserin aut Summia Zaher verfasserin aut Simran Sharma verfasserin aut Luke C Davies verfasserin aut Linda Moet verfasserin aut James E McLaren verfasserin aut Gareth L Watson verfasserin aut Peter Ghazal verfasserin aut Edward Parkinson verfasserin aut Sivakumar Oruganti verfasserin aut William John Watkins verfasserin aut Selyf Shapey verfasserin aut Rim al Samsam verfasserin aut Malcolm Gajraj verfasserin aut Michelle Jardine verfasserin aut Jong Eun Song verfasserin aut Lloyd Abood verfasserin aut Sarah Joanne Kotecha verfasserin aut Awen Evans verfasserin aut Iona Buchanan verfasserin aut Susan Bowes verfasserin aut Begum Ali verfasserin aut Maya Gore verfasserin aut Rhian Thomas-Turner verfasserin aut Federico Liberatore verfasserin aut Thomas Woolley verfasserin aut In BMJ Open BMJ Publishing Group, 2011 13(2023), 3 (DE-627)654747075 (DE-600)2599832-8 20446055 nnns volume:13 year:2023 number:3 https://doi.org/10.1136/bmjopen-2022-067002 kostenfrei https://doaj.org/article/2d1c8f29b48f4539bf38b44ed3678f38 kostenfrei https://bmjopen.bmj.com/content/13/3/e067002.full kostenfrei https://doaj.org/toc/2044-6055 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_375 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 3 |
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10.1136/bmjopen-2022-067002 doi (DE-627)DOAJ087493934 (DE-599)DOAJ2d1c8f29b48f4539bf38b44ed3678f38 DE-627 ger DE-627 rakwb eng Richard Skone verfasserin aut Immune and metabolic markers for identifying and investigating severe Coronavirus disease and Sepsis in children and young people (pSeP/COVID ChYP study): protocol for a prospective cohort study 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction Early recognition and appropriate management of paediatric sepsis are known to improve outcomes. A previous system’s biology investigation of the systemic immune response in neonates to sepsis identified immune and metabolic markers that showed high accuracy for detecting bacterial infection. Further gene expression markers have also been reported previously in the paediatric age group for discriminating sepsis from control cases. More recently, specific gene signatures were identified to discriminate between COVID-19 and its associated inflammatory sequelae. Through the current prospective cohort study, we aim to evaluate immune and metabolic blood markers which discriminate between sepses (including COVID-19) from other acute illnesses in critically unwell children and young persons, up to 18 years of age.Methods and analysis We describe a prospective cohort study for comparing the immune and metabolic whole-blood markers in patients with sepsis, COVID-19 and other illnesses. Clinical phenotyping and blood culture test results will provide a reference standard to evaluate the performance of blood markers from the research sample analysis. Serial sampling of whole blood (50 μL each) will be collected from children admitted to intensive care and with an acute illness to follow time dependent changes in biomarkers. An integrated lipidomics and RNASeq transcriptomics analyses will be conducted to evaluate immune-metabolic networks that discriminate sepsis and COVID-19 from other acute illnesses. This study received approval for deferred consent.Ethics and dissemination The study has received research ethics committee approval from the Yorkshire and Humber Leeds West Research Ethics Committee 2 (reference 20/YH/0214; IRAS reference 250612). Submission of study results for publication will involve making available all anonymised primary and processed data on public repository sites.Trial registration number NCT04904523. Medicine R Daniel White verfasserin aut Bethan Phillips verfasserin aut Jennifer Evans verfasserin aut Robert Andrews verfasserin aut Benjamin Saunders verfasserin aut Kerry Hood verfasserin aut Mallinath Chakraborty verfasserin aut Sarah Edkins verfasserin aut Barbara Paquete verfasserin aut Sian Foulkes verfasserin aut Anna Barrow verfasserin aut Siske Struik verfasserin aut Valerie O'Donnell verfasserin aut Sara Ali verfasserin aut Patrícia R S Rodrigues verfasserin aut Angela Strang verfasserin aut Summia Zaher verfasserin aut Simran Sharma verfasserin aut Luke C Davies verfasserin aut Linda Moet verfasserin aut James E McLaren verfasserin aut Gareth L Watson verfasserin aut Peter Ghazal verfasserin aut Edward Parkinson verfasserin aut Sivakumar Oruganti verfasserin aut William John Watkins verfasserin aut Selyf Shapey verfasserin aut Rim al Samsam verfasserin aut Malcolm Gajraj verfasserin aut Michelle Jardine verfasserin aut Jong Eun Song verfasserin aut Lloyd Abood verfasserin aut Sarah Joanne Kotecha verfasserin aut Awen Evans verfasserin aut Iona Buchanan verfasserin aut Susan Bowes verfasserin aut Begum Ali verfasserin aut Maya Gore verfasserin aut Rhian Thomas-Turner verfasserin aut Federico Liberatore verfasserin aut Thomas Woolley verfasserin aut In BMJ Open BMJ Publishing Group, 2011 13(2023), 3 (DE-627)654747075 (DE-600)2599832-8 20446055 nnns volume:13 year:2023 number:3 https://doi.org/10.1136/bmjopen-2022-067002 kostenfrei https://doaj.org/article/2d1c8f29b48f4539bf38b44ed3678f38 kostenfrei https://bmjopen.bmj.com/content/13/3/e067002.full kostenfrei https://doaj.org/toc/2044-6055 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_375 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 3 |
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Richard Skone @@aut@@ Daniel White @@aut@@ Bethan Phillips @@aut@@ Jennifer Evans @@aut@@ Robert Andrews @@aut@@ Benjamin Saunders @@aut@@ Kerry Hood @@aut@@ Mallinath Chakraborty @@aut@@ Sarah Edkins @@aut@@ Barbara Paquete @@aut@@ Sian Foulkes @@aut@@ Anna Barrow @@aut@@ Siske Struik @@aut@@ Valerie O'Donnell @@aut@@ Sara Ali @@aut@@ Patrícia R S Rodrigues @@aut@@ Angela Strang @@aut@@ Summia Zaher @@aut@@ Simran Sharma @@aut@@ Luke C Davies @@aut@@ Linda Moet @@aut@@ James E McLaren @@aut@@ Gareth L Watson @@aut@@ Peter Ghazal @@aut@@ Edward Parkinson @@aut@@ Sivakumar Oruganti @@aut@@ William John Watkins @@aut@@ Selyf Shapey @@aut@@ Rim al Samsam @@aut@@ Malcolm Gajraj @@aut@@ Michelle Jardine @@aut@@ Jong Eun Song @@aut@@ Lloyd Abood @@aut@@ Sarah Joanne Kotecha @@aut@@ Awen Evans @@aut@@ Iona Buchanan @@aut@@ Susan Bowes @@aut@@ Begum Ali @@aut@@ Maya Gore @@aut@@ Rhian Thomas-Turner @@aut@@ Federico Liberatore @@aut@@ Thomas Woolley @@aut@@ |
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Immune and metabolic markers for identifying and investigating severe Coronavirus disease and Sepsis in children and young people (pSeP/COVID ChYP study): protocol for a prospective cohort study |
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Introduction Early recognition and appropriate management of paediatric sepsis are known to improve outcomes. A previous system’s biology investigation of the systemic immune response in neonates to sepsis identified immune and metabolic markers that showed high accuracy for detecting bacterial infection. Further gene expression markers have also been reported previously in the paediatric age group for discriminating sepsis from control cases. More recently, specific gene signatures were identified to discriminate between COVID-19 and its associated inflammatory sequelae. Through the current prospective cohort study, we aim to evaluate immune and metabolic blood markers which discriminate between sepses (including COVID-19) from other acute illnesses in critically unwell children and young persons, up to 18 years of age.Methods and analysis We describe a prospective cohort study for comparing the immune and metabolic whole-blood markers in patients with sepsis, COVID-19 and other illnesses. Clinical phenotyping and blood culture test results will provide a reference standard to evaluate the performance of blood markers from the research sample analysis. Serial sampling of whole blood (50 μL each) will be collected from children admitted to intensive care and with an acute illness to follow time dependent changes in biomarkers. An integrated lipidomics and RNASeq transcriptomics analyses will be conducted to evaluate immune-metabolic networks that discriminate sepsis and COVID-19 from other acute illnesses. This study received approval for deferred consent.Ethics and dissemination The study has received research ethics committee approval from the Yorkshire and Humber Leeds West Research Ethics Committee 2 (reference 20/YH/0214; IRAS reference 250612). Submission of study results for publication will involve making available all anonymised primary and processed data on public repository sites.Trial registration number NCT04904523. |
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Introduction Early recognition and appropriate management of paediatric sepsis are known to improve outcomes. A previous system’s biology investigation of the systemic immune response in neonates to sepsis identified immune and metabolic markers that showed high accuracy for detecting bacterial infection. Further gene expression markers have also been reported previously in the paediatric age group for discriminating sepsis from control cases. More recently, specific gene signatures were identified to discriminate between COVID-19 and its associated inflammatory sequelae. Through the current prospective cohort study, we aim to evaluate immune and metabolic blood markers which discriminate between sepses (including COVID-19) from other acute illnesses in critically unwell children and young persons, up to 18 years of age.Methods and analysis We describe a prospective cohort study for comparing the immune and metabolic whole-blood markers in patients with sepsis, COVID-19 and other illnesses. Clinical phenotyping and blood culture test results will provide a reference standard to evaluate the performance of blood markers from the research sample analysis. Serial sampling of whole blood (50 μL each) will be collected from children admitted to intensive care and with an acute illness to follow time dependent changes in biomarkers. An integrated lipidomics and RNASeq transcriptomics analyses will be conducted to evaluate immune-metabolic networks that discriminate sepsis and COVID-19 from other acute illnesses. This study received approval for deferred consent.Ethics and dissemination The study has received research ethics committee approval from the Yorkshire and Humber Leeds West Research Ethics Committee 2 (reference 20/YH/0214; IRAS reference 250612). Submission of study results for publication will involve making available all anonymised primary and processed data on public repository sites.Trial registration number NCT04904523. |
| abstract_unstemmed |
Introduction Early recognition and appropriate management of paediatric sepsis are known to improve outcomes. A previous system’s biology investigation of the systemic immune response in neonates to sepsis identified immune and metabolic markers that showed high accuracy for detecting bacterial infection. Further gene expression markers have also been reported previously in the paediatric age group for discriminating sepsis from control cases. More recently, specific gene signatures were identified to discriminate between COVID-19 and its associated inflammatory sequelae. Through the current prospective cohort study, we aim to evaluate immune and metabolic blood markers which discriminate between sepses (including COVID-19) from other acute illnesses in critically unwell children and young persons, up to 18 years of age.Methods and analysis We describe a prospective cohort study for comparing the immune and metabolic whole-blood markers in patients with sepsis, COVID-19 and other illnesses. Clinical phenotyping and blood culture test results will provide a reference standard to evaluate the performance of blood markers from the research sample analysis. Serial sampling of whole blood (50 μL each) will be collected from children admitted to intensive care and with an acute illness to follow time dependent changes in biomarkers. An integrated lipidomics and RNASeq transcriptomics analyses will be conducted to evaluate immune-metabolic networks that discriminate sepsis and COVID-19 from other acute illnesses. This study received approval for deferred consent.Ethics and dissemination The study has received research ethics committee approval from the Yorkshire and Humber Leeds West Research Ethics Committee 2 (reference 20/YH/0214; IRAS reference 250612). Submission of study results for publication will involve making available all anonymised primary and processed data on public repository sites.Trial registration number NCT04904523. |
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