Temperature-induced embryonic diapause in chickens is mediated by PKC-NF-κB-IRF1 signaling

Abstract Background Embryonic diapause (dormancy) is a state of temporary arrest of embryonic development that is triggered by unfavorable conditions and serves as an evolutionary strategy to ensure reproductive survival. Unlike maternally-controlled embryonic diapause in mammals, chicken embryonic...
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Autor*in:	Junxiao Ren [verfasserIn] Zhengzheng Hu [verfasserIn] Quanlin Li [verfasserIn] Shuang Gu [verfasserIn] Fangren Lan [verfasserIn] Xiqiong Wang [verfasserIn] Jianbo Li [verfasserIn] Junying Li [verfasserIn] Liwa Shao [verfasserIn] Ning Yang [verfasserIn] Congjiao Sun [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	Diapause Embryo Cell proliferation Cold stress PKC-NF-κB signaling

Übergeordnetes Werk:	In: BMC Biology - BMC, 2003, 21(2023), 1, Seite 20
Übergeordnetes Werk:	volume:21 ; year:2023 ; number:1 ; pages:20

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/s12915-023-01550-0

Katalog-ID:	DOAJ087701804

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520			\|a Abstract Background Embryonic diapause (dormancy) is a state of temporary arrest of embryonic development that is triggered by unfavorable conditions and serves as an evolutionary strategy to ensure reproductive survival. Unlike maternally-controlled embryonic diapause in mammals, chicken embryonic diapause is critically dependent on the environmental temperature. However, the molecular control of diapause in avian species remains largely uncharacterized. In this study, we evaluated the dynamic transcriptomic and phosphoproteomic profiles of chicken embryos in pre-diapause, diapause, and reactivated states. Results Our data demonstrated a characteristic gene expression pattern in effects on cell survival-associated and stress response signaling pathways. Unlike mammalian diapause, mTOR signaling is not responsible for chicken diapause. However, cold stress responsive genes, such as IRF1, were identified as key regulators of diapause. Further in vitro investigation showed that cold stress-induced transcription of IRF1 was dependent on the PKC-NF-κB signaling pathway, providing a mechanism for proliferation arrest during diapause. Consistently, in vivo overexpression of IRF1 in diapause embryos blocked reactivation after restoration of developmental temperatures. Conclusions We concluded that embryonic diapause in chicken is characterized by proliferation arrest, which is the same with other spices. However, chicken embryonic diapause is strictly correlated with the cold stress signal and mediated by PKC-NF-κB-IRF1 signaling, which distinguish chicken diapause from the mTOR based diapause in mammals.
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allfields	10.1186/s12915-023-01550-0 doi (DE-627)DOAJ087701804 (DE-599)DOAJd67154216653473e9c1e18c8ae594ccf DE-627 ger DE-627 rakwb eng QH301-705.5 Junxiao Ren verfasserin aut Temperature-induced embryonic diapause in chickens is mediated by PKC-NF-κB-IRF1 signaling 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Embryonic diapause (dormancy) is a state of temporary arrest of embryonic development that is triggered by unfavorable conditions and serves as an evolutionary strategy to ensure reproductive survival. Unlike maternally-controlled embryonic diapause in mammals, chicken embryonic diapause is critically dependent on the environmental temperature. However, the molecular control of diapause in avian species remains largely uncharacterized. In this study, we evaluated the dynamic transcriptomic and phosphoproteomic profiles of chicken embryos in pre-diapause, diapause, and reactivated states. Results Our data demonstrated a characteristic gene expression pattern in effects on cell survival-associated and stress response signaling pathways. Unlike mammalian diapause, mTOR signaling is not responsible for chicken diapause. However, cold stress responsive genes, such as IRF1, were identified as key regulators of diapause. Further in vitro investigation showed that cold stress-induced transcription of IRF1 was dependent on the PKC-NF-κB signaling pathway, providing a mechanism for proliferation arrest during diapause. Consistently, in vivo overexpression of IRF1 in diapause embryos blocked reactivation after restoration of developmental temperatures. Conclusions We concluded that embryonic diapause in chicken is characterized by proliferation arrest, which is the same with other spices. However, chicken embryonic diapause is strictly correlated with the cold stress signal and mediated by PKC-NF-κB-IRF1 signaling, which distinguish chicken diapause from the mTOR based diapause in mammals. Diapause Embryo Cell proliferation Cold stress PKC-NF-κB signaling Biology (General) Zhengzheng Hu verfasserin aut Quanlin Li verfasserin aut Shuang Gu verfasserin aut Fangren Lan verfasserin aut Xiqiong Wang verfasserin aut Jianbo Li verfasserin aut Junying Li verfasserin aut Liwa Shao verfasserin aut Ning Yang verfasserin aut Congjiao Sun verfasserin aut In BMC Biology BMC, 2003 21(2023), 1, Seite 20 (DE-627)377757241 (DE-600)2133020-7 17417007 nnns volume:21 year:2023 number:1 pages:20 https://doi.org/10.1186/s12915-023-01550-0 kostenfrei https://doaj.org/article/d67154216653473e9c1e18c8ae594ccf kostenfrei https://doi.org/10.1186/s12915-023-01550-0 kostenfrei https://doaj.org/toc/1741-7007 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2023 1 20
spelling	10.1186/s12915-023-01550-0 doi (DE-627)DOAJ087701804 (DE-599)DOAJd67154216653473e9c1e18c8ae594ccf DE-627 ger DE-627 rakwb eng QH301-705.5 Junxiao Ren verfasserin aut Temperature-induced embryonic diapause in chickens is mediated by PKC-NF-κB-IRF1 signaling 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Embryonic diapause (dormancy) is a state of temporary arrest of embryonic development that is triggered by unfavorable conditions and serves as an evolutionary strategy to ensure reproductive survival. Unlike maternally-controlled embryonic diapause in mammals, chicken embryonic diapause is critically dependent on the environmental temperature. However, the molecular control of diapause in avian species remains largely uncharacterized. In this study, we evaluated the dynamic transcriptomic and phosphoproteomic profiles of chicken embryos in pre-diapause, diapause, and reactivated states. Results Our data demonstrated a characteristic gene expression pattern in effects on cell survival-associated and stress response signaling pathways. Unlike mammalian diapause, mTOR signaling is not responsible for chicken diapause. However, cold stress responsive genes, such as IRF1, were identified as key regulators of diapause. Further in vitro investigation showed that cold stress-induced transcription of IRF1 was dependent on the PKC-NF-κB signaling pathway, providing a mechanism for proliferation arrest during diapause. Consistently, in vivo overexpression of IRF1 in diapause embryos blocked reactivation after restoration of developmental temperatures. Conclusions We concluded that embryonic diapause in chicken is characterized by proliferation arrest, which is the same with other spices. However, chicken embryonic diapause is strictly correlated with the cold stress signal and mediated by PKC-NF-κB-IRF1 signaling, which distinguish chicken diapause from the mTOR based diapause in mammals. Diapause Embryo Cell proliferation Cold stress PKC-NF-κB signaling Biology (General) Zhengzheng Hu verfasserin aut Quanlin Li verfasserin aut Shuang Gu verfasserin aut Fangren Lan verfasserin aut Xiqiong Wang verfasserin aut Jianbo Li verfasserin aut Junying Li verfasserin aut Liwa Shao verfasserin aut Ning Yang verfasserin aut Congjiao Sun verfasserin aut In BMC Biology BMC, 2003 21(2023), 1, Seite 20 (DE-627)377757241 (DE-600)2133020-7 17417007 nnns volume:21 year:2023 number:1 pages:20 https://doi.org/10.1186/s12915-023-01550-0 kostenfrei https://doaj.org/article/d67154216653473e9c1e18c8ae594ccf kostenfrei https://doi.org/10.1186/s12915-023-01550-0 kostenfrei https://doaj.org/toc/1741-7007 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2023 1 20
allfields_unstemmed	10.1186/s12915-023-01550-0 doi (DE-627)DOAJ087701804 (DE-599)DOAJd67154216653473e9c1e18c8ae594ccf DE-627 ger DE-627 rakwb eng QH301-705.5 Junxiao Ren verfasserin aut Temperature-induced embryonic diapause in chickens is mediated by PKC-NF-κB-IRF1 signaling 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Embryonic diapause (dormancy) is a state of temporary arrest of embryonic development that is triggered by unfavorable conditions and serves as an evolutionary strategy to ensure reproductive survival. Unlike maternally-controlled embryonic diapause in mammals, chicken embryonic diapause is critically dependent on the environmental temperature. However, the molecular control of diapause in avian species remains largely uncharacterized. In this study, we evaluated the dynamic transcriptomic and phosphoproteomic profiles of chicken embryos in pre-diapause, diapause, and reactivated states. Results Our data demonstrated a characteristic gene expression pattern in effects on cell survival-associated and stress response signaling pathways. Unlike mammalian diapause, mTOR signaling is not responsible for chicken diapause. However, cold stress responsive genes, such as IRF1, were identified as key regulators of diapause. Further in vitro investigation showed that cold stress-induced transcription of IRF1 was dependent on the PKC-NF-κB signaling pathway, providing a mechanism for proliferation arrest during diapause. Consistently, in vivo overexpression of IRF1 in diapause embryos blocked reactivation after restoration of developmental temperatures. Conclusions We concluded that embryonic diapause in chicken is characterized by proliferation arrest, which is the same with other spices. However, chicken embryonic diapause is strictly correlated with the cold stress signal and mediated by PKC-NF-κB-IRF1 signaling, which distinguish chicken diapause from the mTOR based diapause in mammals. Diapause Embryo Cell proliferation Cold stress PKC-NF-κB signaling Biology (General) Zhengzheng Hu verfasserin aut Quanlin Li verfasserin aut Shuang Gu verfasserin aut Fangren Lan verfasserin aut Xiqiong Wang verfasserin aut Jianbo Li verfasserin aut Junying Li verfasserin aut Liwa Shao verfasserin aut Ning Yang verfasserin aut Congjiao Sun verfasserin aut In BMC Biology BMC, 2003 21(2023), 1, Seite 20 (DE-627)377757241 (DE-600)2133020-7 17417007 nnns volume:21 year:2023 number:1 pages:20 https://doi.org/10.1186/s12915-023-01550-0 kostenfrei https://doaj.org/article/d67154216653473e9c1e18c8ae594ccf kostenfrei https://doi.org/10.1186/s12915-023-01550-0 kostenfrei https://doaj.org/toc/1741-7007 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2023 1 20
allfieldsGer	10.1186/s12915-023-01550-0 doi (DE-627)DOAJ087701804 (DE-599)DOAJd67154216653473e9c1e18c8ae594ccf DE-627 ger DE-627 rakwb eng QH301-705.5 Junxiao Ren verfasserin aut Temperature-induced embryonic diapause in chickens is mediated by PKC-NF-κB-IRF1 signaling 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Embryonic diapause (dormancy) is a state of temporary arrest of embryonic development that is triggered by unfavorable conditions and serves as an evolutionary strategy to ensure reproductive survival. Unlike maternally-controlled embryonic diapause in mammals, chicken embryonic diapause is critically dependent on the environmental temperature. However, the molecular control of diapause in avian species remains largely uncharacterized. In this study, we evaluated the dynamic transcriptomic and phosphoproteomic profiles of chicken embryos in pre-diapause, diapause, and reactivated states. Results Our data demonstrated a characteristic gene expression pattern in effects on cell survival-associated and stress response signaling pathways. Unlike mammalian diapause, mTOR signaling is not responsible for chicken diapause. However, cold stress responsive genes, such as IRF1, were identified as key regulators of diapause. Further in vitro investigation showed that cold stress-induced transcription of IRF1 was dependent on the PKC-NF-κB signaling pathway, providing a mechanism for proliferation arrest during diapause. Consistently, in vivo overexpression of IRF1 in diapause embryos blocked reactivation after restoration of developmental temperatures. Conclusions We concluded that embryonic diapause in chicken is characterized by proliferation arrest, which is the same with other spices. However, chicken embryonic diapause is strictly correlated with the cold stress signal and mediated by PKC-NF-κB-IRF1 signaling, which distinguish chicken diapause from the mTOR based diapause in mammals. Diapause Embryo Cell proliferation Cold stress PKC-NF-κB signaling Biology (General) Zhengzheng Hu verfasserin aut Quanlin Li verfasserin aut Shuang Gu verfasserin aut Fangren Lan verfasserin aut Xiqiong Wang verfasserin aut Jianbo Li verfasserin aut Junying Li verfasserin aut Liwa Shao verfasserin aut Ning Yang verfasserin aut Congjiao Sun verfasserin aut In BMC Biology BMC, 2003 21(2023), 1, Seite 20 (DE-627)377757241 (DE-600)2133020-7 17417007 nnns volume:21 year:2023 number:1 pages:20 https://doi.org/10.1186/s12915-023-01550-0 kostenfrei https://doaj.org/article/d67154216653473e9c1e18c8ae594ccf kostenfrei https://doi.org/10.1186/s12915-023-01550-0 kostenfrei https://doaj.org/toc/1741-7007 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2023 1 20
allfieldsSound	10.1186/s12915-023-01550-0 doi (DE-627)DOAJ087701804 (DE-599)DOAJd67154216653473e9c1e18c8ae594ccf DE-627 ger DE-627 rakwb eng QH301-705.5 Junxiao Ren verfasserin aut Temperature-induced embryonic diapause in chickens is mediated by PKC-NF-κB-IRF1 signaling 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Embryonic diapause (dormancy) is a state of temporary arrest of embryonic development that is triggered by unfavorable conditions and serves as an evolutionary strategy to ensure reproductive survival. Unlike maternally-controlled embryonic diapause in mammals, chicken embryonic diapause is critically dependent on the environmental temperature. However, the molecular control of diapause in avian species remains largely uncharacterized. In this study, we evaluated the dynamic transcriptomic and phosphoproteomic profiles of chicken embryos in pre-diapause, diapause, and reactivated states. Results Our data demonstrated a characteristic gene expression pattern in effects on cell survival-associated and stress response signaling pathways. Unlike mammalian diapause, mTOR signaling is not responsible for chicken diapause. However, cold stress responsive genes, such as IRF1, were identified as key regulators of diapause. Further in vitro investigation showed that cold stress-induced transcription of IRF1 was dependent on the PKC-NF-κB signaling pathway, providing a mechanism for proliferation arrest during diapause. Consistently, in vivo overexpression of IRF1 in diapause embryos blocked reactivation after restoration of developmental temperatures. Conclusions We concluded that embryonic diapause in chicken is characterized by proliferation arrest, which is the same with other spices. However, chicken embryonic diapause is strictly correlated with the cold stress signal and mediated by PKC-NF-κB-IRF1 signaling, which distinguish chicken diapause from the mTOR based diapause in mammals. Diapause Embryo Cell proliferation Cold stress PKC-NF-κB signaling Biology (General) Zhengzheng Hu verfasserin aut Quanlin Li verfasserin aut Shuang Gu verfasserin aut Fangren Lan verfasserin aut Xiqiong Wang verfasserin aut Jianbo Li verfasserin aut Junying Li verfasserin aut Liwa Shao verfasserin aut Ning Yang verfasserin aut Congjiao Sun verfasserin aut In BMC Biology BMC, 2003 21(2023), 1, Seite 20 (DE-627)377757241 (DE-600)2133020-7 17417007 nnns volume:21 year:2023 number:1 pages:20 https://doi.org/10.1186/s12915-023-01550-0 kostenfrei https://doaj.org/article/d67154216653473e9c1e18c8ae594ccf kostenfrei https://doi.org/10.1186/s12915-023-01550-0 kostenfrei https://doaj.org/toc/1741-7007 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2023 1 20
language	English
source	In BMC Biology 21(2023), 1, Seite 20 volume:21 year:2023 number:1 pages:20
sourceStr	In BMC Biology 21(2023), 1, Seite 20 volume:21 year:2023 number:1 pages:20
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Diapause Embryo Cell proliferation Cold stress PKC-NF-κB signaling Biology (General)
isfreeaccess_bool	true
container_title	BMC Biology
authorswithroles_txt_mv	Junxiao Ren @@aut@@ Zhengzheng Hu @@aut@@ Quanlin Li @@aut@@ Shuang Gu @@aut@@ Fangren Lan @@aut@@ Xiqiong Wang @@aut@@ Jianbo Li @@aut@@ Junying Li @@aut@@ Liwa Shao @@aut@@ Ning Yang @@aut@@ Congjiao Sun @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	377757241
id	DOAJ087701804
language_de	englisch
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Unlike maternally-controlled embryonic diapause in mammals, chicken embryonic diapause is critically dependent on the environmental temperature. However, the molecular control of diapause in avian species remains largely uncharacterized. In this study, we evaluated the dynamic transcriptomic and phosphoproteomic profiles of chicken embryos in pre-diapause, diapause, and reactivated states. Results Our data demonstrated a characteristic gene expression pattern in effects on cell survival-associated and stress response signaling pathways. Unlike mammalian diapause, mTOR signaling is not responsible for chicken diapause. However, cold stress responsive genes, such as IRF1, were identified as key regulators of diapause. Further in vitro investigation showed that cold stress-induced transcription of IRF1 was dependent on the PKC-NF-κB signaling pathway, providing a mechanism for proliferation arrest during diapause. 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callnumber-first	Q - Science
author	Junxiao Ren
spellingShingle	Junxiao Ren misc QH301-705.5 misc Diapause misc Embryo misc Cell proliferation misc Cold stress misc PKC-NF-κB signaling misc Biology (General) Temperature-induced embryonic diapause in chickens is mediated by PKC-NF-κB-IRF1 signaling
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topic_title	QH301-705.5 Temperature-induced embryonic diapause in chickens is mediated by PKC-NF-κB-IRF1 signaling Diapause Embryo Cell proliferation Cold stress PKC-NF-κB signaling
topic	misc QH301-705.5 misc Diapause misc Embryo misc Cell proliferation misc Cold stress misc PKC-NF-κB signaling misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc Diapause misc Embryo misc Cell proliferation misc Cold stress misc PKC-NF-κB signaling misc Biology (General)
topic_browse	misc QH301-705.5 misc Diapause misc Embryo misc Cell proliferation misc Cold stress misc PKC-NF-κB signaling misc Biology (General)
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title	Temperature-induced embryonic diapause in chickens is mediated by PKC-NF-κB-IRF1 signaling
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title_full	Temperature-induced embryonic diapause in chickens is mediated by PKC-NF-κB-IRF1 signaling
author_sort	Junxiao Ren
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author_browse	Junxiao Ren Zhengzheng Hu Quanlin Li Shuang Gu Fangren Lan Xiqiong Wang Jianbo Li Junying Li Liwa Shao Ning Yang Congjiao Sun
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title_sort	temperature-induced embryonic diapause in chickens is mediated by pkc-nf-κb-irf1 signaling
callnumber	QH301-705.5
title_auth	Temperature-induced embryonic diapause in chickens is mediated by PKC-NF-κB-IRF1 signaling
abstract	Abstract Background Embryonic diapause (dormancy) is a state of temporary arrest of embryonic development that is triggered by unfavorable conditions and serves as an evolutionary strategy to ensure reproductive survival. Unlike maternally-controlled embryonic diapause in mammals, chicken embryonic diapause is critically dependent on the environmental temperature. However, the molecular control of diapause in avian species remains largely uncharacterized. In this study, we evaluated the dynamic transcriptomic and phosphoproteomic profiles of chicken embryos in pre-diapause, diapause, and reactivated states. Results Our data demonstrated a characteristic gene expression pattern in effects on cell survival-associated and stress response signaling pathways. Unlike mammalian diapause, mTOR signaling is not responsible for chicken diapause. However, cold stress responsive genes, such as IRF1, were identified as key regulators of diapause. Further in vitro investigation showed that cold stress-induced transcription of IRF1 was dependent on the PKC-NF-κB signaling pathway, providing a mechanism for proliferation arrest during diapause. Consistently, in vivo overexpression of IRF1 in diapause embryos blocked reactivation after restoration of developmental temperatures. Conclusions We concluded that embryonic diapause in chicken is characterized by proliferation arrest, which is the same with other spices. However, chicken embryonic diapause is strictly correlated with the cold stress signal and mediated by PKC-NF-κB-IRF1 signaling, which distinguish chicken diapause from the mTOR based diapause in mammals.
abstractGer	Abstract Background Embryonic diapause (dormancy) is a state of temporary arrest of embryonic development that is triggered by unfavorable conditions and serves as an evolutionary strategy to ensure reproductive survival. Unlike maternally-controlled embryonic diapause in mammals, chicken embryonic diapause is critically dependent on the environmental temperature. However, the molecular control of diapause in avian species remains largely uncharacterized. In this study, we evaluated the dynamic transcriptomic and phosphoproteomic profiles of chicken embryos in pre-diapause, diapause, and reactivated states. Results Our data demonstrated a characteristic gene expression pattern in effects on cell survival-associated and stress response signaling pathways. Unlike mammalian diapause, mTOR signaling is not responsible for chicken diapause. However, cold stress responsive genes, such as IRF1, were identified as key regulators of diapause. Further in vitro investigation showed that cold stress-induced transcription of IRF1 was dependent on the PKC-NF-κB signaling pathway, providing a mechanism for proliferation arrest during diapause. Consistently, in vivo overexpression of IRF1 in diapause embryos blocked reactivation after restoration of developmental temperatures. Conclusions We concluded that embryonic diapause in chicken is characterized by proliferation arrest, which is the same with other spices. However, chicken embryonic diapause is strictly correlated with the cold stress signal and mediated by PKC-NF-κB-IRF1 signaling, which distinguish chicken diapause from the mTOR based diapause in mammals.
abstract_unstemmed	Abstract Background Embryonic diapause (dormancy) is a state of temporary arrest of embryonic development that is triggered by unfavorable conditions and serves as an evolutionary strategy to ensure reproductive survival. Unlike maternally-controlled embryonic diapause in mammals, chicken embryonic diapause is critically dependent on the environmental temperature. However, the molecular control of diapause in avian species remains largely uncharacterized. In this study, we evaluated the dynamic transcriptomic and phosphoproteomic profiles of chicken embryos in pre-diapause, diapause, and reactivated states. Results Our data demonstrated a characteristic gene expression pattern in effects on cell survival-associated and stress response signaling pathways. Unlike mammalian diapause, mTOR signaling is not responsible for chicken diapause. However, cold stress responsive genes, such as IRF1, were identified as key regulators of diapause. Further in vitro investigation showed that cold stress-induced transcription of IRF1 was dependent on the PKC-NF-κB signaling pathway, providing a mechanism for proliferation arrest during diapause. Consistently, in vivo overexpression of IRF1 in diapause embryos blocked reactivation after restoration of developmental temperatures. Conclusions We concluded that embryonic diapause in chicken is characterized by proliferation arrest, which is the same with other spices. However, chicken embryonic diapause is strictly correlated with the cold stress signal and mediated by PKC-NF-κB-IRF1 signaling, which distinguish chicken diapause from the mTOR based diapause in mammals.
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title_short	Temperature-induced embryonic diapause in chickens is mediated by PKC-NF-κB-IRF1 signaling
url	https://doi.org/10.1186/s12915-023-01550-0 https://doaj.org/article/d67154216653473e9c1e18c8ae594ccf https://doaj.org/toc/1741-7007
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Temperature-induced embryonic diapause in chickens is mediated by PKC-NF-κB-IRF1 signaling

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