Design and Validation of a Custom-Made Hyperspectral Microscope Imaging System for Biomedical Applications

Hyperspectral microscope imaging (HMI) is an emerging modality that integrates spatial information collected by standard laboratory microscopy and the spectral-based contrast obtained by hyperspectral imaging and may be instrumental in establishing novel quantitative diagnostic methodologies, partic...
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Autor*in:	Jošt Stergar [verfasserIn] Rok Hren [verfasserIn] Matija Milanič [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	biomedical optics microscopy hyperspectral imaging spectroscopy instrumentation

Übergeordnetes Werk:	In: Sensors - MDPI AG, 2003, 23(2023), 5, p 2374
Übergeordnetes Werk:	volume:23 ; year:2023 ; number:5, p 2374

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/s23052374

Katalog-ID:	DOAJ087973219

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allfields	10.3390/s23052374 doi (DE-627)DOAJ087973219 (DE-599)DOAJ45ff3451fedb4489aaa3d5b1560c01ab DE-627 ger DE-627 rakwb eng TP1-1185 Jošt Stergar verfasserin aut Design and Validation of a Custom-Made Hyperspectral Microscope Imaging System for Biomedical Applications 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hyperspectral microscope imaging (HMI) is an emerging modality that integrates spatial information collected by standard laboratory microscopy and the spectral-based contrast obtained by hyperspectral imaging and may be instrumental in establishing novel quantitative diagnostic methodologies, particularly in histopathology. Further expansion of HMI capabilities hinges upon the modularity and versatility of systems and their proper standardization. In this report, we describe the design, calibration, characterization, and validation of the custom-made laboratory HMI system based on a Zeiss Axiotron fully motorized microscope and a custom-developed Czerny-Turner-type monochromator. For these important steps, we rely on a previously designed calibration protocol. Validation of the system demonstrates a performance comparable to classic spectrometry laboratory systems. We further demonstrate validation against a laboratory hyperspectral imaging system for macroscopic samples, enabling future comparison of spectral imaging results across length scales. An example of the utility of our custom-made HMI system on a standard hematoxylin and eosin-stained histology slide is also shown. biomedical optics microscopy hyperspectral imaging spectroscopy instrumentation Chemical technology Rok Hren verfasserin aut Matija Milanič verfasserin aut In Sensors MDPI AG, 2003 23(2023), 5, p 2374 (DE-627)331640910 (DE-600)2052857-7 14248220 nnns volume:23 year:2023 number:5, p 2374 https://doi.org/10.3390/s23052374 kostenfrei https://doaj.org/article/45ff3451fedb4489aaa3d5b1560c01ab kostenfrei https://www.mdpi.com/1424-8220/23/5/2374 kostenfrei https://doaj.org/toc/1424-8220 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2111 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2023 5, p 2374
spelling	10.3390/s23052374 doi (DE-627)DOAJ087973219 (DE-599)DOAJ45ff3451fedb4489aaa3d5b1560c01ab DE-627 ger DE-627 rakwb eng TP1-1185 Jošt Stergar verfasserin aut Design and Validation of a Custom-Made Hyperspectral Microscope Imaging System for Biomedical Applications 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hyperspectral microscope imaging (HMI) is an emerging modality that integrates spatial information collected by standard laboratory microscopy and the spectral-based contrast obtained by hyperspectral imaging and may be instrumental in establishing novel quantitative diagnostic methodologies, particularly in histopathology. Further expansion of HMI capabilities hinges upon the modularity and versatility of systems and their proper standardization. In this report, we describe the design, calibration, characterization, and validation of the custom-made laboratory HMI system based on a Zeiss Axiotron fully motorized microscope and a custom-developed Czerny-Turner-type monochromator. For these important steps, we rely on a previously designed calibration protocol. Validation of the system demonstrates a performance comparable to classic spectrometry laboratory systems. We further demonstrate validation against a laboratory hyperspectral imaging system for macroscopic samples, enabling future comparison of spectral imaging results across length scales. An example of the utility of our custom-made HMI system on a standard hematoxylin and eosin-stained histology slide is also shown. biomedical optics microscopy hyperspectral imaging spectroscopy instrumentation Chemical technology Rok Hren verfasserin aut Matija Milanič verfasserin aut In Sensors MDPI AG, 2003 23(2023), 5, p 2374 (DE-627)331640910 (DE-600)2052857-7 14248220 nnns volume:23 year:2023 number:5, p 2374 https://doi.org/10.3390/s23052374 kostenfrei https://doaj.org/article/45ff3451fedb4489aaa3d5b1560c01ab kostenfrei https://www.mdpi.com/1424-8220/23/5/2374 kostenfrei https://doaj.org/toc/1424-8220 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2111 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2023 5, p 2374
allfields_unstemmed	10.3390/s23052374 doi (DE-627)DOAJ087973219 (DE-599)DOAJ45ff3451fedb4489aaa3d5b1560c01ab DE-627 ger DE-627 rakwb eng TP1-1185 Jošt Stergar verfasserin aut Design and Validation of a Custom-Made Hyperspectral Microscope Imaging System for Biomedical Applications 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hyperspectral microscope imaging (HMI) is an emerging modality that integrates spatial information collected by standard laboratory microscopy and the spectral-based contrast obtained by hyperspectral imaging and may be instrumental in establishing novel quantitative diagnostic methodologies, particularly in histopathology. Further expansion of HMI capabilities hinges upon the modularity and versatility of systems and their proper standardization. In this report, we describe the design, calibration, characterization, and validation of the custom-made laboratory HMI system based on a Zeiss Axiotron fully motorized microscope and a custom-developed Czerny-Turner-type monochromator. For these important steps, we rely on a previously designed calibration protocol. Validation of the system demonstrates a performance comparable to classic spectrometry laboratory systems. We further demonstrate validation against a laboratory hyperspectral imaging system for macroscopic samples, enabling future comparison of spectral imaging results across length scales. An example of the utility of our custom-made HMI system on a standard hematoxylin and eosin-stained histology slide is also shown. biomedical optics microscopy hyperspectral imaging spectroscopy instrumentation Chemical technology Rok Hren verfasserin aut Matija Milanič verfasserin aut In Sensors MDPI AG, 2003 23(2023), 5, p 2374 (DE-627)331640910 (DE-600)2052857-7 14248220 nnns volume:23 year:2023 number:5, p 2374 https://doi.org/10.3390/s23052374 kostenfrei https://doaj.org/article/45ff3451fedb4489aaa3d5b1560c01ab kostenfrei https://www.mdpi.com/1424-8220/23/5/2374 kostenfrei https://doaj.org/toc/1424-8220 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2111 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2023 5, p 2374
allfieldsGer	10.3390/s23052374 doi (DE-627)DOAJ087973219 (DE-599)DOAJ45ff3451fedb4489aaa3d5b1560c01ab DE-627 ger DE-627 rakwb eng TP1-1185 Jošt Stergar verfasserin aut Design and Validation of a Custom-Made Hyperspectral Microscope Imaging System for Biomedical Applications 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hyperspectral microscope imaging (HMI) is an emerging modality that integrates spatial information collected by standard laboratory microscopy and the spectral-based contrast obtained by hyperspectral imaging and may be instrumental in establishing novel quantitative diagnostic methodologies, particularly in histopathology. Further expansion of HMI capabilities hinges upon the modularity and versatility of systems and their proper standardization. In this report, we describe the design, calibration, characterization, and validation of the custom-made laboratory HMI system based on a Zeiss Axiotron fully motorized microscope and a custom-developed Czerny-Turner-type monochromator. For these important steps, we rely on a previously designed calibration protocol. Validation of the system demonstrates a performance comparable to classic spectrometry laboratory systems. We further demonstrate validation against a laboratory hyperspectral imaging system for macroscopic samples, enabling future comparison of spectral imaging results across length scales. An example of the utility of our custom-made HMI system on a standard hematoxylin and eosin-stained histology slide is also shown. biomedical optics microscopy hyperspectral imaging spectroscopy instrumentation Chemical technology Rok Hren verfasserin aut Matija Milanič verfasserin aut In Sensors MDPI AG, 2003 23(2023), 5, p 2374 (DE-627)331640910 (DE-600)2052857-7 14248220 nnns volume:23 year:2023 number:5, p 2374 https://doi.org/10.3390/s23052374 kostenfrei https://doaj.org/article/45ff3451fedb4489aaa3d5b1560c01ab kostenfrei https://www.mdpi.com/1424-8220/23/5/2374 kostenfrei https://doaj.org/toc/1424-8220 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2111 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2023 5, p 2374
allfieldsSound	10.3390/s23052374 doi (DE-627)DOAJ087973219 (DE-599)DOAJ45ff3451fedb4489aaa3d5b1560c01ab DE-627 ger DE-627 rakwb eng TP1-1185 Jošt Stergar verfasserin aut Design and Validation of a Custom-Made Hyperspectral Microscope Imaging System for Biomedical Applications 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hyperspectral microscope imaging (HMI) is an emerging modality that integrates spatial information collected by standard laboratory microscopy and the spectral-based contrast obtained by hyperspectral imaging and may be instrumental in establishing novel quantitative diagnostic methodologies, particularly in histopathology. Further expansion of HMI capabilities hinges upon the modularity and versatility of systems and their proper standardization. In this report, we describe the design, calibration, characterization, and validation of the custom-made laboratory HMI system based on a Zeiss Axiotron fully motorized microscope and a custom-developed Czerny-Turner-type monochromator. For these important steps, we rely on a previously designed calibration protocol. Validation of the system demonstrates a performance comparable to classic spectrometry laboratory systems. We further demonstrate validation against a laboratory hyperspectral imaging system for macroscopic samples, enabling future comparison of spectral imaging results across length scales. An example of the utility of our custom-made HMI system on a standard hematoxylin and eosin-stained histology slide is also shown. biomedical optics microscopy hyperspectral imaging spectroscopy instrumentation Chemical technology Rok Hren verfasserin aut Matija Milanič verfasserin aut In Sensors MDPI AG, 2003 23(2023), 5, p 2374 (DE-627)331640910 (DE-600)2052857-7 14248220 nnns volume:23 year:2023 number:5, p 2374 https://doi.org/10.3390/s23052374 kostenfrei https://doaj.org/article/45ff3451fedb4489aaa3d5b1560c01ab kostenfrei https://www.mdpi.com/1424-8220/23/5/2374 kostenfrei https://doaj.org/toc/1424-8220 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2111 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2023 5, p 2374
language	English
source	In Sensors 23(2023), 5, p 2374 volume:23 year:2023 number:5, p 2374
sourceStr	In Sensors 23(2023), 5, p 2374 volume:23 year:2023 number:5, p 2374
format_phy_str_mv	Article
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topic_facet	biomedical optics microscopy hyperspectral imaging spectroscopy instrumentation Chemical technology
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authorswithroles_txt_mv	Jošt Stergar @@aut@@ Rok Hren @@aut@@ Matija Milanič @@aut@@
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callnumber-first	T - Technology
author	Jošt Stergar
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topic_title	TP1-1185 Design and Validation of a Custom-Made Hyperspectral Microscope Imaging System for Biomedical Applications biomedical optics microscopy hyperspectral imaging spectroscopy instrumentation
topic	misc TP1-1185 misc biomedical optics misc microscopy misc hyperspectral imaging misc spectroscopy misc instrumentation misc Chemical technology
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title	Design and Validation of a Custom-Made Hyperspectral Microscope Imaging System for Biomedical Applications
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title_auth	Design and Validation of a Custom-Made Hyperspectral Microscope Imaging System for Biomedical Applications
abstract	Hyperspectral microscope imaging (HMI) is an emerging modality that integrates spatial information collected by standard laboratory microscopy and the spectral-based contrast obtained by hyperspectral imaging and may be instrumental in establishing novel quantitative diagnostic methodologies, particularly in histopathology. Further expansion of HMI capabilities hinges upon the modularity and versatility of systems and their proper standardization. In this report, we describe the design, calibration, characterization, and validation of the custom-made laboratory HMI system based on a Zeiss Axiotron fully motorized microscope and a custom-developed Czerny-Turner-type monochromator. For these important steps, we rely on a previously designed calibration protocol. Validation of the system demonstrates a performance comparable to classic spectrometry laboratory systems. We further demonstrate validation against a laboratory hyperspectral imaging system for macroscopic samples, enabling future comparison of spectral imaging results across length scales. An example of the utility of our custom-made HMI system on a standard hematoxylin and eosin-stained histology slide is also shown.
abstractGer	Hyperspectral microscope imaging (HMI) is an emerging modality that integrates spatial information collected by standard laboratory microscopy and the spectral-based contrast obtained by hyperspectral imaging and may be instrumental in establishing novel quantitative diagnostic methodologies, particularly in histopathology. Further expansion of HMI capabilities hinges upon the modularity and versatility of systems and their proper standardization. In this report, we describe the design, calibration, characterization, and validation of the custom-made laboratory HMI system based on a Zeiss Axiotron fully motorized microscope and a custom-developed Czerny-Turner-type monochromator. For these important steps, we rely on a previously designed calibration protocol. Validation of the system demonstrates a performance comparable to classic spectrometry laboratory systems. We further demonstrate validation against a laboratory hyperspectral imaging system for macroscopic samples, enabling future comparison of spectral imaging results across length scales. An example of the utility of our custom-made HMI system on a standard hematoxylin and eosin-stained histology slide is also shown.
abstract_unstemmed	Hyperspectral microscope imaging (HMI) is an emerging modality that integrates spatial information collected by standard laboratory microscopy and the spectral-based contrast obtained by hyperspectral imaging and may be instrumental in establishing novel quantitative diagnostic methodologies, particularly in histopathology. Further expansion of HMI capabilities hinges upon the modularity and versatility of systems and their proper standardization. In this report, we describe the design, calibration, characterization, and validation of the custom-made laboratory HMI system based on a Zeiss Axiotron fully motorized microscope and a custom-developed Czerny-Turner-type monochromator. For these important steps, we rely on a previously designed calibration protocol. Validation of the system demonstrates a performance comparable to classic spectrometry laboratory systems. We further demonstrate validation against a laboratory hyperspectral imaging system for macroscopic samples, enabling future comparison of spectral imaging results across length scales. An example of the utility of our custom-made HMI system on a standard hematoxylin and eosin-stained histology slide is also shown.
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title_short	Design and Validation of a Custom-Made Hyperspectral Microscope Imaging System for Biomedical Applications
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