Hydrogen/Deuterium Exchange Mass Spectrometry for Probing the Isomeric Forms of Oleocanthal and Oleacin in Extra Virgin Olive Oils

Reversed-phase liquid chromatography and electrospray ionization with Fourier-transform single and tandem mass spectrometry (RPLC-ESI-FTMS and FTMS/MS) were employed for the structural characterization of oleocanthal (OLEO) and oleacin (OLEA), two of the most important bioactive secoiridoids occurri...
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Autor*in:	Ramona Abbattista [verfasserIn] Ilario Losito [verfasserIn] Graziana Basile [verfasserIn] Andrea Castellaneta [verfasserIn] Giovanni Ventura [verfasserIn] Cosima Damiana Calvano [verfasserIn] Tommaso R. I. Cataldi [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	secoiridoids oleocanthal oleacin olive oil high resolution mass spectrometry H/D exchange

Übergeordnetes Werk:	In: Molecules - MDPI AG, 2003, 28(2023), 5, p 2066
Übergeordnetes Werk:	volume:28 ; year:2023 ; number:5, p 2066

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/molecules28052066

Katalog-ID:	DOAJ087989190

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520			\|a Reversed-phase liquid chromatography and electrospray ionization with Fourier-transform single and tandem mass spectrometry (RPLC-ESI-FTMS and FTMS/MS) were employed for the structural characterization of oleocanthal (OLEO) and oleacin (OLEA), two of the most important bioactive secoiridoids occurring in extra virgin olive oils (EVOOs). The existence of several isoforms of OLEO and OLEA was inferred from the chromatographic separation, accompanied, in the case of OLEA, by minor peaks due to oxidized OLEO recognized as oleocanthalic acid isoforms. The detailed analysis of the product ion tandem MS spectra of deprotonated molecules ([M-H]<sup<−</sup<) was unable to clarify the correlation between chromatographic peaks and specific OLEO/OLEA isoforms, including two types of predominant dialdehydic compounds, named <i<Open Forms II</i<, containing a double bond between carbon atoms C8 and C10, and a group of diasteroisomeric closed-structure (i.e., cyclic) isoforms, named <i<Closed Forms I</i<. This issue was addressed by H/D exchange (HDX) experiments on labile H atoms of OLEO and OLEA isoforms, performed using deuterated water as a co-solvent in the mobile phase. HDX unveiled the presence of stable di-enolic tautomers, in turn providing key evidence for the occurrence, as prevailing isoforms, of <i<Open Forms II</i< of OLEO and OLEA, different from those usually considered so far as the main isoforms of both secoiridoids (having a C=C bond between C8 and C9). It is expected that the new structural details inferred for the prevailing isoforms of OLEO and OLEA will help in understanding the remarkable bioactivity exhibited by the two compounds.
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author_variant	r a ra i l il g b gb a c ac g v gv c d c cdc t r i c tric
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allfields	10.3390/molecules28052066 doi (DE-627)DOAJ087989190 (DE-599)DOAJf716dc00ea16454fba7638c3993924a1 DE-627 ger DE-627 rakwb eng QD241-441 Ramona Abbattista verfasserin aut Hydrogen/Deuterium Exchange Mass Spectrometry for Probing the Isomeric Forms of Oleocanthal and Oleacin in Extra Virgin Olive Oils 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Reversed-phase liquid chromatography and electrospray ionization with Fourier-transform single and tandem mass spectrometry (RPLC-ESI-FTMS and FTMS/MS) were employed for the structural characterization of oleocanthal (OLEO) and oleacin (OLEA), two of the most important bioactive secoiridoids occurring in extra virgin olive oils (EVOOs). The existence of several isoforms of OLEO and OLEA was inferred from the chromatographic separation, accompanied, in the case of OLEA, by minor peaks due to oxidized OLEO recognized as oleocanthalic acid isoforms. The detailed analysis of the product ion tandem MS spectra of deprotonated molecules ([M-H]<sup<−</sup<) was unable to clarify the correlation between chromatographic peaks and specific OLEO/OLEA isoforms, including two types of predominant dialdehydic compounds, named <i<Open Forms II</i<, containing a double bond between carbon atoms C8 and C10, and a group of diasteroisomeric closed-structure (i.e., cyclic) isoforms, named <i<Closed Forms I</i<. This issue was addressed by H/D exchange (HDX) experiments on labile H atoms of OLEO and OLEA isoforms, performed using deuterated water as a co-solvent in the mobile phase. HDX unveiled the presence of stable di-enolic tautomers, in turn providing key evidence for the occurrence, as prevailing isoforms, of <i<Open Forms II</i< of OLEO and OLEA, different from those usually considered so far as the main isoforms of both secoiridoids (having a C=C bond between C8 and C9). It is expected that the new structural details inferred for the prevailing isoforms of OLEO and OLEA will help in understanding the remarkable bioactivity exhibited by the two compounds. secoiridoids oleocanthal oleacin olive oil high resolution mass spectrometry H/D exchange Organic chemistry Ilario Losito verfasserin aut Graziana Basile verfasserin aut Andrea Castellaneta verfasserin aut Giovanni Ventura verfasserin aut Cosima Damiana Calvano verfasserin aut Tommaso R. I. Cataldi verfasserin aut In Molecules MDPI AG, 2003 28(2023), 5, p 2066 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:28 year:2023 number:5, p 2066 https://doi.org/10.3390/molecules28052066 kostenfrei https://doaj.org/article/f716dc00ea16454fba7638c3993924a1 kostenfrei https://www.mdpi.com/1420-3049/28/5/2066 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2023 5, p 2066
spelling	10.3390/molecules28052066 doi (DE-627)DOAJ087989190 (DE-599)DOAJf716dc00ea16454fba7638c3993924a1 DE-627 ger DE-627 rakwb eng QD241-441 Ramona Abbattista verfasserin aut Hydrogen/Deuterium Exchange Mass Spectrometry for Probing the Isomeric Forms of Oleocanthal and Oleacin in Extra Virgin Olive Oils 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Reversed-phase liquid chromatography and electrospray ionization with Fourier-transform single and tandem mass spectrometry (RPLC-ESI-FTMS and FTMS/MS) were employed for the structural characterization of oleocanthal (OLEO) and oleacin (OLEA), two of the most important bioactive secoiridoids occurring in extra virgin olive oils (EVOOs). The existence of several isoforms of OLEO and OLEA was inferred from the chromatographic separation, accompanied, in the case of OLEA, by minor peaks due to oxidized OLEO recognized as oleocanthalic acid isoforms. The detailed analysis of the product ion tandem MS spectra of deprotonated molecules ([M-H]<sup<−</sup<) was unable to clarify the correlation between chromatographic peaks and specific OLEO/OLEA isoforms, including two types of predominant dialdehydic compounds, named <i<Open Forms II</i<, containing a double bond between carbon atoms C8 and C10, and a group of diasteroisomeric closed-structure (i.e., cyclic) isoforms, named <i<Closed Forms I</i<. This issue was addressed by H/D exchange (HDX) experiments on labile H atoms of OLEO and OLEA isoforms, performed using deuterated water as a co-solvent in the mobile phase. HDX unveiled the presence of stable di-enolic tautomers, in turn providing key evidence for the occurrence, as prevailing isoforms, of <i<Open Forms II</i< of OLEO and OLEA, different from those usually considered so far as the main isoforms of both secoiridoids (having a C=C bond between C8 and C9). It is expected that the new structural details inferred for the prevailing isoforms of OLEO and OLEA will help in understanding the remarkable bioactivity exhibited by the two compounds. secoiridoids oleocanthal oleacin olive oil high resolution mass spectrometry H/D exchange Organic chemistry Ilario Losito verfasserin aut Graziana Basile verfasserin aut Andrea Castellaneta verfasserin aut Giovanni Ventura verfasserin aut Cosima Damiana Calvano verfasserin aut Tommaso R. I. Cataldi verfasserin aut In Molecules MDPI AG, 2003 28(2023), 5, p 2066 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:28 year:2023 number:5, p 2066 https://doi.org/10.3390/molecules28052066 kostenfrei https://doaj.org/article/f716dc00ea16454fba7638c3993924a1 kostenfrei https://www.mdpi.com/1420-3049/28/5/2066 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2023 5, p 2066
allfields_unstemmed	10.3390/molecules28052066 doi (DE-627)DOAJ087989190 (DE-599)DOAJf716dc00ea16454fba7638c3993924a1 DE-627 ger DE-627 rakwb eng QD241-441 Ramona Abbattista verfasserin aut Hydrogen/Deuterium Exchange Mass Spectrometry for Probing the Isomeric Forms of Oleocanthal and Oleacin in Extra Virgin Olive Oils 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Reversed-phase liquid chromatography and electrospray ionization with Fourier-transform single and tandem mass spectrometry (RPLC-ESI-FTMS and FTMS/MS) were employed for the structural characterization of oleocanthal (OLEO) and oleacin (OLEA), two of the most important bioactive secoiridoids occurring in extra virgin olive oils (EVOOs). The existence of several isoforms of OLEO and OLEA was inferred from the chromatographic separation, accompanied, in the case of OLEA, by minor peaks due to oxidized OLEO recognized as oleocanthalic acid isoforms. The detailed analysis of the product ion tandem MS spectra of deprotonated molecules ([M-H]<sup<−</sup<) was unable to clarify the correlation between chromatographic peaks and specific OLEO/OLEA isoforms, including two types of predominant dialdehydic compounds, named <i<Open Forms II</i<, containing a double bond between carbon atoms C8 and C10, and a group of diasteroisomeric closed-structure (i.e., cyclic) isoforms, named <i<Closed Forms I</i<. This issue was addressed by H/D exchange (HDX) experiments on labile H atoms of OLEO and OLEA isoforms, performed using deuterated water as a co-solvent in the mobile phase. HDX unveiled the presence of stable di-enolic tautomers, in turn providing key evidence for the occurrence, as prevailing isoforms, of <i<Open Forms II</i< of OLEO and OLEA, different from those usually considered so far as the main isoforms of both secoiridoids (having a C=C bond between C8 and C9). It is expected that the new structural details inferred for the prevailing isoforms of OLEO and OLEA will help in understanding the remarkable bioactivity exhibited by the two compounds. secoiridoids oleocanthal oleacin olive oil high resolution mass spectrometry H/D exchange Organic chemistry Ilario Losito verfasserin aut Graziana Basile verfasserin aut Andrea Castellaneta verfasserin aut Giovanni Ventura verfasserin aut Cosima Damiana Calvano verfasserin aut Tommaso R. I. Cataldi verfasserin aut In Molecules MDPI AG, 2003 28(2023), 5, p 2066 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:28 year:2023 number:5, p 2066 https://doi.org/10.3390/molecules28052066 kostenfrei https://doaj.org/article/f716dc00ea16454fba7638c3993924a1 kostenfrei https://www.mdpi.com/1420-3049/28/5/2066 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2023 5, p 2066
allfieldsGer	10.3390/molecules28052066 doi (DE-627)DOAJ087989190 (DE-599)DOAJf716dc00ea16454fba7638c3993924a1 DE-627 ger DE-627 rakwb eng QD241-441 Ramona Abbattista verfasserin aut Hydrogen/Deuterium Exchange Mass Spectrometry for Probing the Isomeric Forms of Oleocanthal and Oleacin in Extra Virgin Olive Oils 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Reversed-phase liquid chromatography and electrospray ionization with Fourier-transform single and tandem mass spectrometry (RPLC-ESI-FTMS and FTMS/MS) were employed for the structural characterization of oleocanthal (OLEO) and oleacin (OLEA), two of the most important bioactive secoiridoids occurring in extra virgin olive oils (EVOOs). The existence of several isoforms of OLEO and OLEA was inferred from the chromatographic separation, accompanied, in the case of OLEA, by minor peaks due to oxidized OLEO recognized as oleocanthalic acid isoforms. The detailed analysis of the product ion tandem MS spectra of deprotonated molecules ([M-H]<sup<−</sup<) was unable to clarify the correlation between chromatographic peaks and specific OLEO/OLEA isoforms, including two types of predominant dialdehydic compounds, named <i<Open Forms II</i<, containing a double bond between carbon atoms C8 and C10, and a group of diasteroisomeric closed-structure (i.e., cyclic) isoforms, named <i<Closed Forms I</i<. This issue was addressed by H/D exchange (HDX) experiments on labile H atoms of OLEO and OLEA isoforms, performed using deuterated water as a co-solvent in the mobile phase. HDX unveiled the presence of stable di-enolic tautomers, in turn providing key evidence for the occurrence, as prevailing isoforms, of <i<Open Forms II</i< of OLEO and OLEA, different from those usually considered so far as the main isoforms of both secoiridoids (having a C=C bond between C8 and C9). It is expected that the new structural details inferred for the prevailing isoforms of OLEO and OLEA will help in understanding the remarkable bioactivity exhibited by the two compounds. secoiridoids oleocanthal oleacin olive oil high resolution mass spectrometry H/D exchange Organic chemistry Ilario Losito verfasserin aut Graziana Basile verfasserin aut Andrea Castellaneta verfasserin aut Giovanni Ventura verfasserin aut Cosima Damiana Calvano verfasserin aut Tommaso R. I. Cataldi verfasserin aut In Molecules MDPI AG, 2003 28(2023), 5, p 2066 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:28 year:2023 number:5, p 2066 https://doi.org/10.3390/molecules28052066 kostenfrei https://doaj.org/article/f716dc00ea16454fba7638c3993924a1 kostenfrei https://www.mdpi.com/1420-3049/28/5/2066 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2023 5, p 2066
allfieldsSound	10.3390/molecules28052066 doi (DE-627)DOAJ087989190 (DE-599)DOAJf716dc00ea16454fba7638c3993924a1 DE-627 ger DE-627 rakwb eng QD241-441 Ramona Abbattista verfasserin aut Hydrogen/Deuterium Exchange Mass Spectrometry for Probing the Isomeric Forms of Oleocanthal and Oleacin in Extra Virgin Olive Oils 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Reversed-phase liquid chromatography and electrospray ionization with Fourier-transform single and tandem mass spectrometry (RPLC-ESI-FTMS and FTMS/MS) were employed for the structural characterization of oleocanthal (OLEO) and oleacin (OLEA), two of the most important bioactive secoiridoids occurring in extra virgin olive oils (EVOOs). The existence of several isoforms of OLEO and OLEA was inferred from the chromatographic separation, accompanied, in the case of OLEA, by minor peaks due to oxidized OLEO recognized as oleocanthalic acid isoforms. The detailed analysis of the product ion tandem MS spectra of deprotonated molecules ([M-H]<sup<−</sup<) was unable to clarify the correlation between chromatographic peaks and specific OLEO/OLEA isoforms, including two types of predominant dialdehydic compounds, named <i<Open Forms II</i<, containing a double bond between carbon atoms C8 and C10, and a group of diasteroisomeric closed-structure (i.e., cyclic) isoforms, named <i<Closed Forms I</i<. This issue was addressed by H/D exchange (HDX) experiments on labile H atoms of OLEO and OLEA isoforms, performed using deuterated water as a co-solvent in the mobile phase. HDX unveiled the presence of stable di-enolic tautomers, in turn providing key evidence for the occurrence, as prevailing isoforms, of <i<Open Forms II</i< of OLEO and OLEA, different from those usually considered so far as the main isoforms of both secoiridoids (having a C=C bond between C8 and C9). It is expected that the new structural details inferred for the prevailing isoforms of OLEO and OLEA will help in understanding the remarkable bioactivity exhibited by the two compounds. secoiridoids oleocanthal oleacin olive oil high resolution mass spectrometry H/D exchange Organic chemistry Ilario Losito verfasserin aut Graziana Basile verfasserin aut Andrea Castellaneta verfasserin aut Giovanni Ventura verfasserin aut Cosima Damiana Calvano verfasserin aut Tommaso R. I. Cataldi verfasserin aut In Molecules MDPI AG, 2003 28(2023), 5, p 2066 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:28 year:2023 number:5, p 2066 https://doi.org/10.3390/molecules28052066 kostenfrei https://doaj.org/article/f716dc00ea16454fba7638c3993924a1 kostenfrei https://www.mdpi.com/1420-3049/28/5/2066 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2023 5, p 2066
language	English
source	In Molecules 28(2023), 5, p 2066 volume:28 year:2023 number:5, p 2066
sourceStr	In Molecules 28(2023), 5, p 2066 volume:28 year:2023 number:5, p 2066
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	secoiridoids oleocanthal oleacin olive oil high resolution mass spectrometry H/D exchange Organic chemistry
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container_title	Molecules
authorswithroles_txt_mv	Ramona Abbattista @@aut@@ Ilario Losito @@aut@@ Graziana Basile @@aut@@ Andrea Castellaneta @@aut@@ Giovanni Ventura @@aut@@ Cosima Damiana Calvano @@aut@@ Tommaso R. I. Cataldi @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
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author	Ramona Abbattista
spellingShingle	Ramona Abbattista misc QD241-441 misc secoiridoids misc oleocanthal misc oleacin misc olive oil misc high resolution mass spectrometry misc H/D exchange misc Organic chemistry Hydrogen/Deuterium Exchange Mass Spectrometry for Probing the Isomeric Forms of Oleocanthal and Oleacin in Extra Virgin Olive Oils
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issn	14203049
topic_title	QD241-441 Hydrogen/Deuterium Exchange Mass Spectrometry for Probing the Isomeric Forms of Oleocanthal and Oleacin in Extra Virgin Olive Oils secoiridoids oleocanthal oleacin olive oil high resolution mass spectrometry H/D exchange
topic	misc QD241-441 misc secoiridoids misc oleocanthal misc oleacin misc olive oil misc high resolution mass spectrometry misc H/D exchange misc Organic chemistry
topic_unstemmed	misc QD241-441 misc secoiridoids misc oleocanthal misc oleacin misc olive oil misc high resolution mass spectrometry misc H/D exchange misc Organic chemistry
topic_browse	misc QD241-441 misc secoiridoids misc oleocanthal misc oleacin misc olive oil misc high resolution mass spectrometry misc H/D exchange misc Organic chemistry
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title	Hydrogen/Deuterium Exchange Mass Spectrometry for Probing the Isomeric Forms of Oleocanthal and Oleacin in Extra Virgin Olive Oils
ctrlnum	(DE-627)DOAJ087989190 (DE-599)DOAJf716dc00ea16454fba7638c3993924a1
title_full	Hydrogen/Deuterium Exchange Mass Spectrometry for Probing the Isomeric Forms of Oleocanthal and Oleacin in Extra Virgin Olive Oils
author_sort	Ramona Abbattista
journal	Molecules
journalStr	Molecules
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author_browse	Ramona Abbattista Ilario Losito Graziana Basile Andrea Castellaneta Giovanni Ventura Cosima Damiana Calvano Tommaso R. I. Cataldi
container_volume	28
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author-letter	Ramona Abbattista
doi_str_mv	10.3390/molecules28052066
author2-role	verfasserin
title_sort	hydrogen/deuterium exchange mass spectrometry for probing the isomeric forms of oleocanthal and oleacin in extra virgin olive oils
callnumber	QD241-441
title_auth	Hydrogen/Deuterium Exchange Mass Spectrometry for Probing the Isomeric Forms of Oleocanthal and Oleacin in Extra Virgin Olive Oils
abstract	Reversed-phase liquid chromatography and electrospray ionization with Fourier-transform single and tandem mass spectrometry (RPLC-ESI-FTMS and FTMS/MS) were employed for the structural characterization of oleocanthal (OLEO) and oleacin (OLEA), two of the most important bioactive secoiridoids occurring in extra virgin olive oils (EVOOs). The existence of several isoforms of OLEO and OLEA was inferred from the chromatographic separation, accompanied, in the case of OLEA, by minor peaks due to oxidized OLEO recognized as oleocanthalic acid isoforms. The detailed analysis of the product ion tandem MS spectra of deprotonated molecules ([M-H]<sup<−</sup<) was unable to clarify the correlation between chromatographic peaks and specific OLEO/OLEA isoforms, including two types of predominant dialdehydic compounds, named <i<Open Forms II</i<, containing a double bond between carbon atoms C8 and C10, and a group of diasteroisomeric closed-structure (i.e., cyclic) isoforms, named <i<Closed Forms I</i<. This issue was addressed by H/D exchange (HDX) experiments on labile H atoms of OLEO and OLEA isoforms, performed using deuterated water as a co-solvent in the mobile phase. HDX unveiled the presence of stable di-enolic tautomers, in turn providing key evidence for the occurrence, as prevailing isoforms, of <i<Open Forms II</i< of OLEO and OLEA, different from those usually considered so far as the main isoforms of both secoiridoids (having a C=C bond between C8 and C9). It is expected that the new structural details inferred for the prevailing isoforms of OLEO and OLEA will help in understanding the remarkable bioactivity exhibited by the two compounds.
abstractGer	Reversed-phase liquid chromatography and electrospray ionization with Fourier-transform single and tandem mass spectrometry (RPLC-ESI-FTMS and FTMS/MS) were employed for the structural characterization of oleocanthal (OLEO) and oleacin (OLEA), two of the most important bioactive secoiridoids occurring in extra virgin olive oils (EVOOs). The existence of several isoforms of OLEO and OLEA was inferred from the chromatographic separation, accompanied, in the case of OLEA, by minor peaks due to oxidized OLEO recognized as oleocanthalic acid isoforms. The detailed analysis of the product ion tandem MS spectra of deprotonated molecules ([M-H]<sup<−</sup<) was unable to clarify the correlation between chromatographic peaks and specific OLEO/OLEA isoforms, including two types of predominant dialdehydic compounds, named <i<Open Forms II</i<, containing a double bond between carbon atoms C8 and C10, and a group of diasteroisomeric closed-structure (i.e., cyclic) isoforms, named <i<Closed Forms I</i<. This issue was addressed by H/D exchange (HDX) experiments on labile H atoms of OLEO and OLEA isoforms, performed using deuterated water as a co-solvent in the mobile phase. HDX unveiled the presence of stable di-enolic tautomers, in turn providing key evidence for the occurrence, as prevailing isoforms, of <i<Open Forms II</i< of OLEO and OLEA, different from those usually considered so far as the main isoforms of both secoiridoids (having a C=C bond between C8 and C9). It is expected that the new structural details inferred for the prevailing isoforms of OLEO and OLEA will help in understanding the remarkable bioactivity exhibited by the two compounds.
abstract_unstemmed	Reversed-phase liquid chromatography and electrospray ionization with Fourier-transform single and tandem mass spectrometry (RPLC-ESI-FTMS and FTMS/MS) were employed for the structural characterization of oleocanthal (OLEO) and oleacin (OLEA), two of the most important bioactive secoiridoids occurring in extra virgin olive oils (EVOOs). The existence of several isoforms of OLEO and OLEA was inferred from the chromatographic separation, accompanied, in the case of OLEA, by minor peaks due to oxidized OLEO recognized as oleocanthalic acid isoforms. The detailed analysis of the product ion tandem MS spectra of deprotonated molecules ([M-H]<sup<−</sup<) was unable to clarify the correlation between chromatographic peaks and specific OLEO/OLEA isoforms, including two types of predominant dialdehydic compounds, named <i<Open Forms II</i<, containing a double bond between carbon atoms C8 and C10, and a group of diasteroisomeric closed-structure (i.e., cyclic) isoforms, named <i<Closed Forms I</i<. This issue was addressed by H/D exchange (HDX) experiments on labile H atoms of OLEO and OLEA isoforms, performed using deuterated water as a co-solvent in the mobile phase. HDX unveiled the presence of stable di-enolic tautomers, in turn providing key evidence for the occurrence, as prevailing isoforms, of <i<Open Forms II</i< of OLEO and OLEA, different from those usually considered so far as the main isoforms of both secoiridoids (having a C=C bond between C8 and C9). It is expected that the new structural details inferred for the prevailing isoforms of OLEO and OLEA will help in understanding the remarkable bioactivity exhibited by the two compounds.
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container_issue	5, p 2066
title_short	Hydrogen/Deuterium Exchange Mass Spectrometry for Probing the Isomeric Forms of Oleocanthal and Oleacin in Extra Virgin Olive Oils
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