<i<CaSPDS</i<, a Spermidine Synthase Gene from Pepper (<i<Capsicum annuum</i< L.), Plays an Important Role in Response to Cold Stress

Spermidine synthase (SPDS) is a key enzyme in the polyamine anabolic pathway. <i<SPDS</i< genes help regulate plant response to environmental stresses, but their roles in pepper remain unclear. In this study, we identified and cloned a SPDS gene from pepper (<i<Capsicum annuum</...
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Autor*in:	Jianwei Zhang [verfasserIn] Minghui Xie [verfasserIn] Guofeng Yu [verfasserIn] Dong Wang [verfasserIn] Zeping Xu [verfasserIn] Le Liang [verfasserIn] Jiachang Xiao [verfasserIn] Yongdong Xie [verfasserIn] Yi Tang [verfasserIn] Guochao Sun [verfasserIn] Bo Sun [verfasserIn] Zhi Huang [verfasserIn] Yunsong Lai [verfasserIn] Huanxiu Li [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	spermidine synthase virus-induced gene silencing overexpression cold stress pepper

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 24(2023), 5, p 5013
Übergeordnetes Werk:	volume:24 ; year:2023 ; number:5, p 5013

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms24055013

Katalog-ID:	DOAJ088000133

Internformat


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520			\|a Spermidine synthase (SPDS) is a key enzyme in the polyamine anabolic pathway. <i<SPDS</i< genes help regulate plant response to environmental stresses, but their roles in pepper remain unclear. In this study, we identified and cloned a SPDS gene from pepper (<i<Capsicum annuum</i< L.), named <i<CaSPDS</i< (LOC107847831). Bioinformatics analysis indicated that <i<CaSPDS</i< contains two highly conserved domains: an SPDS tetramerisation domain and a spermine/SPDS domain. Quantitative reverse-transcription polymerase chain reaction results showed that <i<CaSPDS</i< was highly expressed in the stems, flowers, and mature fruits of pepper and was rapidly induced by cold stress. The function of <i<CaSPDS</i< in cold stress response was studied by silencing and overexpressing it in pepper and <i<Arabidopsis</i<, respectively. Cold injury was more serious and reactive oxygen species levels were greater in the <i<CaSPDS</i<-silenced seedlings than in the wild-type (WT) seedlings after cold treatment. Compared with the WT plants, the <i<CaSPDS</i<-overexpression <i<Arabidopsis</i< plants were more tolerant to cold stress and showed higher antioxidant enzyme activities, spermidine content, and cold-responsive gene (<i<AtCOR15A</i<, <i<AtRD29A</i<, <i<AtCOR47</i<, and <i<AtKIN1</i<) expression. These results indicate that <i<CaSPDS</i< plays important roles in cold stress response and is valuable in molecular breeding to enhance the cold tolerance of pepper.
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allfields	10.3390/ijms24055013 doi (DE-627)DOAJ088000133 (DE-599)DOAJ184b6d28193e41919d72b51cbe49fcd0 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Jianwei Zhang verfasserin aut <i<CaSPDS</i<, a Spermidine Synthase Gene from Pepper (<i<Capsicum annuum</i< L.), Plays an Important Role in Response to Cold Stress 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Spermidine synthase (SPDS) is a key enzyme in the polyamine anabolic pathway. <i<SPDS</i< genes help regulate plant response to environmental stresses, but their roles in pepper remain unclear. In this study, we identified and cloned a SPDS gene from pepper (<i<Capsicum annuum</i< L.), named <i<CaSPDS</i< (LOC107847831). Bioinformatics analysis indicated that <i<CaSPDS</i< contains two highly conserved domains: an SPDS tetramerisation domain and a spermine/SPDS domain. Quantitative reverse-transcription polymerase chain reaction results showed that <i<CaSPDS</i< was highly expressed in the stems, flowers, and mature fruits of pepper and was rapidly induced by cold stress. The function of <i<CaSPDS</i< in cold stress response was studied by silencing and overexpressing it in pepper and <i<Arabidopsis</i<, respectively. Cold injury was more serious and reactive oxygen species levels were greater in the <i<CaSPDS</i<-silenced seedlings than in the wild-type (WT) seedlings after cold treatment. Compared with the WT plants, the <i<CaSPDS</i<-overexpression <i<Arabidopsis</i< plants were more tolerant to cold stress and showed higher antioxidant enzyme activities, spermidine content, and cold-responsive gene (<i<AtCOR15A</i<, <i<AtRD29A</i<, <i<AtCOR47</i<, and <i<AtKIN1</i<) expression. These results indicate that <i<CaSPDS</i< plays important roles in cold stress response and is valuable in molecular breeding to enhance the cold tolerance of pepper. spermidine synthase virus-induced gene silencing overexpression cold stress pepper Biology (General) Chemistry Minghui Xie verfasserin aut Guofeng Yu verfasserin aut Dong Wang verfasserin aut Zeping Xu verfasserin aut Le Liang verfasserin aut Jiachang Xiao verfasserin aut Yongdong Xie verfasserin aut Yi Tang verfasserin aut Guochao Sun verfasserin aut Bo Sun verfasserin aut Zhi Huang verfasserin aut Yunsong Lai verfasserin aut Huanxiu Li verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 5, p 5013 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:5, p 5013 https://doi.org/10.3390/ijms24055013 kostenfrei https://doaj.org/article/184b6d28193e41919d72b51cbe49fcd0 kostenfrei https://www.mdpi.com/1422-0067/24/5/5013 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 5, p 5013
spelling	10.3390/ijms24055013 doi (DE-627)DOAJ088000133 (DE-599)DOAJ184b6d28193e41919d72b51cbe49fcd0 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Jianwei Zhang verfasserin aut <i<CaSPDS</i<, a Spermidine Synthase Gene from Pepper (<i<Capsicum annuum</i< L.), Plays an Important Role in Response to Cold Stress 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Spermidine synthase (SPDS) is a key enzyme in the polyamine anabolic pathway. <i<SPDS</i< genes help regulate plant response to environmental stresses, but their roles in pepper remain unclear. In this study, we identified and cloned a SPDS gene from pepper (<i<Capsicum annuum</i< L.), named <i<CaSPDS</i< (LOC107847831). Bioinformatics analysis indicated that <i<CaSPDS</i< contains two highly conserved domains: an SPDS tetramerisation domain and a spermine/SPDS domain. Quantitative reverse-transcription polymerase chain reaction results showed that <i<CaSPDS</i< was highly expressed in the stems, flowers, and mature fruits of pepper and was rapidly induced by cold stress. The function of <i<CaSPDS</i< in cold stress response was studied by silencing and overexpressing it in pepper and <i<Arabidopsis</i<, respectively. Cold injury was more serious and reactive oxygen species levels were greater in the <i<CaSPDS</i<-silenced seedlings than in the wild-type (WT) seedlings after cold treatment. Compared with the WT plants, the <i<CaSPDS</i<-overexpression <i<Arabidopsis</i< plants were more tolerant to cold stress and showed higher antioxidant enzyme activities, spermidine content, and cold-responsive gene (<i<AtCOR15A</i<, <i<AtRD29A</i<, <i<AtCOR47</i<, and <i<AtKIN1</i<) expression. These results indicate that <i<CaSPDS</i< plays important roles in cold stress response and is valuable in molecular breeding to enhance the cold tolerance of pepper. spermidine synthase virus-induced gene silencing overexpression cold stress pepper Biology (General) Chemistry Minghui Xie verfasserin aut Guofeng Yu verfasserin aut Dong Wang verfasserin aut Zeping Xu verfasserin aut Le Liang verfasserin aut Jiachang Xiao verfasserin aut Yongdong Xie verfasserin aut Yi Tang verfasserin aut Guochao Sun verfasserin aut Bo Sun verfasserin aut Zhi Huang verfasserin aut Yunsong Lai verfasserin aut Huanxiu Li verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 5, p 5013 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:5, p 5013 https://doi.org/10.3390/ijms24055013 kostenfrei https://doaj.org/article/184b6d28193e41919d72b51cbe49fcd0 kostenfrei https://www.mdpi.com/1422-0067/24/5/5013 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 5, p 5013
allfields_unstemmed	10.3390/ijms24055013 doi (DE-627)DOAJ088000133 (DE-599)DOAJ184b6d28193e41919d72b51cbe49fcd0 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Jianwei Zhang verfasserin aut <i<CaSPDS</i<, a Spermidine Synthase Gene from Pepper (<i<Capsicum annuum</i< L.), Plays an Important Role in Response to Cold Stress 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Spermidine synthase (SPDS) is a key enzyme in the polyamine anabolic pathway. <i<SPDS</i< genes help regulate plant response to environmental stresses, but their roles in pepper remain unclear. In this study, we identified and cloned a SPDS gene from pepper (<i<Capsicum annuum</i< L.), named <i<CaSPDS</i< (LOC107847831). Bioinformatics analysis indicated that <i<CaSPDS</i< contains two highly conserved domains: an SPDS tetramerisation domain and a spermine/SPDS domain. Quantitative reverse-transcription polymerase chain reaction results showed that <i<CaSPDS</i< was highly expressed in the stems, flowers, and mature fruits of pepper and was rapidly induced by cold stress. The function of <i<CaSPDS</i< in cold stress response was studied by silencing and overexpressing it in pepper and <i<Arabidopsis</i<, respectively. Cold injury was more serious and reactive oxygen species levels were greater in the <i<CaSPDS</i<-silenced seedlings than in the wild-type (WT) seedlings after cold treatment. Compared with the WT plants, the <i<CaSPDS</i<-overexpression <i<Arabidopsis</i< plants were more tolerant to cold stress and showed higher antioxidant enzyme activities, spermidine content, and cold-responsive gene (<i<AtCOR15A</i<, <i<AtRD29A</i<, <i<AtCOR47</i<, and <i<AtKIN1</i<) expression. These results indicate that <i<CaSPDS</i< plays important roles in cold stress response and is valuable in molecular breeding to enhance the cold tolerance of pepper. spermidine synthase virus-induced gene silencing overexpression cold stress pepper Biology (General) Chemistry Minghui Xie verfasserin aut Guofeng Yu verfasserin aut Dong Wang verfasserin aut Zeping Xu verfasserin aut Le Liang verfasserin aut Jiachang Xiao verfasserin aut Yongdong Xie verfasserin aut Yi Tang verfasserin aut Guochao Sun verfasserin aut Bo Sun verfasserin aut Zhi Huang verfasserin aut Yunsong Lai verfasserin aut Huanxiu Li verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 5, p 5013 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:5, p 5013 https://doi.org/10.3390/ijms24055013 kostenfrei https://doaj.org/article/184b6d28193e41919d72b51cbe49fcd0 kostenfrei https://www.mdpi.com/1422-0067/24/5/5013 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 5, p 5013
allfieldsGer	10.3390/ijms24055013 doi (DE-627)DOAJ088000133 (DE-599)DOAJ184b6d28193e41919d72b51cbe49fcd0 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Jianwei Zhang verfasserin aut <i<CaSPDS</i<, a Spermidine Synthase Gene from Pepper (<i<Capsicum annuum</i< L.), Plays an Important Role in Response to Cold Stress 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Spermidine synthase (SPDS) is a key enzyme in the polyamine anabolic pathway. <i<SPDS</i< genes help regulate plant response to environmental stresses, but their roles in pepper remain unclear. In this study, we identified and cloned a SPDS gene from pepper (<i<Capsicum annuum</i< L.), named <i<CaSPDS</i< (LOC107847831). Bioinformatics analysis indicated that <i<CaSPDS</i< contains two highly conserved domains: an SPDS tetramerisation domain and a spermine/SPDS domain. Quantitative reverse-transcription polymerase chain reaction results showed that <i<CaSPDS</i< was highly expressed in the stems, flowers, and mature fruits of pepper and was rapidly induced by cold stress. The function of <i<CaSPDS</i< in cold stress response was studied by silencing and overexpressing it in pepper and <i<Arabidopsis</i<, respectively. Cold injury was more serious and reactive oxygen species levels were greater in the <i<CaSPDS</i<-silenced seedlings than in the wild-type (WT) seedlings after cold treatment. Compared with the WT plants, the <i<CaSPDS</i<-overexpression <i<Arabidopsis</i< plants were more tolerant to cold stress and showed higher antioxidant enzyme activities, spermidine content, and cold-responsive gene (<i<AtCOR15A</i<, <i<AtRD29A</i<, <i<AtCOR47</i<, and <i<AtKIN1</i<) expression. These results indicate that <i<CaSPDS</i< plays important roles in cold stress response and is valuable in molecular breeding to enhance the cold tolerance of pepper. spermidine synthase virus-induced gene silencing overexpression cold stress pepper Biology (General) Chemistry Minghui Xie verfasserin aut Guofeng Yu verfasserin aut Dong Wang verfasserin aut Zeping Xu verfasserin aut Le Liang verfasserin aut Jiachang Xiao verfasserin aut Yongdong Xie verfasserin aut Yi Tang verfasserin aut Guochao Sun verfasserin aut Bo Sun verfasserin aut Zhi Huang verfasserin aut Yunsong Lai verfasserin aut Huanxiu Li verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 5, p 5013 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:5, p 5013 https://doi.org/10.3390/ijms24055013 kostenfrei https://doaj.org/article/184b6d28193e41919d72b51cbe49fcd0 kostenfrei https://www.mdpi.com/1422-0067/24/5/5013 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 5, p 5013
allfieldsSound	10.3390/ijms24055013 doi (DE-627)DOAJ088000133 (DE-599)DOAJ184b6d28193e41919d72b51cbe49fcd0 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Jianwei Zhang verfasserin aut <i<CaSPDS</i<, a Spermidine Synthase Gene from Pepper (<i<Capsicum annuum</i< L.), Plays an Important Role in Response to Cold Stress 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Spermidine synthase (SPDS) is a key enzyme in the polyamine anabolic pathway. <i<SPDS</i< genes help regulate plant response to environmental stresses, but their roles in pepper remain unclear. In this study, we identified and cloned a SPDS gene from pepper (<i<Capsicum annuum</i< L.), named <i<CaSPDS</i< (LOC107847831). Bioinformatics analysis indicated that <i<CaSPDS</i< contains two highly conserved domains: an SPDS tetramerisation domain and a spermine/SPDS domain. Quantitative reverse-transcription polymerase chain reaction results showed that <i<CaSPDS</i< was highly expressed in the stems, flowers, and mature fruits of pepper and was rapidly induced by cold stress. The function of <i<CaSPDS</i< in cold stress response was studied by silencing and overexpressing it in pepper and <i<Arabidopsis</i<, respectively. Cold injury was more serious and reactive oxygen species levels were greater in the <i<CaSPDS</i<-silenced seedlings than in the wild-type (WT) seedlings after cold treatment. Compared with the WT plants, the <i<CaSPDS</i<-overexpression <i<Arabidopsis</i< plants were more tolerant to cold stress and showed higher antioxidant enzyme activities, spermidine content, and cold-responsive gene (<i<AtCOR15A</i<, <i<AtRD29A</i<, <i<AtCOR47</i<, and <i<AtKIN1</i<) expression. These results indicate that <i<CaSPDS</i< plays important roles in cold stress response and is valuable in molecular breeding to enhance the cold tolerance of pepper. spermidine synthase virus-induced gene silencing overexpression cold stress pepper Biology (General) Chemistry Minghui Xie verfasserin aut Guofeng Yu verfasserin aut Dong Wang verfasserin aut Zeping Xu verfasserin aut Le Liang verfasserin aut Jiachang Xiao verfasserin aut Yongdong Xie verfasserin aut Yi Tang verfasserin aut Guochao Sun verfasserin aut Bo Sun verfasserin aut Zhi Huang verfasserin aut Yunsong Lai verfasserin aut Huanxiu Li verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 5, p 5013 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:5, p 5013 https://doi.org/10.3390/ijms24055013 kostenfrei https://doaj.org/article/184b6d28193e41919d72b51cbe49fcd0 kostenfrei https://www.mdpi.com/1422-0067/24/5/5013 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 5, p 5013
language	English
source	In International Journal of Molecular Sciences 24(2023), 5, p 5013 volume:24 year:2023 number:5, p 5013
sourceStr	In International Journal of Molecular Sciences 24(2023), 5, p 5013 volume:24 year:2023 number:5, p 5013
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	spermidine synthase virus-induced gene silencing overexpression cold stress pepper Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Jianwei Zhang @@aut@@ Minghui Xie @@aut@@ Guofeng Yu @@aut@@ Dong Wang @@aut@@ Zeping Xu @@aut@@ Le Liang @@aut@@ Jiachang Xiao @@aut@@ Yongdong Xie @@aut@@ Yi Tang @@aut@@ Guochao Sun @@aut@@ Bo Sun @@aut@@ Zhi Huang @@aut@@ Yunsong Lai @@aut@@ Huanxiu Li @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ088000133
language_de	englisch
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callnumber-first	Q - Science
author	Jianwei Zhang
spellingShingle	Jianwei Zhang misc QH301-705.5 misc QD1-999 misc spermidine synthase misc virus-induced gene silencing misc overexpression misc cold stress misc pepper misc Biology (General) misc Chemistry <i<CaSPDS</i<, a Spermidine Synthase Gene from Pepper (<i<Capsicum annuum</i< L.), Plays an Important Role in Response to Cold Stress
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topic_title	QH301-705.5 QD1-999 <i<CaSPDS</i<, a Spermidine Synthase Gene from Pepper (<i<Capsicum annuum</i< L.), Plays an Important Role in Response to Cold Stress spermidine synthase virus-induced gene silencing overexpression cold stress pepper
topic	misc QH301-705.5 misc QD1-999 misc spermidine synthase misc virus-induced gene silencing misc overexpression misc cold stress misc pepper misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc spermidine synthase misc virus-induced gene silencing misc overexpression misc cold stress misc pepper misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc spermidine synthase misc virus-induced gene silencing misc overexpression misc cold stress misc pepper misc Biology (General) misc Chemistry
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title	<i<CaSPDS</i<, a Spermidine Synthase Gene from Pepper (<i<Capsicum annuum</i< L.), Plays an Important Role in Response to Cold Stress
ctrlnum	(DE-627)DOAJ088000133 (DE-599)DOAJ184b6d28193e41919d72b51cbe49fcd0
title_full	<i<CaSPDS</i<, a Spermidine Synthase Gene from Pepper (<i<Capsicum annuum</i< L.), Plays an Important Role in Response to Cold Stress
author_sort	Jianwei Zhang
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author_browse	Jianwei Zhang Minghui Xie Guofeng Yu Dong Wang Zeping Xu Le Liang Jiachang Xiao Yongdong Xie Yi Tang Guochao Sun Bo Sun Zhi Huang Yunsong Lai Huanxiu Li
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title_sort	<i<caspds</i<, a spermidine synthase gene from pepper (<i<capsicum annuum</i< l.), plays an important role in response to cold stress
callnumber	QH301-705.5
title_auth	<i<CaSPDS</i<, a Spermidine Synthase Gene from Pepper (<i<Capsicum annuum</i< L.), Plays an Important Role in Response to Cold Stress
abstract	Spermidine synthase (SPDS) is a key enzyme in the polyamine anabolic pathway. <i<SPDS</i< genes help regulate plant response to environmental stresses, but their roles in pepper remain unclear. In this study, we identified and cloned a SPDS gene from pepper (<i<Capsicum annuum</i< L.), named <i<CaSPDS</i< (LOC107847831). Bioinformatics analysis indicated that <i<CaSPDS</i< contains two highly conserved domains: an SPDS tetramerisation domain and a spermine/SPDS domain. Quantitative reverse-transcription polymerase chain reaction results showed that <i<CaSPDS</i< was highly expressed in the stems, flowers, and mature fruits of pepper and was rapidly induced by cold stress. The function of <i<CaSPDS</i< in cold stress response was studied by silencing and overexpressing it in pepper and <i<Arabidopsis</i<, respectively. Cold injury was more serious and reactive oxygen species levels were greater in the <i<CaSPDS</i<-silenced seedlings than in the wild-type (WT) seedlings after cold treatment. Compared with the WT plants, the <i<CaSPDS</i<-overexpression <i<Arabidopsis</i< plants were more tolerant to cold stress and showed higher antioxidant enzyme activities, spermidine content, and cold-responsive gene (<i<AtCOR15A</i<, <i<AtRD29A</i<, <i<AtCOR47</i<, and <i<AtKIN1</i<) expression. These results indicate that <i<CaSPDS</i< plays important roles in cold stress response and is valuable in molecular breeding to enhance the cold tolerance of pepper.
abstractGer	Spermidine synthase (SPDS) is a key enzyme in the polyamine anabolic pathway. <i<SPDS</i< genes help regulate plant response to environmental stresses, but their roles in pepper remain unclear. In this study, we identified and cloned a SPDS gene from pepper (<i<Capsicum annuum</i< L.), named <i<CaSPDS</i< (LOC107847831). Bioinformatics analysis indicated that <i<CaSPDS</i< contains two highly conserved domains: an SPDS tetramerisation domain and a spermine/SPDS domain. Quantitative reverse-transcription polymerase chain reaction results showed that <i<CaSPDS</i< was highly expressed in the stems, flowers, and mature fruits of pepper and was rapidly induced by cold stress. The function of <i<CaSPDS</i< in cold stress response was studied by silencing and overexpressing it in pepper and <i<Arabidopsis</i<, respectively. Cold injury was more serious and reactive oxygen species levels were greater in the <i<CaSPDS</i<-silenced seedlings than in the wild-type (WT) seedlings after cold treatment. Compared with the WT plants, the <i<CaSPDS</i<-overexpression <i<Arabidopsis</i< plants were more tolerant to cold stress and showed higher antioxidant enzyme activities, spermidine content, and cold-responsive gene (<i<AtCOR15A</i<, <i<AtRD29A</i<, <i<AtCOR47</i<, and <i<AtKIN1</i<) expression. These results indicate that <i<CaSPDS</i< plays important roles in cold stress response and is valuable in molecular breeding to enhance the cold tolerance of pepper.
abstract_unstemmed	Spermidine synthase (SPDS) is a key enzyme in the polyamine anabolic pathway. <i<SPDS</i< genes help regulate plant response to environmental stresses, but their roles in pepper remain unclear. In this study, we identified and cloned a SPDS gene from pepper (<i<Capsicum annuum</i< L.), named <i<CaSPDS</i< (LOC107847831). Bioinformatics analysis indicated that <i<CaSPDS</i< contains two highly conserved domains: an SPDS tetramerisation domain and a spermine/SPDS domain. Quantitative reverse-transcription polymerase chain reaction results showed that <i<CaSPDS</i< was highly expressed in the stems, flowers, and mature fruits of pepper and was rapidly induced by cold stress. The function of <i<CaSPDS</i< in cold stress response was studied by silencing and overexpressing it in pepper and <i<Arabidopsis</i<, respectively. Cold injury was more serious and reactive oxygen species levels were greater in the <i<CaSPDS</i<-silenced seedlings than in the wild-type (WT) seedlings after cold treatment. Compared with the WT plants, the <i<CaSPDS</i<-overexpression <i<Arabidopsis</i< plants were more tolerant to cold stress and showed higher antioxidant enzyme activities, spermidine content, and cold-responsive gene (<i<AtCOR15A</i<, <i<AtRD29A</i<, <i<AtCOR47</i<, and <i<AtKIN1</i<) expression. These results indicate that <i<CaSPDS</i< plays important roles in cold stress response and is valuable in molecular breeding to enhance the cold tolerance of pepper.
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title_short	<i<CaSPDS</i<, a Spermidine Synthase Gene from Pepper (<i<Capsicum annuum</i< L.), Plays an Important Role in Response to Cold Stress
url	https://doi.org/10.3390/ijms24055013 https://doaj.org/article/184b6d28193e41919d72b51cbe49fcd0 https://www.mdpi.com/1422-0067/24/5/5013 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
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<i<CaSPDS</i<, a Spermidine Synthase Gene from Pepper (<i<Capsicum annuum</i< L.), Plays an Important Role in Response to Cold Stress

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