Association of Androgen-Receptor Gene Mutations with the Copy Number of Androgen-Receptor Silk Protein A Complex and Glutathione-S-Transferases T1 and M1 in Prostate Cancer Patients
Objective. The purpose of our work was to explore the association of mutations in the androgen receptor gene and copy numbers of the androgen-receptor silk protein A complex with glutathione-S-transferases T1 and M1 in prostate cancer patients. Materials and Methods. Eighty-five patients with PC and...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Yan Zhang [verfasserIn] Xiangdi Meng [verfasserIn] Zhaosen Ma [verfasserIn] Zhou Sun [verfasserIn] Zhixin Wang [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2023 |
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| Übergeordnetes Werk: |
In: Genetics Research - Hindawi - Cambridge University Press, 2020, (2023) |
|---|---|
| Übergeordnetes Werk: |
year:2023 |
| Links: |
|---|
| DOI / URN: |
10.1155/2023/5956951 |
|---|
| Katalog-ID: |
DOAJ088217000 |
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| 245 | 1 | 0 | |a Association of Androgen-Receptor Gene Mutations with the Copy Number of Androgen-Receptor Silk Protein A Complex and Glutathione-S-Transferases T1 and M1 in Prostate Cancer Patients |
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| 520 | |a Objective. The purpose of our work was to explore the association of mutations in the androgen receptor gene and copy numbers of the androgen-receptor silk protein A complex with glutathione-S-transferases T1 and M1 in prostate cancer patients. Materials and Methods. Eighty-five patients with PC and 85 healthy controls were included in the study. Fasting peripheral venous blood was collected, whole blood genomic DNA was extracted, and AR gene-receptor genotype was detected by a high-resolution melting curve analysis detection technology. Expression levels of androgen receptor (AR) and filamin protein A (FlnA) were detected by Western blotting. RT-PCR was used to detect the copy number of T1 and M1 glutathione-S-transferases. Results. The wild-type androgen receptor gene rs5918762 is of TT type. The frequencies of CC and TC genes in the prostate cancer group were significantly higher than those in the normal control group P<0.05. Compared with TT-type PC patients, PC patients with TC-type and CC-type had higher expression levels of sex hormone receptor silk protein A complex and higher copy numbers of GSTT1 and GSTM1 P<0.05. Androgen-receptor gene mutation (T ⟶ C) was significantly positively correlated with the expression level of androgen-receptor silk protein A complex and the copy number of GSTT1 and GSTM1. Conclusion. Androgen-receptor gene polymorphisms were significantly associated with expression levels of androgen receptor complex A and silk proteins, and copy numbers of T1 and M1 glutathione-S-transferases. A combination of four factors can be used to identify prostate cancer susceptibility and disease progression. | ||
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10.1155/2023/5956951 doi (DE-627)DOAJ088217000 (DE-599)DOAJccfaecde549948d788fdaa32a6e07cc9 DE-627 ger DE-627 rakwb eng QH426-470 Yan Zhang verfasserin aut Association of Androgen-Receptor Gene Mutations with the Copy Number of Androgen-Receptor Silk Protein A Complex and Glutathione-S-Transferases T1 and M1 in Prostate Cancer Patients 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective. The purpose of our work was to explore the association of mutations in the androgen receptor gene and copy numbers of the androgen-receptor silk protein A complex with glutathione-S-transferases T1 and M1 in prostate cancer patients. Materials and Methods. Eighty-five patients with PC and 85 healthy controls were included in the study. Fasting peripheral venous blood was collected, whole blood genomic DNA was extracted, and AR gene-receptor genotype was detected by a high-resolution melting curve analysis detection technology. Expression levels of androgen receptor (AR) and filamin protein A (FlnA) were detected by Western blotting. RT-PCR was used to detect the copy number of T1 and M1 glutathione-S-transferases. Results. The wild-type androgen receptor gene rs5918762 is of TT type. The frequencies of CC and TC genes in the prostate cancer group were significantly higher than those in the normal control group P<0.05. Compared with TT-type PC patients, PC patients with TC-type and CC-type had higher expression levels of sex hormone receptor silk protein A complex and higher copy numbers of GSTT1 and GSTM1 P<0.05. Androgen-receptor gene mutation (T ⟶ C) was significantly positively correlated with the expression level of androgen-receptor silk protein A complex and the copy number of GSTT1 and GSTM1. Conclusion. Androgen-receptor gene polymorphisms were significantly associated with expression levels of androgen receptor complex A and silk proteins, and copy numbers of T1 and M1 glutathione-S-transferases. A combination of four factors can be used to identify prostate cancer susceptibility and disease progression. Genetics Xiangdi Meng verfasserin aut Zhaosen Ma verfasserin aut Zhou Sun verfasserin aut Zhixin Wang verfasserin aut In Genetics Research Hindawi - Cambridge University Press, 2020 (2023) (DE-627)26813233X (DE-600)1472156-9 14695073 nnns year:2023 https://doi.org/10.1155/2023/5956951 kostenfrei https://doaj.org/article/ccfaecde549948d788fdaa32a6e07cc9 kostenfrei http://dx.doi.org/10.1155/2023/5956951 kostenfrei https://doaj.org/toc/1469-5073 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 AR 2023 |
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10.1155/2023/5956951 doi (DE-627)DOAJ088217000 (DE-599)DOAJccfaecde549948d788fdaa32a6e07cc9 DE-627 ger DE-627 rakwb eng QH426-470 Yan Zhang verfasserin aut Association of Androgen-Receptor Gene Mutations with the Copy Number of Androgen-Receptor Silk Protein A Complex and Glutathione-S-Transferases T1 and M1 in Prostate Cancer Patients 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective. The purpose of our work was to explore the association of mutations in the androgen receptor gene and copy numbers of the androgen-receptor silk protein A complex with glutathione-S-transferases T1 and M1 in prostate cancer patients. Materials and Methods. Eighty-five patients with PC and 85 healthy controls were included in the study. Fasting peripheral venous blood was collected, whole blood genomic DNA was extracted, and AR gene-receptor genotype was detected by a high-resolution melting curve analysis detection technology. Expression levels of androgen receptor (AR) and filamin protein A (FlnA) were detected by Western blotting. RT-PCR was used to detect the copy number of T1 and M1 glutathione-S-transferases. Results. The wild-type androgen receptor gene rs5918762 is of TT type. The frequencies of CC and TC genes in the prostate cancer group were significantly higher than those in the normal control group P<0.05. Compared with TT-type PC patients, PC patients with TC-type and CC-type had higher expression levels of sex hormone receptor silk protein A complex and higher copy numbers of GSTT1 and GSTM1 P<0.05. Androgen-receptor gene mutation (T ⟶ C) was significantly positively correlated with the expression level of androgen-receptor silk protein A complex and the copy number of GSTT1 and GSTM1. Conclusion. Androgen-receptor gene polymorphisms were significantly associated with expression levels of androgen receptor complex A and silk proteins, and copy numbers of T1 and M1 glutathione-S-transferases. A combination of four factors can be used to identify prostate cancer susceptibility and disease progression. Genetics Xiangdi Meng verfasserin aut Zhaosen Ma verfasserin aut Zhou Sun verfasserin aut Zhixin Wang verfasserin aut In Genetics Research Hindawi - Cambridge University Press, 2020 (2023) (DE-627)26813233X (DE-600)1472156-9 14695073 nnns year:2023 https://doi.org/10.1155/2023/5956951 kostenfrei https://doaj.org/article/ccfaecde549948d788fdaa32a6e07cc9 kostenfrei http://dx.doi.org/10.1155/2023/5956951 kostenfrei https://doaj.org/toc/1469-5073 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 AR 2023 |
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10.1155/2023/5956951 doi (DE-627)DOAJ088217000 (DE-599)DOAJccfaecde549948d788fdaa32a6e07cc9 DE-627 ger DE-627 rakwb eng QH426-470 Yan Zhang verfasserin aut Association of Androgen-Receptor Gene Mutations with the Copy Number of Androgen-Receptor Silk Protein A Complex and Glutathione-S-Transferases T1 and M1 in Prostate Cancer Patients 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective. The purpose of our work was to explore the association of mutations in the androgen receptor gene and copy numbers of the androgen-receptor silk protein A complex with glutathione-S-transferases T1 and M1 in prostate cancer patients. Materials and Methods. Eighty-five patients with PC and 85 healthy controls were included in the study. Fasting peripheral venous blood was collected, whole blood genomic DNA was extracted, and AR gene-receptor genotype was detected by a high-resolution melting curve analysis detection technology. Expression levels of androgen receptor (AR) and filamin protein A (FlnA) were detected by Western blotting. RT-PCR was used to detect the copy number of T1 and M1 glutathione-S-transferases. Results. The wild-type androgen receptor gene rs5918762 is of TT type. The frequencies of CC and TC genes in the prostate cancer group were significantly higher than those in the normal control group P<0.05. Compared with TT-type PC patients, PC patients with TC-type and CC-type had higher expression levels of sex hormone receptor silk protein A complex and higher copy numbers of GSTT1 and GSTM1 P<0.05. Androgen-receptor gene mutation (T ⟶ C) was significantly positively correlated with the expression level of androgen-receptor silk protein A complex and the copy number of GSTT1 and GSTM1. Conclusion. Androgen-receptor gene polymorphisms were significantly associated with expression levels of androgen receptor complex A and silk proteins, and copy numbers of T1 and M1 glutathione-S-transferases. A combination of four factors can be used to identify prostate cancer susceptibility and disease progression. Genetics Xiangdi Meng verfasserin aut Zhaosen Ma verfasserin aut Zhou Sun verfasserin aut Zhixin Wang verfasserin aut In Genetics Research Hindawi - Cambridge University Press, 2020 (2023) (DE-627)26813233X (DE-600)1472156-9 14695073 nnns year:2023 https://doi.org/10.1155/2023/5956951 kostenfrei https://doaj.org/article/ccfaecde549948d788fdaa32a6e07cc9 kostenfrei http://dx.doi.org/10.1155/2023/5956951 kostenfrei https://doaj.org/toc/1469-5073 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 AR 2023 |
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10.1155/2023/5956951 doi (DE-627)DOAJ088217000 (DE-599)DOAJccfaecde549948d788fdaa32a6e07cc9 DE-627 ger DE-627 rakwb eng QH426-470 Yan Zhang verfasserin aut Association of Androgen-Receptor Gene Mutations with the Copy Number of Androgen-Receptor Silk Protein A Complex and Glutathione-S-Transferases T1 and M1 in Prostate Cancer Patients 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective. The purpose of our work was to explore the association of mutations in the androgen receptor gene and copy numbers of the androgen-receptor silk protein A complex with glutathione-S-transferases T1 and M1 in prostate cancer patients. Materials and Methods. Eighty-five patients with PC and 85 healthy controls were included in the study. Fasting peripheral venous blood was collected, whole blood genomic DNA was extracted, and AR gene-receptor genotype was detected by a high-resolution melting curve analysis detection technology. Expression levels of androgen receptor (AR) and filamin protein A (FlnA) were detected by Western blotting. RT-PCR was used to detect the copy number of T1 and M1 glutathione-S-transferases. Results. The wild-type androgen receptor gene rs5918762 is of TT type. The frequencies of CC and TC genes in the prostate cancer group were significantly higher than those in the normal control group P<0.05. Compared with TT-type PC patients, PC patients with TC-type and CC-type had higher expression levels of sex hormone receptor silk protein A complex and higher copy numbers of GSTT1 and GSTM1 P<0.05. Androgen-receptor gene mutation (T ⟶ C) was significantly positively correlated with the expression level of androgen-receptor silk protein A complex and the copy number of GSTT1 and GSTM1. Conclusion. Androgen-receptor gene polymorphisms were significantly associated with expression levels of androgen receptor complex A and silk proteins, and copy numbers of T1 and M1 glutathione-S-transferases. A combination of four factors can be used to identify prostate cancer susceptibility and disease progression. Genetics Xiangdi Meng verfasserin aut Zhaosen Ma verfasserin aut Zhou Sun verfasserin aut Zhixin Wang verfasserin aut In Genetics Research Hindawi - Cambridge University Press, 2020 (2023) (DE-627)26813233X (DE-600)1472156-9 14695073 nnns year:2023 https://doi.org/10.1155/2023/5956951 kostenfrei https://doaj.org/article/ccfaecde549948d788fdaa32a6e07cc9 kostenfrei http://dx.doi.org/10.1155/2023/5956951 kostenfrei https://doaj.org/toc/1469-5073 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 AR 2023 |
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10.1155/2023/5956951 doi (DE-627)DOAJ088217000 (DE-599)DOAJccfaecde549948d788fdaa32a6e07cc9 DE-627 ger DE-627 rakwb eng QH426-470 Yan Zhang verfasserin aut Association of Androgen-Receptor Gene Mutations with the Copy Number of Androgen-Receptor Silk Protein A Complex and Glutathione-S-Transferases T1 and M1 in Prostate Cancer Patients 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective. The purpose of our work was to explore the association of mutations in the androgen receptor gene and copy numbers of the androgen-receptor silk protein A complex with glutathione-S-transferases T1 and M1 in prostate cancer patients. Materials and Methods. Eighty-five patients with PC and 85 healthy controls were included in the study. Fasting peripheral venous blood was collected, whole blood genomic DNA was extracted, and AR gene-receptor genotype was detected by a high-resolution melting curve analysis detection technology. Expression levels of androgen receptor (AR) and filamin protein A (FlnA) were detected by Western blotting. RT-PCR was used to detect the copy number of T1 and M1 glutathione-S-transferases. Results. The wild-type androgen receptor gene rs5918762 is of TT type. The frequencies of CC and TC genes in the prostate cancer group were significantly higher than those in the normal control group P<0.05. Compared with TT-type PC patients, PC patients with TC-type and CC-type had higher expression levels of sex hormone receptor silk protein A complex and higher copy numbers of GSTT1 and GSTM1 P<0.05. Androgen-receptor gene mutation (T ⟶ C) was significantly positively correlated with the expression level of androgen-receptor silk protein A complex and the copy number of GSTT1 and GSTM1. Conclusion. Androgen-receptor gene polymorphisms were significantly associated with expression levels of androgen receptor complex A and silk proteins, and copy numbers of T1 and M1 glutathione-S-transferases. A combination of four factors can be used to identify prostate cancer susceptibility and disease progression. Genetics Xiangdi Meng verfasserin aut Zhaosen Ma verfasserin aut Zhou Sun verfasserin aut Zhixin Wang verfasserin aut In Genetics Research Hindawi - Cambridge University Press, 2020 (2023) (DE-627)26813233X (DE-600)1472156-9 14695073 nnns year:2023 https://doi.org/10.1155/2023/5956951 kostenfrei https://doaj.org/article/ccfaecde549948d788fdaa32a6e07cc9 kostenfrei http://dx.doi.org/10.1155/2023/5956951 kostenfrei https://doaj.org/toc/1469-5073 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 AR 2023 |
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Association of Androgen-Receptor Gene Mutations with the Copy Number of Androgen-Receptor Silk Protein A Complex and Glutathione-S-Transferases T1 and M1 in Prostate Cancer Patients |
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Association of Androgen-Receptor Gene Mutations with the Copy Number of Androgen-Receptor Silk Protein A Complex and Glutathione-S-Transferases T1 and M1 in Prostate Cancer Patients |
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Yan Zhang |
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Genetics Research |
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Genetics Research |
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Yan Zhang Xiangdi Meng Zhaosen Ma Zhou Sun Zhixin Wang |
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Elektronische Aufsätze |
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Yan Zhang |
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10.1155/2023/5956951 |
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verfasserin |
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association of androgen-receptor gene mutations with the copy number of androgen-receptor silk protein a complex and glutathione-s-transferases t1 and m1 in prostate cancer patients |
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QH426-470 |
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Association of Androgen-Receptor Gene Mutations with the Copy Number of Androgen-Receptor Silk Protein A Complex and Glutathione-S-Transferases T1 and M1 in Prostate Cancer Patients |
| abstract |
Objective. The purpose of our work was to explore the association of mutations in the androgen receptor gene and copy numbers of the androgen-receptor silk protein A complex with glutathione-S-transferases T1 and M1 in prostate cancer patients. Materials and Methods. Eighty-five patients with PC and 85 healthy controls were included in the study. Fasting peripheral venous blood was collected, whole blood genomic DNA was extracted, and AR gene-receptor genotype was detected by a high-resolution melting curve analysis detection technology. Expression levels of androgen receptor (AR) and filamin protein A (FlnA) were detected by Western blotting. RT-PCR was used to detect the copy number of T1 and M1 glutathione-S-transferases. Results. The wild-type androgen receptor gene rs5918762 is of TT type. The frequencies of CC and TC genes in the prostate cancer group were significantly higher than those in the normal control group P<0.05. Compared with TT-type PC patients, PC patients with TC-type and CC-type had higher expression levels of sex hormone receptor silk protein A complex and higher copy numbers of GSTT1 and GSTM1 P<0.05. Androgen-receptor gene mutation (T ⟶ C) was significantly positively correlated with the expression level of androgen-receptor silk protein A complex and the copy number of GSTT1 and GSTM1. Conclusion. Androgen-receptor gene polymorphisms were significantly associated with expression levels of androgen receptor complex A and silk proteins, and copy numbers of T1 and M1 glutathione-S-transferases. A combination of four factors can be used to identify prostate cancer susceptibility and disease progression. |
| abstractGer |
Objective. The purpose of our work was to explore the association of mutations in the androgen receptor gene and copy numbers of the androgen-receptor silk protein A complex with glutathione-S-transferases T1 and M1 in prostate cancer patients. Materials and Methods. Eighty-five patients with PC and 85 healthy controls were included in the study. Fasting peripheral venous blood was collected, whole blood genomic DNA was extracted, and AR gene-receptor genotype was detected by a high-resolution melting curve analysis detection technology. Expression levels of androgen receptor (AR) and filamin protein A (FlnA) were detected by Western blotting. RT-PCR was used to detect the copy number of T1 and M1 glutathione-S-transferases. Results. The wild-type androgen receptor gene rs5918762 is of TT type. The frequencies of CC and TC genes in the prostate cancer group were significantly higher than those in the normal control group P<0.05. Compared with TT-type PC patients, PC patients with TC-type and CC-type had higher expression levels of sex hormone receptor silk protein A complex and higher copy numbers of GSTT1 and GSTM1 P<0.05. Androgen-receptor gene mutation (T ⟶ C) was significantly positively correlated with the expression level of androgen-receptor silk protein A complex and the copy number of GSTT1 and GSTM1. Conclusion. Androgen-receptor gene polymorphisms were significantly associated with expression levels of androgen receptor complex A and silk proteins, and copy numbers of T1 and M1 glutathione-S-transferases. A combination of four factors can be used to identify prostate cancer susceptibility and disease progression. |
| abstract_unstemmed |
Objective. The purpose of our work was to explore the association of mutations in the androgen receptor gene and copy numbers of the androgen-receptor silk protein A complex with glutathione-S-transferases T1 and M1 in prostate cancer patients. Materials and Methods. Eighty-five patients with PC and 85 healthy controls were included in the study. Fasting peripheral venous blood was collected, whole blood genomic DNA was extracted, and AR gene-receptor genotype was detected by a high-resolution melting curve analysis detection technology. Expression levels of androgen receptor (AR) and filamin protein A (FlnA) were detected by Western blotting. RT-PCR was used to detect the copy number of T1 and M1 glutathione-S-transferases. Results. The wild-type androgen receptor gene rs5918762 is of TT type. The frequencies of CC and TC genes in the prostate cancer group were significantly higher than those in the normal control group P<0.05. Compared with TT-type PC patients, PC patients with TC-type and CC-type had higher expression levels of sex hormone receptor silk protein A complex and higher copy numbers of GSTT1 and GSTM1 P<0.05. Androgen-receptor gene mutation (T ⟶ C) was significantly positively correlated with the expression level of androgen-receptor silk protein A complex and the copy number of GSTT1 and GSTM1. Conclusion. Androgen-receptor gene polymorphisms were significantly associated with expression levels of androgen receptor complex A and silk proteins, and copy numbers of T1 and M1 glutathione-S-transferases. A combination of four factors can be used to identify prostate cancer susceptibility and disease progression. |
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Association of Androgen-Receptor Gene Mutations with the Copy Number of Androgen-Receptor Silk Protein A Complex and Glutathione-S-Transferases T1 and M1 in Prostate Cancer Patients |
| url |
https://doi.org/10.1155/2023/5956951 https://doaj.org/article/ccfaecde549948d788fdaa32a6e07cc9 http://dx.doi.org/10.1155/2023/5956951 https://doaj.org/toc/1469-5073 |
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Xiangdi Meng Zhaosen Ma Zhou Sun Zhixin Wang |
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