Platelet pannexin-1 channels modulate neutrophil activation and migration but not the progression of abdominal aortic aneurysm

Abdominal aortic aneurysm (AAA) is a common disease and highly lethal if untreated. The progressive dilatation of the abdominal aorta is accompanied by degradation and remodeling of the vessel wall due to chronic inflammation. Pannexins represent anion-selective channels and play a crucial role in non-vesicular ATP release to amplify paracrine signaling in cells. Thus, pannexins are involved in many (patho-) physiological processes. Recently, Panx1 channels were identified to be significantly involved in abdominal aortic aneurysm formation through endothelial derived Panx1 regulated inflammation and aortic remodeling. In platelets, Panx1 becomes activated following activation of glycoprotein (GP) VI. Since platelets play a role in cardiovascular diseases including abdominal aortic aneurysm, we analyzed the contribution of platelet Panx1 in the progression of abdominal aortic aneurysm. We detected enhanced Panx1 plasma levels in abdominal aortic aneurysm patients. In experimental abdominal aortic aneurysm using the pancreatic porcine elastase (PPE) mouse model, a major contribution of platelet Panx1 channels in platelet activation, pro-coagulant activity of platelets and platelet-mediated inflammation has been detected. In detail, platelets are important for the migration of neutrophils into the aortic wall induced by direct cell interaction and by activation of endothelial cells. Decreased platelet activation and inflammation did not affect ECM remodeling or wall thickness in platelet-specific Panx1 knock-out mice following PPE surgery. Thus, aortic diameter expansion at different time points after elastase infusion of the aortic wall was unaltered in platelet-specific Panx1 deficient mice suggesting that the modulation of inflammation alone does not affect abdominal aortic aneurysm formation and progression. In conclusion, our data strongly supports the role of platelets in inflammatory responses in abdominal aortic aneurysm via Panx1 channels and adds important knowledge about the significance of platelets in abdominal aortic aneurysm pathology important for the establishment of an anti-platelet therapy for abdominal aortic aneurysm patients. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Lisa Maria Metz [verfasserIn] Tobias Feige [verfasserIn] Larissa de Biasi [verfasserIn] Agnes Ehrenberg [verfasserIn] Joscha Mulorz [verfasserIn] Laura Mara Toska [verfasserIn] Friedrich Reusswig [verfasserIn] Christine Quast [verfasserIn] Norbert Gerdes [verfasserIn] Malte Kelm [verfasserIn] Hubert Schelzig [verfasserIn] Margitta Elvers [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	platelets pannexin-1 abdominal aortic aneurysm inflammation ECM remodeling selectin

Übergeordnetes Werk:	In: Frontiers in Molecular Biosciences - Frontiers Media S.A., 2015, 10(2023)
Übergeordnetes Werk:	volume:10 ; year:2023

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fmolb.2023.1111108

Katalog-ID:	DOAJ08826985X

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allfields	10.3389/fmolb.2023.1111108 doi (DE-627)DOAJ08826985X (DE-599)DOAJ715ce80f15904c5caf775f925230aee4 DE-627 ger DE-627 rakwb eng QH301-705.5 Lisa Maria Metz verfasserin aut Platelet pannexin-1 channels modulate neutrophil activation and migration but not the progression of abdominal aortic aneurysm 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abdominal aortic aneurysm (AAA) is a common disease and highly lethal if untreated. The progressive dilatation of the abdominal aorta is accompanied by degradation and remodeling of the vessel wall due to chronic inflammation. Pannexins represent anion-selective channels and play a crucial role in non-vesicular ATP release to amplify paracrine signaling in cells. Thus, pannexins are involved in many (patho-) physiological processes. Recently, Panx1 channels were identified to be significantly involved in abdominal aortic aneurysm formation through endothelial derived Panx1 regulated inflammation and aortic remodeling. In platelets, Panx1 becomes activated following activation of glycoprotein (GP) VI. Since platelets play a role in cardiovascular diseases including abdominal aortic aneurysm, we analyzed the contribution of platelet Panx1 in the progression of abdominal aortic aneurysm. We detected enhanced Panx1 plasma levels in abdominal aortic aneurysm patients. In experimental abdominal aortic aneurysm using the pancreatic porcine elastase (PPE) mouse model, a major contribution of platelet Panx1 channels in platelet activation, pro-coagulant activity of platelets and platelet-mediated inflammation has been detected. In detail, platelets are important for the migration of neutrophils into the aortic wall induced by direct cell interaction and by activation of endothelial cells. Decreased platelet activation and inflammation did not affect ECM remodeling or wall thickness in platelet-specific Panx1 knock-out mice following PPE surgery. Thus, aortic diameter expansion at different time points after elastase infusion of the aortic wall was unaltered in platelet-specific Panx1 deficient mice suggesting that the modulation of inflammation alone does not affect abdominal aortic aneurysm formation and progression. In conclusion, our data strongly supports the role of platelets in inflammatory responses in abdominal aortic aneurysm via Panx1 channels and adds important knowledge about the significance of platelets in abdominal aortic aneurysm pathology important for the establishment of an anti-platelet therapy for abdominal aortic aneurysm patients. platelets pannexin-1 abdominal aortic aneurysm inflammation ECM remodeling selectin Biology (General) Tobias Feige verfasserin aut Larissa de Biasi verfasserin aut Agnes Ehrenberg verfasserin aut Joscha Mulorz verfasserin aut Laura Mara Toska verfasserin aut Friedrich Reusswig verfasserin aut Christine Quast verfasserin aut Norbert Gerdes verfasserin aut Malte Kelm verfasserin aut Hubert Schelzig verfasserin aut Margitta Elvers verfasserin aut In Frontiers in Molecular Biosciences Frontiers Media S.A., 2015 10(2023) (DE-627)820039691 (DE-600)2814330-9 2296889X nnns volume:10 year:2023 https://doi.org/10.3389/fmolb.2023.1111108 kostenfrei https://doaj.org/article/715ce80f15904c5caf775f925230aee4 kostenfrei https://www.frontiersin.org/articles/10.3389/fmolb.2023.1111108/full kostenfrei https://doaj.org/toc/2296-889X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2023
spelling	10.3389/fmolb.2023.1111108 doi (DE-627)DOAJ08826985X (DE-599)DOAJ715ce80f15904c5caf775f925230aee4 DE-627 ger DE-627 rakwb eng QH301-705.5 Lisa Maria Metz verfasserin aut Platelet pannexin-1 channels modulate neutrophil activation and migration but not the progression of abdominal aortic aneurysm 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abdominal aortic aneurysm (AAA) is a common disease and highly lethal if untreated. The progressive dilatation of the abdominal aorta is accompanied by degradation and remodeling of the vessel wall due to chronic inflammation. Pannexins represent anion-selective channels and play a crucial role in non-vesicular ATP release to amplify paracrine signaling in cells. Thus, pannexins are involved in many (patho-) physiological processes. Recently, Panx1 channels were identified to be significantly involved in abdominal aortic aneurysm formation through endothelial derived Panx1 regulated inflammation and aortic remodeling. In platelets, Panx1 becomes activated following activation of glycoprotein (GP) VI. Since platelets play a role in cardiovascular diseases including abdominal aortic aneurysm, we analyzed the contribution of platelet Panx1 in the progression of abdominal aortic aneurysm. We detected enhanced Panx1 plasma levels in abdominal aortic aneurysm patients. In experimental abdominal aortic aneurysm using the pancreatic porcine elastase (PPE) mouse model, a major contribution of platelet Panx1 channels in platelet activation, pro-coagulant activity of platelets and platelet-mediated inflammation has been detected. In detail, platelets are important for the migration of neutrophils into the aortic wall induced by direct cell interaction and by activation of endothelial cells. Decreased platelet activation and inflammation did not affect ECM remodeling or wall thickness in platelet-specific Panx1 knock-out mice following PPE surgery. Thus, aortic diameter expansion at different time points after elastase infusion of the aortic wall was unaltered in platelet-specific Panx1 deficient mice suggesting that the modulation of inflammation alone does not affect abdominal aortic aneurysm formation and progression. In conclusion, our data strongly supports the role of platelets in inflammatory responses in abdominal aortic aneurysm via Panx1 channels and adds important knowledge about the significance of platelets in abdominal aortic aneurysm pathology important for the establishment of an anti-platelet therapy for abdominal aortic aneurysm patients. platelets pannexin-1 abdominal aortic aneurysm inflammation ECM remodeling selectin Biology (General) Tobias Feige verfasserin aut Larissa de Biasi verfasserin aut Agnes Ehrenberg verfasserin aut Joscha Mulorz verfasserin aut Laura Mara Toska verfasserin aut Friedrich Reusswig verfasserin aut Christine Quast verfasserin aut Norbert Gerdes verfasserin aut Malte Kelm verfasserin aut Hubert Schelzig verfasserin aut Margitta Elvers verfasserin aut In Frontiers in Molecular Biosciences Frontiers Media S.A., 2015 10(2023) (DE-627)820039691 (DE-600)2814330-9 2296889X nnns volume:10 year:2023 https://doi.org/10.3389/fmolb.2023.1111108 kostenfrei https://doaj.org/article/715ce80f15904c5caf775f925230aee4 kostenfrei https://www.frontiersin.org/articles/10.3389/fmolb.2023.1111108/full kostenfrei https://doaj.org/toc/2296-889X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2023
allfields_unstemmed	10.3389/fmolb.2023.1111108 doi (DE-627)DOAJ08826985X (DE-599)DOAJ715ce80f15904c5caf775f925230aee4 DE-627 ger DE-627 rakwb eng QH301-705.5 Lisa Maria Metz verfasserin aut Platelet pannexin-1 channels modulate neutrophil activation and migration but not the progression of abdominal aortic aneurysm 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abdominal aortic aneurysm (AAA) is a common disease and highly lethal if untreated. The progressive dilatation of the abdominal aorta is accompanied by degradation and remodeling of the vessel wall due to chronic inflammation. Pannexins represent anion-selective channels and play a crucial role in non-vesicular ATP release to amplify paracrine signaling in cells. Thus, pannexins are involved in many (patho-) physiological processes. Recently, Panx1 channels were identified to be significantly involved in abdominal aortic aneurysm formation through endothelial derived Panx1 regulated inflammation and aortic remodeling. In platelets, Panx1 becomes activated following activation of glycoprotein (GP) VI. Since platelets play a role in cardiovascular diseases including abdominal aortic aneurysm, we analyzed the contribution of platelet Panx1 in the progression of abdominal aortic aneurysm. We detected enhanced Panx1 plasma levels in abdominal aortic aneurysm patients. In experimental abdominal aortic aneurysm using the pancreatic porcine elastase (PPE) mouse model, a major contribution of platelet Panx1 channels in platelet activation, pro-coagulant activity of platelets and platelet-mediated inflammation has been detected. In detail, platelets are important for the migration of neutrophils into the aortic wall induced by direct cell interaction and by activation of endothelial cells. Decreased platelet activation and inflammation did not affect ECM remodeling or wall thickness in platelet-specific Panx1 knock-out mice following PPE surgery. Thus, aortic diameter expansion at different time points after elastase infusion of the aortic wall was unaltered in platelet-specific Panx1 deficient mice suggesting that the modulation of inflammation alone does not affect abdominal aortic aneurysm formation and progression. In conclusion, our data strongly supports the role of platelets in inflammatory responses in abdominal aortic aneurysm via Panx1 channels and adds important knowledge about the significance of platelets in abdominal aortic aneurysm pathology important for the establishment of an anti-platelet therapy for abdominal aortic aneurysm patients. platelets pannexin-1 abdominal aortic aneurysm inflammation ECM remodeling selectin Biology (General) Tobias Feige verfasserin aut Larissa de Biasi verfasserin aut Agnes Ehrenberg verfasserin aut Joscha Mulorz verfasserin aut Laura Mara Toska verfasserin aut Friedrich Reusswig verfasserin aut Christine Quast verfasserin aut Norbert Gerdes verfasserin aut Malte Kelm verfasserin aut Hubert Schelzig verfasserin aut Margitta Elvers verfasserin aut In Frontiers in Molecular Biosciences Frontiers Media S.A., 2015 10(2023) (DE-627)820039691 (DE-600)2814330-9 2296889X nnns volume:10 year:2023 https://doi.org/10.3389/fmolb.2023.1111108 kostenfrei https://doaj.org/article/715ce80f15904c5caf775f925230aee4 kostenfrei https://www.frontiersin.org/articles/10.3389/fmolb.2023.1111108/full kostenfrei https://doaj.org/toc/2296-889X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2023
allfieldsGer	10.3389/fmolb.2023.1111108 doi (DE-627)DOAJ08826985X (DE-599)DOAJ715ce80f15904c5caf775f925230aee4 DE-627 ger DE-627 rakwb eng QH301-705.5 Lisa Maria Metz verfasserin aut Platelet pannexin-1 channels modulate neutrophil activation and migration but not the progression of abdominal aortic aneurysm 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abdominal aortic aneurysm (AAA) is a common disease and highly lethal if untreated. The progressive dilatation of the abdominal aorta is accompanied by degradation and remodeling of the vessel wall due to chronic inflammation. Pannexins represent anion-selective channels and play a crucial role in non-vesicular ATP release to amplify paracrine signaling in cells. Thus, pannexins are involved in many (patho-) physiological processes. Recently, Panx1 channels were identified to be significantly involved in abdominal aortic aneurysm formation through endothelial derived Panx1 regulated inflammation and aortic remodeling. In platelets, Panx1 becomes activated following activation of glycoprotein (GP) VI. Since platelets play a role in cardiovascular diseases including abdominal aortic aneurysm, we analyzed the contribution of platelet Panx1 in the progression of abdominal aortic aneurysm. We detected enhanced Panx1 plasma levels in abdominal aortic aneurysm patients. In experimental abdominal aortic aneurysm using the pancreatic porcine elastase (PPE) mouse model, a major contribution of platelet Panx1 channels in platelet activation, pro-coagulant activity of platelets and platelet-mediated inflammation has been detected. In detail, platelets are important for the migration of neutrophils into the aortic wall induced by direct cell interaction and by activation of endothelial cells. Decreased platelet activation and inflammation did not affect ECM remodeling or wall thickness in platelet-specific Panx1 knock-out mice following PPE surgery. Thus, aortic diameter expansion at different time points after elastase infusion of the aortic wall was unaltered in platelet-specific Panx1 deficient mice suggesting that the modulation of inflammation alone does not affect abdominal aortic aneurysm formation and progression. In conclusion, our data strongly supports the role of platelets in inflammatory responses in abdominal aortic aneurysm via Panx1 channels and adds important knowledge about the significance of platelets in abdominal aortic aneurysm pathology important for the establishment of an anti-platelet therapy for abdominal aortic aneurysm patients. platelets pannexin-1 abdominal aortic aneurysm inflammation ECM remodeling selectin Biology (General) Tobias Feige verfasserin aut Larissa de Biasi verfasserin aut Agnes Ehrenberg verfasserin aut Joscha Mulorz verfasserin aut Laura Mara Toska verfasserin aut Friedrich Reusswig verfasserin aut Christine Quast verfasserin aut Norbert Gerdes verfasserin aut Malte Kelm verfasserin aut Hubert Schelzig verfasserin aut Margitta Elvers verfasserin aut In Frontiers in Molecular Biosciences Frontiers Media S.A., 2015 10(2023) (DE-627)820039691 (DE-600)2814330-9 2296889X nnns volume:10 year:2023 https://doi.org/10.3389/fmolb.2023.1111108 kostenfrei https://doaj.org/article/715ce80f15904c5caf775f925230aee4 kostenfrei https://www.frontiersin.org/articles/10.3389/fmolb.2023.1111108/full kostenfrei https://doaj.org/toc/2296-889X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2023
allfieldsSound	10.3389/fmolb.2023.1111108 doi (DE-627)DOAJ08826985X (DE-599)DOAJ715ce80f15904c5caf775f925230aee4 DE-627 ger DE-627 rakwb eng QH301-705.5 Lisa Maria Metz verfasserin aut Platelet pannexin-1 channels modulate neutrophil activation and migration but not the progression of abdominal aortic aneurysm 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abdominal aortic aneurysm (AAA) is a common disease and highly lethal if untreated. The progressive dilatation of the abdominal aorta is accompanied by degradation and remodeling of the vessel wall due to chronic inflammation. Pannexins represent anion-selective channels and play a crucial role in non-vesicular ATP release to amplify paracrine signaling in cells. Thus, pannexins are involved in many (patho-) physiological processes. Recently, Panx1 channels were identified to be significantly involved in abdominal aortic aneurysm formation through endothelial derived Panx1 regulated inflammation and aortic remodeling. In platelets, Panx1 becomes activated following activation of glycoprotein (GP) VI. Since platelets play a role in cardiovascular diseases including abdominal aortic aneurysm, we analyzed the contribution of platelet Panx1 in the progression of abdominal aortic aneurysm. We detected enhanced Panx1 plasma levels in abdominal aortic aneurysm patients. In experimental abdominal aortic aneurysm using the pancreatic porcine elastase (PPE) mouse model, a major contribution of platelet Panx1 channels in platelet activation, pro-coagulant activity of platelets and platelet-mediated inflammation has been detected. In detail, platelets are important for the migration of neutrophils into the aortic wall induced by direct cell interaction and by activation of endothelial cells. Decreased platelet activation and inflammation did not affect ECM remodeling or wall thickness in platelet-specific Panx1 knock-out mice following PPE surgery. Thus, aortic diameter expansion at different time points after elastase infusion of the aortic wall was unaltered in platelet-specific Panx1 deficient mice suggesting that the modulation of inflammation alone does not affect abdominal aortic aneurysm formation and progression. In conclusion, our data strongly supports the role of platelets in inflammatory responses in abdominal aortic aneurysm via Panx1 channels and adds important knowledge about the significance of platelets in abdominal aortic aneurysm pathology important for the establishment of an anti-platelet therapy for abdominal aortic aneurysm patients. platelets pannexin-1 abdominal aortic aneurysm inflammation ECM remodeling selectin Biology (General) Tobias Feige verfasserin aut Larissa de Biasi verfasserin aut Agnes Ehrenberg verfasserin aut Joscha Mulorz verfasserin aut Laura Mara Toska verfasserin aut Friedrich Reusswig verfasserin aut Christine Quast verfasserin aut Norbert Gerdes verfasserin aut Malte Kelm verfasserin aut Hubert Schelzig verfasserin aut Margitta Elvers verfasserin aut In Frontiers in Molecular Biosciences Frontiers Media S.A., 2015 10(2023) (DE-627)820039691 (DE-600)2814330-9 2296889X nnns volume:10 year:2023 https://doi.org/10.3389/fmolb.2023.1111108 kostenfrei https://doaj.org/article/715ce80f15904c5caf775f925230aee4 kostenfrei https://www.frontiersin.org/articles/10.3389/fmolb.2023.1111108/full kostenfrei https://doaj.org/toc/2296-889X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2023
language	English
source	In Frontiers in Molecular Biosciences 10(2023) volume:10 year:2023
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topic_facet	platelets pannexin-1 abdominal aortic aneurysm inflammation ECM remodeling selectin Biology (General)
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container_title	Frontiers in Molecular Biosciences
authorswithroles_txt_mv	Lisa Maria Metz @@aut@@ Tobias Feige @@aut@@ Larissa de Biasi @@aut@@ Agnes Ehrenberg @@aut@@ Joscha Mulorz @@aut@@ Laura Mara Toska @@aut@@ Friedrich Reusswig @@aut@@ Christine Quast @@aut@@ Norbert Gerdes @@aut@@ Malte Kelm @@aut@@ Hubert Schelzig @@aut@@ Margitta Elvers @@aut@@
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topic_title	QH301-705.5 Platelet pannexin-1 channels modulate neutrophil activation and migration but not the progression of abdominal aortic aneurysm platelets pannexin-1 abdominal aortic aneurysm inflammation ECM remodeling selectin
topic	misc QH301-705.5 misc platelets misc pannexin-1 misc abdominal aortic aneurysm misc inflammation misc ECM remodeling misc selectin misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc platelets misc pannexin-1 misc abdominal aortic aneurysm misc inflammation misc ECM remodeling misc selectin misc Biology (General)
topic_browse	misc QH301-705.5 misc platelets misc pannexin-1 misc abdominal aortic aneurysm misc inflammation misc ECM remodeling misc selectin misc Biology (General)
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title	Platelet pannexin-1 channels modulate neutrophil activation and migration but not the progression of abdominal aortic aneurysm
ctrlnum	(DE-627)DOAJ08826985X (DE-599)DOAJ715ce80f15904c5caf775f925230aee4
title_full	Platelet pannexin-1 channels modulate neutrophil activation and migration but not the progression of abdominal aortic aneurysm
author_sort	Lisa Maria Metz
journal	Frontiers in Molecular Biosciences
journalStr	Frontiers in Molecular Biosciences
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author_browse	Lisa Maria Metz Tobias Feige Larissa de Biasi Agnes Ehrenberg Joscha Mulorz Laura Mara Toska Friedrich Reusswig Christine Quast Norbert Gerdes Malte Kelm Hubert Schelzig Margitta Elvers
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author-letter	Lisa Maria Metz
doi_str_mv	10.3389/fmolb.2023.1111108
author2-role	verfasserin
title_sort	platelet pannexin-1 channels modulate neutrophil activation and migration but not the progression of abdominal aortic aneurysm
callnumber	QH301-705.5
title_auth	Platelet pannexin-1 channels modulate neutrophil activation and migration but not the progression of abdominal aortic aneurysm
abstract	Abdominal aortic aneurysm (AAA) is a common disease and highly lethal if untreated. The progressive dilatation of the abdominal aorta is accompanied by degradation and remodeling of the vessel wall due to chronic inflammation. Pannexins represent anion-selective channels and play a crucial role in non-vesicular ATP release to amplify paracrine signaling in cells. Thus, pannexins are involved in many (patho-) physiological processes. Recently, Panx1 channels were identified to be significantly involved in abdominal aortic aneurysm formation through endothelial derived Panx1 regulated inflammation and aortic remodeling. In platelets, Panx1 becomes activated following activation of glycoprotein (GP) VI. Since platelets play a role in cardiovascular diseases including abdominal aortic aneurysm, we analyzed the contribution of platelet Panx1 in the progression of abdominal aortic aneurysm. We detected enhanced Panx1 plasma levels in abdominal aortic aneurysm patients. In experimental abdominal aortic aneurysm using the pancreatic porcine elastase (PPE) mouse model, a major contribution of platelet Panx1 channels in platelet activation, pro-coagulant activity of platelets and platelet-mediated inflammation has been detected. In detail, platelets are important for the migration of neutrophils into the aortic wall induced by direct cell interaction and by activation of endothelial cells. Decreased platelet activation and inflammation did not affect ECM remodeling or wall thickness in platelet-specific Panx1 knock-out mice following PPE surgery. Thus, aortic diameter expansion at different time points after elastase infusion of the aortic wall was unaltered in platelet-specific Panx1 deficient mice suggesting that the modulation of inflammation alone does not affect abdominal aortic aneurysm formation and progression. In conclusion, our data strongly supports the role of platelets in inflammatory responses in abdominal aortic aneurysm via Panx1 channels and adds important knowledge about the significance of platelets in abdominal aortic aneurysm pathology important for the establishment of an anti-platelet therapy for abdominal aortic aneurysm patients.
abstractGer	Abdominal aortic aneurysm (AAA) is a common disease and highly lethal if untreated. The progressive dilatation of the abdominal aorta is accompanied by degradation and remodeling of the vessel wall due to chronic inflammation. Pannexins represent anion-selective channels and play a crucial role in non-vesicular ATP release to amplify paracrine signaling in cells. Thus, pannexins are involved in many (patho-) physiological processes. Recently, Panx1 channels were identified to be significantly involved in abdominal aortic aneurysm formation through endothelial derived Panx1 regulated inflammation and aortic remodeling. In platelets, Panx1 becomes activated following activation of glycoprotein (GP) VI. Since platelets play a role in cardiovascular diseases including abdominal aortic aneurysm, we analyzed the contribution of platelet Panx1 in the progression of abdominal aortic aneurysm. We detected enhanced Panx1 plasma levels in abdominal aortic aneurysm patients. In experimental abdominal aortic aneurysm using the pancreatic porcine elastase (PPE) mouse model, a major contribution of platelet Panx1 channels in platelet activation, pro-coagulant activity of platelets and platelet-mediated inflammation has been detected. In detail, platelets are important for the migration of neutrophils into the aortic wall induced by direct cell interaction and by activation of endothelial cells. Decreased platelet activation and inflammation did not affect ECM remodeling or wall thickness in platelet-specific Panx1 knock-out mice following PPE surgery. Thus, aortic diameter expansion at different time points after elastase infusion of the aortic wall was unaltered in platelet-specific Panx1 deficient mice suggesting that the modulation of inflammation alone does not affect abdominal aortic aneurysm formation and progression. In conclusion, our data strongly supports the role of platelets in inflammatory responses in abdominal aortic aneurysm via Panx1 channels and adds important knowledge about the significance of platelets in abdominal aortic aneurysm pathology important for the establishment of an anti-platelet therapy for abdominal aortic aneurysm patients.
abstract_unstemmed	Abdominal aortic aneurysm (AAA) is a common disease and highly lethal if untreated. The progressive dilatation of the abdominal aorta is accompanied by degradation and remodeling of the vessel wall due to chronic inflammation. Pannexins represent anion-selective channels and play a crucial role in non-vesicular ATP release to amplify paracrine signaling in cells. Thus, pannexins are involved in many (patho-) physiological processes. Recently, Panx1 channels were identified to be significantly involved in abdominal aortic aneurysm formation through endothelial derived Panx1 regulated inflammation and aortic remodeling. In platelets, Panx1 becomes activated following activation of glycoprotein (GP) VI. Since platelets play a role in cardiovascular diseases including abdominal aortic aneurysm, we analyzed the contribution of platelet Panx1 in the progression of abdominal aortic aneurysm. We detected enhanced Panx1 plasma levels in abdominal aortic aneurysm patients. In experimental abdominal aortic aneurysm using the pancreatic porcine elastase (PPE) mouse model, a major contribution of platelet Panx1 channels in platelet activation, pro-coagulant activity of platelets and platelet-mediated inflammation has been detected. In detail, platelets are important for the migration of neutrophils into the aortic wall induced by direct cell interaction and by activation of endothelial cells. Decreased platelet activation and inflammation did not affect ECM remodeling or wall thickness in platelet-specific Panx1 knock-out mice following PPE surgery. Thus, aortic diameter expansion at different time points after elastase infusion of the aortic wall was unaltered in platelet-specific Panx1 deficient mice suggesting that the modulation of inflammation alone does not affect abdominal aortic aneurysm formation and progression. In conclusion, our data strongly supports the role of platelets in inflammatory responses in abdominal aortic aneurysm via Panx1 channels and adds important knowledge about the significance of platelets in abdominal aortic aneurysm pathology important for the establishment of an anti-platelet therapy for abdominal aortic aneurysm patients.
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title_short	Platelet pannexin-1 channels modulate neutrophil activation and migration but not the progression of abdominal aortic aneurysm
url	https://doi.org/10.3389/fmolb.2023.1111108 https://doaj.org/article/715ce80f15904c5caf775f925230aee4 https://www.frontiersin.org/articles/10.3389/fmolb.2023.1111108/full https://doaj.org/toc/2296-889X
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author2	Tobias Feige Larissa de Biasi Agnes Ehrenberg Joscha Mulorz Laura Mara Toska Friedrich Reusswig Christine Quast Norbert Gerdes Malte Kelm Hubert Schelzig Margitta Elvers
author2Str	Tobias Feige Larissa de Biasi Agnes Ehrenberg Joscha Mulorz Laura Mara Toska Friedrich Reusswig Christine Quast Norbert Gerdes Malte Kelm Hubert Schelzig Margitta Elvers
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up_date	2024-07-03T16:46:22.632Z
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